Achillea 提取物通过调节炎症细胞因子引发抗糖尿病神经病理性疼痛

IF 3 3区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Ola Kbaydet , Maha Abou-Ela , Karim Raafat
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引用次数: 0

摘要

背景和目的东地中海草药因其免疫调节潜力而被利用。水蛭属植物具有民族药理学意义。本研究旨在对东地中海地区的三种阿喀琉叶(Achillea kotschyi Boiss)进行深入的植物化学研究。(AK)、falcata L. (AF)和aleppica DC。(AAL)精油(EOs),并研究它们的抗糖尿病、抗神经性疼痛和炎症,并深入了解它们的作用机制。实验方法包括从三种被检测物种的三个不同样品中分离eo,优化和验证GC-MS方法,体内抗氧化技术,糖化血红蛋白糖尿病评估,胰岛素水平,热痛觉过敏和触觉异常性疼痛实验,抗炎和炎症标志物,TNF-α, IL-6和10水平监测。结果与结论建立了优化且有效的气相色谱-质谱定量方法。AK-EO中检测到的最高成分为图酮(35.84±0.01 %)、α-香檀烯(15.99±0.01 %)和辛美烯(12.31±0.01 %),AF中检测到的最高成分为辛美烯(65.11±0.01 %)、图美烯(14.68±0.01 %)和间苯二酚(5.00±0.01 %),AAL-EO中检测到的最高成分为间甲苯酰胺(49.35±0.01 %)、桉油醇(21.08±0.01 %)和辛美烯(13.48±0.01 %)。AAL-EO在使hba1c水平正常化方面具有优势。AAL-EO对热痛觉过敏和触觉异常性疼痛潜伏期的改善最大。胰岛素分泌潜能和抗氧化血清过氧化氢酶水平的提高可能是其抗伤机制。与AK和AF-EOs相比,AAL-EO最高剂量(300 mg/kg)在急性和慢性抗炎潜能方面均具有优势。炎症介质TNF-α和IL-6的降低和IL-10滴度的提高被证明是其抗炎机制。总之,AK, AF和AAL被证明含有丰富的精油成分,可以改善慢性疾病,如糖尿病,糖尿病神经病变疼痛和炎症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Achillea extracts elicit anti-diabetic neuropathic pain by modulating inflammatory cytokines

Achillea extracts elicit anti-diabetic neuropathic pain by modulating inflammatory cytokines

Background and aim

The Eastern-Mediterranean herbal-remedies are utilized for their immunomodulatory-potentials. Achillea species have ethno-pharmacological significance. The current-work aims to perform an in-depth phytochemical-investigation on three Eastern-Mediterranean Achillea species, mainly, Achillea kotschyi Boiss. (AK), Achillea falcata L. (AF), and Achillea aleppica DC. (AAL) essential oils (EOs), and to investigate their antidiabetic, and antineuropathic pain, and inflammation, and to have insights into their mechanisms of action.

Experimental-procedure

The methods include EO-isolation from three different samples of each of the three species examined, optimization, and validation of GC-MS method, in-vivo antioxidant-technique, HbA1c diabetes-assessment, insulin-level, thermal-hyperalgesia and tactile-allodynia experiments, anti-inflammatory, and inflammatory-markers, TNF-α, IL-6, and 10 level monitoring.

Results and conclusion

The optimized and validated GC-MS method was developed to quantify the major EO components. The highest components detected on AK-EO were thujone (35.84 ± 0.01 %), α-phellandrene (15.99 ± 0.01), and ocimene (12.31 ± 0.01 %), while those of AF were ocimene (65.11 ± 0.01 %), thujone (14.68 ± 0.01 %), and resorcinol (5.00 ± 0.01 %), and the AAL-EO were m-toluamide (49.35 ± 0.01 %), eucalyptol (21.08 ± 0.01 %), and ocimene (13.48 ± 0.01 %). AAL-EO showed superiority in normalizing HbA1c-levels. AAL-EO showed the highest improvement in both thermal-hyperalgesia and tactile-allodynia latencies. The insulin-secretagogue-potential and the improvement of the antioxidant serum-catalase levels might be their mechanism of antinociception. AAL-EO highest-dose (300 mg/kg)showed superiority in the anti-inflammatory-potentials both acutely and chronically, compared to AK and AF-EOs. The decrease in the inflammatory-mediators, TNF-α and IL-6, and the improvement of IL-10 titers proved to be their mechanism of anti-inflammation. In conclusion, AK, AF, and AAL proved to be rich in essential-oil components that can ameliorate chronic-conditions like diabetes, diabetic-neuropathy pain, and inflammation.
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来源期刊
Journal of Traditional and Complementary Medicine
Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine
CiteScore
9.30
自引率
6.70%
发文量
78
审稿时长
66 days
期刊介绍: eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.
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