Journal of microbiology and biotechnology最新文献_第6页

Isolation and Purification of Antibacterial Compound from Kombucha of SCOBY. SCOBY康普茶中抗菌化合物的分离纯化。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-12 DOI: 10.4014/jmb.2504.04012

Younhee Nam, Junghoon Lee, Soo Rin Kim, Jong Nam Kim

{"title":"Isolation and Purification of Antibacterial Compound from Kombucha of SCOBY.","authors":"Younhee Nam, Junghoon Lee, Soo Rin Kim, Jong Nam Kim","doi":"10.4014/jmb.2504.04012","DOIUrl":"10.4014/jmb.2504.04012","url":null,"abstract":"The misuse of antibiotics has contributed to the widespread emergence of antimicrobial resistance (AMR), emphasizing the need for alternative antimicrobial agents. Kombucha, a fermented beverage containing a symbiotic culture of bacteria and yeast (SCOBY), has gained attention for its antibacterial activity and potential health benefits. This study investigated the antibacterial properties of kombucha and SCOBY, isolating and characterizing the active compounds responsible for these effects. Both kombucha broth and dried SCOBY effectively inhibited Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella Typhimurium, with dried SCOBY demonstrating stronger activity. Instrumental analyses identified 5-hydroxymethylfurfural (HMF) as the primary antibacterial compound in the SCOBY extracts. HMF significantly inhibited L. monocytogenes and S. aureus, with its antibacterial inhibition surpassing that of chloramphenicol in these two bacterial species. Previous studies have shown that, in addition to its antibacterial effects, HMF has potential applications in the production of polymers and pharmaceuticals, and as a fuel additive, suggesting its potential in the chemical and biofuel industries. This study highlights the antibacterial activity of HMF and underscores the need for further research to evaluate its safety and applicability in various fields.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504012"},"PeriodicalIF":3.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reviving Furosemide as a Metallo-β-Lactamase Inhibitor against MDR Acinetobacter baumannii. 恢复速尿作为抗耐多药鲍曼不动杆菌的金属β-内酰胺酶抑制剂。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-07 DOI: 10.4014/jmb.2506.06023

Hanan Al-Lawati, Fatma Al-Raisi, Mahmoud A Elfaky, Mahmoud Saad Abdel-Halim, Hisham A Abbas, Basem Mansour, Wael A H Hegazy, Noura M Seleem

{"title":"Reviving Furosemide as a Metallo-β-Lactamase Inhibitor against MDR Acinetobacter baumannii.","authors":"Hanan Al-Lawati, Fatma Al-Raisi, Mahmoud A Elfaky, Mahmoud Saad Abdel-Halim, Hisham A Abbas, Basem Mansour, Wael A H Hegazy, Noura M Seleem","doi":"10.4014/jmb.2506.06023","DOIUrl":"10.4014/jmb.2506.06023","url":null,"abstract":"Carbapenems are considered the last line of antibiotic defense against multidrug-resistant (MDR) Acinetobacter baumannii, a bacterium that can secrete carbapenemases such as metallo-β-lactamases (MBLs) to degrade carbapenems. It is thus critical to develop strategies to combat carbapenem resistance, and one of these strategies is to discover MBL inhibitors. In the current study, we evaluated the possible anti-β-lactamase of the approved safe drug furosemide. Furosemide is a loop diuretic with antihypertensive effects. A clinical MDR and carbapenem-resistant A. baumannii isolate was used. Furosemide at 1 mg/ml potentiated meropenem in the combined disk method and interfered with the hydrolytic activities of MBL. Furthermore, furosemide synergized meropenem and decreased its minimum inhibitory concentration (MIC). Furosemide could also reduce the expression of the MBL genes blaNDM and blaVIM. In silico study showed the binding ability of furosemide with the active sites of New Delhi and VIM MBL enzymes, as well as its chelating interaction with zinc ions. Furosemide is a promising MBL inhibitor that can be used in combination with meropenem to treat infections caused by MBL-producing A. baumannii.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506023"},"PeriodicalIF":3.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory and Antioxidant Activities of Saccharina japonica Extracts Processed by Subcritical Water Extraction and Enterococcus faecalis Fermentation. 亚临界水浸提与粪肠球菌发酵对槐花提取物抑菌及抗氧化活性的影响

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-07 DOI: 10.4014/jmb.2504.04045

Yong Zhao, Chae Hun Ra

{"title":"Inhibitory and Antioxidant Activities of Saccharina japonica Extracts Processed by Subcritical Water Extraction and Enterococcus faecalis Fermentation.","authors":"Yong Zhao, Chae Hun Ra","doi":"10.4014/jmb.2504.04045","DOIUrl":"10.4014/jmb.2504.04045","url":null,"abstract":"Marine algae, notably Saccharina japonica, are valuable sources of essential nutrients, including carbohydrates, vitamins, and minerals, as well as bioactive compounds, such as phenols and pigments. In this study, subcritical water extraction of S. japonica, a brown seaweed, was conducted using a 10% (w/v) seaweed slurry at a temperature of 140°C for 10 min. The concentrations of mannitol, laminarin, and total phenolic content were determined to be 18.93 g/l, 10.79 g/l, and 1.94 mg GAE/g, respectively. The S. japonica fermented using Enterococcus faecalis (SFL) resulted in a maximum biomass production of 4.43 g dcw/l and exhibited an inhibitory activity of 78.69% against pancreatic lipase. The S. japonica fermented using E. faecalis (SFE) extracts exhibited α-glucosidase inhibitory and antioxidant activities of 59.23% and 65.60%-66.57%, respectively, at a concentration of 3.0 mg/ml. These results facilitate the development of functional seaweed food formulations derived from microbial fermentation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504045"},"PeriodicalIF":3.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotransformation of Sophorabioside in the Fruits and Small Branches of Sophora japonica into Sophoricoside Using α-L-Rhamnosidase from Chloroflexus aurantiacus. 利用金花莲α- l -鼠李糖苷酶将苦参果实和小枝中的苦参苷转化为苦参苷。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-07 DOI: 10.4014/jmb.2505.05034

Su-Hwan Kang, Yeong-Su Kim, Hwan Lee, Kyung-Chul Shin

{"title":"Biotransformation of Sophorabioside in the Fruits and Small Branches of Sophora japonica into Sophoricoside Using α-L-Rhamnosidase from Chloroflexus aurantiacus.","authors":"Su-Hwan Kang, Yeong-Su Kim, Hwan Lee, Kyung-Chul Shin","doi":"10.4014/jmb.2505.05034","DOIUrl":"10.4014/jmb.2505.05034","url":null,"abstract":"Sophora japonica L., a medicinal herb used in East Asia, contains bioactive flavonoids such as sophoricoside and sophorabioside. Sophoricoside, a more bioavailable and therapeutically potent form, is known for its pharmacological properties, including anti-inflammatory, -cancer, and -osteoporotic effects. However, conversion of sophorabioside into sophoricoside has not been extensively studied. In this study, we aimed for the enzymatic conversion of sophorabioside, abundant in the S. japonica fruits and small branches, into sophoricoside using α-L-rhamnosidase from Chloroflexus aurantiacus. Optimal reaction conditions for this biotransformation were established, with maximal enzymatic activity observed at pH 6.0 and 55°C. Substrate specificity analysis revealed high selectivity of the enzyme for neohesperidose-containing flavonoids, including sophorabioside. Sophoricoside was efficiently produced from S. japonica fruit and branch extracts, enhanced by 2.5% methanol. The biotransformed extracts demonstrated significant lipoxygenase inhibitory activity, suggesting enhanced anti-inflammatory potential, highlighting the potential of S. japonica-derived sophoricoside-enriched products as functional food supplements with improved therapeutic properties.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505034"},"PeriodicalIF":3.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polystyrene Accelerates Aging Related-Gut Microbiome Dysbiosis and -Metabolites in Old-Aged Mouse. 聚苯乙烯加速衰老相关的老年小鼠肠道微生物群失调和代谢物。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-07 DOI: 10.4014/jmb.2504.04016

Hyun Hwangbo, Eun-Ju Kim, Gi-Young Kim, Sun-Young Hwang, Mee-Hyun Lee, Yung Hyun Choi

{"title":"Polystyrene Accelerates Aging Related-Gut Microbiome Dysbiosis and -Metabolites in Old-Aged Mouse.","authors":"Hyun Hwangbo, Eun-Ju Kim, Gi-Young Kim, Sun-Young Hwang, Mee-Hyun Lee, Yung Hyun Choi","doi":"10.4014/jmb.2504.04016","DOIUrl":"10.4014/jmb.2504.04016","url":null,"abstract":"Microplastics, particularly polystyrene (PS), are ubiquitous environmental contaminants and concerns about their potential detrimental effects on human health are increasing. Emerging evidence suggests that microplastics may disrupt the gut microbiota, a critical ecosystem involved in regulating host metabolism, immunity, and aging processes. However, the specific effects of PS on the gut microbiota composition and its potential role in modulating aging are yet to be fully elucidated. In this study, we aimed to investigate the effects of PS exposure on gut microbiota dysbiosis and its potential role in the acceleration of aging. Gut microbiota composition was assessed using 16S rDNA sequencing, while fecal metabolites were analyzed using gas chromatography-mass spectrometry. Exposure to PS resulted in a significant reduction in the abundance of beneficial microbiota, including Blautia. In contrast, there was an increase in the relative abundance of potentially harmful taxa, such as Lachnospiraceae UCG-001, and Candidatus Arthromitus. Metabolomic analysis revealed elevated levels of several metabolites associated with stress responses and altered host metabolism, including alanine, serine, tryptophan, 5-aminovaleric acid, thymine, threonine, methionine, and benzoic acid. These findings demonstrate that PS exposure in aged mice exacerbated gut microbiome dysbiosis and altered key metabolic markers associated with aging, suggesting an increased vulnerability to age-related diseases as a consequence of microplastic exposure.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504016"},"PeriodicalIF":3.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut Microbiome-Based Strategies for the Control of Carbapenem-Resistant Enterobacteriaceae. 基于肠道微生物组的碳青霉烯耐药肠杆菌科控制策略。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-07 DOI: 10.4014/jmb.2406.06017

Imchang Lee, Bong-Soo Kim, Ki Tae Suk, Seung Soon Lee

{"title":"Gut Microbiome-Based Strategies for the Control of Carbapenem-Resistant Enterobacteriaceae.","authors":"Imchang Lee, Bong-Soo Kim, Ki Tae Suk, Seung Soon Lee","doi":"10.4014/jmb.2406.06017","DOIUrl":"10.4014/jmb.2406.06017","url":null,"abstract":"Carbapenem-resistant Enterobacteriaceae (CRE) represent a critical antimicrobial resistance threat due to their resistance to last-resort antibiotics and high transmission potential. While conventional strategies-such as infection control, antimicrobial stewardship, and novel antibiotic development-remain essential, growing attention has shifted toward the gut microbiome, which plays a central role in mediating colonization resistance against CRE. Disruption of the intestinal microbiota-primarily driven by antibiotic exposure and further exacerbated by non-antibiotic drugs such as proton pump inhibitors-reduces microbial diversity and impairs functional integrity, facilitating CRE acquisition, prolonged carriage, and horizontal transmission. In response, microbiome-based strategies-including microbiome disruption indices (MDIs), fecal microbiota transplantation (FMT), and rationally designed symbiotic microbial consortia-are being explored as novel approaches for CRE prevention and decolonization. Mechanistic studies have shown that colonization resistance is mediated by both direct mechanisms (e.g., nutrient competition, short-chain fatty acid production) and indirect mechanisms (e.g., immune modulation via IL-36 signaling). Advances in metagenomics, metabolomics, and culturomics have enabled high-resolution profiling of gut microbial communities and their functional roles. Emerging preclinical and clinical evidence supports the potential of microbiome-informed interventions to predict infection risk, enhance antimicrobial stewardship, and guide the development of next-generation probiotics targeting CRE. Longitudinal studies continue to evaluate the efficacy of FMT and synthetic microbial consortia in eradicating intestinal CRE colonization. Collectively, these insights underscore the promise of gut microbiome science as a complementary and innovative strategy for CRE control in the post-antibiotic era.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2406017"},"PeriodicalIF":3.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, Expression, and Characterization of GDSL-Type Lipolytic Enzyme Genes from Epidermidibacterium keratini EPI-7 Isolated from Human Skin. 人皮肤角化表皮杆菌EPI-7 gdsl型脂溶酶基因的克隆、表达和特性研究

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-06 DOI: 10.4014/jmb.2504.04022

Seok-Yun Jeong, Seok Kyun Yun, Suhyeon Cho, Seyeol Baek, Hee-Jae Shin, Seokmuk Park, Sugyeong Jeong, Gayoung Kim, Seunghyun Kang, Seunghee Bae

{"title":"Cloning, Expression, and Characterization of GDSL-Type Lipolytic Enzyme Genes from Epidermidibacterium keratini EPI-7 Isolated from Human Skin.","authors":"Seok-Yun Jeong, Seok Kyun Yun, Suhyeon Cho, Seyeol Baek, Hee-Jae Shin, Seokmuk Park, Sugyeong Jeong, Gayoung Kim, Seunghyun Kang, Seunghee Bae","doi":"10.4014/jmb.2504.04022","DOIUrl":"10.4014/jmb.2504.04022","url":null,"abstract":"This study investigated seven putative lipolytic enzymes (EstEk01-07) from the skin microbiome bacterium Epidermidibacterium keratini EPI-7, focusing on their properties relevant to industrial applications. Sequence analysis revealed conserved GDSL motifs and four conserved blocks, characteristic of the GDSL/SGNH superfamily, with predicted α/β/α folds consistent with these enzymes. Significant variations in the number of α-helices and β-sheets among the EstEk enzymes suggested diverse substrate specificities and catalytic efficiencies. The enzymes exhibited a strong preference for short-chain fatty acids (C2-C4), classifying them as carboxylesterases, a novel finding within the skin microbiome. Optimal enzyme activity was observed at alkaline pH (8.0-9.0) and thermophilic condition (50-60°C), with substantial thermostability retained after heating at 50°C for three hours. Metal ion analysis revealed a significant stimulatory effect of Ca2+ and Fe3+, while other transition metals were inhibitory. The enzymes were stable in a range of non-ionic detergents, but sensitive to SDS. Moreover, they exhibited notable tolerance to various organic solvents, particularly methanol and isopropanol, suggesting potential applications in cosmetics and pharmaceutical industries. This study identifies a novel library of thermostable, alkaline carboxylesterases from the skin microbiome, highlighting their potential for industrial biocatalysis and further investigation into their role in skin lipid metabolism.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504022"},"PeriodicalIF":3.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of Curcuma longa L. on Corticosterone-Induced Neurotoxicity and Anti-Depression-Like Behavior: Involvement of NMDA and 5-HT_7A Receptors. 姜黄对皮质酮诱导的神经毒性和抗抑郁样行为的保护作用：NMDA和5-HT7A受体的参与

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-05 DOI: 10.4014/jmb.2506.06005

Kyo-Nyeo Oh, Dool-Ri Oh, Youngbae Kim, Sunoh Kim

{"title":"Protective Effects of Curcuma longa L. on Corticosterone-Induced Neurotoxicity and Anti-Depression-Like Behavior: Involvement of NMDA and 5-HT7A Receptors.","authors":"Kyo-Nyeo Oh, Dool-Ri Oh, Youngbae Kim, Sunoh Kim","doi":"10.4014/jmb.2506.06005","DOIUrl":"10.4014/jmb.2506.06005","url":null,"abstract":"Curcuma longa L. is a traditional medicinal plant known for its therapeutic potential in mental disorders, including depression. Previous studies have reported its antidepressant-like effects in animal models, primarily attributed to curcumin, its major bioactive compound. However, the underlying mechanisms of these effects remain poorly understood. In this study, we investigated the neuroprotective and antidepressant-like effects of C. longa through the N-methyl-D-aspartate (NMDA) receptor-linked corticosterone (CORT)-induced neurotoxicity pathway in primary cultured rat cortical neurons. Among various extraction methods tested, the 80% ethanol extract of C. longa (CL-80) exhibited the highest neuroprotective activity and the highest concentration of curcuminoids, including curcumin. Further fractionation of the CL-80 by polarity revealed that the n-butanol fraction (n-BuOH Fr.) demonstrated the most potent neuroprotective effect, with curcumin identified as the primary active constituent. Additionally, serotonin (5-hydroxytryptamine; 5-HT) receptor screening indicated that both the CL-80 and curcumin selectively modulate the 5-HT7A receptor. In a restraint stress-induced depression mouse model, CL-80 and curcumin administration significantly mitigated stress-induced behavioral changes, including the prolongation of immobility time and reduction in climbing time during the forced swim test (FST). These findings suggest that C. longa exerts its antidepressant-like effects via modulation of NMDA and 5-HT7A receptor-mediated pathways, highlighting its potential for development as a novel antidepressant agent.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506005"},"PeriodicalIF":3.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of N-Acetylcysteine (NAC) on oxLDL-Induced Endothelial Dysfunction. n -乙酰半胱氨酸（NAC）对氧化低密度脂蛋白诱导内皮功能障碍的保护作用。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-05 DOI: 10.4014/jmb.2504.04039

Chathuri K Marasinghe, Indyaswan Tegar Suryaningtyas, Won-Kyo Jung, Jae-Young Je

{"title":"Protective Effect of N-Acetylcysteine (NAC) on oxLDL-Induced Endothelial Dysfunction.","authors":"Chathuri K Marasinghe, Indyaswan Tegar Suryaningtyas, Won-Kyo Jung, Jae-Young Je","doi":"10.4014/jmb.2504.04039","DOIUrl":"10.4014/jmb.2504.04039","url":null,"abstract":"N-acetylcysteine (NAC), a well-known antioxidant and glutathione precursor, has been extensively studied for its free radical-scavenging properties, anti-inflammatory effects, and ability to enhance cellular redox balance. NAC has also been shown to mitigate oxidative damage in various disease models, yet its role in endothelial dysfunction remains underexplored. In this study, we evaluated the ability of NAC to counteract oxLDL-induced endothelial dysfunction in human umbilical vein endothelial cells (HUVECs). NAC treatment significantly reduced ROS levels, lipid peroxidation, and apoptotic markers while restoring mitochondrial membrane potential (MMP) and NO bioavailability. Additionally, NAC regulated the expression of eNOS, LOX-1, ICAM-1, and VCAM-1, demonstrating its role in reducing endothelial inflammation and improving vascular homeostasis. Furthermore, NAC prevented excessive cholesterol accumulation, suggesting its potential to regulate lipid metabolism in endothelial cells. These findings highlight the therapeutic potential of NAC in protecting against oxLDL-induced endothelial dysfunction and preventing vascular complications associated with cardiovascular diseases.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504039"},"PeriodicalIF":3.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative Effect of Heat-Killed Lactiplantibacillus plantarum Q1 (LPQ1) on Lipid Accumulation: Integration of Network Pharmacology with In Vitro and In Vivo Experiments. 热杀植物乳杆菌Q1 （LPQ1）对脂质积累的改善作用：网络药理学与体内外实验的结合

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-29 DOI: 10.4014/jmb.2503.03038

Young-Jin Choi, Nishala Erandi Wedamulla, Seok-Hee Kim, Qun Zhang, Duseong Kim, Ji-Won Song, Seunghun Lee, Haeyoung Lee, Jeung Hee An, Ji Young Hwang, Eun-Kyung Kim

{"title":"Ameliorative Effect of Heat-Killed Lactiplantibacillus plantarum Q1 (LPQ1) on Lipid Accumulation: Integration of Network Pharmacology with In Vitro and In Vivo Experiments.","authors":"Young-Jin Choi, Nishala Erandi Wedamulla, Seok-Hee Kim, Qun Zhang, Duseong Kim, Ji-Won Song, Seunghun Lee, Haeyoung Lee, Jeung Hee An, Ji Young Hwang, Eun-Kyung Kim","doi":"10.4014/jmb.2503.03038","DOIUrl":"10.4014/jmb.2503.03038","url":null,"abstract":"Lipid metabolism disorders are expanding at an alarming rate worldwide pressuring the entire healthcare system. Therefore, exploring effective therapeutic treatments for lipid metabolism disorders is a hot spot in research. The therapeutic effects of paraprobiotics on obesity are still not fully explored through the integration of network pharmacology analysis with in vitro and in vivo experiments to confirm their beneficial effect. Therefore, the current study aimed to investigate the ameliorative effect of heat-killed Lactiplantibacillus plantarum Q1 (LPQ1) on lipid accumulation through the integration of network pharmacology analysis with in vitro and in vivo experiments. Findings of network pharmacology analysis conform with in vitro and in vivo experiments, revealing that the modulating effect of LPQ1 on lipid metabolism was effective through the regulation of PPAR-γ and TNF-α expression. Therefore, LPQ1 can be promoted as an effective therapeutic agent against lipid metabolism disorders owing to its modulatory effect on hepatic lipid metabolism and inflammation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503038"},"PeriodicalIF":3.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12325000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0