Journal of oral biology and craniofacial research最新文献

{"title":"Effect of surface preparation methods on the shear bond strength of universal and self-etch adhesive systems: an in vitro study","authors":"Shiwangi Verma ,&nbsp;Ravishankar Suman ,&nbsp;Rajendra Goud ,&nbsp;Snigdho Das ,&nbsp;Srikant Gollapudi ,&nbsp;Samapika Routray","doi":"10.1016/j.jobcr.2026.101408","DOIUrl":"10.1016/j.jobcr.2026.101408","url":null,"abstract":"<div><h3>Objectives</h3><div>To assess the impact of three smear layer preparation methods on shear bond strength (SBS) to enamel and dentin, using two universal adhesives (Tetric N-Bond Universal, 3M Single Bond Universal) and one two-step self-etch adhesive (Kuraray Clearfil SE Bond).</div></div><div><h3>Methods</h3><div>Ninety extracted premolars were assigned to enamel and dentin groups (n = 45 each) and prepared with coarse grit (120), fine grit (600) silicon carbide paper, or air abrasion (Aluminium Oxide for enamel, Sylc for dentin). Surfaces were bonded with one of the adhesives and restored with composite. Enamel was treated in an etch-and-rinse mode, dentin in self-etch mode. SBS was tested using a universal testing machine. Failure surfaces were examined under SEM to assess fracture morphology and EDS to determine elemental composition. Data were analyzed using two-way ANOVA/Tukey's post-hoc test and Weibull statistics (P ≤ 0.05).</div></div><div><h3>Results</h3><div>In enamel, SBS generally increased from coarse to fine to air abrasion. Kuraray Clearfil SE Bond yielded the highest values, particularly with air abrasion (9.11 ± 0.51 MPa). In dentin, fine grit achieved the highest SBS across all adhesives, followed by air abrasion; coarse grit showed the lowest. Kuraray consistently outperformed the universal adhesives in most dentin conditions. SEM/EDS revealed predominantly adhesive failures; occasional cohesive failures in dentin (Ivoclar) and enamel (Kuraray) corresponded with higher SBS and Weibull reliability.</div></div><div><h3>Conclusion</h3><div>Both surface preparation and adhesive type significantly influence SBS. Kuraray showed superior performance and reliability, while fine grit and air abrasion enhanced bonding efficacy. SEM/EDS findings corroborated the mechanical results.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101408"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regional odontodysplasia: a report of unusual case with in-depth analysis","authors":"Charles Béhot ,&nbsp;Ophélie Cuisinier ,&nbsp;Éden Serraf ,&nbsp;Lisa Friedlander ,&nbsp;Brigitte Vi-Fane ,&nbsp;Pascal Garrec ,&nbsp;Ali Nassif","doi":"10.1016/j.jobcr.2025.12.012","DOIUrl":"10.1016/j.jobcr.2025.12.012","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Regional odontodysplasia (RO) is a rare developmental anomaly affecting dental tissue formation, typically confined to a single quadrant. It manifests through hypoplastic enamel, irregular dentin, short roots with open apices, and a characteristic “ghost-like” radiographic appearance. Its etiology remains unclear, with vascular, genetic and inflammatory factors proposed.</div></div><div><h3>Materials and methods</h3><div>In this study, we report the case of a 15-year-old female with mandibular RO affecting canine, premolars, and molars, focusing on the clinical, radiographic, and microscopic analysis of the affected teeth, including mineral density evaluation.</div></div><div><h3>Results</h3><div>The 2nd premolar and first and second molars (teeth 35, 36, 37) exhibited severe morphological defects and were extracted due to extreme root shortening (40 %, 90 %, and &gt;95 % reduction), while alveolar bone height remained preserved. The canine and 1st premolar were affected to a lesser extent and were conserved upon orthodontic treatment plan. Scanning electron microscopy revealed slight decreases in the number and diameter of the dentinal tubules in the affected teeth compared with those in the control teeth. Contrary to many reported cases of RO, mineral density analysis of enamel and dentin revealed no significant differences between affected and normal teeth. The observations reported in this case suggest that resorptive changes, possibly influenced by inflammatory factors, could contribute to the observed root anomalies. The preservation of alveolar bone height provides a favourable prognosis for prosthetic rehabilitation.</div></div><div><h3>Conclusion</h3><div>These findings help elucidate the structural and functional implications of ROs. This study highlights the importance of early diagnosis and multidisciplinary management for optimal outcomes.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101394"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dimethyl sulfoxide pretreatment on bonding of universal adhesives to pulp chamber dentin","authors":"Yashmin Parveen Karishma,&nbsp;Rajni Nagpal,&nbsp;Udai Pratap Singh,&nbsp;Sakshi Saumya,&nbsp;Shivani Deshwal","doi":"10.1016/j.jobcr.2026.101409","DOIUrl":"10.1016/j.jobcr.2026.101409","url":null,"abstract":"<div><h3>Aim and objective</h3><div>To evaluate the effect of 50 % (v/v) dimethyl sulfoxide (DMSO) pretreatment on immediate (24 h) and delayed (6 months) microtensile bond strength (μTBS) of an etch-and-rinse adhesive and two universal adhesives to dentin.</div></div><div><h3>Materials and methods</h3><div>A total of 144 extracted human molars were decoronated, pulp chambers exposed, and roots sectioned 2 mm apical to the CEJ. Teeth were randomly divided into six experimental groups (n = 24), based on surface pretreatment and adhesive system used: Group 1A: Adper Single Bond 2 (ASB) Group 2A: Tetric N-Bond Universal (TNBU) Group 3A: Prime &amp; Bond Universal (PNBU) Groups 1B, 2B, and 3B received the same adhesives, respectively, following a 50 % (v/v) DMSO wet bonding pretreatment. All adhesives were applied per manufacturer's instructions, followed by composite resin build-up. Ten samples per group were tested for μTBS at 24 h and ten after 6 months of storage. Failure mode was analyzed. Four additional samples per group underwent SEM evaluation to observe the resin–dentin interface.</div></div><div><h3>Statistical analysis</h3><div>Data were analyzed using one-way ANOVA and Tukey's post hoc test for intergroup comparisons. Unpaired t-tests were applied for two-group comparisons. Significance was set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>DMSO pretreatment significantly improved both immediate and long-term μTBS (p &lt; 0.05) across all adhesives. PNBU exhibited the highest bond strength at both time intervals.</div></div><div><h3>Conclusion</h3><div>Dentin pretreatment with 50 % DMSO prior to adhesive application enhances immediate μTBS and preserves bond durability at 6 months, regardless of adhesive system used.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101409"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential effect of Periodontal pathogen Fusobacterium nucleatum and Porphyromonas gingivalis on modulation of specific partial-EMT genes in colorectal cancer cells","authors":"Divya Gopinath ,&nbsp;Balachandar Selvakumar ,&nbsp;Priyadharshini Sekar ,&nbsp;Marwan Mansoor Mohammed ,&nbsp;Zhengrui Li","doi":"10.1016/j.jobcr.2026.101410","DOIUrl":"10.1016/j.jobcr.2026.101410","url":null,"abstract":"<div><h3>Objective</h3><div>While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of <em>Fusobacterium nucleatum</em> (Fn) and <em>Porphyromonas gingivalis</em> (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).</div></div><div><h3>Materials and methods</h3><div>HCT 116 cells were infected with <em>Fn</em> or <em>Pg</em> at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate prognostic significance.</div></div><div><h3>Results</h3><div><em>Pg</em> infection induced a significant upregulation of EMP3 at the 24 h, while suppressing the expression of P4HA2, MXD1, and THBS2. The ZEB2 displayed a biphasic response, decreasing at 6 h before rising at 24 h, and SNAI1 was consistently suppressed. In contrast, <em>Fn.</em> infection resulted in the upregulation of ITGA5 and MMP3, accompanied by the downregulation of P4HA2, MXD1, MMP9, and SNAI1. Both enhanced cellular migration and wound closure capabilities. THBS2, CDH13, and P4HA2 were significantly elevated in tumor tissues compared to controls, while EMP3 and ZEB1 showed reduced expression. Elevated mRNA levels of EMP3, THBS2, and ITGA5 were significantly correlated with poorer overall survival.</div></div><div><h3>Conclusion</h3><div><em>Fn</em> and <em>Pg</em> can promote invasive phenotypes in CRC through distinct mechanisms, each modulating a unique subset of p-EMT and without instigating a complete, coordinated EMT gene signature.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101410"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finite element biomechanical comparison of bone-driven and occlusion-driven fibular flap positioning in lateral segmental mandibular reconstruction","authors":"Dmytro Filonenko ,&nbsp;Yurii Chepurnyi ,&nbsp;Mykola Kryshchuk ,&nbsp;Bekir Osmanov ,&nbsp;Andrii Kopchak","doi":"10.1016/j.jobcr.2026.101419","DOIUrl":"10.1016/j.jobcr.2026.101419","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate, using finite-element analysis (FEA), the stress–strain state of mandibular bone and the bone component of a microvascular free fibula flap (FFF) reconstructing a lateral segmental mandibular defect, comparing two flap positions relevant to implant-supported fixed prosthetic rehabilitation.</div></div><div><h3>Materials and methods</h3><div>CT-derived three-dimensional models of a reconstructed mandible were generated in two geometries: (A) flap aligned with the inferior mandibular border and (B) flap positioned at the level of the alveolar crest. Each geometry received two endosseous implants and a fixed prosthesis and was subjected to representative masticatory loading (vertical/occlusal and anterior/incisal). Four FEA models (2 positions × 2 load cases) were analyzed, with two predefined regions of interest per model (distal and medial), each examined at the upper and lower margins.</div></div><div><h3>Results</h3><div>Compared with Model A (inferior mandibular border), Model B (alveolar crest) produced lower peak bone stresses in most analyzed regions (≈10–40% reduction) under mastication-representative static molar and incisal loads. The only exception was the medial–inferior margin under incisal loading, where stress increased markedly (8.96 vs 2.22 MPa; ∼4-fold). Under occlusal loading, medial–inferior stresses were &lt;1 MPa in both models. Across both positioning scenarios, peak crestal bone von Mises stresses clustered near the implant neck (crestal bone); analyses were restricted to bone.</div></div><div><h3>Conclusion</h3><div>A higher (alveolar-crest) position of the bone component of the FFF yields a more favorable stress–strain environment and load distribution, supporting more favorable biomechanical conditions for osseointegration and long-term function.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101419"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of zinc oxide and ellagic acid on macrophage infiltration, angiogenesis, and collagen deposition in excisional wounds healing","authors":"Intan Nirwana ,&nbsp;Michelle Fidelia Alexandra ,&nbsp;Tarissa Balqis Nuraida ,&nbsp;Ahmad Thariq Bermani ,&nbsp;Yasmin Shahaniz ,&nbsp;Devi Rianti ,&nbsp;Titien Hary Agustantina ,&nbsp;Anita Yuliati ,&nbsp;Khairul Annuar Shariff ,&nbsp;Meircurius Dwi Condro Surboyo","doi":"10.1016/j.jobcr.2026.01.002","DOIUrl":"10.1016/j.jobcr.2026.01.002","url":null,"abstract":"<div><h3>Objectives</h3><div>The combination of zinc oxide (ZnO) and ellagic acid (EA) in wound dressings offers synergistic benefits for wound healing due to their complementary antimicrobial, antioxidant, anti-inflammatory, and tissue regenerative properties. This study aimed to evaluate the effects of ZnO-EA dressing on macrophage infiltration, angiogenesis, and collagen density in excisional wounds on days 3 and 7 post-treatment.</div></div><div><h3>Materials and methods</h3><div>This in vivo posttest-only experimental study involved twenty <em>Rattus norvegicus</em> with excisional dorsal wounds, randomly assigned to two groups: a control group receiving ZnO dressing and a treatment group receiving ZnO-EA dressing. Each group was evaluated at day 3 and day 7, resulting in four observation subgroups. Tissue samples were collected to assess macrophage infiltration and angiogenesis using Hematoxylin and Eosin staining, and collagen density using Masson's Trichrome staining. Histological evaluation was performed under a light microscope at 400× magnification.</div></div><div><h3>Results</h3><div>Treatment with ZnO-EA resulted in significant increase in macrophages infiltration, the number of newly formed blood vessels, and collagen density in the treatment group compared to the ZnO group on both days 3 and 7 (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The application of a ZnO wound dressing enriched with EA significantly enhances macrophage infiltration, angiogenesis, and collagen deposition during the wound healing process.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101398"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145957724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of NLRP3 (rs4612666) polymorphism in gingival crevicular fluid with symptomatic irreversible pulpitis and asymptomatic apical periodontitis","authors":"Saumya Verma ,&nbsp;Dax Abraham ,&nbsp;Vineeta Sharma ,&nbsp;Anjana Goyal ,&nbsp;Alpa Gupta ,&nbsp;Sucheta Jala ,&nbsp;Gurjot Singh ,&nbsp;Rajeev Kumar Malhotra","doi":"10.1016/j.jobcr.2026.101413","DOIUrl":"10.1016/j.jobcr.2026.101413","url":null,"abstract":"<div><h3>Objectives</h3><div>This study investigated the association between the NLRP3 rs4612666 single-nucleotide polymorphism (SNP) and the clinical presentation of symptomatic irreversible pulpitis (SIP) and asymptomatic apical periodontitis (AAP) using gingival crevicular fluid (GCF) samples from a North Indian population.</div></div><div><h3>Methods</h3><div>In this observational case-control study, 48 participants were divided into three groups (n = 16 each): SIP, AAP, and healthy controls. GCF samples were collected for DNA extraction, followed by genotyping of the NLRP3 rs4612666 SNP using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). Genotypic and allelic frequencies were compared across groups, and Hardy–Weinberg equilibrium (HWE) was assessed.</div></div><div><h3>Results</h3><div>The heterozygous TC genotype predominated in both the SIP (62.5 %) and AAP (68.8 %) groups, whereas the control group exclusively exhibited the TT genotype. The CC genotype was absent in all groups. The C allele was more frequent in AAP (43.8 %) than in SIP (18.8 %). Genotypic and allelic distributions differed significantly between the disease groups and controls (p &lt; 0.001). Deviation from HWE in the AAP group (p = 0.0361) suggests potential genetic susceptibility to chronic inflammation.</div></div><div><h3>Conclusion</h3><div>These findings suggest an association between the C allele of the NLRP3 rs4612666 SNP and increased inflammatory response in endodontic disease. Genetic screening may aid in early risk assessment and support personalized treatment planning.</div></div><div><h3>Clinical relevance</h3><div>Identifying NLRP3 rs4612666 polymorphism in patients may help predict susceptibility to chronic pulpal and periapical inflammation. This genetic insight can aid in risk-based diagnosis and guide personalized endodontic treatment planning.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101413"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovine bone graft enhances osteogenic commitment in human osteoblasts via selective suppression of miR-6797-5p: In silico and in vitro study","authors":"Aparna Sethi ,&nbsp;Khushali K Shah ,&nbsp;Dhanraj Ganapathy ,&nbsp;Shahabe Saquib Abullais ,&nbsp;Abdulmajeed Almuaddi ,&nbsp;Saravanan Sekaran","doi":"10.1016/j.jobcr.2026.101424","DOIUrl":"10.1016/j.jobcr.2026.101424","url":null,"abstract":"<div><h3>Background</h3><div>Xenogeneic grafts are widely employed in oral and maxillofacial rehabilitation, but their biological influence extends beyond osteoconduction. Central to osteoblast differentiation is the transcription factor RUNX2, which is tightly regulated by networks of microRNAs (miRNAs). While the osteogenic effects of bovine and porcine grafts have been well studied, little is known about whether ovine bone grafts can alter RUNX2-related post-transcriptional regulation. This study aimed to investigate whether ovine bone graft–derived soluble factors modulate RUNX2-associated miRNAs and thereby influence osteogenic commitment in human osteoblast-lineage cells.</div></div><div><h3>Methods</h3><div>Hydroxyapatite was isolated from ovine femoral bone and used to prepare conditioned medium (OBG-CM). Human osteoblastic cells and mesenchymal stem cells were exposed to OBG-CM for 24 h. Candidate RUNX2-targeting miRNAs were first identified in silico using miRDB and STarMir, with overlapping predictions screened by qRT-PCR. Predicted binding was further analysed by TargetScan. Functional assays were carried out by transfecting cells with hsa-miR-6797-5p mimics and inhibitors, followed by assessment of RUNX2 mRNA and protein expression.</div></div><div><h3>Results</h3><div>Computational analysis highlighted several high-confidence RUNX2-targeting miRNAs, including hsa-miR-6797-5p. Exposure to OBG-CM selectively reduced the expression of hsa-miR-6797-5p, hsa-miR-889-5p and hsa-miR-122-5p, while other candidates showed minimal change. TargetScan identified a conserved 8-mer seed match for hsa-miR-6797-5p within the RUNX2 3′UTR. Transfection with the mimic suppressed RUNX2 expression at both transcript and protein levels, whereas inhibition produced the opposite effect.</div></div><div><h3>Conclusions</h3><div>Ovine bone graft–derived factors can selectively remodel the miRNA landscape in osteoblast-lineage cells. These insights may guide the development of bioactive grafts optimised for post-transcriptional regulation.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101424"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of effectiveness of hyaluronic acid and melatonin with guided tissue regeneration (GTR) membrane versus guided tissue regeneration (GTR) membrane alone in the treatment of intra-bony defects in human subjects: A split-mouth randomised controlled clinical trial","authors":"Subasree Soundarajan,&nbsp;Sankari Malaiappan","doi":"10.1016/j.jobcr.2026.01.001","DOIUrl":"10.1016/j.jobcr.2026.01.001","url":null,"abstract":"<div><h3>Background</h3><div>Melatonin is one substance that is primarily generated by the pineal gland and numerous other tissues and has vital involvement in many physiological processes, including bone remodeling. Over the last several years, another emerging molecule serving as a potential candidate for periodontal regeneration is the hyaluronic acid (HA), a key extracellular matrix component involved in cell migration. The aim of this study is the additive effect of hyaluronic acid and melatonin with GTR membrane in intrabony defects among patients with chronic periodontitis.</div></div><div><h3>Materials and methods</h3><div>15 bilateral intra-bony defects were treated either with Hyaluronic acid and melatonin along with GTR membrane (Group I), or GTR membrane alone (Group II). Clinical and radiographic parameters assessed were pocket depth, clinical attachment level, and volume of bone defect at baseline and 6 months after the intervention. The biochemical parameter assessed was Matrix metalloproteinase - 1 (MMP-1) and Tissue inhibitor of metalloproteinase (TIMP-1) at baseline and one month after the intervention.</div></div><div><h3>Results</h3><div>Both the study groups showed statistically significant differences in all the parameters from baseline to follow-up. However, group 1 (Hyaluronic acid + Melatonin + GTR membrane) showed statistically significant improvement in all the study parameters when compared to group 2 (GTR alone).</div></div><div><h3>Conclusion</h3><div>It can be concluded that the combination of hyaluronic acid and melatonin was effective in the regeneration of intrabony defects among patients with chronic periodontitis. However, further studies with a longer time period and with additional microbiological assessment would be desirable so as to substantiate the current results.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101397"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis between resin-based root canal sealer and recent bioceramic-based root canal sealers using MicroCT, film thickness, and solubility","authors":"Amira Galal Ismail,&nbsp;Manar M. Galal,&nbsp;Tamer M. Hamdy","doi":"10.1016/j.jobcr.2026.01.004","DOIUrl":"10.1016/j.jobcr.2026.01.004","url":null,"abstract":"<div><h3>Background</h3><div>This study is aimed at evaluating the sealing quality of the single cone obturation technique with resin-based root canal sealers (ADseal root canal sealers, control group) and the novel hydraulic bioceramic-based sealers (Fill Root ST and AH Plus Bioceramic sealers). The assessment was performed by micro-CT void analysis. In addition, film thickness and solubility of the sealers were also evaluated.</div></div><div><h3>Materials and methods</h3><div>Voids were evaluated on clear acrylic resin blocks with a standard artificial root canal model using micro-CT. Physical analysis of the sealers assessed film thickness and solubility. Statistical analysis included one-way ANOVA with Tukey's post-hoc test for multiple comparisons and repeated measure ANOVA for related samples.</div></div><div><h3>Results</h3><div>The AH Plus Bioceramic Sealer exhibited the least canal voids (5.70 %), while the Fill Root ST had intermediate voids, and ADseal had the highest (p &lt; 0.001). The coronal third had the most voids, followed by the middle and apical thirds (p &lt; 0.001). AH Plus Bioceramic Sealer had the thinnest film thickness (19.3 μm), followed by Fill Root ST (42.7 μm), and ADseal (81.7 μm) (p &lt; 0.001). In solubility, ADseal had the lowest (0.9 %), Fill Root ST the highest (2.4 %), and AH Plus Bioceramic intermediate (1.5 %) (p &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>The bioceramic-based sealers (Fill Root ST and AH Plus Bioceramic) showed superior performance compared to ADseal in terms of void formation and film thickness, with voids most frequent in the coronal third and least in the apical third.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101400"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}