Journal of microbiology and biotechnology最新文献

Aspirin Combined with Antifungal Drugs Suppresses Candida albicans Biofilm by Activating Autophagy. 阿司匹林与抗真菌药物联用可通过激活自噬抑制白色念珠菌生物膜

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-27 DOI: 10.4014/jmb.2411.11060

Yun Huang, Haochen Miao, Ying Lv, Yang Wang, Shenjun Yu, Xin Wei

{"title":"Aspirin Combined with Antifungal Drugs Suppresses Candida albicans Biofilm by Activating Autophagy.","authors":"Yun Huang, Haochen Miao, Ying Lv, Yang Wang, Shenjun Yu, Xin Wei","doi":"10.4014/jmb.2411.11060","DOIUrl":"https://doi.org/10.4014/jmb.2411.11060","url":null,"abstract":"Aspirin (ASA) induces autophagic death of human tumor cells and autophagy changes the susceptibility of Candida albicans biofilm to antifungal agents. This study investigates whether ASA suppresses C. albicans biofilm by autophagy regulation and its combination effect with antifungals. Biofilm sensitivity to ASA alone and in combination with antifungals was evaluated using the checkerboard method, and drug interactions were assessed by the fractional inhibition concentration index (FICI) and ΔE models. The effects of ASA on mTOR signaling were examined by western blotting. Alkaline phosphatase activity, acridine orange stain assay, and autophagy-related gene expressions were examined to evaluate autophagic activity. Autophagosomes were observed by transmission electron microscopy. Reactive oxygen species (ROS) were detected by DCFH-DA. Mitochondrial membrane potential (MMP), malondialdehyde (MDA), and ATP levels were determined using commercial kits. ASA inhibited C. albicans biofilm in a concentration dependent manner and showed synergistic effects against biofilms when combined with amphotericin B or 5-fluorocytosine. ASA treatment induced oxidative stress, evidenced by increased ROS and MDA levels, alongside a reduction in ATP and MMP. ASA inhibited mTOR signaling and induced autophagy in C. albicans biofilms by increasing oxidative stress and mitochondrial dysfunction, contributing to biofilm inhibition. This study provides valuable insights into the potential of ASA as an adjunct therapy in combination with antifungal agents for managing C. albicans biofilm-related infections.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411060"},"PeriodicalIF":2.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Therapeutic Potential of Cordyceps Mushroom on SARS-CoV-2 Using Virtual Screening against M^pro and In Vitro Validation of Cordycepin. 利用针对 Mpro 的虚拟筛选和虫草素的体外验证，探索冬虫夏草蘑菇对 SARS-CoV-2 的治疗潜力。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-26 DOI: 10.4014/jmb.2411.11063

Mohammad Hassan Baig, Ayman Turk, Preeti Vishwakarma, Yun Seong Jo, Jae-June Dong, Dae Hee Lee, Young Guk Kim, Mi Kyeong Lee, Jae-Yong Cho

{"title":"Exploring the Therapeutic Potential of Cordyceps Mushroom on SARS-CoV-2 Using Virtual Screening against Mpro and In Vitro Validation of Cordycepin.","authors":"Mohammad Hassan Baig, Ayman Turk, Preeti Vishwakarma, Yun Seong Jo, Jae-June Dong, Dae Hee Lee, Young Guk Kim, Mi Kyeong Lee, Jae-Yong Cho","doi":"10.4014/jmb.2411.11063","DOIUrl":"https://doi.org/10.4014/jmb.2411.11063","url":null,"abstract":"Pathogenic coronavirus, including COVID-19, threatens human health, and there have been strong demands for efficient therapeutics. Cordyceps militaris is a medicinal mushroom that has long been used for immune enhancement, anticancer, and antiviral effects. Therefore, the inhibitory potentials of constituents of C. militaris against COVID-19 were analyzed using various virtual screening analyses. Among ten constituents of C. militaris, cordycepin, the major component, and 3'-deoxyuridine and 2'-O-methyl-adenosine showed strong binding affinity to Mpro, a potential target for COVID-19 therapeutics. Considering the structure-activity relationship, nucleosides having deoxyribose and methoxyribose moiety are important for the affinity to Mpro. Cordycepin is also bound to Mpro mutants, and the binding mechanisms between cordycepin and Mpro were investigated further by MD simulation and MM/PBSA analysis. Principal component analysis also confirmed the conformational change of Mpro by cordycepin, which inhibits the function of Mpro. In vitro, the efficacy of cordycepin was measured using Vero cells infected with SARS-CoV-2, which showed excellent inhibition with an IC50 value of 29 μM. Conclusively, the constituents of C. militaris are expected to inhibit SARS-CoV-2 replication through binding to Mpro. Therefore, C. militaris can be an essential therapeutic for coronavirus through the synergistic effect of its constituents.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411063"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tsrp1 Is a Novel Cyclic Diguanosine Monophosphate Effector that Plays a Role in the Organic-Solvent Tolerance of Rhodococcus ruber.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-26 DOI: 10.4014/jmb.2410.10039

Fan Zhang, Han Yu, Lei Ai, Yixin Hao, Ren Peng

{"title":"Tsrp1 Is a Novel Cyclic Diguanosine Monophosphate Effector that Plays a Role in the Organic-Solvent Tolerance of Rhodococcus ruber.","authors":"Fan Zhang, Han Yu, Lei Ai, Yixin Hao, Ren Peng","doi":"10.4014/jmb.2410.10039","DOIUrl":"https://doi.org/10.4014/jmb.2410.10039","url":null,"abstract":"Cyclic diguanosine monophosphate is a ubiquitous second messenger that regulates diverse cellular processes. Rhodococcus ruber SD3 has potential for use in removing environmental pollutants such as phenol and toluene. In this study, Tsrp1 was found to be a novel cyclic diguanosine monophosphate effector in this strain. The interaction between Tsrp1 and c-di-GMP was verified by surface plasmon resonance, and the dissociation constant was 64 ± 6.84 μM. Moreover, in comparison with the wild-type strain, the recombinant R. ruber SD3 strain, which exhibited elevated levels tsrp1 gene expression, demonstrated enhanced growth in the presence of toluene and phenol. Both recombinant R. ruber SD3 and the wild-type strain completely degraded toluene (0.3 g/l, 0.6 g/l and 0.9 g/l) and phenol (0.6 g/l, 0.8 g/l and 1.0 g/l) in 72 h. Furthermore, differential expression of key genes encoding transcription factors was identified based on the transcriptomic comparison between the two strains. This study is the first to describe a novel cyclic diguanosine monophosphate effector and its role in the characteristics of R. ruber SD3, which will shed new light on the mechanisms underlying the organic solvent tolerance of R. ruber SD3.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2410039"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Biosynthesis of Gastrodin by UDP-Glycosyltransferase from Rauvolfia serpentina.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-26 DOI: 10.4014/jmb.2501.01002

Lin Ge, Yu Xia, Wenxin Xu, Ruobing Jia, Tingting Zhang

{"title":"Efficient Biosynthesis of Gastrodin by UDP-Glycosyltransferase from Rauvolfia serpentina.","authors":"Lin Ge, Yu Xia, Wenxin Xu, Ruobing Jia, Tingting Zhang","doi":"10.4014/jmb.2501.01002","DOIUrl":"https://doi.org/10.4014/jmb.2501.01002","url":null,"abstract":"Gastrodin, the primary bioactive constituent of Gastrodia elata, possesses numerous remarkable pharmacological properties. In this investigation, UDP-glycosyltransferase from Rauvolfia serpentina (RsUGT) was expressed, subsequently purified and characterized. The maximum yield of the enzyme was 17.57 mU/ml and possessed a relative molecular weight of approximately 77.7 kDa. Utilizing GST affinity resin, RsUGT was purified 20.8-fold, with an overall recovery rate of 58.6% and specific activity of 79.2 mU/mg. The optimal temperature and pH for RsUGT was identified as 40°C and 10.0, respectively. Notably, 2% DMSO could increase the RsUGT activity by 12.15%. The Michaelis-Menten constants KM and Vmax were determined to be 0.50mM and 171.60 mU/mg. By optimizing the conditions for the enzymatic biosynthesis of gastrodin by RsUGT, the highest gastrodin production was 285.35 mg/l, accompanied by a molar conversion rate of 99.67%. In addition, the conditions of gastrodin biosynthesis by recombinant strain BL-RsUGT were also studied. The highest gastrodin production was 225.99 mg/l, and the corresponding pHBA conversion rate was 98.00%. These findings confirmed the promising potential of RsUGT in the production of gastrodin.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501002"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Potential of Lemon Peel Extracts in Cosmetics: Chemical Composition and Bioactive Properties.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-26 DOI: 10.4014/jmb.2412.12042

Jinqin Huang, Xu Xu, Chang Liu, Liping Liu

{"title":"Exploring the Potential of Lemon Peel Extracts in Cosmetics: Chemical Composition and Bioactive Properties.","authors":"Jinqin Huang, Xu Xu, Chang Liu, Liping Liu","doi":"10.4014/jmb.2412.12042","DOIUrl":"https://doi.org/10.4014/jmb.2412.12042","url":null,"abstract":"In this study, the potential of lemon peel extracts as cosmetic raw materials was explored, with focus on their composition, aroma, antimicrobial, and other bioactivities. Lemon peel essential oil (LPEO), extract (LPE) and absolute oil (LPAO) were prepared by hydrodistillation and organic solvent extraction, respectively. GC/MS analysis indicated that LPEO, LPE, and LPAO contained 22, 39, and 9 components, respectively, with terpenoids being the predominant component. LPE had the highest total flavonoid content and exceeded the total phenolic content. LPEO demonstrated the strongest aroma intensity and persistence, as measured by electronic nose. All three lemon peel extracts showed good antioxidant, anti-tyrosinase, and antimicrobial properties, while inhibition rates exceeded 90% in the experimental concentration range in a dose-dependent manner. Although the antioxidant and antibacterial effects of LPAO were stronger than those of LPEO, the latter had better anti-tyrosinase action. LPEO also demonstrated superior anti-inflammatory effects, with inhibitory rates of 87.79 ± 3.86% and 80.75 ± 2.33% on TNF-α and IL-6 at 1 × 10-2 mg/ml. Moreover, LPEO promoted HaCaT cell migration better than LPE and LPAO, and the healing rate of scratched HaCaT cells treated with LPEO at 1 × 10-2 mg/ml for 12 h was 95.29 ± 3.41%. In addition to its antioxidant and antibacterial properties, the overall performance of LPEO was superior by comparison with LPE and LPAO. In summary, the three extracts can be combined to expand their application as skincare and cosmetic additives with aroma-improving, antioxidant, whitening, antibacterial, anti-inflammatory, and wound-healing activities.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412042"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Activity of Ocimum tenuiflorum against Drug Resistant Bacteria Isolated from Raw Beef. Ocimum tenuiflorum 对从生牛肉中分离出的耐药细菌的抗菌活性。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-26 DOI: 10.4014/jmb.2409.09028

Tasmina Tabassum, Anti Islam, K M Salim Andalib, Barnali Sarker, Mijan Mia, Khondoker Shahin Ahmed, Hemayet Hossain, Ahsan Habib

{"title":"Antibacterial Activity of Ocimum tenuiflorum against Drug Resistant Bacteria Isolated from Raw Beef.","authors":"Tasmina Tabassum, Anti Islam, K M Salim Andalib, Barnali Sarker, Mijan Mia, Khondoker Shahin Ahmed, Hemayet Hossain, Ahsan Habib","doi":"10.4014/jmb.2409.09028","DOIUrl":"https://doi.org/10.4014/jmb.2409.09028","url":null,"abstract":"Recent empirical evidence has acknowledged raw meat, particularly beef, as a significant reservoir for diverse foodborne pathogens and drug-resistant strains, posing severe threat to consumer health. This study aimed to isolate and identify drug-resistant bacteria from raw beef samples, obtained from different butcher shops in Khulna city, Bangladesh, as well as, to determine their susceptibility pattern against Ocimum tenuiflorum extracts. Raw beef samples were randomly collected from various butcher shops, followed by the initial isolation of thirty pure bacterial isolates. Later, 16S rRNA gene amplification and analysis identified twelve distinct bacterial species from those isolates. The antimicrobial susceptibility test results revealed ten of the isolates, including Klebsiella pneumoniae, Aeromonas veronii and Enterobacter hormaechei, to exhibit multidrug resistance pattern. Amoxicillin, nitrofurantoin, and flucloxacillin were found to be ineffective against most isolates. However, the ethanolic extracts of O. tenuiflorum were found effective in inhibiting the growth of eight species at three different concentrations. Subsequent HPLC analysis of O. tenuiflorum reported the presence of five secondary metabolites epicatechin, syringic acid, rutin hydrate, p-coumaric acid, and myricetin as potent contributors to the observed antimicrobial activity. Lastly, in silico binding interaction simulations of the secondary metabolites against five relevant targets predict syringic acid and myricetin to have effective antibacterial properties, primarily mediated by better binding affinity and molecular interactions. Thus, this study identified diverse drug-resistant bacteria in raw beef and provided novel insights into the antibacterial properties of O. tenuiflorum extracts.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2409028"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating Deep Learning Models with Genome-Wide Association Study-Based Identification Enhanced Phenotype Predictions in Group A Streptococcus.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-26 DOI: 10.4014/jmb.2411.11010

Peng-Ying Wang, Zhi-Song Chen, Xiaoguo Jiao, Yun-Juan Bao

{"title":"Integrating Deep Learning Models with Genome-Wide Association Study-Based Identification Enhanced Phenotype Predictions in Group A Streptococcus.","authors":"Peng-Ying Wang, Zhi-Song Chen, Xiaoguo Jiao, Yun-Juan Bao","doi":"10.4014/jmb.2411.11010","DOIUrl":"https://doi.org/10.4014/jmb.2411.11010","url":null,"abstract":"Group A Streptococcus (GAS) is a major pathogen with diverse clinical outcomes linked to its genetic variability, making accurate phenotype prediction essential. While previous studies have identified many GAS-associated genetic factors, translating these findings into predictive models remains challenging due to data complexity. The current study aimed to integrate deep learning models with genome-wide association study-derived genetic variants to predict pathogenic phenotypes in GAS. We evaluated the performance of several deep neural network models, including CNN, ResNet18, LSTM, and their ensemble approach in predicting GAS phenotypes. It was found that the ensemble model consistently achieved the highest prediction accuracy across phenotypes. Models trained on the full 4722-genotype set outperformed those trained on a reduced 175-genotype set, underscoring the importance of comprehensive variant data in capturing complex genotype-phenotype interactions. Performance changes in the reduced 175-genotype set compared to the full-set genotype scenarios revealed the impact of data dimensionality on model effectiveness, with CNN remaining robust, while ResNet18 and LSTM underperformed. Our findings emphasized the potential of deep learning in phenotype prediction and the critical role of data-model compatibility.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411010"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of a New Kosakonia Species for Polyethylene Biodegradation.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-13 DOI: 10.4014/jmb.2411.11058

Jin-Hee Cho, Seung-Do Yun, Hyun-Woo Kim, Min-Ju Seo, Bong Hyun Sung, Soo-Jin Yeom

{"title":"Screening of a New Kosakonia Species for Polyethylene Biodegradation.","authors":"Jin-Hee Cho, Seung-Do Yun, Hyun-Woo Kim, Min-Ju Seo, Bong Hyun Sung, Soo-Jin Yeom","doi":"10.4014/jmb.2411.11058","DOIUrl":"10.4014/jmb.2411.11058","url":null,"abstract":"Polyethylene (PE) is among the most widely used synthetic plastics globally, serving as an essential material in daily life and numerous industries, such as packaging for bottles and food, as well as in the production of toys and pipes. PE is used for various purposes owing to its high durability and low production costs, leading to a steadily increasing demand. However, PE waste is a significant contributor to environmental pollution, posing serious threats to marine and soil ecosystems. Therefore, the efficient decomposition of PE, a synthetic polymer known for its resistance to degradation, using bacteria offers a sustainable and effective method for reusing PE. In this study, we isolated a novel species of Kosakonia, designated Kosakonia cowanii JNU01, from a landfill site, capable of biodegrading PE. K. cowanii JNU01 exhibited the highest cell growth rate in media containing PE, indicating its effectiveness in decomposing PE for use as a sole carbon source in its metabolic pathway. Treatment of PE with K. cowanii JNU01 resulted in the emergence of new chemical functional groups, including hydroxyl, carboxyl, amide, and ether groups, within the inert hydrocarbon structure. Analysis of the PE film treated with K. cowanii JNU01 revealed considerable physical degradation on the film's surface. Additionally, various metabolites released from PE by K. cowanii JNU01 were identified. These findings suggest that K. cowanii JNU01 proves to be an effective candidate bacterium for PE degradation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411058"},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprospecting Antarctic Microorganisms: Investigating Hymenobacter psoromatis PAMC26554 for Biochemical Potential.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-13 DOI: 10.4014/jmb.2412.12010

Hirotake Yamaguchi, Ryoichi Yamada, Jun Hyuck Lee, Tae-Jin Oh

{"title":"Bioprospecting Antarctic Microorganisms: Investigating Hymenobacter psoromatis PAMC26554 for Biochemical Potential.","authors":"Hirotake Yamaguchi, Ryoichi Yamada, Jun Hyuck Lee, Tae-Jin Oh","doi":"10.4014/jmb.2412.12010","DOIUrl":"10.4014/jmb.2412.12010","url":null,"abstract":"Microorganisms from the genus Hymenobacter are known for their distinctive traits, yet their secondary metabolite (SM) production has not been thoroughly investigated. In this study, we examined the biosynthetic potential of SMs from Hymenobacter psoromatis PAMC26554, which was isolated from Antarctic lichen. In silico analysis identified biosynthetic gene clusters (BGCs) for terpenes, non-ribosomal peptide synthetases (NRPSs), and polyketide synthases (PKSs), indicating the strain's potential for novel SM production. Optimization of culture conditions showed that R2A medium at 15°C supported growth. HPLC analysis revealed phenylacetic acid (PAA) as a notable compound, which was characterized by ESI-MS and NMR, marking the first isolation of PAA from the genus Hymenobacter. In addition, bioactivity assays indicated moderate lipase inhibition by PAA, while molecular docking studies revealed stable interactions with the enzyme, demonstrating that hydrogen bonding and π-π stacking contribute to its lipase inhibitory activity. In summary, this research highlights the genus Hymenobacter as a potential source for secondary metabolite discovery, with PAA exemplifying its unexplored biochemical capabilities.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412010"},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative Effects of Escin on Inflammation via Glucocorticoid Receptor (GR) in Atopic Dermatitis (AD) Mouse Model.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-11 DOI: 10.4014/jmb.2410.10025

A Yeon Park, Jung Ok Lee, You Na Jang, Su-Young Kim, Ji Hye Heo, Yu-Jin Kim, Jung Min Lee, Dae Won Yoon, Beom Joon Kim, Joon Seok

{"title":"Ameliorative Effects of Escin on Inflammation via Glucocorticoid Receptor (GR) in Atopic Dermatitis (AD) Mouse Model.","authors":"A Yeon Park, Jung Ok Lee, You Na Jang, Su-Young Kim, Ji Hye Heo, Yu-Jin Kim, Jung Min Lee, Dae Won Yoon, Beom Joon Kim, Joon Seok","doi":"10.4014/jmb.2410.10025","DOIUrl":"10.4014/jmb.2410.10025","url":null,"abstract":"Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disease characterized by intense itching. Escin, derived from Aesculus hippocastanum, has several pharmacological functions, including anti-inflammatory and anti-viral effects, and exhibits glucocorticoid-like actions in inflammatory responses. However, its impact on AD has not been extensively studied. We investigated the anti-inflammatory effects of escin on AD and elucidate its underlying mechanism of actions in the dermatophagoides farinae extract (DFE)-induced AD mouse model. The AD-induced group treated with escin showed a significant reduction in immunoglobulin E (IgE) levels, ear thickness, epidermal thickness, and mast cell infiltration compared to the AD group. Additionally, escin significantly reduced the dermatitis score and the sizes of the spleen and lymph nodes. Notably, escin inhibited the reduction of filaggrin expression induced by DFE, while suppressing the upregulation of thymic stromal lymphopoietin (TSLP), interleukin (IL)-4, IL-13, IL-1β, and tumor necrosis factor (TNF)-α. Escin also significantly suppressed DFE-induced NF-κB expression. Interestingly, pre-treatment with RU486, a glucocorticoid receptor (GR) antagonist, attenuated the therapeutic effects of escin. In line with these findings, escin modulated the IFN-γ/TNF-α-mediated changes in TSLP and filaggrin expression in HaCaT keratinocyte cells. Furthermore, escin inhibited the lipopolysaccharide (LPS)-induced overproduction of nitric oxide (NO), protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and mRNA expression of IL-6 and IL-1β in RAW 264.7 cells. These results indicate that escin may offer therapeutic potential in treating AD through the GR.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2410025"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0