Pei Lou Wong , Nur Khaleeda Zulaikha Zolkeflee , Nurul Shazini Ramli , Chin Ping Tan , Azrina Azlan , Faridah Abas
{"title":"通过常规评价和质子核磁共振（NMR）代谢组学分析药草蒿叶提取物的急性毒性特征","authors":"Pei Lou Wong , Nur Khaleeda Zulaikha Zolkeflee , Nurul Shazini Ramli , Chin Ping Tan , Azrina Azlan , Faridah Abas","doi":"10.1016/j.jtcme.2024.02.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and aim</h3><p>Interest in the safety of herbal medicine is growing rapidly regarding knowledge and challenges in natural products. Hence, this study aimed to reveal the toxicological profile of <em>Ardisia elliptica</em>, a traditional medicinal plant used in the treatment of various illnesses.</p></div><div><h3>Experimental procedure</h3><p>Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract, using a combination of conventional investigations and <sup>1</sup>H-NMR-based metabolomics approaches.</p></div><div><h3>Results</h3><p>Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. <sup>1</sup>H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, <sup>1</sup>H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.</p></div><div><h3>Conclusion</h3><p>This study suggested that 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000117/pdfft?md5=0cd1d2568e7879636a4f67485bf5bedf&pid=1-s2.0-S2225411024000117-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Acute toxicity profiling of medicinal herb Ardisia elliptica leaf extract by conventional evaluations and proton nuclear magnetic resonance (NMR) metabolomics\",\"authors\":\"Pei Lou Wong , Nur Khaleeda Zulaikha Zolkeflee , Nurul Shazini Ramli , Chin Ping Tan , Azrina Azlan , Faridah Abas\",\"doi\":\"10.1016/j.jtcme.2024.02.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background and aim</h3><p>Interest in the safety of herbal medicine is growing rapidly regarding knowledge and challenges in natural products. Hence, this study aimed to reveal the toxicological profile of <em>Ardisia elliptica</em>, a traditional medicinal plant used in the treatment of various illnesses.</p></div><div><h3>Experimental procedure</h3><p>Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract, using a combination of conventional investigations and <sup>1</sup>H-NMR-based metabolomics approaches.</p></div><div><h3>Results</h3><p>Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. <sup>1</sup>H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, <sup>1</sup>H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.</p></div><div><h3>Conclusion</h3><p>This study suggested that 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.</p></div>\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2225411024000117/pdfft?md5=0cd1d2568e7879636a4f67485bf5bedf&pid=1-s2.0-S2225411024000117-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2225411024000117\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2225411024000117","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Acute toxicity profiling of medicinal herb Ardisia elliptica leaf extract by conventional evaluations and proton nuclear magnetic resonance (NMR) metabolomics
Background and aim
Interest in the safety of herbal medicine is growing rapidly regarding knowledge and challenges in natural products. Hence, this study aimed to reveal the toxicological profile of Ardisia elliptica, a traditional medicinal plant used in the treatment of various illnesses.
Experimental procedure
Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic A. elliptica leaf extract, using a combination of conventional investigations and 1H-NMR-based metabolomics approaches.
Results
Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. 1H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, 1H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.
Conclusion
This study suggested that 2000 mg/kg BW of 70% ethanolic A. elliptica leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.
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