重度抑郁症患者脱落口腔黏膜细胞的氧化改变

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-02-10 DOI:10.1016/j.jobcr.2025.01.026

Lukas Mendes de Abreu , Cintia Rodrigues da Silva , Ana Laura Ferreira Bortoleto , Giovana Barros Nunes , Matheus Martins Gracia , Rafael Akira Tzanno Murayama , Daniel Galera Bernabé , Gisele Zoccal Mingoti

{"title":"重度抑郁症患者脱落口腔黏膜细胞的氧化改变","authors":"Lukas Mendes de Abreu , Cintia Rodrigues da Silva , Ana Laura Ferreira Bortoleto , Giovana Barros Nunes , Matheus Martins Gracia , Rafael Akira Tzanno Murayama , Daniel Galera Bernabé , Gisele Zoccal Mingoti","doi":"10.1016/j.jobcr.2025.01.026","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to investigate oxidative stress markers in the oral mucosal cells of individuals diagnosed with major depressive disorder (MDD).</div></div><div><h3>Methods</h3><div>A case-control design was used, including twenty patients diagnosed with MDD, based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) criteria, and twenty healthy controls. Oral exfoliated cells were collected from all participants. Intracellular levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), caspase-3 and -7 activity, and reduced glutathione (GSH) were measured in Arbitrary Fluorescence Units (AFU).</div></div><div><h3>Results</h3><div>The MDD group demonstrated significantly elevated intracellular ROS levels (p = 0.0012) and caspase-3 and -7 activity (p = 0.0171) in comparison to the control group. Additionally, a decrease in ΔΨm expression was observed in the oral cells of MDD patients (p = 0.0265), whereas GSH expression levels did not differ significantly between the two groups (p = 0.8908).</div></div><div><h3>Conclusions</h3><div>The findings indicate heightened oxidative stress in the oral exfoliated cells of individuals with MDD. This study supports the potential use of oral cells as a non-invasive biomarker source for assessing oxidative stress in depressive disorders.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 256-261"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder\",\"authors\":\"Lukas Mendes de Abreu , Cintia Rodrigues da Silva , Ana Laura Ferreira Bortoleto , Giovana Barros Nunes , Matheus Martins Gracia , Rafael Akira Tzanno Murayama , Daniel Galera Bernabé , Gisele Zoccal Mingoti\",\"doi\":\"10.1016/j.jobcr.2025.01.026\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><div>This study aimed to investigate oxidative stress markers in the oral mucosal cells of individuals diagnosed with major depressive disorder (MDD).</div></div><div><h3>Methods</h3><div>A case-control design was used, including twenty patients diagnosed with MDD, based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) criteria, and twenty healthy controls. Oral exfoliated cells were collected from all participants. Intracellular levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), caspase-3 and -7 activity, and reduced glutathione (GSH) were measured in Arbitrary Fluorescence Units (AFU).</div></div><div><h3>Results</h3><div>The MDD group demonstrated significantly elevated intracellular ROS levels (p = 0.0012) and caspase-3 and -7 activity (p = 0.0171) in comparison to the control group. Additionally, a decrease in ΔΨm expression was observed in the oral cells of MDD patients (p = 0.0265), whereas GSH expression levels did not differ significantly between the two groups (p = 0.8908).</div></div><div><h3>Conclusions</h3><div>The findings indicate heightened oxidative stress in the oral exfoliated cells of individuals with MDD. This study supports the potential use of oral cells as a non-invasive biomarker source for assessing oxidative stress in depressive disorders.</div></div>\",\"PeriodicalId\":16609,\"journal\":{\"name\":\"Journal of oral biology and craniofacial research\",\"volume\":\"15 2\",\"pages\":\"Pages 256-261\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-02-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of oral biology and craniofacial research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212426825000284\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212426825000284","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

摘要

目的探讨重度抑郁症（MDD）患者口腔黏膜细胞氧化应激标志物的变化。方法采用病例对照设计，选取20例根据《精神障碍诊断与统计手册》（DSM-5）诊断为重度抑郁症的患者和20例健康对照。收集所有参与者的口腔脱落细胞。用任意荧光单位（AFU）测定细胞内活性氧（ROS）水平、线粒体膜电位（ΔΨm）、caspase-3和-7活性以及还原性谷胱甘肽（GSH）。结果与对照组相比，MDD组细胞内ROS水平（p = 0.0012）和caspase-3、-7活性（p = 0.0171）显著升高。此外，在MDD患者口腔细胞中观察到ΔΨm表达降低（p = 0.0265），而GSH表达水平在两组之间无显著差异（p = 0.8908）。结论MDD患者口腔脱落细胞氧化应激升高。这项研究支持了口腔细胞作为评估抑郁症氧化应激的非侵入性生物标志物来源的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder

查看原文本刊更多论文

Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder

Objectives

This study aimed to investigate oxidative stress markers in the oral mucosal cells of individuals diagnosed with major depressive disorder (MDD).

Methods

A case-control design was used, including twenty patients diagnosed with MDD, based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) criteria, and twenty healthy controls. Oral exfoliated cells were collected from all participants. Intracellular levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), caspase-3 and -7 activity, and reduced glutathione (GSH) were measured in Arbitrary Fluorescence Units (AFU).

Results

The MDD group demonstrated significantly elevated intracellular ROS levels (p = 0.0012) and caspase-3 and -7 activity (p = 0.0171) in comparison to the control group. Additionally, a decrease in ΔΨm expression was observed in the oral cells of MDD patients (p = 0.0265), whereas GSH expression levels did not differ significantly between the two groups (p = 0.8908).

Conclusions

The findings indicate heightened oxidative stress in the oral exfoliated cells of individuals with MDD. This study supports the potential use of oral cells as a non-invasive biomarker source for assessing oxidative stress in depressive disorders.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.