Journal of microbiology and biotechnology最新文献_第10页

Design and Synthesis of Hydroxamate-Based Matrix Metalloproteinase-2 Inhibitors for Anti-Photoaging. 羟基酸盐基基质金属蛋白酶-2抗光老化抑制剂的设计与合成。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-02-10 DOI: 10.4014/jmb.2412.12027

Jin Young Lee, Geunhyuk Jang, Yung Hyup Joo, Joonho Choi, Dong-Woo Lee, Jae Won Yoo

引用次数: 0

Fermented Protaetia brevitarsis Larvae Alleviates High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in C57BL/6 Mice via Regulation of Lipid Accumulation and Inflammation. 发酵短弧菌幼虫通过调节脂质积累和炎症减轻高脂饮食诱导的C57BL/6小鼠非酒精性脂肪肝

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-02-10 DOI: 10.4014/jmb.2409.09025

Hyo Lim Lee, Jong Min Kim, Min Ji Go, Han Su Lee, Ju Hui Kim, In Young Kim, Geum-Su Seong, Ho Jin Heo

{"title":"Fermented Protaetia brevitarsis Larvae Alleviates High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in C57BL/6 Mice via Regulation of Lipid Accumulation and Inflammation.","authors":"Hyo Lim Lee, Jong Min Kim, Min Ji Go, Han Su Lee, Ju Hui Kim, In Young Kim, Geum-Su Seong, Ho Jin Heo","doi":"10.4014/jmb.2409.09025","DOIUrl":"10.4014/jmb.2409.09025","url":null,"abstract":"Non-alcoholic fatty liver disease (NAFLD), characterized by hepatic steatosis and hepatitis, is the most frequently encountered complication of type 2 diabetes mellitus (T2DM). Due to its hepatoprotective, anti-obesity, antioxidant, and anti-inflammatory effects, Protaetia brevitarsis (P. brevitarsis) larvae have been used as traditional medicine to treat liver diseases since ancient times. Therefore, this study was conducted to confirm the positive effect of fermented P. brevitarsis larvae (FPB) on NAFLD. The results showed that high-fat diet (HFD)-induced dysglycemia was improved by treatment with FPB as determined by testing for fasting blood glucose and oral glucose tolerance. The weight of liver and white adipose tissue and the levels of serum lipid, hepatotoxicity, and nephrotoxicity indicators were reduced by FPB. In addition, oxidative stress and mitochondrial dysfunction caused by HFD were improved by FPB. In a similar manner, HFD-induced hepatic steatosis was prevented by FPB through regulation of the AMP-activated protein kinase pathway and serum lipid profile. HFD-induced hepatitis and apoptosis were ameliorated by FPB via the nuclear factor-kappa B pathway and the B-cell lymphoma 2 protein family. In conclusion, this study suggests the potential for application of FPB as a prophylactic agent for treatment of NAFLD through suppression of lipid accumulation and inflammation in the liver.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2409025"},"PeriodicalIF":2.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deoxybouvardin Glucoside Induces Apoptosis in Oxaliplatin-Sensitive and -Resistant Colorectal Cancer Cells via Reactive Oxygen Species-Mediated Activation of JNK and p38 MAPK. 脱氧布瓦丁苷通过活性氧介导的JNK和p38 MAPK激活诱导奥沙利铂敏感和耐药结直肠癌细胞凋亡

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-01-30 DOI: 10.4014/jmb.2410.10008

Seung-On Lee, Sang Hoon Joo, Jisu Park, Quan T Khong, Si Yeong Seo, Goo Yoon, Jin Woo Park, MinKyun Na, Jung-Hyun Shim

{"title":"Deoxybouvardin Glucoside Induces Apoptosis in Oxaliplatin-Sensitive and -Resistant Colorectal Cancer Cells via Reactive Oxygen Species-Mediated Activation of JNK and p38 MAPK.","authors":"Seung-On Lee, Sang Hoon Joo, Jisu Park, Quan T Khong, Si Yeong Seo, Goo Yoon, Jin Woo Park, MinKyun Na, Jung-Hyun Shim","doi":"10.4014/jmb.2410.10008","DOIUrl":"10.4014/jmb.2410.10008","url":null,"abstract":"The roots of Rubia spp. (Rubiaceae) have been employed to treat hematemesis, inflammatory disease, and tumor. Cyclohexapeptides derived from Rubia spp. have been reported to have antitumor potential; however, the mechanism of action for their antitumor activity remains unclear. We aimed to examine the antitumor effect of deoxybouvardin glucoside (DBG), a cyclohexapeptide from Rubia spp. on oxaliplatin (Ox)-resistant human HCT116 colorectal cancer (CRC) cells. Cell viability in the presence of DBG was monitored using an MTT viability assay, and flow cytometry was used to analyze changes in apoptosis, cell cycle, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) activity. The antiproliferative activity involved apoptosis and phosphorylation of JNK and p38 MAPK. Inhibition of JNK and p38 MAPK by specific inhibitors prevented DBG-induced apoptosis, underscoring the close involvement of these kinases. Further, DBG induced cell cycle arrest in CRC cells at the G2/M phase by regulating the p21, p27, cyclin B1, and cdc2 proteins. DBG-induced apoptosis was accompanied mitochondrial membrane depolarization, resulting in cytochrome c release into the cytoplasm and caspase activation. Remarkably, DBG induced apoptosis by generating high ROS levels. The mediation of apoptosis by increased ROS generation was confirmed by pretreatment with the ROS scavenger N-acetyl cysteine (NAC). Collectively, DBG exhibited anticancer activity against both Ox-sensitive and Ox-resistant CRC cells by targeting JNK and p38 MAPK, inducing cell cycle arrest, elevating cellular ROS levels, and disrupting MMP. This study suggests that DBG has the potential to be utilized as a therapeutic agent for treating Ox-resistant CRC.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2410008"},"PeriodicalIF":2.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exo- and Endo-1,5-α-L-Arabinanases and Prebiotic Arabino-Oligosaccharides Production. 外显和内显1,5-α- l -阿拉伯糖酶和益生元阿拉伯寡糖的生产。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-01-13 DOI: 10.4014/jmb.2412.12052

Ye-Rin Ju, Su Been Im, Da Eun Jung, Min Jeong Son, Chan-Young Park, Min Ho Jeon, Ju Hee Hwang, Soo Jung Lee, Tae-Jip Kim

{"title":"Exo- and Endo-1,5-α-L-Arabinanases and Prebiotic Arabino-Oligosaccharides Production.","authors":"Ye-Rin Ju, Su Been Im, Da Eun Jung, Min Jeong Son, Chan-Young Park, Min Ho Jeon, Ju Hee Hwang, Soo Jung Lee, Tae-Jip Kim","doi":"10.4014/jmb.2412.12052","DOIUrl":"10.4014/jmb.2412.12052","url":null,"abstract":"There is growing interest in pentose-based prebiotic oligosaccharides as alternatives to traditional hexose-based prebiotics. Among these, arabino-oligosaccharides (AOS), derived from the enzymatic hydrolysis of arabinan polymers, have gained significant attention. AOS can selectively stimulate the growth of beneficial gut bacteria, including Bifidobacterium and Bacteroides species, and contribute to health-benefit functions such as blood sugar control, positioning AOS as a promising synbiotic candidate. For the industrial production of AOS, the development of efficient enzymatic processes is essential, with exo- and endo-1,5-α-L-arabinanases (exo- and endo-ABNs) playing a crucial catalytic role. Most ABNs belong to the glycoside hydrolase (GH) family 43, characterized by a five-bladed β-propeller fold structure. These enzymes hydrolyze internal α-1,5-L-arabinofuranosidic linkages, producing AOS with varying degrees of polymerization. Some ABNs GH43 were known to exhibit exo-type hydrolytic modes of action, producing specific AOS products such as arabinotriose. Additionally, exo-ABNs from GH93, which feature a six-bladed β-propeller fold, exclusively release arabinobiose through their exo-type catalytic mechanism. This review represents the first comprehensive analysis of exo- and endo-ABNs, offering scientific insights into their biotechnological potential for AOS production. It systematically compares enzyme classification, structural differences, catalytic mechanisms, paving the way for innovative applications in health, food, and pharmaceutical industries.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412052"},"PeriodicalIF":2.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of Melanin Synthesis and Inflammation by Exosomes Derived from Leuconostoc mesenteroides DB-14 Isolated from Camellia japonica Flower. 山茶花肠系膜leconostoc DB-14外泌体对黑色素合成和炎症的抑制作用

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-01-06 DOI: 10.4014/jmb.2411.11080

Byeong Min Choi, Tae-Jin Park, HuSang Harry Lee, Hyehyun Hong, Won-Jae Chi, Seung-Young Kim

{"title":"Inhibition of Melanin Synthesis and Inflammation by Exosomes Derived from Leuconostoc mesenteroides DB-14 Isolated from Camellia japonica Flower.","authors":"Byeong Min Choi, Tae-Jin Park, HuSang Harry Lee, Hyehyun Hong, Won-Jae Chi, Seung-Young Kim","doi":"10.4014/jmb.2411.11080","DOIUrl":"10.4014/jmb.2411.11080","url":null,"abstract":"Leuconostoc mesenteroides is a lactic acid bacteria found in fermented products. In our previous study, L. mesenteroides was isolated from Camellia japonica flowers, and its acid tolerance and antibacterial properties were thoroughly investigated. This study focuses on the inhibition of melanin synthesis and inflammation of exosomes derived from L. mesenteroides. Moreover, L. mesenteroides exosomes (DB-14 exosome) exhibited significant inhibitory effects on inflammation and melanogenesis. At concentrations of 4.44 × 108, 8.88 × 108, and 1.78 × 109 particles/ml, the exosomes reduced nitric oxide and prostaglandin E2 activity while maintaining the growth of RAW 264.7 macrophages. In addition, proinflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha, were rarely expressed, and western blot revealed that L. mesenteroides DB-14 derived exosomes inhibited inducible nitric oxide synthase and cyclooxygenase-2 expression. Moreover, the exosomes had no toxic effects on B16F10 melanoma cells at concentrations of 1.78 × 109, 3.55 × 109, and 7.10 × 109 particles/ml, and they suppressed melanogenesis by reducing tyrosinase activity. Furthermore, western blot analysis demonstrated that microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase related protein (TRP)-1, and TRP-2 were evidently reduced, ultimately repressing melanin production. Moreover, MITF expression was inhibited by reduced mitogen-activated protein kinase and protein kinase B phosphorylation levels. Overall, this study proves the efficacy of the novel DB-14 exosome as a strong lightening and anti-inflammatory agent.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411080"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss in Pluripotency Markers in Mesenchymal Stem Cells upon Infection with Chlamydia trachomatis. 感染沙眼衣原体后间质干细胞多能性标志物的丧失

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-12-07 DOI: 10.4014/jmb.2406.06023

Munir A Al-Zeer, Mohammad Abu Lubad

{"title":"Loss in Pluripotency Markers in Mesenchymal Stem Cells upon Infection with Chlamydia trachomatis.","authors":"Munir A Al-Zeer, Mohammad Abu Lubad","doi":"10.4014/jmb.2406.06023","DOIUrl":"10.4014/jmb.2406.06023","url":null,"abstract":"The intracellular pathogen Chlamydia trachomatis can inflict substantial damage on the host. Notably, Chlamydia infection is acknowledged for its precise modulation of diverse host signaling pathways to ensure cell survival, a phenomenon intricately connected to genetic regulatory changes in host cells. To monitor shifts in gene regulation within Chlamydia-infected cells, we employed mesenchymal stem cells (MSCs) as a naïve, primary cell model. Utilizing biochemical methods and imaging, our study discloses that acute Chlamydia infection in human MSCs leads to the downregulation of transcription factors Oct4, Sox2, and Nanog, suggesting a loss of pluripotency markers. Conversely, pluripotency markers in MSCs were sustained through treatment with conditioned medium from infected MSCs. Additionally, there is an augmentation in alkaline phosphatase activity, along with elevated Sox9 and CD44 mRNA expression levels observed during acute infection. A comprehensive screening for specific cell markers using touchdown PCR indicates an upregulation of mRNA for the early chondrogenesis gene Sox9 and a decrease in mRNA for the MSC marker vimentin. Real-time PCR quantification further corroborates alterations in gene expression, encompassing increased Sox9 and CD44 mRNA levels, alongside heightened alkaline phosphatase activity. In summary, the infection of MSCs with C. trachomatis induces numerous genetic deregulations, implying a potential trend towards differentiation into chondrocytes. These findings collectively underscore a targeted impact of Chlamydia on the gene regulations of host cells, carrying significant implications for the final fate and differentiation of these cells.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2465-2473"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triple-Branch Catalytic Assembly DNAzyme Motivated DNA Tweezer for Sensitive and Reliable mecA Gene Detection in Staphylococcus aureus. 用于灵敏可靠地检测金黄色葡萄球菌中 mecA 基因的三分支催化组装 DNA 酶动机 DNA 镊子

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-01 DOI: 10.4014/jmb.2409.09008

Xiaoyang Li, Meiyan Xu, Fangmin Gan, Hui Zhao

{"title":"Triple-Branch Catalytic Assembly DNAzyme Motivated DNA Tweezer for Sensitive and Reliable mecA Gene Detection in Staphylococcus aureus.","authors":"Xiaoyang Li, Meiyan Xu, Fangmin Gan, Hui Zhao","doi":"10.4014/jmb.2409.09008","DOIUrl":"10.4014/jmb.2409.09008","url":null,"abstract":"Staphylococcus aureus (S. aureus, SA) is one of the most common bacteria in nosocomial infections. Sensitive and efficient analysis of methicillin-resistance of SA is crucial for improving the nursing performance of pneumonia. However, methicillin-resistance analysis with favorable sensitivity and specificity in an enzyme-free manner remains a huge challenge. This paper presents the development of a new fluorescent biosensor for detecting mecA gene using a triple-branch catalytic hairpin assembly (CHA) triggered DNAzyme switch-based DNA tweezer. The SA from the samples are immobilized on the plate's surface using the protein A antibody. The biosensor possesses several key features. Firstly, it utilizes dual signal amplification processes, specifically the triple-branch CHA and DNAzyme controlled DNA tweezer-based signal recycling, to enable mecA detection on the plate. This design enhances the method's sensitivity, resulting in a low limit of detection of 1.5 fM. Secondly, the biosensor does not rely on enzymes for mecA analysis, ensuring a high level of stability during target analysis. Lastly, the method demonstrates a remarkable selectivity by accurately distinguishing target sequences from non-target sequences. The proposed biosensor, which does not require enzymes and has a high level of sensitivity, offers a viable platform for the rapid and simple quantification of mecA in SA.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2450-2456"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Icariside II Preparation from Icariin Separated from Epimedium Herbal Extract Powder by a Special Icariin Glycosidase. 用一种特殊的淫羊藿苷糖苷酶从淫羊藿草药提取物粉末中分离出淫羊藿苷制备淫羊藿苷 II。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-24 DOI: 10.4014/jmb.2408.08046

Xinyu Liu, Siyu Xu, Chunying Liu, Zhenghao Wang, Bo Wu, Meijuan Guo, Changkai Sun, Hongshan Yu

{"title":"Icariside II Preparation from Icariin Separated from Epimedium Herbal Extract Powder by a Special Icariin Glycosidase.","authors":"Xinyu Liu, Siyu Xu, Chunying Liu, Zhenghao Wang, Bo Wu, Meijuan Guo, Changkai Sun, Hongshan Yu","doi":"10.4014/jmb.2408.08046","DOIUrl":"10.4014/jmb.2408.08046","url":null,"abstract":"In this study, icariside II was prepared from icariin by a special enzyme. The yield of the substrate icariin from a powdered extract of the popular herb Epimedium was 16.9%. The enzyme, which was produced from Aspergillus sp.y48 fermentation, hydrolyzes icariin to icariside II and was characterized. The molecular weight was 75 kDa, while the optimum temperature and pH were 45°C and 5.0. The purified enzyme hydrolyzed the 7-O-glucoside of icariin or epimedin A, B, and C to icariside II, or sagittatoside A, B, and C, respectively, and further hydrolyzed the terminal 3-O-xyloside of sagittatoside B to icariside II. The enzyme is a special icariin glycosidase that hydrolyzed icariin to icariside II at low cost. Based on the crude enzyme's reaction dynamics, the optimal conditions for icariside II preparation showed that 2% icariin reacted at 45°C for 6 to 9 h. Here, we obtained 13.3 g icariside II and 0.45 g of the by-product icaritin from 20 g icariin. The icariside II molar yield was 87.4%, the by-product icaritin yield was 4.1%, and the total molar yield was 91.5%. Therefore, icariside II was resoundingly prepared from an icariin glycosidase of an Epimedium extract using a non-GMO, crude enzyme from Aspergillus sp.y48. The obtained icariside II and the by-product icaritin can be directly applied in the production of cosmetics and pharmaceuticals.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2683-2692"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory Effects of Cryptotanshinone and Dihydrotanshinone I on Intracellular Trafficking of Viral Glycoproteins. 隐丹参酮和二氢丹参酮I对病毒糖蛋白细胞内转运的抑制作用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-25 DOI: 10.4014/jmb.2409.09050

Makoto Muroi, Dong-Sun Lee

{"title":"Inhibitory Effects of Cryptotanshinone and Dihydrotanshinone I on Intracellular Trafficking of Viral Glycoproteins.","authors":"Makoto Muroi, Dong-Sun Lee","doi":"10.4014/jmb.2409.09050","DOIUrl":"10.4014/jmb.2409.09050","url":null,"abstract":"Antiviral agents that target the viral envelope surface glycoproteins can disrupt the interactions between the viral glycoproteins and host cell receptors, thereby preventing viral entry into host cells. However, the mechanisms underlying glycoprotein processing and cellular trafficking have not been fully elucidated. In this study, we aimed to investigate the mechanism of action of cryptotanshinone (CTN) and dihydrotanshinone I (DTN) as inhibitors of viral glycoprotein trafficking, by assessing their inhibitory action on syncytium formation and cytopathic effects. CTN and DTN were isolated and characterized from Salvia miltiorrhiza; they effectively inhibited syncytium formation in Newcastle disease virus-infected baby hamster kidney cells. Both compounds inhibited the transport of viral G-proteins to the cell surface, resulting in intracellular accumulation. These results suggest that CTN and DTN are potential glycoprotein trafficking inhibitors that function at the Golgi apparatus. Overall, our results indicate that CTN and DTN suppress intracellular glycosylation by competing as inhibitors of glycosylation trafficking.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2457-2464"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of Codium fragile Extract against Acetaminophen-Induced Liver Injury. 脆弱钠提取物对对乙酰氨基酚引起的肝损伤的保护作用

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-25 DOI: 10.4014/jmb.2409.09061

Yea-Lim Lee, Ji-Yun Lee, Joo-Woong Park, Jin Lee, Hyun-Hoo Lee, Dae-Hee Lee

{"title":"Protective Effects of Codium fragile Extract against Acetaminophen-Induced Liver Injury.","authors":"Yea-Lim Lee, Ji-Yun Lee, Joo-Woong Park, Jin Lee, Hyun-Hoo Lee, Dae-Hee Lee","doi":"10.4014/jmb.2409.09061","DOIUrl":"10.4014/jmb.2409.09061","url":null,"abstract":"Acetaminophen (APAP) is a well-known analgesic used globally. Generally, APAP has been proven to be safe and effective at therapeutic doses; however, it can cause serious liver damage when administered at high levels. We prepared Codium fragile extract (CFE) using the seaweed C. fragile and confirmed that the CFE contains a substance called Loliolide with antioxidant activity. We performed the present study to determine whether CFE protects HEPG2 cells and BALB/c mice from oxidative stress-induced liver damage. We confirmed that CFE and Loliolide were non-cytotoxic and protected against liver damage by reducing the activities of ALT and AST, which were increased by APAP treatment, and that CFE reduced the mRNA expression of inflammatory cytokines TNF-α and IL-6 and inhibited the phosphorylation of ERK and p38 in HEPG2 cells as determined by RT-PCR and Western blot analyses. Furthermore, the TNF-α and IL-6 levels, which were increased after APAP treatment in BALB/c mice, decreased after CFE treatment. Therefore, we demonstrated that CFE exerts a protective effect against APAP-induced liver injury by suppressing the inflammatory response through anti-inflammatory activity. Our findings provide new perspectives for developing functional foods that utilize seaweeds to improve liver function.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2675-2862"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0