Journal of microbiology and biotechnology最新文献

Human Placenta Extract (HPH) Suppresses Inflammatory Responses in TNF-α/IFN-γ-Stimulated HaCaT Cells and a DNCB Atopic Dermatitis (AD)-Like Mouse Model. 人胎盘提取物（HPH）可抑制TNF-α/IFN-γ刺激的HaCaT细胞和DNCB特应性皮炎（AD）样小鼠模型的炎症反应。

IF 2.8 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-11 DOI: 10.4014/jmb.2406.06045

Jung Ok Lee,Youna Jang,A Yeon Park,Jung Min Lee,Kyeongsoo Jeong,So-Hyun Jeon,Hui Jin,Minju Im,Jae-Won Kim,Beom Joon Kim

{"title":"Human Placenta Extract (HPH) Suppresses Inflammatory Responses in TNF-α/IFN-γ-Stimulated HaCaT Cells and a DNCB Atopic Dermatitis (AD)-Like Mouse Model.","authors":"Jung Ok Lee,Youna Jang,A Yeon Park,Jung Min Lee,Kyeongsoo Jeong,So-Hyun Jeon,Hui Jin,Minju Im,Jae-Won Kim,Beom Joon Kim","doi":"10.4014/jmb.2406.06045","DOIUrl":"https://doi.org/10.4014/jmb.2406.06045","url":null,"abstract":"Atopic dermatitis (AD), a chronic inflammatory disease, severely interferes with patient life. Human placenta extract (HPH; also known as human placenta hydrolysate) is a rich source of various bioactive substances and has widely been used to dampen inflammation, improve fatigue, exert anti-aging effects, and promote wound healing. However, information regarding HPH's incorporation in AD therapies is limited. Therefore, this study aimed to evaluate HPH's effective potential in treating AD using tumor necrosis factor (TNF)-α/interferon (IFN)-γ-stimulated human keratinocytes (HaCaT), immunized splenocytes, and a 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model. In TNF-α /IFN-γ-stimulated HaCaT cells, HPH markedly reduced the production of reactive oxygen species (ROS) and restored the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), superoxide dismutase 1(SOD1), catalase, and filaggrin (FLG). HPH reduced interleukin (IL)-6; thymus- and activation-regulated chemokine (TARC); thymic stromal lymphopoietin (TSLP); and regulated upon activation, normal T cell expressed and presumably secreted (RANTES) levels and inhibited nuclear factor kappa B phosphorylation. Additionally, HPH suppressed the T helper 2 (Th2) immune response in immunized splenocytes. In the AD-like mouse model, it significantly mitigated the DNCB-induced elevation in infiltrating mast cells and macrophages, epidermal thickness, and AD symptoms. HPH also reduced TSLP levels and prevented FLG downregulation. Furthermore, it decreased the expression levels of IL-4, IL-5, IL-13, TARC, RANTES, and immunoglobulin E (IgE) in serum and AD-like skin lesion. Overall, our findings demonstrate that HPH effectively inhibits AD development and is a potentially useful therapeutic agent for AD-like skin disease.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Community of Breast Milk in Breastfeeding Women Using CultureDependent and Culture-Independent Approaches. 用培养依赖型和培养独立型方法分析哺乳期妇女母乳中的细菌群落。

IF 2.8 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-09 DOI: 10.4014/jmb.2407.07001

Sumin Lee,Sojeong Heo,Mi-Kyung Park,Moon-Hee Sung,Do-Won Jeong

{"title":"Bacterial Community of Breast Milk in Breastfeeding Women Using CultureDependent and Culture-Independent Approaches.","authors":"Sumin Lee,Sojeong Heo,Mi-Kyung Park,Moon-Hee Sung,Do-Won Jeong","doi":"10.4014/jmb.2407.07001","DOIUrl":"https://doi.org/10.4014/jmb.2407.07001","url":null,"abstract":"This study aimed to analyze bacterial communities in breast milk obtained from five breastfeeding women. Culture-dependent and culture-independent methods were used to analyze microbial communities. Total bacterial count of breast milk determined using plate count agar ranged from 3.3 × 104 ± 3.5 × 102 colony forming unit (CFU)/g to 1.7 × 105 ± 3.5 × 103 CFU/g, with a pH between 6.4 and 6.8. Only three species, Leuconostoc citreum (17 out of 160 strains; 10.63%), Staphylococcus epidermidis (118 strains; 73.75%), and Staphylococcus lugdunensis (25 strains; 15.63%), belong to the phylum Bacillota were detected by culture-dependent analysis. Microbial communities analyzed via pyrosequencing revealed greater diversity compared to the culture-dependent analysis. At the phylum level, Bacillota accounted for 60.9% of the microbial community. At the genus level, Staphylococcus (24.57%), Streptococcus (22.93%), and Methylobacterium (8.76%) were dominant genera. While pyrosequencing demonstrated greater microbial diversity than the agar plate culture method, identified microbes might lack information or include many unculturable microbes. Most of all, considering the low total bacterial count averaging 7.2 × 104 CFU/g, further research is needed to determine the significance of microbial presence in breast milk.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural Inhibitory Treatment of FungiInduced Deterioration of Carbonate and Cellulosic Ancient Monuments: Isolation, Identification and Simulation of Biogenic Deterioration. 对真菌引起的碳酸盐和纤维素古迹劣化的自然抑制处理：生物劣化的分离、鉴定和模拟。

IF 2.8 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-30 DOI: 10.4014/jmb.2404.04032

Mohamed H El-Sayed,Maha AlHarbi,Islam A Elsehemy,Wafaa M Haggag,Bahgat M Refaat,Sharaf M Ali,Amr Elkelish

{"title":"Natural Inhibitory Treatment of FungiInduced Deterioration of Carbonate and Cellulosic Ancient Monuments: Isolation, Identification and Simulation of Biogenic Deterioration.","authors":"Mohamed H El-Sayed,Maha AlHarbi,Islam A Elsehemy,Wafaa M Haggag,Bahgat M Refaat,Sharaf M Ali,Amr Elkelish","doi":"10.4014/jmb.2404.04032","DOIUrl":"https://doi.org/10.4014/jmb.2404.04032","url":null,"abstract":"Fungi play a significant role in the deterioration of various types of monuments. Therefore, the protection of ancient monuments from fungal attacks is an important goal that must attract the attention of researchers worldwide. A total of 69 fungal isolates were recovered from 22 deteriorated objects compromising paper, textiles, wood, and stone in the National Museum of Egyptian Civilization (NMEC) storeroom, Cairo, Egypt. The isolates were identified as 12 different species categorized into three different genera, namely, Aspergillus (9 species), Penicillium (2 species) and Trichoderma (1 species). Among them, Aspergillus fumigatus was the most prevalent species. Three essential oils were assessed for antifungal activity and compared with the antifungal effects of five synthetic microcides to identify a natural inhibitory treatment. Thyme oil and sodium azide were found to be the most active growth inhibitors, with minimum inhibitory concentrations (MICs) of 625 and 100 ppm, with inhibition zone diameters of 19.0 ± 0.70 - 23.76 ± 1.15 and 13.30 ± 0.35 - 19.66 ± 0.54 mm, respectively. An in vitro simulation of the biodeterioration process was conducted using spores of the A. fumigatus strain NMEC-PSTW.1 on model cubes made of paper, textile, wood, and stone materials. The changes in the characteristics of the artificially deteriorated materials were analyzed using environmental scanning electron microscopy/energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy. The results revealed changes in the morphology, physical properties, and chemical composition induced by A. fumigatus NMEC-PSTW.1. Overall, thyme oil is recommended as a natural inhibitor to protect carbonate and cellulosic monuments in NMEC against fungal attack.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Genomic Analyses of E. coli ST2178 Strains Originated from Wild Birds in Pakistan. 巴基斯坦野生鸟类大肠杆菌 ST2178 菌株基因组比较分析

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-29 DOI: 10.4014/jmb.2407.07026

Jung Hun Lee, Abdul Rauf Tareen, Nam-Hoon Kim, Chanyeong Jeong, Byeonghyeon Kang, Gwangje Lee, Dae-Wi Kim, Rabaab Zahra, Sang Hee Lee

{"title":"Comparative Genomic Analyses of E. coli ST2178 Strains Originated from Wild Birds in Pakistan.","authors":"Jung Hun Lee, Abdul Rauf Tareen, Nam-Hoon Kim, Chanyeong Jeong, Byeonghyeon Kang, Gwangje Lee, Dae-Wi Kim, Rabaab Zahra, Sang Hee Lee","doi":"10.4014/jmb.2407.07026","DOIUrl":"https://doi.org/10.4014/jmb.2407.07026","url":null,"abstract":"The emergence and spread of multidrug-resistance (MDR) pathogenic Escherichia coli due to horizontal gene transfer of antibiotic resistance genes (ARGs) and virulence factors (VFs) is a global health concern, particularly in developing countries. While numerous studies have focused on major sequence types (STs), the implication of minor STs in ARG dissemination and their pathogenicity remains crucial. In this study, two E. coli strains (PEC1011 and PEC1012) were isolated from wild bird feces in Pakistan and identified as ST2178 based on their complete genome sequences. To understand this minor ST, 204 genome assemblies of ST2178 were comparatively analyzed with the isolates' genomes. The phylogenetic analyses revealed five subclades of ST2178. Subclade E strains were predominantly isolated from human specimens, whereas subclades A and B strains including strains PEC1011 and PEC1012, respectively, were frequently isolated from animal. Mobile genetic elements (MGEs) exhibited the positive correlation with ARGs but not with VFs in this ST. Plasmid-borne ARGs exhibited higher correlation with plasmid-borne MGEs, indicating the role of diverse mobile plasmid structures in ARG transmission. Subclade E exhibited diverse plasmid-borne ARG repertoires correlated with MGEs, marking it as a critical surveillance target. In the case of VFs, they exhibited phylogeny-dependent profiles. Strain PEC1012 harbored various plasmid-borne ARGs, which are similar with conserved ARG repertoires in subclade A. The presence of unique ARG insertion in pPEC1012 highlights the importance of subclade A in ARG dissemination. This study comprehensively elucidates the landscape of ST2178, identifying critical phylogenetic subclades and their characteristics in ARG and VF occurrence.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paenibacillus gyeongsangnamensis sp. nov., Isolated from Soil. Paenibacillus gyeongsangnamensis sp.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-17 DOI: 10.4014/jmb.2404.04038

Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim

{"title":"Paenibacillus gyeongsangnamensis sp. nov., Isolated from Soil.","authors":"Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim","doi":"10.4014/jmb.2404.04038","DOIUrl":"10.4014/jmb.2404.04038","url":null,"abstract":"A Gram-stain-positive, aerobic, white-coloured, rod-shaped bacteria, designated as a strain dW9T, was isolated from soil. Strain dW9T was catalase-positive and oxidase-negative. Strain dW9T grew at temperature of 20-37°C and at pH of 5.0-7.0. Phylogenetic and 16S rRNA gene analysis indicated that strain dW9T belonged to the genus Paenibacillus with its closest relative being Paenibacillus filicis S4T (97.4% sequence similarity). The genome size of dW9T was 7,787,916 bp with DNA G+C content of 51.3%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values of dW9T with its closest relatives were found to be <22.0% and <74.0%, respectively. The only respiratory quinone was MK-7, and the major fatty acids were antiso-C15:0 and iso-C16:0. Overall, the comprehensive taxonomic analysis revealed that strain dW9T met all the fundamental criteria to be classified as a novel species within the genus Paenibacillus. Accordingly, we propose the name Paenibacillus gyeongsangnamensis sp. nov., with the type strain dW9T (=KCTC 43431T =NBRC 116022T).","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Menopausal Effect of Heat-Killed Bifidobacterium breve HDB7040 via Estrogen Receptor-Selective Modulation in MCF-7 Cells and Ovariectomized Rats. 在 MCF-7 细胞和卵巢切除大鼠体内通过雌激素受体（ER）选择性调节作用抗绝经的热杀灭双歧杆菌 HDB7040

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-17 DOI: 10.4014/jmb.2402.02035

Hyeon Jeong Kim, Kyung Min Kim, Min-Kyu Yun, Duseong Kim, Johann Sohn, Ji-Won Song, Seunghun Lee

{"title":"Anti-Menopausal Effect of Heat-Killed Bifidobacterium breve HDB7040 via Estrogen Receptor-Selective Modulation in MCF-7 Cells and Ovariectomized Rats.","authors":"Hyeon Jeong Kim, Kyung Min Kim, Min-Kyu Yun, Duseong Kim, Johann Sohn, Ji-Won Song, Seunghun Lee","doi":"10.4014/jmb.2402.02035","DOIUrl":"10.4014/jmb.2402.02035","url":null,"abstract":"Menopause is induced by spontaneous ovarian failure and leads to life quality deterioration with various irritating symptoms. Hormonal treatment can alleviate these symptoms, but long-term treatment is closely associated with breast and uterine cancer, and stroke. Therefore, developing alternative therapies with novel anti-menopausal substances and improved safety is needed. In our study, heat-killed Bifidobacterium breve HDB7040 significantly promoted MCF-7 cell proliferation in a dose-dependent manner under estrogen-free conditions, similar to 17β-estradiol. This strain also triggered ESR2 expression, but not ESR1, in MCF-7 cells. Moreover, administrating HDB7040 to ovariectomized (OVX) Sprague-Dawley (SD) female rats reduced estrogen deficiency-induced weight gain, fat mass, blood triglyceride, and total cholesterol levels. It also recovered collapsed trabecular microstructure by improving trabecular morphometric parameters (bone mineral density, bone volume per tissue volume, trabecular number, and trabecular separation) and decreasing blood alkaline phosphatase levels with no significant changes in uterine size and blood estradiol. HDB7040 also significantly regulated the expression of Tff1, Pgr, and Esr2, but not Esr1 in uteri of OVX rats. Heat-killed B. breve HDB7040 exerts an anti-menopausal effect via the specific regulation of ERβ in vitro and in vivo, suggesting its potential as a novel substance for improving and treating menopausal syndrome.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus siamensis 3BS12-4 Extracellular Compounds as a Potential Biological Control Agent against Aspergillus flavus. 将暹罗芽孢杆菌 3BS12-4 胞外化合物作为潜在的黄曲霉生物控制剂。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-30 DOI: 10.4014/jmb.2402.02053

Patapee Aphaiso, Polson Mahakhan, Jutaporn Sawaengkaew

{"title":"Bacillus siamensis 3BS12-4 Extracellular Compounds as a Potential Biological Control Agent against Aspergillus flavus.","authors":"Patapee Aphaiso, Polson Mahakhan, Jutaporn Sawaengkaew","doi":"10.4014/jmb.2402.02053","DOIUrl":"10.4014/jmb.2402.02053","url":null,"abstract":"Aspergillus flavus, the primary mold that causes food spoilage, poses significant health and economic problems worldwide. Eliminating A. flavus growth is essential to ensure the safety of agricultural products, and extracellular compounds (ECCs) produced by Bacillus spp. have been demonstrated to inhibit the growth of this pathogen. In this study, we aimed to identify microorganisms efficient at inhibiting A. flavus growth and degrading aflatoxin B1. We isolated microorganisms from soil samples using a culture medium containing coumarin (CM medium) as the sole carbon source. Of the 498 isolates grown on CM medium, only 132 bacterial strains were capable of inhibiting A. flavus growth. Isolate 3BS12-4, identified as Bacillus siamensis, exhibited the highest antifungal activity with an inhibition ratio of 43.10%, and was therefore selected for further studies. The inhibition of A. flavus by isolate 3BS12-4 was predominantly attributed to ECCs, with a minimum inhibitory concentration and minimum fungicidal concentration of 0.512 g/ml. SEM analysis revealed that the ECCs disrupted the mycelium of A. flavus. The hydrolytic enzyme activity of the ECCs was assessed by protease, β-1,3-glucanase, and chitinase activity. Our results demonstrate a remarkable 96.11% aflatoxin B1 degradation mediated by ECCs produced by isolate 3BS12-4. Furthermore, treatment with these compounds resulted in a significant 97.93% inhibition of A. flavus growth on peanut seeds. These findings collectively present B. siamensis 3BS12-4 as a promising tool for developing environmentally friendly products to manage aflatoxin-producing fungi and contribute to the enhancement of agricultural product safety and food security.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Microplastic Exposure against White Spot Syndrome Virus Infection in Pacific White Shrimp (Penaeus vannamei). 微塑料暴露对太平洋白对虾（Penaeus vannamei）白斑综合征病毒感染的影响。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-03-22 DOI: 10.4014/jmb.2402.02001

Hye Jin Jeon, Sangsu Seo, Chorong Lee, Bumkeun Kim, Patharapol Piamsomboon, Ji Hyung Kim, Jee Eun Han

{"title":"Effects of Microplastic Exposure against White Spot Syndrome Virus Infection in Pacific White Shrimp (Penaeus vannamei).","authors":"Hye Jin Jeon, Sangsu Seo, Chorong Lee, Bumkeun Kim, Patharapol Piamsomboon, Ji Hyung Kim, Jee Eun Han","doi":"10.4014/jmb.2402.02001","DOIUrl":"10.4014/jmb.2402.02001","url":null,"abstract":"Plastic waste has emerged as a major environmental concern in recent years. As plastic waste discharged into the marine environment, it undergoes a breakdown process, eventually accumulating in aquatic organisms in the form of microplastics (MPs). To date, reduced food intake, nutritional absorption, and impaired immune system are known adverse effects of MPs-exposed aquatic organisms. This study aims to investigate whether MP exposure accelerated white spot syndrome virus (WSSV) infection in Pacific white shrimp (Penaeus vannamei) via laboratory tests. Briefly, experimental shrimp were divided into four groups; WSSV (group 1); MP (group 2); WSSV + MP (group 3); and Control (group 4). No mortality was observed in group 2, group 4, and even in group 1. However, group 3 showed a cumulative mortality of 50% during the experimental period. The PCR assay results showed no WSSV in the other three groups (groups 1, 2, and 4), but the dead and alive shrimp collected from group 3 were confirmed to be infected with the virus. Histopathological examination revealed normal structures in the hepatopancreas, gill, and muscle tissues of group 4, whereas numerous abnormally shaped nuclei were detected in the gill tissue of group 2. Moreover, group 1 showed minor WSSV-related lesions with few basophilic inclusion bodies in the gills, interestingly, group 3 exhibited severe lesions with numerous basophilic inclusion bodies in the gills. In conclusion, this study confirmed the correlation between the viral disease of shrimp and MPs, which can cause significant economic losses to the shrimp aquaculture industry.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strongylocentrotus intermedius Extract Suppresses Adiposity by Inhibiting Adipogenesis and Promoting Adipocyte Browning via AMPK Activation in 3T3-L1 Cells. 通过激活 3T3-L1 细胞中的 AMPK 抑制脂肪生成并促进脂肪细胞褐变，从而抑制脂肪变性

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-07-12 DOI: 10.4014/jmb.2404.04041

Lakshi A Dayarathne, Jasmadi, Seok-Chun Ko, Mi-Jin Yim, Jeong Min Lee, Ji-Yul Kim, Gun-Woo Oh, Dae-Sung Lee, Won-Kyo Jung, Sei-Jung Lee, Jae-Young Je

{"title":"Strongylocentrotus intermedius Extract Suppresses Adiposity by Inhibiting Adipogenesis and Promoting Adipocyte Browning via AMPK Activation in 3T3-L1 Cells.","authors":"Lakshi A Dayarathne, Jasmadi, Seok-Chun Ko, Mi-Jin Yim, Jeong Min Lee, Ji-Yul Kim, Gun-Woo Oh, Dae-Sung Lee, Won-Kyo Jung, Sei-Jung Lee, Jae-Young Je","doi":"10.4014/jmb.2404.04041","DOIUrl":"10.4014/jmb.2404.04041","url":null,"abstract":"The current study aimed to determine whether Strongylocentrotus intermedius (S. intermedius) extract (SIE) exerts anti-obesity potentials employing 3T3-L1 cells as in vitro model. Herein we reported that treatment of SIE for 6 days reduced lipid accretion and triglyceride content whereas it increased the release of free glycerol. The inhibited lipid accumulation and induced lipolysis were evidenced by the downregulation of lipogenesis proteins, such as fatty acid synthase and lipoprotein lipase, and the upregulation of hormone-sensitive lipase expression. Furthermore, the downregulation of adipogenic transcription factors, including peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein 1, highlights that reduced lipid accumulation is supported by lowering adipocyte differentiation. Additionally, treatment activates brown adipocyte phenotype in 3T3-L1 cells by inducing expression of brown adipose tissue-specific proteins, such as uncoupling protein 1 and peroxisome proliferator-activated receptor-γ coactivator 1α. Moreover, SIE induced the phosphorylation of AMP-activated protein kinase (AMPK). The pharmacological approach using AMPK inhibitor revealed that the restraining effect of SIE on adipogenesis and promotion of adipocyte browning were blocked. In GC-MS analysis, SIE was mainly composed of cholest-5-en-3-ol (36.71%) along with saturated and unsaturated fatty acids which have favorable anti-obesity potentials. These results reveal that SIE has the possibility as a lipid-lowering agent for the intervention of obesity.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antagonistic Efficacy of Symbiotic Bacterium Xenorhabdus sp. SCG against Meloidogyne spp. 共生细菌 Xenorhabdus sp. SCG 对 Meloidogyne spp.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-07-15 DOI: 10.4014/jmb.2404.04003

Jong-Hoon Kim, Byeong-Min Lee, Hyung Chul Lee, In-Soo Choi, Kyung-Bon Koo, Kwang-Hee Son

{"title":"Antagonistic Efficacy of Symbiotic Bacterium Xenorhabdus sp. SCG against Meloidogyne spp.","authors":"Jong-Hoon Kim, Byeong-Min Lee, Hyung Chul Lee, In-Soo Choi, Kyung-Bon Koo, Kwang-Hee Son","doi":"10.4014/jmb.2404.04003","DOIUrl":"10.4014/jmb.2404.04003","url":null,"abstract":"The inhabitation and parasitism of root-knot nematodes (RKNs) can be difficult to control, as its symptoms can be easily confused with other plant diseases; hence, identifying and controlling the occurrence of RKNs in plants remains an ongoing challenge. Moreover, there are only a few biological agents for controlling these harmful nematodes. In this study, Xenorhabdus sp. SCG isolated from entomopathogenic nematodes of genus Steinernema was evaluated for nematicidal effects under in vitro and greenhouse conditions. The cell-free filtrates of strain SCG showed nematicidal activity against Meloidogyne species J2s, with mortalities of > 88% at a final concentration of 10%, as well as significant nematicidal activity against the three other genera of plant-parasitic nematodes in a dose-dependent manner. Thymine was isolated as active compounds by assay-guided fractionation and showed high nematicidal activity against M. incognita. Greenhouse experiments suggested that cell-free filtrates of strain SCG efficiently controlled the nematode population in M. incognita-infested tomatoes (Solanum lycopersicum L., cv. Rutgers). In addition, a significant increase in host plant growth was observed after 45 days of treatment. To our knowledge, this is the first to demonstrate the nematicidal activity spectrum of isolated Xenorhabdus species and their application to S. lycopersicum L., cv. Rutgers under greenhouse conditions. Xenorhabdus sp. SCG could be a promising biological nematicidal agent with plant growth-enhancing properties.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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