Bio-functional azithromycin loaded polyvinyl alcohol and fucoidan hydrogel coating for titanium Implants: An experimental in vitro study

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-07-21 DOI:10.1016/j.jobcr.2025.07.011

Maria Sharon Velraj, Arvina Rajasekar

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引用次数: 0

Abstract

Background

Titanium-based implants are widely used in biomedical applications due to their superior mechanical properties and biocompatibility. However, the risk of postoperative infections and inadequate osseointegration remains a significant challenge. Hydrogels, with their tunable properties, offer a promising approach for enhancing the bioactivity of titanium surfaces. This study explored the development and characterization of an azithromycin-loaded PVA-fucoidan hydrogel coating on titanium and evaluated its physicochemical and biological properties.

Aim

To fabricate and characterize an azithromycin-loaded PVA-fucoidan hydrogel coated on titanium surfaces and assess their antibacterial, hemocompatibility, and biocompatibility properties.

Materials and methods

Commercially pure ASTM grade V titanium disks (n = 25) were prepared and treated via radio frequency argon plasma. PVA-fucoidan hydrogel was synthesized and loaded with azithromycin (1 % w/w), followed by electrophoretic deposition onto the titanium surface. Surface characterization was performed using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), and contact angle measurements. In-vitro drug release was analyzed using UV–Vis spectrophotometry. Antibacterial efficacy was tested against Streptococcus mutans and Escherichia coli (n = 3 per group). Hemocompatibility and cytotoxicity were evaluated via hemolysis assay, MTT assay, and live/dead cell assay (n = 3 replicates per assay).

Results

FTIR confirmed the successful integration of azithromycin without altering the hydrogel structure. SEM and AFM revealed uniform, textured coatings with enhanced roughness. Contact angle measurements indicated improved hydrophilicity (58°). Sustained azithromycin release was observed. The azithromycin-loaded coating exhibited superior antibacterial activity, larger inhibition zones, excellent hemocompatibility (0.8 % hemolysis), and 100 % cell viability in fibroblast cultures.

Conclusion

Azithromycin-loaded PVA-fucoidan hydrogel coating on titanium demonstrated promising physicochemical and biological properties, making it a potential candidate for enhancing the performance of titanium-based biomedical implants.

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生物功能阿奇霉素负载聚乙烯醇和岩藻聚糖水凝胶涂层的体外实验研究

钛基植入物因其优异的力学性能和生物相容性而广泛应用于生物医学领域。然而，术后感染和骨融合不足的风险仍然是一个重大挑战。水凝胶具有可调节的特性，为提高钛表面的生物活性提供了一种很有前途的方法。本研究探讨了负载阿奇霉素的pva -岩藻聚糖水凝胶涂层的制备和表征，并对其物理化学和生物学性能进行了评价。目的制备并表征一种负载阿奇霉素的pva岩藻糖聚糖水凝胶，并评价其抗菌、血液相容性和生物相容性。材料与方法制备了25块美国材料试验协会（ASTM） V级纯钛片，并采用射频氩等离子体处理。合成pva -岩藻聚糖水凝胶，并以1% w/w的阿奇霉素负载，电泳沉积于钛表面。利用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、原子力显微镜（AFM）和接触角测量进行表面表征。用紫外-可见分光光度法测定药物体外释放度。检测对变形链球菌和大肠杆菌的抑菌效果（每组3只）。通过溶血试验、MTT试验和活/死细胞试验评估血液相容性和细胞毒性（每个试验n = 3个重复）。结果ftir证实阿奇霉素在不改变水凝胶结构的情况下成功整合。扫描电镜和原子力显微镜显示均匀的、有织构的涂层，粗糙度增强。接触角测量表明亲水性得到改善（58°）。观察到阿奇霉素持续释放。负载阿奇霉素的涂层具有优异的抗菌活性，更大的抑制区，优异的血液相容性（溶血率为0.8%），在成纤维细胞培养中具有100%的细胞存活率。结论负载阿奇霉素的pva岩藻聚糖水凝胶包被钛具有良好的物理化学和生物学性能，是提高钛基生物医学植入物性能的潜在候选材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.