Journal of oral biology and craniofacial research最新文献

{"title":"Assisted implant stabilization using a modified microplate fixation technique","authors":"Mohamed Hamdy Helal ,&nbsp;Ahmed Noaman Ali ,&nbsp;Eman Abd El Raouf Tawfik ,&nbsp;Mostafa Mahmoud Youssef Mohamed ,&nbsp;Moustafa Nabil Aboushelib","doi":"10.1016/j.jobcr.2025.06.019","DOIUrl":"10.1016/j.jobcr.2025.06.019","url":null,"abstract":"<div><h3>Objectives</h3><div>In many clinical cases, the surgical defect around the osteotomy does not grant sufficient stability which may compromise the inserted dental implant. Using a fixation microplate may solve this issue by improving the stability of the inserted implant. This study aimed to evaluate assisted implant stability using microplate fixation in compromised bone defects.</div></div><div><h3>Methods</h3><div>Two osteotomy defects (6 mm and 4 mm in diameter) were made in the femur of a dog model. Dental implants (4 mm in diameter and 10 mm in length) were placed in the two defects. Microplates were used to enhance the initial stability of the compromised implant placed in the larger defect size without the need for the additional use of a bone graft. A reverse torque test and histomorphometric analysis were performed after eight weeks to assess bone implant contact (n = 48).</div></div><div><h3>Results</h3><div>Both plate and control implants had almost identical bone-to-implant contact ratios, 82.55 ± 0.61 and 82.86 ± 0.69, respectively. The two tested implants had no statistically significant difference in bone implant contact (t = 1.155, <em>P</em> = .260) nor reverse torque test (t = 1.408, <em>P</em> = .173).</div></div><div><h3>Conclusions</h3><div>Assisted implant stabilization improved osteointegration of implants suffering from poor initial stability without the need to resort to bone graft or other complicated techniques.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 977-981"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of leukocyte-platelet rich fibrin (L-PRF) plug in accelerating the maxillary canine retraction in young adults: A split mouth randomized controlled trial","authors":"A. Iswariya ,&nbsp;Ashok Kumar Jena ,&nbsp;Manaswini Mangaraj ,&nbsp;Jitendra Sharan ,&nbsp;Gautom Kumar Saharia","doi":"10.1016/j.jobcr.2025.06.023","DOIUrl":"10.1016/j.jobcr.2025.06.023","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the effect of the L-PRF plug on the rate of maxillary canine retraction during the comprehensive orthodontic treatment.</div></div><div><h3>Materials and methods</h3><div>The present split-mouth randomized controlled trial included 15 female patients (age range, 18–25 yrs) requiring all 1st premolars extraction for correction of their malocclusion. Right and left sides of the maxillary arch in each patient were randomly allocated to control and experimental sides. Simultaneous canine retraction was initiated immediately after the extraction of 1st premolars on the control side, and placement of L-PRF plugs on the experimental side 1st premolar extraction socket. The amount of canine retraction was evaluated from 3D models of the maxillary study models prepared immediately before (T₀), then at 1 week (T₁), 2 weeks (T₂), 4 weeks (T₃), and 8 weeks (T₄) after the extraction of first premolars and placement of L-PRF. The concentration of IL-6 in the GCF was estimated at T₀, T₁, T₂, T₃, and T₄.</div></div><div><h3>Results</h3><div>Of 15 enrolled, one participant lost during the follow-up. The data of fourteen maxillary arches (14 experimental and 14 control sides) were analyzed. During the first four weeks of observation, the experimental sides had more canine retraction of 0.25 mm (P = 0.001). The mean concentrations of IL-6 at mesial and distal aspects of experimental and control side canines were comparable (P &gt; 0.05). There was a significant increase in IL-6 concentration in the distal compared to the mesial aspects of canine in experimental sides at T₁ (P = 0.004) and T₂ (P = 0.030). There was no correlation between the IL-6 levels and the amount of canine retraction. No adverse effects to any patient were observed during the eight weeks study period.</div></div><div><h3>Conclusion</h3><div>Although the application of L-PRF accelerated the maxillary canine retraction by 0.32 mm throughout the eight weeks, with most of the acceleration occurring in the first four weeks, but it was not clinically significant in decreasing the total duration of orthodontic treatment.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 963-970"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Augmented reality-guided endodontics: Minimally invasive access through the prosthesis using AReneto® system – A case series","authors":"Varun Prabhuji ,&nbsp;Pucha Sai Manaswini ,&nbsp;Srirekha A ,&nbsp;Champa C ,&nbsp;Veena Pai","doi":"10.1016/j.jobcr.2025.06.022","DOIUrl":"10.1016/j.jobcr.2025.06.022","url":null,"abstract":"<div><h3>Introduction</h3><div>Vital tooth preparation for dental prosthesis can lead to apical periodontitis due to factors such as exposed dentinal tubules, poor prosthesis fit, and inadequate oral hygiene. These issues often necessitate root canal treatment. However, removing prosthesis is challenging, particularly when patients hesitate due to cost, complicating treatment decisions. This case series explores the use of the AReneto® system, an augmented reality (AR)-guided approach, for minimally invasive access through existing prosthesis.</div></div><div><h3>Methods</h3><div>Two patients with porcelain-fused-to-metal (PFM) bridge prosthesis presented with pain and periapical pathology. Preoperative virtual planning of the access cavity was performed. The planned access was projected into the clinician's field of view via AReneto® system with the help of the AR glasses to provide guidance during the treatment procedure.</div></div><div><h3>Results</h3><div>The AReneto® system enabled accurate access through the existing prosthesis. The root canals were cleaned, shaped, and obturated successfully. Follow-up appointments showed resolution of symptoms and healing of periapical pathology, confirming the effectiveness of this minimally invasive approach.</div></div><div><h3>Conclusions</h3><div>The AReneto® system allows for precise and minimally invasive endodontic access through existing prosthesis, eliminating the need for prosthesis removal. This reduces the complexity of treatment, enhances efficiency, and ensures successful clinical outcomes.</div></div><div><h3>Statement of clinical relevance</h3><div>The AReneto® system provides an innovative approach that enhances the precision and efficiency of minimally invasive endodontic procedures.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 971-976"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal and restorative integration for managing extreme gummy smiles with lip repositioning flap, crown lengthening, and lithium disilicate veneers: A case report","authors":"Daniyal Lazuardi Ramadhan ,&nbsp;Galih Sampoerno ,&nbsp;Widya Saraswati ,&nbsp;Agung Krismariono ,&nbsp;Tengku Natasha Eleena binti Tengku Ahmad Noor","doi":"10.1016/j.jobcr.2025.06.017","DOIUrl":"10.1016/j.jobcr.2025.06.017","url":null,"abstract":"<div><h3>Background</h3><div>Excessive gingival display, commonly referred to as a gummy smile, has a major impact on facial aesthetics and patient confidence. Addressing this condition often requires a multidisciplinary approach combining periodontal and restorative procedures.</div></div><div><h3>Purpose</h3><div>This case report intends to demonstrate the efficacy of a comprehensive treatment strategy that included a lip repositioning flap, crown lengthening, and lithium disilicate veneers in achieving esthetic rehabilitation of an extreme gummy smile.</div></div><div><h3>Case</h3><div>A 29-year-old male patient presented with excessive gingival display and short clinical crowns, resulting in a disharmonious smile. The patient expressed dissatisfaction with her smile aesthetics and sought a minimally invasive yet effective solution.</div></div><div><h3>Case management</h3><div>The initial evaluation includes clinical and radiographic assessments to identify gingival biotype, crown-to-root ratio, and amount of gingival excess. A lip repositioning flap was performed to optimize gingival levels, followed by crown lengthening to expose adequate tooth structure for veneering. Lithium disilicate veneers were created utilizing a digital approach to improve precision and aesthetics. The treatment lasted six months, which ensured adequate healing and functional integration.</div></div><div><h3>Conclusion</h3><div>The combination of periodontal surgery and advanced restorative techniques successfully addressed the patient's esthetic concerns, achieving a balanced gingival-dental relationship and a natural, harmonious smile. This case demonstrates the value of an interdisciplinary approach in managing complex esthetic challenges, providing predictable and satisfying outcomes for both clinicians and patients.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 955-962"},"PeriodicalIF":0.0,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding success: A five-year retrospective study of dental Implant survival","authors":"Jaideep Mahendra ,&nbsp;Uma Subbiah ,&nbsp;Pavithra GopalaKrishnan ,&nbsp;K. Karunanidhi ,&nbsp;Sathish Rajendran ,&nbsp;Saranya Mohan","doi":"10.1016/j.jobcr.2025.06.021","DOIUrl":"10.1016/j.jobcr.2025.06.021","url":null,"abstract":"<div><h3>Background</h3><div>Dental implants are the preferred option for replacing missing teeth, offering aesthetic and functional benefits. However, factors such as systemic health, smoking, and prosthetic design can influence implant survival. This study aimed to evaluate the impact of clinical, systemic, and prosthetic factors on the survival of dental implants over five years.</div></div><div><h3>Materials and methods</h3><div>This retrospective cohort study included 143 patients who received 161 dental implants between 2016 and 2018, each followed for a minimum of five years. Clinical parameters, systemic conditions and prosthetic factors were evaluated. Radiographic analysis was performed to measure crestal bone loss. Statistical analysis, including multiple regression, was conducted to assess the factors influencing implant survival.</div></div><div><h3>Results</h3><div>The overall implant survival rate was 92.5 %. Factors significantly associated with implant survival included gender (female patients had a higher survival rate, p = 0.01), smoking status (non-smokers had a higher survival rate, p = 0.04), and the absence of parafunctional habits (p = 0.02). Systemic conditions such as diabetes and vitamin D deficiency were also associated with less implant survival rate (p &lt; 0.05). Implant-related parameters, including less crestal bone loss and implant placement protocol type IV (delayed placement) demonstrated statistically significant association with high implant survival. Multiple regression analysis confirmed that females and patients without systemic conditions were significantly more likely to experience implant success.</div></div><div><h3>Conclusion</h3><div>Age, systemic health conditions, smoking status, and prosthetic design significantly influence implant survival. A comprehensive evaluation of these factors is crucial for improving clinical outcomes and optimizing implant success rates.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 948-954"},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144479957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthodontically induced external apical root resorption with clear aligners compared to fixed appliance treatment: An umbrella review","authors":"Madhanraj Selvaraj,&nbsp;Bhaskar Nivethitha,&nbsp;Balasubramanian Madhan","doi":"10.1016/j.jobcr.2025.06.011","DOIUrl":"10.1016/j.jobcr.2025.06.011","url":null,"abstract":"<div><h3>Introduction</h3><div>Orthodontically induced external apical root resorption (OIEARR) is an inevitable complication of orthodontic treatment. This study aimed to provide the existing highest level of evidence on OIEARR in patients undergoing orthodontic treatment with clear aligners compared to fixed orthodontics.</div></div><div><h3>Materials and methods</h3><div>A comprehensive electronic database search was conducted using predefined search terms without any restrictions on language and year of publication. Systematic reviews with or without meta-analysis comparing the OIEARR in fixed and clear aligner groups were only considered. The overlap of primary studies was assessed using the GROOVE tool, and two reviewers graded the quality assessment of included reviews independently using AMSTAR 2. A random effects meta-analysis was carried out for homogenous CBCT data.</div></div><div><h3>Results</h3><div>Out of 1181 articles, eight reviews were eligible; only four considered meta-analyses. A high overlap of 31 primary studies was reported, with 22 included in meta-analyses overlap. AMSTAR 2 tool outlined that the quality of the reviews was critically low in four, with two each being high and low quality. Meta-analysis between the aligner and fixed appliance groups revealed a significant mean difference of 0.62 mm in maxillary central incisors and 0.65 mm in maxillary lateral incisors. The heterogeneity of included primary studies ranged from moderate to high.</div></div><div><h3>Conclusion</h3><div>The incidence and severity of OIEARR are lower in clear aligners than in fixed appliance groups. The results need to be cautiously inferred due to the low to moderate quality of the evidence. Therefore, there is a future need for high-quality RCTs, considering the associated risk factors.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 938-947"},"PeriodicalIF":0.0,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of students’ perspective on introduction of “digital dentistry” as self- directed learning module in undergraduate prosthodontics curriculum: A mixed-method study","authors":"Shuchi Tripathi ,&nbsp;R.K. Dixit ,&nbsp;Suyog Sindhu ,&nbsp;R.D. Singh ,&nbsp;Rameshwari Singhal ,&nbsp;Richa Khanna","doi":"10.1016/j.jobcr.2025.06.010","DOIUrl":"10.1016/j.jobcr.2025.06.010","url":null,"abstract":"<div><h3>Introduction</h3><div>Innovative approaches to curriculum design and implementation are critical in meeting the needs of today's students. The present study was undertaken to determine the perspective of students on the introduced module of ‘Digital Dentistry’ along with knowing their self –directed learning abilities.</div></div><div><h3>Methods</h3><div>The present study was conducted in the Medical University after ethical approval was obtained. Forty volunteers from 3rd and 4th year undergraduates of “Bachelor of Dental Surgery” course were selected and consent form was obtained. A pre-validated questionnaire on self-directed learning (SDLI) was administered to the volunteers to know their SDL abilities. A pre-post-session questionnaire containing feedback questionnaires was prepared, validated and administered before the session. A pre designed module was applied on the volunteers by using SDL session. 2 h duration of First contact session followed by 2 weeks of intersession period, where student went through the shared online/offline study material and visit to the lab under faculty supervision. Outcome assessment was done with 30 MCQ based on the 3 chapters taught in the session. In Second contact session, debriefing by faculty was done and post-module questionnaire was administered. The responses obtained from SDLI, assessment questionnaires and pre-post-module questionnaires were further analyzed statistically.</div></div><div><h3>Results</h3><div>Student's SDLI score with median value of 77.325 represented a good score, with learning motivation scored highest in SDLI. The total score significantly improved post-intervention, with the mean score increasing from 28.83 (5.43) pre-intervention to 35.35 (4.36) post-intervention.</div></div><div><h3>Conclusion</h3><div>Significant improvement in knowledge, awareness and perceptions of digital dentistry was seen after the conduct of the session.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 925-931"},"PeriodicalIF":0.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drilling down to success: Comparative insight on single-drill and sequential-drill techniques for dental implant osseointegration using osteoprotegerin levels - A randomized control trial","authors":"Jerome Rahul James,&nbsp;Karthigeyan Jeyapalan,&nbsp;Shanmuganathan Natarajan","doi":"10.1016/j.jobcr.2025.06.016","DOIUrl":"10.1016/j.jobcr.2025.06.016","url":null,"abstract":"<div><h3>Background</h3><div>To evaluate and compare osteoblastic activity and osseointegration between single-drill and sequential-drill implant site preparation techniques, using osteoprotegerin (OPG) levels as a biomarker.</div></div><div><h3>Methods</h3><div>A randomized controlled trial with a split-mouth design was conducted, involving 12 participants (24 dental implants). The study included two groups: a single-drill group and a sequential-drill group. Gingival crevicular fluid samples were collected at baseline, 15-, 30- and 60-days post-implantation. Osteoprotegerin levels were measured using an enzyme-linked immunosorbent assay (ELISA).</div></div><div><h3>Results</h3><div>Intragroup analysis showed significant variations in OPG levels over time (p &lt; 0.0001). Intergroup comparisons revealed no significant differences at baseline (p = 0.9563) or at 15 days (p = 0.7617); however, significant differences were detected between groups at 30 days (p = 0.0391) and 60 days (p = 0.0005). The single-drill group exhibited higher initial OPG levels, while the sequential-drill group had elevated OPG levels at 30 and 60 days.</div></div><div><h3>Conclusion</h3><div>The single-drill technique may provide an early advantage in osteoblastic response, while the sequential-drill technique supports increased osteoblastic activity at later stages. Both techniques effectively support osseointegration, with selection depending on specific clinical requirements.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 932-937"},"PeriodicalIF":0.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between unilateral-or-bilateral palatal impactions of permanent maxillary canines with dental morphologies, root anatomies, and alveolar measurements","authors":"Alireza Jafari-Naeimi ,&nbsp;Asa Ghorbani ,&nbsp;Farzin Aslani ,&nbsp;Negin Ashoori","doi":"10.1016/j.jobcr.2025.06.012","DOIUrl":"10.1016/j.jobcr.2025.06.012","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the associations between both unilateral/bilateral palatal impactions of the maxillary canine and a long list of various dentoalveolar anatomical measurements and parameters.</div></div><div><h3>Methods</h3><div>This case-control study was performed on CBCTs of 3 groups of 23 subjects each (control, unilateral impaction, bilateral impaction, n = 138 hemimaxillae in 69 patients). Examined were the mesiodistal and buccolingual widths of the 1–6 teeth on the right and left sides, all their roots’ lengths, maxillary arch widths on the axial plane at the basal bone and the alveolar bone levels, at the distances 5, 10, 20, 30, and 40 mm from the most anterior point, and maxillary arch circumferences. Moreover, primary canine retention was assessed as well. The groups were compared using chi-square, <em>t</em>-test, ANOVA, and Tukey tests (α = 0.05).</div></div><div><h3>Results</h3><div>Significant comparisons (<em>P</em> &lt; 0.05) included: At the basal bone level, the arch width of the most anterior section was <em>smaller</em> in the control group compared to the unilateral impaction group only, while interestingly at the alveolar bone level, it was <em>larger</em> in control group compared only to bilateral impaction cases at the most anterior segment. First molar roots were longer in bilateral canine impaction cases compared to control. The buccolingual sizes of premolars/first molar was smaller in control compared to canine impaction. The 5-to-5 arch circumference was smaller in bilateral impaction cases compared the control or unilateral impaction cases (<em>P</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Several dentoalveolar variables were linked to canine impaction.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 911-918"},"PeriodicalIF":0.0,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pandanus amaryllifolius ethanolic extract promotes oral gingival fibroblast healing and inhibits Porphyromonas gingivalis: An in-vitro study","authors":"Monwilun Wilairat,&nbsp;Nattapon Rotpenpian","doi":"10.1016/j.jobcr.2025.06.015","DOIUrl":"10.1016/j.jobcr.2025.06.015","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aimed to evaluate the antimicrobial activity, cytotoxicity and cell migration of ethanolic extract of <em>Pandanus amaryllifolius</em> (PAE).</div></div><div><h3>Methods</h3><div>The antimicrobial activity of PAE was tested against <em>Porphyromonas gingivalis (P. gingivalis</em>) using the microdilution assay and determing minimum inhibitory and bactericidal concentration (MIC and MBC). Additionally, biofilm inhibition was assessed by crystal violet assay at different concentrations of PAE. Moreover, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scratch assays were performed to assess cell viability and migration of human gingival fibroblasts (HGFs).</div></div><div><h3>Results</h3><div>The MIC and MBC values of PAE against <em>P. gingivalis</em> were determined to be 16 mg/mL and 32 mg/mL, respectively. Significant biofilm inhibition was observed at concentrations of 8 mg/mL and 4 mg/mL. The MTT assay demonstrated that 4 mg/mL of PAE was non-toxic to HGFs, whereas higher concentrations exhibited cytotoxic effects. Furthermore, PAE at 4, 2, 1, and 0.5 mg/mL promoted wound healing in an in vitro model.</div></div><div><h3>Conclusion</h3><div>PAE exhibited cytotoxic effects on HGFs at concentrations above 4 mg/mL. However, lower concentrations promoted cell migration and demonstrated potential antimicrobial activity against <em>P. gingivalis</em>. These findings suggest that PAE may have applications in oral health for antimicrobial and wound-healing purposes.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 5","pages":"Pages 919-924"},"PeriodicalIF":0.0,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}