Study on the pharmacodynamics and related mechanism of Tangningtongluo tablet on prediabetes mice based on the theory of “liver controlling dispersion”

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2024-06-28 DOI:10.1016/j.jtcme.2024.06.011

Xiangka Hu , Liuming Gui , Mushuang Qi , Wanjun Zhu , Ying Ren , Jin Li , Shuyu Yang , Chunmei Dai

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引用次数: 0

Abstract

Background and aim

Prediabetes is an unavoidable process and a high risk factor for developing of type 2 diabetes. Tangningtongluo (TNTL) tablet is a kind of pure plant preparation in hospital and used for the diabetes (消渴病, xiāo kě bìng) caused by deficiency of both Qi and Yin (气阴两虚，qì yīn liǎng xū). However, the effect of TNTL tablet on prediabetes has not been reported for now. This study was aimed to investigate the effect and related mechanism of TNTL tablet on prediabetes.

Experimental procedure

Prediabetic mice induced by a high-sugar and high-fat diet combined with streptozotocin were treated with TNTL tablet, and the pharmacodynamics and related mechanism were studied.

Results and conclusion

Our results showed that TNTL tablet reduced body weight and regulated the disorder of glucose and lipid metabolism in prediabetic mice. The function and the pathological changes of liver and islet tissue were improved after TNTL tablet treated prediabetic mice. Additionally, TNTL tablet decreased the levels of MDA, TNF-α, IL-1β and IL-6 in serum, increased the contents of SOD and GSH-px, up-regulated the expression of p-PI3K and p-AKT protein, and down-regulated the expression of p-IKK and p-NF-κB p65 protein in liver tissue. This study revealed that TNTL tablet could improve insulin sensitivity and insulin resistance in prediabetic mice, and delay the procession of prediabetes to diabetes, which might be related to inhibiting IKK/NF-κB signaling pathway, exerting anti-inflammatory and antioxidant effects, and activating PI3K/AKT signaling pathway.

Abstract Image

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基于“控肝散”理论的糖宁通络片对糖尿病前期小鼠的药效学及相关机制研究

背景与目的糖尿病前期是2型糖尿病不可避免的发病过程，是发生2型糖尿病的高危因素。糖宁通络片（TNTL）是一种医院用纯植物制剂，用于治疗气阴两虚（,qì n liǎng）引起的糖尿病（）。然而，TNTL片对前驱糖尿病的影响目前尚未见报道。本研究旨在探讨TNTL片对糖尿病前期的影响及其作用机制。实验步骤采用TNTL片治疗高糖高脂饮食联合链脲佐菌素诱导的糖尿病前期小鼠，研究其药效学及作用机制。结果与结论TNTL片对糖尿病前期小鼠具有减轻体重和调节糖脂代谢紊乱的作用。TNTL片对糖尿病前期小鼠的肝脏和胰岛组织功能及病理改变均有改善作用。此外，TNTL片降低血清MDA、TNF-α、IL-1β、IL-6水平，升高SOD、GSH-px含量，上调肝组织中p-PI3K、p-AKT蛋白表达，下调p-IKK、p-NF-κB p65蛋白表达。本研究发现，TNTL片可改善糖尿病前期小鼠胰岛素敏感性和胰岛素抵抗，延缓糖尿病前期向糖尿病的发展，其作用机制可能与抑制IKK/NF-κB信号通路，发挥抗炎、抗氧化作用，激活PI3K/AKT信号通路有关。

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.