白术和菟丝子提取物通过 PI3K/Akt 信号缓解糖尿病果蝇的胰岛素抵抗

IF 3.3 3区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Yinghong Li , Ye Xu , Biwei Zhang , Zhigang Wang , Leilei Ma , Longyu Sun , Xiuping Wang , Yimin Lin , Ji-an Li , Chenxi Wu
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引用次数: 0

摘要

背景和目的2型糖尿病(T2DM)的主要特征是由高血糖和胰岛素分泌不足引起的胰岛素抵抗(IR)。我们利用糖尿病蝇模型研究了白术和菟丝子提取物(AMK-CCL)对糖尿病蝇的作用及其分子机制。(实验方法采用高效液相色谱法测定白术提取物中的有效成分(鼠李糖、木糖、甘露糖和金丝桃苷)。将野生型果蝇(Cg-GAL4/+)或糖尿病果蝇(Cg > InRK1409A)分为对照组或二甲双胍组以及AMK-CCL(0.0125, 0.025, 0.05, 0.1 g/ml)组。测量食物摄入量、血淋巴葡萄糖和三卤葡萄糖、蛋白质、体重、甘油三酯(TAG)和糖原,以评估糖脂代谢。使用荧光报告物[tGPH、果蝇叉头盒 O(dFoxO)-绿色荧光蛋白(GFP)、Glut1-GFP、2-NBDG]检测体内磷脂酰肌醇-3-激酶(PI3K)/Akt 信号。结果 AMK-CCL 提取物含有 0.038 % 鼠李糖、0.017 % 木糖、0.69 % 甘露糖和 0.039 % 金丝桃苷。0.0125 g/mL的AMK-CCL可明显抑制糖尿病蝇循环血糖的升高以及体重、TAG和糖原含量的降低。AMK-CCL改善了糖尿病蝇的PI3K活性、Akt磷酸化、Glut1/3表达和葡萄糖摄取,还挽救了糖尿病诱导的dFoxO核定位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Atractylodes macrocephala Koidz. and Cuscuta chinensis Lam. extract relieves insulin resistance via PI3K/Akt signalling in diabetic Drosophila

Atractylodes macrocephala Koidz. and Cuscuta chinensis Lam. extract relieves insulin resistance via PI3K/Akt signalling in diabetic Drosophila

Background and aim

Type-2 diabetes mellitus (T2DM) is mainly characterized by insulin resistance (IR) induced by hyperglycaemia and insufficient insulin secretion. We employed a diabetic fly model to examine the effect and molecular mechanism of Atractylodes macrocephala Koidz. and Cuscuta chinensis Lam. (AMK–CCL) extract as traditional Chinese medicine in treating IR and T2DM.

Experimental procedure

The contents of the active ingredients (rhamnose, xylose, mannose, and hyperoside) in AMK–CCL extract were determined by high-performance liquid chromatography. Wild-type (Cg-GAL4/+) or diabetic (Cg > InRK1409A) Drosophila flies were divided into the control group or metformin group and AMK–CCL (0.0125, 0.025, 0.05, 0.1 g/ml) groups. Food intake, haemolymph glucose and trehalose, protein, weight, triglycerides (TAG), and glycogen were measured to assess glycolipid metabolism. Phosphatidylinositol-3-kinase (PI3K)/Akt signalling was detected using fluorescent reporters [tGPH, Drosophila forkhead box O (dFoxO)–green fluorescent protein (GFP), Glut1–GFP, 2-NBDG] in vivo. Glut1/3 mRNA levels and Akt phosphorylation levels were detected by quantitative polymerase chain reaction and western blotting, respectively, in vitro.

Results

AMK–CCL extract contained 0.038 % rhamnose, 0.017 % xylose, 0.69 % mannose, and 0.039 % hyperoside. AMK–CCL at 0.0125 g/mL significantly suppressed the increase in circulating glucose, and the decrease in body weight, TAG, and glycogen contents of diabetic flies. AMK–CCL improved PI3K activity, Akt phosphorylation, Glut1/3 expression, and glucose uptake in diabetic flies, and also rescued diabetes-induced dFoxO nuclear localisation.

Conclusions

These findings indicate that AMK–CCL extract ameliorates IR-induced diabetes via the PI3K/Akt signalling pathway, providing an experimental basis for clinical treatment.

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来源期刊
Journal of Traditional and Complementary Medicine
Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine
CiteScore
9.30
自引率
6.70%
发文量
78
审稿时长
66 days
期刊介绍: eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.
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