Journal of Traditional and Complementary Medicine最新文献

{"title":"Rosmarinic acid Ameliorates neuronal regeneration in the bridging silicone rubber conduits of the sciatic nerve in taxol‐treated rats","authors":"Ping-Ling Chiu ,&nbsp;Mei-Chen Lin ,&nbsp;Shih-Tien Hsu ,&nbsp;Tin-Yun Ho ,&nbsp;Yung-Hsiang Chen ,&nbsp;Chung-Chia Chen ,&nbsp;Yueh-Sheng Chen","doi":"10.1016/j.jtcme.2024.03.004","DOIUrl":"10.1016/j.jtcme.2024.03.004","url":null,"abstract":"<div><h3>Background and aim</h3><p>Taxol modulates local inflammatory conditions in peripheral nerves, which may impair their regeneration and recovery when injured. This study aimed to determine the effects of rosmarinic acid (RA, a polyphenol constituent of many culinary herbs) on the regeneration of the sciatic nerves in the bridging conduits.</p></div><div><h3>Experimental procedure</h3><p>In the cell study, RA decreased nuclear factor (NF)-κB activity induced by taxol in a dose dependency. In the animal model, taxol-treated rats were divided into 3 groups (n = 10/group): taxol (2 mg/kg body weight for 4 times) and taxol + RA (3 times/week for 4 weeks at 20 and 40 mg/kg body weight) groups. Macrophage infiltration, calcitonin gene-related peptide (CGRP) expression levels, neuronal connectivity, animal behavior, and neuronal electrophysiology were evaluated.</p></div><div><h3>Results and conclusion</h3><p>At the end of 4 weeks, macrophage density, CGRP expression level, and axon number significantly increased in the RA group compared with the taxol group. The RA administration unaffected heat, cold plate licking latencies, and motor coordination. Moreover, the 40 mg/kg RA group had significantly larger nerve conduction velocity and less latency compared to the taxol group. This study suggested that RA could ameliorate local inflammatory conditions to augment the recovery of regenerating nerves by accelerating their regrowth and improving electrophysiological function in taxol-treated peripheral nerve injury repaired with the silicone rubber conduit.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 276-286"},"PeriodicalIF":4.5,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000233/pdfft?md5=27c07d371d6e6df3d4e50b5ceee1369b&pid=1-s2.0-S2225411024000233-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140083490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soybean oil induces neuroinflammatory response through brain-gut axis under high-fat diet","authors":"","doi":"10.1016/j.jtcme.2024.02.002","DOIUrl":"10.1016/j.jtcme.2024.02.002","url":null,"abstract":"<div><p>Neuroinflammation is considered the principal pathogenic mechanism underlying neurodegenerative diseases, and the incidence of brain disorders is closely linked to dietary fat consumption and intestinal health. To investigate this relationship, 60 8-week-old C57BL/6J mice were subjected to a 20-week dietary intervention, wherein they were fed lard and soybean oil, each at 15% and 35% fat energy. At a dietary fat energy level of 35%, inflammation was observed in both the soybean oil and lard groups. Nevertheless, inflammation was more pronounced in the mice that were administered soybean oil. The process by which nerve cell structure is compromised, inflammatory factors are upregulated, brain antioxidant capacity is diminished, and the TLR4/MyD88/NF-κB p65 inflammatory pathway is activated resulting in damage to the brain-gut barrier. This, in turn, leads to a reduction in the abundance of <em>Akkermansia</em> and <em>unclassified_f_Lachnospiraceae</em>, as well as an increase in <em>Dubosiella</em> abundance, ultimately resulting in brain inflammation and damage. These results suggested that soybean oil induces more severe neuroinflammation compared to lard. Our study demonstrated that, at a dietary fat energy level of 35%, compared to soybean oil, lard could be the healthier option, the outcomes would help provide a reference basis for the selection of residents’ daily dietary oil.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 5","pages":"Pages 522-533"},"PeriodicalIF":3.3,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000191/pdfft?md5=99c458f822e2ac0f63948cecd6c7f164&pid=1-s2.0-S2225411024000191-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140465354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bazi Bushen mitigates age-related muscular atrophy by alleviating cellular senescence of skeletal muscle","authors":"","doi":"10.1016/j.jtcme.2024.01.009","DOIUrl":"10.1016/j.jtcme.2024.01.009","url":null,"abstract":"<div><h3>Background and aim</h3><p>Muscular atrophy is one of the most common age-related conditions characterized by the deterioration of skeletal muscle structures and impaired functions. It is associated with cellular senescence and chronic inflammation, which impair the function of muscle stem cells. Bazi Bushen (BZBS) is a patent compound Chinese medicine that has been shown to have anti-aging effects in various animal models. In this study, we investigated the effects and mechanisms of BZBS on muscular atrophy in naturally aged mice.</p></div><div><h3>Experimental procedure</h3><p>A muscular atrophy model of naturally aged mice (18 months) was employed with administration of BZBS (2 g/kg/d, 1 g/kg/d) and nicotinamide mononucleotide (NMN, 200 mg/kg/d). After six months of drug administration, muscle weight loss, muscle function and muscle histopathology were measured to evaluate the therapeutic effect of BZBS. The expression of cellular senescence, inflammatory and satellite cell-related factors were used to assess the effects of BZBS in inhibiting cellular senescence, reducing inflammation and improving muscle atrophy.</p></div><div><h3>Results and conclusion</h3><p>Compared with age matched natural aging mice, we found that BZBS improved muscle strength, mass, and morphology by reducing senescent cells, inflammatory cytokines, and intermyofiber fibrosis in aged muscle tissues. We also found that BZBS prevented the reduction of Pax7 positive stem cells and stimulated the activation and differentiation into myocytes. Our results suggest that BZBS might be a promising intervention in senile muscular atrophy.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 5","pages":"Pages 510-521"},"PeriodicalIF":3.3,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000099/pdfft?md5=bf532ec2822075732006712c249ba855&pid=1-s2.0-S2225411024000099-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139875037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mulberry leaf extract inhibits obesity and protects against diethylnitrosamine-induced hepatocellular carcinoma in rats","authors":"Yun-Ching Chang ,&nbsp;Meng-Hsun Yu ,&nbsp;Hui-Pei Huang ,&nbsp;Dong-Hui Chen ,&nbsp;Mon-Yuan Yang ,&nbsp;Chau-Jong Wang","doi":"10.1016/j.jtcme.2024.01.007","DOIUrl":"10.1016/j.jtcme.2024.01.007","url":null,"abstract":"<div><p>Mulberry leaf has been recognized as a traditional Chinese medicinal plant, which was distributed throughout the Asia. The aqueous extract of mulberry leaf extract (MLE) has various biologically active components such as polyphenols and flavonoids. However, the inhibitory effect of MLE in hepatocarcinogenesis is poorly understood. In this study, we determined the role of MLE supplementation in preventing hepatocarcinogenesis in a carcinogen-initiated high-fat diet (HFD)-promoted Sprague-Dawley (SD) rat model. The rats were fed an HFD to induce obesity and spontaneous hepatomas by administering 0.01% diethylnitrosamine (DEN) in their drinking water for 12 weeks (HD group), and also to fed MLE through oral ingestion at daily doses of 0.5%, 1%, or 2%. At the end of the 12-week experimental period, the liver tumors were analyzed to identify markers of oxidative stress and antioxidant enzyme activities, and their serum was analyzed to determine their nutritional status and liver function. Histopathological analysis revealed that MLE supplementation significantly suppressed the severity and incidence of hepatic tumors. Furthermore, compared with the HFD + DEN groups, the expression of protein kinase C (PKC)-α and Rac family small GTPase 1 (Rac1) was lower in the MLE groups. These findings suggest that MLE prevents obesity-enhanced, carcinogen-induced hepatocellular carcinoma development, potentially through the protein kinase C (PKC)α/Rac1 signaling pathway. MLE might be an effective chemoprevention modality for nonalcoholic fatty liver disease (NAFLD)/nonalcoholic steatohepatitis (NASH)-related hepatocarcinogenesis.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 266-275"},"PeriodicalIF":4.5,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000075/pdfft?md5=263666bb48fdb3aa146228c5fb152be9&pid=1-s2.0-S2225411024000075-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139640128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural 7,8-secolignans from Schisandra sphenanthera fruit potently inhibit SARS-CoV-2 3CLpro and inflammation","authors":"","doi":"10.1016/j.jtcme.2024.01.005","DOIUrl":"10.1016/j.jtcme.2024.01.005","url":null,"abstract":"<div><p>The coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), turned into a global pandemic, and there remains an urgent demand for specific/targeted drugs for the disease. The 3C-like protease (3CL^pro) is a promising target for developing anti-coronavirus drugs. <em>Schisandra sphenanthera</em> fruit is a well-known traditional Chinese medicine (TCM) with good antiviral activity. This study found that the ethanolic extract displayed a significant inhibitory effect against SARS-CoV-2 3CL^pro. Forty-four compounds were identified in this extract using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Combining molecular docking and <em>in vitro</em> experiments, we found that two epimeric 7,8-secolignans, rel-(1<em>S</em>,2<em>R</em>)-1-(3,4-dimethoxyphenyl)-2-methyl-3-oxobutyl-3,4-dimethoxybenzoate (<strong>2</strong>) and rel-(1<em>S</em>,2<em>S</em>)-1-(3,4-dimethoxyphenyl)-2-methyl-3-oxobutyl-3,4-dimethoxybenzoate (<strong>4)</strong>, potently inhibited 3CL^pro with IC₅₀ values of 4.88 ± 0.60 μM and 4.75 ± 0.34 μM, respectively. Moreover, <em>in vivo</em> and <em>in vitro</em> experiments indicated that compounds <strong>2</strong> and <strong>4</strong> were potent in regulating the inflammatory response and preventing lung injury. Our findings indicate that compounds <strong>2</strong> and <strong>4</strong> may emerge as promising SARS-CoV-2 inhibitors via 3CL^pro inhibition and anti-inflammatory mechanisms.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 5","pages":"Pages 501-509"},"PeriodicalIF":3.3,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000051/pdfft?md5=914963c5062bc3674328c441064b5621&pid=1-s2.0-S2225411024000051-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular study of the KCNJ11 gene and its correlation with Prakriti to preventing and managing type 2 diabetes","authors":"","doi":"10.1016/j.jtcme.2024.01.004","DOIUrl":"10.1016/j.jtcme.2024.01.004","url":null,"abstract":"<div><p>In Ayurveda, every individual is believed to possess a unique entity known as <em>Prakriti,</em> which distinguishes them from others physically, physiologically, and psychologically. This entity also determines an individual's response to a particular stimulus, and it is believed that such responses are not solely determined by genetics. The present research aims to validate the Ayurvedic concept of <em>Prakriti</em> from a modern molecular perspective to strengthen the personalized and precise treatment approach. A study was conducted to investigate the role of the KCNJ11gene in the susceptibility of individuals to type 2 diabetes mellitus (T2DM) with their metabolic status. The research involved allele mining on three major <em>Prakriti</em> groups - <em>Vata, Pitta</em>, and <em>Kapha -</em> in 112 patients with T2DM and 112 healthy individuals. The KCNJ11 gene, responsible for insulin secretion membrane pore formation, was analyzed to determine the susceptibility of different <em>Prakriti</em> types to T2DM. The MutPred tool predicted the molecular cause of disease-related amino acid substitution. According to the study, only <em>Pitta</em> and <em>Kapha Prakriti</em> were diagnosed with diabetes, while all three <em>Prakriti</em> types were present in the control group of healthy individuals. A protein model was prepared, and the changes resulting from mutations were observed for each group in their protein sequence, both as synonymous and non-synonymous mutations. Ultimately, these changes contributed to the manifestation of T2DM. Based on the findings, it appears that <em>Prakriti</em> groups may experience changes in protein function due to nonsynonymous mutations and differences in amino acids at the protein level.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 5","pages":"Pages 494-500"},"PeriodicalIF":3.3,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S222541102400004X/pdfft?md5=ff2558027762bc3465f9e76e0fb66b99&pid=1-s2.0-S222541102400004X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140523385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin in treatment of hematological cancers: Promises and challenges","authors":"Maliheh Entezari ,&nbsp;Armita Tayari ,&nbsp;Mahshid Deldar Abad Paskeh ,&nbsp;Simin Khorsand Kheirabad ,&nbsp;Sahar Naeemi ,&nbsp;Afshin Taheriazam ,&nbsp;Hossein Dehghani ,&nbsp;Shokooh Salimimoghadam ,&nbsp;Mehrdad Hashemi ,&nbsp;Sepideh Mirzaei ,&nbsp;Saeed Samarghandian","doi":"10.1016/j.jtcme.2023.10.004","DOIUrl":"10.1016/j.jtcme.2023.10.004","url":null,"abstract":"<div><p>Hematological cancers include leukemia, myeloma and lymphoma and up to 178.000 new cases are diagnosed with these tumors each year. Different kinds of treatment including radiotherapy, chemotherapy, immunotherapy and stem cell transplantation have been employed in the therapy of hematological cancers. However, they are still causing death among patients. On the other hand, curcumin as an anti-cancer agent for the suppression of human cancers has been introduced. The treatment of hematological cancers using curcumin has been followed. Curcumin diminishes viability and survival rate of leukemia, myeloma and lymphoma cells. Curcumin stimulates apoptosis and G2/M arrest to impair progression of tumor. Curcumin decreases levels of matrix metalloproteinases in suppressing cancer metastasis. A number of downstream targets including VEGF, Akt and STAT3 undergo suppression by curcumin in suppressing progression of hematological cancers. Curcumin stimulates DNA damage and reduces resistance of cancer cells to irradiation. Furthermore, curcumin causes drug sensitivity of hematological tumors, especially myeloma. For targeted delivery of curcumin and improving its pharmacokinetic and anti-cancer features, nanostructures containing curcumin and other anti-cancer agents have been developed.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 2","pages":"Pages 121-134"},"PeriodicalIF":4.5,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001116/pdfft?md5=36b06bea4a56b44b2e924690885ea89c&pid=1-s2.0-S2225411023001116-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139392481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico anti-viral assessment of phytoconstituents in a traditional (Siddha Medicine) polyherbal formulation – Targeting Mpro and pan-coronavirus post-fusion Spike protein","authors":"Sumit Kumar Mandal ,&nbsp;MD Muzaffar-Ur Rehman ,&nbsp;Ashish Katyal ,&nbsp;Kanishk Rajvanshi ,&nbsp;Manoj Kannan ,&nbsp;Mohit Garg ,&nbsp;Sankaranarayanan Murugesan ,&nbsp;P.R. Deepa","doi":"10.1016/j.jtcme.2023.07.004","DOIUrl":"10.1016/j.jtcme.2023.07.004","url":null,"abstract":"<div><h3>Background and aim</h3><p>Novel nature of the viral pathogen SARS-CoV-2 and the absence of standard drugs for treatment, have been a major challenge to combat this deadly infection. Natural products offer safe and effective remedy, for which traditional ethnic medicine can provide leads. An indigenous poly-herbal formulation, Kabasura Kudineer from Siddha system of medicine was evaluated here using a combination of computational approaches, to identify potential inhibitors against two anti-SARS-CoV-2 targets – post-fusion Spike protein (structural protein) and main protease (Mpro, non-structural protein).</p></div><div><h3>Experimental procedure</h3><p>We docked 32 phytochemicals from the poly-herbal formulation against viral post-fusion Spike glycoprotein and Mpro followed by molecular dynamics using Schrodinger software. Drug-likeness analysis was performed using machine learning (ML) approach and pkCSM.</p></div><div><h3>Results</h3><p>The binding affinity of the phytochemicals in Kabasura Kudineer revealed the following top-five bioactives: Quercetin &gt; Luteolin &gt; Chrysoeriol &gt; 5-Hydroxy-7,8-Dimethoxyflavone &gt; Scutellarein against Mpro target, and Gallic acid &gt; Piperlonguminine &gt; Chrysoeriol &gt; Elemol &gt; Piperine against post-fusion Spike protein target. Quercetin and Gallic acid exhibited binding stability in complexation with their respective viral-targets and favourable free energy change as revealed by the molecular dynamics simulations and MM-PBSA analysis. <em>In silico</em> predicted pharmacokinetic profiling of these ligands revealed appropriate drug-likeness properties.</p></div><div><h3>Conclusion</h3><p>These outcomes provide: (a) potential mechanism for the anti-viral efficacy of the indigenous Siddha formulation, targeting Mpro and post-fusion Spike protein (b) top bioactive lead-molecules that may be developed as natural product-based anti-viral pharmacotherapy and their pleiotropic protective effects may be leveraged to manage co-morbidities associated with COVID-19.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 1","pages":"Pages 55-69"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000809/pdfft?md5=b2b56e439f1e7184f71e102a2a8580e4&pid=1-s2.0-S2225411023000809-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46287800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative traditional Chinese medicine treatment for children with obstructive sleep apnea","authors":"Wan-Yu Lai ,&nbsp;Chang-Ching Wei ,&nbsp;Chien-Heng Lin ,&nbsp;Liang- Wen Hang ,&nbsp;Ying-Hsiu Shih ,&nbsp;Fen-Wei Huang ,&nbsp;Hung-Rong Yen","doi":"10.1016/j.jtcme.2023.08.002","DOIUrl":"10.1016/j.jtcme.2023.08.002","url":null,"abstract":"<div><h3>Purpose</h3><p>Obstructive sleep apnea (OSA) is a chronic disease that affects 1%–6% of children. Our study aims to explore the effectiveness and clinical characteristics of integrative Traditional Chinese Medicine (ITCM) for pediatric OSA.</p></div><div><h3>Materials and methods</h3><p>In this retrospective cohort study, we assessed differences of polysomnography (PSG) parameters and clinical characteristics between 2009 and 2020. Children &lt;12 years old diagnosed with OSA (n = 508) were included and were categorized into ITCM cohort, western medicine (WM) cohort ,and surgery cohort. Outcomes were apnea-hypopnea index (AHI), respiratory disturbance index (RDI), and body mass index (BMI).</p></div><div><h3>Results</h3><p>There were 56 (11%), 324 (63.8%), and 128 (25.2%) patients in the ITCM, WM, and surgery cohorts. Among 17, 26, and 33 patients in the ITCM, WM, and surgery cohorts underwent follow-up PSG studies, respectively. In the ITCM follow-up cohort, AHI were significantly reduced (9.59 to 5.71, p &lt; 0.05). BMI significantly increased in the WM follow-up cohort (19.46 to 20.50, p &lt; 0.05) and the surgery follow-up cohort (18.04 to 18.85, p &lt; 0.01). Comparing ITCM to WM cohort, a significant difference was found between the changes in RDI (ITCM: −6.78, WM: 0.51, p &lt; 0.05) after treatment. Among ITCM follow-up cohort, the most prescribed TCM formula was Forsythia and Laminaria Combination. The most prescribed TCM herb was Ephedrae Herba.</p></div><div><h3>Conclusions</h3><p>ITCM therapy can significantly reduce RDI and control BMI. We identified potential TCM treatments for pediatric OSA. Further study of the pharmacological mechanisms and clinical efficacy is warranted.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 1","pages":"Pages 109-120"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000858/pdfft?md5=f7a182db60c7fd7bcfeba8bd506e2e94&pid=1-s2.0-S2225411023000858-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42386999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zhilong Huoxue Tongyu capsule alleviates myocardial fibrosis by improving endothelial cell dysfunction","authors":"Tao Bi ,&nbsp;Yanan Zhou ,&nbsp;Linshen Mao ,&nbsp;Pan Liang ,&nbsp;Jiali Liu ,&nbsp;Luyin Yang ,&nbsp;Guilin Ren ,&nbsp;Maryam Mazhar ,&nbsp;Hongping Shen ,&nbsp;Ping Liu ,&nbsp;Roman Spáčil ,&nbsp;Qing Guo ,&nbsp;Gang Luo ,&nbsp;Sijin Yang ,&nbsp;Wei Ren","doi":"10.1016/j.jtcme.2023.07.001","DOIUrl":"10.1016/j.jtcme.2023.07.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>Zhilong Huoxue Tongyu (ZL) capsule is a classical traditional Chinese medicine (TCM) with satisfactory curative effects. Endothelial cell (EC) dysfunction plays an important role during myocardial fibrosis (MF). But the therapeutic effect of ZL capsule on EC dysfunction remains unknown in the development of MF. This study aims to investigate the effect of ZL capsule on EC dysfunction during MF <em>in vivo</em>.</p></div><div><h3>Experimental procedure</h3><p>The model of MF is established <em>in vivo</em> by injecting isoproterenol for 14 days, simultaneously, we examined the therapeutic effect of ZL capsule on MF <em>in vivo</em>. An integrative approach combining biomarker examination, echocardiography and myocardial fibrosis condition using Hematoxylin-eosin staining, Masson staining, and Sirius red staining were performed to assess the efficacy of ZL capsule against MF. Subsequently, comprehensive immunofluorescence staining was performed to evaluate the therapeutic effect of ZL capsule on EC dysfunction.</p></div><div><h3>Results and conclusion</h3><p>Prior to experiments, analysis of the published single-cell sequencing data was performed and it was discovered that EC dysfunction plays an important role. Further pharmacological results showed that ZL capsule could alleviate fibrosis injury and collagen fiber deposition. The mechanism investigation results showed that the endothelial-to-mesenchymal transition (EndMT) and MHC class-II (MHC-II) expression in EC were improved. In addition, ZL capsule can attenuate the inflammatory response during MF by intervening the activation of CD4⁺T cell mediated by EC. For the first time, we provided evidence that ZL capsule could improve MF by alleviating EC dysfunction via the regulation of EndMT and expression of MHC-II.</p></div><div><h3>Taxonomy (classification by evise)</h3><p>Myocardial fibrosis, Chinese Herbal Medicine, Traditional Medicine, Endothelium, dysfunction, Endothelial-to-mesenchymal transition.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 1","pages":"Pages 40-54"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000767/pdfft?md5=bf9f9f9739dc0336dec8ea2eb8c47af3&pid=1-s2.0-S2225411023000767-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49142563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}