Journal of oral biology and craniofacial research最新文献

{"title":"Pharyngeal airway dimensions and adipose distribution in the minipig","authors":"Doris H. Rosero Salazar ,&nbsp;Riana Grewal ,&nbsp;Anuj Vimawala ,&nbsp;Daniel F. Leotta ,&nbsp;Swati R. Levendovszky ,&nbsp;Zi-Jun Liu","doi":"10.1016/j.jobcr.2024.12.004","DOIUrl":"10.1016/j.jobcr.2024.12.004","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the pharyngeal airway dimensions and regional pharyngeal adipose distribution in the young adult minipig model.</div></div><div><h3>Materials and methods</h3><div>Eight 7-8-months-old Yucatan minipigs, half male and female, were sedated and placed prone to scan the pharyngeal region. Magnetic resonance imaging (MRI) was performed using dynamic turbo-field echo (TFE)-sequence with respiratory gating and adipose-weighted sequence. Respiratory airflow velocity, pressure, and volume were also recorded. The sizes of velopharyngeal and oropharyngeal airway, and retroglossal areas were measured coronally during inspiration and expiration. The airway volumes from the nasal cavity to the retroglossal space were segmented, reconstructed, and evaluated in sagittal views. The adipose distribution in the tongue base, soft palate, pharyngeal wall, tongue body, and masseter muscle (reference) were segmented and measured in sagittal and coronal planes.</div></div><div><h3>Results</h3><div>The velopharyngeal and oropharyngeal areas were larger in inspiration than in expiration. These areas were also larger than that in the retroglossal space (p &lt; 0.05). The nasal cavity showed a larger volume than that of the pharyngeal regions (p &lt; 0.05). The adipose distribution was larger in the posterior region of the tongue base and anterior soft palate, both larger than the masseter muscle (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The larger oropharyngeal dimensions and increased adipose distribution in the tongue base contribute to the functional morphology of the pharyngeal airway in the healthy minipig. These data provide the baseline for further analysis in enlarged and reduced tongue base minipig models.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 77-83"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11714709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of remineralizing potential of four enamel remineralising agents using SEM-EDX – An in-vitro study","authors":"R. Raj","doi":"10.1016/j.jobcr.2025.01.006","DOIUrl":"10.1016/j.jobcr.2025.01.006","url":null,"abstract":"<div><h3>Objective</h3><div>Compare the remineralisation potential of four enamel remineralising agents on artificially demineralized enamel surface using Scanning electron microscopy- Energy dispersive x-ray.</div></div><div><h3>Materials and methods</h3><div>75 extracted maxillary and mandibular premolars coated with acid-resistant nail varnish, were stored in demineralising solution for 96 h to produce artificial caries lesions. The samples were divided into 5 groups (n = 15), Control (Demineralized- No treatment), Group I samples were treated with casein phosphoprotein-amorphous calcium phosphate (CPP-ACP), Group II with self-assembling peptide (SAP-14), Group III with tri-calcium phosphate (f-TCP) and Group IV with Bioactive glass (BAG), respectively. The pH cycling model was followed for 21 days. The samples were analysed via SEM-EDX (Carl Zeiss, Germany; Model: Merlin Compact) for qualitative assessment and quantitative analysis of calcium and phosphorous. The data were analysed for multiple group comparison using IBM SPSS version 20 with one-way ANOVA followed by a paired <em>t</em>-test.</div></div><div><h3>Results</h3><div>Calcium/Phosphorous ratio of all experimental groups; Group 1(1.92 ± .17), Group 2 (1.98 ± .16), Group 3 (1.81 ± .03), Group 4 (1.75 ± .08) was statistically different (p &lt; 0.0005) from Control; while there was no difference between Group I and Group II (p = 0.33).</div></div><div><h3>Conclusion</h3><div>All experimental groups showed comparable remineralising potential. Even though no statistically significant difference is seen between Group I and Group II, after correlating with surface analysis it was concluded that Group II showed the greatest remineralising potential.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 183-187"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental implant placement accuracy with robotic surgery compared to free-hand, static and dynamic computer assisted techniques: Systematic review and meta-analysis","authors":"Hariram Sankar ,&nbsp;M. Shalini ,&nbsp;Anjana Rajagopalan ,&nbsp;Satish Gupta ,&nbsp;Amit Kumar ,&nbsp;Rukhsar Shouket","doi":"10.1016/j.jobcr.2024.12.005","DOIUrl":"10.1016/j.jobcr.2024.12.005","url":null,"abstract":"<div><h3>Background</h3><div>This systematic review and meta-analysis compared the accuracy of robotic-assisted dental implant placement (r-CAIS) with conventional freehand, static computer-assisted (s-CAIS), and dynamic computer-assisted (d-CAIS) techniques.</div></div><div><h3>Methods</h3><div>A comprehensive search was conducted in PubMed, Google Scholar, Semantic Scholar, and the Cochrane Library from January 2000 to January 2024. Studies meeting PICOST criteria, including clinical and in vitro studies, were included. Data on coronal, apical, and angular deviations were extracted for meta-analysis. The risk of bias (RoB) was assessed using the QUIN RoB and JBI RoB tools.</div></div><div><h3>Results</h3><div>A total of 134 models and 100 patients with edentulous and partially edentulous arches were included. Eight studies (four in vitro, four in vivo) were reviewed, demonstrating that r-CAIS offers superior accuracy compared to freehand, s-CAIS, and d-CAIS techniques. Among the studies, two in vitro and two in vivo studies had a low RoB, while others had a high RoB. The meta-analysis of five studies showed significant improvements in coronal, apical, and angular deviations with robotic systems.</div></div><div><h3>Conclusion</h3><div>Robotic-assisted systems showed greater accuracy than traditional non-robotic systems. However, this finding should be interpreted with caution due to the limited number of clinical studies and potential funding biases. Moreover, the high cost of robotic systems presents challenges for routine clinical implementation. Future research should focus on cost-effectiveness and seek broader clinical validation.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 69-76"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11696845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Binaural auditory beats vs music of choice as audio distraction behaviour guidance technique among children: A randomized controlled trial","authors":"Bhuvanesh N. Bhusari,&nbsp;Shivayogi M. Hugar,&nbsp;Niraj Gokhale,&nbsp;Shweta Kajjari,&nbsp;Sanika Karmarkar,&nbsp;Varunika Sahai","doi":"10.1016/j.jobcr.2024.12.019","DOIUrl":"10.1016/j.jobcr.2024.12.019","url":null,"abstract":"<div><h3>Background</h3><div>Binaural beat is created by presenting two different pure-tone sine waves with less than a 30Hz difference dichotically. In dental settings, children listening to familiar music during treatments gain control over the anxiety caused by tools like the airotor or syringe, creating a comforting, familiar environment.</div></div><div><h3>Aim</h3><div>To evaluate and compare anxiety level during restorative treatment using No Music, Music of choice and Binaural Auditory Beats as Audio distraction behaviour guidance technique in children aged 6–12 years.</div></div><div><h3>Methods</h3><div>In-vivo, double-blind, three-arm, parallel-group randomized study was conducted in the department of pediatric and preventive dentistry where 75 participants were allocated into 3 groups. The teeth with Class I cavity in primary and permanent molars were prepared and restored. Anxiety was measured pre- and post-intervention using Pulse oximeter and Venham's picture test.</div></div><div><h3>Results</h3><div>Children who were treated with Binaural beats and Music of choice showed reduction in anxiety levels as indicated by lower anxiety scores in Venham picture test and pulse rate values as compared to the control group.</div></div><div><h3>Conclusion</h3><div>Binaural auditory beats and Music of choice can be used effectively as a non-invasive treatment modality to reduce anxiety in apprehensive pediatric patients.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 163-169"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of exo-long noncoding RNA MALAT1 in OSCC in comparison to dysplastic and normal: A cross-sectional study","authors":"Ramya Sekar ,&nbsp;Selvaraj Jayaraman ,&nbsp;VishnuPriya Veeraraghavan ,&nbsp;Kalaiselvi Krishnamoorthy ,&nbsp;Shubhra Chauhan Aramanai ,&nbsp;Sathan Raj Natarajan","doi":"10.1016/j.jobcr.2024.12.018","DOIUrl":"10.1016/j.jobcr.2024.12.018","url":null,"abstract":"<div><h3>Objective</h3><div>This study explores the role of MALAT1 as a valuable target for creating minimally-invasive diagnostic methods and personalized treatments in the management of OSCC. It focuses on evaluating the role of exosomal MALAT1 in the progression of dysplasia to OSCC by influencing the PI3K/AKT pathway.</div></div><div><h3>Method</h3><div>This cross-sectional study evaluated MALAT1 expression and PI3K/AKT pathway components in exosomes derived from plasma samples of patients with various stages of oral dysplasia, OSCC and compared with normal. RNA concentration was estimated, real-time polymerase chain reaction (qPCR) was used for quantitative analysis. Gene expression levels of MALAT1, PI3K, AKT1, and PTEN were analysed and compared across groups using one way ANOVA and Post-hoc Tukey analysis was performed for pairwise comparisons to assess correlations between MALAT1 expression and PI3K/AKT pathway components.</div></div><div><h3>Result</h3><div>MALAT1 was found to be overexpressed in OSCC in comparison to normal, significantly (<em>p</em> &lt; 0.001∗). There was no significant change in expression pattern of MALAT1 between dysplastic patients and normal, yet, significant association was found on corelation analysis between expression pattern of MALAT1 and PI3K/AKT/PTEN (<em>p</em> 0.001∗) among individuals of dysplasia and OSCC. As well pairwise comparisons of MALAT1 expression levels between all three stages of dysplasia showed significant association (<em>p</em> &lt; 0.001∗).</div></div><div><h3>Conclusion</h3><div>MALAT1 stands out as a key player in the complex landscape of OSCC pathogenesis, impacting tumorigenesis, metastasis, and treatment outcomes through multifaceted molecular mechanisms. Continued research into MALAT1's regulatory roles and its interactions within the tumor microenvironment holds promise for uncovering novel therapeutic targets and biomarkers that could redefine the management of OSCC in the future.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 123-128"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of patient comfort and satisfaction after the surgical removal of mandibular impacted molars using a piezo-electric technique: A randomized double-blind clinical trial","authors":"Khalid Zabin Alotaibi ,&nbsp;Waleed Mohammed Al Rasheed ,&nbsp;Jiingade Krishnojirao Dayashankara Rao ,&nbsp;Abdulrahman Altwaijri ,&nbsp;Gharam Mefleh Alharbi ,&nbsp;Shatha saleh Aljaber","doi":"10.1016/j.jobcr.2024.12.009","DOIUrl":"10.1016/j.jobcr.2024.12.009","url":null,"abstract":"<div><h3>Introduction</h3><div>This is a double clinical trial conducted to study the patient satisfaction and comfort during and after surgical removal of impacted mandibular 3rd molar using Piezo electric tips.</div></div><div><h3>Material and methods</h3><div>Two groups of 25 each [14 (56 %) males and 11 (44 %) females] and [18 (72 % males and 07 (28 %) females] represented the Rotary and Piezo groups respectively with bilateral impactions. one side of the patient is operated by piezo and the other side by rotary instrument after a gap of 2–3 week between the procedures. The variables considered are pain, swelling, trismus, patient satisfaction and comfort. The readings were taken on base line,3rd day,7th and 14th day.</div></div><div><h3>Results</h3><div>Descriptive statistical analysis was performed using means and standard deviations for continuous variables and percentages for categorical variables. The level of significance was set at p &lt; 0.05. The surgery time taken was more in the Piezo group (26.96 ± 12.201) in comparison with the Rotary group (21.32 ± 12.786- (p = 0.011∗). Satisfaction was rated better in the Piezo group (3.18 ± 0.909 vs 2.88 ± 0.881) (p = 0.013∗). Tactile sensation was less in the Piezo group (1.24 ± 0.436) vs (2.28 ± 0.678) (p = 0.001). Less pain was reported in the piezo group patients and the difference between the two groups was statistically significant only during the third day follow up (p = 0.001).</div></div><div><h3>Conclusion</h3><div>The overall patient satisfaction and comfort was better in piezo in spite of longer duration of surgery. It provides better quality of life as the patients experienced less pain and the total number of analgesics taken is significantly less compared to rotary group.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 108-112"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of nanoemulsion carbonated hydroxyapatite-statin administration on Acp 5 and Runx-2 expression during orthodontic relapse in rats","authors":"Ananto Ali Alhasyimi ,&nbsp;Niswati Fathmah Rosyida ,&nbsp;Ika Dewi Ana","doi":"10.1016/j.jobcr.2024.12.013","DOIUrl":"10.1016/j.jobcr.2024.12.013","url":null,"abstract":"<div><h3>Background</h3><div>Retention is an essential element of orthodontic treatment. In the past two decades, numerous biological treatments have been developed to alleviate orthodontic relapse. Pharmacologic bone modulation is a viable approach to mitigate relapse. The aim of this investigation was to determine whether administering nanoemulsion carbonated hydroxyapatite-statin (CHA-statin) would increase Runx-2 expression and decrease Acp 5 expression in rats experiencing experimental orthodontic relapse.</div></div><div><h3>Materials &amp; methods</h3><div>Forty-eight rats (n = 48) were assigned to four groups: control, CHA, statin, and CHA-statin, with 12 rats in each group. A 30 g mesial traction was applied for 7 days via a closed-coil spring that connected the first maxillary molar to the maxillary incisor. To maintain the moved teeth, CHA, statin hydrogel, and nanoemulsion CHA-statin were administered intrasulcularly every 3 days for a period of 7 days. The removal of the devices facilitated the occurrence of relapse. The expression of Acp 5 and Runx-2 was evaluated using the immunohistochemistry method. The collected data were evaluated using analysis of variance and post hoc tests, with <em>p</em> &lt; 0.05.</div></div><div><h3>Results</h3><div>A significant reduction in Acp 5-positive cells in the CHA-statin group was observed on days 7 and 14 of relapse movement compared with the other groups (<em>p</em> &lt; 0.05), whereas the average Runx-2 expressions in the CHA-statin groups were significantly higher than in the other groups on days 1, 7, and 14 during the relapse phase (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The nanoemulsion CHA-statin increased Runx-2 expression and decreased Acp 5 expression, thereby potentially preventing orthodontic relapse in rats.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 129-135"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers","authors":"Marcos Coelho Santiago ,&nbsp;Gustavo Henrique de Oliveira Salles ,&nbsp;Gustavo Gomes de Lima ,&nbsp;Laudimar Alves de Oliveira ,&nbsp;Loise Pedrosa Salles","doi":"10.1016/j.jobcr.2025.01.008","DOIUrl":"10.1016/j.jobcr.2025.01.008","url":null,"abstract":"<div><h3>Background</h3><div>The present study aimed to compare the viability, proliferation, and mineralization potential of human dental pulp cells (hDPCs) and osteoblasts cell line (Saos-2) after exposure to AH Plus® Bioceramic (AHP-B), Bio-C® Sealer (BIO-C), NeoMTA Plus® (NEOMTA-P), and MTA-FILLAPEX® endodontic sealers (MTA-F).</div></div><div><h3>Methods</h3><div>All materials were prepared according to the manufacturer's instructions. Before exposing the cells, we measured the release of calcium ions (Ca²⁺) from the dental materials to the culture media once Ca²⁺ can trigger signaling pathways. After that, hDPCs and Saos-2 were exposed to the sealers for MTT assay to assess the cell viability and wound healing to evaluate the cell proliferation. To investigate the potential of mineralization, we assessed the alkaline phosphatase activity and calcium deposition by Alizarin red staining. Statistical analysis was performed using Two-way ANOVA for calcium release and wound healing assays and One-way ANOVA for other assays, with post-test Bonferroni correction. The results were significant when p &lt; 0.05.</div></div><div><h3>Results</h3><div>The sealers released diverse concentrations of calcium at different times. The hDPCs viability and proliferation were low in the AHP-B group at 24h of exposure (NEOMTA-P ∼ BIO-C ∼ CT &gt; AHP-B &gt; MTA-F), distinct from the osteoblastic cells (NEOMTA-P ∼ AHP-B ∼ CT &gt; BIO-C &gt; MTA-F) and (proliferation: AHP-B &gt; NEOMTA-P ∼ CT &gt; BIO-C &gt; MTA-F). The ALP activity, an early marker of osteogenesis, was higher in hDPCs exposed to NEOMTA-P, while the osteoblastic cells showed higher ALP when exposed to AHP-B.</div></div><div><h3>Conclusion</h3><div>AHP-B, NEOMTA-P, and BIO-C stimulated osteogenesis in hDPCs and Saos-2 cells, with marked differences between groups. AHP-B showed an improved early stimulation of osteoblastic cells, while hDPCs were more responsive to NEOMTA-P.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 191-198"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes","authors":"Marcelo Germani ,&nbsp;Márcia V.G.B. de Queiroz ,&nbsp;Mariana Yuri de França Shimizu ,&nbsp;Thiago Gomes Teixeira ,&nbsp;Victor Rogerio ,&nbsp;Gabriela Giro ,&nbsp;Victor R.M. Munoz-Lora","doi":"10.1016/j.jobcr.2025.01.001","DOIUrl":"10.1016/j.jobcr.2025.01.001","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to compare the in-vitro behavior of four Hyaluronic acid (HA) gels when exposed to two different hyaluronidase (HSE) formulations.</div></div><div><h3>Methods</h3><div>Four commercially available HA were used: Lyft (Lt; Restylane, Galderma, Sweden), Voluma (Vol; Allergan, AbbVie, USA), UltraDeep (UDe; Rennova, Innovapharma, Brazil), and Subskin (Skn; Perfectha, Sinclair, France). The gels were divided into two groups, with seven aliquots (0.1 mL per aliquot) for each group deposited on a glass plate. A millimeter ruler was positioned behind the plate to measure the gel height. Each aliquot received 100 UTR of one of two HSE formulations. After 2 min, each aliquot was mixed using a 22G needle and left to sit for additional 2 min.</div></div><div><h3>Results</h3><div>Partial and final degradation of the products were obtained through photographic analysis. The final percentage of degradation exhibited significant differences among HA gels (P &lt; 0.001). The results revealed higher degradation in Lt, moderate degradation in UDe, and lower degradation in Vol and Skn. Comparisons of HSE formulations showed no significant differences among them (P = 0.881). Moreover, there was a noticeable degradation after mixing (P &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>Within the limits of this study, it can be suggested that Lt exhibits the higher degradation among the experimented gels. Furthermore, differences among HSE formulations do not appear to significantly impact HA degradation, while the mixing movement of HSE and HA seems to influence the degradation rate. These findings may help guide clinical decisions regarding the use of hyaluronidase in managing HA filler complications or adjustments.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 178-182"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the effect of virgin coconut oil pulling on viral load, bacterial load and inflammatory mediator levels in chronic periodontitis – A clinical study","authors":"Monika M ,&nbsp;Rudhra K ,&nbsp;Vennila K ,&nbsp;Elanchezhiyan ,&nbsp;Sri Harini P ,&nbsp;S. Shakeron","doi":"10.1016/j.jobcr.2025.01.004","DOIUrl":"10.1016/j.jobcr.2025.01.004","url":null,"abstract":"<div><h3>Background and objective</h3><div>Periodontitis and dental caries are among the most prevalent oral diseases, with chronic periodontitis being a multifactorial, infectious condition that leads to inflammation in the supporting structures of the teeth, progressive attachment loss, and bone resorption. Chronic periodontitis is driven by a consortium of pathogenic microorganisms. This study aimed to evaluate the efficacy of virgin coconut oil (VCO) pulling in reducing the microbial load and inflammatory mediators responsible for chronic periodontitis, in comparison to chlorhexidine (CHX) mouthwash and distilled water.</div></div><div><h3>Methods</h3><div>Thirty patients diagnosed with chronic periodontitis were randomly allocated into three groups. Group A served as the control and used distilled water for oral rinsing, Group B used chlorhexidine mouthwash, and Group C performed oil pulling with virgin coconut oil. Pre-operative subgingival plaque samples were collected from all participants, followed by complete scaling and root planing. Post-operative samples were collected after four weeks. Both pre- and post-treatment samples were subjected to real-time PCR (rtPCR) and enzyme-linked immunosorbent assay (ELISA) to quantify viral and bacterial loads, as well as levels of key inflammatory mediators.</div></div><div><h3>Results</h3><div>A significant reduction in viral load, bacterial load, and inflammatory mediators was observed in both the VCO and CHX groups compared to the distilled water group. The reductions in the VCO and CHX groups were statistically significant, with comparable efficacy between the two interventions.</div></div><div><h3>Conclusion</h3><div>Virgin coconut oil pulling demonstrated a significant reduction in microbial and inflammatory markers in patients with chronic periodontitis, showing similar efficacy to chlorhexidine mouthwash. Given its comparable therapeutic effects and reduced side effect profile, VCO presents a viable alternative to chlorhexidine for managing chronic periodontitis.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 1","pages":"Pages 153-158"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}