{"title":"Achillea 提取物通过调节炎症细胞因子引发抗糖尿病神经病理性疼痛","authors":"Ola Kbaydet , Maha Abou-Ela , Karim Raafat","doi":"10.1016/j.jtcme.2024.04.012","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and aim</h3><div>The Eastern-Mediterranean herbal-remedies are utilized for their immunomodulatory-potentials. <em>Achillea</em> species have ethno-pharmacological significance. The current-work aims to perform an in-depth phytochemical-investigation on three Eastern-Mediterranean <em>Achillea species,</em> mainly, <em>Achillea kotschyi</em> Boiss. (AK)<em>, Achillea falcata</em> L. (AF)<em>,</em> and <em>Achillea aleppica</em> DC. (AAL) essential oils (EOs), and to investigate their antidiabetic, and antineuropathic pain, and inflammation, and to have insights into their mechanisms of action.</div></div><div><h3>Experimental-procedure</h3><div>The methods include EO-isolation from three different samples of each of the three species examined, optimization, and validation of GC-MS method, <em>in</em>-<em>vivo</em> antioxidant-technique, HbA1c diabetes-assessment, insulin-level, thermal-hyperalgesia and tactile-allodynia experiments, anti-inflammatory, and inflammatory-markers, TNF-α, IL-6, and 10 level monitoring.</div></div><div><h3>Results and conclusion</h3><div>The optimized and validated GC-MS method was developed to quantify the major EO components. The highest components detected on AK-EO were thujone (35.84 ± 0.01 %), <em>α</em>-phellandrene (15.99 ± 0.01), and ocimene (12.31 ± 0.01 %), while those of AF were ocimene (65.11 ± 0.01 %), thujone (14.68 ± 0.01 %), and resorcinol (5.00 ± 0.01 %), and the AAL-EO were <em>m</em>-toluamide (49.35 ± 0.01 %), eucalyptol (21.08 ± 0.01 %), and ocimene (13.48 ± 0.01 %). AAL-EO showed superiority in normalizing HbA1c-levels. AAL-EO showed the highest improvement in both thermal-hyperalgesia and tactile-allodynia latencies. The insulin-secretagogue-potential and the improvement of the antioxidant serum-catalase levels might be their mechanism of antinociception. AAL-EO highest-dose (300 mg/kg)showed superiority in the anti-inflammatory-potentials both acutely and chronically, compared to AK and AF-EOs. The decrease in the inflammatory-mediators, TNF-α and IL-6, and the improvement of IL-10 titers proved to be their mechanism of anti-inflammation. In conclusion, AK, AF, and AAL proved to be rich in essential-oil components that can ameliorate chronic-conditions like diabetes, diabetic-neuropathy pain, and inflammation.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 4","pages":"Pages 388-403"},"PeriodicalIF":3.0000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Achillea extracts elicit anti-diabetic neuropathic pain by modulating inflammatory cytokines\",\"authors\":\"Ola Kbaydet , Maha Abou-Ela , Karim Raafat\",\"doi\":\"10.1016/j.jtcme.2024.04.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background and aim</h3><div>The Eastern-Mediterranean herbal-remedies are utilized for their immunomodulatory-potentials. <em>Achillea</em> species have ethno-pharmacological significance. The current-work aims to perform an in-depth phytochemical-investigation on three Eastern-Mediterranean <em>Achillea species,</em> mainly, <em>Achillea kotschyi</em> Boiss. (AK)<em>, Achillea falcata</em> L. (AF)<em>,</em> and <em>Achillea aleppica</em> DC. (AAL) essential oils (EOs), and to investigate their antidiabetic, and antineuropathic pain, and inflammation, and to have insights into their mechanisms of action.</div></div><div><h3>Experimental-procedure</h3><div>The methods include EO-isolation from three different samples of each of the three species examined, optimization, and validation of GC-MS method, <em>in</em>-<em>vivo</em> antioxidant-technique, HbA1c diabetes-assessment, insulin-level, thermal-hyperalgesia and tactile-allodynia experiments, anti-inflammatory, and inflammatory-markers, TNF-α, IL-6, and 10 level monitoring.</div></div><div><h3>Results and conclusion</h3><div>The optimized and validated GC-MS method was developed to quantify the major EO components. The highest components detected on AK-EO were thujone (35.84 ± 0.01 %), <em>α</em>-phellandrene (15.99 ± 0.01), and ocimene (12.31 ± 0.01 %), while those of AF were ocimene (65.11 ± 0.01 %), thujone (14.68 ± 0.01 %), and resorcinol (5.00 ± 0.01 %), and the AAL-EO were <em>m</em>-toluamide (49.35 ± 0.01 %), eucalyptol (21.08 ± 0.01 %), and ocimene (13.48 ± 0.01 %). AAL-EO showed superiority in normalizing HbA1c-levels. AAL-EO showed the highest improvement in both thermal-hyperalgesia and tactile-allodynia latencies. The insulin-secretagogue-potential and the improvement of the antioxidant serum-catalase levels might be their mechanism of antinociception. AAL-EO highest-dose (300 mg/kg)showed superiority in the anti-inflammatory-potentials both acutely and chronically, compared to AK and AF-EOs. The decrease in the inflammatory-mediators, TNF-α and IL-6, and the improvement of IL-10 titers proved to be their mechanism of anti-inflammation. In conclusion, AK, AF, and AAL proved to be rich in essential-oil components that can ameliorate chronic-conditions like diabetes, diabetic-neuropathy pain, and inflammation.</div></div>\",\"PeriodicalId\":17449,\"journal\":{\"name\":\"Journal of Traditional and Complementary Medicine\",\"volume\":\"15 4\",\"pages\":\"Pages 388-403\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional and Complementary Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2225411024000567\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional and Complementary Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2225411024000567","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Achillea extracts elicit anti-diabetic neuropathic pain by modulating inflammatory cytokines
Background and aim
The Eastern-Mediterranean herbal-remedies are utilized for their immunomodulatory-potentials. Achillea species have ethno-pharmacological significance. The current-work aims to perform an in-depth phytochemical-investigation on three Eastern-Mediterranean Achillea species, mainly, Achillea kotschyi Boiss. (AK), Achillea falcata L. (AF), and Achillea aleppica DC. (AAL) essential oils (EOs), and to investigate their antidiabetic, and antineuropathic pain, and inflammation, and to have insights into their mechanisms of action.
Experimental-procedure
The methods include EO-isolation from three different samples of each of the three species examined, optimization, and validation of GC-MS method, in-vivo antioxidant-technique, HbA1c diabetes-assessment, insulin-level, thermal-hyperalgesia and tactile-allodynia experiments, anti-inflammatory, and inflammatory-markers, TNF-α, IL-6, and 10 level monitoring.
Results and conclusion
The optimized and validated GC-MS method was developed to quantify the major EO components. The highest components detected on AK-EO were thujone (35.84 ± 0.01 %), α-phellandrene (15.99 ± 0.01), and ocimene (12.31 ± 0.01 %), while those of AF were ocimene (65.11 ± 0.01 %), thujone (14.68 ± 0.01 %), and resorcinol (5.00 ± 0.01 %), and the AAL-EO were m-toluamide (49.35 ± 0.01 %), eucalyptol (21.08 ± 0.01 %), and ocimene (13.48 ± 0.01 %). AAL-EO showed superiority in normalizing HbA1c-levels. AAL-EO showed the highest improvement in both thermal-hyperalgesia and tactile-allodynia latencies. The insulin-secretagogue-potential and the improvement of the antioxidant serum-catalase levels might be their mechanism of antinociception. AAL-EO highest-dose (300 mg/kg)showed superiority in the anti-inflammatory-potentials both acutely and chronically, compared to AK and AF-EOs. The decrease in the inflammatory-mediators, TNF-α and IL-6, and the improvement of IL-10 titers proved to be their mechanism of anti-inflammation. In conclusion, AK, AF, and AAL proved to be rich in essential-oil components that can ameliorate chronic-conditions like diabetes, diabetic-neuropathy pain, and inflammation.
期刊介绍:
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