Journal of microbiology and biotechnology最新文献

Inhibition of Melanin Synthesis and Inflammation by Exosomes Derived from Leuconostoc mesenteroides DB-14 Isolated from Camellia japonica Flower.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-01-06 DOI: 10.4014/jmb.2411.11080

Byeong Min Choi, Tae-Jin Park, HuSang Harry Lee, Hyehyun Hong, Won-Jae Chi, Seung-Young Kim

{"title":"Inhibition of Melanin Synthesis and Inflammation by Exosomes Derived from Leuconostoc mesenteroides DB-14 Isolated from Camellia japonica Flower.","authors":"Byeong Min Choi, Tae-Jin Park, HuSang Harry Lee, Hyehyun Hong, Won-Jae Chi, Seung-Young Kim","doi":"10.4014/jmb.2411.11080","DOIUrl":"https://doi.org/10.4014/jmb.2411.11080","url":null,"abstract":"Leuconostoc mesenteroides is a lactic acid bacteria found in fermented products. In our previous study, L. mesenteroides was isolated from Camellia japonica flowers, and its acid tolerance and antibacterial properties were thoroughly investigated. This study focuses on the inhibition of melanin synthesis and inflammation of exosomes derived from L. mesenteroides. Moreover, L. mesenteroides exosomes (DB-14 exosome) exhibited significant inhibitory effects on inflammation and melanogenesis. At concentrations of 4.44 × 108, 8.88 × 108, and 1.78 × 109 particles/ml, the exosomes reduced nitric oxide and prostaglandin E2 activity while maintaining the growth of RAW 264.7 macrophages. In addition, proinflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha, were rarely expressed, and western blot revealed that L. mesenteroides DB-14 derived exosomes inhibited inducible nitric oxide synthase and cyclooxygenase-2 expression. Moreover, the exosomes had no toxic effects on B16F10 melanoma cells at concentrations of 1.78 × 109, 3.55 × 109, and 7.10 × 109 particles/ml, and they suppressed melanogenesis by reducing tyrosinase activity. Furthermore, western blot analysis demonstrated that microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase related protein (TRP)-1, and TRP-2 were evidently reduced, ultimately repressing melanin production. Moreover, MITF expression was inhibited by reduced mitogen-activated protein kinase and protein kinase B phosphorylation levels. Overall, this study proves the efficacy of the novel DB-14 exosome as a strong lightening and anti-inflammatory agent.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411080"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss in Pluripotency Markers in Mesenchymal Stem Cells upon Infection with Chlamydia trachomatis. 感染沙眼衣原体后间质干细胞多能性标志物的丧失

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-12-07 DOI: 10.4014/jmb.2406.06023

Munir A Al-Zeer, Mohammad Abu Lubad

{"title":"Loss in Pluripotency Markers in Mesenchymal Stem Cells upon Infection with Chlamydia trachomatis.","authors":"Munir A Al-Zeer, Mohammad Abu Lubad","doi":"10.4014/jmb.2406.06023","DOIUrl":"10.4014/jmb.2406.06023","url":null,"abstract":"The intracellular pathogen Chlamydia trachomatis can inflict substantial damage on the host. Notably, Chlamydia infection is acknowledged for its precise modulation of diverse host signaling pathways to ensure cell survival, a phenomenon intricately connected to genetic regulatory changes in host cells. To monitor shifts in gene regulation within Chlamydia-infected cells, we employed mesenchymal stem cells (MSCs) as a naïve, primary cell model. Utilizing biochemical methods and imaging, our study discloses that acute Chlamydia infection in human MSCs leads to the downregulation of transcription factors Oct4, Sox2, and Nanog, suggesting a loss of pluripotency markers. Conversely, pluripotency markers in MSCs were sustained through treatment with conditioned medium from infected MSCs. Additionally, there is an augmentation in alkaline phosphatase activity, along with elevated Sox9 and CD44 mRNA expression levels observed during acute infection. A comprehensive screening for specific cell markers using touchdown PCR indicates an upregulation of mRNA for the early chondrogenesis gene Sox9 and a decrease in mRNA for the MSC marker vimentin. Real-time PCR quantification further corroborates alterations in gene expression, encompassing increased Sox9 and CD44 mRNA levels, alongside heightened alkaline phosphatase activity. In summary, the infection of MSCs with C. trachomatis induces numerous genetic deregulations, implying a potential trend towards differentiation into chondrocytes. These findings collectively underscore a targeted impact of Chlamydia on the gene regulations of host cells, carrying significant implications for the final fate and differentiation of these cells.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2465-2473"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triple-Branch Catalytic Assembly DNAzyme Motivated DNA Tweezer for Sensitive and Reliable mecA Gene Detection in Staphylococcus aureus. 用于灵敏可靠地检测金黄色葡萄球菌中 mecA 基因的三分支催化组装 DNA 酶动机 DNA 镊子

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-01 DOI: 10.4014/jmb.2409.09008

Xiaoyang Li, Meiyan Xu, Fangmin Gan, Hui Zhao

{"title":"Triple-Branch Catalytic Assembly DNAzyme Motivated DNA Tweezer for Sensitive and Reliable mecA Gene Detection in Staphylococcus aureus.","authors":"Xiaoyang Li, Meiyan Xu, Fangmin Gan, Hui Zhao","doi":"10.4014/jmb.2409.09008","DOIUrl":"10.4014/jmb.2409.09008","url":null,"abstract":"Staphylococcus aureus (S. aureus, SA) is one of the most common bacteria in nosocomial infections. Sensitive and efficient analysis of methicillin-resistance of SA is crucial for improving the nursing performance of pneumonia. However, methicillin-resistance analysis with favorable sensitivity and specificity in an enzyme-free manner remains a huge challenge. This paper presents the development of a new fluorescent biosensor for detecting mecA gene using a triple-branch catalytic hairpin assembly (CHA) triggered DNAzyme switch-based DNA tweezer. The SA from the samples are immobilized on the plate's surface using the protein A antibody. The biosensor possesses several key features. Firstly, it utilizes dual signal amplification processes, specifically the triple-branch CHA and DNAzyme controlled DNA tweezer-based signal recycling, to enable mecA detection on the plate. This design enhances the method's sensitivity, resulting in a low limit of detection of 1.5 fM. Secondly, the biosensor does not rely on enzymes for mecA analysis, ensuring a high level of stability during target analysis. Lastly, the method demonstrates a remarkable selectivity by accurately distinguishing target sequences from non-target sequences. The proposed biosensor, which does not require enzymes and has a high level of sensitivity, offers a viable platform for the rapid and simple quantification of mecA in SA.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2450-2456"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Icariside II Preparation from Icariin Separated from Epimedium Herbal Extract Powder by a Special Icariin Glycosidase. 用一种特殊的淫羊藿苷糖苷酶从淫羊藿草药提取物粉末中分离出淫羊藿苷制备淫羊藿苷 II。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-24 DOI: 10.4014/jmb.2408.08046

Xinyu Liu, Siyu Xu, Chunying Liu, Zhenghao Wang, Bo Wu, Meijuan Guo, Changkai Sun, Hongshan Yu

{"title":"Icariside II Preparation from Icariin Separated from Epimedium Herbal Extract Powder by a Special Icariin Glycosidase.","authors":"Xinyu Liu, Siyu Xu, Chunying Liu, Zhenghao Wang, Bo Wu, Meijuan Guo, Changkai Sun, Hongshan Yu","doi":"10.4014/jmb.2408.08046","DOIUrl":"10.4014/jmb.2408.08046","url":null,"abstract":"In this study, icariside II was prepared from icariin by a special enzyme. The yield of the substrate icariin from a powdered extract of the popular herb Epimedium was 16.9%. The enzyme, which was produced from Aspergillus sp.y48 fermentation, hydrolyzes icariin to icariside II and was characterized. The molecular weight was 75 kDa, while the optimum temperature and pH were 45°C and 5.0. The purified enzyme hydrolyzed the 7-O-glucoside of icariin or epimedin A, B, and C to icariside II, or sagittatoside A, B, and C, respectively, and further hydrolyzed the terminal 3-O-xyloside of sagittatoside B to icariside II. The enzyme is a special icariin glycosidase that hydrolyzed icariin to icariside II at low cost. Based on the crude enzyme's reaction dynamics, the optimal conditions for icariside II preparation showed that 2% icariin reacted at 45°C for 6 to 9 h. Here, we obtained 13.3 g icariside II and 0.45 g of the by-product icaritin from 20 g icariin. The icariside II molar yield was 87.4%, the by-product icaritin yield was 4.1%, and the total molar yield was 91.5%. Therefore, icariside II was resoundingly prepared from an icariin glycosidase of an Epimedium extract using a non-GMO, crude enzyme from Aspergillus sp.y48. The obtained icariside II and the by-product icaritin can be directly applied in the production of cosmetics and pharmaceuticals.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2683-2692"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory Effects of Cryptotanshinone and Dihydrotanshinone I on Intracellular Trafficking of Viral Glycoproteins.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-25 DOI: 10.4014/jmb.2409.09050

Makoto Muroi, Dong-Sun Lee

{"title":"Inhibitory Effects of Cryptotanshinone and Dihydrotanshinone I on Intracellular Trafficking of Viral Glycoproteins.","authors":"Makoto Muroi, Dong-Sun Lee","doi":"10.4014/jmb.2409.09050","DOIUrl":"10.4014/jmb.2409.09050","url":null,"abstract":"Antiviral agents that target the viral envelope surface glycoproteins can disrupt the interactions between the viral glycoproteins and host cell receptors, thereby preventing viral entry into host cells. However, the mechanisms underlying glycoprotein processing and cellular trafficking have not been fully elucidated. In this study, we aimed to investigate the mechanism of action of cryptotanshinone (CTN) and dihydrotanshinone I (DTN) as inhibitors of viral glycoprotein trafficking, by assessing their inhibitory action on syncytium formation and cytopathic effects. CTN and DTN were isolated and characterized from Salvia miltiorrhiza; they effectively inhibited syncytium formation in Newcastle disease virus-infected baby hamster kidney cells. Both compounds inhibited the transport of viral G-proteins to the cell surface, resulting in intracellular accumulation. These results suggest that CTN and DTN are potential glycoprotein trafficking inhibitors that function at the Golgi apparatus. Overall, our results indicate that CTN and DTN suppress intracellular glycosylation by competing as inhibitors of glycosylation trafficking.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2457-2464"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of Codium fragile Extract against Acetaminophen-Induced Liver Injury. 脆弱钠提取物对对乙酰氨基酚引起的肝损伤的保护作用

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-25 DOI: 10.4014/jmb.2409.09061

Yea-Lim Lee, Ji-Yun Lee, Joo-Woong Park, Jin Lee, Hyun-Hoo Lee, Dae-Hee Lee

{"title":"Protective Effects of Codium fragile Extract against Acetaminophen-Induced Liver Injury.","authors":"Yea-Lim Lee, Ji-Yun Lee, Joo-Woong Park, Jin Lee, Hyun-Hoo Lee, Dae-Hee Lee","doi":"10.4014/jmb.2409.09061","DOIUrl":"10.4014/jmb.2409.09061","url":null,"abstract":"Acetaminophen (APAP) is a well-known analgesic used globally. Generally, APAP has been proven to be safe and effective at therapeutic doses; however, it can cause serious liver damage when administered at high levels. We prepared Codium fragile extract (CFE) using the seaweed C. fragile and confirmed that the CFE contains a substance called Loliolide with antioxidant activity. We performed the present study to determine whether CFE protects HEPG2 cells and BALB/c mice from oxidative stress-induced liver damage. We confirmed that CFE and Loliolide were non-cytotoxic and protected against liver damage by reducing the activities of ALT and AST, which were increased by APAP treatment, and that CFE reduced the mRNA expression of inflammatory cytokines TNF-α and IL-6 and inhibited the phosphorylation of ERK and p38 in HEPG2 cells as determined by RT-PCR and Western blot analyses. Furthermore, the TNF-α and IL-6 levels, which were increased after APAP treatment in BALB/c mice, decreased after CFE treatment. Therefore, we demonstrated that CFE exerts a protective effect against APAP-induced liver injury by suppressing the inflammatory response through anti-inflammatory activity. Our findings provide new perspectives for developing functional foods that utilize seaweeds to improve liver function.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2675-2862"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Kimchi-Derived Lactic Acid Bacteria on Reducing Biological Hazards in Kimchi. 泡菜衍生乳酸菌对减少泡菜中生物危害的影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-25 DOI: 10.4014/jmb.2408.08016

Yeonsoo Shim, Jae Yong Lee, Jihye Jung

{"title":"Effects of Kimchi-Derived Lactic Acid Bacteria on Reducing Biological Hazards in Kimchi.","authors":"Yeonsoo Shim, Jae Yong Lee, Jihye Jung","doi":"10.4014/jmb.2408.08016","DOIUrl":"10.4014/jmb.2408.08016","url":null,"abstract":"This study was performed to investigate the use of plant-based lactic acid bacteria (LAB) to reduce microbiological hazards in kimchi. Cell-free supernatants (CFS) from four LAB strains isolated from kimchi were tested for antimicrobial activity against five foodborne pathogens and two soft-rot pathogens. Each CFS showed antimicrobial activity against both foodborne and soft-rot pathogens. Washing salted kimchi cabbages inoculated with B. cereus with 5% CFS inhibited B. cereus to a greater extent than NaClO. The CFS from WiKim 83 and WiKim 87 exhibits inhibition rates of 25.09% and 24.21%, respectively, compared to the 19.19% rate of NaClO. Additionally, the CFS from WiKim 116 and WiKim 117 showed inhibition rates of 18.74% and 20.03%, respectively. Direct treatment of kimchi cabbage with soft-rot pathogens and CFS for five days inhibited the pathogens with similar efficacy to that of NaClO. To elucidate the antimicrobial activity mechanisms, pH neutralization, heat treatment, and organic acid analyses were performed. pH neutralization reduced the antimicrobial activity, whereas heat treatment did not, indicating that lactic, acetic, citric, and phenyllactic acids contribute to the thermal stability and antimicrobial properties of CFS. This study suggests that the four kimchi-derived LAB, which maintain a low pH through organic acid production, could be viable food preservatives capable of reducing biological hazards in kimchi.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2586-2595"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Succinic Acid by Metabolically Engineered Actinobacillus succinogenes from Lignocellulosic Hydrolysate Derived from Barley Straw. 代谢工程放线菌从大麦秸秆木质纤维素水解物中生产琥珀酸

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-25 DOI: 10.4014/jmb.2410.10053

Bo-Kyung Kim, Min-Seo Park, Minseok Cha, Young-Lok Cha, Soo-Jung Kim

{"title":"Production of Succinic Acid by Metabolically Engineered Actinobacillus succinogenes from Lignocellulosic Hydrolysate Derived from Barley Straw.","authors":"Bo-Kyung Kim, Min-Seo Park, Minseok Cha, Young-Lok Cha, Soo-Jung Kim","doi":"10.4014/jmb.2410.10053","DOIUrl":"10.4014/jmb.2410.10053","url":null,"abstract":"Succinic acid is an industrially important component that plays a key role in food additives, dietary supplements, and precursors for biodegradable polymers. Due to environmental and economic issues, succinic acid production has become increasingly attractive. This work aimed to improve succinic acid production from lignocellulosic biomass in Actinobacillus succinogenes through genetic modifications and fermentation strategies. Firstly, the effects on succinic acid production by overexpressing genes encoding phosphoenol carboxylase, malate dehydrogenase, and fumarase were evaluated in batch fermentations of engineered A. succinogenes strains. The engineered A. succinogenes expressing PCK, MDH, and FUM (AS-PMF) showed a 1.3-fold increase in succinic acid production compared to the wild-type strain. Subsequently, the fed-batch fermentation with MgCO3 was carried out using AS-PMF, which led to producing 50 g/l of succinic acid with 0.79 g/g of yield. Finally, 22.2 g/l of succinic acid with 0.64 g/g of yield was achieved in batch fermentation from lignocellulosic hydrolysate of barley straw. These results support that sustainable succinic acid from agricultural wastes might be a promising strategy for industrial applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2618-2626"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shifts in Soil Bacterial Community Composition of Jujube Orchard Influenced by Organic Fertilizer Amendment.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-30 DOI: 10.4014/jmb.2406.06037

Heesoon Park, Kiyoon Kim, Denver I Walitang, Riyaz Sayyed, Tongmin Sa

{"title":"Shifts in Soil Bacterial Community Composition of Jujube Orchard Influenced by Organic Fertilizer Amendment.","authors":"Heesoon Park, Kiyoon Kim, Denver I Walitang, Riyaz Sayyed, Tongmin Sa","doi":"10.4014/jmb.2406.06037","DOIUrl":"10.4014/jmb.2406.06037","url":null,"abstract":"Organic fertilizer application in agricultural land is known to improve soil microbial processes, fertility, and yield. In particular, the changes in soil chemical composition due to multi-year application of organic fertilizers are thought to alter the microbial community. Here, the effects of organic fertilization with oil-cake amendments (OC) on soil bacterial diversity, community profile, and enzyme activity were evaluated and compared to those amended with chemical fertilizer (NPK). Diversity indices show that the application of organic fertilizer potentially increases microbial diversity as well as the number of different microbial groups. The ordination plot distinguished and clustered both treatments, showing the differential effects of soil chemical factors on the microbial communities in each treatment. Proteobacteria, Verrucomicrobia, and Bacteriodetes were significantly more abundant in OC-amended soil than in the NPK soil, indicating alterations in community structure, composition, and diversity, concurrent to the changes in the pH, Ca, and Mg contents of the soil. These shifts in bacterial community structure and composition, partially explained by differences in soil chemical factors, could be observed from the phylum to the genus level in NPK- and OC-amended soils. The OC soil contained a significantly higher abundance of predicted genes corresponding to enzymes related to biogeochemical cycling, decomposition, and plant growth promotion. Collectively, these results support the use of an unconventional organic fertilizer positively altering bacterial populations in jujube orchards. The application of an unconventional organic fertilizer improved microbial diversity and enhanced ecosystem functions related to biogeochemical cycles, mineralization, and plant growth promotion.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2539-2546"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shiga Toxins Produced by Enterohaemorrhagic Escherichia coli Induce Inflammation in Toxin-Sensitive Cells through the p38 MAPK/MK2/Tristetraprolin Signaling Pathway.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-22 DOI: 10.4014/jmb.2410.10016

Seo Young Park, Yu-Jin Jeong, Kyung-Soo Lee, Jun-Young Park, Jongsun Park, Vernon L Tesh, Moo-Seung Lee

{"title":"Shiga Toxins Produced by Enterohaemorrhagic Escherichia coli Induce Inflammation in Toxin-Sensitive Cells through the p38 MAPK/MK2/Tristetraprolin Signaling Pathway.","authors":"Seo Young Park, Yu-Jin Jeong, Kyung-Soo Lee, Jun-Young Park, Jongsun Park, Vernon L Tesh, Moo-Seung Lee","doi":"10.4014/jmb.2410.10016","DOIUrl":"10.4014/jmb.2410.10016","url":null,"abstract":"Shiga toxins (Stxs), produced by Shigella dysenteriae serotype 1 and certain Escherichia coli pathotypes, cause hemorrhagic colitis, which can progress to hemolytic uremic syndrome (HUS) and central nervous system (CNS) pathology. The underlying mechanisms of toxin-induced inflammation remain unclear. The p38 mitogen-activated protein kinase (MAPK) and its downstream target, MAPKAPK2 (MK2), play key roles in various cellular responses. We identified Tristetraprolin (TTP) as a novel substrate of MK2 in Stx-intoxicated cells. Western blot analysis showed that Stxs induce phosphorylation of MK2 (Thr334) and TTP in globotriaosylceramide (Gb3)-positive cells, including D-THP-1 macrophage-like cells and HK-2 renal epithelial cells, but not in Gb3-negative T84 colon carcinoma cells. After treatment with wild-type Stx, the activity of phosphorylated MK2 and TTP persists for up to 8 h, while Stx2amut, which lacks N-glycosidase activity, causes transient MK2/TTP phosphorylation. This suggests that Stxs selectively mediate MK2 and TTP activation in a Gb3-dependent manner. Knockdown of TTP in Stx2a-treated D-THP-1 cells upregulates proinflammatory cytokines such as TNF-α, IL-1β, IL-6, IL-8, MCP-1, and MIP-1α. The MK2 inhibitor PF-3644022 significantly reduces TTP phosphorylation and blocks the production of IL-6, IL-8, MCP-1, and MIP-1α in Stx2a-stimulated HK-2 cells. In conclusion, the MK2-TTP signaling pathway regulates the inflammatory response induced by Stxs in toxin-sensitive cells.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"2439-2449"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0