Journal of oral biology and craniofacial research最新文献

{"title":"Polydopamine-infused toothpaste: An in vitro assessment of cytotoxicity and embryonic toxicology","authors":"Ayesh Das ,&nbsp;Jayashri Prabakar ,&nbsp;I. Meignana Arumugham ,&nbsp;S. Rajeshkumar ,&nbsp;Jayasree Anandan ,&nbsp;Amal Siby","doi":"10.1016/j.jobcr.2025.09.005","DOIUrl":"10.1016/j.jobcr.2025.09.005","url":null,"abstract":"<div><h3>Background</h3><div>Oral health issues affect approximately 3.5 billion individuals globally, with untreated dental caries being the most prevalent condition, as reported by the WHO. While mechanical plaque removal has shown effectiveness in preventing oral diseases, the need for more advanced and bioactive toothpaste formulations persists. Among emerging ingredients, polydopamine (PDA) has shown promising antibacterial and biocompatible properties, making it a potential additive for therapeutic oral care products.</div></div><div><h3>Aim</h3><div>This study aimed to formulate a polydopamine-infused therapeutic toothpaste and evaluate its in vitro cytotoxicity and embryonic toxicity.</div></div><div><h3>Methods</h3><div>The toothpaste was developed using calcium carbonate as an abrasive, glycerin for moisture retention, and carboxymethyl cellulose as a thickening agent. Sodium lauryl sulfate functioned as a foaming agent, and sodium fluoride was included for enamel protection. Xylitol and peppermint oil were added to enhance taste and stability. Polydopamine was synthesized by polymerizing dopamine in an ethanol-water-ammonium hydroxide mixture and incorporated for antimicrobial enhancement. Cytotoxicity was assessed using the Brine Shrimp Lethality Bioassay, exposing <em>Artemia salina</em> nauplii to PDA-formulated toothpaste at concentrations ranging from 10 to 50 μg/mL. Embryonic toxicity was evaluated using the Zebrafish Embryo Toxicity Test over a 78- hour period. Statistical analysis involved Mann-Whitney and Friedman tests.</div></div><div><h3>Results</h3><div>PDA-formulated toothpaste showed significantly lower cytotoxicity than commercial alternatives at all tested concentrations (p &lt; 0.05), with no significant differences in embryonic toxicity. Both formulations exhibited dose-dependent responses (p = 0.001).</div></div><div><h3>Conclusion</h3><div>Polydopamine-infused toothpaste showed reduced cytotoxicity and no embryonic toxicity, indicating its potential as a safe, effective therapeutic oral care additive.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1448-1453"},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of graphene oxide nanoparticle reinforcement on the mechanical properties of maxillofacial silicone","authors":"Vigneshvar Kandaswamy Srinivasan,&nbsp;Naveen Gopi Chander,&nbsp;Muthukumar Balasubramaniam","doi":"10.1016/j.jobcr.2025.09.007","DOIUrl":"10.1016/j.jobcr.2025.09.007","url":null,"abstract":"<div><h3>Background</h3><div>Maxillofacial silicone is commonly used for facial prostheses but suffers from low tensile and tear strength and limited elasticity. The integration of graphene oxide (GO) nanoparticles has been proposed to improve these mechanical shortcomings.</div></div><div><h3>Aim</h3><div>This in vitro study aimed to evaluate the effect of varying GO concentrations (0 %, 0.5 %, 1 %, 2 %, 3 %, and 5 %) on the tensile strength, tear strength, and Shore A hardness of maxillofacial silicone elastomers.</div></div><div><h3>Materials and methods</h3><div>A total of 504 silicone samples were fabricated and divided into six groups based on GO concentration, with 28 samples per group for each test. Silicone and GO were homogenized and cured per standard protocols. Tensile and tear strength were measured using a universal testing machine; Shore A hardness was assessed with a durometer. Material characterization was conducted using FTIR and FESEM.</div></div><div><h3>Results</h3><div>The highest tensile strength (2.253 MPa) was observed in the 3 % GO group (SG-3), indicating superior stress resistance. Tear strength peaked in the 0.5 % GO group (15.625 MPa), with SG-3 showing nearly comparable results (15.184 MPa). Shore A hardness increased slightly with GO content, with SG-3 offering an optimal flexibility-rigidity balance. FESEM confirmed uniform GO dispersion in SG-3, enhancing filler bonding. FTIR revealed increased peak intensities, suggesting improved molecular interactions and a denser polymer matrix.</div></div><div><h3>Conclusion</h3><div>Graphene oxide significantly enhanced the mechanical performance of maxillofacial silicone, with 3 % GO yielding the most favorable combination of strength, elasticity, and material integrity.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1479-1484"},"PeriodicalIF":0.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of masticatory function in the pediatric and adult populations with Treacher Collins syndrome","authors":"Laís Hollara Medeiros ,&nbsp;Patricia Martins Bueno ,&nbsp;Leide Vilma Fidelis da Silva ,&nbsp;Ana Paula Fukushiro ,&nbsp;Ivy Kiemle Trindade-Suedam","doi":"10.1016/j.jobcr.2025.08.033","DOIUrl":"10.1016/j.jobcr.2025.08.033","url":null,"abstract":"<div><h3>Introduction</h3><div>Treacher Collins Syndrome (TCS) is a congenital craniofacial malformation, characterized by zygomatic and mandibular hypoplasia, that may lead to impaired masticatory function.</div></div><div><h3>Objective</h3><div>To characterize the masticatory function of children and adults with TCS in comparison to a control group (CON).</div></div><div><h3>Materials and methods</h3><div>Fifty-four participants were divided into two age- and sex-matched groups: 1) CON: 27 non-syndromic participants (13 children and 14 adults); and 2) TCS: 27 participants with TCS (13 children and 14 adults). Masticatory function was assessed using two tests: 1) Bite Force (BF): using a gnathodynamometer; and 2) Granulometry (GM): size of food particles after 20 masticatory cycles of standardized samples.</div></div><div><h3>Results</h3><div>The mean BF in children with TCS (108.87 ± 48.64 N) was significantly lower than that observed in the CON group (343.97 ± 156.06 N). Similarly, among adults, the BF in the TCS group (233.28 ± 136.83 N) was significantly lower than that of the CON group (412.07 ± 227.62 N). Within the TCS group, children exhibited significantly lower BF than adults, a pattern not observed in the CON group. Children with TCS presented significantly larger particle sizes (0.83 ± 0.86 mm²) than controls (0.26 ± 0.13 mm²). Among adults, no significant differences were observed between groups. Comparisons between age groups showed that children with TCS produced significantly larger particles than adults with TCS.</div></div><div><h3>Conclusion</h3><div>Masticatory function is impaired in participants with TCS, particularly in the pediatric population. The findings underscore the importance of ongoing multidisciplinary clinical and therapeutic care for this population.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1460-1466"},"PeriodicalIF":0.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of botulinum toxin dilution with isolated lidocaine (without epinephrine) on injection pain and post-operative complications: pilot randomized clinical trial","authors":"Farzin Sarkarat ,&nbsp;Marzie Naeini ,&nbsp;Roozbeh Kahali ,&nbsp;Mehdi Sezavar ,&nbsp;Vahid Rakhshan","doi":"10.1016/j.jobcr.2025.09.001","DOIUrl":"10.1016/j.jobcr.2025.09.001","url":null,"abstract":"<div><h3>Introduction</h3><div>Pain and complications of botulinum toxin injection are important deterrents that may reduce patient satisfaction. Toxin attenuation via lidocaine (with or without epinephrine) might alleviate pain. Except a recent study, no study has assessed the effects of the incorporation of lidocaine alone (without epinephrine/additives) to botulinum toxin.</div></div><div><h3>Methods</h3><div>This pilot multicenter double-blind randomized placebo-controlled clinical trial was performed on 102 patients randomly divided into two groups of experimental (lidocaine without epinephrine) or control (normal saline). Patients received 100 units of botulinum toxin. In the experimental group, botulinum toxin was diluted with 1 ml of 2 % lidocaine without adrenalin; in the placebo group, botulinum toxin was diluted with 1 ml of normal saline. Pain was assessed after injection using a 11-point numerical rating scale ‘0–10’. Post-injection complications were assessed 24 h later based on patients' reports and surgeons' observations. Data were analyzed using Student t-test and Fisher exact test (α = 0.05).</div></div><div><h3>Results</h3><div>The groups were balanced in terms of age and gender (<em>P</em> &gt; 0.05). The mean (SD) pain levels in the placebo and lidocaine groups were 3.70 ± 2.05 and 3.39 ± 1.78, respectively (<em>P</em> = 0.310, <em>t</em>-test). Ptosis and diplopia were not observed in any groups. Asymmetry existed in 4 control subjects (8.7 %) and 5 experimental subjects (8.9 %) (<em>P</em> = 1.0, Fisher). In the control group, 12 patients needed retouch treatment (26.1 %); in the lidocaine group, 11 patients needed retouch therapy (19.6 %) (Fisher, <em>P</em> = 0.482).</div></div><div><h3>Conclusions</h3><div>Dilution of botulinum toxin with lidocaine might not reduce injection pain or alter the incidence of the assessed complications after 24 h.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1473-1478"},"PeriodicalIF":0.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of pre and post extraction anxiety levels using anxiety scale among patients visiting department of oral and maxillofacial surgery","authors":"K. Subhikshanaa ,&nbsp;S.P. Indra Kumar ,&nbsp;C. Manjula ,&nbsp;E. Gayathri Priyadharshini ,&nbsp;S. Vaishnavi Prashanth ,&nbsp;Preethi Saraswathi","doi":"10.1016/j.jobcr.2025.09.003","DOIUrl":"10.1016/j.jobcr.2025.09.003","url":null,"abstract":"<div><h3>Background</h3><div>Dental anxiety frequently deters individuals from seeking timely dental care, with extraction procedures often perceived as highly distressing. While general dental anxiety has been studied, few investigations have specifically assessed changes in anxiety before and after dental extraction, particularly among Indian patients and in relation to clinical predictors.</div></div><div><h3>Methods</h3><div>This prospective study enrolled 100 patients (50 males, 50 females; mean age 43.57 ± 13.90 years) scheduled for extraction under local anesthesia. Anxiety was measured using the Dental Fear Survey (DFS) immediately before and after extraction. Additional clinical and psychological parameters—including previous dental experience, pain expectation and experience (VAS), waiting time, use of sedation, and extraction complexity—were documented. Statistical analyses included paired t-tests for pre- and post-extraction DFS scores and correlations with clinical variables. Results tables clearly indicate non-significant (ns) or not analyzed (N/A) parameters.</div></div><div><h3>Results</h3><div>Mean DFS scores decreased significantly post-extraction (61.72 ± 20.24 to 55.08 ± 19.82; mean reduction 6.64 points, ∼10.8 % decrease, p = 0.001), indicating a clinically meaningful decline in anxiety levels. Both genders exhibited reduced anxiety, though females reported slightly higher post-extraction scores. Significant correlations were observed between DFS scores and traumatic dental experience, pain expectation, actual pain experienced, and waiting time (pre-extraction only). Importantly, no significant association was found between DFS scores and extraction complexity or use of sedation, underscoring this as a key finding of the study.</div></div><div><h3>Conclusion</h3><div>Dental extraction under local anesthesia results in immediate anxiety reduction, with gender-related nuances. This study emphasizes the value of perioperative anxiety assessment and identifies predictors that can inform targeted interventions and improvements to current clinical protocols. All results presented correspond strictly to parameters measured and analyzed in this study.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1467-1472"},"PeriodicalIF":0.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of topical ajwain oil in reducing pain during local anaesthesia administration in paediatric dental patients: A randomized controlled trial","authors":"Aatya Takuli ,&nbsp;Akash Bhatnagar ,&nbsp;Praveen Rikhari","doi":"10.1016/j.jobcr.2025.09.008","DOIUrl":"10.1016/j.jobcr.2025.09.008","url":null,"abstract":"<div><h3>Background</h3><div>Needle anxiety is one of the most common barriers to providing stress-free dental care in children. In paediatric dentistry, there is a consistent effort to identify painless techniques for administering local anaesthesia. Topical anaesthetics have long been considered a valuable aid in this regard. Recently, the efficacy of herbal topical anaesthetic agents has been compared with that of conventional sprays and gels. The present study is among the first to evaluate the effectiveness of Trachyspermum ammi (Ajwain) oil as a topical agent for pain control during local anaesthesia administration in paediatric dental patients.</div></div><div><h3>Objective</h3><div>The objective of this study was to evaluate and compare the analgesic efficacy of Ajwain (Trachyspermum ammi) oil with a conventional 15 % w/w lidocaine topical spray in reducing pain perception during the administration of local anaesthesia in paediatric dental patients.</div></div><div><h3>Methods</h3><div>A total of 52 children, aged 6–10 years and meeting the inclusion and exclusion criteria, were randomly divided into two equal groups (Group 1: Ajwain oil; Group 2: topical anaesthetic spray) using the chit method. Pain responses were assessed using the Visual Analogue Scale (VAS) and the Wong-Baker Facial Pain Rating Scale (WBFPRS).</div></div><div><h3>Results</h3><div>Independent t-tests revealed statistically significant differences between Group 1 and Group 2 for both VAS and WBFPRS scores, with p values of 0.001 for each scale, indicating a significant reduction in pain perception in the Ajwain oil group.</div></div><div><h3>Conclusion</h3><div>Ajwain oil shows potential as an effective alternative to conventional topical anaesthetic sprays for reducing injection pain in paediatric dental patients.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1454-1459"},"PeriodicalIF":0.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of the effect of Snoezelen Distraction Technique on children with Autism and healthy children during dental treatment","authors":"Sanjna K. Sreenivasan,&nbsp;Nikita Lolayekar,&nbsp;Kavita Rai,&nbsp;Aishani Baksi,&nbsp;Kripa Dutta,&nbsp;Manju R","doi":"10.1016/j.jobcr.2025.08.022","DOIUrl":"10.1016/j.jobcr.2025.08.022","url":null,"abstract":"<div><h3>Aim</h3><div>To compare the effect of Snoezelen distraction technique (SDT) on children with Autism disorder and healthy uncooperative children during dental treatment.</div></div><div><h3>Method</h3><div>ology: 17 children diagnosed with Autism (Group 1) and 17 uncooperative healthy children (Group 2) requiring dental treatment were considered for the study. The study comprised of two appointments during which physiological and behavioural parameters were recorded. First appointment, dental treatment was done without any distraction and the second appointment with Snoezelen distraction technique (SDT).</div></div><div><h3>Results</h3><div>In Group 1, there was a statistically significant decrease in mean pulse rate (p &lt; 0.01), mean respiratory rate (p &lt; 0.05) and decrease in the negative behaviour frequencies (p &lt; 0.01) from first to second appointment. In Group 2, there was a statistically significant decrease in the mean pulse rate, respiratory rate values (p &lt; 0.01) and decrease in the negative behaviour (p &lt; 0.05) frequencies from first to second appointment. On comparative evaluation, there was a statistically significant difference seen for the values between the groups (p &lt; 0.05) for the change in respiratory rate with higher values in Group 1. In Appointment 2 there was a statistically significant difference seen in the frequencies between the groups for behaviour (p &lt; 0.01) with a higher frequency for negative behaviour in Group 2 and definitively positive in Group 1.</div></div><div><h3>Conclusion</h3><div>In children with Autism and healthy uncooperative children there was a significant change in both physiological and behavioural parameters on using SDT. The changes in these parameters were statistically higher in children with Autism.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1440-1447"},"PeriodicalIF":0.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesoporous Whitlockite: Synthesis, characterization, and in vitro biocompatibility for bone tissue engineering applications","authors":"J.S. Sharukh ,&nbsp;Sinduja Palati ,&nbsp;Saravanan Sekaran ,&nbsp;Dhanraj Ganapathy","doi":"10.1016/j.jobcr.2025.09.002","DOIUrl":"10.1016/j.jobcr.2025.09.002","url":null,"abstract":"<div><h3>Background</h3><div>Whitlockite (WH) is a magnesium-containing calcium phosphate mineral that occurs naturally in bone and teeth. Its biological relevance lies in its ability to promote osteogenesis and provide mechanical stability, making it a strong candidate for bone repair applications. Introducing mesoporosity into Whitlockite is expected to further enhance its biological activity by increasing surface roughness and surface area, which improves protein adsorption and supports cell growth.</div></div><div><h3>Objective</h3><div>This work focused on preparing mesoporous Whitlockite (Meso-Wh) through a controlled acid treatment method, followed by detailed structural and surface characterization, and an in vitro evaluation of its cytocompatibility.</div></div><div><h3>Methods</h3><div>Whitlockite was synthesized using a precipitation–hydrothermal method with calcium nitrate, magnesium nitrate, and diammonium hydrogen phosphate precursors. Mesoporosity was induced by hydrochloric acid treatment (pH 4). The particles were characterized using scanning electron microscopy (SEM) with energy-dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and nitrogen adsorption–desorption studies with BET and BJH analysis. Cytocompatibility was tested by an indirect MTT assay using MG-63 osteoblast-like cells.</div></div><div><h3>Results</h3><div>SEM images showed that Meso-Wh particles were smaller and rougher compared with untreated Whitlockite. EDS confirmed calcium, phosphorus, oxygen, and magnesium as the major elements. XRD patterns indicated reduced crystallinity in Meso-Wh, and FTIR spectra revealed broadening of phosphate bands, suggesting lattice disorder due to acid treatment. BET analysis gave a surface area of 63.07 m²/g, while BJH pore distribution confirmed mesopores mainly in the 2–5 nm range. MTT results showed good cytocompatibility, with high cell viability at 25–75 % extract dilutions and a slight decrease at 100 %. The positive control exhibited marked cytotoxicity.</div></div><div><h3>Conclusion</h3><div>Acid treatment effectively produced mesoporous Whitlockite with enhanced surface area and nanoscale porosity, without altering its chemical composition, indicating its suitability for further development as a bone tissue engineering scaffold.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1433-1439"},"PeriodicalIF":0.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and radiographic success of single-cone bioceramic obturation versus traditional techniques: a systematic review and meta-analysis of randomized controlled trials","authors":"Firas Elmsmari ,&nbsp;Yousef Elsayed ,&nbsp;Abdelrahman Aboubakr ,&nbsp;Mahdi Kaafarani ,&nbsp;Osama Nour ,&nbsp;Ajinkya M. Pawar","doi":"10.1016/j.jobcr.2025.08.031","DOIUrl":"10.1016/j.jobcr.2025.08.031","url":null,"abstract":"<div><h3>Background</h3><div>Bioceramic sealers, known for their bioactivity and biocompatibility, offer a promising alternative to traditional resin-based sealers. However, clinical evaluations are lacking. This systematic review and meta-analysis compared the clinical and radiographic outcomes of bioceramic single-cone obturation with those of conventional treatment and sealers.</div></div><div><h3>Methods</h3><div>We conducted a systematic search of PubMed, ScienceDirect, and the Cochrane Library for randomized controlled trials (RCTs) published up to March 2024. Our review protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO) (<strong>CRD420250212604</strong>). Included studies compared bioceramic single-cone obturation with conventional obturation in permanent teeth with a follow-up of ≥6 months. Risk of bias was assessed using the second version of the Cochrane Risk of Bias tool (RoB 2.0). A random-effects meta-analysis was performed to calculate pooled success rates, odds ratios (ORs), and 95 % confidence intervals (CIs). Heterogeneity and publication bias were assessed via I² statistics and Egger's test.</div></div><div><h3>Results</h3><div>Three RCTs involving 259 participants met inclusion criteria. The success rates for the bioceramic group were 88.7 %, 87.1 %, and 92.0 % at 6, 12, and 18 months, respectively, while those for the controls were 76.4 %, 76.4 %, and 90.7 %, respectively. Three studies reported higher success rates in the bioceramic groups than in the controls, although between-group differences were not statistically significant (overall OR range 1.12–2.09; p &gt; 0.05). We observed moderate heterogeneity at the early follow-ups and negligible heterogeneity at the 18-month follow-ups.</div></div><div><h3>Conclusion</h3><div>Single-cone obturation with bioceramic sealers may result in small but clinically relevant advantages; however, more high-quality RCTs with longer-term follow-up are needed.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1422-1432"},"PeriodicalIF":0.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic acid fillers and botulinum toxin type A: Cross-Sectional analysis for optimizing outcomes based on sex and age","authors":"Ana Paula Pereira da Silva ,&nbsp;Océane Mantel ,&nbsp;Jussara Marinho Dias Frasson ,&nbsp;Mariana Barbosa Câmara-Souza ,&nbsp;Josué Júnior Pierote Araújo ,&nbsp;Renato Assis Machado","doi":"10.1016/j.jobcr.2025.08.029","DOIUrl":"10.1016/j.jobcr.2025.08.029","url":null,"abstract":"<div><h3>Objective</h3><div>To analyze treatment patterns, dosing variations, and reapplication rates of botulinum toxin type A (BoNT-A) and hyaluronic acid injections, with a focus on sex- and age-related differences in outcomes.</div></div><div><h3>Methods</h3><div>A prospective analysis was conducted on 164 patients treated with BoNT-A and/or hyaluronic acid injections. Patients were categorized by sex and age (&lt;50 years or ≥50 years). Data on dosage, treatment regions, and reapplications were collected and analyzed for statistical significance.</div></div><div><h3>Results</h3><div>The study highlighted distinct differences in the application of BoNT-A and hyaluronic acid based on sex and age. For botulinum toxin, the average dose per patient was 55.3 ± 12.7 units. Men received higher doses in the procerus (7.7 ± 2.6 vs.5.7 ± 2.2 units, p = 0.02) and nasal muscles (6.5 ± 2.7 vs.5.3 ± 1.7 units, p = 0.02). Significant age-related differences were observed in the orbicularis oculi (11.0 ± 4.1 vs.14.3 ± 5.4 units, p = 0.02) and corrugator muscles (10.7 ± 3.0 vs.10.5 ± 2.2 units, p = 0.02), with patients ≥50 years requiring higher reapplication doses (p = 0.0001). For hyaluronic acid, men required greater volumes in the mandible (p = 0.0001), reflecting differences in anatomical preferences, whereas women received larger volumes in the lips (p = 0.01), chin (p = 0.02), pre-jowls (p = 0.02), and under-eye regions (p = 0.003), which align with more delicate and aesthetic enhancements.</div></div><div><h3>Conclusion</h3><div>Significant sex- and age-based differences were observed in the application of BoNT-A and hyaluronic acid. Men required higher doses for structural enhancement, while women showed a preference for treatments in aesthetic regions. Patients ≥50 years required higher doses and reapplications, reflecting age-related anatomical changes. These findings highlight the importance of individualized treatment strategies for optimizing outcomes in aesthetic procedures.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1415-1421"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}