Isolation and Purification of Antibacterial Compound from Kombucha of SCOBY.

IF 3.1 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Younhee Nam, Junghoon Lee, Soo Rin Kim, Jong Nam Kim
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Abstract

The misuse of antibiotics has contributed to the widespread emergence of antimicrobial resistance (AMR), emphasizing the need for alternative antimicrobial agents. Kombucha, a fermented beverage containing a symbiotic culture of bacteria and yeast (SCOBY), has gained attention for its antibacterial activity and potential health benefits. This study investigated the antibacterial properties of kombucha and SCOBY, isolating and characterizing the active compounds responsible for these effects. Both kombucha broth and dried SCOBY effectively inhibited Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella Typhimurium, with dried SCOBY demonstrating stronger activity. Instrumental analyses identified 5-hydroxymethylfurfural (HMF) as the primary antibacterial compound in the SCOBY extracts. HMF significantly inhibited L. monocytogenes and S. aureus, with its antibacterial inhibition surpassing that of chloramphenicol in these two bacterial species. Previous studies have shown that, in addition to its antibacterial effects, HMF has potential applications in the production of polymers and pharmaceuticals, and as a fuel additive, suggesting its potential in the chemical and biofuel industries. This study highlights the antibacterial activity of HMF and underscores the need for further research to evaluate its safety and applicability in various fields.

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SCOBY康普茶中抗菌化合物的分离纯化。
抗生素的滥用导致了抗菌素耐药性(AMR)的广泛出现,强调需要替代抗菌素药物。康普茶是一种含有细菌和酵母共生培养物(SCOBY)的发酵饮料,因其抗菌活性和潜在的健康益处而受到关注。本研究考察了康普茶和SCOBY的抗菌特性,分离并表征了这些作用的活性化合物。红茶菌汤和干SCOBY均能有效抑制大肠杆菌、单核增生李斯特菌、金黄色葡萄球菌和鼠伤寒沙门菌,其中干SCOBY的抑制作用更强。仪器分析表明,5-羟甲基糠醛(HMF)是SCOBY提取物中的主要抗菌化合物。HMF对单核增生乳杆菌和金黄色葡萄球菌的抑菌作用明显优于氯霉素。先前的研究表明,除了抗菌作用外,HMF在聚合物和药品的生产以及作为燃料添加剂方面具有潜在的应用,这表明它在化学和生物燃料工业中具有潜力。本研究强调了HMF的抗菌活性,并强调需要进一步研究以评估其在各个领域的安全性和适用性。
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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