Journal of microbiology and biotechnology最新文献

{"title":"Comparative Analysis of Signature Sequences from Adenylation Domains Situated within Bacterial-Origin Nonribosomal Peptide Synthetase Modules.","authors":"Weina Gao, Zhishen Zhang, Huiying Yu, Xin Li, Chunshan Quan, Yun Xue, Pengchao Zhao","doi":"10.4014/jmb.2503.02030","DOIUrl":"10.4014/jmb.2503.02030","url":null,"abstract":"<p><p>Nonribosomal peptides are assembled by large enzymes that contain multiple active sites, which function in a modular manner. The adenylation (A) domains present within typical nonribosomal peptide synthetase (NRPS) modules contain specificity-conferring codes or signature sequences (SNSs). In this study, we obtained 2051 A domain sequences from 67 bacterial species. Their alignment and clustering identified 508 SNSs. Over 80% of the SNSs displayed distinct specificity for 36 proteinogenic and nonproteinogenic α-amino acid moieties (α-AAMs). Furthermore, modifications such as <i>N</i>-methylation, monooxygenase activity, and oxidation contributed to the elongation of the A domains, while conferring pronounced affinities for certain α-AAMs. Notably, β-hydroxylation demonstrated particular preferences. Specifically, ornithine, threonine, tyrosine, and phenylalanine moieties frequently underwent atypical covalent modifications, and 41 modules were used iteratively. These insights significantly facilitate the identification of uncharacterized NRPS systems-expediting traditional identification processes-although novel modifications, unusual domain organizations, and dormant domains pose challenges for their accurate prediction.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502030"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and Ultrasensitive Detection of <i>Staphylococcus aureus</i> by a One-Pot System Integrating <i>Pyrococcus furiosus</i> Argonaute with Loop-Mediated Isothermal Amplification.","authors":"Guangda Li, Jiajun Wang, Lei Tian, Mingchao Ding, Yang Liu, Jingfu Wang","doi":"10.4014/jmb.2504.04034","DOIUrl":"10.4014/jmb.2504.04034","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> (<i>S. aureus</i>) is one of the most common pathogens associated with oral and maxillofacial space infections (OMSI), significantly impairing patients' quality of life and posing substantial public health risks. Traditional detection methods are usually time-consuming and operationally complex, which limits their applicability for rapid on-site detection. This study introduces a novel, ultrasensitive nucleic acid detection system combining loop-mediated isothermal amplification (LAMP) with <i>Pyrococcus furiosus</i> Argonaute (<i>Pf</i>Ago) in a one-pot strategy. The combination of LAMP and <i>Pf</i>Ago not only improves specificity and sensitivity but also effectively reduces the risk of aerosol contamination and false positives. The system detects the <i>nuc</i> gene of <i>S. aureus</i>, enabling rapid detection within 50 min with a limit of detection (LOD) of 10⁰ Colony Forming Units (CFU)/ml for bacterial solutions and 10^-5 ng/μl for plasmids. This system exhibited exceptional specificity by effectively differentiating <i>S. aureus</i> from other common OSMI bacteria, and was successfully applied to samples from animal OMSI infection models. This innovative one-pot system offers a rapid, reliable, and effective solution for <i>S. aureus</i> detection, with potential applications in human health and public safety.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504034"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Protein Tyrosine Phosphatase 1B Inhibitors from Actinomycete Extracts Using Recombinant <i>Saccharomyces cerevisiae</i>.","authors":"Ha-Yeon Lee, Se-Young Kwun, Eun-Hee Park, Jeong-Ah Yoon, Myoung-Dong Kim","doi":"10.4014/jmb.2502.02001","DOIUrl":"10.4014/jmb.2502.02001","url":null,"abstract":"<p><p>Protein tyrosine phosphatase 1B (PTP1B) removes phosphate groups from phosphorylated tyrosine proteins in human cells, particularly in the insulin and leptin signaling pathways. It is a key drug target for ailments such as type 2 diabetes and obesity. However, there is a lack of highly specific PTP1B inhibitor drugs. This study employed recombinant <i>Saccharomyces cerevisiae</i> that co-expressed PTP1B and v-Src (viral sarcoma protein tyrosine kinase) to screen for novel PTP1B inhibitors derived from actinomycete extracts. Eight extracts significantly suppressed the growth of the recombinant <i>S. cerevisiae</i> by inhibiting PTP1B expression, indicating their potential as PTP1B inhibitors. In a protein-chip assay, actinomycete extract 4585DW showed PTP1B inhibitory activity comparable to the positive controls, suramin and vanadate. The extract was non-cytotoxic in mammalian and yeast cells and inhibited PTP1B with <i>K</i>m and <i>V</i>max values of 10.91 ± 0.50 mM and 0.02 ± 0.00 μmol/min, respectively. In conclusion, 4585DW is a promising candidate for further investigation as a PTP1B inhibitor.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502001"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal Analysis of the Immunostimulatory Properties and Safety Profile of <i>Lacticaseibacillus rhamnosus</i> LRa05 as a Dietary Supplement.","authors":"Jiayi Wu, Li Zhou, Sitong He, Xuna Tang, Jue Wang, Yanan Li","doi":"10.4014/jmb.2502.02053","DOIUrl":"10.4014/jmb.2502.02053","url":null,"abstract":"<p><p>Supplementation with appropriate doses of <i>Lacticaseibacillus rhamnosus</i> has been reported to potentially attenuate immune changes. While some progress has been made in studying the immune function of <i>L. rhamnosus</i> LRa05, a lack of systematic summaries has led to the neglect of its application in enhancing immune function. This study aimed to investigate the effects of <i>L. rhamnosus</i> LRa05 on the immune function of mice and to evaluate its safety profile as a dietary supplement. Mice were divided into three dosage groups (0.205, 0.410, and 1.230 g/kg body weight [BW]) and a negative control group (PBS), with each group orally gavaged for 30 consecutive days. The study assessed body weight, organ-to-body weight ratio, cellular immune function, humoral immune function, monocyte-macrophage phagocytic function and NK cell activity. Additionally, a subsequent 90-day subchronic oral toxicity study was conducted on rats to evaluate the safety of <i>LRa05</i> consumption. Significant differences were observed in the number of antibody-producing cells, carbon clearance function, and NK cell activity across all dosage groups compared to the negative control group, indicating that <i>L. rhamnosus</i> LRa05 has a superior ability to enhance immunity. During the 90-day subchronic oral toxicity experiment, no obvious damage was observed, demonstrating its good safety profile for consumption. This study underscores the immunopotentiating effects of <i>L. rhamnosus</i> LRa05 and its safety as a dietary supplement, recommending it as a promising candidate for integration into the food and health food industries.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502053"},"PeriodicalIF":3.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Viola tianshanica</i> Maxim Extract Ameliorates Lipopolysaccharide Induced Acute Lung Injury by Regulating NLRP3 Inflammasome and Nrf2 Signaling Pathway.","authors":"Yan Liu, Yuzhu Shi, Lei Xu, Xue Wang","doi":"10.4014/jmb.2501.01052","DOIUrl":"10.4014/jmb.2501.01052","url":null,"abstract":"<p><p>Acute lung injury (ALI) is a prevalent critical respiratory disease associated with high morbidity and mortality rates. <i>Viola tianshanica</i> Maxim has been traditionally employed in Uygur medicine for treatment of various respiratory diseases. <i>Viola tianshanica</i> Maxim extract (VTM) has strong anti-inflammatory and anti-oxidant properties, and its potential protective mechanism against ALI is worthy of further study. In this study, we investigated the protective effect of VTM on lipopolysaccharide (LPS)-induced ALI in mice and explored its underlying mechanisms involving NOD-like receptor protein 3 (NLRP3) inflammasome and Nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways. VTM was extracted from <i>Viola tianshanica</i> Maxim. Chemical compositions of VTM were identified by HPLC-HRMS/MS. The protective effect and molecular mechanisms of VTM on alleviating ALI were verified by hematoxylin-eosin (H&E) staining, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. A total of 13 chemical compositions were analyzed and identified from VTM, including esculetin, isoschaftoside, kaempferol-7-O-β-D-glucopyranoside, among others. VTM effectively alleviated ALI by reducing of the wet-to-dry weight (W/D) ratio, serum inflammatory cytokines PGE2 and LTB4 levels, and lung tissues IL-1β, IFN-γ, TNF-α and MCP-1 levels. VTM significantly decreased mRNA expressions of IL-6, TNF-α and MCP-1, inhibited MDA and MPO formation, and reversed SOD and GSH depletion. Meanwhile, VTM markedly improved histopathological changes, inhibited NLRP3 inflammasome activation by reducing the protein and mRNA expression levels of NLRP3, Caspase 1, GSDMD and IL-1β in lung tissues. Additionally, VTM mitigated oxidative stress in ALI by upregulating the protein and mRNA expression levels of Keap1, Nrf2 and HO-1 in lung tissues. Our findings indicated that pretreatment with VTM prevented LPS-induced ALI by regulating NLRP3 inflammasome and Nrf2 signaling pathway.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501052"},"PeriodicalIF":3.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12289466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Gut Microbiota Composition and Copy Number Variations in Human Genes <i>FAM66D</i> and <i>TAS2R43</i>.","authors":"Jing Wang, Haoyu Guo, Weiwei Qi, Zhenyi Qiao","doi":"10.4014/jmb.2504.04011","DOIUrl":"10.4014/jmb.2504.04011","url":null,"abstract":"<p><p>The impact of the gut microbiota on human health has attracted increasing attention. However, the factors affecting the gut microbiota need more in-depth research. In this work, we analyzed the impact of the gut microbiota at the genetic levels, mainly gene copy number variations (CNVs). We used Permutational Multivariate Analysis of Variance (PERMANOVA, Adonis) analysis to assess the association between CNVs and the gut microbiota. The results showed that copy numbers of 50 genes varied among the cohort. Among them, the CNVs of 7 genes, including <i>TBC1D3L</i>, <i>OR4C6</i>, <i>NPIPB15</i>, <i>PDPR</i>, <i>USP17L7</i>, <i>FAM66D</i> and <i>TAS2R43</i>, were related to the gut microbiota. In addition, among these genes, we systematically analyzed the relationship between CNVs of <i>FAM66D</i> and <i>TAS2R43</i> with the gut microbiota. We hypothesize that there exists a certain association between genetic information in the human genome (such as CNVs) and human behavior as well as gut microbiota.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504011"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ACC Deaminase Producing <i>Methylobacterium oryzae</i> CBMB20 and Exogenous Trehalose Application Alleviate Salinity Stress in Arabidopsis.","authors":"Kiyoon Kim, Ei Phyu Kyaw, Krishnamoorthy Ramasamy, Denver I Walitang, Raghu Rajasekaran","doi":"10.4014/jmb.2501.01007","DOIUrl":"10.4014/jmb.2501.01007","url":null,"abstract":"<p><p>Farming communities are very concerned about salt stress because of its negative impact on crop productivity. This study evaluated the ability of the <i>Methylobacterium oryzae</i> CBMB20 and exogenous trehalose treatments on <i>Arabidopsis thaliana</i> growth under salt stress conditions. <i>A. thaliana</i> growth was enhanced using <i>M. oryzae</i> CBMB20 as a bioinoculant in both saline and non-saline environments. In addition to better photosynthetic efficiency and endogenous trehalose content, the inoculation of <i>M. oryzae</i> CBMB20 produced improved growth parameters, such as increased rosette fresh weight and shoot length. Reduced levels of proline and malondialdehyde (MDA) under salt stress (150 mM NaCl) further indicated that the inoculated plants had enhanced tolerance to salinity. GFP-tagged <i>M. oryzae</i> CBMB20 was also used in spatial distribution experiments, which showed that the bacteria colonized <i>A. thaliana</i>'s root, shoot, and hypocotyl. By increasing shoot length and total fresh weight, the exogenous application of trehalose also markedly enhanced plant growth. Proline and MDA contents were decreased by exogenous trehalose during salt stress, while the endogenous trehalose concentration in <i>A. thaliana</i> remained unaffected. The use of trehalose and <i>M. oryzae</i> CBMB20 can both have a good impact on plant development and stress tolerance in saline environments.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501007"},"PeriodicalIF":2.5,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Heavy Metal Resistance in Contaminated Soil.","authors":"Tuyajargal Iimaa, Munkhjin Batmunkh, Batbold Dulguun, Batsuren Dorjsuren, Telmen Turmunkh, Enkhjargal Tserennadmid, Unursaikhan Surenjav, Battsetseg Choidash, Renchinkhand Gereltuya","doi":"10.4014/jmb.2411.11073","DOIUrl":"10.4014/jmb.2411.11073","url":null,"abstract":"<p><p>Soil heavy metal contamination poses a significant global threat to both environmental and human health. The accumulation of heavy metals in the Earth's crust, driven by urbanization, industrialization, agricultural practices, improper waste disposal, and mining, triggers harmful ecological cascades. Microorganisms, particularly bacteria, play a vital role in the decomposition and detoxification of these contaminants. In contaminated environments, bacteria exhibit resistance to heavy metals through various strategies, including the production of metal-chelating molecules, alterations to cell surface properties, efflux pumps, and activation of detoxification pathways. Notable microbial species such as <i>Bacillus</i>, <i>Enterobacter</i>, <i>Pseudomonas</i>, <i>Aspergillus</i>, and <i>Penicillium</i> show promising potential for bioremediation efforts. Harnessing bacterial resistance to heavy metals offers a cost-effective and sustainable approach to mitigate the adverse impacts of contamination. This review explores the mechanisms of heavy metal resistance in bacteria, the role of soil microbiota, and the implications for bioremediation strategies. This review emphasizes the critical importance of bacterial resistance in addressing soil contamination and highlight the need for further research to elucidate underlying mechanisms and enhance bioremediation applications in this urgent global challenge.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411073"},"PeriodicalIF":2.5,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Acute Abdomen III on Sepsis-Induced Intestinal Damage in a Rat Model.","authors":"Lu Wang, Jingjing Hu, Fangyu Hu, Yuexuan Chen, Ming Fang, Yuanling Ye","doi":"10.4014/jmb.2412.12067","DOIUrl":"10.4014/jmb.2412.12067","url":null,"abstract":"<p><p>The disruptions to intestinal integrity contribute to sepsis-related complications. Acute abdomen III is a traditional Chinese medicine formula. The objective of this research is to investigate the impact of acute abdomen III upon sepsis-induced intestinal damage. A rat model of cecal ligation and puncture was used to evaluate the impact of acute abdomen III on sepsis-induced intestinal damage. Histopathological analysis of intestinal tissue damage, detection of systemic inflammation and measurement of tight junction protein were performed by hematoxylin and eosin staining, enzyme-linked immunosorbent assay, and Western blot, respectively. Oxidative stress and intestinal barrier integrity were assessed. Terminal deoxynucleotidyl transferase dUTP nick end labeling was employed to evaluate apoptosis. Expression levels of the apoptosis-related proteins were examined. Model rats treated with acute abdomen III exhibited significantly mitigated intestinal damage. Acute abdomen III treatment reduced systemic inflammation and oxidative stress, as evidenced by downregulation of malondialdehyde and upregulation of superoxide dismutase and catalase. Acute abdomen III therapy lowered endotoxin, D-lactate, and diamine oxidase levels, while boosting the levels of tight junction proteins ZO-1, claudin-1 and occludin, implying an enhancement in intestinal barrier integrity. Acute abdomen III also markedly suppressed apoptosis in intestinal epithelial cells. Acute abdomen III can protect against sepsis-induced intestinal damage by reducing systemic inflammation and oxidative stress while promoting intestinal barrier integrity.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412067"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Loading Proximity Ligation-Induced PCR Technique for Fluorescent Detection of Intact Methicillin-Resistant <i>Staphylococcus aureus</i>.","authors":"Tingting Li, Yang Liu, Meijia Zhao, Xue Xin, Shuhong Jin","doi":"10.4014/jmb.2504.04004","DOIUrl":"10.4014/jmb.2504.04004","url":null,"abstract":"<p><p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA)-induced pneumonia in nursing patients with chronic obstructive pulmonary disease (COPD) necessitates rapid detection, timely intervention, and meticulous clinical management. Thus, the development of sensitive and accurate techniques for MRSA detection holds significant clinical nursing of infectious diseases. In this study, we designed a surface loading proximity ligation assay (PLA) for the precise detection of MRSA in pulmonary infections by simultaneously targeting three characteristic proteins. This assay utilizes three customized probes: the first probe targets protein A on the MRSA surface, the second probe immobilizes on the biological lipid layer, and the third probe identifies PBP2a (a protein responsible for drug resistance of MRSA). The proposed strategy integrates proximity ligation of these three probes with polymerase chain reaction (PCR) to perform \"AND\" logic-based analysis of the three key MRSA components, enabling sensitive detection of intact MRSA. Taking advantage of the high signal amplification efficiency of PCR and elevated target recognition capability of PLA, the method exhibited a low limit of detection of 2.9 CFU/ml. As a result, the proposed method demonstrated significantly improved accuracy for MRSA detection. We believe this novel integrated strategy could diversify existing bacterial detection approaches and may inspire the development of promising drug candidates in COPD nursing.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504004"},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}