{"title":"Diverse <i>Mycena</i> Fungi and Their Potential for <i>Gastrodia elata</i> Germination.","authors":"Xiao-Han Jin, Yu-Chuan Wang, Dong Li, Yu Li, Hai-Yan He, Han-Bo Zhang","doi":"10.4014/jmb.2401.01009","DOIUrl":"10.4014/jmb.2401.01009","url":null,"abstract":"<p><p>It remains to be determined whether there is a geographical distribution pattern and phylogenetic signals for the <i>Mycena</i> strains with seed germination of the orchid plant <i>Gastrodia elata</i>. This study analyzed the community composition and phylogenetics of 72 <i>Mycena</i> strains associated with <i>G. elata</i> varieties (<i>G. elata</i>. f. <i>glauca</i> and <i>G. elata</i>. f. <i>viridis</i>) using multiple gene fragments (ITS+nLSU+SSU). We found that (1) these diverse <i>Mycena</i> phylogenetically belong to the Basidiospore amyloid group. (2) There is a phylogenetic signal of <i>Mycena</i> for germination of <i>G. elata</i>. Those strains phylogenetically close to <i>M. abramsii</i>, <i>M. polygramma</i>, and an unclassified <i>Mycena</i> had significantly higher germination rates than those to <i>M. citrinomarginata</i>. (3) The <i>Mycena</i> distribution depends on geographic site and <i>G. elata</i> variety. Both unclassified <i>Mycena</i> group 1 and the <i>M. abramsii</i> group were dominant for the two varieties of <i>G. elata</i>; in contrast, the <i>M. citrinomarginata</i> group was dominant in <i>G. elata</i> f. <i>glauca</i> but absent in <i>G. elata</i> f. <i>viridis</i>. Our results indicate that the community composition of numerous <i>Mycena</i> resources in the Zhaotong area varies by geographical location and <i>G. elata</i> variety. Importantly, our results also indicate that <i>Mycena</i>'s phylogenetic status is correlated with its germination rate.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ye Won Jeon, Ha Min Song, Ka Yeong Lee, Yeong A Kim, Hyung Kwoun Kim
{"title":"Synthesis of Isoamyl Fatty Acid Ester, a Flavor Compound, by Immobilized <i>Rhodococcus</i> Cutinase.","authors":"Ye Won Jeon, Ha Min Song, Ka Yeong Lee, Yeong A Kim, Hyung Kwoun Kim","doi":"10.4014/jmb.2402.02033","DOIUrl":"10.4014/jmb.2402.02033","url":null,"abstract":"<p><p>Isoamyl fatty acid esters (IAFEs) are widely used as fruity flavor compounds in the food industry. In this study, various IAFEs were synthesized from isoamyl alcohol and various fatty acids using a cutinase enzyme (Rcut) derived from <i>Rhodococcus</i> bacteria. Rcut was immobilized on methacrylate divinylbenzene beads and used to synthesize isoamyl acetate, butyrate, hexanoate, octanoate, and decanoate. Among them, Rcut synthesized isoamyl butyrate (IAB) most efficiently. Docking model studies showed that butyric acid was the most suitable substrate in terms of binding energy and distance from the active site serine (Ser114) γ-oxygen. Up to 250 mM of IAB was synthesized by adjusting reaction conditions such as substrate concentration, reaction temperature, and reaction time. When the enzyme reaction was performed by reusing the immobilized enzyme, the enzyme activity was maintained at least six times. These results demonstrate that the immobilized Rcut enzyme can be used in the food industry to synthesize a variety of fruity flavor compounds, including IAB.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kyungha Lee, Man-Ho Cho, Mi-Ju Kim, Seong-Hee Bhoo
{"title":"A Small Epitope Tagging on the C-Terminus of a Target Protein Requires Extra Amino Acids to Enhance the Immune Responses of the Corresponding Antibody.","authors":"Kyungha Lee, Man-Ho Cho, Mi-Ju Kim, Seong-Hee Bhoo","doi":"10.4014/jmb.2401.01036","DOIUrl":"10.4014/jmb.2401.01036","url":null,"abstract":"<p><p>Protein-specific antibodies are essential for various aspects of protein research, including detection, purification, and characterization. When specific antibodies are unavailable, protein tagging is a useful alternative. Small epitope tags, typically less than 10 amino acids, are widely used in protein research due to the simple modification through PCR and reduced impact on the target protein's function compared to larger tags. The 2B8 epitope tag (RDPLPFFPP), reported by us in a previous study, has high specificity and sensitivity to the corresponding antibody. However, when attached to the C-terminus of the target protein in immunoprecipitation experiments, we observed a decrease in detection signal with reduced immunity and low protein recovery. This phenomenon was not unique to 2B8 and was also observed with the commercially available Myc tag. Our study revealed that C-terminal tagging of small epitope tags requires the addition of more than one extra amino acid to enhance (restore) antibody immunities. Moreover, among the amino acids we tested, serine was the best for the 2B8 tag. Our findings demonstrated that the interaction between a small epitope and a corresponding paratope of an antibody requires an extra amino acid at the C-terminus of the epitope. This result is important for researchers planning studies on target proteins using small epitope tags.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141087575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacterial Growth Modulatory Effects of Two Branched-Chain Hydroxy Acids and Their Production Level by Gut Microbiota.","authors":"Chan Hyuk Hwang, Su-Hyun Kim, Choong Hwan Lee","doi":"10.4014/jmb.2404.04009","DOIUrl":"10.4014/jmb.2404.04009","url":null,"abstract":"<p><p>Branched-chain hydroxy acids (BCHAs), produced by lactic acid bacteria, have recently been suggested as bioactive compounds contributing to the systemic metabolism and modulation of the gut microbiome. However, the relationship between BCHAs and gut microbiome remains unclear. In this study, we investigated the effects of BCHAs on the growth of seven different families in the gut microbiota. Based on in vitro screening, both 2-hydroxyisovaleric acid (HIVA) and 2-hydroxyisocaproic acid (HICA) stimulated the growth of <i>Lactobacillaceae</i> and <i>Bifidobacteriaceae</i>, with HIVA showing a significant growth promotion. Additionally, we observed not only the growth promotion of probiotic <i>Lactobacillaceae</i> strains but also growth inhibition of pathogenic <i>B. fragilis</i> in a dosedependent manner. The production of HIVA and HICA varied depending on the family of the gut microbiota and was relatively high in case of <i>Lactobacillaceae</i> and <i>Lachnosporaceae</i>. Furthermore, HIVA and HICA production by each strain positively correlated with their growth variation. These results demonstrated gut microbiota-derived BCHAs as active metabolites that have bacterial growth modulatory effects. We suggest that BCHAs can be utilized as active metabolites, potentially contributing to the treatment of diseases associated with gut dysbiosis.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Mesorhizobium koreense</i> sp. nov., Isolated from Soil.","authors":"Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim","doi":"10.4014/jmb.2404.04026","DOIUrl":"https://doi.org/10.4014/jmb.2404.04026","url":null,"abstract":"<p><p>An aerobic, Gram-stain-negative, catalase-positive, rod-shaped, and motile bacteria, designated as a strain WR6<sup>T</sup> was isolated from soil in Republic of Korea. Strain WR6<sup>T</sup> grew at temperatures of 10- 37°C, at pH of 5.0-9.0, and at NaCl concentrations of 0-3.0% (w/v). Phylogenetic and 16S rRNA gene nucleotide sequence analysis confirmed that strain WR6<sup>T</sup> affiliated to the genus <i>Mesorhizobium</i>, with the nearest relative being Mesorhizobium waimense ICMP 19557T (98.5%). The genome of strain WR6<sup>T</sup> was 5,035,462 bp with DNA G+C content of 62.6%. In strain WR6<sup>T</sup> , Q-10 was sole ubiquinone; summed feature 8 (C<sub>18:1</sub>ω7c and/or C<sub>18: 1</sub>ω6c) and C<sub>19:0</sub> cyclo <i>ω</i>8c were predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were major polar lipids. Based on these polyphasic taxonomic data, strain WR6<sup>T</sup> represents a novel species in the genus <i>Mesorhizobium.</i> Accordingly, we propose the name <i>Mesorhizobium koreense</i> sp. nov., with the type strain WR6<sup>T</sup> (=KCTC 92695<sup>T</sup> =NBRC 116021<sup>T</sup> ).</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anh Pham Thi Ngoc, Adil Zahoor, Dong Gyun Kim, Seung Hwan Yang
{"title":"Using Synbiotics as a Therapy to Protect Mental Health in Alzheimer's Disease.","authors":"Anh Pham Thi Ngoc, Adil Zahoor, Dong Gyun Kim, Seung Hwan Yang","doi":"10.4014/jmb.2403.03021","DOIUrl":"https://doi.org/10.4014/jmb.2403.03021","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a progressive neurological disorder that represents a major cause of dementia worldwide. Its pathogenesis involves multiple pathways, including the amyloid cascade, tau protein, oxidative stress, and metal ion dysregulation. Recent studies have suggested a critical link between changes in gut microbial diversity and the disruption of the gut-brain axis in AD. Previous studies primarily explored the potential benefits of probiotics and prebiotics in managing AD. However, studies have yet to fully describe a novel promising approach involving the use of synbiotics, which include a combination of active probiotics and new-generation prebiotics. Synbiotics show potential for mitigating the onset and progression of AD, thereby offering a holistic approach to address the multifaceted nature of AD. This review article primarily aims to gain further insights into the mechanisms of AD, specifically the intricate interaction between gut bacteria and the brain via the gut-brain axis. By understanding this relationship, we can identify potential targets for intervention and therapeutic strategies to combat AD effectively. This review also discusses substantial evidence supporting the role of synbiotics as a promising AD treatment that surpasses traditional probiotic or prebiotic interventions. We find that synbiotics may be used not only to address cognitive decline but also to reduce AD-related psychological burden, thus enhancing the overall quality of life of patients with AD.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huan Huan Yan, Yi Tong Shang, Li Hong Wang, Xue Qin Tian, Van-Tuan Tran, Li Hua Yao, Bin Zeng, Zhi Hong Hu
{"title":"Construction of a New <i>Agrobacterium tumefaciens</i>-Mediated Transformation System based on a Dual Auxotrophic Approach in <i>Cordyceps militaris</i>.","authors":"Huan Huan Yan, Yi Tong Shang, Li Hong Wang, Xue Qin Tian, Van-Tuan Tran, Li Hua Yao, Bin Zeng, Zhi Hong Hu","doi":"10.4014/jmb.2312.12003","DOIUrl":"10.4014/jmb.2312.12003","url":null,"abstract":"<p><p><i>Cordyceps militaris</i> is a significant edible fungus that produces a variety of bioactive compounds. We have previously established a uridine/uracil auxotrophic mutant and a corresponding <i>Agrobacterium tumefaciens</i>-mediated transformation (ATMT) system for genetic characterization in <i>C. militaris</i> using <i>pyrG</i> as a screening marker. In this study, we constructed an ATMT system based on a dual <i>pyrG</i> and <i>hisB</i> auxotrophic mutant of <i>C. militaris</i>. Using the uridine/uracil auxotrophic mutant as the background and <i>pyrG</i> as a selection marker, the <i>hisB</i> gene encoding imidazole glycerophosphate dehydratase, required for histidine biosynthesis, was knocked out by homologous recombination to construct a histidine auxotrophic <i>C. militaris</i> mutant. Then, <i>pyrG</i> in the histidine auxotrophic mutant was deleted to construct a Δ<i>pyrG</i> Δ<i>hisB</i> dual auxotrophic mutant. Further, we established an ATMT transformation system based on the dual auxotrophic <i>C. militaris</i> by using <i>GFP</i> and <i>DsRed</i> as reporter genes. Finally, to demonstrate the application of this dual transformation system for studies of gene function, knock out and complementation of the photoreceptor gene Cm<i>WC-1</i> in the dual auxotrophic <i>C. militaris</i> were performed. The newly constructed ATMT system with histidine and uridine/uracil auxotrophic markers provides a promising tool for genetic modifications in the medicinal fungus <i>C. militaris</i>.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniel Junpyo Lee, Ju Young Eor, Min-Jin Kwak, Junbeom Lee, An Na Kang, Daye Mun, Hyejin Choi, Minho Song, Jong Nam Kim, Jun-Mo Kim, Jungwoo Yang, Hyung Wook Kim, Sangnam Oh, Younghoon Kim
{"title":"Metabolic Regulation of Longevity and Immune Response in <i>Caenorhabditis elegans</i> by Ingestion of <i>Lacticaseibacillus rhamnosus</i> IDCC 3201 Using Multi-Omics Analysis.","authors":"Daniel Junpyo Lee, Ju Young Eor, Min-Jin Kwak, Junbeom Lee, An Na Kang, Daye Mun, Hyejin Choi, Minho Song, Jong Nam Kim, Jun-Mo Kim, Jungwoo Yang, Hyung Wook Kim, Sangnam Oh, Younghoon Kim","doi":"10.4014/jmb.2402.02025","DOIUrl":"10.4014/jmb.2402.02025","url":null,"abstract":"<p><p>Probiotics, specifically <i>Lacticaseibacillus rhamnosus</i>, have garnered attention for their potential health benefits. This study focuses on evaluating the probiotic properties of candidate probiotics <i>L. rhamnosus</i> IDCC 3201 (3201) using the <i>Caenorhabditis elegans</i> surrogate animal model, a well-established <i>in vivo</i> system for studying host-bacteria interactions. The adhesive ability to the host's gastrointestinal tract is a crucial criterion for selecting potential probiotic bacteria. Our findings demonstrated that 3201 exhibits significantly higher adhesive capabilities compared with <i>Escherichia coli</i> OP50 (OP50), a standard laboratory food source for <i>C. elegans</i> and is comparable with the widely recognized probiotic <i>L. rhamnosus</i> GG (LGG). In lifespan assay, 3201 significantly increased the longevity of <i>C. elegans</i> compared with OP50. In addition, preconditioning with 3201 enhanced <i>C. elegans</i> immune response against four different foodborne pathogenic bacteria. To uncover the molecular basis of these effects, transcriptome analysis elucidated that 3201 modulates specific gene expression related to the innate immune response in <i>C. elegans</i>. C-type lectin-related genes and lysozyme-related genes, crucial components of the immune system, showed significant upregulation after feeding 3201 compared with OP50. These results suggested that preconditioning with 3201 may enhance the immune response against pathogens. Metabolome analysis revealed increased levels of fumaric acid and succinic acid, metabolites of the citric acid cycle, in <i>C. elegans</i> fed with 3201 compared with OP50. Furthermore, there was an increase in the levels of lactic acid, a well-known antimicrobial compound. This rise in lactic acid levels may have contributed to the robust defense mechanisms against pathogens. In conclusion, this study demonstrated the probiotic properties of the candidate probiotic <i>L. rhamnosus</i> IDCC 3201 by using multi-omics analysis.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minju Jung, YouKyeong Lee, Sung Ok Han, Jeong Eun Hyeon
{"title":"Advancements in Sustainable Plant-Based Alternatives: Exploring Proteins, Fats, and Manufacturing Challenges in Alternative Meat Production.","authors":"Minju Jung, YouKyeong Lee, Sung Ok Han, Jeong Eun Hyeon","doi":"10.4014/jmb.2312.12049","DOIUrl":"10.4014/jmb.2312.12049","url":null,"abstract":"<p><p>The rise in plant-based food consumption is propelled by concerns for sustainability, personal beliefs, and a focus on healthy dietary habits. This trend, particularly in alternative meat, has attracted attention from specialized brands and eco-friendly food companies, leading to increased interest in plant-based alternatives. The dominant plant-based proteins, derived mainly from legumes, include soy protein isolates, which significantly impact sensory factors. In the realm of plant-based fats, substitutes are categorized into fat substitutes based on fats and fat mimetics based on proteins and carbohydrates. The production of these fats, utilizing gums, emulsions, gels, and additives, explores characteristics influencing the appearance, texture, flavor, and storage stability of final plant-based products. Analysis of plant-based proteins and fats in hamburger patties provides insights into manufacturing methods and raw materials used by leading alternative meat companies. However, challenges persist, such as replicating meat's marbling characteristic and addressing safety considerations in terms of potential allergy induction and nutritional supplementation. To enhance functionality and develop customized plant-based foods, it is essential to explore optimal combinations of various raw materials and develop new plant-based proteins and fat separation.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ju Hee Lee, Hye Jin Jeon, Sangsu Seo, Chorong Lee, Bumkeun Kim, Dong-Mi Kwak, Man Hee Rhee, Patharapol Piamsomboon, Yani Lestari Nuraini, Chang Uook Je, Seon Young Park, Ji Hyung Kim, Jee Eun Han
{"title":"The Use of the Internal Transcribed Spacer Region for Phylogenetic Analysis of the Microsporidian Parasite <i>Enterocytozoon hepatopenaei</i> Infecting Whiteleg Shrimp (<i>Penaeus vannamei</i>) and for the Development of a Nested PCR as Its Diagnostic Tool.","authors":"Ju Hee Lee, Hye Jin Jeon, Sangsu Seo, Chorong Lee, Bumkeun Kim, Dong-Mi Kwak, Man Hee Rhee, Patharapol Piamsomboon, Yani Lestari Nuraini, Chang Uook Je, Seon Young Park, Ji Hyung Kim, Jee Eun Han","doi":"10.4014/jmb.2401.01010","DOIUrl":"10.4014/jmb.2401.01010","url":null,"abstract":"<p><p>The increasing economic losses associated with growth retardation caused by <i>Enterocytozoon hepatopenaei</i> (EHP), a microsporidian parasite infecting penaeid shrimp, require effective monitoring. The internal transcribed spacer (ITS)-1 region, the non-coding region of ribosomal clusters between 18S and 5.8S rRNA genes, is widely used in phylogenetic studies due to its high variability. In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (<i>Penaeus vannamei</i> and <i>P. monodon</i>) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) genes. Phylogenetic analysis based on the ITS-1 sequences indicated that the Indonesian strain was distinct (86.2% nucleotide sequence identity) from other strains collected from Thailand and South Korea, and also showed the internal diversity among Thailand (<i>N</i> = 7, divided into four branches) and South Korean (<i>N</i> = 5, divided into two branches) samples. The results revealed the ability of the ITS-1 region to determine the genetic diversity of EHP from different geographical origins.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}