Journal of oleo science最新文献

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The Essential Oils Obtained from (Lonicerae japonicae Flos) Flower Buds Could Affect the Deposition of Sunflower Oil under Common High Temperature Conditions and the Traditional Frying Process in Maye. 从日本忍冬花蕾中提取的精油可影响马耶地区常见高温条件和传统油炸工艺下葵花籽油的沉淀。
IF 1.6 4区 农林科学
Journal of oleo science Pub Date : 2024-08-01 Epub Date: 2024-07-17 DOI: 10.5650/jos.ess23242
Mengrui Qu, Yuchen Liu, Dongying Wang
{"title":"The Essential Oils Obtained from (Lonicerae japonicae Flos) Flower Buds Could Affect the Deposition of Sunflower Oil under Common High Temperature Conditions and the Traditional Frying Process in Maye.","authors":"Mengrui Qu, Yuchen Liu, Dongying Wang","doi":"10.5650/jos.ess23242","DOIUrl":"10.5650/jos.ess23242","url":null,"abstract":"<p><p>The essential oil extracted from the flower buds of Lonicerae japonicae (LJEO) was employed in the high-temperature (65℃) accelerated preservation of sunflower oil. In the present investigation, the addition of the essential oil at a concentration of 800 ppm significantly inhibited the decrease in the oxidative stability of sunflower oil. This positive effect was achieved by significantly hindering the reduction in acidity value (AV), peroxide value (PV), p-anisidine value (AnV), the total oxidation value (TOTOX) (p < 0.01), and the levels of thiobarbituric acid reactive substance (TBARS), the absorbance at 232/268 nm (K232/K268) and total polar compounds (TPC) (p < 0.01). Besides, it also significantly enhances the sensory attributes of Maye, including taste, flavor, and appearance, improving its overall acceptability through the addition of certain potential fragrance molecules (p < 0.01). Furthermore, one of the primary chemical compounds in LJEO, eugenol, has demonstrated significant natural antioxidant properties in the traditional deep-frying procedure for the product, Maye. Consequently, together with eugenol, the essential oil LJEO could be employed as a possible effective antioxidant for the typical long-term preservation and even the traditional deep-frying procedures, and developed as effective antioxidant extracted from plants for the whole food industry.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":" ","pages":"1113-1124"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extending the Lifetime of Frying Oil through Optimization of Fryer Cleaning. 通过优化油炸锅清洁延长煎炸油的使用寿命。
IF 1.6 4区 农林科学
Journal of oleo science Pub Date : 2024-08-01 Epub Date: 2024-07-17 DOI: 10.5650/jos.ess23240
Noboru Ohyagi, Yomi Watanabe, Yoshiko Sugita-Konishi, Takayuki Morita, Mariko Mochizuki
{"title":"Extending the Lifetime of Frying Oil through Optimization of Fryer Cleaning.","authors":"Noboru Ohyagi, Yomi Watanabe, Yoshiko Sugita-Konishi, Takayuki Morita, Mariko Mochizuki","doi":"10.5650/jos.ess23240","DOIUrl":"10.5650/jos.ess23240","url":null,"abstract":"<p><p>With increases in consumer demand for fried foods in Japan over the last several decades, the consumption of frying oil has also steadily increased. Fryers used in restaurants to cook large quantities of food are typically cleaned using neutral kitchen detergents at the end of the day after removing the oil from the tank. However, significant amounts of debris can remain in the fryer after cleaning, possibly accelerating oil deterioration and thus reducing the quality of the fried foods. In this study, debris obtained from fryer tanks used in actual restaurants was assessed using scanning electron microscopy-energy dispersive X-ray spectroscopy together with Fourier transform infrared spectroscopy, and were determined to comprise polymerized oil and carbonized organic matter. Experiments using artificially prepared debris confirmed that these materials increased the acid value (AV) of frying oil. Trials in two restaurants serving similar amounts of fried chicken, French fries and doughnuts examined the effects of cleaning the fryer with either an alkaline detergent or a neutral kitchen detergent on debris removal and oil life. The alkaline detergent was found to completely remove debris while the neutral detergent left significant amounts of debris. After cleaning, the fryers were operated with new oil as usual and the deterioration of this oil was monitored by assessing the color difference, AV, carbonyl value and peroxide value. These indices increased 1.3 to 2.0 times faster in the case that the neutral kitchen detergent was used, suggesting that cleaning fryer tanks with an alkaline detergent could contribute to extending the lifetime of frying oil, reducing food losses and thus achieving sustainable development goals.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":" ","pages":"1035-1043"},"PeriodicalIF":1.6,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accelerated Recombination of Lophyl Radicals in Micelles: Rapid Controlled Self-Assembly of Micelles Formed by Amphiphilic Lophine Dimers and Release of Solubilized Substance by Photoirradiation 胶束中 Lophyl Radicals 的加速重组:两亲性洛芬二聚体形成的胶束的快速受控自组装以及通过光照射释放溶解物质
IF 1.5 4区 农林科学
Journal of oleo science Pub Date : 2024-06-01 DOI: 10.5650/jos.ess24047
Masaaki Akamatsu
{"title":"Accelerated Recombination of Lophyl Radicals in Micelles: Rapid Controlled Self-Assembly of Micelles Formed by Amphiphilic Lophine Dimers and Release of Solubilized Substance by Photoirradiation","authors":"Masaaki Akamatsu","doi":"10.5650/jos.ess24047","DOIUrl":"https://doi.org/10.5650/jos.ess24047","url":null,"abstract":"</p><p>Controlling the morphology of molecular assemblies formed by surfactants by photoirradiation enables the controlled release of incorporated substances, which can be applied to delivery systems for drugs and active ingredients. On the other hand, conventional photoresponsive surfactants and molecular assemblies have a slow response speed, making it difficult to control their functions at the desired time. In this review, I discuss our recent progress in the accelerated control of functions of photoresponsive molecular assemblies by using lophine dimer as a photochromic compound. The lophine dimer derivative dissociates into a pair of lophyl radicals that upon ultraviolet (UV) light irradiation, and these radical species thermally recombine although the recombination reaction is extremely slow due to the diffusion of lophyl radicals. By using the confined inner space of micelles formed by surfactants, the recombination reaction was extremely accelerated. With UV light irradiation, rapid morphological changes in micelles, formed by amphiphilic lophine dimers were observed by using in situ small-angle neutron scattering (in situ SANS) system. Moreover, the rapid controlled release of calcein as a model drug was achieved by UV light irradiation using the photoresponsive micelles. This rapid system can realize the controlled release of drugs truly at the desired time, developing an efficient and precise drug delivery system (DDS). Furthermore, it can be applied in a wide range of fields such as release control of active ingredients, efficient heat exchange control, and actuating systems.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/6/73_ess24047/figure/73_ess24047.jpg\"/>\u0000<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"12 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141191281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis of Zwitterionic Phospholipid-Connected Silane Coupling Agents and Their Hybridization with Graphene Oxide 合成与石墨烯氧化物杂化的硅烷偶联剂及与磷脂连接的硅烷偶联剂
IF 1.5 4区 农林科学
Journal of oleo science Pub Date : 2024-06-01 DOI: 10.5650/jos.ess24044
Kanato Arita, Seina Yarimizu, Maiko Moriguchi, Tsuyoshi Inoue, Haruyasu Asahara
{"title":"Synthesis of Zwitterionic Phospholipid-Connected Silane Coupling Agents and Their Hybridization with Graphene Oxide","authors":"Kanato Arita, Seina Yarimizu, Maiko Moriguchi, Tsuyoshi Inoue, Haruyasu Asahara","doi":"10.5650/jos.ess24044","DOIUrl":"https://doi.org/10.5650/jos.ess24044","url":null,"abstract":"</p><p>The hybridization of lipids with graphene is expected to produce a promising, novel biomaterial. However, there are limited examples of the covalent introduction of lipid molecules, especially the immobilization of lipid molecules, onto graphene on a substrate. Therefore, we investigated the hybridization of a silane coupling agent having phospholipid moieties with graphene oxide on substrates prepared by photo-oxidation using chlorine dioxide. Three silane coupling agents with different carbon chain lengths (C4, C6, C8) were synthesized and phospholipid molecules were introduced onto graphene on a substrate. Phospholipid-immobilized graphene on a grid for TEM (transmission electron microscope) was used for EM analysis of proteins (glyceraldehyde 3-phosphate dehydrogenase and β-galactosidase), enabling the observation of sufficient particles compared to the conventional graphene grid.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/6/73_ess24044/figure/73_ess24044.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"17 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141191323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unsaponifiable Compounds and Phenols Content, Antioxidant and Antitrypsin Activities of Prunus persica Kernel Oil 柿子仁油的不皂化物和酚类含量、抗氧化性和抗胰蛋白酶活性
IF 1.5 4区 农林科学
Journal of oleo science Pub Date : 2024-06-01 DOI: 10.5650/jos.ess24027
Cyrine Landolsi, Olfa Ben Salem-Berrabah, Moncef Feki, Saoussem Harrabi, Farah Hosseinian
{"title":"Unsaponifiable Compounds and Phenols Content, Antioxidant and Antitrypsin Activities of Prunus persica Kernel Oil","authors":"Cyrine Landolsi, Olfa Ben Salem-Berrabah, Moncef Feki, Saoussem Harrabi, Farah Hosseinian","doi":"10.5650/jos.ess24027","DOIUrl":"https://doi.org/10.5650/jos.ess24027","url":null,"abstract":"</p><p>Although peach kernels are rich in oil, there is a lack of information about its chemical and biological properties. Therefore, the purpose of this study was to determine the lipid profile, antioxidant capacity, and trypsin inhibitory propriety of peach oil extracted from two varieties (sweet cap and O’Henry) cultivated in Tunisia. The investigated peach kernel oil contains significant amount of unsaponifiable (2.1±0.5-2.8±0.2% of oil) and phenolic compounds (45.8±0.92-74.6±1.3 mg GAE/g of oil). Its <i>n</i>-alkane profile was characterized by the predominance of tetracosane <i>n</i>-C24 (47.24%) followed by tricosane <i>n</i>-C23 (34.43%). An important total tocopherol content (1192.83±3.1 mg/kg oil) has been found in sweet cap cultivar. Although rich in polyphenols and tocopherols, the tested oil did not display an inhibitory effect on trypsin. However, all peach oil samples showed effective antioxidant capacity and the highest values (86.34±1.3% and 603.50±2.6 μmol TE/g oil for DPPH test and ORAC assay, respectively) were observed for sweet cap oil. Peach oil has an excellent potential for application in the food and pharmaceutical industries as source of naturally-occurring bioactive substances.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/6/73_ess24027/figure/73_ess24027.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"55 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141191224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioaccessibility of Glucosylceramide in Rice Based on the Cooking Condition and Cultivar 基于烹饪条件和栽培品种的大米中葡萄糖甘油酰胺的生物可及性
IF 1.5 4区 农林科学
Journal of oleo science Pub Date : 2024-06-01 DOI: 10.5650/jos.ess24057
Shinji Yamashita, Shun Tanaka, Teruo Miyazawa, Mikio Kinoshita
{"title":"Bioaccessibility of Glucosylceramide in Rice Based on the Cooking Condition and Cultivar","authors":"Shinji Yamashita, Shun Tanaka, Teruo Miyazawa, Mikio Kinoshita","doi":"10.5650/jos.ess24057","DOIUrl":"https://doi.org/10.5650/jos.ess24057","url":null,"abstract":"</p><p>Glucosylceramide (GlcCer), a major sphingolipid in plants, possesses various food functions, including improvement of intestinal impairments. This study evaluated rice cooking conditions and cultivars based on GlcCer levels transferred into the digestive juice using an <i>in vitro</i> digestion model to investigate the factors related to GlcCer availability. GlcCer levels transferred into the digestive juice were higher in rice gruel than in boiled rice. The GlcCer levels in the digestive juice of boiled rice varied based on the rice cultivar, whereas those in rice gruel had no difference. Thus, GlcCer in rice was not fully utilized via digestion. Further, bioaccessibility was related to the amylose ratio and added water content.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/6/73_ess24057/figure/73_ess24057.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"52 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141191321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Heat-Cooking with Edible Fats and Oils on the Levels of 3-Chloro-1, 2-Propanediol Fatty Acid Esters (3-MCPDEs), 2-Chloro-1, 3-Propanediol Fatty Acid Esters (2-MCPDEs) and Glycidyl Fatty Acid Esters (GEs) in Processed Foods. 用食用油脂加热烹饪对加工食品中 3-氯-1,2-丙二醇脂肪酸酯(3-MCPDEs)、2-氯-1,3-丙二醇脂肪酸酯(2-MCPDEs)和缩水甘油脂肪酸酯(GEs)含量的影响。
IF 1.5 4区 农林科学
Journal of oleo science Pub Date : 2024-06-01 Epub Date: 2024-05-27 DOI: 10.5650/jos.ess24025
Yasushi Endo, Toru Fukazawa, Wataru Inoue, Yasuhiko Shigematsu, Yutaka Itabashi, Toshiharu Nagai
{"title":"Effects of Heat-Cooking with Edible Fats and Oils on the Levels of 3-Chloro-1, 2-Propanediol Fatty Acid Esters (3-MCPDEs), 2-Chloro-1, 3-Propanediol Fatty Acid Esters (2-MCPDEs) and Glycidyl Fatty Acid Esters (GEs) in Processed Foods.","authors":"Yasushi Endo, Toru Fukazawa, Wataru Inoue, Yasuhiko Shigematsu, Yutaka Itabashi, Toshiharu Nagai","doi":"10.5650/jos.ess24025","DOIUrl":"10.5650/jos.ess24025","url":null,"abstract":"<p><p>This study investigated the effect of cooking on the levels of 3-chloro-1, 2-propanediol esters (3-MCPDEs), 2-chloro-1, 3-propanediol esters (2-MCPDEs) and glycidyl esters (GEs) in deep-fried rice cracker, fried potato, croquette, fish fillet, chicken fillet and cooking oils (rice bran oil and palm oil). The levels of 2-/3-MCPDE in rice cracker fried with rice bran oil and the used oil remained about the same, while the levels of GEs in them fell with frying time. The levels of 2-/3-MCPDEs in fried potato, croquette, fried fish and chicken cutlet fried with rice bran oil and palm oil respectively fell with frying time, while the level of GEs in them remained about the same. The levels of 2-/3-MCPDEs and GEs in fried rice cooked with rice bran oil were under the method limit of quantification. These results provide insights the cooking has no influence with the levels of 2-/3-MCPDEs and GEs in cooked foods.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":" ","pages":"875-885"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Concentration of Diynoic Acids in Bicellar Mixtures Derived from Those Phase Separation 二炔酸在那些相分离得到的双细胞混合物中的浓度
IF 1.5 4区 农林科学
Journal of oleo science Pub Date : 2024-06-01 DOI: 10.5650/jos.ess24006
Shogo Taguchi, Soh Hamanishi, Hiroshi Satone, Takuji Yamamoto
{"title":"Concentration of Diynoic Acids in Bicellar Mixtures Derived from Those Phase Separation","authors":"Shogo Taguchi, Soh Hamanishi, Hiroshi Satone, Takuji Yamamoto","doi":"10.5650/jos.ess24006","DOIUrl":"https://doi.org/10.5650/jos.ess24006","url":null,"abstract":"</p><p>Bicellar mixtures containing diacetylene molecules, such as diynoic acids, can be used as parent materials for functional membranes. A bicellar mixture consisting of a diynoic acid—10,12-tricosadiynoic acid (TCDA)—, a phospholipid—1,2-dimyristoyl-<i>sn</i>-glycero-3-phosphocholine (DMPC)—, and a detergent—3-[(3-cholamidopropyl) dimethylammonio]-2-hydroxypropanesulfonate (CHAPSO)—was evaluated for its morphology and packing of TCDA molecules in its bicellar mixture. A TCDA/DMPC vesicle was prepared at different molar ratios, TCDA/DMPC = 2/8, 5/5, and 8/2; a TCDA/DMPC/CHAPSO bicellar mixture was prepared by mixing a CHAPSO solution with a TCDA/DMPC vesicle solution as a detergent at different composition ratios, <i>x</i> <sub>TCDA/DMPC</sub> = [TCDA/DMPC]/([TCDA/DMPC]+[CHAPSO]), of 1.0, 0.70, 0.50, and 0.30. A DMPC molecule formed a bilayer membrane structure and was used to suppress its precipitation. The packing density of the TCDA/DMPC/CHAPSO bicellar mixtures was increased by mixing a CHAPSO molecule in <i>x</i> <sub>TCDA/DMPC</sub> = 1.0 to 0.70 or 0.50. A TEM image of a TCDA/DMPC/CHAPSO bicellar mixture showed many discoidal assemblies at <i>x</i> <sub>TCDA/DMPC</sub> = 0.5 of TCDA/DMPC = 5/5. Polymerization of the TCDA molecules in the bicellar mixture by UV light suggested an ordered arrangement of TCDA. Polymerization at <i>x</i> <sub>TCDA/DMPC</sub> = 0.70 and 0.50 correlated with improved packing density.</p>\u0000<p></p>\u0000<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jos/73/6/73_ess24006/figure/73_ess24006.jpg\"/>\u0000graphical abstract <span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":"12 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141191390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the Anticancer Properties of Sakuranin Flavanone in Human Oropharyngeal Squamous Carcinoma Cells by Studying Its Effects on Caspase-driven Apoptosis, Mitochondrial Membrane Potential (MMP) Loss, Cell Migratory and Invasiveness and m-TOR/PI3K/AKT Signalling Pathway. 通过研究樱宁黄烷酮对 Caspase 驱动的细胞凋亡、线粒体膜电位 (MMP) 损失、细胞迁移和侵袭性以及 m-TOR/PI3K/AKT 信号通路的影响,探索其在人口咽鳞状癌细胞中的抗癌特性
IF 1.6 4区 农林科学
Journal of oleo science Pub Date : 2024-06-01 Epub Date: 2024-05-27 DOI: 10.5650/jos.ess23257
Fanyong Kong, Jie Zhai, Yueyue Shi, Jiaqi Xu, Haiyang Li, Shiyuan Zhang, Boxuan Han, Qian Shi, Yunxia Li, Xixi Shen, Shizhi He
{"title":"Exploring the Anticancer Properties of Sakuranin Flavanone in Human Oropharyngeal Squamous Carcinoma Cells by Studying Its Effects on Caspase-driven Apoptosis, Mitochondrial Membrane Potential (MMP) Loss, Cell Migratory and Invasiveness and m-TOR/PI3K/AKT Signalling Pathway.","authors":"Fanyong Kong, Jie Zhai, Yueyue Shi, Jiaqi Xu, Haiyang Li, Shiyuan Zhang, Boxuan Han, Qian Shi, Yunxia Li, Xixi Shen, Shizhi He","doi":"10.5650/jos.ess23257","DOIUrl":"10.5650/jos.ess23257","url":null,"abstract":"<p><p>Sakuranin is a flavanone which is a class of flavonoids found abundantly in Prunus species. Flavonoids have been long known for their anticancer properties against a range of human cancers. However, there are no previous reports on the anticancer effects of sakuranin flavanone molecule. This study was designed to study the anticancer effects of sakuranin against human oropharyngeal carcinoma cells along with investigating its effects on caspase-mediated apoptosis, mitochondrial membrane potential (MMP) loss, cell migration and invasion and m-TOR/PI3K/AKT signalling pathway. MTT assay was used to study effects on cell viability. The apoptotic studies were carried out through AO/EB staining, annexin V/FITC staining, comet assay and western blotting assay. Transwell chambers assay was used to study effects on cell migration and invasion. Flow cytometry was used to study effects of Sakuranin on mitochondrial membrane potential loss (MMP). Finally, western blotting was used to investigate m-TOR/PI3K/AKT signalling pathway. Results indicated that Sakuranin led to potent cell proliferation inhibition in a dose-dependent manner. Sakuranin also induced apoptotic cell death as indicated by fluorescence microscopy and annexin V/FITC staining assays. The apoptotic induction was mediated via activation of caspase-3, caspase-9, and Bax while as it led to downregulation of Bcl-2. Sakuranin also caused inhibition of cell migration and cell invasion along with causing significant decrease in MMP. Sakuranin also caused inhibition of expressions of proteins related with m-TOR/PI3K/AKT signalling pathway. In conclusion, the current findings clearly indicate anticancer effects of Sakuranin flavanone in human oropharyngeal cancer cells and are mediated via caspase activated apoptosis, inhibition of cell migration and invasion, loss of mitochondrial membrane potential and targeting m-TOR/PI3K/AKT signalling pathway.</p>","PeriodicalId":16626,"journal":{"name":"Journal of oleo science","volume":" ","pages":"911-920"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the Catabolic Rates of Linoleic and Oleic Acid Hydroperoxides Using 13CO2 Expired from Mice 利用小鼠体内排出的 13CO2 比较亚油酸和油酸氢过氧化物的分解率
IF 1.5 4区 农林科学
Journal of oleo science Pub Date : 2024-06-01 DOI: 10.5650/jos.ess23236
Aya Yoshinaga-Kiriake, Kazuaki Yoshinaga, Sae Miyagawa, Kanako Yoshino, Seiya Tanaka, Takumi Takahashi, Shunji Kato, Junya Ito, Yurika Otoki, Kiyotaka Nakagawa, Naohiro Gotoh
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