Journal of oral biology and craniofacial research最新文献

{"title":"Molecular effects of areca nut–derived compounds on human oral cancer cells: a systematic review of in vitro evidence","authors":"Visilmi Kaffah Putri Ayna,&nbsp;Irene Anastasia Tampubolon,&nbsp;Kharinna Widowati,&nbsp;Andari Sarasati,&nbsp;Nurina Febriyanti Ayuningtyas,&nbsp;Meircurius Dwi Condro Surboyo","doi":"10.1016/j.jobcr.2026.101502","DOIUrl":"10.1016/j.jobcr.2026.101502","url":null,"abstract":"<div><h3>Objective</h3><div>Areca nut chewing is a major risk factor for oral potentially malignant disorders (OPMD) and oral squamous cell carcinoma (OSCC), yet the molecular mechanisms underlying its carcinogenic effects remain incompletely integrated. This systematic review aimed to synthesize in vitro evidence on the molecular effects of areca nut extract and areca nut–derived alkaloids in human oral cancer cells.</div></div><div><h3>Methods</h3><div>This systematic review followed PRISMA 2020. PubMed, ScienceDirect, and Scopus were searched from inception to the final search date. Eligible studies used human oral squamous cell carcinoma cell lines or primary oral cancer cells exposed to areca nut extract or related compounds. Outcomes involving inflammation, proliferation, oxidative stress, oncogenic signaling, and tumor-suppressor regulation were qualitatively synthesized because of methodological heterogeneity.</div></div><div><h3>Results</h3><div>Twenty studies met the inclusion criteria. Areca nut–derived compounds were associated with increased pro-inflammatory mediators, including IL-1β, IL-6, IL-8, COX-2, and PGE2. They also altered cell proliferation and cell-cycle control, with increased proliferative markers and suppression of key checkpoint regulators. Oxidative stress was characterized by elevated intracellular and mitochondrial reactive oxygen species, oxidative DNA damage, lipid peroxidation, and impaired antioxidant defenses. Oncogenic and stress-responsive pathways, including NOTCH1, Akt, and Nrf2/HO-1, were activated, whereas p53, p27, SIRT1, and AP-1 transcription factors were downregulated. Findings for p21 were contradictory across studies.</div></div><div><h3>Conclusions</h3><div>In vitro evidence indicates that areca nut constituents induce coordinated inflammatory, proliferative, oxidative, and signaling alterations that support their carcinogenic role in human oral cancer cells.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101502"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148776787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and preliminary evaluation of tongue cleaner-based pH and glucose biosensors for salivary assessment","authors":"Zoya Hussain,&nbsp;Shakila Mahesh","doi":"10.1016/j.jobcr.2026.101506","DOIUrl":"10.1016/j.jobcr.2026.101506","url":null,"abstract":"<div><h3>Introduction</h3><div>Saliva reflects both oral and systemic health. Monitoring salivary pH and glucose using tongue cleaner-based biosensors may provide a simple non-invasive method for routine salivary assessment.</div></div><div><h3>Objective</h3><div>To develop and preliminarily evaluate separate tongue cleaner-based biosensors for salivary pH and glucose detection in healthy individuals and those with oral disease.</div></div><div><h3>Materials and methods</h3><div>Separate pH and glucose biosensors were fabricated on tongue cleaner platforms. The pH biosensor used an anthocyanin–chitosan–pullulan membrane, while the glucose biosensor incorporated glucose oxidase-functionalized carbon graphite and silver/silver chloride electrodes. Eighty participants aged 18–65 years were included: 40 with dental caries or periodontitis and 40 healthy controls. The glucose sensor was calibrated using glucose standards and validated against the GOD–POD assay.</div></div><div><h3>Results</h3><div>Salivary pH was significantly lower in the study group than controls (6.71 ± 0.41 vs 7.44 ± 0.22; p &lt; 0.001). Glucose resistance values were significantly higher in the study group (39.03 ± 15.54 kΩ vs 10.24 ± 1.13 kΩ; p &lt; 0.001), indicating elevated salivary glucose levels. Very large effect sizes were observed for pH, glucose resistance, and glucose levels. Potentiometric measurements strongly correlated with the GOD–POD assay (r = 0.96; p &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>The developed tongue cleaner-based biosensors demonstrated measurable differences in salivary pH and glucose between healthy individuals and those with oral disease. These low-cost, non-invasive biosensors show potential for salivary assessment, although further large-scale validation is required.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101506"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148776870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of temporomandibular joint morphological alterations after bilateral sagittal split ramus osteotomy - A systematic review and meta-analysis","authors":"Rasha Azees,&nbsp;K. Sarika,&nbsp;Anju James,&nbsp;Parvathy Ghosh,&nbsp;N.K. Sapna Varma,&nbsp;V.V. Ajith","doi":"10.1016/j.jobcr.2026.101483","DOIUrl":"10.1016/j.jobcr.2026.101483","url":null,"abstract":"<div><h3>Background</h3><div>Orthognathic surgery corrects skeletal deformities and restores facial and functional balance. This systematic review evaluated the effects of bilateral sagittal split ramus osteotomy (BSSRO) on temporomandibular joint (TMJ) morphology, including joint spaces, condylar height, axial angle, disc position, and muscle changes.</div></div><div><h3>Methods</h3><div>Following PRISMA guidelines, a comprehensive search of PubMed, Scopus, Cochrane Library, and Google Scholar was conducted. Two authors independently performed critical appraisal and data extraction. Standardized mean differences with 95% confidence intervals were used to evaluate surgical effects on TMJ morphology.</div></div><div><h3>Results</h3><div>A total of 26 studies were included, with ten contributing to meta-analysis. Most studies showed no significant postoperative changes in joint spaces in either malocclusion group. Two long-term studies showed a significant reduction in posterior joint space in Class II patients [SMD: 0.97 (95% CI 0.61, 1.33)], with no change in Class III patients. In Class III cases, two studies showed a significant long-term decrease in condylar height [SMD: 0.37 (95% CI 0.08, 0.66)], and three studies demonstrated a reduction in axial condylar axis angle [SMD: 0.77 (95% CI 0.44, 1.10)]. Non-pooled studies reported largely unchanged disc positions, occasional improvement in Class III cases, and inconsistent postoperative changes in masseter and pterygoid muscle morphology.</div></div><div><h3>Conclusions</h3><div>BSSRO produces malocclusion-specific TMJ adaptations, with posterior joint space reduction in Class II, condylar remodeling in Class III, and stable disc position in both groups.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101483"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13325305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Moringa oleifera on inflammation, oxidative stress, and bone-related changes in a rat model of experimental periodontitis","authors":"Mert Keles,&nbsp;Fatma Ucan Yarkac,&nbsp;Rahim Kocabas,&nbsp;Fahriye Kilinc","doi":"10.1016/j.jobcr.2026.101503","DOIUrl":"10.1016/j.jobcr.2026.101503","url":null,"abstract":"<div><h3>Background</h3><div>This study evaluated the effects of <em>Moringa oleifera</em> (MO) on inflammatory, oxidative stress, and bone-related changes in a rat model of ligature-induced experimental periodontitis.</div></div><div><h3>Methods</h3><div>Forty male Wistar rats were randomly assigned to five groups: healthy control (C), periodontitis (P), and periodontitis treated with MO at 100, 200, or 400 mg/kg/day (P + MO100, P + MO200, P + MO400). Periodontitis was induced by placing silk ligatures around the mandibular first molars for 14 days, followed by 4 weeks of daily oral MO administration. Serum levels of IL-1β, IL-6, TNF-α, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were measured by ELISA. Histopathological evaluation included inflammatory cell, osteoblast, and osteoclast counts, and histomorphometric analysis assessed alveolar bone level (ABL). Group comparisons were performed using the Kruskal–Wallis test with Dunn's post-hoc test and Holm adjustment.</div></div><div><h3>Results</h3><div>Significant overall group differences were observed for inflammatory cytokines, oxidative stress, antioxidant parameters, histopathological findings, and ABL. Compared with the P group, the P + MO200 and P + MO400 groups showed a more favorable profile, particularly for IL-6, IL-1β, SOD, GPx, and MDA after multiple-comparison adjustment. Histopathological findings also supported a more favorable pattern in these higher-dose groups. Although ABL differed significantly overall, pairwise differences were limited after adjustment. Serum urea, creatinine, ALT, and AST levels did not differ significantly among groups.</div></div><div><h3>Conclusion</h3><div>MO was associated with favorable anti-inflammatory, antioxidant, and bone-related changes in experimental periodontitis, with clearer effects at 200 and 400 mg/kg/day. Further studies are needed to confirm its translational potential.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101503"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13427545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adherence to national guidelines for responsible and ethical health communication through digital media across the orthodontic profession: From influence to accountability","authors":"Prachi Anil Bhorawat,&nbsp;Vaibhav Kumar,&nbsp;Ramola Ashish Gaikwad,&nbsp;Meghna Vandekar","doi":"10.1016/j.jobcr.2026.101498","DOIUrl":"10.1016/j.jobcr.2026.101498","url":null,"abstract":"<div><h3>Background</h3><div>Rapid expansion of digital media has transformed healthcare communication, and social media platforms are now widely used by the orthodontic profession for professional promotion, patient education, and dissemination of health information. While these platforms create valuable engagement opportunities, they raise concerns regarding professional accountability, ethical responsibility, and adherence to regulatory guidelines.</div></div><div><h3>Aim</h3><div>To assess awareness of and adherence to the National Guidelines for Responsible and Ethical Health/Wellness Communication through Digital Media in India among orthodontists and orthodontic residents, and to identify factors influencing compliance.</div></div><div><h3>Materials and methods</h3><div>A descriptive cross-sectional survey was conducted among practising orthodontists and orthodontic residents across India using the English version of the validated DISCOVER questionnaire, evaluating six domains: awareness, compliance, oversight, verification, ethics, and responsibility. The questionnaire was administered as a structured online form and responses were captured anonymously. Descriptive and inferential analyses were performed.</div></div><div><h3>Results</h3><div>A total of 1308 participants were surveyed. Awareness scores averaged 61.64 ± 28.37, indicating variability in understanding ethical and regulatory expectations, whereas Compliance was relatively high (84.74 ± 18.98). Oversight (70.45 ± 25.68) and Verification (81.52 ± 20.99) showed moderate engagement, while Ethics (96.25 ± 10.51) and Responsibility (96.46 ± 11.38) were highest. Orthodontists with more than 15 years of experience demonstrated higher Oversight, Verification, and Responsibility scores. Logistic regression identified Awareness, Oversight, and Verification as significant predictors of perfect compliance.</div></div><div><h3>Conclusion</h3><div>Although orthodontists and residents increasingly utilise digital platforms, adherence to national guidelines was inconsistent, with high ethical intent coexisting with limited regulatory awareness. Awareness, Oversight, and Verification emerged as the principal predictors of full compliance.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101498"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13393592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148578997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perceptions of essential oral health care and locus of control among adults in a developing country","authors":"K.S. Aparna,&nbsp;Ritu Duggal,&nbsp;Vijay P. Mathur,&nbsp;Harsh Priya,&nbsp;Bharathi M. Purohit,&nbsp;Sarah Paul,&nbsp;Neha Chauhan","doi":"10.1016/j.jobcr.2026.101487","DOIUrl":"10.1016/j.jobcr.2026.101487","url":null,"abstract":"<div><h3>Background</h3><div>Oral diseases are among the most prevalent global health problems, disproportionately affecting disadvantaged populations. Essential Oral Health Care (EOHC), endorsed by the World Health Organization, comprises basic promotive, preventive, and curative services that should be universally accessible. Public perceptions and psychological factors, particularly Locus of Control (LOC), strongly influence oral health behaviors and service use. This study assessed adults’ perceptions of Essential Oral Health Care (EOHC) services and examined the association of Locus of Control (LOC) with sociodemographic factors.</div></div><div><h3>Methods</h3><div>A cross-sectional study was conducted among 150 adults residing in Trilokpuri, New Delhi. Data were collected through interviewer-administered questionnaires on six domains: perceived needs, essential services, service adequacy, barriers to utilization, self-rated oral health and LOC. Statistical tests, including Mann- Whitney <em>U</em> test, Spearman correlation test, and multivariable logistic regression, were applied.</div></div><div><h3>Results</h3><div>Most participants (68.0%) recognized oral health as part of general health, yet only 34.0% reported a dental visit in the past year. Pain relief (42.0%) was the most commonly perceived essential service. Cost and waiting time were major barriers. Internal LOC was observed in 38.7% of participants and was positively associated with higher socioeconomic status (SES) (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Adults demonstrated limited awareness and narrow perceptions of EOHC, with substantial access and affordability barriers. Internal LOC was significantly associated with higher SES, underscoring the need for community-based oral health education and systemic strengthening of affordable EOHC services.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101487"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13356610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148430787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary metagenomic profiling of Neisseria, Dialister, and Filifactor species in periodontal health and disease using next-generation sequencing","authors":"Raghavi Rajasekar Ramani,&nbsp;Shivakumar Baskaran,&nbsp;Kurumathur Vasudevan Arun,&nbsp;Swarna Alamelu,&nbsp;Deepavalli Arumugamnainar","doi":"10.1016/j.jobcr.2026.101482","DOIUrl":"10.1016/j.jobcr.2026.101482","url":null,"abstract":"<div><h3>Background</h3><div>Periodontal diseases represent a complex dysbiosis-driven inflammatory condition, where the transition from health to gingivitis and periodontitis is accompanied by distinct microbial shifts. Emerging evidence highlights the significance of less-studied genera such as <em>Neisseria</em>, <em>Dialister</em>, and <em>Filifactor</em> in shaping periodontal outcomes. This study aimed to investigate the salivary distribution of <em>Neisseria</em>, <em>Dialister</em>, and <em>Filifactor</em> species across periodontal health, gingivitis, periodontitis, and gingival recession using next-generation sequencing (NGS).</div></div><div><h3>Methods</h3><div>Whole saliva samples were collected from 40 participants (10 per group) classified according to the American Academy of Periodontology criteria. Microbial DNA was extracted and subjected to 16S rRNA sequencing (V3–V4 region, Illumina MiSeq). Species-level classification was performed using the Human Oral Microbiome Database. Frequency distributions were compared across groups using Fisher's exact test, with significance set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>Distinct patterns were observed. Several commensal <em>Neisseria</em> species, including <em>N. subflava</em> (p = 0.001), <em>N. elongata</em>(p = 0.015), and <em>N. polysaccharea</em> (p = 0.001), showed significantly reduced prevalence in periodontitis compared with health and gingivitis. In contrast, <em>Dialister pneumosintes</em> exhibited a sharp increase in all diseased groups (p = 0.002). <em>Filifactor alocis</em> was markedly enriched in gingivitis, recession, and periodontitis (p = 0.011), suggesting its strong association with disease states.</div></div><div><h3>Conclusion</h3><div>The findings demonstrate a characteristic microbial shift in saliva: health-associated <em>Neisseria</em> species decline with disease progression, while anaerobic taxa such as <em>D. pneumosintes</em> and <em>F. alocis</em> expand. These results align with the polymicrobial synergy and dysbiosis model and underscore the potential of these species as salivary biomarkers for early detection and monitoring of periodontal disease.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101482"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13329486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic osteochondral regeneration of the TMJ condyle: integrating BMP-2 osteoinduction, MSC-mediated fibrochondrogenesis, and gradient 3D-printed scaffolds","authors":"Bernardo Correia Lima,&nbsp;Michelle Alonso Coutinho,&nbsp;Pedro Américo Felizardo dos Santos,&nbsp;Ricardo Grillo,&nbsp;Mariana Aparecida Brozoski,&nbsp;Fernando Melhem-Elias","doi":"10.1016/j.jobcr.2026.101501","DOIUrl":"10.1016/j.jobcr.2026.101501","url":null,"abstract":"<div><h3>Background</h3><div>Biological and biomaterial-based approaches for temporomandibular joint (TMJ) condylar reconstruction have advanced substantially, yet no unified regenerative strategy currently reproduces the native osteochondral unit, particularly the fibrocartilage–bone hierarchy and its biomechanical demands.</div></div><div><h3>Methods</h3><div>An integrative evidence synthesis was performed across four translational domains relevant to biomimetic condylar regeneration: (1) ultra-low-dose rhBMP-2 osteoinduction, (2) bilaminar osteochondral scaffolds, (3) MSC/TGF-β3–driven chondrogenesis, and (4) zonal or gradient 3D-printed constructs. Evidence was interpreted through mechanistic plausibility, scaffold design, safety constraints, and translational readiness for oral and maxillofacial surgery.</div></div><div><h3>Results</h3><div>Convergent findings support the feasibility of biomimetic osteochondral regeneration. Controlled BMP-2 delivery enables potent osteoinduction when spatially confined and dose-restricted. Bilaminar constructs reproducibly generate stratified cartilage–bone organization and improve interface integrity. MSC/TGF-β3 approaches promote fibrocartilage-like matrix formation, particularly when combined with instructive scaffolds. Gradient 3D printing enhances anatomical fidelity and transitional biomechanics, improving construct coherence across zones.</div></div><div><h3>Conclusions</h3><div>A multimodal regenerative framework integrating controlled osteoinduction, scaffold-based interface engineering, and biologically guided fibrocartilage regeneration may represent a promising translational pathway toward biologically engineered TMJ condylar reconstruction, although current evidence remains predominantly preclinical.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101501"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148776871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation and agreement between ICDAS and UniViSS caries detection systems among toddlers and preschoolers","authors":"K.C. Vignesh,&nbsp;Gnanasekaran Felsypremila,&nbsp;S. Ishvarya,&nbsp;P. Anitha,&nbsp;Haridoss Selvakumar,&nbsp;P.C. Arthi","doi":"10.1016/j.jobcr.2026.101499","DOIUrl":"10.1016/j.jobcr.2026.101499","url":null,"abstract":"<div><h3>Objectives</h3><div>To compare the agreement and concordance between ICDAS and UniViSS caries detection systems among toddlers and preschoolers.</div></div><div><h3>Methods</h3><div>This cross-sectional study included 281 children aged 2–6 years at a tertiary care hospital in South India. All primary molars were examined using both systems and converted to binary format (caries present vs. sound) for comparison, as full ordinal-scale comparison was not feasible given their non-equivalent scoring dimensions. Agreement was assessed using Cohen's Kappa coefficient; sensitivity, specificity, PPV, NPV, and likelihood ratios were calculated with ICDAS as the reference comparator. As ICDAS is a visual index rather than a histological gold standard, all parameters reflect inter-method concordance, not true diagnostic accuracy.</div></div><div><h3>Results</h3><div>The mean age was 5.01 years (SD = 1.07), with 52.7% females. Cohen's Kappa values ranged from 0.886 to 0.983, indicating almost perfect agreement. Against ICDAS as comparator, UniViSS showed inter-method sensitivity of 0.88–1.00, specificity of 0.96–0.99, PPV of 0.88–0.97, and NPV of 0.94–1.00; these values represent agreement with ICDAS and not true diagnostic accuracy. Overall concordance ranged from 95% to 99%.</div></div><div><h3>Conclusion</h3><div>UniViSS demonstrated almost perfect inter-method concordance with ICDAS for binary caries detection in primary molars of toddlers and preschoolers. UniViSS may serve as a practical clinical alternative to ICDAS for binary caries screening; however, findings should be interpreted in the context of the high-prevalence study population, and histological validation remains a priority.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101499"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148776858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of intralesional platelet-rich plasma and triamcinolone in management of oral submucous fibrosis: A prospective clinical study","authors":"Vipul Ughade,&nbsp;Sanyog Pathak,&nbsp;Sumit Asrani,&nbsp;Rajesh Dhirawani,&nbsp;Harshit Shrivastava","doi":"10.1016/j.jobcr.2026.101495","DOIUrl":"10.1016/j.jobcr.2026.101495","url":null,"abstract":"<div><h3>Background</h3><div>Oral Submucous Fibrosis (OSMF) is a chronic, progressive, potentially malignant disorder characterized by fibrosis of the oral mucosa, resulting in restricted mouth opening, burning sensation, mucosal blanching, and reduced cheek flexibility. Effective management remains challenging, and newer regenerative therapies such as platelet-rich plasma (PRP) have shown promising results.</div></div><div><h3>Aim</h3><div>To compare the efficacy of intralesional PRP and triamcinolone acetonide in improving mouth opening, burning sensation, cheek flexibility, and mucosal colour in patients with OSMF.</div></div><div><h3>Materials and methods</h3><div>This prospective comparative clinical study included 30 patients with OSMF (Group I–III according to the Ranganathan classification). Participants were randomly allocated into two groups of 15 each. Group I received intralesional PRP prepared using a standardized double-spin centrifugation technique, while Group II received intralesional triamcinolone acetonide. Six weekly injections were administered along with adjunct physiotherapy. Clinical parameters were assessed at baseline, 1 week, 6 weeks, and 3 months. Statistical analysis included two-way mixed ANOVA, Friedman test, Mann–Whitney <em>U</em> test, and Fisher's exact test.</div></div><div><h3>Results</h3><div>Both groups demonstrated significant improvement in all clinical parameters (p &lt; 0.001). No significant intergroup difference was observed in mouth opening. PRP showed significantly greater reduction in burning sensation at 3 months (p &lt; 0.001) and superior improvement in mucosal colour, whereas triamcinolone demonstrated greater improvement in cheek flexibility (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Both PRP and triamcinolone acetonide were effective in the management of OSMF. PRP provided superior symptomatic relief and mucosal improvement, whereas triamcinolone demonstrated greater improvement in cheek flexibility.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101495"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148582670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}