Journal of Traditional and Complementary Medicine最新文献

{"title":"A comprehensive review on ethnomedicinal uses, phytochemistry, toxicology, and pharmacological activities of Dittrichia viscosa (L.) Greuter","authors":"Rania Jerada ,&nbsp;Abdeljalil Er-Rakibi ,&nbsp;Abha Cherkani Hassani ,&nbsp;Hanane Benzeid ,&nbsp;Abdelmoula El Ouardi ,&nbsp;Hicham Harhar ,&nbsp;Bey Hing Goh ,&nbsp;Yoon-Yen Yow ,&nbsp;Hooi-Leng Ser ,&nbsp;Abdelhakim Bouyahya ,&nbsp;Brahim Mojemmi ,&nbsp;Anass Doukkali","doi":"10.1016/j.jtcme.2024.03.012","DOIUrl":"10.1016/j.jtcme.2024.03.012","url":null,"abstract":"<div><p><em>Dittrichia viscosa</em> is a perennial herb that has been used for generations in traditional medicine to address a variety of diseases, including diabetes, hypertension, cancer, microbial disorders, inflammatory conditions, and wound healing. The objective of this review is to provide an overview of existing knowledge on <em>D. viscosa</em> with regards to its botanical description, ethnomedicinal uses, and pharmacological properties. Databases such as Scopus, Wiley-Online, PubMed, Springer, Google Scholar, and ScienceDirect were used to select relevant articles based on their title and abstract.</p><p>The reviewed studies found a strong correlation between <em>D. viscosa</em>'s traditional uses and its observed biological effects. Pharmacological research has shown that the essential oils and extracts from <em>D. viscosa</em> possess a variety of biological activities, such as anti-inflammatory, anticancer, antibacterial, antifungal, analgesic, and antioxidant properties. The chemical compounds found in <em>D. viscosa</em> include sesquiterpenes, monoterpenes, flavonoids, and phenolic acids; some of these compounds, such as tometosin and inuviscolide, have been isolated and displayed promising cytotoxic and anti-inflammatory activity.</p><p>The present review suggests that the pharmacological properties of <em>D. viscosa</em> align well with its ethnomedicinal uses. These findings support the traditional use of <em>D. viscosa</em> in treating various illnesses. Additionally, toxicological examinations of <em>D. viscosa</em> extracts and essential oil have demonstrated the plant's safety, which supports the need for comprehensive pharmacological studies, <em>in vivo</em> studies, and clinical trials to evaluate the best doses for optimal medicinal effects. This work underscores the medicinal value of <em>D. viscosa</em> and its potential in developing new pharmacological agents to address major health challenges like antibiotic resistance and cancers.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 355-380"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000324/pdfft?md5=7ba9811db09e71ac401faaac781fbcf1&pid=1-s2.0-S2225411024000324-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140272571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute toxicity profiling of medicinal herb Ardisia elliptica leaf extract by conventional evaluations and proton nuclear magnetic resonance (NMR) metabolomics","authors":"Pei Lou Wong ,&nbsp;Nur Khaleeda Zulaikha Zolkeflee ,&nbsp;Nurul Shazini Ramli ,&nbsp;Chin Ping Tan ,&nbsp;Azrina Azlan ,&nbsp;Faridah Abas","doi":"10.1016/j.jtcme.2024.02.001","DOIUrl":"10.1016/j.jtcme.2024.02.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>Interest in the safety of herbal medicine is growing rapidly regarding knowledge and challenges in natural products. Hence, this study aimed to reveal the toxicological profile of <em>Ardisia elliptica</em>, a traditional medicinal plant used in the treatment of various illnesses.</p></div><div><h3>Experimental procedure</h3><p>Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract, using a combination of conventional investigations and ¹H-NMR-based metabolomics approaches.</p></div><div><h3>Results</h3><p>Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. ¹H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, ¹H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.</p></div><div><h3>Conclusion</h3><p>This study suggested that 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 456-466"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000117/pdfft?md5=0cd1d2568e7879636a4f67485bf5bedf&pid=1-s2.0-S2225411024000117-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140463678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of stimulating single acupoint and combination acupoints on diabetic gastroparesis: A randomised controlled trial study","authors":"Renming Liu ,&nbsp;Min He ,&nbsp;Xuewei Zhao ,&nbsp;Mengmeng Sun ,&nbsp;Jiazhen Cao ,&nbsp;Xinyue Wang ,&nbsp;Xingbang Wang ,&nbsp;Shuming Zhao ,&nbsp;Fuchun Wang ,&nbsp;Tie Li","doi":"10.1016/j.jtcme.2024.01.008","DOIUrl":"10.1016/j.jtcme.2024.01.008","url":null,"abstract":"<div><h3>Background and aim</h3><p>The most effective among the acupoints remains to be determined for treating diabetic gastroparesis (DGP). This study aimed to compare single and combination acupoints for their effectiveness in DGP.</p></div><div><h3>Experimental procedure</h3><p>A prospective, patient-assessor-blinded randomised controlled trial was designed to compare the efficacy of 8-week acupuncture at a single acupoint (Zhongwan, CV-12), combination acupoints (Zhongwan, CV-12 and Zusanli, ST-36), and a sham-acupoint, in 99 adults with DGP. The primary clinical outcome was measured using the Gastroparesis Cardinal Symptom Index (GCSI), while barium meal examination, fasting plasma glucose, the 2-h plasma glucose, short-form health survey (SF-36), and GCSI subscales were performed for evaluating secondary clinical outcomes. These results were analysed by two factorial analysis of variance (ANOVA) test, Chi-Square, Fisher Exact, Kruskal–Wallis tests and Tukey's Honest Significant Difference (HSD) test.</p></div><div><h3>Results</h3><p>After randomization, 97 patients completed the study. GCSI scores of all groups decreased during both post-treatment and the follow-up period, they were statistically significant compared to the baseline period (<em>p</em> &lt; 0.01), but there was no significant difference among the groups (<em>p</em> &gt; 0.05) during the post-treatment period. GCSI scores improved more in the combination acupoints group than in the single acupoint group which was better than the sham group after treatment. During the follow-up period, the same trend was observed.</p></div><div><h3>Conclusions</h3><p>Among patients with DGP, the combination acupoints were more beneficial compared with single and sham acupoints.</p></div><div><h3>Trial registration number</h3><p>NCT02452489.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 446-455"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000087/pdfft?md5=2dd43bff8074cfa32227d692779c7b07&pid=1-s2.0-S2225411024000087-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139636381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red rice bran aqueous extract ameliorate diabetic status by inhibiting intestinal glucose transport in high fat diet/STZ-induced diabetic rats","authors":"Atcharaporn Ontawong ,&nbsp;Sirinat Pengnet ,&nbsp;Arthid Thim-Uam ,&nbsp;Chutima S. Vaddhanaphuti ,&nbsp;Narongsuk Munkong ,&nbsp;Manussaborn Phatsara ,&nbsp;Kullanat Kuntakhut ,&nbsp;Jakkapong Inchai ,&nbsp;Doungporn Amornlerdpison ,&nbsp;Teerawat Rattanaphot","doi":"10.1016/j.jtcme.2023.12.003","DOIUrl":"10.1016/j.jtcme.2023.12.003","url":null,"abstract":"<div><p>Red rice (<em>Oryza sativa</em> L.) consumption has grown recently, partly due to its potential health benefits in several disease prevention. The impact of red rice bran aqueous extract (RRBE) on intestinal glucose uptake and diabetes mellitus (DM) progression has not been thoroughly investigated. This study aimed to evaluate the effect of RRBE on <em>ex vivo</em> intestinal glucose absorption and its potential as an antihyperglycemic compound using a high-fat diet and streptozotocin (STZ)-induced diabetic rats. High-fat diet/STZ-induced diabetic rats were supplemented with either 1000 mg/kg body weight (BW) of RRBE, 70 mg/kg BW of metformin (Met), or a combination of RRBE and Met for 3 months. Plasma parameters, intestinal glucose transport, morphology, liver and soleus muscle glycogen accumulation were assessed. Treatment with RRBE, metformin, or combination markedly reversed hyperglycemia, hypertriglyceridemia, insulin resistance, and pancreatic morphology changes associated with T2DM. Correspondingly, all supplements effectively downregulated glucose transporters, resulting in a reduction of intestinal glucose transport—additionally, liver and soleus muscle glycogen accumulation was reduced in RRBE + Met treated group. Taken together, RRBE potentially suppressed intestinal glucose transporters' function and expression, reducing diabetic status.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 391-402"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001372/pdfft?md5=663a0e0f9b7bc8979fe2cc809ab8ed5e&pid=1-s2.0-S2225411023001372-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139188804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The therapeutic effect and metabolic mechanism analysis of Guilingji on idiopathic oligo-asthenoteratozoospermia","authors":"Yuming Feng ,&nbsp;Shanmeizi Zhao ,&nbsp;Rong Ju ,&nbsp;Jianbo Li ,&nbsp;Chengshuang Pan ,&nbsp;Qinglai Tang ,&nbsp;Zhichuan Zou ,&nbsp;Jun Jing ,&nbsp;Yao Xu ,&nbsp;Hualong Ding ,&nbsp;Jinzhao Ma ,&nbsp;Zhou Li ,&nbsp;Ting Tang ,&nbsp;Yong Shao ,&nbsp;Li Chen ,&nbsp;Xuefeng Huang ,&nbsp;Xiaohong Wang ,&nbsp;Bing Yao","doi":"10.1016/j.jtcme.2024.01.001","DOIUrl":"10.1016/j.jtcme.2024.01.001","url":null,"abstract":"<div><h3>Introduction</h3><p>Guilingji, a famous traditional Chinese medicine (TCM) formula, has been used to combat aging and male sexual dysfunction in China for centuries. To date, there has been little evidence-based clinical research on the use of Guilingji to treat idiopathic oligo-asthenoteratozoospermia (OAT), and the therapeutic mechanism from a metabolic perspective needs to be investigated further.</p></div><div><h3>Methods</h3><p>This was a multicenter, double-blind, randomized controlled clinical study of 240 patients with idiopathic OAT recruited from four hospitals between January 2020 and January 2022. Patients were randomly assigned in a 1꞉1 ratio to receive oral Guilingji capsules or placebo for 12 weeks. The total progressive motile sperm count (TPMSC) was considered the primary outcome, and the other sperm parameters, seminal plasma parameters and serum hormones were considered the secondary outcome. A nontargeted metabolomics analysis of serum from OAT patients before and after Guilingji administration was performed by HPLC–MS to identify key metabolites. Furthermore, we used a rat model to show spermatogenesis phenotypes to validate the effect of the key metabolites screened from the patients.</p></div><div><h3>Results</h3><p>At weeks 4, 8 and 12, TPMSC and other sperm parameters were significantly improved in the Guilingji group compared with the placebo group (<em>P</em> &lt; 0.05 for all comparisons). At week 4, superoxide dismutase (SOD) and acrosomal enzyme activity of seminal plasma were significantly elevated in the Guilingji group compared with the placebo group, while reactive oxygen species (ROS) levels were significantly reduced (<em>P</em> &lt; 0.05). Lactate dehydrogenase-X (LDHX) levels appeared to be significantly increased after 12 weeks continuous medication compared with Placebo group (<em>P</em> = 0.032). The metabolomics analysis of serum from OAT patients before and after Guilingji administration showed that the glucose-6-phosphate (G6P) concentration in patients' serum was significantly elevated after Guilingji treatment. Compared to the control, when Kidney-Yang deficiency model rats were treated with Guilingji or its key intermediate metabolite G6P, their sperm concentration and spermatozoic activity were improved similarly, and their structural damage of rat's testicular and epididymal tissues were recovered.</p></div><div><h3>Conclusion</h3><p>This study provided valuable clinical evidence for the utility of Guilingji as a treatment for OAT. These findings thus demonstrate that G6P is involved in the therapeutic mechanism of Guilingji in OAT treatment based on clinical and rat intervention studies.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 403-413"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000014/pdfft?md5=1beea949ce36b7b4c117aec01a97eabc&pid=1-s2.0-S2225411024000014-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139391853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crocin elicits potent anti-inflammatory and fibrinolytic properties post tendon injury, A new molecule for adhesion therapy","authors":"","doi":"10.1016/j.jtcme.2024.06.001","DOIUrl":"10.1016/j.jtcme.2024.06.001","url":null,"abstract":"<div><h3>Background</h3><div>Post-surgical tendon adhesion formation is a frequent clinical complication with limited treatment options. The aim of this study is to investigate safety and efficacy of orally administration of crocin in attenuating post-operative tendon-sheath adhesion bands in an Achilles tendon rat model.</div></div><div><h3>Methods</h3><div>Structural, mechanical, histological, and biochemical properties of Achilles tendons were analyzed in the presence and absence of crocin. Inflammation and total fibrosis of tendon tissues were graded between groups using macroscopic and histological scoring methods.</div></div><div><h3>Results</h3><div>Crocin significantly alleviated the severity, length, and density of Achilles tendon adhesions. Moreover, the recruitment of inflammatory cells and inflammation were significantly decreased in post-operative tissue samples of the crocin-treated group, as quantified with Moran scoring system. Histological results showed that crocin elicited a potent anti-fibrotic effect on tendon tissue samples as visualized by decreasing quantity, quality, grading of fibers, and collagen deposition at the site of surgery when scored either by Tang or Ishiyama grading systems. The H&amp;E staining showed no histo-pathological changes or damage to heart, kidney, and liver tissues of treated rats.</div></div><div><h3>Conclusion</h3><div>Our results showed that crocin is a safe effective therapeutic candidate with potent anti-inflammatory and anti-fibrotic properties for adhesion band therapy post tendon surgery.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 687-696"},"PeriodicalIF":3.3,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141407677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo evaluation of Andrographis paniculata and Boesenbergia rotunda extract activity against SARS-CoV-2 Delta variant in Golden Syrian hamsters: Potential herbal alternative for COVID-19 treatment","authors":"","doi":"10.1016/j.jtcme.2024.05.004","DOIUrl":"10.1016/j.jtcme.2024.05.004","url":null,"abstract":"<div><div>The ongoing COVID-19 pandemic has triggered extensive research, mainly focused on identifying effective therapeutic agents, specifically those targeting highly pathogenic SARS-CoV-2 variants. This study aimed to investigate the <em>in vivo</em> antiviral efficacy and anti-inflammatory activity of herbal extracts derived from <em>Andrographis paniculata</em> and <em>Boesenbergia rotunda</em>, using a Golden Syrian hamster model infected with Delta, a representative variant associated with severe COVID-19. Hamsters were intranasally inoculated with the SARS-CoV-2 Delta variant and orally administered either vehicle control, <em>B. rotunda</em>, or <em>A.</em> <em>paniculata</em> extract at a dosage of 1000 mg/kg/day. Euthanasia was conducted on days 1, 3, and 7 post-inoculation, with 4 animals per group. The results demonstrated that oral administration of <em>A. paniculata</em> extract significantly alleviated both lethality and infection severity compared with the vehicle control and <em>B. rotunda</em> extract. However, neither extract exhibited direct antiviral activity in terms of reducing viral load in the lungs. Nonetheless, <em>A. paniculata</em> extract treatment significantly reduced IL-6 protein levels in the lung tissue (7278 ± 868.4 pg/g tissue) compared to the control (12,495 ± 1118 pg/g tissue), indicating there was a decrease in local inflammation. This finding is evidenced by the ability of <em>A. paniculata</em> extract to reduce histological lesions in the lungs of infected hamsters. Furthermore, both extracts significantly decreased IL-6 and IP-10 mRNA expression in peripheral blood mononuclear cells of infected hamsters compared to the control group, suggesting systemic anti-inflammatory effects occurred. In conclusion, <em>A. paniculata</em> extract's potential therapeutic application for SARS-CoV-2 arises from its observed capacity to lessen inflammatory cytokine concentrations and mitigate lung pathology.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 598-610"},"PeriodicalIF":3.3,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141043060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of luteolin: A natural migraine management approach through network pharmacology","authors":"","doi":"10.1016/j.jtcme.2024.04.011","DOIUrl":"10.1016/j.jtcme.2024.04.011","url":null,"abstract":"<div><h3>Background</h3><div>Luteolin, a natural flavonoid, exhibits antioxidant and anti-inflammatory properties and has been investigated for potential health benefits. Its focus on migraine management arises from its ability to mitigate neuroinflammation, a key factor in migraine attacks.</div></div><div><h3>Methods</h3><div>pkCSM and Swiss ADME were employed to assess luteolin's pharmacokinetic properties, revealing challenges such as low water solubility and limited skin permeability. OSIRIS Property Explorer is used to check the toxicity. Ligand binding simulations indicated luteolin's potential to interact with calcitonin gene related peptide proteins, crucial in migraine pathophysiology. DisGeNet identified common targets related to migraine, with subsequent network analysis emphasizing promising targets.</div></div><div><h3>Results and Discussion</h3><div>Luteolin demonstrated good intestinal absorption but faced BBB limitations, suggesting a potential for oral administration but questioning direct brain impact. Nanoformulation was proposed to address solubility challenges, emphasizing the need for in vivo validation. The highest binding affinity with CGRP proteins PDBID: 6PFO (−7.63 kcal/mol) suggested a potential for migraine treatment, requiring empirical confirmation. Enrichment network analysis illustrated luteolin's potential in migraine treatment, emphasizing key targets such as PTGS2, AKT1, ESR1, MMP2, and MMP9. Luteolin shows promise for migraine management, evident in its pharmacokinetic, toxicological profiles, and interactions with CGRP proteins. Challenges like low solubility suggest the need for nanoformulations and empirical validation. Target identification and network analysis offer insights, highlighting potential therapeutic avenues in migraine treatment.</div></div><div><h3>Conclusion</h3><div>Luteolin holds promise in migraine management, necessitating further research for translation into effective interventions, considering its neuroprotective potential in broader neurological conditions.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 611-621"},"PeriodicalIF":3.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141030917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the mechanistic potential of Thuja occidentalis for managing diabetic neuropathy and nephropathy","authors":"","doi":"10.1016/j.jtcme.2024.04.009","DOIUrl":"10.1016/j.jtcme.2024.04.009","url":null,"abstract":"<div><div>Diabetes mellitus and its debilitating microvascular complications, including diabetic neuropathy and nephropathy, represent a growing global health burden. Despite advances in conventional therapies, their suboptimal efficacy and adverse effects necessitate exploring complementary and alternative medicine approaches. <em>Thuja occidentalis</em>, a coniferous tree species native to eastern North America, has gained significant attention for its potential therapeutic applications in various disorders, attributed to its rich phytochemical composition. The present comprehensive review evaluates the therapeutic potential of <em>Thuja occidentalis</em> in managing diabetic neuropathy and nephropathy, with a particular emphasis on elucidating the underlying cellular and molecular mechanisms. The review delves into the active constituents of <em>Thuja occidentalis</em>, such as essential oils, flavonoids, tannins, and proanthocyanidin compounds, which have demonstrated antioxidant, anti-inflammatory, and other beneficial properties in preclinical studies. Importantly, the review provides an in-depth analysis of the intricate signaling pathways modulated by <em>Thuja occidentalis,</em> including NF-κB, PI3K-Akt, JAK-STAT, JNK, MAPK/ERK, and Nrf2 cascades. These pathways are intricately linked to oxidative stress, inflammation, and apoptosis processes, which play pivotal roles in the pathogenesis of diabetic neuropathy and nephropathy. Furthermore, the review critically evaluates the evidence-based toxicological data of <em>Thuja occidentalis</em> as a more effective and comprehensive therapeutic strategy in diabetes complications. Therefore, the current review aims to provide a comprehensive understanding of the therapeutic potential of <em>Thuja occidentalis</em> as an adjunctive treatment strategy for diabetic neuropathy and nephropathy while highlighting the need for further research to optimize its clinical translation.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 581-597"},"PeriodicalIF":3.3,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cannabidiol suppresses proliferation and induces cell death, autophagy and senescence in human cholangiocarcinoma cells via the PI3K/AKT/mTOR pathway","authors":"","doi":"10.1016/j.jtcme.2024.04.007","DOIUrl":"10.1016/j.jtcme.2024.04.007","url":null,"abstract":"<div><h3>Background and aim</h3><div>Cholangiocarcinoma (CCA) is usually diagnosed at a late stage, leading to treatment failure. Cannabidiol (CBD), exhibits diverse anti-cancer effects in various cancers, offering avenues for improving CCA treatment. This study investigated the effects of CBD on human CCA cells and the underlying mechanisms <em>in vitro</em> and <em>in vivo</em>.</div></div><div><h3>Experimental procedure</h3><div>The effects of CBD on three CCA cell lines (KKU-213B, KKU-100, KKU-055) were assessed using the SRB assay, clonogenic assay, cell cycle arrest, and 3D holotomography. Morphological changes were examined using transmission electron microscopy, while mitochondrial ROS levels and mitochondrial membrane potential were studied using MitoSOX, JC-1, and DCFH-DA. Cellular senescence induction was evaluated via SA-β-gal staining. Protein associatedwith autophagy and cellular senescence were analyzed using Western blot and/or immunofluorescent assays. A xenograft model demonstrated the anti-tumor activity of CBD and the induction of cellular senescence through immunohistochemistry targeting PCNA, β-gal, and p21.</div></div><div><h3>Results and conclusion</h3><div>CBD effectively inhibited CCA cell proliferation, suppressed colony formation and induced G0/G1 phase cell cycle arrest. Morphological examination revealed lipid droplets/vesicles in CCA cell lines. CBD induced autophagy by upregulating LC3BII, downregulating p62, and inhibiting the <em>p</em>-PI3K, <em>p</em>-AKT, and <em>p</em>-mTOR pathways. Additionally, CBD disrupted mitochondrial homeostasis by elevating ROS, reducing membrane potential, and induced cellular senescence by increasing the expression of p53 and p21. <em>In-vitro</em> results were confirmed by xenograft models. Overall, CBD suppresses proliferation and induces cell death, autophagy and senescence in CCA cells via the PI3K/AKT/mTOR pathway, which indicates a therapeutic option for CCA treatment.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 6","pages":"Pages 622-634"},"PeriodicalIF":3.3,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140762438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}