Journal of microbiology and biotechnology最新文献_第10页

FMT from IBS-D Rats Impairs Intestinal Barrier Function and is Associated with Increased Intestinal Benzoic Acid and Ruminococcus Abundance. IBS-D大鼠的FMT损害肠道屏障功能，并与肠道苯甲酸和瘤胃球菌丰度增加有关。

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-04 DOI: 10.4014/jmb.2602.02004

Xiangyu Xie, Weixin Yan, Xinting Zhang, Chunli Gan, Xiaochun Lei, Yusheng Huang, Wei Xiong, Hongmei Tang, He Zhu

{"title":"FMT from IBS-D Rats Impairs Intestinal Barrier Function and is Associated with Increased Intestinal Benzoic Acid and Ruminococcus Abundance.","authors":"Xiangyu Xie, Weixin Yan, Xinting Zhang, Chunli Gan, Xiaochun Lei, Yusheng Huang, Wei Xiong, Hongmei Tang, He Zhu","doi":"10.4014/jmb.2602.02004","DOIUrl":"10.4014/jmb.2602.02004","url":null,"abstract":"While the etiology of diarrhea-predominant irritable bowel syndrome (IBS-D) is multifactorial, current studies have converged to identify gut microbiota dysbiosis as a principal contributor. This study aimed to elucidate the role of the gut microbiota in IBS-D progression and to uncover the underlying mechanisms. In this study, a rat model of IBS-D was successfully established, characterized by prominent visceral hypersensitivity and diarrhea. To assess the impact of the gut microbiota, recipient rats pretreated with broad-spectrum antibiotics underwent fecal microbiota transplantation (FMT) from IBS-D model rats. The model-microbiota recipient rats (the MR group) developed IBS-D-like symptoms, such as abdominal pain, diarrhea, and depression-like behaviors. Both the IBS-D and MR groups exhibited elevated serum diamine oxidase (DAO) concentrations, reduced intestinal tight junction protein levels, increased serum TNF-α concentrations, upregulated TNF-α mRNA expression, and downregulated IL-10 mRNA expression in the intestine. These findings indicated that the transplanted microbiota disrupted intestinal barrier integrity and triggered low-grade inflammation. Moreover, an elevated abundance of Ruminococcaceae in the gut microbiota was a common feature of both the IBS-D and MR groups. Metabolomic analysis revealed an enrichment in the phenylalanine, tyrosine, and tryptophan biosynthesis pathway in both IBS-D and recipient groups, with benzoic acid being particularly prominent. Pearson correlation analysis demonstrated a strong positive correlation between the Ruminococcus abundance and benzoic acid levels. Together, these findings indicated that FMT robustly recapitulated core IBS-D pathophysiology in recipient rats, and the identified bacteria and metabolites provided novel insights into the pathogenesis of IBS-D.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2602004"},"PeriodicalIF":3.4,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13275257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148264656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactiplantibacillus plantarum HY7715 Attenuates Oxidative Stress-Induced Neurobiological Aging-Related Changes by Modulating Senescence-Associated Markers and Gut Microbiota. 植物乳杆菌HY7715通过调节衰老相关标志物和肠道微生物群减轻氧化应激诱导的神经生物学衰老相关变化

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-02 DOI: 10.4014/jmb.2604.04063

Daehyeop Lee, Haeryn Jeong, Hyeonjun Gwon, Kippeum Lee, Joo-Yun Kim, Jae-Jung Shim, Jae-Hwan Lee

{"title":"Lactiplantibacillus plantarum HY7715 Attenuates Oxidative Stress-Induced Neurobiological Aging-Related Changes by Modulating Senescence-Associated Markers and Gut Microbiota.","authors":"Daehyeop Lee, Haeryn Jeong, Hyeonjun Gwon, Kippeum Lee, Joo-Yun Kim, Jae-Jung Shim, Jae-Hwan Lee","doi":"10.4014/jmb.2604.04063","DOIUrl":"10.4014/jmb.2604.04063","url":null,"abstract":"External stressors can accelerate biological aging-related processes by promoting oxidative stress and senescence-associated molecular alterations in the brain. This study investigated the potential of Lactiplantibacillus plantarum HY7715 to attenuate oxidative stress-induced neurobiological aging-related changes using H2O2-induced HT22 hippocampal cells and a restraint-stressed mouse model. In HT22 cells, HY7715 reduced reactive oxygen species accumulation, decreased 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels, and lowered the proportion of senescence-associated β-galactosidase-positive cells. These effects were accompanied by suppression of p53/p21 signaling and restoration of Tert expression. In restraint-stressed mice, HY7715 reduced the number of p21-positive cells in the hippocampus, significantly lowered p53 expression, restored Tert expression, reduced Il-6 expression, and improved antioxidant-related gene expression, including Gpx1 and Sod1. Microbiome analysis showed that HY7715 reshaped the stress-altered gut microbiota toward a Lactobacillus-enriched profile and reduced the abundance of Lachnospiraceae, Acetatifactor, Desulfovibrio, and Oscillibacter. Collectively, these findings suggest that HY7715 may attenuate oxidative stress-induced neurobiological aging-related changes by modulating senescence-associated molecular markers and stress-altered gut microbiota, highlighting its potential as a candidate for supporting healthy brain aging.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604063"},"PeriodicalIF":3.4,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13275255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148264559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Anti-Viral Compounds from Toxicodendron vernicifluum Extract That Inhibit the Coronavirus Replication. 抑制冠状病毒复制的毒竹提取物抗病毒化合物的鉴定。

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-01 DOI: 10.4014/jmb.2604.04038

Jihun Choi, Jang Hoon Kim, Siyun Lee, Chunghyeon Lee, Seungju Cho, Sumin Kim, Eunjin Cho, Jayhyun Park, Yu Jin Kim, Ik Soo Lee, Junsoo Park

{"title":"Identification of Anti-Viral Compounds from Toxicodendron vernicifluum Extract That Inhibit the Coronavirus Replication.","authors":"Jihun Choi, Jang Hoon Kim, Siyun Lee, Chunghyeon Lee, Seungju Cho, Sumin Kim, Eunjin Cho, Jayhyun Park, Yu Jin Kim, Ik Soo Lee, Junsoo Park","doi":"10.4014/jmb.2604.04038","DOIUrl":"10.4014/jmb.2604.04038","url":null,"abstract":"Coronavirus can cause diseases ranging from mild cough to severe COVID-19. Although vaccines and antiviral agents for coronaviruses are available, alternative strategies are still needed to address emerging variants in the future. Toxicodendron vernicifluum is known to be rich in flavonoids, which exhibit antioxidant, anti-inflammatory, anticancer, and antiviral activities. However, the antiviral activity of T. vernicifluum against coronaviruses has not been fully investigated. Here, we report that the T. vernicifluum ethanol extract (TVE) shows antiviral effects against human coronavirus. TVE treatment inhibited coronavirus replication as well as viral infectivity. To elucidate the active constituents responsible for this activity, comprehensive phytochemical profiling of TVE was performed using UPLC-Q-Orbitrap-MS, leading to the tentative identification of major phytochemicals. Based on this analytical profiling, individual compounds were isolated from TVE and evaluated for antiviral activity against coronavirus. We found that ethyl gallate, fisetin, butin, sulfuretin, and gallic acid exhibit antiviral activity against coronavirus.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604038"},"PeriodicalIF":3.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13253540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full-Length 16S rRNA Amplicon Sequencing for the Simple and Simultaneous Detection of Multiple Probiotic Species in Commercial Products. 全长16S rRNA扩增子测序用于商业产品中多种益生菌的简单和同时检测。

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-01 DOI: 10.4014/jmb.2603.03005

Gyeong-Eun Kim, Yoon-Soo Gwak, Chan-Il Bae, Kwangcheol C Jeong, Mi-Ju Kim

{"title":"Full-Length 16S rRNA Amplicon Sequencing for the Simple and Simultaneous Detection of Multiple Probiotic Species in Commercial Products.","authors":"Gyeong-Eun Kim, Yoon-Soo Gwak, Chan-Il Bae, Kwangcheol C Jeong, Mi-Ju Kim","doi":"10.4014/jmb.2603.03005","DOIUrl":"10.4014/jmb.2603.03005","url":null,"abstract":"To ensure efficacy and trust, probiotic strains in commercial products should be accurately identified. However, studies have consistently reported discrepancies between labeling and actual microbial composition. Therefore, we developed a full-length 16S rRNA amplicon sequencing workflow for species-level identification of probiotics. To optimize this workflow, four mock communities were analyzed using multiple pipelines and parameters; the workflow accuracy was evaluated. Optimal performance was achieved using Q10 filtering, the Emu pipeline with its default database, and a 0.1% relative abundance threshold. Subsequently, the workflow was applied to eight probiotic products, approximately 50% of which revealed labeling discrepancies with some labeled strains undetected. Furthermore, these results were compared and confirmed using PCR analysis. Despite some discrepancies, both approaches demonstrated overall consistency. Overall, the optimized sequencing workflow facilitates comprehensive species-level identification of probiotics within a single assay, providing a novel approach for quality control and labeling verification.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2603005"},"PeriodicalIF":3.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13246294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148157001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leonurine-Standardized Leonurus japonicus Extract Promotes Recovery from Immobilization-Induced Muscle Atrophy via PI3K/Akt/mTOR Signaling in Mice. 益母草-标准益母草提取物通过PI3K/Akt/mTOR信号通路促进小鼠固定运动诱导的肌肉萎缩的恢复

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-01 DOI: 10.4014/jmb.2604.04033

Jiyeon Lee, Mi-Bo Kim, Changhee Kim, Chae Young Moon, Hyunju Kang, Jae-Kwan Hwang

{"title":"Leonurine-Standardized Leonurus japonicus Extract Promotes Recovery from Immobilization-Induced Muscle Atrophy via PI3K/Akt/mTOR Signaling in Mice.","authors":"Jiyeon Lee, Mi-Bo Kim, Changhee Kim, Chae Young Moon, Hyunju Kang, Jae-Kwan Hwang","doi":"10.4014/jmb.2604.04033","DOIUrl":"10.4014/jmb.2604.04033","url":null,"abstract":"Muscle atrophy involves the progressive loss of muscle mass and function, often linked to aging, disease, or inactivity. Although Leonurus japonicus and its active compound, leonurine, possess antioxidant and anti-inflammatory properties, their potential to improve muscle mass remains unknown. This study investigated the recovery-promoting effects and underlying mechanisms of a leonurine-standardized water extract of L. japonicus (LJW) and leonurine on muscle atrophy. We hypothesized that LJW and leonurine would promote recovery from muscle atrophy by activating the PI3K/Akt/mTOR pathway and suppressing FoxO3a-mediated proteolysis. Atrophy was induced in mice via 1-week hindlimb immobilization, followed by daily oral administration of LJW (150 or 300 mg/kg) or leonurine (30 mg/kg) for 1 week. LJW and leonurine not only improved exercise capacity and grip strength but also increased muscle volume, muscle mass, and cross-sectional area in the immobilized hindlimbs. At the molecular level, both treatments activated the mammalian target of rapamycin (mTOR) pathway for protein synthesis. They also increased forkhead box O3a (FoxO3a) phosphorylation and reduced FoxO3a-associated proteolytic markers, attenuating catabolic signaling by enhancing the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Furthermore, molecular docking analysis suggested that leonurine potentially interacts with the PI3K protein. Additionally, treatments reduced total nuclear factor kappa B (NF-κB) protein levels, which was accompanied by the downregulation of tumor necrosis factor α and interleukin 6. Taken together, LJW and leonurine enhanced recovery from immobilization-induced muscle atrophy, indicating their potential as novel therapeutic agents against muscle wasting.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604033"},"PeriodicalIF":3.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13246295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148157049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-Scale Microbiome Profiling of Brown Algae Identifies a Specific Vibrio aphrogenes Clade Strain That Promotes Gametophyte Settlement. 褐藻的大规模微生物组分析鉴定了一种促进配子体定居的特殊促性弧菌枝菌株。

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-01 DOI: 10.4014/jmb.2604.04061

Yurim Bae, Dong Jin Kim, Yerin Seo, Yeonjin Noh, Sujin Lee, Soon-Kyeong Kwon

{"title":"Large-Scale Microbiome Profiling of Brown Algae Identifies a Specific Vibrio aphrogenes Clade Strain That Promotes Gametophyte Settlement.","authors":"Yurim Bae, Dong Jin Kim, Yerin Seo, Yeonjin Noh, Sujin Lee, Soon-Kyeong Kwon","doi":"10.4014/jmb.2604.04061","DOIUrl":"10.4014/jmb.2604.04061","url":null,"abstract":"Marine macroalgae are foundation species of coastal ecosystems, maintaining close interactions with their microbiome for development and environmental adaptation. Although secure attachment to substrates is essential for both morphological development and survival, the potential causal link between microbial symbionts and this fundamental attachment process remains poorly understood. To address this gap, we integrated large-scale field sampling with functional validation to identify bacteria that influence the attachment of brown algae, specifically comparing attached and drifted individuals of Undaria pinnatifida and Ecklonia cava. Notably, Vibrio was consistently enriched in attached algae, whereas Marinomonas dominated drifted individuals. Translating these field observations into lab-scale validation, we isolated and evaluated their biofilm-formation capacity and impact on gametophyte settlement. A specific isolate belonging to the Vibrio aphrogenes clade exhibited superior biofilm formation and significantly enhanced algal abundance 2.57-fold in co-culture with U. pinnatifida gametophytes. These findings provide a scientific basis for developing bacterial inocula for seaweed seedling production and support broader macroalgae restoration strategies under changing environmental conditions.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604061"},"PeriodicalIF":3.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13265237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148226016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Photoaging and Anti-Melanogenic Effects of a Novel Lactiplantibacillus plantarum RKBP Isolated from Giant Centella asiatica. 巨积雪草植物乳杆菌RKBP的抗光老化和抗黑色素作用

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-01 DOI: 10.4014/jmb.2603.03011

Jiwon Seo, Eun Jin Sung, Do Yeong Son, Dae Sung Yoo, Dae Bang Seo, Myunghwa Kim, Chan Yoo

{"title":"Anti-Photoaging and Anti-Melanogenic Effects of a Novel Lactiplantibacillus plantarum RKBP Isolated from Giant Centella asiatica.","authors":"Jiwon Seo, Eun Jin Sung, Do Yeong Son, Dae Sung Yoo, Dae Bang Seo, Myunghwa Kim, Chan Yoo","doi":"10.4014/jmb.2603.03011","DOIUrl":"10.4014/jmb.2603.03011","url":null,"abstract":"Ultraviolet B (UVB) irradiation is a major contributor to photoaging and hyperpigmentation, leading to collagen damage and excessive melanin accumulation. This study investigated the anti-photoaging and anti-melanogenic effects of heat-killed L. plantarum RKBP isolated from Giant Centella asiatica. L. plantarum RKBP was prepared by thermal inactivation and evaluated for anti-photoaging effects using human dermal fibroblasts and three-dimensional (3D) human skin-equivalent tissues. The anti-melanogenic effects were assessed in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells, three-dimensional melanoma spheroids, and intrinsically pigmented 3D human skin-equivalent models. MAPK/AP-1 signaling, MMP-1 expression, collagen integrity, melanin content, and melanogenesis-related protein expression were analyzed. L. plantarum RKBP selectively suppressed UVB-induced ERK/JNK phosphorylation and AP-1-mediated MMP-1 expression, preserving collagen integrity in a 3D human skin-equivalent model. Treatment with L. plantarum RKBP reduced α-MSH-induced melanin production and decreased tyrosinase expression in B16F10 cells. L. plantarum RKBP also attenuated melanin accumulation in 3D melanoma spheroids and pigmented human skin-equivalent tissues. L. plantarum RKBP exerts anti-photoaging and anti-melanogenic effects across in vitro and 3D skin models. These findings support its potential as a postbiotic skin health ingredient.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2603011"},"PeriodicalIF":3.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13249599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon Dioxide Fixation Cascade for the Production of Single-Cell Protein by Clostridium ljungdahlii and Yarrowia lipolytica. 永格达氏梭菌和解脂耶氏菌产单细胞蛋白的二氧化碳固定级联。

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-01 DOI: 10.4014/jmb.2603.03009

Gengjie Zhang, Mingchi Lai, Xuanyue Lu, Chuanzhao Wang, Fuli Li, Wenzhu Tang, Ziyong Liu

{"title":"Carbon Dioxide Fixation Cascade for the Production of Single-Cell Protein by Clostridium ljungdahlii and Yarrowia lipolytica.","authors":"Gengjie Zhang, Mingchi Lai, Xuanyue Lu, Chuanzhao Wang, Fuli Li, Wenzhu Tang, Ziyong Liu","doi":"10.4014/jmb.2603.03009","DOIUrl":"10.4014/jmb.2603.03009","url":null,"abstract":"Clostridium species and yeast single-cell protein (SCP), produced via industrial fermentation, offer a sustainable, high-protein alternative to conventional sources while advancing circular economy goals through CO2 valorization. Here, we developed an integrated two-stage bioprocess for SCP production from CO2. First, Clostridium ljungdahlii converted CO2 and H2 into SCP and acetate via gas fermentation. Optimizing nitrogen and sulfur feeding based on acetate synthesis rate increased acetate titer from 6 to 53.2 g/L and biomass from 1.1 to 2.8 (OD600). The fermentation broth was then directly used by Yarrowia lipolytica. Without pH control, dry cell weight (DCW) reached 4.7 g/L (67.0% protein, 6.5% lipid). With H2SO4-maintained pH 7, DCW reached 17.6 g/L (75.0% protein, 11.1% lipid). Notably, pH control using acetate yielded the highest DCW of 74.6 g/L (57.0% protein, 10.5% lipid). It should be noted that the highest biomass titer was achieved only when 130 g/L external acetate was added with pH control. This work demonstrates an efficient strategy for converting CO2 into valuable microbial protein, advancing scalable SCP production.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2603009"},"PeriodicalIF":3.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13259618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148226035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Molecular Repertoire of Akkermansia muciniphila: From Mechanistic Insights to Precision Biotherapeutics. 揭示嗜黏液阿克曼氏菌的分子库：从机制的见解到精确的生物治疗。

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-06-01 DOI: 10.4014/jmb.2604.04017

Tae-Hwan Kim, Su-Man Kim, Myung Hee Kim

{"title":"Unveiling the Molecular Repertoire of Akkermansia muciniphila: From Mechanistic Insights to Precision Biotherapeutics.","authors":"Tae-Hwan Kim, Su-Man Kim, Myung Hee Kim","doi":"10.4014/jmb.2604.04017","DOIUrl":"10.4014/jmb.2604.04017","url":null,"abstract":"The gut microbiota has been established as a cornerstone of host physiological homeostasis. Among its diverse members, Akkermansia muciniphila has gained significant prominence as a premier next-generation probiotic candidate, demonstrating broad efficacy in alleviating metabolic disorders, inflammatory bowel disease, and neurodegenerative conditions. While early research primarily focused on clinical correlations and population abundance, recent scientific paradigms have shifted toward elucidating the underlying molecular mechanisms and specific host-microbe interactions. This review provides a comprehensive synthesis of recent breakthroughs in identifying A. muciniphila-derived bioactive effectors, including structural components, secreted enzymes, and signaling peptides. We examine how these molecular postbiotics orchestrate host health by reinforcing intestinal barrier integrity, modulating systemic immune responses, and reprogramming the tumor microenvironment. By integrating these multifaceted modes of action into a unified framework, we evaluate the therapeutic potential of both live bacteria and cell-free derivatives. Furthermore, we address the critical challenges regarding strain-specific efficacy and ecological impacts, providing a strategic roadmap for the clinical translation of A. muciniphila into the next frontier of precision microbiome medicine.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604017"},"PeriodicalIF":3.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13246296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148157010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATPIF1 Deficiency Significantly Alleviates Citrobacter rodentium-Induced Colitis in Mice. ATPIF1缺乏可显著减轻鼠柠檬酸杆菌诱导的小鼠结肠炎

IF 3.4 4区生物学

Journal of microbiology and biotechnology Pub Date : 2026-05-29 DOI: 10.4014/jmb.2604.04015

Haoyu Yang, Ziqi Li, Dong Yan, Min Li, Lingyun Xu, Yuxin Wang, Genshen Zhong, Minna Wu

{"title":"ATPIF1 Deficiency Significantly Alleviates Citrobacter rodentium-Induced Colitis in Mice.","authors":"Haoyu Yang, Ziqi Li, Dong Yan, Min Li, Lingyun Xu, Yuxin Wang, Genshen Zhong, Minna Wu","doi":"10.4014/jmb.2604.04015","DOIUrl":"10.4014/jmb.2604.04015","url":null,"abstract":"Mitochondrial ATP synthase inhibitory factor 1 (ATPIF1) regulates cellular energy metabolism and has been implicated in inflammatory disorders. However, its role in infection-associated colitis remains unclear. This study aimed to investigate the effects of ATPIF1 on host susceptibility and inflammation in a Citrobacter rodentium-induced infectious colitis model. ATPIF1 knock out (KO) and wild type (WT) mice were orally gavaged with C. rodentium to induce infectious colitis. Body weight, disease activity index (DAI), and colon length, histopathology, barrier function, inflammatory markers, and gut microbiota were assessed using standard approaches, including immunohistochemistry, RT-qPCR, Western blotting, and 16S rRNA sequencing. ATPIF1 deficiency alleviated C. rodentium-induced colitis, as evidenced by reduced weight loss, lower DAI scores, and attenuated colon shortening. KO mice preserved epithelial architecture, exhibited increased numbers of goblet cells and ZO-1 mRNA expression, indicating an intact mucosal barrier. Furthermore, KO mice showed reduced infiltration of inflammatory cells, decreased expression of IL-1β and TNF-α, and reduced activation of the NLRP3 inflammasome pathway, although whether this is a direct effect remains unclear. Microbiota analysis also revealed that ATPIF1 deficiency stabilized microbiota composition and reduced pathogenic expansion. ATPIF1 deficiency exhibited a protective phenotype in C. rodentium-induced colitis, accompanied by reduced inflammation, preserved barrier function, enhanced pathogen clearance, and stabilized microbiota composition. ATPIF1 may represent a potential therapeutic target in infection-associated colitis.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604015"},"PeriodicalIF":3.4,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13249600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0