Comparative efficacy of LLLT and LED therapy on osteoblast proliferation and osteoclast suppression: An in vitro co-culture study

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-02-11 DOI:10.1016/j.jobcr.2025.01.018

Dr Divyabharathi Selvam, Dr Venkat Rengasamy

{"title":"Comparative efficacy of LLLT and LED therapy on osteoblast proliferation and osteoclast suppression: An in vitro co-culture study","authors":"Dr Divyabharathi Selvam, Dr Venkat Rengasamy","doi":"10.1016/j.jobcr.2025.01.018","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Dental implants are a reliable solution for tooth loss, but challenges remain regarding osseointegration and complications. Photobiomodulation therapy (PBMT), including Low-Level Laser Therapy (LLLT) and Light Emitting Diode (LED) therapy, has shown potential in enhancing bone healing. However, comparative studies on their effects on osteoblasts and osteoclasts are limited.</div></div><div><h3>Aim</h3><div>To compare the effects of LLLT and LED therapy on osteoblast proliferation and osteoclast suppression in an in vitro co-culture model.</div></div><div><h3>Materials and methods</h3><div>MG-63 osteoblast-like cells and RAW 264.7 osteoclasts were co-cultured on titanium discs. Discs were treated with LLLT (940 nm, 50 mW) or LED (660 nm, 89 mW) for different durations. Osteoblast proliferation was measured by MTT assay, and osteoclast activity was evaluated using TRAP staining and resorption pit analysis.</div></div><div><h3>Results</h3><div>LED therapy significantly enhanced osteoblast proliferation and suppressed osteoclast activity more effectively than LLLT. Optimal results were observed with a 6-min LED exposure.</div></div><div><h3>Conclusion</h3><div>LED therapy shows greater potential than LLLT for promoting osteoblast proliferation and suppressing osteoclast activity, indicating its clinical value for improving bone regeneration and implant integration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 325-330"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221242682500020X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Dental implants are a reliable solution for tooth loss, but challenges remain regarding osseointegration and complications. Photobiomodulation therapy (PBMT), including Low-Level Laser Therapy (LLLT) and Light Emitting Diode (LED) therapy, has shown potential in enhancing bone healing. However, comparative studies on their effects on osteoblasts and osteoclasts are limited.

Aim

To compare the effects of LLLT and LED therapy on osteoblast proliferation and osteoclast suppression in an in vitro co-culture model.

Materials and methods

MG-63 osteoblast-like cells and RAW 264.7 osteoclasts were co-cultured on titanium discs. Discs were treated with LLLT (940 nm, 50 mW) or LED (660 nm, 89 mW) for different durations. Osteoblast proliferation was measured by MTT assay, and osteoclast activity was evaluated using TRAP staining and resorption pit analysis.

Results

LED therapy significantly enhanced osteoblast proliferation and suppressed osteoclast activity more effectively than LLLT. Optimal results were observed with a 6-min LED exposure.

Conclusion

LED therapy shows greater potential than LLLT for promoting osteoblast proliferation and suppressing osteoclast activity, indicating its clinical value for improving bone regeneration and implant integration.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.