Journal of microbiology and biotechnology最新文献

Iodine Biogeochemical Cycle and Microbial Bioremediation of Radioactive Iodine-129. 碘生物地球化学循环与放射性碘-129的微生物修复

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-26 DOI: 10.4014/jmb.2508.08018

Hwa-Hyung Lee

{"title":"Iodine Biogeochemical Cycle and Microbial Bioremediation of Radioactive Iodine-129.","authors":"Hwa-Hyung Lee","doi":"10.4014/jmb.2508.08018","DOIUrl":"https://doi.org/10.4014/jmb.2508.08018","url":null,"abstract":"Iodine is an essential biophilic element that plays pivotal roles in both environmental systems and human physiology, particularly as a key constituent of thyroid hormones and a regulator of atmospheric ozone. In contrast, its radioactive isotope, iodine-129 (I-129), predominantly generated through anthropogenic nuclear activities, represents a persistent environmental and public health concern. With an exceptionally long half-life of approximately 15.7 million years and high environmental mobility, especially in groundwater, combined with a strong tendency to bioaccumulate in the human thyroid, I-129 poses a disproportionate and long-term radiological hazard in contaminated sites. The biogeochemical cycling of iodine involves intricate interconversions among multiple oxidation states and phases across the lithosphere, hydrosphere, atmosphere, and biosphere. Microorganisms are central to these processes, mediating oxidation, reduction, methylation, accumulation, and sorption. While microbial methylation can increase I-129 mobility via the production of volatile methyl iodide, other microbial pathways, notably biosorption and binding to organic matter, provide promising mechanisms for immobilization and natural attenuation. Microbial bioremediation offers a sustainable and cost-effective alternative or complement to conventional physicochemical methods for managing radioactive contaminants. Strategies such as bioreduction, biosorption, bioaccumulation, and biomineralization exploit the metabolic versatility of microorganisms to alter radionuclide speciation, solubility, and mobility. However, practical application to I-129 remains challenging due to its extreme persistence, environmental variability, and uncertainties in predicting its long-term geochemical fate. Effective management of I-129 contamination will require an integrated, multidisciplinary approach that combines advanced microbial ecology insights, optimized biotechnological processes, and long-term monitoring frameworks.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2508018"},"PeriodicalIF":3.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Luffa cylindrica (L.) Roem Extract on Microglial Activation-Mediated Mild Cognitive Impairment via Regulation of CREB Signaling Pathway. 丝瓜的药效罗麻提取物通过调节CREB信号通路对小胶质细胞激活介导的轻度认知障碍的影响。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-26 DOI: 10.4014/jmb.2506.06049

Joon Park, Yongeun Kim, Jung-Eun Lee, Yun Tai Kim

{"title":"Effects of Luffa cylindrica (L.) Roem Extract on Microglial Activation-Mediated Mild Cognitive Impairment via Regulation of CREB Signaling Pathway.","authors":"Joon Park, Yongeun Kim, Jung-Eun Lee, Yun Tai Kim","doi":"10.4014/jmb.2506.06049","DOIUrl":"https://doi.org/10.4014/jmb.2506.06049","url":null,"abstract":"Neuroinflammation is increasingly recognized as a pivotal contributor to mild cognitive impairment (MCI), with microglial activation playing a central role in this process. While Luffa cylindrica (L.) Roem is known for its anti-inflammatory properties, its effects on MCI and its active components have not been fully elucidated. In this study, we evaluated the anti-inflammatory and neuroprotective effects of Luffa cylindrica extract (LCE) on microglial activation and MCI-like behaviors induced by lipopolysaccharide (LPS). BV2 microglial cells were stimulated with LPS (1 μg/ml) and treated with LCE (25, 50, or 100 μg/ml). Microglial activation was assessed via Griess assay, western blotting, RT-PCR, and ELISA. In vivo, male ICR mice were received LCE (50 or 300 mg/kg) orally for 7 days in combination with intraperitoneal LPS (0.5 mg/kg). Cognitive function was evaluated using passive avoidance and Y-maze tests. The hippocampus was harvested for biochemical analysis. High-performance liquid chromatography (HPLC) was used to identify major bioactive components of LCE. LCE treatment significantly reduced the production of nitric oxide (NO), pro-inflammatory cytokine expression, and inflammation-associated protein levels in BV2 cells. These effects were associated with inhibition of the AKT-GSK3β-CREB signaling pathway. In vivo, oral LCE administration ameliorated LPS-induced cognitive impairment and decreased inflammatory markers in the hippocampus. HPLC analysis identified myricetin as a major component of LCE, which independently exhibited anti-inflammatory effects in microglia. These findings highlight the potential of LCE as a natural therapeutic agent for neuroinflammation-related cognitive impairment, with myricetin contributing to its pharmacological activity.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506049"},"PeriodicalIF":3.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat-Treated Lactiplantibacillus plantarum KM2 Fermentation Ameliorate Muscular Atrophy. 热处理的植物乳杆菌KM2发酵改善肌肉萎缩。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-25 DOI: 10.4014/jmb.2506.06042

Minji Kang, Minkyoung Kang, Moon-Hee Sung, Jong-Hoon Kim, Juyeon Lee, Kwangcheol Casey Jeong, Sangnam Oh

{"title":"Heat-Treated Lactiplantibacillus plantarum KM2 Fermentation Ameliorate Muscular Atrophy.","authors":"Minji Kang, Minkyoung Kang, Moon-Hee Sung, Jong-Hoon Kim, Juyeon Lee, Kwangcheol Casey Jeong, Sangnam Oh","doi":"10.4014/jmb.2506.06042","DOIUrl":"https://doi.org/10.4014/jmb.2506.06042","url":null,"abstract":"Sarcopenia, a progressive loss of skeletal muscle mass and function, poses a significant health concern in aging populations and cancer patients. Despite ongoing pharmaceutical research, including drug repurposing strategies, no FDA-approved treatment is currently available for sarcopenia, highlighting the need for safer, food-derived interventions. This study evaluated the anti-aging and muscle-preserving effects of KLP_KM2, a postbiotic formulation derived from Lactiplantibacillus plantarum KM2, using Caenorhabditis elegans and C2C12 muscle cell models. In C. elegans, KLP_KM2 and its components significantly extended lifespan, reduced lipofuscin accumulation, enhanced pharyngeal pumping, and preserved coordinated movement patterns. These effects were accompanied by upregulation of longevity, immune/stress response, and muscle function-related genes. In C2C12 myotubes, KLP_KM2 treatment mitigated CT26-conditioned medium-induced muscle atrophy, restoring myotube diameter and length, increasing expression of myogenic markers (MyoD, myogenin, MHC I, MHC IIa), and downregulating atrophy markers (Atrogin-1, MuRF1). These findings suggest that KLP_KM2 may serve as a promising postbiotic intervention to support muscle health, prevent sarcopenia, and counteract cancer cachexia. Further in vivo mammalian studies and clinical trials are warranted to validate its therapeutic potential.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506042"},"PeriodicalIF":3.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Mechanistic Anti-Obesity Effects of Red Okra and Diospyros lotus Mixture via Fat Binding and AMPK-Mediated Lipid Metabolism. 红秋葵和黄花莲子混合物通过脂肪结合和ampk介导的脂质代谢的双机制抗肥胖作用

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-24 DOI: 10.4014/jmb.2506.06030

Yeo-Jin Choi, Wan Seok Kang, Eun Kim, Seon Ah Son, Ki Hoon Lee, Youngbae Kim, Jin Seok Kim, Sunoh Kim

{"title":"Dual Mechanistic Anti-Obesity Effects of Red Okra and Diospyros lotus Mixture via Fat Binding and AMPK-Mediated Lipid Metabolism.","authors":"Yeo-Jin Choi, Wan Seok Kang, Eun Kim, Seon Ah Son, Ki Hoon Lee, Youngbae Kim, Jin Seok Kim, Sunoh Kim","doi":"10.4014/jmb.2506.06030","DOIUrl":"https://doi.org/10.4014/jmb.2506.06030","url":null,"abstract":"Obesity is a major global health issue associated with metabolic dysfunctions including dyslipidemia, insulin resistance, and hepatic steatosis. This study investigated the dual anti-obesity mechanisms of a combined extract of red okra (Abelmoschus esculentus L. Moench, RO) and Diospyros lotus L. leaves (DL) in a high-fat diet (HFD)-induced obese mouse model. Fat-binding capacity (FBC) analysis revealed that RO exhibited significantly higher FBC than DL, suggesting a greater potential to inhibit intestinal fat absorption. In vitro, RO more effectively suppressed lipid accumulation in 3T3-L1 adipocytes, whereas DL enhanced lipolytic activity by stimulating glycerol release from differentiated adipocytes. To optimize the complementary functions of both extracts, various mixing ratios were evaluated, and the 4:1 ratio (RODLTM) was identified as optimal. This combination resulted in approximately 25% reduction in lipid accumulation, a significant increase in glycerol release, and a 39% elevation in fecal fat excretion. In HFD-fed mice, oral administration of the RODL mixture led to up to 14% suppression in body weight gain, improved serum triglyceride and cholesterol levels, and alleviated hepatic steatosis. Mechanistically, the extract combination activated AMPKα signaling, leading to the upregulation of lipolysis-related proteins (ATGL, pHSL), thermogenesis-related markers (UCP1, PGC1α), and fatty acid oxidation regulators (PPARα, CPT1, ACOX1). These findings indicate that the RO and DL extract combination exerts anti-obesity effects through both inhibition of fat absorption and modulation of lipid metabolic pathways. This dual mechanism supports its potential as a safe and effective natural therapeutic for obesity prevention and management.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506030"},"PeriodicalIF":3.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastrointestinal Tolerance and Gut Microbiota Modulation of Encapsulated and Free Forms of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis. 包封型和游离型嗜酸乳杆菌和动物双歧杆菌的胃肠道耐受性和肠道菌群调节。lactis。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-24 DOI: 10.4014/jmb.2506.06028

Geonhee Kim, Hyunbin Seong, Seung Hee Han, Hwa Rin Kim, Sung-Hwan Kim, Hye-Jin Ku, Hye-Jung Han, Chul-Hong Kim, Nam Soo Han

{"title":"Gastrointestinal Tolerance and Gut Microbiota Modulation of Encapsulated and Free Forms of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis.","authors":"Geonhee Kim, Hyunbin Seong, Seung Hee Han, Hwa Rin Kim, Sung-Hwan Kim, Hye-Jin Ku, Hye-Jung Han, Chul-Hong Kim, Nam Soo Han","doi":"10.4014/jmb.2506.06028","DOIUrl":"https://doi.org/10.4014/jmb.2506.06028","url":null,"abstract":"The viability and efficacy of probiotics are strongly influenced by their delivery form, especially under harsh gastrointestinal conditions. This study assessed the gastrointestinal resistance of encapsulated and free from Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis, and evaluated the specific effects of digested free from strains on the gut microbiome. An in vitro digestion model simulating gastric and intestinal conditions was used to assess probiotic survival, followed by fecal fermentation to examine microbial and metabolic responses. The encapsulated probiotics, formulated with a multilayer matrix, demonstrated significantly higher viability and preserved membrane integrity than the free forms. Despite reduced viability, free from probiotics modulated the gut microbiota. Both strains promoted colonization of beneficial genera such as Bifidobacterium, Lactobacillus, and Prevotella, while maintaining microbial balance without inducing dysbiosis. Additionally, probiotic supplementation enhanced short-chain fatty acid production, particularly acetate and butyrate, indicating increased microbial fermentation activity. Notably, distinct ecological patterns emerged between the two strains, with L. acidophilus inducing dynamic shifts and recovery, and B. lactis contributing to structural stability. These findings underscore the importance of strain-specific selection and encapsulation strategies in developing targeted and effective probiotic interventions.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506028"},"PeriodicalIF":3.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coexistence of Multidrug Resistance and Hypervirulence-Associated Genes in Clinical Carbapenem-Resistant Pseudomonas aeruginosa. 耐碳青霉烯铜绿假单胞菌多药耐药与高毒力相关基因共存的研究

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-24 DOI: 10.4014/jmb.2505.05031

Hye Hyun Cho, Yumi Park

{"title":"Coexistence of Multidrug Resistance and Hypervirulence-Associated Genes in Clinical Carbapenem-Resistant Pseudomonas aeruginosa.","authors":"Hye Hyun Cho, Yumi Park","doi":"10.4014/jmb.2505.05031","DOIUrl":"https://doi.org/10.4014/jmb.2505.05031","url":null,"abstract":"The recent worldwide emergence of hypervirulent, multidrug-resistant Pseudomonas aeruginosa represents a critical public health threat. The molecular typing, multidrug resistance (MDR) rates, and hypervirulence-associated genes in carbapenem-resistant P. aeruginosa (CRPA) isolates can vary across geographic locations and patients, highlighting their clinical significance. In this study we investigated the epidemiology and relationship between MDR and the presence of hypervirulence-associated genes in CRPA isolates. Accordingly, we performed antimicrobial susceptibility tests, multilocus sequence typing (MLST), and PCR-based detection of carbapenemase and virulence-associated genes. Notably, 34.9% (139/398) of the isolates were carbapenem-resistant, with 79.1% of these exhibiting MDR. Of the 30 sequence types (STs) identified by MLST, ST773 was the most prevalent (28.8%), followed by ST235 (23.0%). New Delhi metallo-β-lactamase (NDM)-1 or imipenemase-6 occurred in 45.3% of the CRPA strains. Common virulence genes included exoT (100.0% of isolates), exoY (95.7%), exoU (66.2%), and exoS (34.5%). exoU was associated with antibiotic resistance (p<0.05), except for carbapenems in CRPA isolates. The exoU/exoT/exoY Type III secretion system (T3SS) genotype was the most prevalent among the carbapenemase-producing CRPA strains. Among the 139 CRPA isolates, we identified a pandrug-resistant, NDM-1-producing ST235 strain co-expressing exoS and exoU. This study highlights the important association between exoU and MDR, indicating a potential relationship between T3SS genotypes and antibiotic resistance. Additionally, the identification of a hypervirulent, pandrug-resistant P. aeruginosa ST235 isolate harboring blaIMP-6 underscores the critical need for enhanced surveillance against high-risk strains.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505031"},"PeriodicalIF":3.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Regulation of the allF Operon by ArcA and AllS Enables Anaerobic Allantoin Utilization in Escherichia coli. ArcA和all对allF操纵子的双重调控使大肠杆菌厌氧利用尿囊素。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-24 DOI: 10.4014/jmb.2507.07057

Nam Yeun Kim, Ok Bin Kim

{"title":"Dual Regulation of the allF Operon by ArcA and AllS Enables Anaerobic Allantoin Utilization in Escherichia coli.","authors":"Nam Yeun Kim, Ok Bin Kim","doi":"10.4014/jmb.2507.07057","DOIUrl":"https://doi.org/10.4014/jmb.2507.07057","url":null,"abstract":"During anaerobic growth, Escherichia coli is capable of utilizing allantoin as its sole nitrogen source. Allantoin, a purine derivative, is initially degraded into ureidoglycolate with the release of two NH3. Ureidoglycolate can then enter either the glycerate branch (to 2-phosphoglycerate) or the oxamate branch (releasing additional NH3). In the oxamate branch, ureidoglycolate first is oxidized to oxalurate, then converted to oxamate and carbamoyl phosphate by oxamic transcarbamylase (OXTCase); carbamoyl phosphate, in turn is used by carbamate kinase (CK) to generate ATP and NH3. This study focuses on the transcriptional regulation of OXTCase and CK, which catalyze the final two steps of the oxamate branch and are encoded by the allFGHK operon (allF operon), the most recently identified genes in the allantoin pathway. Transcription of the allF operon was analyzed using a plasmid-borne allF'-'lacZ reporter and relevant regulator mutants. High expression of the allF operon under anaerobiosis with allantoin requires the local regulator AllS and the global regulator ArcA. EMSA confirmed the direct binding of AllS and ArcA to the promoter of allF. These findings indicate that the oxamate branch is directly regulated by the activator AllS, one of two local regulators (AllR and AllS) in the allantoin pathway. Furthermore, we identified ArcA as the activator of allF operon transcription under anaerobic conditions. Although allantoin degradation is known to occur only anaerobically, the regulator remains unidentified. Our findings demonstrate ArcA's involvement. ArcA, the regulator of the allF operon, may also control other anaerobic genes in the allantoin cluster, directly or indirectly.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507057"},"PeriodicalIF":3.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Standardizing Bacterial Extracellular Vesicle Purification: A Call for Consensus. 标准化细菌细胞外囊泡净化：呼吁达成共识。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-23 DOI: 10.4014/jmb.2506.06011

Dongsic Choi, Eun-Young Lee

{"title":"Standardizing Bacterial Extracellular Vesicle Purification: A Call for Consensus.","authors":"Dongsic Choi, Eun-Young Lee","doi":"10.4014/jmb.2506.06011","DOIUrl":"https://doi.org/10.4014/jmb.2506.06011","url":null,"abstract":"Bacteria constitutively produce extracellular vesicles (EVs), which play a pivotal role in linking bacterial physiology to therapeutics. Despite significant advances over the past decade, critical challenges persist in isolating EVs due to the absence of standardized purification methods. While the International Society for Extracellular Vesicles provides well-defined minimal guidelines for human EVs, bacterial EV research still faces inconsistencies across laboratories, limiting reproducibility and broader utility. This review provides an in-depth overview of technical aspects of bacterial EV purification and gives future perspectives for achieving high-purity and reproducibility when isolating EVs from diverse bacterial species. It further addresses the necessity of distinguishing bacterial EVs from host-derived vesicles and potential contaminants, particularly in clinical and microbiota contexts. Establishing gold-standard protocols and defining bona fide bacterial EVs in parallel with the rigor seen in human EV studies will advance our understanding of their roles in host-microbe interactions, microbiota dysbiosis, and therapeutic potential.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506011"},"PeriodicalIF":3.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SHR02 Modulates Inflammatory and Oxidative Stress Responses by Inhibiting NF-κB and Activating Nrf2 in Macrophages and Dendritic Cells. SHR02通过抑制NF-κB和激活Nrf2调节巨噬细胞和树突状细胞的炎症和氧化应激反应。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-23 DOI: 10.4014/jmb.2506.06020

Hien Thi Thu Do, Chaelin Lee, Inmoo Rhee

引用次数: 0

The Effect of Oral Probiotic Bacillus amyloliquefaciens on Intestinal Microbiota, Intestinal Structure, Serum Antioxidant Capacity and Inflammatory Responses of Heat Stressed Rats. 口服解淀粉芽孢杆菌对热应激大鼠肠道菌群、肠道结构、血清抗氧化能力和炎症反应的影响

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-23 DOI: 10.4014/jmb.2505.05008

Xiaodong Zhang, Li Tang, Bluefin Masell Freeman, Baikui Wang, Wenying Shen, Weifen Li

{"title":"The Effect of Oral Probiotic Bacillus amyloliquefaciens on Intestinal Microbiota, Intestinal Structure, Serum Antioxidant Capacity and Inflammatory Responses of Heat Stressed Rats.","authors":"Xiaodong Zhang, Li Tang, Bluefin Masell Freeman, Baikui Wang, Wenying Shen, Weifen Li","doi":"10.4014/jmb.2505.05008","DOIUrl":"https://doi.org/10.4014/jmb.2505.05008","url":null,"abstract":"This study investigated the effect of Bacillus amyloliquefaciens (BaSC06) in alleviating heat stress in rats. The rats were randomly divided into four groups. Each rat in Group 1 and Group 3 received oral 1 ml/day of PBS, whereas those in Group 2 and Group 4 were administered 1 ml/day of BaSC06 (108 CFU/ml). After two weeks, the rats in Groups 3 and 4 were exposed to heat stress at 42°C for 30 min, while those in Groups 1 and 2 were maintained at 25°C. The result showed that oral BaSC06 increased abundance of Firmicutes phylum and Ruminococcaceae Family along with reduced level of Bacteroidetes family. Compared to Group 1, the height of small intestine villi increased significantly (P < 0.05) in Group 2, and intestinal villus height and mucosal thickness decreased significantly in Group 3 than that in Group 4 (P < 0.05). The distribution of ZO-1 was disrupted in Group 3 which was ameliorated in Group 4. The number of bacteria in the liver, spleen and mesenteric lymph nodes was significantly higher in Group 3 than other groups (P < 0.05) meanwhile the LPS level in Group 4 decreased significantly compared to Group 3 (P < 0.05). The levels of IL-1, IL-10 , HSP70 and MDA increased significantly (P < 0.05), the activity of CAT decreased significantly (P < 0.05) in Group 3 compared to the other groups. The adverse effects of heat stress can be alleviated by gavage BaSC06, which improve the composition of intestinal microbiota. It is helpful to elucidate the mechanism of probiotics to counteract the negative effects of heat stress.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505008"},"PeriodicalIF":3.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0