Harshini Sivakumar , Jeyaraj Brintha Jei , Balasubramaniam Muthukumar
{"title":"甘草颌面硅胶抗撕裂强度、抗真菌效果及多微生物耐药性的体外评价","authors":"Harshini Sivakumar , Jeyaraj Brintha Jei , Balasubramaniam Muthukumar","doi":"10.1016/j.jobcr.2025.07.010","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the tear strength, anti-candidal effect, and resistance to polymicrobial biofilm formation of maxillofacial silicone modified with varying concentrations of Glycyrrhiza glabra (0 %, 1.5 %, 2.5 %, and 5 %), focusing on enhancing its durability, antimicrobial properties, and overall performance.</div></div><div><h3>Methods</h3><div>A total of 192 samples were prepared and divided into groups based on Glycyrrhiza glabra concentrations, with further subgroups for each parameter with 48 samples in each concentration group. Tear strength was tested using a universal testing machine and expressed in N/mm. Anti-candidal and polymicrobial biofilm properties were assessed by inoculating samples with Candida albicans and a mixed culture of Candida albicans and <em>Staphylococcus aureus</em>, with colony counts measured at 24 and 48 h.</div></div><div><h3>Results</h3><div>Increasing the concentration of Glycyrrhiza glabra significantly enhanced tear strength and antimicrobial properties. Tear strength improved progressively, with Group D (5 % concentration) achieving the highest value of 12.43 N/mm. Anti-candidal efficacy showed a clear dose-dependent effect, with Group D reducing Candida albicans counts from 225.75 in Group A to 40.67 at 24 h and further to 25.17 at 48 h. Polymicrobial biofilm formation also decreased significantly, with Group D showing the lowest counts of 163.33 CFU at 24 h and 163.08 CFU at 48 h. Tukey's post hoc test revealed significant differences between treatment groups (<em>P</em> < 0.001) and controls, highlighting that higher concentrations offered superior performance.</div></div><div><h3>Conclusion</h3><div>The incorporation of Glycyrrhiza glabra into maxillofacial silicone significantly enhances tear strength, anti-candidal, and anti-polymicrobial properties.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1071-1076"},"PeriodicalIF":0.0000,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In vitro evaluation of tear strength, antifungal effect, and polymicrobial resistance in Glycyrrhiza glabra–incorporated maxillofacial silicone\",\"authors\":\"Harshini Sivakumar , Jeyaraj Brintha Jei , Balasubramaniam Muthukumar\",\"doi\":\"10.1016/j.jobcr.2025.07.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><div>This study aimed to evaluate the tear strength, anti-candidal effect, and resistance to polymicrobial biofilm formation of maxillofacial silicone modified with varying concentrations of Glycyrrhiza glabra (0 %, 1.5 %, 2.5 %, and 5 %), focusing on enhancing its durability, antimicrobial properties, and overall performance.</div></div><div><h3>Methods</h3><div>A total of 192 samples were prepared and divided into groups based on Glycyrrhiza glabra concentrations, with further subgroups for each parameter with 48 samples in each concentration group. Tear strength was tested using a universal testing machine and expressed in N/mm. Anti-candidal and polymicrobial biofilm properties were assessed by inoculating samples with Candida albicans and a mixed culture of Candida albicans and <em>Staphylococcus aureus</em>, with colony counts measured at 24 and 48 h.</div></div><div><h3>Results</h3><div>Increasing the concentration of Glycyrrhiza glabra significantly enhanced tear strength and antimicrobial properties. Tear strength improved progressively, with Group D (5 % concentration) achieving the highest value of 12.43 N/mm. Anti-candidal efficacy showed a clear dose-dependent effect, with Group D reducing Candida albicans counts from 225.75 in Group A to 40.67 at 24 h and further to 25.17 at 48 h. Polymicrobial biofilm formation also decreased significantly, with Group D showing the lowest counts of 163.33 CFU at 24 h and 163.08 CFU at 48 h. Tukey's post hoc test revealed significant differences between treatment groups (<em>P</em> < 0.001) and controls, highlighting that higher concentrations offered superior performance.</div></div><div><h3>Conclusion</h3><div>The incorporation of Glycyrrhiza glabra into maxillofacial silicone significantly enhances tear strength, anti-candidal, and anti-polymicrobial properties.</div></div>\",\"PeriodicalId\":16609,\"journal\":{\"name\":\"Journal of oral biology and craniofacial research\",\"volume\":\"15 5\",\"pages\":\"Pages 1071-1076\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of oral biology and craniofacial research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S221242682500154X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221242682500154X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
In vitro evaluation of tear strength, antifungal effect, and polymicrobial resistance in Glycyrrhiza glabra–incorporated maxillofacial silicone
Objectives
This study aimed to evaluate the tear strength, anti-candidal effect, and resistance to polymicrobial biofilm formation of maxillofacial silicone modified with varying concentrations of Glycyrrhiza glabra (0 %, 1.5 %, 2.5 %, and 5 %), focusing on enhancing its durability, antimicrobial properties, and overall performance.
Methods
A total of 192 samples were prepared and divided into groups based on Glycyrrhiza glabra concentrations, with further subgroups for each parameter with 48 samples in each concentration group. Tear strength was tested using a universal testing machine and expressed in N/mm. Anti-candidal and polymicrobial biofilm properties were assessed by inoculating samples with Candida albicans and a mixed culture of Candida albicans and Staphylococcus aureus, with colony counts measured at 24 and 48 h.
Results
Increasing the concentration of Glycyrrhiza glabra significantly enhanced tear strength and antimicrobial properties. Tear strength improved progressively, with Group D (5 % concentration) achieving the highest value of 12.43 N/mm. Anti-candidal efficacy showed a clear dose-dependent effect, with Group D reducing Candida albicans counts from 225.75 in Group A to 40.67 at 24 h and further to 25.17 at 48 h. Polymicrobial biofilm formation also decreased significantly, with Group D showing the lowest counts of 163.33 CFU at 24 h and 163.08 CFU at 48 h. Tukey's post hoc test revealed significant differences between treatment groups (P < 0.001) and controls, highlighting that higher concentrations offered superior performance.
Conclusion
The incorporation of Glycyrrhiza glabra into maxillofacial silicone significantly enhances tear strength, anti-candidal, and anti-polymicrobial properties.
期刊介绍:
Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.