管必冲凝胶膏配方的优化及其促进大鼠骨折模型血管生成的作用

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2025-10-01 DOI:10.1016/j.jtcme.2024.08.009

Yu-Ying Deng , Bing-Hao Shao , Yi-Bo Wang, Jing Wang, Ze-Feng Zhang, Xue-Qing Xie, Xue Wei, Xing Chen, Chun-Mei Wang

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引用次数: 0

摘要

背景与目的经皮给药在中药中已有几千年的历史，值得进一步发展。本研究的目的是研究管必冲凝胶石膏（TGP）的制备方法，并评价其促进骨折愈合的活性。实验方法：以大鼠皮肤为屏障，以接受液对人脐静脉内皮细胞（HUVECs）增殖促进率为评价指标，采用Franz扩散细胞法研究石膏的体外通透性。采用改良的Einhorn方法建立大鼠胫骨骨折模型。通过x线摄影监测骨愈合过程。采用Safranin O Fast Green染色和CD31免疫组化检测大鼠骨发育和血管生成情况。ELISA法测定血清骨代谢物及VEGF水平。结果与结论氮酮治疗TGP的最佳剂量为2%。这种膏药的透皮渗透方程与里格-佩帕斯方程一致。制备的石膏至少稳定3个月。x线图像显示，TGP在各时期均以剂量相关的形式促进骨折愈合。血清BALP、OC、CTX-I和VEGF水平也表明治疗后骨折愈合较好。TGP在第7、14、21天显著刺激骨折部位血管生成和骨生长，并在第3天显示出促进骨生长的作用。这些数据证明制备的管必冲凝胶膏剂可以通过促进血管生成来促进骨折愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

$Optimization of the formulation of Tubiechong gel plaster and its effect on fracture healing by promoting angiogenesis in a rat model$

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Optimization of the formulation of Tubiechong gel plaster and its effect on fracture healing by promoting angiogenesis in a rat model

Background and aim

Transdermal drug delivery has been used in traditional Chinese medicine for thousands of years and is worth further developing. The purpose of this study was to investigate the preparation method of Tubiechong gel plaster (TGP) and evaluate its activity to promote fracture healing.

Experimental procedure

Using rat skin as a barrier, and the human umbilical vein endothelial cells (HUVECs) proliferation promotion rate of the receiving solution as the evaluation index, the in vitro permeability of the plaster was studied by Franz diffusion cell method. A modified Einhorn method was used to model rat tibia fractures. The bone healing process was monitored by radiography. Bone development and angiogenesis were assessed by Safranin O Fast Green staining and CD31 immunohistochemistry, respectively. Serum bone metabolites and VEGF levels were determined by ELISA method.

Results and conclusion

The optimal dose of azone for TGP is 2 %. The transdermal penetration equation for this plaster is consistent with the Ritger-Peppas equation. The prepared plaster was stable for at least 3 months. X-ray images showed that the fracture healing was promoted by TGP in a dose-related form at all periods. Serum BALP, OC, CTX-I, and VEGF levels also indicated better fracture healing after treatment. TGP significantly stimulated angiogenesis and bone growth at the fracture site on days 7,14 and 21, and the effect of promoting bone growth was shown on the third day. These data prove that the prepared Tubiechong gel plasters can promote fracture healing by promoting angiogenesis.

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.