Curcumin-infused carboxymethyl cellulose–gelatin gel for oral tissue repair: Preliminary evaluation

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-08-21 DOI:10.1016/j.jobcr.2025.08.023

Senthil Rethinam

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引用次数: 0

Abstract

Background

This study aimed to develop and evaluate a multifunctional oral wound care gel (OWCG) formulated with carboxymethyl cellulose (CMC), gelatin (GEL), and curcumin (CUR) to enhance oral soft tissue regeneration.

Methods

CMC was synthesized through etherification, whereas CUR was extracted using a modified ethanol-based method. OWCG was prepared and crosslinked using 5 % citric acid. Physicochemical characterization including Fourier-transform infrared (FTIR) spectroscopy, high-resolution scanning electron microscopy (HR-SEM), thermogravimetric analysis (TGA), swelling behavior, and in vitro drug release were assessed. Biological evaluations included in vitro anti-inflammatory, antibacterial, and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assays on fibroblast (3T3-L1) cell, and scratch wound healing assays.

Results

OWCG exhibited a maximum swelling capacity of 86 % at neutral pH and 83 % CUR release under alkaline conditions within 24 h. In vitro protein denaturation inhibition was 82.4 %, confirming its anti-inflammatory efficacy. The gel exhibited notable antibacterial activity. MTT assays demonstrated over 90 % cell viability at 48 h, and scratch wound assays showed 90 % wound closure within the same timeframe.

Conclusion

OWCG demonstrated good biocompatibility, antimicrobial efficacy, and wound healing potential, supporting its use in oral wound care.

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姜黄素灌注羧甲基纤维素明胶用于口腔组织修复：初步评价

本研究旨在开发和评估一种由羧甲基纤维素（CMC）、明胶（gel）和姜黄素（CUR）组成的多功能口腔伤口护理凝胶（OWCG），以促进口腔软组织的再生。方法采用醚化法合成scmc，采用改进的乙醇法提取CUR。以5%柠檬酸为交联剂制备OWCG。理化表征包括傅里叶变换红外光谱（FTIR）、高分辨率扫描电镜（HR-SEM）、热重分析（TGA）、溶胀行为和体外药物释放进行了评估。生物学评价包括体外抗炎、抗菌、成纤维细胞（3T3-L1） MTT（3-[4,5-二甲基噻唑-2-基]-2,5二苯基溴化四唑）测定和抓伤愈合测定。结果sowcg在中性pH条件下最大溶胀率为86%，在碱性条件下24h内CUR释放率为83%，体外蛋白变性抑制率为82.4%，证实了其抗炎作用。该凝胶具有明显的抗菌活性。MTT实验显示在48小时内细胞存活率超过90%，而划伤实验显示在相同的时间内伤口愈合率达到90%。结论owcg具有良好的生物相容性、抗菌效果和创面愈合潜力，可用于口腔创面护理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.