芙蓉植物化学成分、细胞活力和血小板活性的研究。叶提取物：体外和离体研究

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-07-23 DOI:10.1016/j.jobcr.2025.07.008

Liska Barus , Coen Pramono Danudiningrat , Andra Rizqiawan , R. Aries Muharram , I Gusti Putra Swabuana Purwoyudho , Dewa Gede Raka Satria Wibawa , Annisa Tri Maharani , Namira Rizqi Hayyinah , Alexander Patera Nugraha , Ahmad K.M. Humidat , Ni Putu Mira Sumarta

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引用次数: 0

摘要

目的研究芙蓉叶提取物（HrsL）的植物化学成分、对细胞活性的影响及对血小板活性的影响。方法采用成纤维细胞法对不同浓度提取物的细胞毒性进行评价。通过使用激动剂肾上腺素、ADP和胶原蛋白处理血液并分析p -选择素和血小板聚集的浓度来评估HrsL的血小板活性，并与不使用提取物处理的血液进行比较作为对照。结果桃红叶提取物中含有单宁、多酚、生物碱和皂苷。HrsL在暴露24 h后浓度为7.8 mg/mL、15.6 mg/mL、31.2 mg/mL、62.5 mg/mL、125 mg/mL和250 mg/mL，暴露48 h后浓度为7.8 mg/mL、15.6 mg/mL、31.2 mg/mL、62.5 mg/mL、125 mg/mL和250 mg/mL，暴露72 h后浓度为15.6 mg/mL和62.5 mg/mL均无毒。250 mg/mL和125 mg/mL提取物对血小板活性的影响显示p -选择素浓度显著增加。当肾上腺素激动剂浓度为7.8 mg/mL时，血小板聚集性无显著差异，而浓度越高血小板聚集性越低。ADP和胶原激动剂在浓度为7.8 mg/mL、15.6 mg/mL、31.2 mg/mL和62 mg/mL时，血小板聚集率无显著差异，而浓度高于62.5 mg/mL时，血小板聚集率显著降低。结论hrsl在低浓度下无毒，可增加血小板p选择素，7.8 mg/mL时与肾上腺素激动剂相比不降低血小板聚集，7.8 mg/mL以上时降低血小板聚集。当ADP和胶原激动剂的浓度小于或等于62.5 mg/mL时，它不会降低血小板聚集，但当浓度大于62.5 mg/mL时，它会降低血小板聚集。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Phytochemicals profile, cellular viabillity and platelet activity of Hibiscus rosa-sinensis Linn. leaf extract: An in vitro and ex vivo study

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Phytochemicals profile, cellular viabillity and platelet activity of Hibiscus rosa-sinensis Linn. leaf extract: An in vitro and ex vivo study

Objective

to investigates the phytochemical content of Hibiscus rosa-sinensis Linn leaf extract (HrsL), its toxicity through effects on cell viability, and its impact on platelet activity.

Methods

The cytotoxicity of the leaf extract was evaluated using fibroblast cells, with various concentrations of the extract. The platelet activity of the HrsL, was assessed by treating blood and analysing the concentration of P-selectin and platelet aggregation with the agonists epinephrine, ADP, and collagen, compared to blood without extract treatment as a control.

Results

The H. rosa-sinensis leaf extract contains tannins, polyphenols, alkaloids, and saponins. HrsL was non-toxic at concentrations of 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, 62.5 mg/mL, 125 mg/mL, and 250 mg/mL after 24-h exposure, 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, 62.5 mg/mL, 125 mg/mL, and 250 mg/mL after 48-h exposure, and 15.6 mg/mL and 62.5 mg/mL after 72-h exposure. The effect on platelet activity at 250 mg/mL and 125 mg/mL concentrations of the extract showed a significant increase in P-selectin concentration. Platelet aggregation with the epinephrine agonist did not show significant differences at 7.8 mg/mL, while higher concentrations showed significantly lower aggregation. With the ADP and collagen agonists, no significant differences were observed at concentrations of 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, and 62 mg/mL, whereas concentrations above 62.5 mg/mL showed significantly lower platelet aggregation.

Conclusion

HrsL non-toxic at low concentrations and increases platelet P-selectin, It does not decrease platelet aggregation with the epinephrine agonist at 7.8 mg/mL, but it reduces aggregation at concentrations above 7.8 mg/mL. It does not decrease platelet aggregation with the ADP and collagen agonists at concentrations less than or equal to 62.5 mg/mL, but reduces aggregation at concentrations above 62.5 mg/mL.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.