Journal of microbiology and biotechnology最新文献_第6页

Development of a New Isoxsuprine Hydrochloride-Based Hydroxylated Compound with Potent Antioxidant and Anti-Inflammatory Activities. 开发具有强效抗氧化和抗炎活性的新型盐酸异舒普林羟基化合物

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-10-03 DOI: 10.4014/jmb.2405.05031

Chien-Yu Wu, Hsiou-Yu Ding, Tzi-Yuan Wang, Chun-Wei Liu, Jiumn-Yih Wu, Te-Sheng Chang

{"title":"Development of a New Isoxsuprine Hydrochloride-Based Hydroxylated Compound with Potent Antioxidant and Anti-Inflammatory Activities.","authors":"Chien-Yu Wu, Hsiou-Yu Ding, Tzi-Yuan Wang, Chun-Wei Liu, Jiumn-Yih Wu, Te-Sheng Chang","doi":"10.4014/jmb.2405.05031","DOIUrl":"https://doi.org/10.4014/jmb.2405.05031","url":null,"abstract":"The scientific community actively pursuits novel compounds with biological activities. In this context, our study utilized the predicted data mining approach (PDMA), which can efficiently screen out biotransformable precursor candidates to produce new bioactive compounds. The PDMA was applied to Bacillus megaterium tyrosinase (BmTYR) to form new bioactive hydroxyl compounds from isoxsuprine hydrochloride (isoxsuprine). The results show that isoxsuprine could be biotransformed by BmTYR to form a new compound, 3''-hydroxyisoxsuprine. 3''-Hydroxyisoxsuprine exhibited 40- fold and 10-fold higher potent antioxidant and anti-inflammation activities than the precursor, isoxsuprine. The 3''-hydroxyisoxsuprine effectively mitigates the hyperimmune response in RAW 264.7 macrophages by inhibiting the upregulation of pro-inflammatory cytokine (IL-1β and IL-6) and inflammatory enzyme COX-2 gene expression triggered by LPS stimulation. This study illustrates that PDMA is an effective strategy for screening known natural and chemical compounds and for generating new bioactive compounds through biotransformation. Our newly produced compound has potential future applications in pharmacology and biotechnology.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triple-Branch Catalytic Assembly DNAzyme Motivated DNA Tweezer for Sensitive and Reliable mecA Gene Detection in Staphylococcus aureus. 用于灵敏可靠地检测金黄色葡萄球菌中 mecA 基因的三分支催化组装 DNA 酶动机 DNA 镊子

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-10-01 DOI: 10.4014/jmb.2409.09008

Xiaoyang Li, Meiyan Xu, Fangmin Gan, Hui Zhao

{"title":"Triple-Branch Catalytic Assembly DNAzyme Motivated DNA Tweezer for Sensitive and Reliable mecA Gene Detection in Staphylococcus aureus.","authors":"Xiaoyang Li, Meiyan Xu, Fangmin Gan, Hui Zhao","doi":"10.4014/jmb.2409.09008","DOIUrl":"https://doi.org/10.4014/jmb.2409.09008","url":null,"abstract":"Staphylococcus aureus (S. aureus, SA) is one of the most common bacteria in nosocomial infections. Sensitive and efficient analysis of methicillin-resistance of SA is crucial for improving the nursing performance of pneumonia. However, methicillin-resistance analysis with favorable sensitivity and specificity in an enzyme-free manner remains a huge challenge. This paper presents the development of a new fluorescent biosensor for detecting mecA gene using a triple-branch catalytic hairpin assembly (CHA) triggered DNAzyme switch-based DNA tweezer. The SA from the samples are immobilized on the plate's surface using the protein A antibody. The biosensor possesses several key features. Firstly, it utilizes dual signal amplification processes, specifically the triple-branch CHA and DNAzyme controlled DNA tweezer-based signal recycling, to enable mecA detection on the plate. This design enhances the method's sensitivity, resulting in a low limit of detection of 1.5 fM. Secondly, the biosensor does not rely on enzymes for mecA analysis, ensuring a high level of stability during target analysis. Lastly, the method demonstrates a remarkable selectivity by accurately distinguishing target sequences from non-target sequences. The proposed biosensor, which does not require enzymes and has a high level of sensitivity, offers a viable platform for the rapid and simple quantification of mecA in SA.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"1-7"},"PeriodicalIF":2.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kjellmaniella crassifolia Reduces Lipopolysaccharide-Induced Inflammation in Caco-2 Cells and Ameliorates Loperamide-Induced Constipation in Mice. Kjellmaniella crassifolia能减轻脂多糖诱导的Caco-2细胞炎症，并能改善洛哌丁胺诱导的小鼠便秘。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-10-01 DOI: 10.4014/jmb.2407.07036

Kirinde Gedara Isuru Sandanuwan Kirindage, Arachchige Maheshika Kumari Jayasinghe, Mi-Soon Jang, Ka-Jung Lee, Hyun-Jung Yun, Ginnae Ahn, Jae-Young Oh

{"title":"Kjellmaniella crassifolia Reduces Lipopolysaccharide-Induced Inflammation in Caco-2 Cells and Ameliorates Loperamide-Induced Constipation in Mice.","authors":"Kirinde Gedara Isuru Sandanuwan Kirindage, Arachchige Maheshika Kumari Jayasinghe, Mi-Soon Jang, Ka-Jung Lee, Hyun-Jung Yun, Ginnae Ahn, Jae-Young Oh","doi":"10.4014/jmb.2407.07036","DOIUrl":"https://doi.org/10.4014/jmb.2407.07036","url":null,"abstract":"Gastrointestinal disorders are widespread globally, with inflammatory diseases being particularly prominent. This study aimed to investigate the effect of Kjellmaniella crassifolia hot water extract (KCH) on lipopolysaccharide (LPS)-induced inflammation in human intestinal epithelial (Caco-2) cells and loperamide-induced constipation in BALB/c mice. The study's findings revealed that KCH dose-dependently increased the cell viability and reduced the NO production by decreasing the iNOS and COX-2 expression in LPS-stimulated Caco-2 cells. Also, KCH downregulated the mRNA expression of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) by regulating the activation of MAPK and NF-κB signaling pathways in LPS-stimulated Caco-2 cells. In addition, KCH increased the expression levels of tight junction proteins, occludin, ZO-1, and claudin-1 in a dose-dependent manner. Furthermore, in vivo study outcomes demonstrated that KCH improved intestinal transit, increased fecal moisture content, and reduced fecal impaction in constipated mice. KCH decreased the mRNA expression of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α), thereby increasing the expression levels of intestinal tight junction proteins (occludin, ZO-1, and claudin-1) in the small intestine tissues of the experimental mice. These proteins may help regulate intestinal motility and improve stool passage, thus reducing constipation. These findings suggest that KCH could be a promising functional food ingredient for managing intestinal inflammation, inflammation-related disorders, constipation, and the pathophysiology of constipation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"1-11"},"PeriodicalIF":2.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Nitrate and Tryptone to Enhance Growth and Triacylglycerol Accumulation in Phaeodactylum tricornutum. 优化硝酸盐和胰蛋白胨以促进 Phaeodactylum tricornutum 的生长和三酰甘油的积累。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-30 DOI: 10.4014/jmb.2408.08036

Yufang Pan, Zhaowen Hu, Eric Maréchal, Hanhua Hu

{"title":"Optimizing Nitrate and Tryptone to Enhance Growth and Triacylglycerol Accumulation in Phaeodactylum tricornutum.","authors":"Yufang Pan, Zhaowen Hu, Eric Maréchal, Hanhua Hu","doi":"10.4014/jmb.2408.08036","DOIUrl":"https://doi.org/10.4014/jmb.2408.08036","url":null,"abstract":"Phaeodactylum tricornutum, a unicellular diatom, is considered a potential feedstock for the production of biofuel and a promising producer for high-value products eicosapentaenoic acid and fucoxanthin. However, a high-efficient cultivating strategy to achieve commercial production of triacylglycerol (TAG) from the diatom is an urgent demand. In this study, we optimized the content and ratio of nitrate and tryptone in the medium to enhance biomass and TAG accumulation simultaneously. Growth with tryptone as the sole nitrogen gave rise to the lowest cell density but the highest TAG content in P. tricornutum relative to nitrate, nitrite, ammonium or urea cultures. In 500 μM NaNO3 cultures, the growth of P. tricornutum increased with the increasing concentration (from 294 to 7056 μM nitrogen) of supplemented tryptone, however supplementation of high tryptone (≥882 μM nitrogen) decreased the neutral lipid content. Elevating nitrogen concentration from 294 to 882 μM via tryptone addition in 250 μM nitrate culture increased cell densities from day 6 to 10 and neutral lipid content on day 10. In particular, supplementing 588 μM nitrogen of tryptone in the 250 μM nitrate culture gave rise to the highest neutral lipid content on days 8 and 10 (increased by 109% and 62% relative to 500 μM nitrate-sole) with a comparable growth to that in 500 μM nitratesole culture from day 2 to 8. In conclusion, we optimized nitrate/tryptone ratio and found that a suitable tryptone addition to a relatively low nitrate culture was favourable to the biomass and TAG accumulation simultaneously in P. tricornutum.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico Analysis and Biochemical Characterization of Streptomyces PET Hydrolase with Bis(2-Hydroxyethyl) Terephthalate Biodegradation Activity. 具有对苯二甲酸二（2-羟乙基）酯生物降解活性的链霉菌 PET水解酶的硅学分析和生化特征。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-25 DOI: 10.4014/jmb.2404.04030

Gobinda Thapa, So-Ra Han, Prakash Paudel, Min-Su Kim, Young-Soo Hong, Tae-Jin Oh

{"title":"In Silico Analysis and Biochemical Characterization of Streptomyces PET Hydrolase with Bis(2-Hydroxyethyl) Terephthalate Biodegradation Activity.","authors":"Gobinda Thapa, So-Ra Han, Prakash Paudel, Min-Su Kim, Young-Soo Hong, Tae-Jin Oh","doi":"10.4014/jmb.2404.04030","DOIUrl":"10.4014/jmb.2404.04030","url":null,"abstract":"Polyethylene terephthalate (PET), one of the most widely used plastics in the world, causes serious environmental problems. Recently, scientists have been focused on the enzymatic degradation of PET, an environmentally friendly method that offers an attractive approach to the degradation and recycling of PET. In this work, PET hydrolase from Streptomyces sp. W2061 was biochemically characterized, and the biodegradation of PET was performed using the PET model substrate bis (2-hydroxyethyl terephthalate) (BHET). PET hydrolase has an isoelectric point of 5.84, and a molecular mass of about 50.31 kDa. The optimum pH and temperature were 7.0 and 40°C, respectively. LC-MS analysis of the enzymatic products showed that the PET hydrolase successfully degraded a single ester bond of BHET, leading to the formation of MHET. Furthermore, in silico characterization of the PET hydrolase protein sequence and its predicted three-dimensional structure was designed and compared with the well-characterized IsPETase from Ideonella sakaiensis. The structural analysis showed that the (Gly-x1-Ser-x2-Gly) serine hydrolase motif and the catalytic triad (Ser, Asp, and His) were conserved in all sequences. In addition, we integrated molecular dynamics (MD) simulations to analyze the variation in the structural stability of the PET hydrolase in the absence and presence of BHET. These simulations showed the formation of a stable complex between the PET hydrolase and BHET. To the best of our knowledge, this is the first study on Streptomyces sp. W2061 to investigate the BHET degradation activity of PET hydrolase, which has potential application in the biodegradation of plastics in the environment.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1836-1847"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Synbiotics as a Therapy to Protect Mental Health in Alzheimer's Disease. 利用合成益生菌保护阿尔茨海默氏症患者的心理健康。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-06-21 DOI: 10.4014/jmb.2403.03021

Anh Pham Thi Ngoc, Adil Zahoor, Dong Gyun Kim, Seung Hwan Yang

{"title":"Using Synbiotics as a Therapy to Protect Mental Health in Alzheimer's Disease.","authors":"Anh Pham Thi Ngoc, Adil Zahoor, Dong Gyun Kim, Seung Hwan Yang","doi":"10.4014/jmb.2403.03021","DOIUrl":"10.4014/jmb.2403.03021","url":null,"abstract":"Alzheimer's disease (AD) is a progressive neurological disorder that represents a major cause of dementia worldwide. Its pathogenesis involves multiple pathways, including the amyloid cascade, tau protein, oxidative stress, and metal ion dysregulation. Recent studies have suggested a critical link between changes in gut microbial diversity and the disruption of the gut-brain axis in AD. Previous studies primarily explored the potential benefits of probiotics and prebiotics in managing AD. However, studies have yet to fully describe a novel promising approach involving the use of synbiotics, which include a combination of active probiotics and new-generation prebiotics. Synbiotics show potential for mitigating the onset and progression of AD, thereby offering a holistic approach to address the multifaceted nature of AD. This review article primarily aims to gain further insights into the mechanisms of AD, specifically the intricate interaction between gut bacteria and the brain via the gut-brain axis. By understanding this relationship, we can identify potential targets for intervention and therapeutic strategies to combat AD effectively. This review also discusses substantial evidence supporting the role of synbiotics as a promising AD treatment that surpasses traditional probiotic or prebiotic interventions. We find that synbiotics may be used not only to address cognitive decline but also to reduce AD-related psychological burden, thus enhancing the overall quality of life of patients with AD.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1739-1747"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of High-Temperature Feeds on Gut Microbiota and MAFLD. 高温饲料对肠道微生物群和 MAFLD 的影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-31 DOI: 10.4014/jmb.2405.05023

Lijun Xue, Kaimin Li, Yanfei Jia, Dongxue Yao, Xuexing Guo, Shuhong Zhang

引用次数: 0

Enhancing Protein Content in Wild-Type Saccharomyces cerevisiae via Random Mutagenesis and Optimized Fermentation Conditions. 通过随机突变和优化发酵条件提高野生型酿酒酵母的蛋白质含量

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-31 DOI: 10.4014/jmb.2405.05027

Sang-Hun Do, Tae-Gi Lee, Sun-Ki Kim

{"title":"Enhancing Protein Content in Wild-Type Saccharomyces cerevisiae via Random Mutagenesis and Optimized Fermentation Conditions.","authors":"Sang-Hun Do, Tae-Gi Lee, Sun-Ki Kim","doi":"10.4014/jmb.2405.05027","DOIUrl":"10.4014/jmb.2405.05027","url":null,"abstract":"Single-cell protein (SCP) derived from microorganisms is widely recognized as a viable alternative protein source for the future. Nevertheless, the commercialization of yeast-based SCP is hampered by its relatively low protein content. Therefore, this study aimed to enhance the protein content of Saccharomyces cerevisiae via random mutagenesis. To achieve this, S. cerevisiae KCCM 51811, which exhibited the highest protein concentration among 20 edible S. cerevisiae strains, was selected as a chassis strain. Subsequently, a KCCM 51811 mutant library was constructed (through UV irradiation) and screened to isolate mutants exhibiting high protein content and/or concentration. Among the 174 mutant strains studied, the #126 mutant exhibited a remarkable 43% and 36% higher protein content and concentration, respectively, compared to the parental strain. Finally, the #126 mutant was cultured in a fed-batch system using molasses and corn-steep liquor, resulting in a protein concentration of 21.6 g/l in 100 h, which was 18% higher than that produced by the parental strain. These findings underscore the potential of our approach for the cost-effective production of food-grade SCP.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1912-1918"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antidepressant Effect and Mechanism of Picea mariana Essential Oil on Reserpine-Induced Depression Model Mice. 红豆杉精油对瑞舍平诱导的抑郁模型小鼠的抗抑郁作用及其机制

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-18 DOI: 10.4014/jmb.2405.05013

Ying Wang, Guofeng Shi, Yixi Zeng, Juting Li, Yongyu Wu, Jiahui Zheng, Anjing Xu, Yanqing Ma, Lanyue Zhang, Hui Li

{"title":"Antidepressant Effect and Mechanism of Picea mariana Essential Oil on Reserpine-Induced Depression Model Mice.","authors":"Ying Wang, Guofeng Shi, Yixi Zeng, Juting Li, Yongyu Wu, Jiahui Zheng, Anjing Xu, Yanqing Ma, Lanyue Zhang, Hui Li","doi":"10.4014/jmb.2405.05013","DOIUrl":"10.4014/jmb.2405.05013","url":null,"abstract":"The disturbance of brain biochemical substances serves as a primary cause and aggravating factor of depression. This study aimed to investigate the principal components of Picea mariana and its effect on reserpine-induced depression mice,w ith its relationship with brain central transmitters and related proteins. The main constituents of P. mariana essential oil (PMEO) were analyzed by GC-MS spectrometry. The quiescent time in the tail suspension test (TST) and forced swim test (FST), along with the weight change of the mice was detected. The number of normal neurons was quantified through Nissl staining. Immunohistochemistry was employed to determine the levels of 5HT-1A and 5HT-2A in the brain. Western blotting was utilized to detect 5HT-2A, CRF and TrkB protein levels. RT-qPCR was used to detect the mRNA levels of 5HT-1A, 5HT-2A, TrkB, CRF, and BDNF. The main active ingredients of PMEOs were (-) -bornyl acetate (44.95%), γ-Terpinene (14.17%), and β-Pinene (10.12%). PMEOs effectively improved the retardation and weight loss due to anorexia in depression-like mice. This improvement was associated with an increase in the number of normal neurons. After administering different doses of PMEOs, the levels of 5HT-1A, 5HT-2A, CRF, and TrkB were found to be increased in brain tissue. RT-qPCR revealed that the mRNA levels of CRF, 5HT-1A, and 5HT-2A were generally upregulated, whereas TrkB and BDNF were downregulated. PMEO can effectively alleviate depression induced by reserpine, which may be attributed to its regulation of 5HT-1A, 5HT-2A, CRF and TrkB protein expression, thus reducing brain nerve injury.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1778-1788"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutagenicity and Genotoxicity Assessment of Leuconostoc lactis DMLL10 Isolated from Kimchi. 从泡菜中分离出的乳酸白球菌 DMLL10 的突变性和遗传毒性评估

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-08-05 DOI: 10.4014/jmb.2405.05025

Heejung Park, Seoyeon Lee, Sojeong Heo, Do-Won Jeong

{"title":"Mutagenicity and Genotoxicity Assessment of Leuconostoc lactis DMLL10 Isolated from Kimchi.","authors":"Heejung Park, Seoyeon Lee, Sojeong Heo, Do-Won Jeong","doi":"10.4014/jmb.2405.05025","DOIUrl":"10.4014/jmb.2405.05025","url":null,"abstract":"Leuconostoc lactis DMLL10 is a microorganism specific to kimchi fermentation. In this study, we sought to evaluate the toxicity of this strain, which was newly isolated from kimchi, to determine its safety as a food ingredient. Bacterial reverse mutation assay, chromosomal aberration assay, and mammalian cell in vitro micronucleus assay were performed to assess the genetic toxicity of Leu. lactis DMLL10. The strain did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537, or Escherichia coli WP2uvrA, with or without metabolic activation of S9 mixture. The oral administration of Leu. lactis DMLL10 also did not significantly increase the number of micronucleated polychromatic erythrocytes, or the mean ratio of polychromatic to total erythrocytes. Additionally, Leu. lactis DMLL10 did not cause a significant chromosomal aberration in CHU/IL cells in the presence or absence of S9 activation. Therefore, Leu. lactis DMLL10 can be suggested as a functional food ingredient with reliability and safety.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1803-1809"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0