Journal of oral biology and craniofacial research最新文献

{"title":"Relative stability of Streptococcus mutans gtfB expression following short-term probiotic exposure in mouth-breathing children: a pilot study","authors":"Irma Damayanti Suryana,&nbsp;Putri Kusuma Wardani Mahendra,&nbsp;Anrizandy Narwidina","doi":"10.1016/j.jobcr.2026.101490","DOIUrl":"10.1016/j.jobcr.2026.101490","url":null,"abstract":"<div><h3>Background</h3><div>Mouth-breathing induces alterations in the oral environment that may promote microbial dysbiosis. <em>Streptococcus mutans</em> is a major cariogenic bacterium associated with mouth-breathing conditions, with the <em>gtfB</em> gene representing a key virulence determinant involved in bacterial pathogenicity. However, the molecular response of virulence gene expression under altered respiratory conditions remains insufficiently understood.</div></div><div><h3>Objective</h3><div>This pilot study aimed to investigate the relative stability of <em>gtfB</em> gene expression following short-term probiotic exposure in mouth-breathing children.</div></div><div><h3>Methods</h3><div>A quasi-experimental pre–post study was conducted in a school-based pediatric population<strong>.</strong> Fourteen children aged 9–12 years were recruited, including seven mouth-breathing and seven nasal-breathing controls. Salivary <em>gtfB</em> expression was quantified using real-time PCR and normalized to a housekeeping gene<strong>.</strong> Statistical analyses were performed using the Mann–Whitney and Wilcoxon tests.</div></div><div><h3>Results</h3><div><em>gtfB</em> gene expression was significantly higher in the mouth-breathing group compared with the control group (p &lt; 0.05), indicating increased virulence potential under altered breathing conditions. Following 14 days of <em>Lactobacillus reuteri</em> Prodentis administration, a downward trend in <em>gtfB</em> expression was observed; however, the change did not reach a statistically significant level (p = 0.398), suggesting relative stability of <em>gtfB</em> virulence gene expression.</div></div><div><h3>Conclusion</h3><div>Short-term probiotic exposure demonstrated limited modulation of <em>gtfB</em> expression, indicating relative stability of <em>Streptococcus mutans</em> virulence regulation under mouth-breathing–associated dysbiotic conditions.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101490"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13351142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Deoxypyridinoline levels in gingival crevicular fluid as an alveolar bone loss biomarker in stage II, III periodontitis patients- An observational study","authors":"K. Nivedha,&nbsp;P. Arunmozhi,&nbsp;J. Kavitha,&nbsp;S. Rajasekar,&nbsp;T. Siji Jacob","doi":"10.1016/j.jobcr.2026.101492","DOIUrl":"10.1016/j.jobcr.2026.101492","url":null,"abstract":"<div><h3>Aim</h3><div>To determine the levels of Deoxypyridinoline in the Gingival Crevicular Fluid of systemically healthy subjects, stage II and stage III periodontitis patients and to correlate it with clinical periodontal parameters to test its diagnostic accuracy as a biomarker.</div></div><div><h3>Materials and methods</h3><div>Eighty voluntary participants, aged 25- 55 years, both male and female, were clinically and radiographically examined. They were then allotted into two groups, Group-A (healthy, n = 40) and Group-B (Stage II - Stage III periodontitis, n = 40). GCF samples were collected and stored in Eppendorf tube containing 0.5 ml phosphate buffer at −80C until further use. The samples were thawed and analyed for Deoxypyridinoline levels using a Human Deoxypyridinoline Sandwich ELISA kit.</div></div><div><h3>Results</h3><div>The mean Deoxypyridinoline levels in Group A were 48 ± 12.48 nmol/L and 78 ± 28.45 nmol/L in Group B patients, which was statistically significant (<em>p</em> &lt; 0.001*). A weak positive correlation was found between all clinical periodontal parameters and Deoxypyridinoline levels in periodontitis patients. Receiver Operator Curves which integrated data from Gingival crevicular fluid samples delivered diagnostic accuracy. Area Under Curve was calculated as 0.88 ± 0.04 (<em>p</em> ≤ 0.01*) indicating adequate sensitivity and specificity.</div></div><div><h3>Conclusion</h3><div>Assessing Deoxypyridinoline levels in GCF provides a secure, non-intrusive, and effective biological sample to detect periodontal tissue inflammation and bone loss. Although DPD demonstrates favourable discriminatory ability, the diagnostic threshold values remain exploratory given the cross-sectional design. Longitudinal validation is required before clinical application, and hence DPD can be considered as an adjunctive rather than definitive diagnostic biomarker for periodontal disease.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101492"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148568408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterisation of copper oxide nanoparticle–functionalized corn husk cellulose–PVA membranes for guided tissue regeneration","authors":"Supraja Govindarajan,&nbsp;P.T. Priyangha","doi":"10.1016/j.jobcr.2026.101491","DOIUrl":"10.1016/j.jobcr.2026.101491","url":null,"abstract":"<div><h3>Introduction</h3><div>Guided tissue regeneration (GTR) membranes enable selective cell repopulation, but many existing membranes suffer from limited mechanical stability, rapid degradation, and a lack of sustainable biomaterials. Corn husk, an agricultural waste rich in cellulose, offers an eco-friendly alternative. Polyvinyl alcohol (PVA) reinforcement and copper oxide nanoparticles (CuO NPs) may further enhance strength and biological performance. This study fabricated and in vitro evaluated an electrospun GTR membrane combining corn husk–derived cellulose, PVA, and CuO NPs.</div></div><div><h3>Materials and methods</h3><div>Cellulose was extracted from corn husk through glycerine treatment, alkaline purification, and peroxide bleaching. CuO nanoparticles were synthesised by precipitating Cu(OH)₂ followed by calcination. A composite electrospinning solution of PVA (10 wt%), cellulose (2 wt%), and CuO NPs (5 wt%) was electrospun under optimised conditions (20 kV, 0.5 mL/h, 12 cm). The resulting membrane underwent in vitro characterisation.</div></div><div><h3>Results</h3><div>SEM and EDS confirmed uniform CuO nanoparticle dispersion within the PVA/Cellulose fibres; FTIR verified chemical integration. MTT assay showed &gt;96% cell viability, with small but significant reductions at 1:1 and 1:2 dilutions (p &lt; 0.01). The membrane exhibited hydrophilicity (contact angle ∼19°), tensile stress of 0.43 MPa, and strong antibacterial effects against <em>E. coli</em> (p = 0.0001) and <em>S. aureus</em> (p = 0.00007). Anti-inflammatory and antioxidant activity increased dose-dependently. ALP expression was significantly upregulated (3.20-, 5.40-, and 8.00-fold; p &lt; 0.01), indicating enhanced early osteogenesis.</div></div><div><h3>Conclusion</h3><div>The composite membrane demonstrated excellent biocompatibility, antimicrobial activity, and osteogenic potential, supporting its promise as a sustainable GTR material.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101491"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13355406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-engineered approach using autologous fibroblast-seeded volume-stable collagen matrix for managing gingival recession- A randomized controlled clinical trial","authors":"Devapriyan Sankar,&nbsp;Deepavalli Arumuganainar,&nbsp;Shivakumar Baskaran,&nbsp;Sivaram Gopalakrishnan","doi":"10.1016/j.jobcr.2026.101481","DOIUrl":"10.1016/j.jobcr.2026.101481","url":null,"abstract":"<div><h3>Background</h3><div>Gingival recession is a common deformity, and tissue engineering, using active regeneration, is emerging as a modern approach for management. This study aimed to evaluate the efficacy of autologous gingival fibroblasts seeded onto a volume-stable collagen matrix (VSCM) in treating gingival recession.</div></div><div><h3>Materials and methods</h3><div>A split-mouth randomized controlled clinical trial was conducted on 20 sites in 10 patients presenting with Miller Class I and II gingival recession. Sites were allocated to receive either coronally advanced flap (CAF) with volume-stable collagen matrix (VSCM) (control) or CAF with fibroblast-seeded volume-stable collagen matrix (FVSCM) (test). Autologous gingival fibroblasts were cultured and seeded onto VSCM before transplantation. Plaque index (PI), probing depth (PD), clinical attachment level (CAL), recession depth (RD), recession width (RW), and keratinized tissue width (KTW) were recorded at baseline, 1, 3, and 6 months. Secondary outcomes included Early Healing Score (EHS), Root Coverage Esthetic Score (RCES), and Visual Analog Scale (VAS). Statistical analysis was performed using SPSS (V23) and p &lt; 0.05 was considered significant.</div></div><div><h3>Results</h3><div>Both groups demonstrated significant improvements in clinical parameters over time. The test group showed significantly greater CAL gain and reduction in RD and RW at all follow-up intervals, with sustained outcomes at 6 months. PI and PD remained comparable between groups. Although EHS, RCES, and KTW showed favourable trends in the test group, differences were not statistically significant.</div></div><div><h3>Conclusion</h3><div>Autologous fibroblast-seeded collagen membranes show promise as a biologically driven alternative for managing gingival recession. Further multicentric trials are warranted for long-term validation.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101481"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13293744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of oral health, salivary alterations, and parental KAP in children with congenital heart disease","authors":"Kavita Dhinsa,&nbsp;Brinda Suhas Godhi,&nbsp;Sonali Saha,&nbsp;Ayushi Singh,&nbsp;Arunima Sarkar,&nbsp;Antra Singh","doi":"10.1016/j.jobcr.2026.101504","DOIUrl":"10.1016/j.jobcr.2026.101504","url":null,"abstract":"<div><h3>Background</h3><div>Children with congenital heart disease (CHD) are at high risk for infective endocarditis (IE), with poor oral health being a key portal for bacteraemia. Data holistically integrating clinical status, salivary defence mechanisms, and parental behaviors in this vulnerable population are scarce.</div></div><div><h3>Aim</h3><div>To compare the oral health status, salivary parameters, <em>Streptococcus mutans</em> levels, and parental knowledge-attitude-practice (KAP) between children with CHD at risk for IE and healthy controls.</div></div><div><h3>Materials and methods</h3><div>This cross-sectional comparative study enrolled 65 children with IE-risk CHD and 65 age- and sex-matched healthy controls (aged 3-12 years). Assessments included the DMFT/deft and Simplified Oral Hygiene Index (OHI-S). Unstimulated saliva was analyzed for flow rate, pH, buffering capacity, viscosity, osmolality, and secretory IgA. <em>S. mutans</em> was quantified via culture. Parental KAP was evaluated using a validated questionnaire.</div></div><div><h3>Results</h3><div>The CHD group had significantly higher mean DMFT (4.2 ± 2.1 vs. 1.8 ± 1.5; p &lt; 0.001) and OHI-S scores (2.5 ± 0.7 vs. 1.2 ± 0.5; p &lt; 0.001). They exhibited compromised salivary function (reduced flow, pH, buffering, sIgA; p &lt; 0.05) and elevated pathogenic markers (viscosity, osmolality, <em>S. mutans</em> counts &gt;10⁵ CFU/mL; p &lt; 0.001). Parental KAP scores were significantly lower in the CHD group (p &lt; 0.001). Regression analysis identified CHD status (β = 2.1, p &lt; 0.001), low salivary flow (β = −0.8, p = 0.002), high <em>S. mutans</em> (β = 0.5, p = 0.01), and poor parental practice (β = −0.3, p = 0.03) as key predictors of caries severity.</div></div><div><h3>Conclusion</h3><div>Children with CHD exhibit a deleterious triad of severe caries, dysfunctional salivary defences, and inadequate parental preventive practices. These findings mandate the integration of point-of-care oral screening and structured parent education into routine cardiac follow-up to mitigate IE risk.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101504"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148776857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and characteristics of temporomandibular joint disc displacement based on Magnetic Resonance Imaging (MRI) in pre-clinical diagnosis: A retrospective study","authors":"Bidisha Sarmah,&nbsp;Komal Smriti,&nbsp;Prajna P. Nayak,&nbsp;Ramprasad Vasthare,&nbsp;Manohara A. Bhat","doi":"10.1016/j.jobcr.2026.101494","DOIUrl":"10.1016/j.jobcr.2026.101494","url":null,"abstract":"<div><h3>Introduction</h3><div>Temporomandibular joint (TMJ) is a complex synovial joint commonly affected by temporomandibular disorders (TMDs), with disc displacement being the most prevalent subtype. Magnetic resonance imaging (MRI) is the gold standard for TMJ evaluation, offering superior soft tissue contrast and non-invasive assessment of disc position and morphology.</div></div><div><h3>Aim and objectives</h3><div>To evaluate the diagnostic value of MRI in the preclinical detection of TMJ disc displacement, determine the prevalence of unilateral and bilateral displacement patterns and compare disc position and TMJ anatomical variables between normal and abnormal disc positions in the closed-mouth position.</div></div><div><h3>Methodology</h3><div>This retrospective cross-sectional study analyzed 214 MRI scans of patients aged 13–70 years using high-quality sagittal closed-mouth TMJ sections. Disc thickness, Disc area and Articular eminence inclination (AEI) were assessed by a TMD-trained specialist. Descriptive statistics, Chi-square and Mann-Whitney U tests were applied, with significance set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>After excluding poor-quality scans, 214 participants (428 TMJs; mean age 45.8 years; 61.2% male) were analyzed. Abnormal disc position was present in 61.7% of TMJs, mainly anterior displacement (57.5%) and was associated with increased disc thickness, reduced disc area and flatter AEI (p &lt; 0.001). Symptomatic individuals exhibited thicker discs and reduced AEI (p &lt; 0.05), with no gender differences. Abnormal disc position showed a significant association with TMJ symptoms (p = 0.049).</div></div><div><h3>Conclusion</h3><div>MRI can characterize TMJ disc position and morphology on closed-mouth sagittal sections and frequently identified anterior displacement even in asymptomatic individuals, highlighting its value in early and preclinical diagnosis.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101494"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13380457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148497689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patients’ satisfaction after orthognathic surgery among the Saudi population in the Eastern Province: A cross-sectional study","authors":"Basma AlMaghlouth,&nbsp;Abdullah Alshammari,&nbsp;Zaher Shamma,&nbsp;Laila Hassan Alwusaybie,&nbsp;Abdulrahman Khattar,&nbsp;Intisar A. Siddiqui","doi":"10.1016/j.jobcr.2026.101496","DOIUrl":"10.1016/j.jobcr.2026.101496","url":null,"abstract":"<div><h3>Purpose</h3><div>This study evaluated changes in patient-reported functional, aesthetic, and psychosocial outcomes before and after orthognathic surgery and assessed overall satisfaction among patients treated in the Eastern Province of Saudi Arabia.</div></div><div><h3>Methods</h3><div>A retrospective cross-sectional study was conducted among 100 patients who underwent orthognathic surgery at Dammam Medical Complex, Saudi Arabia. Data were collected through structured telephone interviews using a questionnaire adapted from previously published instruments and translated into Arabic. Paired multi-category outcomes were analyzed using the Stuart-Maxwell test, paired binary outcomes using the McNemar test, and p ≤ 0.05 was considered statistically significant.</div></div><div><h3>Results</h3><div>The sample included 53 females and 47 males, with a mean age of 29.4 ± 5.9 years. Most participants (70%) expected improvement in both aesthetics and chewing function. Overall, 65% reported postoperative improvement in both domains. Significant favorable changes were observed in chewing ability, nasal breathing, facial profile, frontal facial appearance, and smile (all p &lt; 0.001). Self-confidence increased from 50% to 87% (p &lt; 0.001), and 95% said they would recommend surgery to others.</div></div><div><h3>Conclusion</h3><div>Orthognathic surgery was associated with high patient-reported satisfaction and favorable functional, aesthetic, and psychosocial outcomes in this sample from the Eastern Province.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101496"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148776948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthropometric bio-profiling of pulp chamber volume in comparison with Kvaal's method using CBCT images in residential south Indian population","authors":"Blessy Judesly S.,&nbsp;Krithika Chandrasekar Lakshmi,&nbsp;Anuradha Ganesan,&nbsp;Yesoda Aniyan K.","doi":"10.1016/j.jobcr.2026.101489","DOIUrl":"10.1016/j.jobcr.2026.101489","url":null,"abstract":"<div><div>Age and sex estimation are important components of forensic identification, and dental structures provide reliable indicators due to their resistance to post-mortem changes. This study aimed to compare two CBCT-based approaches for forensic assessment in a South Indian population. The primary objective was to estimate age using the modified Kvaal method, while the secondary objective was to assess pulp chamber volume for sex estimation using ITK-SNAP software.</div></div><div><h3>Marterials and methods</h3><div>Study sample includes retrospective analysis of 225 CBCT images collected from June 2020- June 2024 along with patient data to assess chronological age and sex. Maxillary and mandibular canine teeth were specifically selected due to their well-established anatomical features. Pulp chamber volume and linear measurements based on a modified Kvaal method were obtained using ITK-SNAP version 4.0.2. These two methods of morphometric assessment were compared and the accuracy between them were evaluated using necessary statistical analysis.</div></div><div><h3>Results</h3><div>The sample data followed a normal distribution, and both pulp chamber volume and Kvaal's method were significantly associated with age (p &lt; 0.05). Pearson corelation, multiple linear regression analysis revealed that pulp chamber volume was a reliable predictor of age, while logistic regression and ROC curve showed Kvaal's method was more accurate in determining sex.</div></div><div><h3>Conclusion</h3><div>Pulp chamber volume assessment and the modified Kvaal technique are reliable approaches for age estimation. However, pulp volume analysis demonstrates relatively greater efficiency in age estimation, whereas the Kvaal method shows better applicability in sex determination.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101489"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13351132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retreatability of root canals filled with gutta percha and a novel bioceramic sealer- a scanning electron microscopy study","authors":"DSV Sindhuja,&nbsp;Prashant Bhasin,&nbsp;Hemanshi Kumar,&nbsp;Alfredo Iandolo,&nbsp;Dina Abdellatif,&nbsp;Lubna Ahmad,&nbsp;Alfonso Acerra","doi":"10.1016/j.jobcr.2026.101488","DOIUrl":"10.1016/j.jobcr.2026.101488","url":null,"abstract":"<div><h3>Objective</h3><div>To compare effectiveness of 5% acetic acid, 10% formic acid, and chloroform used adjunctively with a rotary retreatment system in removing a premixed calcium-silicate sealer (Ceraseal) and gutta-percha from root canals, using scanning electron microscopy (SEM) to quantify residuals across canal thirds.</div></div><div><h3>Materials and methods</h3><div>Thirty-six extracted single-rooted anterior teeth were instrumented, obturated (single-cone GP + Ceraseal), and stored for two weeks. Specimens were retreated with HyFlex Remover files and randomly assigned (n = 9/group) to: G1—5% acetic acid; G2— 10% formic acid; G3—chloroform; G4—distilled water (negative control). Two milliliters of test solution were used with repeated instrumentation until patency; canals were then irrigated with saline and dried. Roots were split; standardized SEM images (×1000) of coronal, middle, and apical thirds were scored.</div></div><div><h3>Results</h3><div>Intragroup, residuals increased apically in all solvent groups: in G1, apical &gt; middle &gt; coronal (all p = 0.021); in G2, apical &gt; coronal/middle (p = 0.021); in G3, apical &gt; coronal (p = 0.021); G4 showed no significant intragroup differences. Intergroup, coronal third: G1 had significantly lower residuals than G3 and G4; G1 ≈ G2; G2 ≈ G3 ≈ G4. Middle third: G1 and G2 &lt; G4 (p = 0.021); no other pairwise differences. Apical third: no significant between-group differences.</div></div><div><h3>Conclusions</h3><div>When combined with HyFlex Remover files, 5% acetic and 10% formic acids enhanced removal of Ceraseal/gutta-percha, particularly in coronal third; 5% acetic outperformed chloroform coronally. In middle and apical thirds, acidic solvents, chloroform, and water yielded broadly comparable outcomes.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 4","pages":"Article 101488"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13331973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to the Letter to the Editor","authors":"Siriluck Tiranathanagul","doi":"10.1016/j.jobcr.2026.101442","DOIUrl":"10.1016/j.jobcr.2026.101442","url":null,"abstract":"","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 3","pages":"Article 101442"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148231181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}