Ameliorative effects of Sesamum Indicum aqueous extract on Letrozole-induced polycystic ovary syndrome in adult female rats and formulation of sesame syrup

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2024-04-16 DOI:10.1016/j.jtcme.2024.04.008

Zeynab Khosrowpour , Shamim Sahranavard , Fatemeh Jafari , Mojgan Tansaz , Shirin Fahimi , Mehrdad Faizi

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Abstract

Background

In this study, we explored the potential of Sesamum indicum L. aqueous extract (AES) in treating Polycystic ovary syndrome (PCOS) in female rats and developing a medicinal syrup from it.

Experimental procedure

We conducted experiments on 42 adult Sprague Dawley female rats, dividing them into six groups. The control group received a 1% carboxymethyl cellulose vehicle, while the other groups were given letrozole orally for 21 days followed by administration of AES, metformin, and distilled water after 28 days of PCOS induction.

Results

Results showed that after letrozole administration, PCOS was confirmed by irregular estrous cycles and increased LH and testosterone levels. The AES and metformin improved estrus cyclicity in rats compared to the PCOS group. Additionally, LH levels and LH/FSH ratio were significantly reduced in AES and metformin groups, while testosterone levels decreased and estradiol levels increased. Histological studies revealed normal follicular development in these groups with fewer cystic follicles.

Conclusion

In conclusion, the AES showed promise in alleviating PCOS symptoms due to its phytochemical components such as β-sitosterol, campesterol, and stigmasterol, and sesquiterpenes with phytoestrogenic, androgenic and antiinflammatory effects. The 400 mg/kg dose was found to be the most effective in this experiment.

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芝麻水提取物对来曲唑诱导的成年雌性大鼠多囊卵巢综合征的改善作用及芝麻糖浆配方

本研究探讨了芝麻水提物（AES）治疗雌性大鼠多囊卵巢综合征（PCOS）的潜力，并将其制成药用糖浆。实验方法将42只成年雌性大鼠分为6组。对照组给予1%羧甲基纤维素载体液，其余组给予来曲唑口服21 d，诱导PCOS 28 d后给予AES、二甲双胍和蒸馏水。结果来曲唑给药后，PCOS表现为月经周期不规则，LH和睾酮水平升高。与PCOS组相比，AES和二甲双胍改善了大鼠的发情周期。此外，AES组和二甲双胍组LH水平和LH/FSH比值显著降低，睾酮水平降低，雌二醇水平升高。组织学研究显示，这些组的卵泡发育正常，囊性卵泡较少。结论AES含有β-谷甾醇、油菜甾醇、豆甾醇等植物化学成分，以及具有植物雌激素、雄激素和抗炎作用的倍半萜类化合物，具有缓解PCOS症状的作用。本实验发现400 mg/kg的剂量最有效。

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.