Journal of oral biology and craniofacial research最新文献

{"title":"Exploring plant-derived carbon dots: Dual-functional nanomaterials for endodontic infections and enhanced bio-imaging","authors":"Malavika Pradeep ,&nbsp;Anju Cecil ,&nbsp;Nidhita Suresh ,&nbsp;Rajakumar Govindasamy ,&nbsp;Natesan Thirumalaivasan","doi":"10.1016/j.jobcr.2025.08.030","DOIUrl":"10.1016/j.jobcr.2025.08.030","url":null,"abstract":"<div><h3>Background</h3><div>Endodontic infections are a major problem of public health, which requires new therapeutic and diagnostic approaches. Carbon dots (CDs) derived from natural sources have great potential as nanomaterials because of their exceptional properties, including biocompatibility and photoluminescence.</div></div><div><h3>Aim</h3><div>An investigation into the therapeutic possibilities of carbon dots derived from long pepper leaves(LCDs) for treating endodontic infections and parallel bio-imaging functionality forms the core research objective.</div></div><div><h3>Method</h3><div>LCDs through a hydrothermal process and checked their properties with FTIR, SEM-EDX, and fluorescence equipment. They tested the cells' acceptance of LCDs along with their resistance to bacteria and ability to enter cells using fibroblast growth tests, the <em>Streptococcus mutans</em> bacteria test, and microscope imaging.</div></div><div><h3>Results</h3><div>LCDs promoted better fibroblast development and endodontic infections healing above previous treatment standards. Biopsy tests show that LCD material kills <em>Streptococcus mutans</em> bacteria easily while also readily up taking cells for potential for Bio-imaging.</div></div><div><h3>Conclusion</h3><div>The study demonstrates that LCD types serve both as a medication for endodontic infections repair and a tool for living tissue observation. we have found a novel approach that will bring new possibilities to both managing endodontic infections and medical imaging techniques.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1408-1414"},"PeriodicalIF":0.0,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid two-stage CNN for detection and staging of periodontitis on panoramic radiographs","authors":"Rini Widyaningrum ,&nbsp;Eha Renwi Astuti ,&nbsp;Adioro Soetojo ,&nbsp;Amalia Nur Faadiya ,&nbsp;Aga Satria Nurrachman ,&nbsp;Netya Dzihni Kinanggit ,&nbsp;Abdul Harits Iftikar Nasution","doi":"10.1016/j.jobcr.2025.08.019","DOIUrl":"10.1016/j.jobcr.2025.08.019","url":null,"abstract":"<div><h3>Background</h3><div>Periodontal disease is an inflammatory condition causing chronic damage to the tooth-supporting connective tissues, leading to tooth loss in adults. Diagnosing periodontitis requires clinical and radiographic examinations, with panoramic radiographs crucial in identifying and assessing its severity and staging. Convolutional Neural Networks (CNNs), a deep learning method for visual data analysis, and Dense Convolutional Networks (DenseNet), which utilize direct feed-forward connections between layers, enable high-performance computer vision tasks with reduced computational demands. This study aims to evaluate the performance of a hybrid two-stage CNN integrating Mask R-CNN with DenseNet169 for detecting and staging periodontitis in panoramic radiographs.</div></div><div><h3>Methods</h3><div>A total of 600 panoramic radiographs were divided into training (70 %), validation (10 %), and testing (20 %) datasets, with an additional 100 external radiographs used as a final testing set. Four types of annotations were applied: tooth segmentation, radiographic bone loss (RBL), cementoenamel junction (CEJ) area, and periodontitis staging (normal, stage 1, 2, 3, and 4). Mask R-CNN was employed for segmentation training to detect teeth, CEJ, and RBL, while DenseNet169 served as the classifier for periodontitis staging.</div></div><div><h3>Results</h3><div>The hybrid two-stage CNN achieved a periodontitis staging performance on the external testing set with specificity and accuracy of 0.88 and 0.80, respectively.</div></div><div><h3>Conclusion</h3><div>These results demonstrate the potential of this hybrid two-stage CNN model as a diagnostic aid for periodontitis in panoramic radiographs. Further development of this approach could enhance its clinical applicability and accuracy.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1392-1399"},"PeriodicalIF":0.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro evaluation of color characterization, mechanical properties, and antimicrobial activity of 3D-printed provisional dental resin incorporated with Ficus carica (Fig) seed extract","authors":"Jeyaraj Brintha Jei,&nbsp;Balasubramaniam Muthukumar","doi":"10.1016/j.jobcr.2025.08.028","DOIUrl":"10.1016/j.jobcr.2025.08.028","url":null,"abstract":"<div><h3>Background of the study</h3><div><em>Ficus carica</em> known for its bioactive phytochemicals and antimicrobial potential; however, studies evaluating its outcome on the color, mechanical properties and antimicrobial activity of 3D-printed provisional dental resins are lacking. So this study evaluate the effect of <em>Ficus carica</em> seed extract incorporation on the color assessment, flexural strength, compressive strength, microhardness and antimicrobial activity of 3D-printed provisional crown and bridge resin.</div></div><div><h3>Materials and methods</h3><div>A total of 240 samples were prepared, with 60 samples allocated to four groups based on 0 %, 1.5 %, 2.5 %, and 5 % of <em>Ficus carica</em> seed extract incorporated into 3D provisional resin. Color analysis was evaluated using a spectrophotometer at baseline. Mechanical properties including flexural strength, compressive strength, and Vickers microhardness were assessed according to standardized protocols. Antimicrobial activity was tested against C albicans, S aureus, and P gingivalis by measuring zones of inhibition and calculating colony-forming units (CFU/mL). Data were analyzed using one-way ANOVA, followed by Tukey's HSD post hoc test. A p-value of &lt;0.05 was considered statistically significant.</div></div><div><h3>Results</h3><div>Color property improved, with ΔE decreasing from 0.5042 ± 0.186 (control) to 0.0700 ± 0.026 in the 5 % group, remaining within clinically acceptable limits. The Group D (5 %) showed significantly enhanced mechanical properties compared to the control (p &lt; 0.001). Mean compressive strength increased from 65.92 ± 1.02 MPa (control) to 76.09 ± 0.57 MPa in the 5 % group. Flexural strength improved from 55.77 ± 0.49 MPa to 69.53 ± 0.60 MPa, and microhardness increased from 11.17 ± 0.50 VHN to 14.02 ± 0.31 VHN. Antimicrobial activity demonstrated a dose-dependent increase, with the 5 % group showing the highest inhibition zones and greatest reduction in microbial counts across all tested organisms.</div></div><div><h3>Conclusion</h3><div><em>Ficus carica</em> seed extract incorporation especially at 5 % enhances the mechanical strength, color, and antimicrobial efficacy of 3D-printed provisional crown and bridge resin.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1400-1407"},"PeriodicalIF":0.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of hyaluronic acid filler on lip impressions: a cheiloscopic study","authors":"Kananda Loiola Fernandes de Aguiar ,&nbsp;Vandilson Pinheiro Rodrigues ,&nbsp;Rafael Soares Diniz ,&nbsp;Victor RM. Munoz-Lora ,&nbsp;José Ferreira Costa ,&nbsp;Letícia Machado Gonçalves-Soares","doi":"10.1016/j.jobcr.2025.08.025","DOIUrl":"10.1016/j.jobcr.2025.08.025","url":null,"abstract":"<div><h3>Objectives</h3><div>The objective of this study was to evaluate, through cheiloscopic analysis, the impact of hyaluronic acid (HA) lip augmentation on lip impressions. Eleven patients underwent HA injections and had their lips analyzed for thickness, morphological classification, commissure position, and lip impression types at three time points: before treatment (T1), one month after (T2), and three months after (T3). Lip thickness was measured using a digital caliper, and commissure position was classified based on standardized digital photographs. For lip impression recording, lipstick was applied, and the lips were then pressed against a substrate. The types of lip grooves were determined after dividing the impression into eight quadrants. Data were statistically analyzed using repeated measures ANOVA and Fisher's exact test, with a significance level of 5 %.</div></div><div><h3>Results</h3><div>There was a significant increase in both upper and lower lip thickness, which remained stable after 3 months (<em>p</em> &lt; 0,001). Regarding morphological classification, medium and mixed lips maintained their pattern in most cases, while thin lips became medium or mixed. No patient had “thick” or “very thick” lips after the treatment. There were also important changes in commissure position, particularly among patients with “lowered” commissures, which shifted to “horizontal.” No variations in lip impressions were observed across the quadrants examined at any time point.</div></div><div><h3>Conclusion</h3><div>The findings of this study suggest that, for purposes of human identification, the pattern of lip impressions remains unchanged after HA lip augmentation.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1378-1384"},"PeriodicalIF":0.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SND1, a novel m6A RNA regulator: Its high expression correlates with tumorigenesis and poor prognosis in head and neck squamous cell carcinoma","authors":"Karpakavinayakam Gopalakrishnan ,&nbsp;Chandra Pandi ,&nbsp;Vijayashree Priyadharsini Jayaseelan ,&nbsp;Paramasivam Arumugam","doi":"10.1016/j.jobcr.2025.08.024","DOIUrl":"10.1016/j.jobcr.2025.08.024","url":null,"abstract":"<div><h3>Background</h3><div>The multifunctional protein SND1 (Staphylococcal Nuclease and Tudor Domain Containing 1) is involved in transcriptional control, RNA metabolism, and tumour development. While its role in several cancer types has been studied, little is known about its importance in head and neck squamous cell carcinoma (HNSCC). This study investigates the expression patterns, clinical relevance, and functional role of SND1 in HNSCC.</div></div><div><h3>Methods</h3><div>SND1 expression in the TCGA-HNSCC dataset was examined using multiple databases, including TIMER, Protein Atlas, and UALCAN. Further SND1 expression was validated using real-time PCR. The prognostic impact of SND1 in HNSCC patients was analyzed using Kaplan-Meier survival analysis. Protein-protein interactions and pathway enrichment analyses were conducted to elucidate the SND1 functional role.</div></div><div><h3>Results</h3><div>The expression of SND1 was significantly elevated in HNSCC tissues compared to normal tissues. Higher grade, metastasis, TP53 alterations, HPV status, and advanced tumour stage were all substantially associated with elevated SND1 expression. According to Kaplan-Meier analysis, a lower overall survival rate was linked to SND1 high expression. Analysis of protein interactions identified partners such as AGO2, FXR1, and STAT5, which connect SND1 to carcinogenic pathways including JAK-STAT signalling. Functional enrichment pathways indicated SND1 role in oncogene translation regulation and head and neck carcinoma.</div></div><div><h3>Conclusion</h3><div>SND1 overexpression in HNSCC indicates a poor prognosis and unfavorable clinicopathological features. Due to its role in key cancer pathways, SND1 could serve as a useful biomarker for prognosis and a potential target for treatment. Improving patient outcomes requires further research into its molecular mechanisms and the development of treatments.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1385-1391"},"PeriodicalIF":0.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin-infused carboxymethyl cellulose–gelatin gel for oral tissue repair: Preliminary evaluation","authors":"Senthil Rethinam","doi":"10.1016/j.jobcr.2025.08.023","DOIUrl":"10.1016/j.jobcr.2025.08.023","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to develop and evaluate a multifunctional oral wound care gel (OWCG) formulated with carboxymethyl cellulose (CMC), gelatin (GEL), and curcumin (CUR) to enhance oral soft tissue regeneration.</div></div><div><h3>Methods</h3><div>CMC was synthesized through etherification, whereas CUR was extracted using a modified ethanol-based method. OWCG was prepared and crosslinked using 5 % citric acid. Physicochemical characterization including Fourier-transform infrared (FTIR) spectroscopy, high-resolution scanning electron microscopy (HR-SEM), thermogravimetric analysis (TGA), swelling behavior, and in vitro drug release were assessed. Biological evaluations included in vitro anti-inflammatory, antibacterial, and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assays on fibroblast (3T3-L1) cell, and scratch wound healing assays.</div></div><div><h3>Results</h3><div>OWCG exhibited a maximum swelling capacity of 86 % at neutral pH and 83 % CUR release under alkaline conditions within 24 h. In vitro protein denaturation inhibition was 82.4 %, confirming its anti-inflammatory efficacy. The gel exhibited notable antibacterial activity. MTT assays demonstrated over 90 % cell viability at 48 h, and scratch wound assays showed 90 % wound closure within the same timeframe.</div></div><div><h3>Conclusion</h3><div>OWCG demonstrated good biocompatibility, antimicrobial efficacy, and wound healing potential, supporting its use in oral wound care.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1367-1372"},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfiredoxin levels in gingival crevicular fluid: A new antioxidant defence system- case control study","authors":"Disha Dixit ,&nbsp;Shalini Kaushal ,&nbsp;Nand Lal ,&nbsp;Ranjana Singh ,&nbsp;Umesh Pratap Verma ,&nbsp;Anjani Kumar Pathak","doi":"10.1016/j.jobcr.2025.08.020","DOIUrl":"10.1016/j.jobcr.2025.08.020","url":null,"abstract":"<div><h3>Introduction</h3><div>Periodontitis is a chronic inflammatory condition in which oxidative stress plays a major role in its pathogenesis. The redox balance in the body acts as a defense system to counter this stress. The Peroxiredoxin (Prx)– Sulfiredoxin (Srx) antioxidant system is one such mechanism, found to be active in many systemic inflammatory diseases, including periodontitis. This study aims to compare the levels of Srx in Gingival Crevicular Fluid (GCF) of healthy and periodontally compromised patients after Non-surgical Periodontal Therapy (NSPT).</div></div><div><h3>Methods</h3><div>A total of 40 participants were divided into two groups: Group I included systemically and periodontally healthy individuals (control group), while Group II included patients diagnosed with chronic periodontitis (case group). Both groups received NSPT and were reassessed after three months. Clinical parameters such as Plaque Index (PI), Gingival Index (GI), Probing Pocket Depth (PPD), and Clinical Attachment Loss (CAL) were recorded and GCF samples were collected to measure Srx levels using the Enzyme-Linked Immunosorbent Assay (ELISA) at baseline and after three months.</div></div><div><h3>Results</h3><div>Srx levels significantly reduced after therapy in both groups. In Group I, levels decreased from 0.505 ± 0.20 ng/ml to 0.301 ± 0.13 ng/ml (p = 0.005). In Group II, levels decreased from 0.532 ± 0.23 ng/ml to 0.366 ± 0.10 ng/ml (p = 0.004). All clinical parameters also showed significant improvement at three months.</div></div><div><h3>Conclusion</h3><div>Srx levels decreased significantly after NSPT, suggesting its role as a potential biochemical marker to evaluate treatment outcomes. Further studies with larger samples and longer follow-ups are needed to confirm its diagnostic and prognostic value in periodontal therapy.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1373-1377"},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors influencing the retention rate of resin based pit and fissure sealant among primary school children- A randomised clinical trial","authors":"Aruna Vishwakarma ,&nbsp;Prashanth Vishwakarma ,&nbsp;Shruti Pundkar ,&nbsp;Anoli Agrawal","doi":"10.1016/j.jobcr.2025.08.010","DOIUrl":"10.1016/j.jobcr.2025.08.010","url":null,"abstract":"<div><h3>Background</h3><div>In modern dentistry, the focus is more on preventing caries than treating them, which helps preserve tooth structure. There is an approximately 50 % prevalence of caries on the occlusal surface in school children. Pits and fissures with irregularities and invasions are responsible for this. In dentistry, to overcome these problems, different sealant technique is introduced, for instance, the conventional method, using a bur, using air abrasion, etc. Therefore, this clinical trial was designed to evaluate the retention rate of sealant using different application techniques.</div></div><div><h3>Methodology</h3><div>The present Randomised clinical trial included 50 children aged 6–9 from a primary school children based on inclusion and exclusion criteria. The contralateral 1st mandibular molar teeth were divided randomly before placing sealant with two different techniques.</div></div><div><h3>Results</h3><div>The results obtained showed that there was a statistically highly significant difference in mean scores at the end of the 1st, 3rd, 9th, and 12th months of pit and fissure sealant application done with altered morphology without the use of bonding agent compared to altered morphology with bonding agent.</div></div><div><h3>Conclusion</h3><div>The present study showed that sealant retention was higher in the altered morphology without bonding agent group compared to the altered morphology with bonding agent.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1347-1353"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral health status, self-assessment and risk among tribes and narikuravars of Villupuramdistrict, Tamil Nadu – An epidemiological study","authors":"V. Kalaivani (Dr),&nbsp;Arthi Balasubramaniam (Dr),&nbsp;I. Meignana Arumugham (Dr)","doi":"10.1016/j.jobcr.2025.08.018","DOIUrl":"10.1016/j.jobcr.2025.08.018","url":null,"abstract":"<div><h3>Background</h3><div>Irular and Narikuravars, are the oldest Dravidian ethnic group and a semi-nomadic community people located in Tamil Nadu. Both the indigenous groups are relegated to the margins of society and face limited access to oral health care. Thus, we aimed to assess the normative need by assessing their oral health status and risks of Narikuravar and Irular tribes residing in Villupuram District, Tamil Nadu.</div></div><div><h3>Method</h3><div>ology: A cross-sectional epidemiological study with convenience sampling technique was conducted for 936 Irular and Narikuravar community people in Marakanam and Valavanur town panchayat of Dindivanam taluk of Villupuram district. Oral health status and self-assessment of oral health and risks was assessed using World Health Organization (WHO) oral health assessment form, self-oral health assessment form (2013).</div></div><div><h3>Result</h3><div>Narikuravar constitutes 32.8 % whereas Irula's constitute 67.2 % of the study population. Nearly 39.9 % of the study population had no formal schooling. Irular females with no formal schooling had high DMFT scores (3.27 ± 1.60). Also, the prevalence of gingivitis and periodontitis are high in Irular females with no formal schooling (32.3 % and 26 %) with no significant difference from Narikuravars. About 5.2 % of Narikuravar communities had high use of smoking and smokeless tobacco. Irulars consumed high cariogenic diet compared Narikuravars (p = 0.000).</div></div><div><h3>Conclusion</h3><div>Narikuvar community had more oral lesion compared to Irula's community people. Both communities had high prevalence of dental caries and periodontal disease. Females with no formal schooling of both communities had high caries experience, periodontitis.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1361-1366"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Friedelin induces apoptosis in oral cancer: Insights from in vitro and in silico studies","authors":"Ramya Sekar ,&nbsp;Monisha Prasad ,&nbsp;Manikandan Alagumuthu","doi":"10.1016/j.jobcr.2025.08.013","DOIUrl":"10.1016/j.jobcr.2025.08.013","url":null,"abstract":"<div><h3>Background</h3><div>Oral cancer is a major health issue in the world because of its high morbidity and mortality rates. Dysregulated apoptosis, is involved in tumor progression and treatment resistance. Friedelin, a natural triterpenoid, has been shown to have potential to modulate apoptosis pathways. This study investigates the therapeutic effects of Friedelin, particularly on its interactions with apoptotic proteins, cytotoxic effects on KB oral cancer cells, and ability to induce apoptosis through intrinsic signaling pathways.</div></div><div><h3>Materials and methods</h3><div>Interaction networks were constructed by taking target genes related to Friedelin and oral cancer identified by CTD and GeneCards, and applying them in the STITCH database for analysis. Apoptotic binding affinity of key proteins, towards Friedelin was determined using molecular docking. The cytotoxic potential of Friedelin was accessed by performing in vitro assays such as MTT, morphology analysis, and Annexin V-FITC flow cytometry. The regulation of Friedelin on apoptosis was validated through gene expression analysis.</div></div><div><h3>Results</h3><div>Network analysis identified Friedelin's critical interactions with apoptotic regulators. Molecular docking revealed strong binding affinities, particularly with Bax (−8.3 kcal/mol) and Bcl2 (−8.0 kcal/mol). Cytotoxicity assays showed dose- and time-dependent effects. Gene expression analysis confirmed upregulation of Bax, Caspase-3, and TP53, and downregulation of Bcl2, demonstrating activation of intrinsic apoptotic pathways.</div></div><div><h3>Conclusion</h3><div>Friedelin is an anticancer agent that has great potential for oral cancer treatment by modulating apoptotic signaling pathways and inducing apoptosis. Future studies should be conducted on the in vivo validation and clinical translation of Friedelin as a novel therapeutic candidate.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1354-1360"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}