Midori Yasuda, Kenichiro Yasutake, Daichi Oikawa, Madoka Saiki, Ai Mitsuta-Takeyama
{"title":"Hydrolyzed Polyphenols from Water Chestnut (Trapa japonica) Suppress Lipase Activity in vitro and Blood Triacylglycerols in Mice.","authors":"Midori Yasuda, Kenichiro Yasutake, Daichi Oikawa, Madoka Saiki, Ai Mitsuta-Takeyama","doi":"10.5650/jos.ess24196","DOIUrl":"https://doi.org/10.5650/jos.ess24196","url":null,"abstract":"<p><p>Water chestnut (Trapa japonica) is a thorny aquatic floating plant that contains hydrolyzable polyphenols (WCPs) such as eugeniin, 1,2,3,6-tetra-O-galloyl-β-d-glucopyranose (TGG), and trapain. Previous studies have revealed that WCPs have antioxidant properties and can suppress postprandial blood glucose elevation in mice and human participants. However, the functionality of WCPs in treating obesity and metabolic syndrome, which have become significant issues in recent years, remains unclear. Here, we aimed to determine the effects of WCPs on lipid metabolism. We initially investigated the effects of WCPs on pancreatic lipase in vitro. Among WCPs, eugeniin and trapain, which contain a hexahydroxybiphenyl group, particularly inhibited lipase activity. We also found that WCPs suppressed fat accumulation in mouse adipose progenitor 3T3-L1 cells. We compared the effects of WCPs on lipid metabolism among groups of mice (n=7 each) fed for four weeks without (standard control and high-fat (HF) diet controls) or with HFWP 0.5 or HFWP 1 supplemented with 0.5 and 1% WCPs, respectively. Subsequently, we examined lipid-related parameters in adipose tissue, liver, and blood. We found significantly lower plasma triacylglycerol content in the HFWP1 than in the HF group. These findings suggest that WCPs may help prevent the onset of obesity and metabolic syndrome.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 3","pages":"297-307"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saravanakumar Venkatachalam, Johnson Retnaraj Samuel Selvan Christyraj, Jackson Durairaj Selvan Christyraj, Ravichandran Subramaniam, Melinda Grace Rossan Mathews, Jenif Leo Anandharaj, Kesavamoorthy Venkatachalam, Kalishwaralal Kalimuthu, Beryl Vedha Yesudhason
{"title":"Exploring the Fluorescence Bioactive Compounds in the Coelomic Fluid of Earthworms: Insights into Their Structural, Spectroscopic, and Functional Properties for Biomedical Applications.","authors":"Saravanakumar Venkatachalam, Johnson Retnaraj Samuel Selvan Christyraj, Jackson Durairaj Selvan Christyraj, Ravichandran Subramaniam, Melinda Grace Rossan Mathews, Jenif Leo Anandharaj, Kesavamoorthy Venkatachalam, Kalishwaralal Kalimuthu, Beryl Vedha Yesudhason","doi":"10.5650/jos.ess24168","DOIUrl":"https://doi.org/10.5650/jos.ess24168","url":null,"abstract":"<p><p>Coelomic fluid of earthworms is a valuable source of novel bioactive compounds with therapeutic applications. To gain insight into the bioactive compounds in the coelomic fluid, this study used Perionyx excavatus, a tropical earthworm distinguished for its remarkable ability for regeneration. This study aimed to identify fluorescent bioactive compounds in the coelomic fluid of P. excavatus and to investigate these compounds structural and functional characteristics for potential use in biomedical applications. Fluorescent bioactive compounds present in the coelomic fluid are identified using Thin Layer Chromatography (TLC), UV-visible spectrophotometry, and Spectrofluorometry techniques. Two unknown groups of fluorophore, named CFA and CFB, were analyzed by studying their emission spectra. In addition, GC-MS and LC-MS analyses provides detailed list of bioactive compound present in the coelomic fluid, in which indole and arachidonic acid shown maximum excitation and thus chosen for further studies. Their functional characterization reveals antibacterial activity, cytotoxicity and in-vitro wound healing assays, respectively. Notably, both of them exhibit significant efficacy against Aeromonas hydrophila, Salmonella typhi and Staphylococcus aureus. However, indole shows poor activity against Pseudomonas aeruginosa, whereas arachidonic acid demonstrates effective activity. These findings imply that these bioactive fluorescent compounds may have significant therapeutic applications.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 2","pages":"203-220"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed A Abdel-Wahab, Abdallah M Elgorban, Ali H Bahkali
{"title":"Valorization of Macroalgal Hydrolysate for the Production of Lipids and DHA by Marine Microbes.","authors":"Mohamed A Abdel-Wahab, Abdallah M Elgorban, Ali H Bahkali","doi":"10.5650/jos.ess24069","DOIUrl":"https://doi.org/10.5650/jos.ess24069","url":null,"abstract":"<p><p>The present study aimed to explore the potential of macroalgal hydrolysate to serve as an economical substrate for the growth of the oleaginous microbes Aspergillus sp. SY-70, Rhizopus arrhizus SY-71 and Aurantiochytrium sp. YB-05 for lipid and DHA production under laboratory conditions. The macroalgal hydrolysate was used at three concentrations 20, 40 and 80 g/L as a sole carbon source or in combination with 10 g/L of either acetic acid, glycerol, glucose, or sugarcane molasses. Glucose was used as a positive control at four different concentrations: 10, 20, 40, and 80 g/L. Out of the 19 carbon sources tested for the three microbes, 80 g/L macroalgae + 10 g/L molasses was the best source for Aspergillus sp. SY-70 (27.4 g/L DW and 9.73 g/L lipid) and R. arrhizus SY-71 (49.76 g/L DW and 16.88 g/L lipid), whereas 20 g/L macroalgae + 10 g/L glucose afforded the best source for Aurantiochytrium sp. YB-05 (27.93 g/L DW and 11.07 g/L lipid). Among the 19 carbon sources used for the growth of Aurantiochytrium sp. YB-05, we determined the fatty acid profile of the best four carbon sources that gave the highest biomass and lipid percentage. Among the four sources, 20 g/L macroalgal hydrolysate + glucose gave the highest DHA percentage (2.31 g/L), followed by 80 g/L pure glucose (1.68), 80 g/L macroalgal hydrolysate + glycerol (1.64), and 40 g/L macroalgal hydrolysate + molasses (1.52). The three carbon sources can replace pure glucose for the lipid, DPA, and DHA production using Aurantiochytrium sp. YB-05. The results of the current study suggest that we could use macroalgal hydrolysate in combination with molasses or glucose for the production of single-cell oil.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 2","pages":"187-201"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Discovery and Synthesis of Conjugated Fatty Acids from Natural Products.","authors":"Taro Honma","doi":"10.5650/jos.ess24282","DOIUrl":"https://doi.org/10.5650/jos.ess24282","url":null,"abstract":"<p><p>Conjugated fatty acids are a promising ingredient for cancer prevention and treatment. Conjugated fatty acids are minor fatty acids that are rarely found in nature, although a wide variety of structures are known. In recent years, studies have been conducted to screen natural products containing conjugated fatty acids and to synthesize conjugated fatty acids using enzymes derived from natural products. As a result, it was found that the seed oils of Centranthus ruber and Valeriana officinalis, which belong to the Valerianaceae family, contain conjugated linolenic acid, which has a conjugated triene structure in the molecule. Furthermore, it was found that parinaric acid, a conjugated tetraenoic fatty acid, can be synthesized by adding α-linolenic acid to enzymes extracted from the brown alga Padina arborescens Holmes. These research results are expected to be useful in securing conjugated fatty acids in quantities that can withstand practical application. Recent studies have reported that the cytotoxic effect of conjugated fatty acids is due to a programmed cell death called \"ferroptosis\". Many anticancer drugs exhibit anticancer activity through DNA modification, cell cycle arrest, angiogenesis inhibition, and epidermal growth factor receptor inhibition. Conjugated fatty acids, however, induce cell death through a mechanism distinct from these mechanisms and are therefore expected to be effective against cancers resistant to currently used anticancer drugs. The results of these studies will help to promote research on the use of conjugated fatty acids to overcome intractable cancers in the future.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 3","pages":"241-249"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isam A Mohamed Ahmed, Fahad AlJuhaimi, Mehmet Musa Özcan, Nurhan Uslu, Emad Karrar
{"title":"The Role of Roasting on Changes in Oil Contents, Bioactive Properties, Polyphenol Contents and Fatty Acid Profiles of Turpentine (Pistacia terebinthus L.) Fruit and Oils.","authors":"Isam A Mohamed Ahmed, Fahad AlJuhaimi, Mehmet Musa Özcan, Nurhan Uslu, Emad Karrar","doi":"10.5650/jos.ess24206","DOIUrl":"https://doi.org/10.5650/jos.ess24206","url":null,"abstract":"<p><p>In this study, the fluctuations in the oil content, total phenol, total flavonoid, radical scavenging capacity, phenolic constituent profiles and fatty acids of turpentine fruits during roasting of turpentine fruits and oils in the oven and microwave treatments were revealed. Total phenolic amounts of turpentine fruit and oils varied between 153.57 (oven) and 197.86 mgGAE/100 g (control) to 17.68 (control) and 30.65 mg GAE/100 g (oven), respectively. Total flavonoid values of the turpentine fruit and oils were characterized to be between 370.36 (microwave) and 567.50 mg/100 g (control) to 89.64 (oven) and 227.50 mg/100 g (microwave), respectively. While quercetin values of the turpentine fruits change between 171.73 (oven) and 330.88 mg/100 g (control), rutin amounts of fruits were defined to be between 3.66 (oven) and 10.00 mg/100 g (control). Catechin amounts of the turpentine fruits roasted in oven and microwave were specified to be between 3.42 (microwave) and 13.69 mg/100 g (oven). Oleic and linoleic acid contents of the oils extracted from raw and roasted turpentine fruits were assessed to be between 50.19 (oven) and 51.30% (control) to 22.89 (control) and 23.39% (oven), respectively. As a result, the phenolic components of turpentine oils were generally higher than those of turpentine fruits.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 3","pages":"251-259"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Straightforward Synthesis of Pinocembrin.","authors":"Kento Iwai, Nagatoshi Nishiwaki","doi":"10.5650/jos.ess24245","DOIUrl":"https://doi.org/10.5650/jos.ess24245","url":null,"abstract":"<p><p>A simple synthetic method for pinocembrin from cinnamic acid and 1,3,5-trihydroxybenzene was provided. This method can be performed in one-pot two steps reaction using inexpensive chemical reagents, whereas conventional methods need multiple steps from somewhat expensive starting reagents. The experimental procedure is facilitated, that is, to a DMF solution of cinnamoyl chloride generated in situ, a solution of 1,3,5-trihydroxybenzene and AlCl <sub>3</sub> in DCE/PhNO <sub>2</sub> was added, and the resultant mixture was heated to afford pinocembrin. This method does not require protection of phenolic hydroxy groups.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 1","pages":"75-78"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tsuyoshi Kato, Kana Yamamoto, Sanae Naito, Kaori Yamada, Emiko Mizuguchi
{"title":"Absolute Quantification of Individual Phospholipids in Infant Formula using Phosphorus Nuclear Magnetic Resonance Spectroscopy.","authors":"Tsuyoshi Kato, Kana Yamamoto, Sanae Naito, Kaori Yamada, Emiko Mizuguchi","doi":"10.5650/jos.ess24232","DOIUrl":"10.5650/jos.ess24232","url":null,"abstract":"<p><p>To accurately quantify the phospholipids in infant formula by subclass, we developed an analytical method using phosphorus-31 nuclear magnetic resonance spectroscopy (<sup>31</sup>P NMR). We performed heated extraction method using a mixture of ethanol and water to extract phospholipids from infant formula and replace the highly toxic chloroform traditionally used for extraction. In the <sup>31</sup>P NMR measurement, we also avoided using chloroform by dispersing the extracts in surfactants with a strong affinity for phospholipids. Although polar lipids in milk are characterized by a high content of sphingomyelin, the separation of sphingomyelin and phosphatidylethanolamine signals was insufficient to accurately determine their signal areas. To overcome this issue, we applied and evaluated two different methods, integration and deconvolution, for calculating the signal areas. During method validation in a spiked recovery test, the deconvolution method gave a recovery rate closer to 100% than the integration method. The main phospholipid subclasses found in infant formula were phosphatidylcholine, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and sphingomyelin. However, when the formula contained soy lecithin, additional soy-derived phospholipids, such as phosphatidic acid, were detected. Using equipment with a phosphorus resonance frequency of 202 MHz and a measurement time of approximately 4 h, the quantification limit was 5 mg/100 g. The developed method will be useful for analysis of phospholipids in infant formula.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 4","pages":"361-375"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation and Structural Properties of Gel Coating Films Containing Lipophilized Nanocarbon Particles Functionalized with Thixotropic Chains.","authors":"Yuka Hasunuma, Shoma Sakamoto, Yui Yamagishi, Atsuhiro Fujimori","doi":"10.5650/jos.ess24166","DOIUrl":"https://doi.org/10.5650/jos.ess24166","url":null,"abstract":"<p><p>Gel coating films comprising nanodiamonds organo-modified with 12-hydroxystearic (12-OHC<sub>18</sub> ) and stearic acids were prepared and characterized. Because molecules with 12-OHC<sub>18</sub> groups can convert solvents into thixotropic gels, Gemini-type diamide derivatives with two 12-OHC<sub>18</sub> chains were also introduced as thixotropic additives into the gel coating films. Although the 12-OHC<sub>18</sub> -modified nanodiamonds did not lead to solvent gelation on their own, they displayed an affinity for the thixotropic additive molecules. The 12-OHC<sub>18</sub> -modified nanodiamonds were localized near the surface of the nanofibers formed by the Gemini-type diamide derivative in the solvent, and the thixotropic properties of the supramolecular gel were confirmed. Nanoparticle aggregation and nanofiber crystallinity were found to be suppressed by the effect of 12-OHC<sub>18</sub> modification in the gel coating films, making them suitable for cosmetic coating applications.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 1","pages":"107-121"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kazuki Tada, Yuriko Suganuma, Aya Yoshinaga-Kiriake, Shota Koishi, Kazuaki Yoshinaga
{"title":"Analysis of Total, Free, and Esterified Sterols and Triterpene Alcohols in Edible Oils using a Gas Chromatography-Flame Ionization Detector.","authors":"Kazuki Tada, Yuriko Suganuma, Aya Yoshinaga-Kiriake, Shota Koishi, Kazuaki Yoshinaga","doi":"10.5650/jos.ess24091","DOIUrl":"https://doi.org/10.5650/jos.ess24091","url":null,"abstract":"<p><p>Sterols and triterpene alcohols exist in free and esterified forms in edible oils. To date, only few studies have determined the content of free or esterified sterols and triterpene alcohols using gas chromatography-flame ionization detection (GC-FID). In this study, analytical conditions were optimized using free and esterified sterol standards. To analyze total sterol and triterpene alcohol (Method A), edible oil sample was saponified in the presence of an internal standard (IS, 5α-cholestan- 3β-ol). However, to analyze free sterol and triterpene alcohol (Method B), the sample was dissolved in hexane/ethyl acetate (90:10, v/v) in the presence of the IS, and the free sterol and triterpene alcohol fractions were isolated via silica gel chromatography. The fractions obtained from Methods A and B were derivatized separately as trimethylsilyl (TMS) ether and analyzed using GC-FID. The esterified sterol and triterpene alcohol contents of edible oil were calculated by subtracting the free sterol content (Method B) from the total content (Method A). The major sterols and triterpene alcohols in canola, soybean, rice bran oil, and lard were separated using a column coated with 5% diphenyl/95% dimethylpolysiloxane. The recovery rates of free sterols spiked into canola oil were 86-106% and 87-109%, while those of esterified sterols (sitosteryl palmitate) spiked into canola oil were 94-105% and -4-2% based on Methods A and B, respectively. The sterol and triterpene alcohol compositions of lard, canola, soybean, and rice bran oils were also determined using Methods A and B, and the results aligned well with those reported in the literature. Altogether, our methods can be successfully used to quantify the levels of sterols and triterpene alcohols in edible oils.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"74 2","pages":"147-155"},"PeriodicalIF":1.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}