Auricular acupuncture plays a neuroprotective role in 6-hydroxydopamine-induced Parkinson's disease in rats

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2024-05-28 DOI:10.1016/j.jtcme.2024.05.008

Huong Thi-Mai Nguyen , Der-Yen Lee , Ching-Liang Hsieh

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引用次数: 0

Abstract

Background

Parkinson's disease (PD) is the second-most common neurodegenerative disease. Currently, PD treatment is symptomatic and involves the use of dopamine-based therapies. This study investigated auricular acupuncture on motor and cognitive abilities in rats with 6-OHDA-induced PD.

Methods

A PD rat model was established by bilaterally injecting 6-OHDA into the lateral dorsal striatum. Then, 2- or 15-Hz auricular electroacupuncture (EA) was applied to the auricular CO15 and CO12 points bilaterally for 20 min three times a week for four consecutive weeks.

Results

Both the latency to fall and rest time of the open field test in the EA15 group were greater than in the 6-OHDA group (p < 0.001 and p < 0.05). The time spent on the two-object recognition task was greater in the EA15 group and EA2 group than in the 6-OHDA group (p < 0.01 and p < 0.05). More tyrosine hydroxylase (TH)-positive neurons and fibers were noted in the dorsolateral striatum and substantia nigra (SN) (all p < 0.05). TH expression in the SN was greater in the EA15 group than that in the 6-OHDA group (p < 0.05), while α-synuclein expression in the SN was stronger in the 6-OHDA group than in the EA15 group (p < 0.05). The l-DOPA level in the striatum was higher in the EA15 group than in the 6-OHDA group (p < 0.05).

Conclusion

According to the results, rats with 6-OHDA-induced PD may benefit from auricular EA in terms of motor and cognitive behavior as well as neuroprotection.

Abstract Image

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耳穴针刺对6-羟多巴胺诱导的帕金森病大鼠具有神经保护作用

帕金森病（PD）是第二常见的神经退行性疾病。目前，帕金森病的治疗是对症的，涉及到以多巴胺为基础的治疗。本研究探讨耳针对6-羟多巴胺诱导的帕金森病大鼠运动和认知能力的影响。方法采用双侧侧背纹状体注射6-羟色胺建立PD大鼠模型。然后，每周3次或15 hz耳穴电针（EA）在耳穴CO15和CO12穴双侧穿刺20 min，连续4周。结果EA15组裸地试验跌倒潜伏期和休息时间均大于6-OHDA组(p <；0.001和p <；0.05)。EA15组和EA2组在双目标识别任务上花费的时间大于6-OHDA组(p <；0.01和p <；0.05)。背外侧纹状体和黑质（SN）中酪氨酸羟化酶（TH）阳性的神经元和纤维较多(p <；0.05)。EA15组SN中TH的表达高于6-OHDA组(p <；0.05)， 6-OHDA组SN中α-synuclein的表达强于EA15组(p <；0.05)。EA15组纹状体左旋多巴水平高于6-OHDA组(p <；0.05)。结论6-羟多巴胺诱导的帕金森病大鼠在运动、认知行为和神经保护方面可能受益于耳穴电刺激。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.