Neuroprotective effects of Tradescantia spathacea tea bioactives in Parkinson’s disease: In vivo proof-of-concept

IF 3.3 3区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Lorenna E.S. Lopes , Sheilla da Silva Barroso , Joanny K.M. Caldas , Paulo R. Vasconcelos , Kirley M. Canuto , Claudio Dariva , Klebson S. Santos , Patricia Severino , Juliana C. Cardoso , Eliana B. Souto , Margarete Z. Gomes
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引用次数: 0

Abstract

Background and aim

Tradescantia spathacea (T. spathacea) is a traditional medicinal plant from Central America and its tea, obtained by infusion, has been recognized as a functional food. The aim of this work was to investigate the effects of dry tea containing biocompounds from T. spathacea tea on motor and emotional behavior, as well as tyrosine hydroxylase (TH) and glial fibrillary acidic protein (GFAP) expression in 6-hydroxydopamine (6-OHDA)-lesioned rats.

Experimental procedure

Bioactives were identified by Ultra Performance Liquid Chromatography (UPLC) and an in vivo study in male Wistar rats was run as proof of concept of neuroprotective effects of DTTS.

Results and conclusion

We found 15 biocompounds that had not been previously reported in T. spathacea: the UPLC-QTOF-MS/MS allowed identification five phenolic acids, one coumarin, two flavonoids, one iridoid, one phenylpropanoid glycoside, and six fatty acid derivatives. The dry tea of T. spathacea (DTTS) presented significant antioxidant activity and high contents of phenolic compounds and flavonoids. Doses of 10, 30, and 100 mg/kg of DTTS were protective against dopaminergic neurodegeneration and exhibited modulatory action on the astrocyte-mediated neuroinflammatory response. Behavioral tests showed that 30 mg/kg of DTTS counteracted motor impairment, while 100 mg/kg produced an anxiolytic effect. The DTTS could be, therefore, a promising strategy for the management of Parkinson's disease.

Abstract Image

茶叶生物活性物质对帕金森病的神经保护作用:体内概念验证
背景和目的Tradescantia spathacea(T. spathacea)是中美洲的一种传统药用植物,通过浸泡获得的茶叶已被公认为一种功能性食品。这项工作的目的是研究含有刺五加茶生物化合物的干茶对 6-羟基多巴胺(6-OHDA)缺失大鼠的运动和情绪行为以及酪氨酸羟化酶(TH)和神经胶质纤维酸性蛋白(GFAP)表达的影响。实验过程通过超高效液相色谱法(UPLC)对生物活性物质进行鉴定,并对雄性 Wistar 大鼠进行体内研究,以证明 DTTS 具有神经保护作用的概念。刺五加干茶(DTTS)具有显著的抗氧化活性,酚类化合物和类黄酮含量高。剂量为 10、30 和 100 毫克/千克的 DTTS 对多巴胺能神经变性具有保护作用,并对星形胶质细胞介导的神经炎症反应具有调节作用。行为测试表明,每公斤 30 毫克的 DTTS 可抵消运动损伤,而每公斤 100 毫克的 DTTS 则可产生抗焦虑作用。因此,DTTS可能是治疗帕金森病的一种有前途的策略。
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来源期刊
Journal of Traditional and Complementary Medicine
Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine
CiteScore
9.30
自引率
6.70%
发文量
78
审稿时长
66 days
期刊介绍: eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.
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