Journal of microbiology and biotechnology最新文献_第10页

Effects of Exocellobiohydrolase CBHA on Fermentation of Tobacco Leaves. 外细胞生物水解酶 CBHA 对烟叶发酵的影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-19 DOI: 10.4014/jmb.2404.04028

Xueqin Xu, Qianqian Wang, Longyan Yang, Zhiyan Chen, Yun Zhou, Hui Feng, Peng Zhang, Jie Wang

{"title":"Effects of Exocellobiohydrolase CBHA on Fermentation of Tobacco Leaves.","authors":"Xueqin Xu, Qianqian Wang, Longyan Yang, Zhiyan Chen, Yun Zhou, Hui Feng, Peng Zhang, Jie Wang","doi":"10.4014/jmb.2404.04028","DOIUrl":"10.4014/jmb.2404.04028","url":null,"abstract":"The quality of tobacco is directly affected by macromolecular content, fermentation is an effective method to improve biochemical properties. In this study, we utilized CBHA (cellobiohydrolase A) glycosylase, which was expressed by Pichia pastoris, as an additive for fermentation. The contents of main chemical components of tobacco leaves after fermentation were determined, and the changes of microbial community structure and abundance in tobacco leaves during fermentation were analyzed. The relationship between chemical composition and changes in microbial composition was investigated, and the function of bacteria and fungi in fermentation was predicted to identify possible metabolic pathways. After 48 h of CBHA fermentation, the contents of starch, cellulose and total nitrogen in tobacco leaf decreased by 17.60%, 28.91% and 16.05%, respectively. The microbial community structure changed significantly, with Aspergillus abundance decreasing significantly, while Filobasidum, Cladosporium, Bullera, Komagataella, etc., increased in CBHA treated group. Soluble sugar was most affected by microbial community in tobacco leaves, which was negatively correlated with starch, cellulose and total nitrogen. During the fermentation process, the relative abundance of metabolism-related functional genes increased, and the expressions of cellulase and endopeptidase also increased. The results showed that the changes of bacterial community and dominant microbial community on tobacco leaves affected the content of chemical components in tobacco leaves, and adding CBHA for fermentation had a positive effect on improving the quality of tobacco leaves.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1727-1737"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Truncated CHAP Modular Endolysin, CHAP^SAP26-161, That Lyses Staphylococcus aureus, Acinetobacter baumannii, and Clostridioides difficile, and Exhibits Therapeutic Effects in a Mouse Model of A. baumannii Infection. 一种新型截短 CHAP 模块化内溶酶 CHAPSAP26-161，可溶解金黄色葡萄球菌、鲍曼不动杆菌和艰难梭菌，并在鲍曼不动杆菌感染小鼠模型中显示出治疗效果。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-17 DOI: 10.4014/jmb.2402.02042

Yoon-Jung Choi, Shukho Kim, Ram Hari Dahal, Jungmin Kim

{"title":"A Novel Truncated CHAP Modular Endolysin, CHAPSAP26-161, That Lyses Staphylococcus aureus, Acinetobacter baumannii, and Clostridioides difficile, and Exhibits Therapeutic Effects in a Mouse Model of A. baumannii Infection.","authors":"Yoon-Jung Choi, Shukho Kim, Ram Hari Dahal, Jungmin Kim","doi":"10.4014/jmb.2402.02042","DOIUrl":"10.4014/jmb.2402.02042","url":null,"abstract":"Development of novel antibacterial agents is imperative due to the increasing threat of antibiotic-resistant pathogens. This study aimed to develop the enhanced antibacterial activity and in-vivo efficacy of a novel truncated endolysin, CHAPSAP26-161, derived from the endolysin LysSAP26, against multidrug-resistant bacteria. CHAPSAP26-161 exhibited higher protein purification efficiency in E. coli and antibacterial activity than LysSAP26. Moreover, CHAPSAP26-161 showed the higher lytic activity against A. baumannii with minimal bactericidal concentrations (MBCs) of 5-10 μg/ml, followed by Staphylococcus aureus with MBCs of 10-25 μg/ml. Interestingly, CHAPSAP26-161 could lyse anaerobic bacteria, such as Clostridioides difficile, with MBCs of 25-50 μg/ml. At pH 4-8 and temperatures of 4°C-45°C, CHAPSAP26-161 maintained antibacterial activity without remarkable difference. The lytic activity of CHAPSAP26-161 was increased with Zn2+. In vivo tests demonstrated the therapeutic effects of CHAPSAP26-161 in murine systemic A. baumannii infection model. In conclusion, CHAPSAP26-161, a truncated endolysin that retains only the CHAP domain from LysSAP26, demonstrated enhanced protein purification efficiency and antibacterial activity compared to LysSAP26. It further displayed broad-spectrum antibacterial effects against S. aureus, A. baumannii, and C. difficile. Our in vitro and in-vivo results of CHAPSAP26-161 highlights its promise as an innovative therapeutic option against those bacteria with multiple antibiotic resistance.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1718-1726"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Levansucrase Operon Regulating Levan-Type Fructooligosaccharides Production in Priestia koreensis HL12. 探索调控朝鲜芒柄伞 HL12 中左旋果寡糖生产的左旋琥珀酸酶操作子

IF 2.8 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-23 DOI: 10.4014/jmb.2404.04043

Hataikarn Lekakarn,Daran Prongjit,Wuttichai Mhuantong,Srisakul Trakarnpaiboon,Benjarat Bunterngsook

{"title":"Exploring Levansucrase Operon Regulating Levan-Type Fructooligosaccharides Production in Priestia koreensis HL12.","authors":"Hataikarn Lekakarn,Daran Prongjit,Wuttichai Mhuantong,Srisakul Trakarnpaiboon,Benjarat Bunterngsook","doi":"10.4014/jmb.2404.04043","DOIUrl":"https://doi.org/10.4014/jmb.2404.04043","url":null,"abstract":"Levan biopolymer and levan-type fructooligosaccharides (L-FOSs) are β-2,6-linked fructans that have been used as non-digestible dietary fiber and prebiotic oligosaccharides in food and cosmeceutical applications. In this study, we explore the operon responsible for levan and lL-FOSs production in Priestia koreensis HL12. Presented is the first genomic perspective on sucrose utilization and the levan biosynthesis pathway in this bacterium. Regarding sequence annotation, the putative levansucrase operon responsible for β-2,6-linked fructan was identified in the genome of strain HL12, and comprises sacB levansucrase gene belonging to GH68, located adjacent to levB endo-levanase gene, which belongs to GH32. Importantly, sugars related with the levan biosynthesis pathway are proposed to be transported via 3 types of transportation systems, including multiple ABCSugar and glucose/H+ transporters, as well as glucoseand fructose-specific PTS systems. Based on product profile analysis, the HL12 strain exhibited high efficiency in levan production from high sucrose concentration (300 g/l), achieving the highest yield of 127 g/l (equivalent to 55% conversion based on sucrose consumption), together with short-chain L-FOSs (DP3-5) and long-chain L-FOSs with respective size larger than DP6 after 48 h incubation. These findings highlight the potential of P. koreensis HL12 as a whole-cell biocatalyst for producing levan and L-FOSs, and underscore its novelty in converting sugars into high-value-added products for diverse commercial and industrial applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"6 1","pages":"1-10"},"PeriodicalIF":2.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limosilactobacillus fermentum SLAM 216- Derived Extracellular Vesicles Promote Intestinal Maturation in Mouse Organoid Models. 发酵柠檬酸乳杆菌 SLAM 216 衍生的细胞外小泡可促进小鼠类器官模型的肠道成熟。

IF 2.8 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-09 DOI: 10.4014/jmb.2405.05028

Hyejin Choi,Min-Jin Kwak,An Na Kang,Daye Mun,Suengwon Lee,Mi Ri Park,Sangnam Oh,Younghoon Kim

{"title":"Limosilactobacillus fermentum SLAM 216- Derived Extracellular Vesicles Promote Intestinal Maturation in Mouse Organoid Models.","authors":"Hyejin Choi,Min-Jin Kwak,An Na Kang,Daye Mun,Suengwon Lee,Mi Ri Park,Sangnam Oh,Younghoon Kim","doi":"10.4014/jmb.2405.05028","DOIUrl":"https://doi.org/10.4014/jmb.2405.05028","url":null,"abstract":"Probiotics, when consumed in adequate amounts, can promote the health of the host and beneficially modulate the host's immunity. Particularly during the host's early life, the gut intestine undergoes a period of epithelial maturation in which epithelial cells organize into specific crypt and villus structures. This process can be mediated by the gut microbiota. Recent studies have reported that the administration of probiotics can further promote intestinal maturation in the neonatal intestine. Therefore, in this study, we investigated the effects of extracellular vesicles derived from the Limosilactobacillus fermentum SLAM 216 strain, which is an established probiotic with known immune and anti-aging effects on intestinal epithelial maturation and homeostasis, using mouse small intestinal organoids. As per our findings, treatment with L. fermentum SLAM 216-derived LF216EV (LF216EV) has significantly increased the bud number and size of organoid buds. Furthermore, extracellular vesicle (EV) treatment upregulated the expression of maturation-related genes, including Ascl2, Ephb2, Lgr5, and Sox9. Tight junctions are known to have an important role in the intestinal immune barrier, and EV treatment has significantly increased the expression of genes associated with tight junctions, such as Claudin, Muc2, Occludin, and Zo-1, indicating that it can promote intestinal development. This was supported by RNA sequencing, which revealed the upregulation of genes associated with cAMP-mediated signaling, which is known to regulate cellular processes including cell differentiation. Additionally, organoids exposed to LF216EV exhibited upregulation of genes associated with maintaining brain memory and neurotransmission, suggesting possible future functional implications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"14 1","pages":"1-9"},"PeriodicalIF":2.8,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine Learning Using Template-BasedPredicted Structure of Haemagglutinin Predicts Pathogenicity of Avian Influenza. 使用基于模板的血凝素预测结构的机器学习可预测禽流感的致病性。

IF 2.8 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-06 DOI: 10.4014/jmb.2405.05022

Jong Hyun Shin,Sun Ju Kim,Gwanghun Kim,Hang-Rae Kim,Kwan Soo Ko

{"title":"Machine Learning Using Template-BasedPredicted Structure of Haemagglutinin Predicts Pathogenicity of Avian Influenza.","authors":"Jong Hyun Shin,Sun Ju Kim,Gwanghun Kim,Hang-Rae Kim,Kwan Soo Ko","doi":"10.4014/jmb.2405.05022","DOIUrl":"https://doi.org/10.4014/jmb.2405.05022","url":null,"abstract":"Deep learning presents a promising approach to complex biological classifications, contingent upon the availability of well-curated datasets. This study addresses the challenge of analyzing threedimensional protein structures by introducing a novel pipeline that utilizes open-source tools to convert protein structures into a format amenable to computational analysis. Applying a twodimensional convolutional neural network (CNN) to a dataset of 12,143 avian influenza virus genomes from 64 countries, encompassing 119 hemagglutinin (HA) and neuraminidase (NA) types, we achieved significant classification accuracy. The pathogenicity was determined based on the presence of H5 or H7 subtypes, and our models, ranging from zero to six mid-layers, indicated that a four-layer model most effectively identified highly pathogenic strains, with accuracies over 0.9. Enhancing our approach, we incorporated Principal Component Analysis (PCA) for dimensionality reduction and one-class SVM for abnormality detection, improving model robustness through bootstrapping. Furthermore, the K-nearest neighbor (K-NN) algorithm was fine-tuned via hyperparameter optimization to corroborate the findings. The PCA identified distinct clustering for pathogenic HA, yielding an AUC of up to 0.85. The optimized K-NN model demonstrated an impressive accuracy between 0.96 and 0.97. These combined methodologies underscore our deep learning framework's capacity for rapid and precise identification of pathogenic avian influenza strains, thus providing a critical tool for managing global avian influenza threats.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"11 1","pages":"1-8"},"PeriodicalIF":2.8,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of Active Compound of Terminalia catappa L. Extract and Its AntiInflammatory and Antioxidant Properties by Regulating Mitochondrial Dysfunction and Cellular Signaling Pathways. 通过调节线粒体功能障碍和细胞信号通路，验证 Terminalia catappa L. 提取物的活性化合物及其抗炎和抗氧化特性

IF 2.8 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-02 DOI: 10.4014/jmb.2407.07044

So Jeong Paik,Dong-Shin Kim,Joe Eun Son,Tran The Bach,Do Van Hai,Jin-Hyub Paik,Sangjin Jo,Dong Joon Kim,Sung Keun Jung

{"title":"Validation of Active Compound of Terminalia catappa L. Extract and Its AntiInflammatory and Antioxidant Properties by Regulating Mitochondrial Dysfunction and Cellular Signaling Pathways.","authors":"So Jeong Paik,Dong-Shin Kim,Joe Eun Son,Tran The Bach,Do Van Hai,Jin-Hyub Paik,Sangjin Jo,Dong Joon Kim,Sung Keun Jung","doi":"10.4014/jmb.2407.07044","DOIUrl":"https://doi.org/10.4014/jmb.2407.07044","url":null,"abstract":"As chronic inflammation and oxidative stress cause various diseases in the human body, this study aimed to develop functional materials to prevent inflammation and oxidative stress. This study investigated the biological function and components of Terminalia catappa L. extract prepared using its leaves and branches (TCE). TCE was determined using ultraperformance liquid chromatographyquadrupole-time-of-flight mass spectrometry. Using RAW 264.7 mouse macrophages, inhibitory effects of the identified compounds on nitric oxide (NO) and reactive oxygen species (ROS) generation were analyzed. Therefore, α-punicalagin was selected as an active compound with the highest content (986.6 ± 68.4 μg/g) and physiological activity. TCE exhibited an inhibitory effect on lipopolysaccharide (LPS)-induced inflammatory markers, including NO, inducible nitric oxide synthase, and inflammatory cytokines without exerting cytotoxicity. Moreover, TCE prevented excessive ROS production mediated by LPS and upregulated hemeoxygenase-1 expression via the nuclear translocation of nuclear factor erythroid 2-related factor 2. Interestingly, TCE prevented LPS-induced mitochondrial membrane potential loss, mitochondrial ROS production, and dynaminrelated protein 1 phosphorylation (serine 616), a marker of abnormal mitochondrial fission. Furthermore, TCE considerably repressed the activation of LPS-induced mitogen-activated protein kinase pathway. Thus, TCE is a promising anti-inflammatory and antioxidant pharmaceutical or nutraceutical, as demonstrated via mitochondrial dysfunction and cellular signaling pathway regulation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"6 1","pages":"1-14"},"PeriodicalIF":2.8,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Sequencing of Three Lactiplantibacillus plantarum Strains Reveals Potential Metabolites for Boosting Host Immunity Safely. 三个植物乳杆菌菌株的全基因组测序揭示了安全提高宿主免疫力的潜在代谢物。

IF 2.8 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-07-31 DOI: 10.4014/jmb.2402.02013

I-Chen Li,Yueh-Lun Lee,Tsung-Ju Li,You-Shan Tsai,Yen-Lien Chen,Chin-Chu Chen

{"title":"Whole-Genome Sequencing of Three Lactiplantibacillus plantarum Strains Reveals Potential Metabolites for Boosting Host Immunity Safely.","authors":"I-Chen Li,Yueh-Lun Lee,Tsung-Ju Li,You-Shan Tsai,Yen-Lien Chen,Chin-Chu Chen","doi":"10.4014/jmb.2402.02013","DOIUrl":"https://doi.org/10.4014/jmb.2402.02013","url":null,"abstract":"In response to the growing demand for immune-related products, this study evaluated the safety and immune-modulating potential of three newly discovered Lactiplantibacillus plantarum strains (GKM3, GKK1, and GKD7) through toxicity tests and whole-genome sequencing. Safety evaluations, including the analysis of antimicrobial resistance genes, virulence factors, plasmids, and prophages, classified these strains as safe for human consumption. Acute oral toxicity tests further supported their safety. To evaluate their immune-modulating potential, dendritic cells were exposed to these strains, and the secretion of key cytokines (IFN-β and IL-12) was measured. Among the strains, GKK1 exhibited the highest enhancement of IFN-β and IL-12 production, suggesting its potential as an immune-stimulating probiotic. Bioinformatics analysis revealed potential metabolic pathways and secondary metabolites, including predicted bacteriocins, associated with immune modulation. The presence of a nitrate reductase region in the GKK1 strain indicated its ability to produce nitric oxide, a critical molecule involved in immune regulation and host defense. The presence of glucorhamnanrelated gene clusters in GKK1 also suggested immune-enhancing effects. Nitrate reductase expression was confirmed using qPCR, with the highest levels detected in GKK1. Moreover, this study is the first to show an anti-inflammatory effect of plantaricin A, linked to its presence in strain GKM3 and its potential therapeutic applications due to sequence similarity to known antiinflammatory peptides. Overall, these three L. plantarum strains demonstrated a safe profile and GKK1 showed potential as an immunity-enhancing probiotic. However, additional investigation is required to confirm the involvement of specific metabolic pathways, secondary metabolites, and bacteriocins in immune responses.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"24 1","pages":"1-10"},"PeriodicalIF":2.8,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Overview of Candida auris: An Emerging Multidrug-Resistant Fungal Pathogen. 全面概述白色念珠菌：一种新出现的耐多药真菌病原体。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-07-28 Epub Date: 2024-06-15 DOI: 10.4014/jmb.2404.04040

Ji-Seok Kim, Hyunjin Cha, Yong-Sun Bahn

引用次数: 0

HPLC, NMR Based Characterization, Antioxidant and Anticancer Activities of Chemical Constituents from Therapeutically Active Fungal Endophytes. 具有治疗活性的真菌内生菌中化学成分的高效液相色谱和核磁共振表征、抗氧化和抗癌活性。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-07-28 Epub Date: 2024-05-06 DOI: 10.4014/jmb.2403.03036

Waqas Hussain Shah, Wajiha Khan, Sobia Nisa, Michael H J Barfuss, Johann Schinnerl, Markus Bacher, Karin Valant-Vetschera, Ashraf Ali, Hiba-Allah Nafidi, Yousef A Bin Jardan, John P Giesy

{"title":"HPLC, NMR Based Characterization, Antioxidant and Anticancer Activities of Chemical Constituents from Therapeutically Active Fungal Endophytes.","authors":"Waqas Hussain Shah, Wajiha Khan, Sobia Nisa, Michael H J Barfuss, Johann Schinnerl, Markus Bacher, Karin Valant-Vetschera, Ashraf Ali, Hiba-Allah Nafidi, Yousef A Bin Jardan, John P Giesy","doi":"10.4014/jmb.2403.03036","DOIUrl":"10.4014/jmb.2403.03036","url":null,"abstract":"Fungi generate different metabolites some of which are intrinsically bioactive and could therefore serve as templates for drug development. In the current study, six endophytic fungi namely Aspergillus flavus, Aspergillus tubigenesis, Aspergillus oryzae, Penicillium oxalicum, Aspergillus niger, and Aspergillus brasiliensis were isolated and identified from the medicinal plant, Silybum marianum. These endophytic fungi were identified through intra transcribed sequence (ITS) gene sequencing. The bioactive potentials of fungal extracts were investigated using several bioassays such as antibacterial activity by well-diffusion, MIC, MBC, anti-biofilm, antioxidant, and haemolysis. The Pseudomonas aeruginosa PAO1 was used to determine the antibiofilm activity. The ethyl acetate extract of Aspergillus flavus showed strong to moderate efficacy against Staphylococcus aureus, Escherichia coli, P. aeruginosa, and Bacillus spizizenii. Aspergillus flavus and Aspergillus brasiliensis exhibited significant antibiofilm activity with IC50 at 4.02 and 3.63 mg/ml, while A. flavus exhibited maximum antioxidant activity of 50.8%. Based on HPLC, LC-MS, and NMR experiments kojic acid (1) and carbamic acid (methylene-4, 1-phenylene) bis-dimethyl ester (2) were identified from A. flavus. Kojic acid exhibited DPPH free radical scavenging activity with an IC50 value of 99.3 μg/ml and moderate activity against ovarian teratocarcinoma (CH1), colon carcinoma (SW480), and non-small cell lung cancer (A549) cell lines. These findings suggest that endophytic fungi are able to produce promising bioactive compounds which deserve further investigation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 7","pages":"1452-1463"},"PeriodicalIF":2.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11294646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141300848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Apoptosis-Like Death through Accumulation of Reactive Oxygen Species by Quercetin in Escherichia coli. 槲皮素在大肠杆菌中通过积累活性氧导致细菌凋亡式死亡

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-07-28 Epub Date: 2024-06-14 DOI: 10.4014/jmb.2403.03057

Min Seok Kwun, Dong Gun Lee

{"title":"Bacterial Apoptosis-Like Death through Accumulation of Reactive Oxygen Species by Quercetin in Escherichia coli.","authors":"Min Seok Kwun, Dong Gun Lee","doi":"10.4014/jmb.2403.03057","DOIUrl":"10.4014/jmb.2403.03057","url":null,"abstract":"The antimicrobial activity of the natural compounds from plant and food have well discovered since the interest on the beneficial effect of the natural compounds was risen. Quercetin, a flavonoid derived from vegetables, including onions, red leaf lettuces and cherries has been studied for diverse biological characteristics as anti-cancer and anti-microbial activities. The aim of current study is to investigate the specific antibacterial modes of action of quercetin against Escherichia coli. Quercetin decreased the E. coli cell viability and induced the severe damages (oxidative stress, DNA fragmentation) leading to cell death. Reactive oxygen species (ROS) generation was observed during the process, which we confirmed that oxidative stress was the key action of antibacterial activity of quercetin exerting its influence potently. Based on the results of Annexin V and Caspace FITC-VAD-FMK assay, the oxidative damage in E. coli has led to the bacterial apoptosis-like death in E. coli. To sum up, the contribution of ROS generation exerts crucial impact in antibacterial activity of quercetin.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 7","pages":"1395-1400"},"PeriodicalIF":2.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11294654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0