Journal of oral biology and craniofacial research最新文献

{"title":"Evaluation of nanofiber scaffolds laden Ashvakatri in the management of chronic periodontitis-a randomized, controlled split pocket study","authors":"Pragati Dubey ,&nbsp;Neelam Mittal ,&nbsp;Brahmeshwar Mishra ,&nbsp;Anju Gautam ,&nbsp;Naveen Kumar ,&nbsp;M. Rashika","doi":"10.1016/j.jobcr.2025.07.027","DOIUrl":"10.1016/j.jobcr.2025.07.027","url":null,"abstract":"<div><h3>Background</h3><div>The main objective of periodontal treatment is to stop the progression of periodontal disease. Controlled-release drugs yield promising outcomes when conventional treatment is proven to be insufficient in establishing periodontal health in chronic periodontitis. A low-dose controlled-release delivery method for the treatment of periodontal infection was attempted to be developed in this study. With effective electrospinning, a novel sustained-release medication system including polycaprolactone (PCL) nanofibers containing <em>Ashvakatri</em> (A2) and Tetracycline (TET) was accessed clinically for periodontitis.</div></div><div><h3>Method &amp; materials</h3><div>The electrospinning technique prepared nanofibers with A2 and TET in PCL and gelatin. The A2-loaded nanofiber followed the Higuchi model release and had a sustained impact of 9 days (220 h). 75 periodontal sites from 31 patients with chronic periodontitis (≥5 mm probing depth) followed by 3 groups: Group I received Scaling and root planning (SRP) and blank polymer mat whereas Group II treated with SRP and PCL-GE-A2 nanofiber scaffold/mat, and Group III received SRP + PCL-GE-TET (tetracycline) nanofiber scaffolds. Clinical evaluations of GI, PI, PPD, and CAL were performed on each patient group.</div></div><div><h3>Conclusion</h3><div>Compared to the placebo and standard group, the test group was remarkably associated with improved GI, PI, PPD, and CAL at the end of the study. Therefore, drug-loaded nanofiber was found to be efficacious in treating periodontal diseases and may be appropriate as an alternative treatment.</div></div><div><h3>Clinical significance</h3><div>The fabricated PCL-GE-A2 nanofiber mat was more cost-efficient, minimized the dosage amount, and dosage frequency, and showed no adverse effects or discomforts with increased patient compliance.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1336-1346"},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of root and root canal morphology in maxillary molars with fused roots using Cone Beam Computer Tomography (CBCT) in a Sri Lankan population","authors":"Ruvienath Daham Weerasinghe Rajapaksa ,&nbsp;Manil Christopher Nishan Fonseka ,&nbsp;Ruwan Duminda Jayasinghe ,&nbsp;Rasika Manori Jayasinghe","doi":"10.1016/j.jobcr.2025.08.016","DOIUrl":"10.1016/j.jobcr.2025.08.016","url":null,"abstract":"<div><h3>Introduction</h3><div>Root fusion is considered to present when there is no evidence of periodontal space or presence of bone between the different roots of the molar at any apical level to the bifurcation area. Fused roots in maxillary molars pose important clinical implications, mainly in the field of endodontics. Based on the wide variations in previous studies done in different populations and the clinical implications, the present study is aimed to assess root and root canal morphology in maxillary first and second molars with fused roots in a Sri Lankan population.</div></div><div><h3>Material and methods</h3><div>A descriptive study was conducted by evaluating all CBCT scans stored at Division of Oral Medicine and Radiology, Faculty of Dental sciences, University of Peradeniya which were taken from January 1st, 2017 to December 31st, 2019. To characterize the type of root fusion of maxillary molars, classification of Zhang et al., in 2014 was used.</div></div><div><h3>Results</h3><div>Out of one thousand twenty upper first molars (1020), fifty two had fused roots (5.098 %) and out of one thousand ninety-six upper second molars (1096), 473 (43.15 %) had fused roots. The commonest pattern of fusion noted in first molars was type 1 (42.3 %) and in second molars was type 2 (36.9 %).</div></div><div><h3>Conclusion</h3><div>The root and canal configurations of maxillary first and second molars in this population were consistent with previously reported data. Fused roots may present a complicated root canal system. These data may facilitate successful endodontic treatment. More studies in larger populations would provide more details in our population.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1297-1304"},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the need for general anesthesia versus inhalational sedation using modified mohan scale in pediatric dental patients","authors":"Sriram Swetha ,&nbsp;Bagmar R. Mehul ,&nbsp;Jayakumar Priya","doi":"10.1016/j.jobcr.2025.08.015","DOIUrl":"10.1016/j.jobcr.2025.08.015","url":null,"abstract":"<div><h3>Background</h3><div>Effective behavior management is vital in pediatric dentistry to ensure positive treatment in uncooperative children. General anesthesia (GA) and Inhalational sedation (IS) are some commonly used techniques, but an objective assessment tool is required to determine the most appropriate approach.</div></div><div><h3>Objective</h3><div>This study aims to assess the effectiveness of the Modified Mohan Scale in guiding the selection between GA and IS for pediatric dental patients.</div></div><div><h3>Methods</h3><div>Thirty children aged 3–6 years were evaluated using the Modified Mohan Scale, which considers American Society of Anesthesiologists (ASA) classification, treatment complexity, number of teeth affected, behavior during radiographic imaging, parental expectations, socioeconomic status, and Frankl's Behavior Rating Scale. Mann–Whitney <em>U</em> test was conducted after assessing normality with the Shapiro–Wilk test to compare the GA and IS groups.</div></div><div><h3>Results</h3><div>Significant differences were found in the total Modified Mohan Scale scores between the GA and IS groups (p = 0.0001). Additionally, subscales related to the number of teeth requiring treatment (p = 0.011) and behavior during radiographic imaging (p = 0.029) showed prominent variations, with higher scores indicating a greater need for GA.</div></div><div><h3>Conclusion</h3><div>The Modified Mohan Scale shows promising potential as a structured and consistent method for evaluating the need for GA versus IS in pediatric dental patients. The scale's application may support clinical decision-making and treatment planning. Future research with a larger sample size is recommended to validate its application for broader use.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1311-1315"},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediating effect of resilience on untreated dental caries among adults in Hyderabad, India: A cross-sectional study","authors":"Jagadeeswara Rao Sukhabhogi,&nbsp;Dolar Doshi,&nbsp;Pothulapally Priyanka,&nbsp;Ellora Reddy Thimmanagari","doi":"10.1016/j.jobcr.2025.08.017","DOIUrl":"10.1016/j.jobcr.2025.08.017","url":null,"abstract":"<div><h3>Background</h3><div>Resilience, a psychological trait associated with adaptability and stress management, has been increasingly recognized as a determinant of health outcomes. However, its role in oral health, particularly concerning untreated dental caries, remains under-explored. This cross-sectional study examines the mediating effect of resilience on untreated dental caries among adults visiting a Government Dental Hospital in Hyderabad.</div></div><div><h3>Materials and method</h3><div>A cross-sectional study was conducted among adult patients attending a government dental hospital. Resilience was assessed using the Adult Resilience Measure – Revised (ARM-R), while dental caries was evaluated using the WHO Oral Health Assessment Form. Structural Equation Modeling (SEM) was employed to analyze the mediating effects of sociodemographic factors and oral health behaviors in the relationship between resilience and untreated dental caries.</div></div><div><h3>Results</h3><div>A significant negative correlation was observed between resilience and untreated dental caries (p = 0.001), indicating that individuals with lower resilience levels had 12 times higher odds of developing dental caries. Employment status emerged as a key mediating factor (∗∗β = 0.30, p = ∗∗∗), with higher resilience levels being associated with better employment outcomes, which in turn influences access to dental care. Additionally, increased brushing frequency was identified as another mediator (β = 0.08, p = 0.02), suggesting that individuals with greater resilience are more likely to engage in positive oral health behaviors.</div></div><div><h3>Conclusion</h3><div>The findings highlight the protective role of resilience in oral health by influencing employment status and oral hygiene practices, thereby reducing the risk of untreated dental caries. These results underscore the need for integrating psycho-social interventions aimed at enhancing resilience in oral health promotion programs.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1305-1310"},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in tumor targeted polymeric nanoparticles for HNC treatment: Enhancing therapeutic efficacy via engineered and biocompatible drug delivery systems","authors":"Manoj Kumar Srinivasan ,&nbsp;Monisha Prasad","doi":"10.1016/j.jobcr.2025.08.012","DOIUrl":"10.1016/j.jobcr.2025.08.012","url":null,"abstract":"<div><div>Head and neck cancer (HNC) represents a heterogeneous group of malignancies with high global incidence and mortality rates. Traditional treatments for advanced HNC, such as combined radiotherapy and chemotherapy, often lead to debilitating long-term side effects that significantly affect patients quality of life. In response to these limitations, tumor-targeted polymer-based nanoparticles have emerged as a promising strategy to improve therapeutic efficacy while minimizing adverse effects. Polymeric nanoparticles comprised of natural or synthetic polymers and sized between 10 and 1000 nm have several benefits, including biocompatibility, adaptable drug release patterns, and the capacity to improve the solubility and stability of medicinal compounds. These nanoparticles can be engineered to target medications to tumor sites specifically, lowering systemic toxicity and enhancing treatment results. This review focuses on recent advances in the targeted therapy of HNC using polymeric nanoparticles such as chitosan, alginate, cyclodextrin, poly (lactic acid), poly (caprolactone), poly (ethylene glycol), dendrimers, and micelles. The emphasis is on their mechanisms, advantages, and potential to transform present HNC treatments.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1316-1330"},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of TGF-β1 with periodontitis patients in north Indian population","authors":"Ruchi Pandey ,&nbsp;Sumit Bhateja ,&nbsp;Lipika Gopal ,&nbsp;Ankita Chhabrani ,&nbsp;Anju Rana ,&nbsp;Puneet Batra","doi":"10.1016/j.jobcr.2025.08.021","DOIUrl":"10.1016/j.jobcr.2025.08.021","url":null,"abstract":"<div><h3>Introduction</h3><div>Periodontitis is a complex disease caused by environmental and genetic factors. The course of periodontal destruction is influenced by local, ecological, and, predominantly, by genetic factors. Therefore, early detection of the disease through genomics and its course of destruction can be altered through genetic engineering, which can be a major breakthrough in the eradication of periodontitis.</div></div><div><h3>Aim</h3><div>The purpose of this study is to find the genetic association of TGF-β1 gene polymorphisms in causing periodontitis in the North Indian population.</div></div><div><h3>Materials and methods</h3><div>A total of 364 subjects were selected in the present study, and they were divided into two groups, viz. Healthy (Group A) and Periodontitis (Group B), with 182 subjects in each. The detailed case history was recorded, buccal swab samples were collected and stored in the sterile medium at 4 °C. Correlation studies on gene polymorphism with the disease occurrence were carried out by estimating allele frequency, Hardy-Weinberg equilibrium (HWE), and chi-square test (GenAlex package), and linkage disequilibrium and haplotype analysis (SHEsis).</div></div><div><h3>Result</h3><div>The gene TGF-β1 showed a high rate of polymorphism in the north Indian population. The TGF-β1 gene showed polymorphism in the studied SNP sites. Among them, −29C/T and −788 C/T could differentiate the healthy and generalized periodontitis group; however, the localized periodontitis group could not be differentiated with any of the SNP loci.</div></div><div><h3>Conclusion</h3><div>The gene TGF-β1 showed association with periodontitis in the North Indian Population.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1331-1335"},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposing a revised Hanau's formula for determining lateral condylar guidance: A comparative study","authors":"Jayant N. Palaskar ,&nbsp;Amit D. Hindocha ,&nbsp;Prashant D. Jadhav ,&nbsp;Pooja P. Hasabnis","doi":"10.1016/j.jobcr.2025.08.014","DOIUrl":"10.1016/j.jobcr.2025.08.014","url":null,"abstract":"<div><h3>Background</h3><div>Inaccurate lateral condylar guidance (LCG) recordings lead to occlusal interferences, increasing patient discomfort and chair-side time. This study aimed to assess and compare the accuracy of LCG calculated using Hanau's formula and right and left lateral records. It also seeks to provide a new formula for calculating LCG.</div></div><div><h3>Materials and methods</h3><div>An in-vivo, observational, cross-sectional study was conducted with hundred and sixty readings from twenty patients, divided into four groups (forty readings per group): Group A (control)- 2 frontal cephalograms (1st-maximum intercuspation and 2nd-5 mm lateral excursion), Group B- lateral records, Group C- Hanau's formula, and Group D-the new formula. Horizontal condylar guidance (HCG) values from protrusive records were used in Hanau's formula to calculate LCG. LCG values were also obtained from lateral records, frontal cephalograms (taken with lateral jigs), and the new formula. These values were tabulated and statistically evaluated using One-way ANOVA for group comparisons, Tukey's post hoc test for pairwise comparisons, and Shapiro-Wilk test for data normality.</div></div><div><h3>Results</h3><div>The mean LCG values for Group B were 32.3 (right) and 30.8 (left), and for Group D were 31.15 (right) and 31.75 (left), comparable to Group A of 33.17 (right) and 33.69 (left). The mean LCG values for Groups C were 15.33 (right) and 15.41 (left) and were found to be statistically significant with Group A.</div></div><div><h3>Conclusion</h3><div>LCG values obtained using the new proposed formula are closer to control values, making it a viable alternative to interocclusal records and more accurate than Hanau's formula.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1291-1296"},"PeriodicalIF":0.0,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in vivo comparative study of pure Mg and Mg-Zn alloy plates implanted in the mandible of New Zealand rabbits","authors":"Altaib Abdel Razik Mohammed ,&nbsp;Wafaa Yahia Alghonemy ,&nbsp;Naglaa I. Sarhan ,&nbsp;Abdalla Abdal-hay ,&nbsp;Reda Gaber Saleh","doi":"10.1016/j.jobcr.2025.08.009","DOIUrl":"10.1016/j.jobcr.2025.08.009","url":null,"abstract":"<div><h3>Background</h3><div>Biodegradable magnesium-based implants present significant potential for maxillofacial applications, particularly in mandible fixation, due to their osteoconductive properties and elimination of secondary removal surgeries.</div></div><div><h3>Objective</h3><div>The present study aimed to assess the in vivo biocompatibility and degradation behavior of pure magnesium (Mg) and Mg-Zn-Ca alloy plates implanted in the mandibles of New Zealand rabbits, providing insights into their clinical viability.</div></div><div><h3>Materials and methods</h3><div>Twenty-four male New Zealand White rabbits were divided into two groups and received titanium, pure Mg, and Mg-Zn-Ca alloy plates passively implanted into the mandibular bone surface. Animals were evaluated histologically and radiographically at 4 and 8 weeks postoperatively.</div></div><div><h3>Results</h3><div>Histomorphologic analysis demonstrated that all implants showed clinical stability throughout the observation period. While no signs of inflammation were observed in the pure Ti and pure Mg groups, the Mg-Zn-Ca alloy group exhibited moderate to severe inflammatory cell infiltration at 4 weeks and localized bone resorption at 8 weeks, as confirmed histologically. Remarkably, new bone formation was observed surrounding the Mg implants, with no evidence of fibrous capsule development. Mg-Zn-Ca biodegradation had no adverse systemic effects as the histological evaluations using H&amp;E staining of major organs revealed no significant abnormalities.</div></div><div><h3>Conclusion</h3><div>Mg-based materials show promise for maxillofacial fixation applications due to their osteogenic potential and biodegradability. However, the observed inflammatory responses and soft tissue effects highlight the need for further optimization of alloy composition and degradation control before clinical translation.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1279-1290"},"PeriodicalIF":0.0,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring knowledge gaps: A mixed-method cross-sectional study on Indian dental students’ perspectives and ethical awareness on artificial intelligence in dentistry","authors":"A. Subha ,&nbsp;R. Poornima ,&nbsp;M. Varshini","doi":"10.1016/j.jobcr.2025.08.005","DOIUrl":"10.1016/j.jobcr.2025.08.005","url":null,"abstract":"<div><h3>Introduction</h3><div>In the realm of healthcare, Artificial Intelligence (AI) has emerged as a powerful tool with the potential to enhance diagnostics, treatment planning, and patient care. This comprehensive survey was conducted among dental students in India to better understand their knowledge, attitudes, and perceptions of the integration of AI in dentistry.</div></div><div><h3>Materials and method</h3><div>A structured questionnaire that consisted of both closed-ended and open-ended questions was designed to gather data on the students’ demographic information, knowledge, attitudes, and perceptions towards AI in dentistry. Additionally, their perspectives on ethical consideration related to AI use was also evaluated. The survey was carried out using Google Forms among 1256 dentistry students from 15 different states in India. Descriptive statistics, and inferential statistical analysis such as Chi-square test were performed to obtain the results.</div></div><div><h3>Results</h3><div>1256 dental students responded to the survey and there was a predominantly positive view towards AI in Dentistry. 94 % believed AI would lead to significant advances in dentistry, with 79 % finding it exciting. AI's diagnostic capabilities were high, with 89 % agreeing it can be used definitively and 85 % for prognostic purposes. Over 90 % supported AI for radiographic diagnosis of dental caries, periodontal disease, jaw pathologies, and soft tissue lesions. However, 59 % disagreed that AI could replace dentists. Knowledge of AI applications increased with the year of study, with postgraduate students showing the highest awareness. However, only 26 % of participants were aware of the ethical implications of using AI in dentistry, while 44 % were unaware and 30 % were unsure.</div></div><div><h3>Conclusion</h3><div>Most of the participants were aware of the potential benefits of integrating AI in dentistry. There is a need to focus on educating students about ethical concerns when teaching AI in dental education.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1274-1278"},"PeriodicalIF":0.0,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro evaluation of multicomponent scaffold with kappa carrageenan, tendon extracellular matrix and calcium-magnesium silicate for periodontal bone regeneration","authors":"Supraja G.,&nbsp;S. Balaji Ganesh,&nbsp;Raja Kumar,&nbsp;Gurumoorthy Kaarthikeyan","doi":"10.1016/j.jobcr.2025.08.011","DOIUrl":"10.1016/j.jobcr.2025.08.011","url":null,"abstract":"<div><h3>Introduction</h3><div>Chronic periodontitis is a complex combinatory disease that eventually upsets periodontal tissues, ultimately leading to tooth loss. In vertical bone loss defects, therapy includes surgical procedures, such as flap surgery with debridement associated with scaffolds, grafts, or membranes. The aim of the study is to fabricate a novel carrageenan, Calcium Magnesium Silicate (CaMgSiO), and ovine extracellular matrix (ECM)-based scaffold for periodontal bone regeneration.</div></div><div><h3>Materials and methods</h3><div>To a 2 % carrageenan solution, 1 mg/mL of CaMgSiO and ECM was added. This homogenous solution was immersed in 0.15 M CaCl₂ solution. The divalent cations (Ca^+ 2 ions) were diffused into the gel and crosslinked to form a scaffold. The cross-linked scaffold (kappa carrageenan-CaMgSiO-ECM) was washed twice, underwent lyophilization and stored at 4 °C for further use. Scaffold characterization was done by means of X-ray diffraction (XRD), Raman spectrum, and scanning electron microscope (SEM), and energy dispersive spectroscopy (EDS). Then hemocompatibility assay, swelling analysis, and differentiation test were done to assess the biocompatibility of the scaffold.</div></div><div><h3>Result</h3><div>Calcium was observed with a weight percentage of 16.7 %, silicate with 27.6 wt%, and magnesium with 2.8 wt% via EDS spectra. Kappa carrageenan, CaMgSiO + ECM scaffold has good agreement with red blood cells with a minimal lysis of 2 % at the concentration of 10 mg/mL, and the differentiation test showed maximum proliferation of cells.</div></div><div><h3>Conclusion</h3><div>The novel CaMgSiO ECM scaffold significantly enhances cell proliferation; it is highly hemocompatible and has enhanced mineralization properties. Hence, incorporation of bioactive bioceramics induces stability as well as enhances the regeneration properties of the tendon-derived ECM.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1260-1265"},"PeriodicalIF":0.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144829562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}