Tianqi pingchan granule promotes recovery of glymphatic system function in a rat model of l-DOPA-induced dyskinesia

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2024-04-29 DOI:10.1016/j.jtcme.2024.04.010

Zhihua Liu , Shuyuan Yang , Lu Song, Yu Zhang, Ying Wan, Jing Gan, Na Wu, Zhenguo Liu

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引用次数: 0

Abstract

Background and aim

Tianqi Pingchan Granule (TPG), as a traditional Chinese medicine, is used in the treatment of Parkinson's disease. l-DOPA-induced dyskinesia (LID) is mentioned as a debilitating motor complication in the course of Parkinson's disease. The purpose of this study is to investigate the effects of TPG on LID via the pathway of the glymphatic system.

Experimental procedure

A rat model of LID was used in this study. The severity of LID was assessed by behavioural tests. Three doses of TPG were administered once a day for 3 weeks to investigate the role of TPG in behavioural characteristics of LID rats. The glymphatic function was evaluated by utilizing intracisternal infusion of a fluorescenttracer. The amyloid-β levels were measured by Enzyme-linked immunosorbent assay. The expression and distribution of, and GFAP were assessed using immunofluorescence.

Results and conclusion

Dose-dependent effects of TPG in the 1behavioural characteristics were observed. Cortical and striatal influx cerebral spinal fluid tracer were reduced in LID rats with impaired AQP4 polarization. Amyloid-β, AQP4, and reactive astrogliosis was upregulated in LID rats. TPG administration increased cerebral influx of fluorescent tracer, restored polarized localization and expression of AQP4, and attenuated l-DOPA-induced amyloid-β deposits and astrogliosis. This study shows for the first time that glymphatic system function is impaired in LID and TPG attenuates abnormal involuntary movements in rats with LID by protecting the glymphatic system.

Section

1. Natural Products; 3. Dietary therapy supplements; 4. History, Philosophy and Social-Cultural aspects of Traditional Medicine.

Taxonomy (classification by evise)

identify the disease condition, the experimental approach.

Abstract Image

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天七平禅颗粒促进左旋多巴诱导运动障碍大鼠淋巴系统功能恢复

背景与目的：天气平禅颗粒是一种用于治疗帕金森病的中药。左旋多巴诱导的运动障碍（LID）被认为是帕金森病病程中的一种衰弱性运动并发症。本研究旨在探讨TPG通过淋巴系统通路对LID的影响。实验方法采用大鼠LID模型。通过行为测试评估LID的严重程度。每天1次给药3次TPG，连续3周观察TPG对LID大鼠行为特征的影响。通过腹腔内注射荧光示踪剂来评估淋巴功能。酶联免疫吸附法测定淀粉样蛋白-β水平。免疫荧光法检测GFAP的表达和分布。结果与结论TPG对大鼠的行为学有剂量依赖性。AQP4极化受损的LID大鼠脑脊液皮质和纹状体内流减少。淀粉样蛋白-β、AQP4和反应性星形胶质细胞增生在LID大鼠中上调。TPG增加了荧光示踪剂的脑内流，恢复了AQP4的极化定位和表达，减轻了l- dopa诱导的β淀粉样蛋白沉积和星形胶质细胞形成。本研究首次表明，LID大鼠的淋巴系统功能受损，TPG通过保护淋巴系统减轻了LID大鼠的异常不自主运动。天然产物;3. 膳食治疗补充剂；4. 传统医学的历史、哲学和社会文化方面。分类法（通过视觉分类）确定疾病状况，采用实验方法。

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.