Journal of oral biology and craniofacial research最新文献

{"title":"Tooth loss among adults with and without presence of systemic diseases – Age and Gender matched case control study","authors":"Deshpande Vijaya Lakshmi,&nbsp;S. Jagadeeswara Rao,&nbsp;Dolar Doshi,&nbsp;Twinkle Tiwari,&nbsp;Lunavath Preethi","doi":"10.1016/j.jobcr.2026.01.007","DOIUrl":"10.1016/j.jobcr.2026.01.007","url":null,"abstract":"<div><h3>Background</h3><div>Systemic diseases contribute to tooth loss by affecting immunity, circulation, and bone health. While most studies focus on elderly, this study assesses tooth loss among adults with and without systemic diseases to support early care.</div></div><div><h3>Aim</h3><div>To assess tooth loss among adults with and without systemic diseases in age- and gender-matched cases and controls.</div></div><div><h3>Design</h3><div>A case-control study was conducted among 280 adults (140 cases and 140 controls). Data on demographics, lifestyle factors (alcohol, smoking, oral hygiene), and systemic diseases were collected. Tooth loss was assessed using Tooth Loss Index. Statistical analysis included Chi-square, Mann-Whitney <em>U</em> test, logistic regression, and correlation analysis.</div></div><div><h3>Results</h3><div>Cases had higher rates of alcohol use, smoking, and poor oral hygiene (p &lt; 0.05). Tooth loss was significantly higher among cases (87.1 %) than controls (50 %), with greater mean missing teeth and higher Tooth Loss Index scores. Low education, alcohol, and smoking were significantly associated with increased tooth loss. Cases had nearly four times higher odds of significant tooth loss (OR = 3.82, p = 0.0001), with a strong positive correlation. (r = 0.82, p = 0.0001).</div></div><div><h3>Conclusion</h3><div>Systemic diseases are strongly associated with increased tooth loss in adults, highlighting need for early oral health interventions.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101403"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro mechanical performance of three CAD-CAM bar designs for implant-supported metal–resin hybrid prostheses: a preliminary pilot study","authors":"Arteaga-Losada Lorena ,&nbsp;Laura-Fernadez Héctor ,&nbsp;Puerta-Dominguez María Alejandra ,&nbsp;Ascaso-Terren Carlos ,&nbsp;Vives Eduard ,&nbsp;Escuin-Henar Tomás ,&nbsp;Torné-Durán Sergi","doi":"10.1016/j.jobcr.2026.101407","DOIUrl":"10.1016/j.jobcr.2026.101407","url":null,"abstract":"<div><h3>Background</h3><div>Implant-supported hybrid metal–resin prostheses are widely used to rehabilitate edentulous patients. However, fractures of the veneering resin and screw complications remain common mechanical failures. Advances in CAD-CAM design and laser sintering technology may improve the structural integrity of these restorations.</div></div><div><h3>Aims</h3><div>To conduct a preliminary in-vitro evaluation of the fracture resistance of veneering resin in three CAD-CAM–designed bar configurations fabricated by laser sintering, describing their mechanical behavior and failure patterns under compressive stress.</div></div><div><h3>Methods</h3><div>Three bar designs (inverted T, L-shaped, and Ackerman circular) were digitally created and manufactured in cobalt–chromium using laser sintering. Each bar was veneered with autopolymerizing acrylic resin and subjected to compressive loading up to 1000 N at a 30° angle, in accordance with ISO 14801. Simultaneously, acoustic emission analysis was performed to detect microcracks and structural failures.</div></div><div><h3>Results</h3><div>No fractures of the veneering resin were observed. Mechanical failures occurred as deformation or fracture of prosthetic screws, beginning at 600 N. Acoustic emission detected early microcracks between 160 N and 400 N, and main fracture peaks between 627 N and 871 N. Among the three samples, the inverted T-shaped bar sustained the highest load before failure in this pilot test.</div></div><div><h3>Conclusion</h3><div>In this pilot in-vitro study, the veneering resin showed high resistance under simulated masticatory loading. The combination of CAD-CAM design, laser-sintered fabrication, and retentive elements may enhance mechanical performance. Further studies with larger sample sizes and cyclic loading are warranted to validate these preliminary findings.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101407"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of colorimetry as a diagnostic tool for early detection of peri-implant diseases","authors":"Sergi Torné-Durán","doi":"10.1016/j.jobcr.2026.101406","DOIUrl":"10.1016/j.jobcr.2026.101406","url":null,"abstract":"<div><h3>Introduction</h3><div>The increasing aesthetic demand in dentistry and the limitations of visual colour assessment have encouraged the development of objective methods for evaluating dental and peri-implant tissues. Although colourimeters were originally designed for tooth shade matching, their application has recently expanded to peri-implant soft-tissue analysis, providing quantitative and reproducible measurements capable of detecting early inflammatory changes.</div></div><div><h3>Objectives</h3><div>This study aimed to determine whether colour analysis using a colourimeter is a valid diagnostic tool for the preliminary detection of peri-implant diseases by comparing colourimetric data with conventional clinical findings.</div></div><div><h3>Materials and methods</h3><div>A cross-sectional, descriptive, and experimental study was conducted on 63 dental implants. Peri-implant soft-tissue colour was recorded using a colourimeter based on CIELab parameters (L∗, a∗, b∗). Each implant also underwent clinical evaluation including visual inspection, periodontal probing, and periapical radiography. Two measurement points were analysed: Point A, located 2 mm apical to the gingival margin, and Point B, positioned beyond the probing depth for each implant. Colourimetric values from both points were compared with the clinical diagnosis obtained for every implant.</div></div><div><h3>Results</h3><div>At Point B, peri-implant tissues showed lower luminosity (L∗), higher redness (a∗), and reduced b∗ values compared with Point A. Clinically, 48 implants presented peri-implantitis, 9 mucositis, and 6 were considered healthy. Diseased implants demonstrated darker and more reddish peri-implant tissues, revealing a clear correlation between colour alterations and inflammatory status.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101406"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hybrid MMBERT framework for classifying periodontal bone loss: Integrating visual and textual information","authors":"Jennifer Jeyaruby,&nbsp;Pradeep Kumar Yadalam","doi":"10.1016/j.jobcr.2025.12.013","DOIUrl":"10.1016/j.jobcr.2025.12.013","url":null,"abstract":"<div><h3>Introduction</h3><div>Periodontitis, a chronic inflammatory disease, causes bone loss and tooth instability. Early diagnosis, aided by clinical and radiographic assessments, is crucial. mmBERT, a multimodal deep learning approach, integrates image and text data to enhance bone loss prediction but faces challenges such as image variability and limited datasets. This study proposes a Hybrid mmBERT Framework to improve diagnostic accuracy in intraoral periapical (IOPA) radiographs.</div></div><div><h3>Methods</h3><div>A dataset of 150 IOPA images from Saveetha Dias includes expert-annotated interpretations of periodontal bone loss. Images are split into training and test sets. Clinical notes are preprocessed, normalized, and feature-extracted using ClinicalBERT. The mmBERT model fuses image and text embeddings into a 1024-dimensional space with a 0.3 dropout rate. Its architecture integrates a vision encoder (ResNet50), text encoder (ClinicalBERT), and a cross-modal transformer with 16 attention heads.</div></div><div><h3>Results</h3><div>The study shows strong overlap between predicted and ground-truth masks, good intersection-over-union performance, and acceptable boundary accuracy. The MMBERT model achieved final training and test accuracies of 99.11 % and 100 %, respectively, demonstrating its robustness and reliability in clinical applications.</div></div><div><h3>Conclusion</h3><div>The study presents a classification method for periodontal bone loss based on MMBERT architecture. It demonstrates promising radiographic interpretation performance while necessitating further clinical application research.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101395"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compressive and flexural strength of provisional crown materials fabricated by conventional and 3D printing techniques: An in vitro study","authors":"Nandini R.D.,&nbsp;Nirupama R,&nbsp;Chetan Hegde","doi":"10.1016/j.jobcr.2026.101405","DOIUrl":"10.1016/j.jobcr.2026.101405","url":null,"abstract":"<div><h3>Purpose</h3><div>To compare the mechanical properties of the new 3D Accuprint C&amp;B resin against both conventional and other 3D-printed resins to determine its viability for provisional crown fabrication.</div></div><div><h3>Materials and methods</h3><div>Three materials were selected for comparison: GC Tempron (conventional), 3D Accuprint C&amp;B, and Freeprint Temp (3D-printed resins). Fifteen cylindrical specimens (6 mm × 4 mm) were prepared from each material for compressive strength testing, and fifteen rectangular specimens (25 mm × 2 mm × 2 mm) were fabricated for flexural strength testing as per the manufacturer's instructions. Testing was performed using a universal testing machine. The recorded compressive and flexural strength values were statistically analyzed after confirming normality (Shapiro–Wilk test) and homogeneity (Levene's test). All statistical analyses were conducted using IBM SPSS Statistics software (version 25), with the level of significance set at p &lt; 0.05. Data were analyzed using one-way ANOVA with Tukey's HSD test.</div></div><div><h3>Results</h3><div>Significant intergroup differences were found for both compressive and flexural strength (p &lt; 0.001). GC Tempron showed significantly lower values compared with both 3D printed materials (p &lt; 0.001). Freeprint Temp and 3D Accuprint did not differ significantly (compressive strength, p = 0.84; flexural strength, p = 0.15).</div></div><div><h3>Conclusions</h3><div>3D Accuprint C&amp;B demonstrated superior mechanical strength compared to GC Tempron and comparable properties to Freeprint Temp. These findings confirm its mechanical viability as a high-performance alternative, making it better suited for durable provisional restorations, particularly in cases involving high resistance to stress, parafunctional habits, or implant-supported prostheses.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101405"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High UBE2D1 expression is associated with poor prognosis and immunotherapy resistance in head and neck cancer","authors":"Prathibha Prasad ,&nbsp;Paramasivam Arumugam","doi":"10.1016/j.jobcr.2026.101423","DOIUrl":"10.1016/j.jobcr.2026.101423","url":null,"abstract":"<div><h3>Background</h3><div>Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy with poor clinical outcomes. Ubiquitin-conjugating enzyme E2 D1 (UBE2D1), a key component of the ubiquitination machinery, has been implicated in tumor progression in several cancers; however, its relevance in HNSCC remains unclear. This study aimed to investigate UBE2D1 expression in HNSCC, evaluate its prognostic significance while acknowledging disease heterogeneity, including HPV and TP53 status, and explore its potential association with response to anti-PD-1 immunotherapy.</div></div><div><h3>Methods</h3><div>UBE2D1 mRNA and protein expression were analyzed using the Cancer Genome Atlas Head and Neck Squamous Cell Carcinoma (TCGA-HNSCC) dataset via the UALCAN platform. Transcript-level expression was validated by quantitative real-time PCR in paired HNSCC and adjacent normal tissues. Protein–protein interaction and functional enrichment analyses were performed to examine UBE2D1-associated molecular networks. Prognostic relevance was assessed using Kaplan–Meier survival analysis.</div></div><div><h3>Results</h3><div>UBE2D1 mRNA and protein expression were significantly elevated in HNSCC compared with normal tissues (<em>P</em> &lt; 0.05). Higher UBE2D1 expression was associated with poorer overall and relapse-free survival. Exploratory analyses suggested a weak association between elevated UBE2D1 expression and response patterns to anti-PD-1 therapy. Functional analyses identified associations with oncogenic pathways, with UBE2D1 expression positively correlated with MMP2 and negatively correlated with SMAD4, indicating a potential role in tumor progression.</div></div><div><h3>Conclusion</h3><div>UBE2D1 is frequently overexpressed in HNSCC and is associated with adverse clinical outcomes. Although its association with immunotherapy response requires further validation, UBE2D1 may be a prognostic biomarker in HNSCC.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101423"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of surface preparation methods on the shear bond strength of universal and self-etch adhesive systems: an in vitro study","authors":"Shiwangi Verma ,&nbsp;Ravishankar Suman ,&nbsp;Rajendra Goud ,&nbsp;Snigdho Das ,&nbsp;Srikant Gollapudi ,&nbsp;Samapika Routray","doi":"10.1016/j.jobcr.2026.101408","DOIUrl":"10.1016/j.jobcr.2026.101408","url":null,"abstract":"<div><h3>Objectives</h3><div>To assess the impact of three smear layer preparation methods on shear bond strength (SBS) to enamel and dentin, using two universal adhesives (Tetric N-Bond Universal, 3M Single Bond Universal) and one two-step self-etch adhesive (Kuraray Clearfil SE Bond).</div></div><div><h3>Methods</h3><div>Ninety extracted premolars were assigned to enamel and dentin groups (n = 45 each) and prepared with coarse grit (120), fine grit (600) silicon carbide paper, or air abrasion (Aluminium Oxide for enamel, Sylc for dentin). Surfaces were bonded with one of the adhesives and restored with composite. Enamel was treated in an etch-and-rinse mode, dentin in self-etch mode. SBS was tested using a universal testing machine. Failure surfaces were examined under SEM to assess fracture morphology and EDS to determine elemental composition. Data were analyzed using two-way ANOVA/Tukey's post-hoc test and Weibull statistics (P ≤ 0.05).</div></div><div><h3>Results</h3><div>In enamel, SBS generally increased from coarse to fine to air abrasion. Kuraray Clearfil SE Bond yielded the highest values, particularly with air abrasion (9.11 ± 0.51 MPa). In dentin, fine grit achieved the highest SBS across all adhesives, followed by air abrasion; coarse grit showed the lowest. Kuraray consistently outperformed the universal adhesives in most dentin conditions. SEM/EDS revealed predominantly adhesive failures; occasional cohesive failures in dentin (Ivoclar) and enamel (Kuraray) corresponded with higher SBS and Weibull reliability.</div></div><div><h3>Conclusion</h3><div>Both surface preparation and adhesive type significantly influence SBS. Kuraray showed superior performance and reliability, while fine grit and air abrasion enhanced bonding efficacy. SEM/EDS findings corroborated the mechanical results.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101408"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regional odontodysplasia: a report of unusual case with in-depth analysis","authors":"Charles Béhot ,&nbsp;Ophélie Cuisinier ,&nbsp;Éden Serraf ,&nbsp;Lisa Friedlander ,&nbsp;Brigitte Vi-Fane ,&nbsp;Pascal Garrec ,&nbsp;Ali Nassif","doi":"10.1016/j.jobcr.2025.12.012","DOIUrl":"10.1016/j.jobcr.2025.12.012","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Regional odontodysplasia (RO) is a rare developmental anomaly affecting dental tissue formation, typically confined to a single quadrant. It manifests through hypoplastic enamel, irregular dentin, short roots with open apices, and a characteristic “ghost-like” radiographic appearance. Its etiology remains unclear, with vascular, genetic and inflammatory factors proposed.</div></div><div><h3>Materials and methods</h3><div>In this study, we report the case of a 15-year-old female with mandibular RO affecting canine, premolars, and molars, focusing on the clinical, radiographic, and microscopic analysis of the affected teeth, including mineral density evaluation.</div></div><div><h3>Results</h3><div>The 2nd premolar and first and second molars (teeth 35, 36, 37) exhibited severe morphological defects and were extracted due to extreme root shortening (40 %, 90 %, and &gt;95 % reduction), while alveolar bone height remained preserved. The canine and 1st premolar were affected to a lesser extent and were conserved upon orthodontic treatment plan. Scanning electron microscopy revealed slight decreases in the number and diameter of the dentinal tubules in the affected teeth compared with those in the control teeth. Contrary to many reported cases of RO, mineral density analysis of enamel and dentin revealed no significant differences between affected and normal teeth. The observations reported in this case suggest that resorptive changes, possibly influenced by inflammatory factors, could contribute to the observed root anomalies. The preservation of alveolar bone height provides a favourable prognosis for prosthetic rehabilitation.</div></div><div><h3>Conclusion</h3><div>These findings help elucidate the structural and functional implications of ROs. This study highlights the importance of early diagnosis and multidisciplinary management for optimal outcomes.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101394"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dimethyl sulfoxide pretreatment on bonding of universal adhesives to pulp chamber dentin","authors":"Yashmin Parveen Karishma,&nbsp;Rajni Nagpal,&nbsp;Udai Pratap Singh,&nbsp;Sakshi Saumya,&nbsp;Shivani Deshwal","doi":"10.1016/j.jobcr.2026.101409","DOIUrl":"10.1016/j.jobcr.2026.101409","url":null,"abstract":"<div><h3>Aim and objective</h3><div>To evaluate the effect of 50 % (v/v) dimethyl sulfoxide (DMSO) pretreatment on immediate (24 h) and delayed (6 months) microtensile bond strength (μTBS) of an etch-and-rinse adhesive and two universal adhesives to dentin.</div></div><div><h3>Materials and methods</h3><div>A total of 144 extracted human molars were decoronated, pulp chambers exposed, and roots sectioned 2 mm apical to the CEJ. Teeth were randomly divided into six experimental groups (n = 24), based on surface pretreatment and adhesive system used: Group 1A: Adper Single Bond 2 (ASB) Group 2A: Tetric N-Bond Universal (TNBU) Group 3A: Prime &amp; Bond Universal (PNBU) Groups 1B, 2B, and 3B received the same adhesives, respectively, following a 50 % (v/v) DMSO wet bonding pretreatment. All adhesives were applied per manufacturer's instructions, followed by composite resin build-up. Ten samples per group were tested for μTBS at 24 h and ten after 6 months of storage. Failure mode was analyzed. Four additional samples per group underwent SEM evaluation to observe the resin–dentin interface.</div></div><div><h3>Statistical analysis</h3><div>Data were analyzed using one-way ANOVA and Tukey's post hoc test for intergroup comparisons. Unpaired t-tests were applied for two-group comparisons. Significance was set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>DMSO pretreatment significantly improved both immediate and long-term μTBS (p &lt; 0.05) across all adhesives. PNBU exhibited the highest bond strength at both time intervals.</div></div><div><h3>Conclusion</h3><div>Dentin pretreatment with 50 % DMSO prior to adhesive application enhances immediate μTBS and preserves bond durability at 6 months, regardless of adhesive system used.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101409"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential effect of Periodontal pathogen Fusobacterium nucleatum and Porphyromonas gingivalis on modulation of specific partial-EMT genes in colorectal cancer cells","authors":"Divya Gopinath ,&nbsp;Balachandar Selvakumar ,&nbsp;Priyadharshini Sekar ,&nbsp;Marwan Mansoor Mohammed ,&nbsp;Zhengrui Li","doi":"10.1016/j.jobcr.2026.101410","DOIUrl":"10.1016/j.jobcr.2026.101410","url":null,"abstract":"<div><h3>Objective</h3><div>While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of <em>Fusobacterium nucleatum</em> (Fn) and <em>Porphyromonas gingivalis</em> (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).</div></div><div><h3>Materials and methods</h3><div>HCT 116 cells were infected with <em>Fn</em> or <em>Pg</em> at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate prognostic significance.</div></div><div><h3>Results</h3><div><em>Pg</em> infection induced a significant upregulation of EMP3 at the 24 h, while suppressing the expression of P4HA2, MXD1, and THBS2. The ZEB2 displayed a biphasic response, decreasing at 6 h before rising at 24 h, and SNAI1 was consistently suppressed. In contrast, <em>Fn.</em> infection resulted in the upregulation of ITGA5 and MMP3, accompanied by the downregulation of P4HA2, MXD1, MMP9, and SNAI1. Both enhanced cellular migration and wound closure capabilities. THBS2, CDH13, and P4HA2 were significantly elevated in tumor tissues compared to controls, while EMP3 and ZEB1 showed reduced expression. Elevated mRNA levels of EMP3, THBS2, and ITGA5 were significantly correlated with poorer overall survival.</div></div><div><h3>Conclusion</h3><div><em>Fn</em> and <em>Pg</em> can promote invasive phenotypes in CRC through distinct mechanisms, each modulating a unique subset of p-EMT and without instigating a complete, coordinated EMT gene signature.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101410"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}