Journal of oral biology and craniofacial research最新文献

{"title":"Effects of systemic berberine administration on oral mucosal wound healing: Histopathological and biochemical evaluation in rats","authors":"Ümit Solmaz ,&nbsp;Bilal Ege ,&nbsp;Onur Ceylan ,&nbsp;Miray Ege ,&nbsp;Mahmut Koparal","doi":"10.1016/j.jobcr.2026.101457","DOIUrl":"10.1016/j.jobcr.2026.101457","url":null,"abstract":"<div><h3>Background</h3><div>Berberine is one of the main alkaloids obtained from plants. Numerous experimental and clinical studies have shown that berberine has anti-inflammatory, antioxidant, and immunomodulatory effects. The aim of this study was to investigate the histopathological and biochemical effects of systemic berberine administration on wound healing in the oral mucosa of rats.</div></div><div><h3>Materials &amp; methods</h3><div>The study was conducted on 42 adult male Wistar Albino rats. The rats were randomly divided into two main groups: control and experimental. In both the experimental and control groups, a mucoperiosteal excisional wound defect with a diameter of 5 mm was created behind the rugae folds in the palatal region. Rats in the experimental group were administered berberine at a rate of 100 mg/kg/day via oral gavage. Control rats received saline. Serum cytokines were analyzed by ELISA. Wound areas dissected from surrounding tissues were histopathologically examined for ulceration, inflammatory cells, necrosis, vascularization, fibroblasts, and edema.</div></div><div><h3>Results</h3><div>No significant differences between groups in serum cytokine levels. Histopathological examination of polymorphonuclear leukocytes, necrosis, vascularization, and fibroblast levels showed no statistically significant differences between groups on any of the study days. Ulceration was significantly lower in the experimental group on days 3 to 7. Mononuclear cells were significantly lower in the experimental group on day 3, and edema was significantly lower on day 7.</div></div><div><h3>Conclusion</h3><div>Our study suggests that berberine may have potential positive effects on the early stages of wound healing in the oral mucosa. Further studies are needed to clarify its clinical potential.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 3","pages":"Article 101457"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147797494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic HA–tooth graft composite promotes rapid alveolar bone healing via IL-6 suppression and OCN enhancement","authors":"Dwi Wahyu Indrawati ,&nbsp;Ernie Maduratna Setiawatie ,&nbsp;Retno Pudji Rahayu ,&nbsp;Shafira Kurnia Supandi ,&nbsp;Rizky Briliant Syah Manurung ,&nbsp;Mohammed Ahmed Aljunaid ,&nbsp;Astari Puteri","doi":"10.1016/j.jobcr.2026.101451","DOIUrl":"10.1016/j.jobcr.2026.101451","url":null,"abstract":"<div><h3>Background</h3><div>Periodontitis leads to continuous alveolar bone destruction driven by persistent inflammation, particularly elevated interleukin-6 (IL-6). Bone healing after tooth extraction under periodontitis conditions is often impaired, highlighting the need for regenerative biomaterials that can regulate inflammation and support osteogenesis. The hyaluronic acid–bovine tooth graft (HA + BTG) combination has been proposed as a promising BioGel-Graft candidate for accelerated bone regeneration.</div></div><div><h3>Aim and objectives</h3><div>This study aimed to evaluate IL-6 and osteocalcin (OCN) expression as biomarkers of alveolar bone healing in <em>Porphyromonas gingivalis</em>–induced Wistar rats following HA + BTG administration and to compare its regenerative effects with control and HA-only groups.</div></div><div><h3>Methodology</h3><div>Thirty-six male Wistar rats were allocated into three groups (control, HA, and HA + BTG). After periodontitis induction and tooth extraction, the treatment materials were applied to the extraction sockets. Tissue samples collected on days 14 and 21 were analyzed using immunohistochemistry for IL-6 and OCN, along with hematoxylin and eosin (H&amp;E) staining to assess woven bone formation.</div></div><div><h3>Results</h3><div>The HA + BTG group showed a significant reduction in IL-6 expression and a significant increase in OCN expression and woven bone area compared with the control (p &lt; 0.05). An inverse trend between IL-6 and OCN reflected the transition from inflammation to osteogenesis.</div></div><div><h3>Conclusion</h3><div>HA + BTG enhances alveolar bone regeneration by suppressing inflammation and stimulating osteoblastic activity, supporting its potential as an innovative gel-syringe biomaterial for regenerative alveolar bone therapy.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 3","pages":"Article 101451"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of dentin substitutes and light curing modes on intrapulpal temperature: An in vitro analysis","authors":"Kritheka Kumaresan,&nbsp;Mathan Rajan Rajendran,&nbsp;Preethi Varadan","doi":"10.1016/j.jobcr.2026.101458","DOIUrl":"10.1016/j.jobcr.2026.101458","url":null,"abstract":"<div><h3>Background</h3><div>Deep caries with extensive dentin loss reduces the strength of the tooth. To overcome this, various dentin substitutes have been widely used. Photopolymerization of such dentin substitutes can have an influence on the intrapulpal temperature of tooth which can lead to an increased risk of irreversible damage to the pulpal tissue. The mode of light curing can also have an influence on the rise in the intrapulpal temperature.</div></div><div><h3>Aim</h3><div>To evaluate the influence of Dentin Substitutes along with alternative Light Curing Modes on the rise in Intrapulpal Temperature.</div></div><div><h3>Materials and methods</h3><div>A single extracted human mandibular first premolar was used in the study. The root was resected 2 mm below the cementoenamel junction, and the occlusal surface was flattened to maintain 2 mm of remaining dentin thickness. A K- type thermocouple was introduced into the pulp chamber to record intrapulpal temperature during polymerization. Brilliant NG composite served as the control (Group 1), while SDR Plus (Group 2) and EverX Flow (Group 3) were the Dentin substitutes tested. Each group was further divided into three subgroups based on curing mode: Continuous (Subgroup A), Ramped (Subgroup B), and Pulsed (Subgroup C). Mean temperature rise was analyzed using one-way ANOVA and Tukey's post-hoc test.</div></div><div><h3>Results</h3><div>Group 3 revealed a significantly lower (p value &lt; 0.001) rise in intrapulpal temperature compared to Group 1 and Group 2. Among the different light curing modes, Subgroup C showed a significantly lower (p value = 0.033) rise in intrapulpal temperature compared to Subgroup A and Subgroup C.</div></div><div><h3>Conclusion</h3><div><ul><li><span>•</span><span><div>Ever X Flow resulted in a significantly lower rise in intrapulpal compared to the Control and SDR Plus group.</div></span></li><li><span>•</span><span><div>Pulsed mode of curing resulted in a significantly lower rise in intrapulpal temperature compared to the Continuous and Ramped curing modes.</div></span></li></ul></div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 3","pages":"Article 101458"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147797409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfer learning models in the detection of pulp calcifications- A preliminary study","authors":"Shishir Shetty ,&nbsp;Wael Talaat ,&nbsp;Sausan AlKawas ,&nbsp;Natheer Al-Rawi ,&nbsp;Mais Sadek ,&nbsp;Lubna Elayyan ,&nbsp;Mohammed Alsaegh ,&nbsp;Sangeetha Narasimhan ,&nbsp;Hiba Al- Daghestani ,&nbsp;Dilber Uzun Ozsahin ,&nbsp;Leena R. David","doi":"10.1016/j.jobcr.2026.101462","DOIUrl":"10.1016/j.jobcr.2026.101462","url":null,"abstract":"<div><h3>Background</h3><div>Pulp calcifications (PC) play a major role in the clinical outcome of endodontic treatment. Dentists use different radiographic modalities for detecting the presence of PCs. Transfer learning models have shown promising results with low computational resources in the detection and classification of diseases and conditions in dental radiographs. The aim of the present study was to evaluate the performance of transfer learning models in the detection of PCs in cropped panoramic radiographs (PRs).</div></div><div><h3>Materials and methods</h3><div>Two calibrated examiners collected 240 cropped PR images (120 with PCs and 120 without PCs) of maxillary and/or mandibular posterior teeth. The images were preprocessed using CLAHE (Contrast Limited Adaptive Histogram Equalization) and then augmented. Three pre-trained models, VGG16, ResNet101V2, and MobileNetV2, were used for classification of the images. A Fine-tuning approach was used for training the model.</div></div><div><h3>Results</h3><div>The inter-rater reliability among the five examiners was 0.91. VGG 16 was the best-performing model with training, validation, and test accuracies of 0.80, 0.85, and 0.85, respectively. VGG16 showed a precision of 0.84, a recall of 0.87, an F1-score of 0.86, and an AUC of 0.93. ResNet101V2 and MobileNetV2 showed test accuracies of 69% and 50%, respectively.</div></div><div><h3>Conclusions</h3><div>The Transfer-learning model VGG 16 outperformed other models in the detection of PCs in cropped PRs. Due to the use of cropped PRs, the model cannot be generalized; however, future work will be aimed at attaining similar performance metrics in uncropped PRs in larger datasets.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 3","pages":"Article 101462"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root-level vulnerability and lingual plate fracture risk in mandibular third molars: A retrospective CBCT-based cross-sectional study","authors":"Son Hoang Le ,&nbsp;Bich-Ly Thi Nguyen","doi":"10.1016/j.jobcr.2026.101461","DOIUrl":"10.1016/j.jobcr.2026.101461","url":null,"abstract":"<div><h3>Background</h3><div>Prior studies investigating the relationship between lingual plate (LP) fracture risk and mandibular third molar (M3M) anatomy have produced inconsistent findings. Factors such as age, sex, impaction depth, and buccolingual angulation have shown variable associations with fracture risk. Importantly, M3M rotational angulation has rarely been evaluated. While most research has focused on the middle and apical thirds of the root, one clinical study suggested the cemento-enamel junction may represent a structurally vulnerable site.</div></div><div><h3>Objectives</h3><div>This study aimed to identify anatomical and demographic risk factors associated with LP fracture across the coronal, middle, and apical thirds of the M3M root.</div></div><div><h3>Methods</h3><div>Cone beam computed tomography (CBCT) images of 300 mandibular third molars were retrospectively reviewed. Data collected included patient sex, age, tooth side, and spatial alignment. M3M root length was measured and divided into coronal, middle, and apical thirds. The thinnest LP region at each level was recorded, and fracture risk was classified into three categories: low, moderate, and high. Ordinal logistic regression was used to determine significant risk factors.</div></div><div><h3>Results</h3><div>Fracture risk was significantly lower in the coronal third compared to the middle and apical thirds. Younger patients exhibited higher fracture risk at all levels. Greater root length increased risk at the apical third. Horizontal impaction level II, mesial and horizontal angulations, and buccal inclination were associated with elevated risk, while lingual inclination and rotational angulation reduced risk.</div></div><div><h3>Conclusion</h3><div>Age, root morphology, impaction depth, and three-dimensional M3M angulations are key predictors of LP fracture risk at distinct root levels.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 3","pages":"Article 101461"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147797410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of cetylpyridinium chloride–modified EDTA, sequential, and continuous chelation on smear layer removal and dentin erosion","authors":"Huda Hassan Khairy,&nbsp;Alaa Jawad Kadhim","doi":"10.1016/j.jobcr.2026.101459","DOIUrl":"10.1016/j.jobcr.2026.101459","url":null,"abstract":"<div><h3>Objective(s)</h3><div>Evaluate the effectiveness of cetylpyridinium chloride (cpc) addition to EDTA (CPC-modified EDTA) compared to standard sequential (SC) and continuous (CC) chelation protocols in removing the smear layer and dentine erosion.</div></div><div><h3>Materials and methods</h3><div>The concentration of CPC was determined by pilot research based on surface tension and contact angle (0.2%). In the current study, sixty single roots-human mandibular premolars were divided into five groups (n = 12) according to irrigation protocol:sequential chelation using CPC-modified EDTA; conventional Sequential chelation using EDTA; continuous chelation using NaOCl mixed with HEDP; NaOCl alone and normal saline alone. The roots were separated longitudinally to evaluate the smear layer and dentine erosion utilizing SEM; and the obtained data were analyzed using Kruskal-Wallis and Mann-Whitney U tests.</div></div><div><h3>Results</h3><div>Similar effectiveness was observed between the sequential and continuous chelation protocols when EDTA alone was used (P &gt; 0.05), However, CPC-modified EDTA used in the sequential protocol showed significantly greater smear layer removal than continuous chelation protocol particularly in the apical third(P &lt; 0.05), while a similar effect on dentin erosion was observed among groups (P &gt; 0.05).</div></div><div><h3>Conclusion(s)</h3><div>The addition of a surfactant to the root canal irrigant showed better results; however, the difference was statistically significant only in the apical third At the apical third, CPC-modified EDTA demonstrated significantly better smear layer removal compared to the CC protocol, while showing a similar effect on dentin erosion compared to both SC and CC protocols.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 3","pages":"Article 101459"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147797493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of ozone therapy and antimicrobial photodynamic therapy in periodontitis: A randomized clinical trial","authors":"Shilpi Mangal ,&nbsp;Shalini Kaushal ,&nbsp;Nand Lal ,&nbsp;Anjani Kumar Pathak ,&nbsp;Bhaskar Agarwal ,&nbsp;Suruchi Shukla","doi":"10.1016/j.jobcr.2025.12.011","DOIUrl":"10.1016/j.jobcr.2025.12.011","url":null,"abstract":"<div><h3>Background</h3><div>This randomized clinical trial evaluated the comparative efficacy of ozone therapy and ICG-mediated antimicrobial photodynamic therapy as adjunctive to scaling and root planing in the management of periodontitis, by assessing clinical outcomes, inflammatory biomarkers, specifically interleukin-1β (IL-1β) and Pentraxin-3 (PTX-3), and microbial load via quantitative PCR.</div></div><div><h3>Methods</h3><div>This is a parallel-arm, single-blinded, randomized controlled trial that included 36 patients with Stage III Grade B periodontitis, allocated randomly to three groups. Group 1 {Scaling and Root planing (SRP)}, Group 2 {SRP + Ozone Therapy (OT}), and Group 3 {SRP + Antimicrobial Photodynamic Therapy (aPDT)}. Clinical parameters, biochemical markers, and microbial count were recorded at baseline, 1 month, and 3 months.</div></div><div><h3>Result</h3><div>The change in Plaque Index (PI), Gingival Index (GI), Probing Pocket Depth (PPD), and Clinical Attachment Level (CAL) from baseline to 1 month and from baseline to 3 months was significantly higher in the SRP + OT group (<em>p</em> &lt; 0.001). Reduction in the microbial count of <em>Porphyromonas gingivalis</em> (Keystone pathogen) (Mean difference = −0.70, <em>p</em> &lt; 0.001) and <em>Tannerella forsythia</em> (−0.51, <em>p</em> &lt; 0.001), whereas <strong>SRP + aPDT provided superior suppression</strong> for <em>Fusobacterium nucleatum</em> (−0.76, <em>p</em> &lt; 0.001) and <em>Prevotella intermedia</em> (0.40, <em>p</em> &lt; 0.001). No meaningful difference was observed between the two adjuncts for <em>Treponema denticola</em> (<em>p</em> = 0.994).</div></div><div><h3>Conclusion</h3><div>All treatment modalities resulted in significant improvements in all the parameters over time. However, SRP + Ozone Therapy consistently achieved the improvement across all parameters, when compared to SRP alone or SRP with aPDT.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101393"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145957725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of canine-guided and bilateral balanced occlusion appliances in managing TMJ disc displacement with Reduction-A randomised clinical trial","authors":"Renita Lorina Castelino ,&nbsp;Chethan Hegde ,&nbsp;Srikant Natarajan","doi":"10.1016/j.jobcr.2026.101412","DOIUrl":"10.1016/j.jobcr.2026.101412","url":null,"abstract":"<div><h3>Background</h3><div>Among the intracapsular disorders of the temporomandibular joint (TMJ), disc displacement with reduction (DDwR) occurs most frequently. Canine-guided orthotic appliances may increase loading on the contralateral TMJ during eccentric movements due to the leverage effect generated on the working side whereas bilateral balanced occlusion appliances may promote uniform force distribution, reducing contralateral joint stress in patients with DDwR. Evidence comparing the effectiveness of canine-guided and bilateral balanced occlusion orthotic appliances in the management of DDwR remains limited.</div></div><div><h3>Aim</h3><div>This study compared the effectiveness of canine-guided and bilateral balanced occlusion orthotic appliances in reducing pain, assessed using the Visual Analog Scale (VAS), in patients with DDwR.</div></div><div><h3>Material and methods</h3><div>This study included 68 patients with painful TMJ DDwR (RDC/TMD Group IIa). Participants were randomly assigned to two groups: Group 1 received a canine-guided orthotic appliance, and Group 2 received a bilateral balanced occlusion orthotic appliance. Pain intensity was assessed using the VAS at baseline, 15, 30, and 90 days.</div></div><div><h3>Results</h3><div>Mann-Whitney <em>U</em> test was used for group comparisons which demonstrated Group 2 achieved significantly lower VAS scores during early assessments, while Group 1 exhibited more notable reductions between 30 and 90 days. Wilcoxon test was used to assess improvement within groups which demonstrated significant improvement in both the groups with p value &lt; 0.001.</div></div><div><h3>Conclusions</h3><div>The bilateral balanced occlusion appliance produced faster pain reduction with lower VAS scores, particularly between 15 and 30 days. The canine-guided appliance showed greater pain reduction between 30 and 90 days. Both interventions effectively reduced pain.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101412"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of type I collagen on TLR-3 induced MMP-13 expression in human periodontal ligament fibroblasts","authors":"Ratthawut Namwad ,&nbsp;Pitchaya Chaiyaraksa ,&nbsp;Nirada Dhanesuan ,&nbsp;Siriluck Tiranathanagul","doi":"10.1016/j.jobcr.2026.101420","DOIUrl":"10.1016/j.jobcr.2026.101420","url":null,"abstract":"<div><h3>Background</h3><div>This study examined the effects of type I collagen, alone and in combination with poly I:C—a toll-like receptor 3 (TLR3) agonist—on matrix metalloproteinase-13 (MMP-13) expression and wound healing in human periodontal ligament (hPDL) fibroblasts.</div></div><div><h3>Methods</h3><div>hPDL fibroblasts were cultured and divided into four treatment groups: control, type I collagen (50 μg/mL), poly I:C (10 μg/mL), and a combination of type I collagen with poly I:C. Cytotoxicity was evaluated using the MTT assay. Cell migration was assessed via scratch assay at 0, 24, and 48 h. MMP-13 expression was quantified at both the mRNA and protein levels by real-time PCR and ELISA, respectively.</div></div><div><h3>Results</h3><div>After 24 h, the MTT assay indicated that none of the four treatment groups exhibited cytotoxicity toward hPDL fibroblasts. In the scratch assay at 24 and 48 h, type I collagen group demonstrated the fastest wound closure, whereas the poly I:C group showed the slowest migration. Regarding MMP-13 expression, the combination group displayed the highest mRNA levels, while ELISA revealed that both the combination and poly I:C groups had the greatest protein expression relative to the control.</div></div><div><h3>Conclusion</h3><div>This study provides evidence for an interaction between extracellular matrix signals and innate immune activation. Type I collagen promoted hPDL fibroblast migration, whereas poly I:C—a TLR3 agonist—upregulated MMP-13 expression, with the greatest effect observed in combination with collagen.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101420"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical profile and prevalence of odontogenic keratocysts in children and adolescents: A systematic review","authors":"Jéssica da Silva Cunha ,&nbsp;Raisa Severino-Lazo ,&nbsp;Joaquim Felipe Junior ,&nbsp;Allan Vinícius Martins-de-Barros ,&nbsp;Julliana Carvalho ,&nbsp;Belmiro Cavalcanti do Egito Vasconcelos ,&nbsp;Marianne Vasconcelos Carvalho","doi":"10.1016/j.jobcr.2026.101414","DOIUrl":"10.1016/j.jobcr.2026.101414","url":null,"abstract":"<div><div>Odontogenic keratocyst (OKC) exhibits aggressive behavior and a high potential for recurrence. Despite being well-documented in adults, its characteristics in the pediatric population remain poorly understood. This systematic review aimed to analyze the clinical profile and treatment modalities of OKCs in children and adolescents, as well as their association with recurrence and follow-up duration. This systematic review was conducted following PRISMA guidelines and registered in PROSPERO (CRD42024612291). Risk of bias was assessed using the ROBINS-E tool, and statistical analysis was performed with the chi-square test. A total of 34 studies published between 2014 and 2024 were included, comprising 98 pediatric cases, both sporadic and syndromic. Both groups showed a slight male predominance, with a mean age of 12 years, and the most common symptom was extraoral swelling, with or without pain. The cases showed a predominance of lesions in the posterior mandible. Conservative approaches were the most frequently adopted treatment modality. The chi-square test revealed a statistically significant association between treatment modality and recurrence (p = 0.031), as well as between recurrence and follow-up duration (p = 0.005). Despite the variety of therapeutic strategies, recurrence was more strongly associated with follow-up duration than with the treatment approach itself. Based on these findings, we strongly recommend that all pediatric patients diagnosed with OKC undergo clinical and radiographic monitoring for a minimum of five years, regardless of the treatment performed, to ensure early detection and management of late recurrences.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101414"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}