Sophorae tonkinensis radix polysaccharide attenuates acetaminophen-induced liver injury by regulating the miR-140-5p-related antioxidant mechanism

IF 3.3 3区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Liangliang Cai , Lixing Xu , Kai Shen , Qin Wang , Ronghua Ni , Xin Xu , Xiaofei Ma
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Abstract

STRP1, a polysaccharide active ingredient isolated from the traditional Chinese medicine Sophorae tonkinensis radix, has demonstrated a protective effect against acetaminophen (APAP)-induced liver injury (AILI). The underlying molecular mechanism was investigated in this study. Here, an acute liver damage mouse model was generated by APAP (400 mg/kg) and used to identify the protective effect of STRP1 (200 mg/kg) on mouse livers. In vitro cell experiments were used to further verify the related signaling pathways. Initially, in our study, STRP1 treatment reduced APAP-induced liver injury by decreasing aminotransferase activity and cell apoptosis and increasing cell proliferation. Furthermore, STRP1 treatment significantly increased Nrf2 expression and alleviated oxidative stress caused by reactive oxygen species in AILI. Based on bioinformatics and experimental studies, miR-140-5p was identified and found to be reduced by STRP1, increasing Nrf2 expression. Additionally, Nrf2 played an important role in the protective impact of STRP1-suppressed miR-140-5p expression. Generally, these results showed that STRP1-mediated suppression of miR-140-5p expression mitigates AILI by activating the Nrf2-mediated Nrf2-Keap1 pathway. This study revealed that STRP1 might be a potential treatment agent for AILI.

Abstract Image

刺槐多糖通过调节 miR-140-5p 相关抗氧化机制减轻对乙酰氨基酚诱导的肝损伤
STRP1 是一种从中药槐根中分离出来的多糖活性成分,对对乙酰氨基酚(APAP)诱导的肝损伤(AILI)具有保护作用。本研究对其潜在的分子机制进行了研究。本研究利用对乙酰氨基酚(APAP)(400 毫克/千克)建立了急性肝损伤小鼠模型,并利用该模型确定了 STRP1(200 毫克/千克)对小鼠肝脏的保护作用。体外细胞实验用于进一步验证相关信号通路。最初,在我们的研究中,STRP1 通过降低转氨酶活性和细胞凋亡以及增加细胞增殖,减轻了 APAP 引起的肝损伤。此外,STRP1 还能显著增加 Nrf2 的表达,减轻活性氧引起的 AILI 氧化应激。基于生物信息学和实验研究,发现 miR-140-5p 会被 STRP1 减少,从而增加 Nrf2 的表达。此外,Nrf2在STRP1抑制的miR-140-5p表达的保护作用中发挥了重要作用。总之,这些结果表明,STRP1 介导的 miR-140-5p 表达抑制可通过激活 Nrf2 介导的 Nrf2-Keap1 通路缓解 AILI。这项研究揭示了 STRP1 可能是一种潜在的 AILI 治疗药物。
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来源期刊
Journal of Traditional and Complementary Medicine
Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine
CiteScore
9.30
自引率
6.70%
发文量
78
审稿时长
66 days
期刊介绍: eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.
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