Journal of microbiology and biotechnology最新文献

{"title":"Schisandrin C Improves Chronic Stress-Induced Dyslipidemia in Mice by Regulating Pyroptosis and Autophagy Levels.","authors":"Yuling Liu, Wenjun Lu, Yue Huang, Chaozhi Xu, Wanhua Wu, Zongyi Zhang, Yulin Zhang, Shanqian Li, Guixian Wu, Doudou Wang, Yao Xu, Meimei Zheng, Hongxian Wu, Lina Hu","doi":"10.4014/jmb.2501.01041","DOIUrl":"10.4014/jmb.2501.01041","url":null,"abstract":"<p><p>Chronic stress emerges as a significant risk factor for metabolic disorders, including hyperlipidemia and fatty liver disease. Schisandrin C (SchC), an essential component of Schisandra chinensis lignans, is known to possess lipid-lowering and liver-protective properties. However, the precise mechanisms underlying its action remain incompletely understood. We hypothesized that SchC exerts a protective effect on chronic stress-induced dyslipidemia in mice by modulating hepatic autophagy and pyroptosis. In this study, a dual model was established for 12 weeks, combining chronic stress with a high-fat diet (HFD). SchC or simvastatin were given respectively starting at week 10. Blood, epididymal white adipose tissue and liver were collected for further analysis. Behavioral tests and lipid levels showed that the dual model construction of HFD and chronic stress used in this study was successful in placing mice in a stressful environment and triggering dyslipidemia. SchC intervention effectively impeded the accumulation of eWAT and reduced the increased lipid levels in mice induced by chronic stress. The expression levels of the pyroptosis marker Caspase-1 and other inflammatory factors NLRP3, IL-1β, and IL-18 were significantly reduced in the liver tissues of chronically stressed mice under SchC treatment. Additionally, SchC significantly reduced expression levels of autophagy-related factors such as Beclin-1, the LC3-B/A ratio, P62, and markers related to the autophagy pathway (PI3K/AKT/mTOR). SchC effectively improves dyslipidemia through various pathways including inhibiting the PI3K/AKT/mTOR pathway, promoting autophagy, reducing pyroptosis. This provides a solid theoretical foundation for the clinical application of SchC in the treatment of dyslipidemia and related diseases.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501041"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic Plantaricins Derived from <i>Lactiplantibacillus plantarum</i> KM2 Induce Cell Lysis of <i>Listeria monocytogenes</i>.","authors":"Seung-Eun Oh, Sojeong Heo, Minkyeong Kim, Yura Moon, Sumin Lee, Chaerin Park, Huieun Sung, Gawon Lee, Jina Kim, Moon-Hee Sung, Do-Won Jeong","doi":"10.4014/jmb.2504.04006","DOIUrl":"10.4014/jmb.2504.04006","url":null,"abstract":"<p><p><i>Listeria monocytogenes</i> is a deadly foodborne pathogen that presents significant challenges in food safety management due to its high resilience in various environments. This study evaluated antibacterial activities of synthetic plantaricins derived from <i>Lactiplantibacillus plantarum</i> KM2 against <i>L. monocytogenes</i>. The minimum inhibitory concentrations of the five synthetic plantaricin combinations-spPlnA, spPlnJ, spPlnE&F, spPlnE&J, and spPlnJ&K-were 1.4, 1.5, 1.8, 1.6, and 1.6 μg/ml, respectively, Raman spectroscopy and transmission electron microscopy demonstrated that the synthetic plantaricins induced morphological alterations, including cell wall damage and cell lysis. Notably, spPlnE&F and spPlnE&J were shown to effectively disrupt the bacterial cell wall. Furthermore, assessments of antibacterial stability under varying temperatures and pH conditions showed that plantaricin combinations maintained their efficacy at pH levels ranging from 4 to 7 and temperatures below 40°C. These findings suggest that synthetic plantaricins have strong potential as natural preservatives in food applications, offering an effective approach to targeting specific pathogens and enhancing food safety.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504006"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Serum Uric Acid Levels and Salivary Microbiota in Patients with Obstructive Sleep Apnea.","authors":"Yujia Lu, Wanxin Zhang, Min Yu, Xuehui Chen, Chunyan Liu, Xuemei Gao","doi":"10.4014/jmb.2503.03042","DOIUrl":"10.4014/jmb.2503.03042","url":null,"abstract":"<p><p>The microbiota is associated with obstructive sleep apnea (OSA) and hyperuricemia (HUA), but the relationship between oral microbiota and OSA-related HUA remains unclear. Our study investigated salivary microbiota differences between individuals with OSA and those with both OSA and HUA, and explored the link between oral microbiome alterations and uric acid fluctuations in OSA patients. Seventy-two adults were divided into four groups: controls (n = 20, 33.75 ± 9.46 years), OSA (n = 23, 44.08 ± 13.70 years), OSA with comorbid HUA (OSA+HUA, n = 22, 40.18 ± 9.58 years), and OSA with medication-controlled HUA (n = 7, 44.56 ± 15.14 years). Salivary microbiota and proteomic profiles were analyzed using 16S rRNA sequencing and Astral DIA. OSA and OSA+HUA showed reduced alpha-diversity compared to controls. The OSA+HUA group had increased <i>Oribacterium</i> abundance relative to the OSA group, which decreased after uric acid treatment, whereas <i>Rothia</i>, <i>Capnocytophaga</i>, and <i>Aggregatibacter</i> showed the opposite trend. 104 differentiated proteins were identified between the OSA and OSA+HUA groups. <i>Oribacterium</i> was positively correlated with several antioxidant proteins, while the other three genera were negatively correlated. This study identifies non-invasive biomarkers in the OSA+HUA group, as the first of its kind, highlighting the role of oral microbiota in future research and therapies.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503042"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Makgeolli Lees as a Novel Prebiotic Candidate: Effects on Human Gut Microbiota and Metabolites.","authors":"Jun Hoi Kim, Hwa Rin Kim, Hyunbin Seong, Seung Hee Han, Geonhee Kim, Yuri Choi, Nam Soo Han","doi":"10.4014/jmb.2504.04020","DOIUrl":"10.4014/jmb.2504.04020","url":null,"abstract":"<p><p>Makgeolli lees (ML), an underutilized byproduct of traditional Korean rice wine production, contains abundant indigestible carbohydrates and microbial residues. This study evaluates the prebiotic potential of ML using a simulated human digestion and <i>in vitro</i> fecal fermentation model. <i>In vitro</i> digestion showed that 61.30% of total carbohydrates and 89.92% of crude protein remained undigested. During <i>in vitro</i> fecal fermentation, treatment with ML induced compositional changes in the gut microbiota, including increased abundances of <i>Bifidobacterium bifidum</i> (Δ 0.37 ± 0.09%), <i>Prevotella copri</i> (Δ 0.98 ± 0.24%), and <i>Bacteroides uniformis</i> (Δ 0.46 ± 0.06%), along with a decrease in <i>Bacteroides ovatus</i> (Δ -2.14 ± 1.51%). In addition, SCFA concentrations were elevated after 12 h of fermentation, with acetate at 84.80 ± 4.88 mM, propionate at 43.75 ± 2.74 mM, and butyrate at 20.22 ± 0.71mM. The alterations in microbial composition and associated metabolite profiles were comparable to those observed following fructooligosaccharides (FOS) supplementation. These findings indicate that ML function as a fermentable substrate that selectively enriches health-associated gut microbiota and enhances the production of beneficial microbial metabolites, thereby supporting their potential application as a sustainable and cost-effective prebiotic candidate in dietary applications.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504020"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modified Renshen Wumei Decoction Improves Qi-Yin Deficiency Diarrhea by Regulating the Gut Microbiotas and Metabolites in Rats.","authors":"Junqi Zhao, Zhiwei Guan, Shuhua Fan, Jianli Qiu, Yan Xu, Qiong Zhao","doi":"10.4014/jmb.2412.12037","DOIUrl":"10.4014/jmb.2412.12037","url":null,"abstract":"<p><p>The hub metabolites and hub microorganisms that play an important role in the intervention of Qi-yin deficiency syndrome with diarrhea by Modified Renshen Wumei Decoction (MRWD) are still unclear. Therefore, we explored it based on multi-omics analysis. A total of 32 Sprague-Dawley rats were collected and randomly allocated into different groups. Subsequently, blood samples and fecal samples were collected from all 32 rats for non-targeted metabolome sequencing and 16S rRNA gene sequencing respectively. Among them, fecal samples of 24 rats were also used for targeted metabolomics sequencing (6 rats in each group). Pathological findings, and D-lactate, diamine oxidase (DAO), aquaporin 3 (AQP3), and aquaporin 8 (AQP8) levels in serum and colon samples were evaluated after the 21-day trial, and the results showed that diarrhea caused intestinal injury, which was ameliorated by infusion of MRWD. Based on multi-omics sequencing analysis, five microorganisms were superior to the positive control in MRWD intervention. Moreover, Eisenbergiella, Corynebacterium, and unidentified Oscillospiraceae exhibited significant discriminatory capabilities between groups C and D, groups B and C, as well as groups A and C; thus they were identified as hub microorganisms. On the other hand, significant differences in metabolites were observed among different groups with respect to the metabolome. These metabolites exhibited significant enrichment in metabolic pathways such as Butanoate metabolism, Propanoate metabolism, and Pyruvate metabolism. Further correlation analysis revealed that 2-Methylbutyrate was identified as a hub metabolites associated with blood and fecal microorganisms. Moreover, there were complex regulatory relationships between these hub microbes and hub metabolites.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412037"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EIF4A3-Induced Circ_0092278 Enhances Papillary Thyroid Cancer Cell Malignancy by the PI3K/Akt/mTOR Signaling Pathway.","authors":"Mengjiang Liu, Linghui Zhang, Zhaodan Yan","doi":"10.4014/jmb.2501.01010","DOIUrl":"10.4014/jmb.2501.01010","url":null,"abstract":"<p><p>Circular RNA (circRNA) plays a regulatory role in cancer progression, but the role of circ_0092278 in papillary thyroid cancer (PTC) is unclear. In this research, we aimed to reveal the effect of circ_0092278 on PTC progression as well as its interaction with the eukaryotic translation initiation factor 4A3 (EIF4A3). The expression of circ_0092278 in PTC samples was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). Cell experiments were conducted to assess the effects of circ_0092278 on PTC cell viability, migration, and invasion. Furthermore, key proteins involved in the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway were examined using western blotting. Additionally, a xenograft model in nude mice was used to investigate the <i>in vivo</i> role of circ_ 0092278. The interactions between EIF4A3 and circ_0092278 were analyzed through RNA-binding protein immunoprecipitation and qRT-PCR assays. Circ_0092278, located in the cell cytoplasm, was significantly upregulated in PTC. Knockdown of circ_0092278 reduced PTC cell proliferation, migration, invasion, and tumor growth by inhibiting the PI3K/Akt/mTOR pathway. Conversely, overexpression of circ_0092278 prompted PTC cell malignancy by activating the PI3K/Akt/mTOR pathway. Additionally, EIF4A3 was found to bind to MINK1 (circ_0092278 linear gene), thereby enhancing the expression of circ_0092278. Circ_0092278, controlled by EIF4A3, promotes PTC progression by activating the PI3K/Akt/mTOR pathway. Our findings indicate that targeting the EIF4A3-circ_0092278 axis may provide a novel approach for treating PTC.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501010"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digestibility and Quality Characteristics of <i>Sulgidduk</i> (a Traditional Korean Rice Cake) Prepared with Malic Acid-Treated Wheat Starch.","authors":"Gyeong A Jeong, Inae Lee, Chang Joo Lee","doi":"10.4014/jmb.2505.03037","DOIUrl":"10.4014/jmb.2505.03037","url":null,"abstract":"<p><p>With rising obesity rates worldwide and growing health awareness, the demand for low-calorie, blood sugar-controlling products is heightening. Particularly, research has increasingly explored the incorporation of resistant starch (RS) in dietary applications, as it does not add to calorie intake and helps regulate blood sugar levels, prevent constipation, and increase fecal volume, similar to dietary fiber. This study evaluated the quality characteristics and digestibility of malic acid-treated wheat starch (MA starch) with a high RS content for use as a rice flour substitute in <i>Sulgidduk</i>. MA starch was incorporated at 10-40% of the rice flour weight. Adding MA starch to <i>Sulgidduk</i> resulted in slight color changes, with decreased lightness and increased redness and yellowness, although structurally it remained unchanged. Higher MA levels reduced the pH and moisture content but increased sugar content and hardness. Compared to the control sample (rapidly digestible starch (RDS): 68.3%, RS: 30.3%), <i>Sulgidduk</i> prepared with MA starch had lower RDS (55.7%) and higher RS (42.3%) content. MA starch significantly affected RDS and RS levels, which are associated with the glycemic index, while also affecting moisture content and textural characteristics. Based on these findings, 30% substitution with MA starch (MA-30) appears to be the optimal ratio for maintaining health benefits while minimizing quality degradation. Collectively, these results highlight the potential of MA starch as a functional food ingredient and support its application in the formulation of dietary products for calorie management.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503037"},"PeriodicalIF":2.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Biohydrogen Production through Dark Fermentation by Humic Acid: Insights into Microbial Composition and Functional Genes.","authors":"Liguo Zhang, Yanan Bai, Jing Sang, Jinru Dong, Xiujuan Wu, Qiaoying Ban","doi":"10.4014/jmb.2412.12071","DOIUrl":"10.4014/jmb.2412.12071","url":null,"abstract":"<p><p>Biohydrogen production from organic waste or wastewater by eco-friendly methods has attracted attention in recent years. However, the biohydrogen yield still far below the theoretical value. In this study, humic acid (HA), a solid redox mediator (RM), was used to enhance the biohydrogen production from glucose. The internal mechanism based on microbial community and functional genes were explored. The results showed that the optimal dosages of HA were 80 and 150 mg/l with the biohydrogen yield of 312.7 and 315.5 ml/g glucose, which was higher than that in control by above 26.6%. A similar pattern of volatile fatty acids (VFAs) could be observed in all fermentation systems. Ethanol, acetate and propionate were the dominant by-products in all fermentation systems during the biohydrogen production process. The acetate concentration was significantly improved by adding 80 mg/l of HA. Microbial composition indicated that <i>Thermomarinilinea</i> was the most dominant bacterial genus in the fermentation systems containing HA. Compared with control, its relative abundance was increased by 1.0-fold~3.9-fold. However, redundancy analysis (RDA) indicated biohydrogen yield was closely correlated with <i>Gimesia</i>, <i>Longilinea</i>, <i>Defluviimonas</i>, <i>Pirellula</i> and <i>Planctomicrobium</i>. The functional genes based on KEGG pathways showed that most biohydrogen-producing related genes had not been significantly increased in the optimal dosage of HA systems compared with that in control, indicating that biohydrogen production was enhanced by HA might depend on accelerating electron transfer and adjusting microbial community in this study.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412071"},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Correlation between Gut Microbiota and Renal Function in Chronic Kidney Disease Patients.","authors":"Jun Xi, Jiru Xu, Jujun Sun, Huan Li, Siruo Zhang, He Xie, Haining Wang, Rui Zhang, Meng Zhao, Mi Wang, Tian Zheng","doi":"10.4014/jmb.2502.02039","DOIUrl":"10.4014/jmb.2502.02039","url":null,"abstract":"<p><p>Chronic kidney disease (CKD) has recently emerged as a significant, global public health threat, and the gut microbiota are now recognized as playing a crucial role in the pathogenesis and progression of CKD. In this study, we investigated the correlation between differential gut microbiota and renal function impairment by analyzing the structure, diversity, and other characteristics of the gut microbiota in patients with CKD. Our findings indicated that CKD patients exhibit decreased species diversity and evenness in their gut microbiota compared to healthy individuals, with notable differences in community composition between the groups. Among them, p_<i>Actinobacteriota</i>, p_Cyanobacteria, g_<i>Faecalibacterium</i>, g_<i>Agathobacter</i>, g_<i>Roseburia</i>, and g_<i>Actinomyces</i> were significantly decreased (<i>p</i> < 0.05), while p_Acidobacteriota, g_<i>Blautia</i>, and g_<i>Candidatus-Solibacter</i> were significantly increased in CKD (<i>p</i> < 0.05). Furthermore, functional prediction results suggested that the differential pathways primarily involved metabolic pathways, including Carbohydrate Metabolism, Glycan Biosynthesis and Metabolism, Biosynthesis of Other Secondary Metabolites, Metabolism of Other Amino Acids, and pathways related to Endocrine and Metabolic Diseases. Meanwhile, correlation studies revealed a significant negative correlation between g_<i>Actinomyces</i> and serum uric acid levels (<i>r</i> = -0.426, <i>p</i> = 0.038), and a significant positive correlation between g_<i>C. Solibacter</i> and serum uric acid levels (<i>r</i> = 0.461, <i>p</i> = 0.023). This study highlights the significant differences in the composition and species abundance of gut microbiota between CKD patients and healthy individuals, while also demonstrating that the abundances of g_<i>Actinomyces</i> and g_<i>C. Solibacter</i> are correlated with serum uric acid levels, an indicator of renal function impairment.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502039"},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and Evaluation of <i>Lactobacillus plantarum</i> LC5.2 Isolated from Thai Native Pigs for its Probiotic Potential in Gut Microbiota Modulation and Immune Enhancement.","authors":"Kittiya Khongkool, Malai Taweechotipatr, Sunchai Payungporn, Vorthon Sawaswong, Monthon Lertworapreecha","doi":"10.4014/jmb.2503.03028","DOIUrl":"10.4014/jmb.2503.03028","url":null,"abstract":"<p><p>Probiotic supplementation, particularly with <i>Lactobacillus</i> species, enhances growth performance, maintains gastrointestinal microbial balance, and prevents infections in livestock. This study isolated <i>Lactobacillus</i> strains from the feces of healthy native pigs in southern Thailand and assessed their probiotic properties and safety through both <i>in vitro</i> and <i>in vivo</i> evaluations. Nine <i>Lactobacillus</i> strains showed probiotic potential, with <i>Lactobacillus plantarum</i> LC5.2 demonstrating the best characteristics. This strain tolerated both acid and bile (100% tolerance) and exhibited strong adhesion properties, including high auto-aggregation (69.74%), cell surface hydrophobicity (77.14%), adhesion to Caco-2 cells (9.31%), and biofilm formation. It also exhibited antibacterial activity, inhibiting EHEC, EPEC, and <i>Salmonella</i> Typhimurium through organic acid production. Co-aggregation with these pathogens ranged from 60.83% to 74.09%. Safety evaluations showed no hemolytic activity, susceptibility to antibiotics, and co-existence with other probiotics. In mice, <i>L. plantarum</i> LC5.2 showed no toxicity, with normal food intake, behavior, and weight gain. No abnormalities were found in the small intestine, colon, liver, or spleen. Mice administered the probiotic had significantly higher intestinal IgA levels. Gut microbiome analysis revealed no notable structural alterations but indicated an increase in beneficial bacteria, including <i>Lactobacillus</i>. These results suggest that <i>L. plantarum</i> LC5.2 demonstrates strong probiotic potential, safety, and benefits for gut health, making it a promising candidate for livestock applications.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503028"},"PeriodicalIF":2.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}