Gokul Vimal Thangaraj, Ramya Ramadoss, Sandhya Sundar, Suganya Panneer Selvam, Hema Shree K., Nitya K.
{"title":"牙釉质低矿化中无机离子构型分析","authors":"Gokul Vimal Thangaraj, Ramya Ramadoss, Sandhya Sundar, Suganya Panneer Selvam, Hema Shree K., Nitya K.","doi":"10.1016/j.jobcr.2025.07.005","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>Enamel hypomineralization is characterized by a reduction in the concentration of calcium (Ca) and phosphorus (P) in the enamel, along with an increase in carbon (C) concentration. This condition affects the mineral content of the enamel, leading to weakened and more susceptible teeth. These findings highlight the importance of understanding the inorganic ion configuration in enamel hypomineralization for effective restorative approaches and prevention strategies. It can pave the way for better therapeutic interventions. Thus, our study aims to investigate the characteristics of enamel hypoplasia in 5 extracted teeth using FE-SEM and EDX analysis.</div></div><div><h3>Methods</h3><div>The study investigated 5 human teeth with enamel hypoplasia and compared them with 1 normal control tooth. The teeth were cut into sections, dehydrated, and coated with platinum before being analyzed using a JSM-IT800 Field Emission Scanning Electron Microscope (FE-SEM) and Energy Dispersive X-ray Spectroscopy (EDX). After imaging, the specialized software was utilized to analyze and measure enamel hypoplasia's morphological traits and features of enamel hypoplasia. The SEM was used to examine the teeth's morphology, while the EDX was used to analyze the molecules.</div></div><div><h3>Findings</h3><div>Electron microscopic images exhibited altered topography on the surface of hypo-mineralized enamel. Elemental analysis showed the presence of Ca, P, Ag, Mg, C, K, and Cl, and their varied distribution. Normal enamel has 48.3 % oxygen and 27.5 % calcium. In hypoplastic enamel, oxygen increases to 49.1 % and calcium stays at 27.3 %. Phosphorus slightly decreases from 13.5 % to 13.3 %, and carbon decreases from 9.8 % to 8.7 %. There are no significant differences in sodium and chlorine. Enamel hypoplasia is linked to minor changes in elemental composition, with a significant decrease in carbon concentration. Standard deviations indicate the precision of the measurements.</div></div><div><h3>Novelty</h3><div>Prior research on enamel hypomineralization typically relies on comparative approaches, employing SEM and EDX for imaging and analysis. While calcium and phosphorus concentrations are frequently analyzed, the study's inclusion of additional elements and quantitative measurements offers a more comprehensive understanding. Advanced imaging techniques such as FE-SEM allow for detailed analysis of enamel structures. The findings contribute valuable insights to this diverse body of knowledge, crucial due to the complex nature of enamel hypomineralization. Thus, it could provide valuable insights for targeted remineralization techniques, ultimately preventing dental caries development.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 1036-1042"},"PeriodicalIF":0.0000,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of inorganic ion configuration in enamel hypo mineralization\",\"authors\":\"Gokul Vimal Thangaraj, Ramya Ramadoss, Sandhya Sundar, Suganya Panneer Selvam, Hema Shree K., Nitya K.\",\"doi\":\"10.1016/j.jobcr.2025.07.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><div>Enamel hypomineralization is characterized by a reduction in the concentration of calcium (Ca) and phosphorus (P) in the enamel, along with an increase in carbon (C) concentration. This condition affects the mineral content of the enamel, leading to weakened and more susceptible teeth. These findings highlight the importance of understanding the inorganic ion configuration in enamel hypomineralization for effective restorative approaches and prevention strategies. It can pave the way for better therapeutic interventions. Thus, our study aims to investigate the characteristics of enamel hypoplasia in 5 extracted teeth using FE-SEM and EDX analysis.</div></div><div><h3>Methods</h3><div>The study investigated 5 human teeth with enamel hypoplasia and compared them with 1 normal control tooth. The teeth were cut into sections, dehydrated, and coated with platinum before being analyzed using a JSM-IT800 Field Emission Scanning Electron Microscope (FE-SEM) and Energy Dispersive X-ray Spectroscopy (EDX). After imaging, the specialized software was utilized to analyze and measure enamel hypoplasia's morphological traits and features of enamel hypoplasia. The SEM was used to examine the teeth's morphology, while the EDX was used to analyze the molecules.</div></div><div><h3>Findings</h3><div>Electron microscopic images exhibited altered topography on the surface of hypo-mineralized enamel. Elemental analysis showed the presence of Ca, P, Ag, Mg, C, K, and Cl, and their varied distribution. Normal enamel has 48.3 % oxygen and 27.5 % calcium. In hypoplastic enamel, oxygen increases to 49.1 % and calcium stays at 27.3 %. Phosphorus slightly decreases from 13.5 % to 13.3 %, and carbon decreases from 9.8 % to 8.7 %. There are no significant differences in sodium and chlorine. Enamel hypoplasia is linked to minor changes in elemental composition, with a significant decrease in carbon concentration. Standard deviations indicate the precision of the measurements.</div></div><div><h3>Novelty</h3><div>Prior research on enamel hypomineralization typically relies on comparative approaches, employing SEM and EDX for imaging and analysis. While calcium and phosphorus concentrations are frequently analyzed, the study's inclusion of additional elements and quantitative measurements offers a more comprehensive understanding. Advanced imaging techniques such as FE-SEM allow for detailed analysis of enamel structures. The findings contribute valuable insights to this diverse body of knowledge, crucial due to the complex nature of enamel hypomineralization. Thus, it could provide valuable insights for targeted remineralization techniques, ultimately preventing dental caries development.</div></div>\",\"PeriodicalId\":16609,\"journal\":{\"name\":\"Journal of oral biology and craniofacial research\",\"volume\":\"15 5\",\"pages\":\"Pages 1036-1042\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-07-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of oral biology and craniofacial research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212426825001502\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212426825001502","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Analysis of inorganic ion configuration in enamel hypo mineralization
Objectives
Enamel hypomineralization is characterized by a reduction in the concentration of calcium (Ca) and phosphorus (P) in the enamel, along with an increase in carbon (C) concentration. This condition affects the mineral content of the enamel, leading to weakened and more susceptible teeth. These findings highlight the importance of understanding the inorganic ion configuration in enamel hypomineralization for effective restorative approaches and prevention strategies. It can pave the way for better therapeutic interventions. Thus, our study aims to investigate the characteristics of enamel hypoplasia in 5 extracted teeth using FE-SEM and EDX analysis.
Methods
The study investigated 5 human teeth with enamel hypoplasia and compared them with 1 normal control tooth. The teeth were cut into sections, dehydrated, and coated with platinum before being analyzed using a JSM-IT800 Field Emission Scanning Electron Microscope (FE-SEM) and Energy Dispersive X-ray Spectroscopy (EDX). After imaging, the specialized software was utilized to analyze and measure enamel hypoplasia's morphological traits and features of enamel hypoplasia. The SEM was used to examine the teeth's morphology, while the EDX was used to analyze the molecules.
Findings
Electron microscopic images exhibited altered topography on the surface of hypo-mineralized enamel. Elemental analysis showed the presence of Ca, P, Ag, Mg, C, K, and Cl, and their varied distribution. Normal enamel has 48.3 % oxygen and 27.5 % calcium. In hypoplastic enamel, oxygen increases to 49.1 % and calcium stays at 27.3 %. Phosphorus slightly decreases from 13.5 % to 13.3 %, and carbon decreases from 9.8 % to 8.7 %. There are no significant differences in sodium and chlorine. Enamel hypoplasia is linked to minor changes in elemental composition, with a significant decrease in carbon concentration. Standard deviations indicate the precision of the measurements.
Novelty
Prior research on enamel hypomineralization typically relies on comparative approaches, employing SEM and EDX for imaging and analysis. While calcium and phosphorus concentrations are frequently analyzed, the study's inclusion of additional elements and quantitative measurements offers a more comprehensive understanding. Advanced imaging techniques such as FE-SEM allow for detailed analysis of enamel structures. The findings contribute valuable insights to this diverse body of knowledge, crucial due to the complex nature of enamel hypomineralization. Thus, it could provide valuable insights for targeted remineralization techniques, ultimately preventing dental caries development.
期刊介绍:
Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.