Journal of microbiology and biotechnology最新文献

Effects of Acute Abdomen III on Sepsis-Induced Intestinal Damage in a Rat Model. 急腹症III对脓毒症引起的大鼠肠道损伤的影响。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-26 DOI: 10.4014/jmb.2412.12067

Lu Wang, Jingjing Hu, Fangyu Hu, Yuexuan Chen, Ming Fang, Yuanling Ye

{"title":"Effects of Acute Abdomen III on Sepsis-Induced Intestinal Damage in a Rat Model.","authors":"Lu Wang, Jingjing Hu, Fangyu Hu, Yuexuan Chen, Ming Fang, Yuanling Ye","doi":"10.4014/jmb.2412.12067","DOIUrl":"https://doi.org/10.4014/jmb.2412.12067","url":null,"abstract":"The disruptions to intestinal integrity contribute to sepsis-related complications. Acute abdomen III is a traditional Chinese medicine formula. The objective of this research is to investigate the impact of acute abdomen III upon sepsis-induced intestinal damage. A rat model of cecal ligation and puncture was used to evaluate the impact of acute abdomen III on sepsis-induced intestinal damage. Histopathological analysis of intestinal tissue damage, detection of systemic inflammation and measurement of tight junction protein were performed by hematoxylin and eosin staining, enzyme-linked immunosorbent assay, and Western blot, respectively. Oxidative stress and intestinal barrier integrity were assessed. Terminal deoxynucleotidyl transferase dUTP nick end labeling was employed to evaluate apoptosis. Expression levels of the apoptosis-related proteins were examined. Model rats treated with acute abdomen III exhibited significantly mitigated intestinal damage. Acute abdomen III treatment reduced systemic inflammation and oxidative stress, as evidenced by downregulation of malondialdehyde and upregulation of superoxide dismutase and catalase. Acute abdomen III therapy lowered endotoxin, D-lactate, and diamine oxidase levels, while boosting the levels of tight junction proteins ZO-1, claudin-1 and occludin, implying an enhancement in intestinal barrier integrity. Acute abdomen III also markedly suppressed apoptosis in intestinal epithelial cells. Acute abdomen III can protect against sepsis-induced intestinal damage by reducing systemic inflammation and oxidative stress while promoting intestinal barrier integrity.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412067"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Loading Proximity Ligation-Induced PCR Technique for Fluorescent Detection of Intact Methicillin-Resistant Staphylococcus aureus. 完整耐甲氧西林金黄色葡萄球菌的表面负载接近连接诱导PCR荧光检测技术。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-23 DOI: 10.4014/jmb.2504.04004

Tingting Li, Yang Liu, Meijia Zhao, Xue Xin, Shuhong Jin

{"title":"Surface Loading Proximity Ligation-Induced PCR Technique for Fluorescent Detection of Intact Methicillin-Resistant Staphylococcus aureus.","authors":"Tingting Li, Yang Liu, Meijia Zhao, Xue Xin, Shuhong Jin","doi":"10.4014/jmb.2504.04004","DOIUrl":"https://doi.org/10.4014/jmb.2504.04004","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA)-induced pneumonia in nursing patients with chronic obstructive pulmonary disease (COPD) necessitates rapid detection, timely intervention, and meticulous clinical management. Thus, the development of sensitive and accurate techniques for MRSA detection holds significant clinical nursing of infectious diseases. In this study, we designed a surface loading proximity ligation assay (PLA) for the precise detection of MRSA in pulmonary infections by simultaneously targeting three characteristic proteins. This assay utilizes three customized probes: the first probe targets protein A on the MRSA surface, the second probe immobilizes on the biological lipid layer, and the third probe identifies PBP2a (a protein responsible for drug resistance of MRSA). The proposed strategy integrates proximity ligation of these three probes with polymerase chain reaction (PCR) to perform \"AND\" logic-based analysis of the three key MRSA components, enabling sensitive detection of intact MRSA. Taking advantage of the high signal amplification efficiency of PCR and elevated target recognition capability of PLA, the method exhibited a low limit of detection of 2.9 CFU/ml. As a result, the proposed method demonstrated significantly improved accuracy for MRSA detection. We believe this novel integrated strategy could diversify existing bacterial detection approaches and may inspire the development of promising drug candidates in COPD nursing.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504004"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schisandrin C Improves Chronic Stress-Induced Dyslipidemia in Mice by Regulating Pyroptosis and Autophagy Levels. 五味子素C通过调节焦亡和自噬水平改善小鼠慢性应激性血脂异常。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-23 DOI: 10.4014/jmb.2501.01041

Yuling Liu, Wenjun Lu, Yue Huang, Chaozhi Xu, Wanhua Wu, Zongyi Zhang, Yulin Zhang, Shanqian Li, Guixian Wu, Doudou Wang, Yao Xu, Meimei Zheng, Hongxian Wu, Lina Hu

{"title":"Schisandrin C Improves Chronic Stress-Induced Dyslipidemia in Mice by Regulating Pyroptosis and Autophagy Levels.","authors":"Yuling Liu, Wenjun Lu, Yue Huang, Chaozhi Xu, Wanhua Wu, Zongyi Zhang, Yulin Zhang, Shanqian Li, Guixian Wu, Doudou Wang, Yao Xu, Meimei Zheng, Hongxian Wu, Lina Hu","doi":"10.4014/jmb.2501.01041","DOIUrl":"https://doi.org/10.4014/jmb.2501.01041","url":null,"abstract":"Chronic stress emerges as a significant risk factor for metabolic disorders, including hyperlipidemia and fatty liver disease. Schisandrin C (SchC), an essential component of Schisandra chinensis lignans, is known to possess lipid-lowering and liver-protective properties. However, the precise mechanisms underlying its action remain incompletely understood. We hypothesized that SchC exerts a protective effect on chronic stress-induced dyslipidemia in mice by modulating hepatic autophagy and pyroptosis. In this study, a dual model was established for 12 weeks, combining chronic stress with a high-fat diet (HFD). SchC or simvastatin were given respectively starting at week 10. Blood, epididymal white adipose tissue and liver were collected for further analysis. Behavioral tests and lipid levels showed that the dual model construction of HFD and chronic stress used in this study was successful in placing mice in a stressful environment and triggering dyslipidemia. SchC intervention effectively impeded the accumulation of eWAT and reduced the increased lipid levels in mice induced by chronic stress. The expression levels of the pyroptosis marker Caspase-1 and other inflammatory factors NLRP3, IL-1β, and IL-18 were significantly reduced in the liver tissues of chronically stressed mice under SchC treatment. Additionally, SchC significantly reduced expression levels of autophagy-related factors such as Beclin-1, the LC3-B/A ratio, P62, and markers related to the autophagy pathway (PI3K/AKT/mTOR). SchC effectively improves dyslipidemia through various pathways including inhibiting the PI3K/AKT/mTOR pathway, promoting autophagy, reducing pyroptosis. This provides a solid theoretical foundation for the clinical application of SchC in the treatment of dyslipidemia and related diseases.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501041"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic Plantaricins Derived from Lactiplantibacillus plantarum KM2 Induce Cell Lysis of Listeria monocytogenes. 植物乳杆菌KM2合成植物素诱导单核增生李斯特菌细胞裂解。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-23 DOI: 10.4014/jmb.2504.04006

Seung-Eun Oh, Sojeong Heo, Minkyeong Kim, Yura Moon, Sumin Lee, Chaerin Park, Huieun Sung, Gawon Lee, Jina Kim, Moon-Hee Sung, Do-Won Jeong

{"title":"Synthetic Plantaricins Derived from Lactiplantibacillus plantarum KM2 Induce Cell Lysis of Listeria monocytogenes.","authors":"Seung-Eun Oh, Sojeong Heo, Minkyeong Kim, Yura Moon, Sumin Lee, Chaerin Park, Huieun Sung, Gawon Lee, Jina Kim, Moon-Hee Sung, Do-Won Jeong","doi":"10.4014/jmb.2504.04006","DOIUrl":"https://doi.org/10.4014/jmb.2504.04006","url":null,"abstract":"Listeria monocytogenes is a deadly foodborne pathogen that presents significant challenges in food safety management due to its high resilience in various environments. This study evaluated antibacterial activities of synthetic plantaricins derived from Lactiplantibacillus plantarum KM2 against L. monocytogenes. The minimum inhibitory concentrations of the five synthetic plantaricin combinations-spPlnA, spPlnJ, spPlnE&F, spPlnE&J, and spPlnJ&K-were 1.4, 1.5, 1.8, 1.6, and 1.6 μg/ml, respectively, Raman spectroscopy and transmission electron microscopy demonstrated that the synthetic plantaricins induced morphological alterations, including cell wall damage and cell lysis. Notably, spPlnE&F and spPlnE&J were shown to effectively disrupt the bacterial cell wall. Furthermore, assessments of antibacterial stability under varying temperatures and pH conditions showed that plantaricin combinations maintained their efficacy at pH levels ranging from 4 to 7 and temperatures below 40°C. These findings suggest that synthetic plantaricins have strong potential as natural preservatives in food applications, offering an effective approach to targeting specific pathogens and enhancing food safety.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504006"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between Serum Uric Acid Levels and Salivary Microbiota in Patients with Obstructive Sleep Apnea. 阻塞性睡眠呼吸暂停患者血清尿酸水平与唾液微生物群的关系

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-23 DOI: 10.4014/jmb.2503.03042

Yujia Lu, Wanxin Zhang, Min Yu, Xuehui Chen, Chunyan Liu, Xuemei Gao

{"title":"Association between Serum Uric Acid Levels and Salivary Microbiota in Patients with Obstructive Sleep Apnea.","authors":"Yujia Lu, Wanxin Zhang, Min Yu, Xuehui Chen, Chunyan Liu, Xuemei Gao","doi":"10.4014/jmb.2503.03042","DOIUrl":"https://doi.org/10.4014/jmb.2503.03042","url":null,"abstract":"The microbiota is associated with obstructive sleep apnea (OSA) and hyperuricemia (HUA), but the relationship between oral microbiota and OSA-related HUA remains unclear. Our study investigated salivary microbiota differences between individuals with OSA and those with both OSA and HUA, and explored the link between oral microbiome alterations and uric acid fluctuations in OSA patients. Seventy-two adults were divided into four groups: controls (n = 20, 33.75 ± 9.46 years), OSA (n = 23, 44.08 ± 13.70 years), OSA with comorbid HUA (OSA+HUA, n = 22, 40.18 ± 9.58 years), and OSA with medication-controlled HUA (n = 7, 44.56 ± 15.14 years). Salivary microbiota and proteomic profiles were analyzed using 16S rRNA sequencing and Astral DIA. OSA and OSA+HUA showed reduced alpha-diversity compared to controls. The OSA+HUA group had increased Oribacterium abundance relative to the OSA group, which decreased after uric acid treatment, whereas Rothia, Capnocytophaga, and Aggregatibacter showed the opposite trend. 104 differentiated proteins were identified between the OSA and OSA+HUA groups. Oribacterium was positively correlated with several antioxidant proteins, while the other three genera were negatively correlated. This study identifies non-invasive biomarkers in the OSA+HUA group, as the first of its kind, highlighting the role of oral microbiota in future research and therapies.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503042"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Makgeolli Lees as a Novel Prebiotic Candidate: Effects on Human Gut Microbiota and Metabolites. 米酒作为一种新的候选益生元：对人类肠道微生物群和代谢物的影响。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-23 DOI: 10.4014/jmb.2504.04020

Jun Hoi Kim, Hwa Rin Kim, Hyunbin Seong, Seung Hee Han, Geonhee Kim, Yuri Choi, Nam Soo Han

{"title":"Makgeolli Lees as a Novel Prebiotic Candidate: Effects on Human Gut Microbiota and Metabolites.","authors":"Jun Hoi Kim, Hwa Rin Kim, Hyunbin Seong, Seung Hee Han, Geonhee Kim, Yuri Choi, Nam Soo Han","doi":"10.4014/jmb.2504.04020","DOIUrl":"https://doi.org/10.4014/jmb.2504.04020","url":null,"abstract":"Makgeolli lees (ML), an underutilized byproduct of traditional Korean rice wine production, contains abundant indigestible carbohydrates and microbial residues. This study evaluates the prebiotic potential of ML using a simulated human digestion and in vitro fecal fermentation model. In vitro digestion showed that 61.30% of total carbohydrates and 89.92% of crude protein remained undigested. During in vitro fecal fermentation, treatment with ML induced compositional changes in the gut microbiota, including increased abundances of Bifidobacterium bifidum (Δ 0.37 ± 0.09%), Prevotella copri (Δ 0.98 ± 0.24%), and Bacteroides uniformis (Δ 0.46 ± 0.06%), along with a decrease in Bacteroides ovatus (Δ -2.14 ± 1.51%). In addition, SCFA concentrations were elevated after 12 h of fermentation, with acetate at 84.80 ± 4.88 mM, propionate at 43.75 ± 2.74 mM, and butyrate at 20.22 ± 0.71mM. The alterations in microbial composition and associated metabolite profiles were comparable to those observed following fructooligosaccharides (FOS) supplementation. These findings indicate that ML function as a fermentable substrate that selectively enriches health-associated gut microbiota and enhances the production of beneficial microbial metabolites, thereby supporting their potential application as a sustainable and cost-effective prebiotic candidate in dietary applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504020"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modified Renshen Wumei Decoction Improves Qi-Yin Deficiency Diarrhea by Regulating the Gut Microbiotas and Metabolites in Rats. 加味人参乌梅汤通过调节肠道菌群及代谢产物改善大鼠气阴虚腹泻。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-23 DOI: 10.4014/jmb.2412.12037

Junqi Zhao, Zhiwei Guan, Shuhua Fan, Jianli Qiu, Yan Xu, Qiong Zhao

{"title":"Modified Renshen Wumei Decoction Improves Qi-Yin Deficiency Diarrhea by Regulating the Gut Microbiotas and Metabolites in Rats.","authors":"Junqi Zhao, Zhiwei Guan, Shuhua Fan, Jianli Qiu, Yan Xu, Qiong Zhao","doi":"10.4014/jmb.2412.12037","DOIUrl":"https://doi.org/10.4014/jmb.2412.12037","url":null,"abstract":"The hub metabolites and hub microorganisms that play an important role in the intervention of Qi-yin deficiency syndrome with diarrhea by Modified Renshen Wumei Decoction (MRWD) are still unclear. Therefore, we explored it based on multi-omics analysis. A total of 32 Sprague-Dawley rats were collected and randomly allocated into different groups. Subsequently, blood samples and fecal samples were collected from all 32 rats for non-targeted metabolome sequencing and 16S rRNA gene sequencing respectively. Among them, fecal samples of 24 rats were also used for targeted metabolomics sequencing (6 rats in each group). Pathological findings, and D-lactate, diamine oxidase (DAO), aquaporin 3 (AQP3), and aquaporin 8 (AQP8) levels in serum and colon samples were evaluated after the 21-day trial, and the results showed that diarrhea caused intestinal injury, which was ameliorated by infusion of MRWD. Based on multi-omics sequencing analysis, five microorganisms were superior to the positive control in MRWD intervention. Moreover, Eisenbergiella, Corynebacterium, and unidentified Oscillospiraceae exhibited significant discriminatory capabilities between groups C and D, groups B and C, as well as groups A and C; thus they were identified as hub microorganisms. On the other hand, significant differences in metabolites were observed among different groups with respect to the metabolome. These metabolites exhibited significant enrichment in metabolic pathways such as Butanoate metabolism, Propanoate metabolism, and Pyruvate metabolism. Further correlation analysis revealed that 2-Methylbutyrate was identified as a hub metabolites associated with blood and fecal microorganisms. Moreover, there were complex regulatory relationships between these hub microbes and hub metabolites.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412037"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EIF4A3-Induced Circ_0092278 Enhances Papillary Thyroid Cancer Cell Malignancy by the PI3K/Akt/mTOR Signaling Pathway. eif4a3诱导的Circ_0092278通过PI3K/Akt/mTOR信号通路增强甲状腺乳头状癌细胞恶性。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-19 DOI: 10.4014/jmb.2501.01010

Mengjiang Liu, Linghui Zhang, Zhaodan Yan

{"title":"EIF4A3-Induced Circ_0092278 Enhances Papillary Thyroid Cancer Cell Malignancy by the PI3K/Akt/mTOR Signaling Pathway.","authors":"Mengjiang Liu, Linghui Zhang, Zhaodan Yan","doi":"10.4014/jmb.2501.01010","DOIUrl":"10.4014/jmb.2501.01010","url":null,"abstract":"Circular RNA (circRNA) plays a regulatory role in cancer progression, but the role of circ_0092278 in papillary thyroid cancer (PTC) is unclear. In this research, we aimed to reveal the effect of circ_0092278 on PTC progression as well as its interaction with the eukaryotic translation initiation factor 4A3 (EIF4A3). The expression of circ_0092278 in PTC samples was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). Cell experiments were conducted to assess the effects of circ_0092278 on PTC cell viability, migration, and invasion. Furthermore, key proteins involved in the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway were examined using western blotting. Additionally, a xenograft model in nude mice was used to investigate the in vivo role of circ_ 0092278. The interactions between EIF4A3 and circ_0092278 were analyzed through RNA-binding protein immunoprecipitation and qRT-PCR assays. Circ_0092278, located in the cell cytoplasm, was significantly upregulated in PTC. Knockdown of circ_0092278 reduced PTC cell proliferation, migration, invasion, and tumor growth by inhibiting the PI3K/Akt/mTOR pathway. Conversely, overexpression of circ_0092278 prompted PTC cell malignancy by activating the PI3K/Akt/mTOR pathway. Additionally, EIF4A3 was found to bind to MINK1 (circ_0092278 linear gene), thereby enhancing the expression of circ_0092278. Circ_0092278, controlled by EIF4A3, promotes PTC progression by activating the PI3K/Akt/mTOR pathway. Our findings indicate that targeting the EIF4A3-circ_0092278 axis may provide a novel approach for treating PTC.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501010"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digestibility and Quality Characteristics of Sulgidduk (a Traditional Korean Rice Cake) Prepared with Malic Acid-Treated Wheat Starch. 用苹果酸处理过的小麦淀粉制作的苏吉德年糕的消化率和品质特性

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-18 DOI: 10.4014/jmb.2505.03037

Gyeong A Jeong, Inae Lee, Chang Joo Lee

{"title":"Digestibility and Quality Characteristics of Sulgidduk (a Traditional Korean Rice Cake) Prepared with Malic Acid-Treated Wheat Starch.","authors":"Gyeong A Jeong, Inae Lee, Chang Joo Lee","doi":"10.4014/jmb.2505.03037","DOIUrl":"10.4014/jmb.2505.03037","url":null,"abstract":"With rising obesity rates worldwide and growing health awareness, the demand for low-calorie, blood sugar-controlling products is heightening. Particularly, research has increasingly explored the incorporation of resistant starch (RS) in dietary applications, as it does not add to calorie intake and helps regulate blood sugar levels, prevent constipation, and increase fecal volume, similar to dietary fiber. This study evaluated the quality characteristics and digestibility of malic acid-treated wheat starch (MA starch) with a high RS content for use as a rice flour substitute in Sulgidduk. MA starch was incorporated at 10-40% of the rice flour weight. Adding MA starch to Sulgidduk resulted in slight color changes, with decreased lightness and increased redness and yellowness, although structurally it remained unchanged. Higher MA levels reduced the pH and moisture content but increased sugar content and hardness. Compared to the control sample (rapidly digestible starch (RDS): 68.3%, RS: 30.3%), Sulgidduk prepared with MA starch had lower RDS (55.7%) and higher RS (42.3%) content. MA starch significantly affected RDS and RS levels, which are associated with the glycemic index, while also affecting moisture content and textural characteristics. Based on these findings, 30% substitution with MA starch (MA-30) appears to be the optimal ratio for maintaining health benefits while minimizing quality degradation. Collectively, these results highlight the potential of MA starch as a functional food ingredient and support its application in the formulation of dietary products for calorie management.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503037"},"PeriodicalIF":2.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Biohydrogen Production through Dark Fermentation by Humic Acid: Insights into Microbial Composition and Functional Genes. 腐植酸暗发酵增强生物制氢：微生物组成和功能基因的研究。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-06-17 DOI: 10.4014/jmb.2412.12071

Liguo Zhang, Yanan Bai, Jing Sang, Jinru Dong, Xiujuan Wu, Qiaoying Ban

{"title":"Enhanced Biohydrogen Production through Dark Fermentation by Humic Acid: Insights into Microbial Composition and Functional Genes.","authors":"Liguo Zhang, Yanan Bai, Jing Sang, Jinru Dong, Xiujuan Wu, Qiaoying Ban","doi":"10.4014/jmb.2412.12071","DOIUrl":"10.4014/jmb.2412.12071","url":null,"abstract":"Biohydrogen production from organic waste or wastewater by eco-friendly methods has attracted attention in recent years. However, the biohydrogen yield still far below the theoretical value. In this study, humic acid (HA), a solid redox mediator (RM), was used to enhance the biohydrogen production from glucose. The internal mechanism based on microbial community and functional genes were explored. The results showed that the optimal dosages of HA were 80 and 150 mg/l with the biohydrogen yield of 312.7 and 315.5 ml/g glucose, which was higher than that in control by above 26.6%. A similar pattern of volatile fatty acids (VFAs) could be observed in all fermentation systems. Ethanol, acetate and propionate were the dominant by-products in all fermentation systems during the biohydrogen production process. The acetate concentration was significantly improved by adding 80 mg/l of HA. Microbial composition indicated that Thermomarinilinea was the most dominant bacterial genus in the fermentation systems containing HA. Compared with control, its relative abundance was increased by 1.0-fold~3.9-fold. However, redundancy analysis (RDA) indicated biohydrogen yield was closely correlated with Gimesia, Longilinea, Defluviimonas, Pirellula and Planctomicrobium. The functional genes based on KEGG pathways showed that most biohydrogen-producing related genes had not been significantly increased in the optimal dosage of HA systems compared with that in control, indicating that biohydrogen production was enhanced by HA might depend on accelerating electron transfer and adjusting microbial community in this study.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412071"},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0