二氯异氰尿酸钠与2-羟基异己酸内源性药物对粪肠球菌抗菌效果的体外比较研究

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-07-31 DOI:10.1016/j.jobcr.2025.07.015

Rasmina K. Nizar, Anju Varughese, M. Remya, V.P. Prabath Singh, Gayathri Usha, Gayathri Presannakumar

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引用次数: 0

摘要

背景：根治根管感染，实现根尖周组织的最佳愈合是根管治疗的主要目标。使用管内药物可以提高根管治疗的抗菌效果。目的比较氢氧化钙（Ca (OH)2）和2%葡萄糖酸氯己定（CHX）配制的二氯异氰尿酸钠（NaDCC）和2-羟基异己酸（HICA）对粪肠球菌（E.faecalis）的抗菌效果。方法对52只单根牙进行根管固定，接种粪肠杆菌菌悬液。14天后，每颗牙齿的根管用Peeso扩眼器磨碎以获得牙本质芯片。孵育24 h后计数菌落形成单位（CFU-1）。将NaDCC、HICA、(Ca (OH)2) + 2% CHX置于牙内培养14 d。采用CFU试验，每隔3个时间间隔观察药物的抗菌效果。结果组间分析采用单因素方差分析进行统计分析，组内分析采用重复测量方差分析并进行Tukey事后检验。与HICA （p值= .000）和Ca (OH)2 + 2% CHX （p值= .000）相比，NaDCC的CFU计数显著降低。24 h后，HICA的CFU计数明显低于Ca (OH)2 + 2% CHX （p值= 0.009）。结论nadcc和HICA对粪肠球菌具有较强的抗菌作用，提示其有可能作为治疗牙髓感染的替代药物。

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Antibacterial efficacy of sodium dichloroisocyanurate and 2-hydroxyisocaproic acid intracanal medicaments on Enterococcus faecalis: A comparative in-vitro study

Background

Optimal healing of periapical tissues by completely eradicating root canal infection is the primary goal of endodontic therapy. The use of intracanal medication enhances the antibacterial efficacy of endodontic treatment.

Aim

Comparative evaluation of antibacterial efficacy of intracanal medicaments such as sodium dichloroisocyanurate (NaDCC) and 2-hydroxyisocaproic acid (HICA) with a formulation of calcium hydroxide (Ca (OH)2) and 2 % chlorhexidine gluconate (CHX) against Enterococcus faecalis(E.faecalis).

Methodology

Fifty-two single-rooted teeth were subjected to endodontic instrumentation and inoculation with E. faecalis microbial suspension. After 14 days, the root canals of each tooth were ground with a Peeso reamer to procure the dentine chips. Colony-forming units (CFU-1) were counted after 24 h of incubation. Intracanal medicaments: NaDCC, HICA, and (Ca (OH)2) plus 2 % CHX were placed in the tooth and incubated for 14 days. The antibacterial effects of the intracanal medicaments were measured using CFU testing at three-time intervals.

Results

Inter-group analysis utilized one-way ANOVA for statistical analysis, and within-group analysis employed repeated-measures ANOVA followed by the Tukey post hoc test. NaDCC exhibited a significantly lower CFU count compared to HICA (p-value = .000) and Ca (OH)₂ + 2 % CHX (p-value = .000). HICA showed a significantly lower CFU count than Ca (OH)₂ + 2 % CHX (p-value = .009) after 24 h.

Conclusion

NaDCC and HICA demonstrate potent antimicrobial efficacy against E. faecalis, suggesting their potential as an alternative intracanal medicament for endodontic infection.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.