Journal of microbiology and biotechnology最新文献_第6页

Latilactobacillus sakei LB-P12 Ameliorates Osteoarthritis by Reducing Cartilage Degradation and Inflammation via Regulation of NF-κB/HIF-2α Pathway. 酒井乳酸杆菌LB-P12通过调节NF-κB/HIF-2α通路减轻软骨退化和炎症，改善骨关节炎。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-02 DOI: 10.4014/jmb.2504.04013

Mikyung Song, Won Jun Kim, Jaeseok Shim, Kyoungsub Song

{"title":"Latilactobacillus sakei LB-P12 Ameliorates Osteoarthritis by Reducing Cartilage Degradation and Inflammation via Regulation of NF-κB/HIF-2α Pathway.","authors":"Mikyung Song, Won Jun Kim, Jaeseok Shim, Kyoungsub Song","doi":"10.4014/jmb.2504.04013","DOIUrl":"10.4014/jmb.2504.04013","url":null,"abstract":"Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage degradation, inflammation, and pain. Recent studies highlight the gut-joint axis, suggesting that gut microbiota influences joint health by modulating systemic inflammation and immune responses. This study investigated the effects of Latilactobacillus sakei LB-P12 on cartilage degradation and joint inflammation in a monosodium iodoacetate induced rat model of OA. OA severity was assessed through histological analysis, weight-bearing and micro-computed tomography (Micro-CT). Serum Interleukin 6 (IL-6) and prostaglandin E2 (PGE2) levels, along with interleukin-1β (Il1b) and matrix metalloproteinase 13 (Mmp13) expression in knee tissue, were measured. Then, the effect of L. sakei LB-P12 on inflammatory responses in interleukin-1β pretreated chondrocytes has also been investigated. The L. sakei LB-P12 improved weight-bearing distribution and reduced cartilage damage based on histological scores. Micro-CT showed increased bone volume fraction and bone mineral density. Treatment reduced serum IL-6 and PGE2 levels and suppressed Il1b and Mmp13 expression in knee tissues. In vitro, L. sakei LB-P12 inhibited lipopolysaccharide induced pro-inflammatory cytokines and nitric oxide production in macrophages. It also downregulated the expression of Epas1, which encodes hypoxia-inducible factor-2α (HIF-2α), and Mmp13 in IL-1β stimulated chondrocytes. L. sakei LB-P12 shows potential as a dietary supplement for alleviating OA-related pain, cartilage degradation, and inflammation by suppressing the nuclear factor-κB (NF-κB)/ HIF-2α pathway.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504013"},"PeriodicalIF":2.5,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Metarhizium koreanum MN031-Mt 46: Nutritional Supplementation to Improve Conidia and Cuticle-Degrading Enzyme Production by Solid-State Fermentation. 朝鲜绿僵菌mn031 - mt46的优化：营养补充提高固态发酵分生孢子和角质层降解酶产量

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-27 DOI: 10.4014/jmb.2412.12079

Suleiman Abba Muazu, Payorm Cobelli, Teerada Wangsomboondee

{"title":"Optimization of Metarhizium koreanum MN031-Mt 46: Nutritional Supplementation to Improve Conidia and Cuticle-Degrading Enzyme Production by Solid-State Fermentation.","authors":"Suleiman Abba Muazu, Payorm Cobelli, Teerada Wangsomboondee","doi":"10.4014/jmb.2412.12079","DOIUrl":"10.4014/jmb.2412.12079","url":null,"abstract":"This study aimed to evaluate five different mixed agricultural wastes as potential substrates for solid-state fermentation (SSF) to produce conidia of Metarhizium koreanum MN031-Mt 46. Single-factor experiments and a Box-Behnken design (BBD) were employed to optimize the fermentation conditions for enhanced conidia yield. Results indicated that a mixed substrate comprising broken rice and rice bran significantly enhanced the optimal production of aerial conidia of MN031-Mt 46. Optimal fermentation conditions established through response surface methodology (RSM) revealed that with the addition of shrimp shell waste to the mixed substrate, conidia production increased to 8.45 × 108 conidia per gram of dry substrate at 26.19°C temperature, 39.76% moisture, and 1.45% of shrimp shell waste after 301.87 h of incubation. Enhanced conidia performance indices were observed, including higher conidia weight, increased water content, and reduced residue post-harvest. The optimized fermentation conditions resulted in enhanced cuticle-degrading enzymatic activities, with maximum activities of 58.78 ± 2.29 U g-1 ds for protease, 126.57 ± 6.47 U g-1 ds for lipase, and 58.32 ± 0.78 U g-1 ds for chitinase. These findings highlight the potential and versatility of mixed SSF using cost-effective agricultural waste for biopesticide and hydrolytic enzyme production, while promoting sustainable waste management and environmental pollution control, aligning with circular economy principles.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412079"},"PeriodicalIF":2.5,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular Calcium Responses to External Calcium Stimuli in Dictyostelium. Dictyostelium细胞内钙对外部钙刺激的反应。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-27 DOI: 10.4014/jmb.2412.12066

Dahyeon Kim, Jiseong Seo, Taeck Joong Jeon

{"title":"Intracellular Calcium Responses to External Calcium Stimuli in Dictyostelium.","authors":"Dahyeon Kim, Jiseong Seo, Taeck Joong Jeon","doi":"10.4014/jmb.2412.12066","DOIUrl":"10.4014/jmb.2412.12066","url":null,"abstract":"Intracellular calcium (Ca2+) plays a vital role in numerous cellular processes, including signal transduction, cell motility, and development. Despite extensive research on intracellular calcium dynamics, the specific mechanisms by which extracellular calcium influences intracellular calcium levels remain unclear. In this study, we generated a Dictyostelium-specific plasmid expressing the calcium biosensor GCaMP3 and visualized real-time intracellular calcium fluctuations in live cells. In response to external calcium, vegetative cells displayed a rapid and transient increase in intracellular calcium levels, peaking within 20 s, whereas aggregation-competent cells showed no such response. Gβ-null cells showed a fast and slight induction at approximately 5 s, with a slower and more efficient, extended high induction at 30 s. Meanwhile, gca/sgc-null cells showed a slightly delayed response, and a significantly lower proportion of iplA-null cells responded to external calcium stimuli compared to wild-type cells. In the presence of W7, a calmodulin antagonist that inhibits calcium release from acidic stores, cells exhibited a marked reduction in the major calcium induction peak at 20 s post-stimulation. Our results suggest that there are at least two pathways to increase the intracellular calcium level in response to external calcium stimuli, and that intracellular acidic calcium stores partially contribute to the second major peak of calcium induction following external stimuli. It appears that G proteins, cGMP, and IplA are involved in calcium homeostasis upon external calcium stimulation, and play an important role in modulating the timing and amplitude of calcium responses.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412066"},"PeriodicalIF":2.5,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neferine Ameliorates Slow-Transmitting Constipation by Inducing PINK1/Parkin-Mediated Mitophagy in Protective Enteric Glial Cells. 通过诱导PINK1/帕金森介导的保护性肠胶质细胞有丝分裂，莲子碱改善慢传性便秘。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-27 DOI: 10.4014/jmb.2412.12022

Taiyu Chen, Xiaodong Jiang, Bo Ma, Yu Zhan, Yong Wen, Lifang Mao, Jun Pang, Xuegui Tang

{"title":"Neferine Ameliorates Slow-Transmitting Constipation by Inducing PINK1/Parkin-Mediated Mitophagy in Protective Enteric Glial Cells.","authors":"Taiyu Chen, Xiaodong Jiang, Bo Ma, Yu Zhan, Yong Wen, Lifang Mao, Jun Pang, Xuegui Tang","doi":"10.4014/jmb.2412.12022","DOIUrl":"10.4014/jmb.2412.12022","url":null,"abstract":"The enteric glial cells (EGCs) are the main components of the enteric nervous system (ENS) and contribute to the development of slow transit constipation (STC). In this study, we aimed to explore the effects of neferine (Nef) on EGCs based on PINK1/Parkin-mediated mitophagy. In vivo, 7 days of loperamide feeding was conducted to model STC rats, which were then treated with 2.5, 5, 10 mg/kg/d Nef, and 2 mg/kg/d mosapride for 14 days. In vitro, a CCK-8 assay was performed to detect EGC viability. EGCs were then stimulated by 400 μM H2O2, transfected with si-PINK1, and treated with Nef or mitochondrial division inhibitor 1 (Mdivi-1). Colon tissue was observed by H&E staining, TEM, ELISA (to quantify SOD, MDA, GDNF, and NGF expression), and immunofluorescence (to count the number of mitochondria). In addition, flow cytometry was used to quantify cell apoptosis, ROS, and mitochondrial membrane potential (MMP). Finally, the p62, PINK1, Parkin, and LC3II/I expression levels were measured by western blotting. Nef was shown to significantly improve STC in rats and reduce mucosal epithelial cell loss, inflammatory cell infiltration, and fibrous proliferation. Moreover, Nef reduced ROS and MDA levels while increasing SOD, GDNF, and NGF. Nef treatment also increased the LC3II/I ratio, as well as p62, PINK1, and Parkin expression, which helped mitigate mitochondrial expansion. However, PINK1 silencing shared the same function as Mdivi-1 in the STC+Nef group, inhibiting EGC viability, oxidative stress, and PINK1/Parkin signaling activation. Additionally, mitophagy was exacerbated by si-PINK1 in the STC+Nef group EGCs. In short, Nef ameliorates STC by inducing PINK1/Parkin-mediated mitophagy in EGCs.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412022"},"PeriodicalIF":2.5,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of Naturally Occurring Diseases by Supernatant from Chlorella Cultures in Pepper. 辣椒小球藻培养上清液对自然病害的治理。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-27 DOI: 10.4014/jmb.2502.02004

Sang-Moo Lee, Hyun Gi Kong, Bongsoo Lee, Yong-Keun Chang, Choong-Min Ryu

{"title":"Management of Naturally Occurring Diseases by Supernatant from Chlorella Cultures in Pepper.","authors":"Sang-Moo Lee, Hyun Gi Kong, Bongsoo Lee, Yong-Keun Chang, Choong-Min Ryu","doi":"10.4014/jmb.2502.02004","DOIUrl":"10.4014/jmb.2502.02004","url":null,"abstract":"The large-scale culture of Chlorella, a genus of microalgae, generates valuable products used to improve human health and produce biofuel. Such commercial applications utilized only the microalgal cells. However, the process produces tons of supernatant waste that require detoxification and disposal. A previous study demonstrated that cell-free supernatants from Chlorella fusca culture primes plant immunity in the model plant Arabidopsis thaliana, suggesting its potential for use as a bioprotectant. The current study investigated the effects of treatment with Chlorella supernatant on crop plants in an agricultural setting. Supernatants from Chlorella sp. strains HS2 and ABC001 were drench-applied to pepper (Capsicum annum L.) seedlings under greenhouse and field conditions. The disease control capacity and growth of plants were evaluated, as well as the diversity of the rhizosphere microbiota. Application of either supernatant reduced the severity of bacterial leaf spot disease caused by Xanthomonas axonopodis pv. vesicatoria and enhanced pepper seedling growth in the greenhouse. Under field conditions, cell-free supernatants of strains HS2 and ABC001 not only reduced the severity of natural-occurring viral and bacterial diseases and insect infestation but also increased fruit yield. Additionally, drenching with Chlorella supernatants improved species diversity in the rhizosphere microbiota. The application of Chlorella supernatant to pepper therefore offered protection against diverse field pathogens and promoted seedling growth and productivity. Our finding provides insight into novel methods of sustainable agriculture utilizing recycled cell-free waste supernatants from the industrial culture of Chlorella.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502004"},"PeriodicalIF":2.5,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Endophytic Bacterium Serratia marcescens B.SB 1.1 associated with Sea Fern (Acrostichum aureum L.) as an Antidiabetic Agent. 海蕨（Acrostichum aureum L.）伴生内生细菌粘质沙雷氏菌bsb 1.1的抗糖尿病作用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-27 DOI: 10.4014/jmb.2412.12031

Tetty Marta Linda, Dinda Putri Maisyaroh, Azizul Berlyansah, Balqis Juanne Tasliyah, Erwina Juliantari, Delita Zul, Bernadeta Leni Fibriarti, Asih Rahayu Ajeng Agesti, Yuli Haryani

{"title":"Efficacy of Endophytic Bacterium Serratia marcescens B.SB 1.1 associated with Sea Fern (Acrostichum aureum L.) as an Antidiabetic Agent.","authors":"Tetty Marta Linda, Dinda Putri Maisyaroh, Azizul Berlyansah, Balqis Juanne Tasliyah, Erwina Juliantari, Delita Zul, Bernadeta Leni Fibriarti, Asih Rahayu Ajeng Agesti, Yuli Haryani","doi":"10.4014/jmb.2412.12031","DOIUrl":"10.4014/jmb.2412.12031","url":null,"abstract":"Diabetes mellitus (DM) is a primary global health concern, often progressing unnoticed until complications arise. Current antidiabetic therapies primarily aim to inhibit the α-amylase enzyme, thereby reducing blood glucose levels. Some medicinal plants are proven to be symbiotic with endophytic bacteria that produce bioactive compounds capable of inhibiting α-amylase activity. This study investigated the potential of endophytic bacteria isolated from the stem of the sea fern (Acrostichum aureum L.) to act as α-amylase inhibitors, using both in vitro and in silico studies. Phytochemical analysis of both the stem extract and cultured bacterial isolates showed the presence of alkaloids, flavonoids, and saponins. Isolate B.SB 1.1 was identified as Serratia marcescens based on 16S rRNA sequencing. The α-amylase inhibition assay demonstrated the strain as showing significant inhibitory activity, with 32.57% inhibition at 2% starch substrate concentration. In silico docking studies using LC-MS data predicted 4-propylbiphenyl and benzoin as compounds with the lowest binding energy to α-amylase, suggesting their potential as effective inhibitors. These findings highlight the efficacy and therapeutic potential of endophytic strain S. marcescens B.SB 1.1 as a novel antidiabetic agent.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412031"},"PeriodicalIF":2.5,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferroptosis-Like Death Induction in Saccharomyces cerevisiae by Gold Nanoparticles. 金纳米颗粒诱导酿酒酵母嗜铁性死亡。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-27 DOI: 10.4014/jmb.2501.01029

Min Seok Kwun, Dong Gun Lee

{"title":"Ferroptosis-Like Death Induction in Saccharomyces cerevisiae by Gold Nanoparticles.","authors":"Min Seok Kwun, Dong Gun Lee","doi":"10.4014/jmb.2501.01029","DOIUrl":"10.4014/jmb.2501.01029","url":null,"abstract":"Ferroptosis, a novel form of regulated cell death (RCD), has emerged as a promising therapeutic strategy for cancer treatment. While gold nanoparticles (AuNPs) are known to induce cell death and ferroptosis in combination with certain antibiotics, the mechanisms underlying ferroptosis in microorganisms remain poorly understood. This study aimed to investigate whether AuNPs induce ferroptosis-like cell death in the eukaryotic microbe Saccharomyces cerevisiae. Our findings revealed that AuNPs significantly reduced cell viability in S. cerevisiae, suggesting their ability to trigger cell death. Ferroptosis-related precursors, including intracellular iron overload and depletion of glutathione (GSH), were observed, leading to the inactivation of glutathione peroxidase (GPx). These changes were associated with the accumulation of reactive oxygen species (ROS) and lipid peroxidation, which amplified oxidative stress within the cells. Elevated ROS levels and lipid peroxidation further resulted in membrane rupture and the formation of 8-hydroxydeoxyguanosine, indicating DNA damage. Mitochondrial dysfunction, a hallmark of ferroptosis, was also evident. AuNP treatment caused mitochondrial membrane potential hyperpolarization and a reduction in mitochondrial membrane density. Unlike previously characterized forms of RCD, ferroptosis-like death in S. cerevisiae did not involve chromatin condensation, DNA fragmentation, or metacaspase activation. Finally, ferroptosis-like characteristics were confirmed using Liperfluo, a lipid ROS-specific probe. In conclusion, this study demonstrated that AuNPs can induce ferroptosis-like cell death in S. cerevisiae. These findings highlight the potential of AuNPs as antifungal agents and contribute to the broader understanding of ferroptosis in eukaryotic microbes.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501029"},"PeriodicalIF":2.5,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Inflammatory Effects of Ajuga decumbens Extract under Blue Light Stimulation. 卧枕草提取物在蓝光刺激下的抗炎作用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-24 DOI: 10.4014/jmb.2502.02002

Tae-Jin Park, Byeong Min Choi, Hyehyun Hong, Jin-Soo Park, Seung-Young Kim

{"title":"Anti-Inflammatory Effects of Ajuga decumbens Extract under Blue Light Stimulation.","authors":"Tae-Jin Park, Byeong Min Choi, Hyehyun Hong, Jin-Soo Park, Seung-Young Kim","doi":"10.4014/jmb.2502.02002","DOIUrl":"10.4014/jmb.2502.02002","url":null,"abstract":"We investigated the potential of Ajuga decumbens Thunb. as an anti-inflammatory agent by utilizing plant resources to develop materials through the application of tissue culture and light-emitting diode (LED) cultivation technologies. To compare the changes between A. decumbens callus extract (ADCB) cultivated under blue monochromatic LED light and ADC (the negative control) cultivated under dark conditions, their morphological characteristics were tested and LC/MS analyses were conducted. ADCB exhibited a greenish hue compared with ADC and contained increased levels of specific compounds. The anti-inflammatory activities of the two samples were evaluated using LPS-stimulated macrophages. None of the samples exhibited cytotoxicity at any tested concentration. However, ADCB demonstrated a greater ability to reduce nitric oxide and key pro-inflammatory cytokines including interleukin-1β, interleukin-6, and tumor necrosis factor-α compared to the control. Furthermore, ADCB effectively suppressed the expression of inducible nitric oxide synthase and cyclooxygenase-2. It inhibited the phosphorylation of mitogen-activated protein kinase family proteins, including extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38, in a concentration-dependent manner. Tissue culture and LED cultivation technologies are significant methods for addressing plant supply challenges and enhancing the content of bioactive compounds, thereby increasing the applicability of plant materials. Moreover, ADCB produced using these technologies exhibited anti-inflammatory activity without causing irritation to human skin at active concentrations, suggesting its potential as a novel anti-inflammatory material.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502002"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cynaropicrin Suppresses Cell Proliferation by Inducing Mitophagy through p38 MAPK-Mediated Mitochondrial ROS Generation in Human Hepatocellular Carcinoma Cells. Cynaropicrin通过p38 mapk介导的线粒体ROS生成诱导线粒体自噬抑制人肝癌细胞增殖

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-24 DOI: 10.4014/jmb.2501.01025

Min Yeong Kim, Hyun Hwangbo, Seon Yeong Ji, Da Hye Kim, Shin-Hyung Park, Su Hyun Hong, Gi Young Kim, EunJin Bang, Yung Hyun Choi

{"title":"Cynaropicrin Suppresses Cell Proliferation by Inducing Mitophagy through p38 MAPK-Mediated Mitochondrial ROS Generation in Human Hepatocellular Carcinoma Cells.","authors":"Min Yeong Kim, Hyun Hwangbo, Seon Yeong Ji, Da Hye Kim, Shin-Hyung Park, Su Hyun Hong, Gi Young Kim, EunJin Bang, Yung Hyun Choi","doi":"10.4014/jmb.2501.01025","DOIUrl":"10.4014/jmb.2501.01025","url":null,"abstract":"Cynaropicrin, a sesquiterpene lactone, has diverse pharmacological activities. However, its anticancer activity against hepatocellular carcinoma (HCC) has not been fully elucidated. Here, we investigated the cytotoxic effects of cynaropicrin and examined its mechanism of action in human HCC cells. The results demonstrated that cynaropicrin significantly induced cytotoxicity and autophagy in HCC cells, but not in immortalized non-cancerous hepatocytes, which was related to the generation of mitochondrial reactive oxygen species (mtROS) and induction of mitochondrial membrane potential loss. Under cynaropicrin treatment, the expression of microtubule-associated protein light chain 3, which is involved in the elongation of the phagophore membrane, was upregulated, whereas the expression of Beclin-1 and p62, which are essential for the formation of autophagosomes, was downregulated. In addition, the expression of mitophagy regulators PTEN-induced kinase 1 (PINK1) and Parkin in the mitochondria increased, suggesting the induction of autophagic flux in the mitochondria. However, N-acetyl-l-cysteine, a ROS scavenger, counteracted cynaropicrin-induced effects. Moreover, cynaropicrin increased the phosphorylation of p38 mitogen-activated protein kinase (MAPK), and the p38 MAPK inhibitor, SB203580, specifically attenuated cynaropicrin-induced cytotoxicity and mtROS production. Importantly, SB203580 reversed cynaropicrin-induced expression of PINK1 and Parkin in the mitochondria. Collectively, our findings demonstrate that cynaropicrin exerts cytotoxic effects against HCC cells by inducing mitochondrial autophagy through the activation of the p38 MAPK-ROS pathway, indicating that cynaropicrin could be a potential therapeutic agent for liver cancer treatment.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501025"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome, Potential Therapeutic Agents: New Players of Obesity Treatment. 微生物群，潜在的治疗剂：肥胖治疗的新参与者。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-24 DOI: 10.4014/jmb.2501.01024

Maqsood Ali, Navid Iqbal, Md Abdur Rakib, Kyung-Ah Lee, Mi-Hwa Lee, Yong-Sik Kim

{"title":"Microbiome, Potential Therapeutic Agents: New Players of Obesity Treatment.","authors":"Maqsood Ali, Navid Iqbal, Md Abdur Rakib, Kyung-Ah Lee, Mi-Hwa Lee, Yong-Sik Kim","doi":"10.4014/jmb.2501.01024","DOIUrl":"10.4014/jmb.2501.01024","url":null,"abstract":"Obesity is a global pandemic, and recent research has established a correlation between the microbiome and obesity, indicating potential treatment possibilities. This review evaluated the potential of the microbiome in treating obesity by targeting anti-adipogenesis and adipose tissue browning mechanisms. The microbiome impacts adipogenesis through lipogenesis and inflammation pathways and influences adipose tissue browning via the secretion of gut hormones and short-chain fatty acids. Understanding these mechanisms could pave the way for interventions targeting the gut microbiome to reduce obesity-related adiposity. While our understanding of the specific microbial species, metabolites, and signaling pathways involved in these processes is still limited, this review highlights the potential of microbiome-based therapies for obesity. Further research focused on identifying key microbial players and their mechanisms of action will be crucial for developing targeted and effective interventions. This will ultimately contribute to a more comprehensive understanding of obesity pathogenesis and facilitate the development of novel therapeutic strategies to combat this global health crisis.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501024"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0