Journal of oral biology and craniofacial research最新文献

{"title":"Phytochemicals profile, cellular viabillity and platelet activity of Hibiscus rosa-sinensis Linn. leaf extract: An in vitro and ex vivo study","authors":"Liska Barus ,&nbsp;Coen Pramono Danudiningrat ,&nbsp;Andra Rizqiawan ,&nbsp;R. Aries Muharram ,&nbsp;I Gusti Putra Swabuana Purwoyudho ,&nbsp;Dewa Gede Raka Satria Wibawa ,&nbsp;Annisa Tri Maharani ,&nbsp;Namira Rizqi Hayyinah ,&nbsp;Alexander Patera Nugraha ,&nbsp;Ahmad K.M. Humidat ,&nbsp;Ni Putu Mira Sumarta","doi":"10.1016/j.jobcr.2025.07.008","DOIUrl":"10.1016/j.jobcr.2025.07.008","url":null,"abstract":"<div><h3>Objective</h3><div>to investigates the phytochemical content of <em>Hibiscus rosa-sinensis</em> Linn leaf extract (HrsL), its toxicity through effects on cell viability, and its impact on platelet activity.</div></div><div><h3>Methods</h3><div>The cytotoxicity of the leaf extract was evaluated using fibroblast cells, with various concentrations of the extract. The platelet activity of the HrsL, was assessed by treating blood and analysing the concentration of P-selectin and platelet aggregation with the agonists epinephrine, ADP, and collagen, compared to blood without extract treatment as a control.</div></div><div><h3>Results</h3><div>The <em>H. rosa-sinensis</em> leaf extract contains tannins, polyphenols, alkaloids, and saponins. HrsL was non-toxic at concentrations of 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, 62.5 mg/mL, 125 mg/mL, and 250 mg/mL after 24-h exposure, 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, 62.5 mg/mL, 125 mg/mL, and 250 mg/mL after 48-h exposure, and 15.6 mg/mL and 62.5 mg/mL after 72-h exposure. The effect on platelet activity at 250 mg/mL and 125 mg/mL concentrations of the extract showed a significant increase in P-selectin concentration. Platelet aggregation with the epinephrine agonist did not show significant differences at 7.8 mg/mL, while higher concentrations showed significantly lower aggregation. With the ADP and collagen agonists, no significant differences were observed at concentrations of 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, and 62 mg/mL, whereas concentrations above 62.5 mg/mL showed significantly lower platelet aggregation.</div></div><div><h3>Conclusion</h3><div>HrsL non-toxic at low concentrations and increases platelet P-selectin, It does not decrease platelet aggregation with the epinephrine agonist at 7.8 mg/mL, but it reduces aggregation at concentrations above 7.8 mg/mL. It does not decrease platelet aggregation with the ADP and collagen agonists at concentrations less than or equal to 62.5 mg/mL, but reduces aggregation at concentrations above 62.5 mg/mL.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1083-1090"},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transverse maxillary dimensions and upper airway morphology in mouth- and nasal-breathing children aged 10–12 years: A CBCT-based study","authors":"Rani Satiti ,&nbsp;Hendri Susanto ,&nbsp;Anrizandy Narwidina","doi":"10.1016/j.jobcr.2025.07.009","DOIUrl":"10.1016/j.jobcr.2025.07.009","url":null,"abstract":"<div><h3>Introduction</h3><div>Mouth breathing (MB) is a dysfunctional respiratory pattern that may affect craniofacial development by altering maxillary arch width and upper pharyngeal airway morphology. Early identification is critical to prevent long-term dentofacial and airway complications. This study aimed to compare maxillary arch width and upper airway morphology between mouth- and nasal-breathing children aged 10–12 years using Cone Beam Computed Tomography (CBCT).</div></div><div><h3>Materials and methods</h3><div>In this cross-sectional study, 30 children (15 mouth breathers and 15 nasal breathers) underwent CBCT imaging. Transverse maxillary arch dimensions were measured at four points: maxillary width at molars (MWM), intermolar width (IMW), maxillary width at canines (MWC), and intercanine width (ICW). Upper airway morphology was assessed using volumetric (nasopharyngeal volume [NPV], oropharyngeal volume [OPV]) and cross-sectional area (nasopharyngeal area [NPA], oropharyngeal area [OPA]) measurements. Independent <em>t</em>-tests were used to compare group differences with 95 % confidence level.</div></div><div><h3>Results</h3><div>The MB group showed significantly reduced maxillary arch widths (MWM, IMW, MWC, ICW) and diminished upper airway volume and area (NPV, OPV, NPA, OPA) compared to nasal breathers (<em>p</em> &lt; 0.001 for all parameters).</div></div><div><h3>Conclusion</h3><div>Mouth breathing in school-aged children is associated with measurable reductions in maxillary arch width and upper pharyngeal airway dimensions.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1064-1070"},"PeriodicalIF":0.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strength, weakness, opportunities and challenges (SWOC) experience of histopathology image analysis, enhanced by artificial intelligence","authors":"Narendra Nath Singh,&nbsp;Ankita Tandon,&nbsp;Pavithra Jayasankar","doi":"10.1016/j.jobcr.2025.07.013","DOIUrl":"10.1016/j.jobcr.2025.07.013","url":null,"abstract":"<div><div>Artificial intelligence (AI) is reshaping the landscape of oral cancer diagnosis through the analysis of digital imaging. By promoting early detection, enhancing diagnostic precision, and enabling personalised treatment approaches, AI holds the potential to significantly improve patient outcomes. However, it is important to carefully consider concerns related to bias, costs, data quality, and regulatory standards. Histopathology image analysis is critical for precise and early diagnosis, particularly cancer detection. It improves consistency, decreases subjectivity, and enables accurate assessment. Its combination with AI allows for faster diagnostics, remote consultations, sophisticated research, and personalised treatment methods, making it an essential tool in modern pathology and healthcare. To fully realise its promise in improving patient care and diagnostics for oral cancer, strategic investments, multidisciplinary cooperation, and strong regulatory frameworks are essential. This narrative review highlights the potential and challenges that lie ahead while advocating for a balanced approach that combines technical innovation with ethical and regulatory vigilance based on a comprehensive literature search and our team's personal experience.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1057-1063"},"PeriodicalIF":0.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating anterior and posterior alveolar trabecular patterns on periapical radiographs: Insights into bone mineral density in postmenopausal women","authors":"Rurie Ratna Shantiningsih ,&nbsp;Rini Widyaningrum ,&nbsp;Silviana Farrah Diba ,&nbsp;Adioro Soetojo ,&nbsp;Aga Satria Nurrachman ,&nbsp;Eha Renwi Astuti","doi":"10.1016/j.jobcr.2025.06.025","DOIUrl":"10.1016/j.jobcr.2025.06.025","url":null,"abstract":"<div><h3>Background</h3><div>The increasing aging population places postmenopausal women at higher risk of osteoporosis due to reduced bone mass. While Dual-Energy X-ray Absorptiometry (DXA) is the standard for measuring Bone Mineral Density (BMD), its cost and limited availability drive the need for alternative methods. Fractal Dimension (FD) analysis of trabecular bone patterns in periapical radiographs offers a cost-effective and accessible approach. This study examines FD differences in anterior and posterior mandibular regions as a potential early marker for osteoporosis in postmenopausal women.</div></div><div><h3>Materials &amp; methods</h3><div>A cross-sectional study analyzed periapical radiographs from 31 postmenopausal women with BMD and T-score data from DXA scans. FD analysis measured alveolar bone density (ABD) and D values in the anterior and posterior regions. One-way ANOVA tested regional differences, while Spearman's and Pearson's correlation assessed relationships between FD values, BMD, and T-score.</div></div><div><h3>Results</h3><div>No significant differences were found between anterior and posterior ABD and D values (p &gt; 0.05). ABD showed a significant positive correlation with BMD (r = 0.383, p &lt; 0.05), while D values did not. The T-score correlated significantly with anterior ABD (r = −0.357, p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Although no significant regional FD differences were found, ABD in periapical radiographs, particularly in the anterior region, may serve as a potential indicator for osteoporosis risk in postmenopausal women.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1077-1082"},"PeriodicalIF":0.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro evaluation of tear strength, antifungal effect, and polymicrobial resistance in Glycyrrhiza glabra–incorporated maxillofacial silicone","authors":"Harshini Sivakumar ,&nbsp;Jeyaraj Brintha Jei ,&nbsp;Balasubramaniam Muthukumar","doi":"10.1016/j.jobcr.2025.07.010","DOIUrl":"10.1016/j.jobcr.2025.07.010","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the tear strength, anti-candidal effect, and resistance to polymicrobial biofilm formation of maxillofacial silicone modified with varying concentrations of Glycyrrhiza glabra (0 %, 1.5 %, 2.5 %, and 5 %), focusing on enhancing its durability, antimicrobial properties, and overall performance.</div></div><div><h3>Methods</h3><div>A total of 192 samples were prepared and divided into groups based on Glycyrrhiza glabra concentrations, with further subgroups for each parameter with 48 samples in each concentration group. Tear strength was tested using a universal testing machine and expressed in N/mm. Anti-candidal and polymicrobial biofilm properties were assessed by inoculating samples with Candida albicans and a mixed culture of Candida albicans and <em>Staphylococcus aureus</em>, with colony counts measured at 24 and 48 h.</div></div><div><h3>Results</h3><div>Increasing the concentration of Glycyrrhiza glabra significantly enhanced tear strength and antimicrobial properties. Tear strength improved progressively, with Group D (5 % concentration) achieving the highest value of 12.43 N/mm. Anti-candidal efficacy showed a clear dose-dependent effect, with Group D reducing Candida albicans counts from 225.75 in Group A to 40.67 at 24 h and further to 25.17 at 48 h. Polymicrobial biofilm formation also decreased significantly, with Group D showing the lowest counts of 163.33 CFU at 24 h and 163.08 CFU at 48 h. Tukey's post hoc test revealed significant differences between treatment groups (<em>P</em> &lt; 0.001) and controls, highlighting that higher concentrations offered superior performance.</div></div><div><h3>Conclusion</h3><div>The incorporation of Glycyrrhiza glabra into maxillofacial silicone significantly enhances tear strength, anti-candidal, and anti-polymicrobial properties.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1071-1076"},"PeriodicalIF":0.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-functional azithromycin loaded polyvinyl alcohol and fucoidan hydrogel coating for titanium Implants: An experimental in vitro study","authors":"Maria Sharon Velraj,&nbsp;Arvina Rajasekar","doi":"10.1016/j.jobcr.2025.07.011","DOIUrl":"10.1016/j.jobcr.2025.07.011","url":null,"abstract":"<div><h3>Background</h3><div>Titanium-based implants are widely used in biomedical applications due to their superior mechanical properties and biocompatibility. However, the risk of postoperative infections and inadequate osseointegration remains a significant challenge. Hydrogels, with their tunable properties, offer a promising approach for enhancing the bioactivity of titanium surfaces. This study explored the development and characterization of an azithromycin-loaded PVA-fucoidan hydrogel coating on titanium and evaluated its physicochemical and biological properties.</div></div><div><h3>Aim</h3><div>To fabricate and characterize an azithromycin-loaded PVA-fucoidan hydrogel coated on titanium surfaces and assess their antibacterial, hemocompatibility, and biocompatibility properties.</div></div><div><h3>Materials and methods</h3><div>Commercially pure ASTM grade V titanium disks (n = 25) were prepared and treated via radio frequency argon plasma. PVA-fucoidan hydrogel was synthesized and loaded with azithromycin (1 % w/w), followed by electrophoretic deposition onto the titanium surface. Surface characterization was performed using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), and contact angle measurements. In-vitro drug release was analyzed using UV–Vis spectrophotometry. Antibacterial efficacy was tested against <em>Streptococcus mutans</em> and <em>Escherichia coli (n = 3</em> per <em>group)</em>. Hemocompatibility and cytotoxicity were evaluated via hemolysis assay, MTT assay, and live/dead cell assay (n = 3 replicates per assay).</div></div><div><h3>Results</h3><div>FTIR confirmed the successful integration of azithromycin without altering the hydrogel structure. SEM and AFM revealed uniform, textured coatings with enhanced roughness. Contact angle measurements indicated improved hydrophilicity (58°). Sustained azithromycin release was observed. The azithromycin-loaded coating exhibited superior antibacterial activity, larger inhibition zones, excellent hemocompatibility (0.8 % hemolysis), and 100 % cell viability in fibroblast cultures.</div></div><div><h3>Conclusion</h3><div>Azithromycin-loaded PVA-fucoidan hydrogel coating on titanium demonstrated promising physicochemical and biological properties, making it a potential candidate for enhancing the performance of titanium-based biomedical implants.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1043-1050"},"PeriodicalIF":0.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver nanoparticle-coated Bacteriophages: A novel therapeutic approach for combating Enterococcus faecalis in endodontic infections","authors":"Anabathula Praharsha ,&nbsp;Alpa Gupta ,&nbsp;Kanchan Bhardwaj ,&nbsp;Lubhansha Kumar ,&nbsp;Priya Khandelwal ,&nbsp;Saumya Verma","doi":"10.1016/j.jobcr.2025.06.024","DOIUrl":"10.1016/j.jobcr.2025.06.024","url":null,"abstract":"<div><h3>Introduction</h3><div>Endodontic infections, particularly those caused by <em>Enterococcus faecalis</em>, are a major cause of treatment failure due to their resistance to conventional antimicrobial treatments. This study explores the use of silver nanoparticles to coat bacteriophages targeting <em>E. faecalis</em>, aiming to improve their antimicrobial properties and biocompatibility for endodontic applications.</div></div><div><h3>Materials and methods</h3><div>Bacteriophages against Enteroccus faecalis were isolated from poultry samples using an agar overlay technique and purified through serial dilution and plaque assays. Silver nanoparticles were synthesized and coated onto the bacteriophages using a coacervation/precipitation method. The cytotoxicity of silver nanoparticles was assessed using an MTT assay on human gingival fibroblasts. Antimicrobial efficacy was evaluated through minimum inhibitory concentration (MIC) testing and zone of inhibition assays against <em>Streptococcus mutans</em> and <em>Porphyromonas gingivalis</em>. The structural characteristics of the silver nanoparticle-coated bacteriophages were analyzed using scanning electron microscopy (SEM).</div></div><div><h3>Results</h3><div>The bacteriophage isolation was confirmed by distinct plaque formation on <em>E. faecalis</em> cultures. The MTT assay revealed minimal cytotoxicity of 0.05 % silver nanoparticles, with cell viability greater than 91 % at all time points. MIC testing indicated that 0.05 % silver nanoparticles effectively inhibited bacterial growth, with significant inhibition zones (16.83 mm for <em>P. gingivalis</em> and 14.50 mm for <em>S. mutans</em>). SEM analysis showed successful coating of the bacteriophages with silver nanoparticles, with clear morphological changes and increased surface roughness.</div></div><div><h3>Conclusion</h3><div>Silver nanoparticle-coated bacteriophages represent a promising therapeutic approach to enhance bacteriophage stability and antimicrobial efficacy against <em>E. faecalis</em> in endodontic infections.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1051-1056"},"PeriodicalIF":0.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of inorganic ion configuration in enamel hypo mineralization","authors":"Gokul Vimal Thangaraj,&nbsp;Ramya Ramadoss,&nbsp;Sandhya Sundar,&nbsp;Suganya Panneer Selvam,&nbsp;Hema Shree K.,&nbsp;Nitya K.","doi":"10.1016/j.jobcr.2025.07.005","DOIUrl":"10.1016/j.jobcr.2025.07.005","url":null,"abstract":"<div><h3>Objectives</h3><div>Enamel hypomineralization is characterized by a reduction in the concentration of calcium (Ca) and phosphorus (P) in the enamel, along with an increase in carbon (C) concentration. This condition affects the mineral content of the enamel, leading to weakened and more susceptible teeth. These findings highlight the importance of understanding the inorganic ion configuration in enamel hypomineralization for effective restorative approaches and prevention strategies. It can pave the way for better therapeutic interventions. Thus, our study aims to investigate the characteristics of enamel hypoplasia in 5 extracted teeth using FE-SEM and EDX analysis.</div></div><div><h3>Methods</h3><div>The study investigated 5 human teeth with enamel hypoplasia and compared them with 1 normal control tooth. The teeth were cut into sections, dehydrated, and coated with platinum before being analyzed using a JSM-IT800 Field Emission Scanning Electron Microscope (FE-SEM) and Energy Dispersive X-ray Spectroscopy (EDX). After imaging, the specialized software was utilized to analyze and measure enamel hypoplasia's morphological traits and features of enamel hypoplasia. The SEM was used to examine the teeth's morphology, while the EDX was used to analyze the molecules.</div></div><div><h3>Findings</h3><div>Electron microscopic images exhibited altered topography on the surface of hypo-mineralized enamel. Elemental analysis showed the presence of Ca, P, Ag, Mg, C, K, and Cl, and their varied distribution. Normal enamel has 48.3 % oxygen and 27.5 % calcium. In hypoplastic enamel, oxygen increases to 49.1 % and calcium stays at 27.3 %. Phosphorus slightly decreases from 13.5 % to 13.3 %, and carbon decreases from 9.8 % to 8.7 %. There are no significant differences in sodium and chlorine. Enamel hypoplasia is linked to minor changes in elemental composition, with a significant decrease in carbon concentration. Standard deviations indicate the precision of the measurements.</div></div><div><h3>Novelty</h3><div>Prior research on enamel hypomineralization typically relies on comparative approaches, employing SEM and EDX for imaging and analysis. While calcium and phosphorus concentrations are frequently analyzed, the study's inclusion of additional elements and quantitative measurements offers a more comprehensive understanding. Advanced imaging techniques such as FE-SEM allow for detailed analysis of enamel structures. The findings contribute valuable insights to this diverse body of knowledge, crucial due to the complex nature of enamel hypomineralization. Thus, it could provide valuable insights for targeted remineralization techniques, ultimately preventing dental caries development.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1036-1042"},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced glass ionomer cement with bioactive additives for collagen synthesis and inflammation in pulp-rabbit teeth","authors":"Nattapon Rotpenpian ,&nbsp;Peerapon Sornying ,&nbsp;Sareepah Manmoo ,&nbsp;Krittee Dejyong","doi":"10.1016/j.jobcr.2025.07.007","DOIUrl":"10.1016/j.jobcr.2025.07.007","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to enhance conventional glass ionomer cement (GIC) by incorporating 15 % chitosan, 5 % bovine serum albumin (BSA), 0.05 % tricalcium phosphate (TCP), and 1 μg translationally controlled tumor protein (TCTP), resulting in an enhanced-GIC formulation. The study evaluated its adhesion properties, biocompatibility, and ability to promote pulp tissue healing in rabbit anterior teeth.</div></div><div><h3>Materials and methods</h3><div>The enhanced-GIC was tested in a rabbit model to assess its physical adhesion and biological effects on pulp tissue. Following cavity preparation and material placement, the teeth were observed for 21 days. Histological evaluations focused on inflammation, toxicity, and collagen synthesis in pulp tissue.</div></div><div><h3>Result</h3><div>The enhanced-GIC showed comparable adhesion properties to conventional GIC. Histological analysis revealed no significant inflammation or toxicity in the pulp tissue of either group. The enhanced-GIC group exhibited superior biocompatibility, demonstrated by increased lymphocyte infiltration and enhanced collagen synthesis within the pulp tissue, suggesting its potential for promoting tissue regeneration.</div></div><div><h3>Conclusion</h3><div>The enhanced-GIC formulation shows promise as a sublining material in restorative dentistry, offering benefits in pulp tissue healing and collagen formation, while maintaining adhesion comparable to conventional GIC. This study highlights enhanced-GIC's potential for use in dental restorative procedures, particularly for cases requiring pulp regeneration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1021-1028"},"PeriodicalIF":0.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological effects of two nano-composite resins on human gingival fibroblast (an in vitro study)","authors":"Mai Gamal Mahmoud ,&nbsp;Ali Shamaa ,&nbsp;Noura Mohammed Bakr ,&nbsp;Maha El Shahawy","doi":"10.1016/j.jobcr.2025.07.006","DOIUrl":"10.1016/j.jobcr.2025.07.006","url":null,"abstract":"<div><h3>Objective</h3><div>To elucidate the genotoxic and cytotoxic effects of two different nano-composite resins on primary human gingival fibroblasts (HGFs).</div></div><div><h3>Methods</h3><div>The HGFs were isolated, characterized, and classified into 3 groups. The control group consisted of untreated HGFs, the Omnichroma extract treated HGFs (the OMN group), and the 3M Filtek Z350 xt extract treated HGFs (ESPE group). The cell viability, mode of cell death and expression of <em>interleukin-1β</em> (<em>IL-1β</em>) and <em>interleukin-6</em> (<em>IL</em><strong><em>-</em></strong><em>6</em>) were investigated after 72 h and 24 h of resin extracts' pre-incubation with HGF.</div></div><div><h3>Results</h3><div>The isolated HGFs were characterized. The HGF viability was significantly higher in the OMN groups than ESPE groups at most of the concentrations. Total cell death was higher in the ESPE groups and at the OMN group at 72 h in comparison to the control and was higher in the ESPE groups compared to OMN groups. Furthermore, the <em>IL-1β</em> and <em>IL</em><strong><em>-</em></strong><em>6</em> levels in the OMN group at 72 h and in the ESPE groups were higher than the control one.</div></div><div><h3>Conclusion</h3><div>The cytotoxic and genotoxic effect of ESPE on HGF is more significant than the OMN.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1029-1035"},"PeriodicalIF":0.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}