Bin Hu, Jing Wang, Linxing Li, Qin Wang, Jingliang Qin, Yingxin Chi, Junxiang Yan, Wenkui Sun, Boyang Cao, Xi Guo
{"title":"Functional Identification and Genetic Analysis of O-Antigen Gene Clusters of Food-Borne Pathogen <i>Yersinia enterocolitica</i> O:10 and Other Uncommon Serotypes, Further Revealing Their Virulence Profiles.","authors":"Bin Hu, Jing Wang, Linxing Li, Qin Wang, Jingliang Qin, Yingxin Chi, Junxiang Yan, Wenkui Sun, Boyang Cao, Xi Guo","doi":"10.4014/jmb.2402.02044","DOIUrl":"10.4014/jmb.2402.02044","url":null,"abstract":"<p><p><i>Yersinia enterocolitica</i> is a globally distributed food-borne gastrointestinal pathogen. The O-antigen variation-determined serotype is an important characteristic of <i>Y. enterocolitica</i>, allowing intraspecies classification for diagnosis and epidemiology purposes. Among the 11 serotypes associated with human yersiniosis, O:3, O:5,27, O:8, and O:9 are the most prevalent, and their O-antigen gene clusters have been well defined. In addition to the O-antigen, several virulence factors are involved in infection and pathogenesis of <i>Y. enterocolitica</i> strains, and these are closely related to their biotypes, reflecting pathogenic properties. In this study, we identified the O-AGC of a <i>Y. enterocolitica</i> strain WL-21 of serotype O:10, and confirmed its functionality in O-antigen synthesis. Furthermore, we analyzed <i>in silico</i> the putative O-AGCs of uncommon serotypes, and found that the O-AGCs of <i>Y. enterocolitica</i> were divided into two genetic patterns: (1) O-AGC within the <i>hemH-gsk</i> locus, possibly synthesizing the O-antigen via the Wzx/Wzy dependent pathway, and (2) O-AGC within the <i>dcuC-galU-galF</i> locus, very likely assembling the O-antigen via the ABC transporter dependent pathway. By screening the virulence genes against genomes from GenBank, we discovered that strains representing different serotypes were grouped according to different virulence gene profiles, indicating strong links between serotypes and virulence markers and implying an interaction between them and the synergistic effect in pathogenicity. Our study provides a framework for further research on the origin and evolution of O-AGCs from <i>Y. enterocolitica</i>, as well as on differences in virulent mechanisms among distinct serotypes.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sequencing Methods to Study the Microbiome with Antibiotic Resistance Genes in Patients with Pulmonary Infections.","authors":"Tingyan Dong, Yongsi Wang, Chunxia Qi, Wentao Fan, Junting Xie, Haitao Chen, Hao Zhou, Xiaodong Han","doi":"10.4014/jmb.2402.02004","DOIUrl":"10.4014/jmb.2402.02004","url":null,"abstract":"<p><p>Various antibiotic-resistant bacteria (ARB) are known to induce repeated pulmonary infections and increase morbidity and mortality. A thorough knowledge of antibiotic resistance is imperative for clinical practice to treat resistant pulmonary infections. In this study, we used a reads-based method and an assembly-based method according to the metagenomic next-generation sequencing (mNGS) data to reveal the spectra of ARB and corresponding antibiotic resistance genes (ARGs) in samples from patients with pulmonary infections. A total of 151 clinical samples from 144 patients with pulmonary infections were collected for retrospective analysis. The ARB and ARGs detection performance was compared by the reads-based method and assembly-based method with the culture method and antibiotic susceptibility testing (AST), respectively. In addition, ARGs and the attribution relationship of common ARB were analyzed by the two methods. The comparison results showed that the assembly-based method could assist in determining pathogens detected by the reads-based method as true ARB and improve the predictive capabilities (46% > 13%). ARG-ARB network analysis revealed that assembly-based method could promote determining clear ARG-bacteria attribution and 101 ARGs were detected both in two methods. 25 ARB were obtained by both methods, of which the most predominant ARB and its ARGs in the samples of pulmonary infections were <i>Acinetobacter baumannii</i> (<i>ade</i>), <i>Pseudomonas aeruginosa</i> (<i>mex</i>), <i>Klebsiella pneumoniae</i> (<i>emr</i>), and <i>Stenotrophomonas maltophilia</i> (<i>sme</i>). Collectively, our findings demonstrated that the assembly-based method could be a supplement to the reads-based method and uncovered pulmonary infection-associated ARB and ARGs as potential antibiotic treatment targets.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yena Nam, Eunju Cha, Su Min Kwak, Seung Ju Seo, John Hoon Rim, Yoonhee Jin
{"title":"Harnessing Decellularized Extracellular Matrix for Enhanced Fidelity in Colorectal Cancer Organoid and Cell-Derived Xenograft Models.","authors":"Yena Nam, Eunju Cha, Su Min Kwak, Seung Ju Seo, John Hoon Rim, Yoonhee Jin","doi":"10.4014/jmb.2405.05036","DOIUrl":"10.4014/jmb.2405.05036","url":null,"abstract":"<p><p>This study evaluates the efficacy of a decellularized intestine tissue-derived extracellular matrix (Intestine ECM) as a scaffold for culturing colorectal cancer (CRC) organoids and establishing cell-derived xenograft (CDX) models, comparing its performance to traditional Matrigel. Intestine ECM demonstrates comparable support for organoid formation and cellular function, highlighting its potential as a more physiologically relevant and reproducible platform. Our findings suggest that Intestine ECM enhances the mimetic environment for colon epithelium, supporting comparable growth and improved differentiation compared to Matrigel. Moreover, when used as a delivery carrier, Intestine ECM significantly increases the growth rate of CDX models using patient-derived primary colorectal cancer cells. This enhancement demonstrates Intestine ECM's role not only as a scaffold but also as a vital component of the tumor microenvironment, facilitating more robust tumorigenesis. These findings advocate for the broader application of Intestine ECM in cancer model systems, potentially leading to more accurate preclinical evaluations and the development of targeted cancer therapies.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Woo-Jin Kim, Ah-Ra Lee, Su-Yeon Hong, Sang-Hyun Kim, Jae-Deog Kim, Sung Jae Kim, Jae Sang Oh, Sang-Mu Shim, Sang-Uk Seo
{"title":"Characterization of a Small Plaque Variant Derived from Genotype V Japanese Encephalitis Virus Clinical Isolate K15P38.","authors":"Woo-Jin Kim, Ah-Ra Lee, Su-Yeon Hong, Sang-Hyun Kim, Jae-Deog Kim, Sung Jae Kim, Jae Sang Oh, Sang-Mu Shim, Sang-Uk Seo","doi":"10.4014/jmb.2404.04054","DOIUrl":"10.4014/jmb.2404.04054","url":null,"abstract":"<p><p>Genotype V (GV) Japanese encephalitis virus (JEV) has been predominantly reported in the Republic of Korea (ROK) since 2010. GV JEV exhibits higher virulence and distinct antigenicity compared to other genotypes, which results in reduced efficacy of existing vaccines. Research on GV JEV is essential to minimize its clinical impact, but the only available clinical strain in the ROK is K15P38, isolated from the cerebrospinal fluid of a patient in 2015. We obtained this virus from National Culture Collection for Pathogens (NCCP) and isolated a variant forming small plaques during our research. We identified that this variant has one amino acid substitution each in the PrM and NS5 proteins compared to the reported K15P38. Additionally, we confirmed that this virus exhibits delayed propagation in vitro and an attenuated phenotype in mice. The isolation of this variant is a critical reference for researchers intending to study K15P38 obtained from NCCP, and the mutations in the small plaque-forming virus are expected to be useful for studying the pathology of GV JEV.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Siderophore Biosynthesis and Transport Systems in Model and Pathogenic Fungi.","authors":"Sohyeong Choi, James W Kronstad, Won Hee Jung","doi":"10.4014/jmb.2405.05020","DOIUrl":"10.4014/jmb.2405.05020","url":null,"abstract":"<p><p>Fungi employ diverse mechanisms for iron uptake to ensure proliferation and survival in iron-limited environments. Siderophores are secondary metabolite small molecules with a high affinity specifically for ferric iron; these molecules play an essential role in iron acquisition in fungi and significantly influence fungal physiology and virulence. Fungal siderophores, which are primarily hydroxamate types, are synthesized via non-ribosomal peptide synthetases (NRPS) or NRPS-independent pathways. Following synthesis, siderophores are excreted, chelate iron, and are transported into the cell by specific cell membrane transporters. In several human pathogenic fungi, siderophores are pivotal for virulence, as inhibition of their synthesis or transport significantly reduces disease in murine models of infection. This review briefly highlights siderophore biosynthesis and transport mechanisms in fungal pathogens as well the model fungi <i>Saccharomyces cerevisiae</i> and <i>Schizosaccharomyces pombe</i>. Understanding siderophore biosynthesis and transport in pathogenic fungi provides valuable insights into fungal biology and illuminates potential therapeutic targets for combating fungal infections.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advances in the Structures, Pharmacological Activities, and Biosynthesis of Plant Diterpenoids.","authors":"Leilei Li, Jia Fu, Nan Liu","doi":"10.4014/jmb.2402.02014","DOIUrl":"10.4014/jmb.2402.02014","url":null,"abstract":"<p><p>More and more diterpenoids have attracted extensive attention due to the diverse chemical structures and excellent biological activities, and have been developed into clinical drugs or consumer products. The vast majority of diterpenoids are derived from plants. With the long-term development of plant medicinal materials, the natural resources of many plant diterpenoids are decreasing, and the biosynthetic mechanism of key active components has increasingly become a research hotspot. Using synthetic biology to engineer microorganisms into \"cell factories\" to produce the desired compounds is an essential means to solve these problems. In this review, we depict the plant-derived diterpenoids from chemical structure, biological activities, and biosynthetic pathways. We use representative plant diterpenes as examples to expound the research progress on their biosynthesis, and summarize the heterologous production of plant diterpenoids in microorganisms in recent years, hoping to lay the foundation for the development and application of plant diterpenoids in the future.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thi-Phuong Nguyen, Tang Van Duong, Thai Quang Le, Khoa Thi Nguyen
{"title":"Uncovering the Antibacterial Potential of a Peptide-Rich Extract of Edible Bird's Nest against <i>Staphylococcus aureus</i>.","authors":"Thi-Phuong Nguyen, Tang Van Duong, Thai Quang Le, Khoa Thi Nguyen","doi":"10.4014/jmb.2402.02052","DOIUrl":"10.4014/jmb.2402.02052","url":null,"abstract":"<p><p>The diverse pharmacological properties of edible bird's nest (EBN) have been elucidated in recent years; however, investigations into its antibacterial effects are still limited. In the present study, we explored the antibacterial activity of a peptide-rich extract of EBN against <i>Staphylococcus aureus</i>, a notorious pathogen. The EBN extract (EEE) was prepared by soaking EBN in 80% ethanol for 2 days at 60°C. Biochemical analyses showed that peptides at the molecular weight range of 1.7-10 kDa were the major biochemical compounds in the EEE. The extract exhibited strong inhibition against <i>S. aureus</i> at a minimum inhibitory concentration (MIC) of 125 μg/ml and a minimum bactericidal concentration (MBC) of 250 μg/ml. This activity could be attributed to the impact of the extract on cell membrane integrity and potential, biofilm formation, and reactive oxidative species (ROS) production. Notably, the expression of biofilm- and ROS-associated genes, including <i>intercellular adhesion A</i> (<i>icaA</i>), <i>icaB</i>, <i>icaC</i>, <i>icaD</i>, and <i>superoxide dismutase A</i> (<i>sodA</i>), were deregulated in <i>S. aureus</i> upon the extract treatment. Our findings indicate a noteworthy pharmacological activity of EBN that could have potential application in the control of <i>S. aureus</i>.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xueqin Xu, Qianqian Wang, Longyan Yang, Zhiyan Chen, Yun Zhou, Hui Feng, Peng Zhang, Jie Wang
{"title":"Effects of Exocellobiohydrolase CBHA on Fermentation of Tobacco Leaves.","authors":"Xueqin Xu, Qianqian Wang, Longyan Yang, Zhiyan Chen, Yun Zhou, Hui Feng, Peng Zhang, Jie Wang","doi":"10.4014/jmb.2404.04028","DOIUrl":"10.4014/jmb.2404.04028","url":null,"abstract":"<p><p>The quality of tobacco is directly affected by macromolecular content, fermentation is an effective method to improve biochemical properties. In this study, we utilized CBHA (cellobiohydrolase A) glycosylase, which was expressed by <i>Pichia pastoris</i>, as an additive for fermentation. The contents of main chemical components of tobacco leaves after fermentation were determined, and the changes of microbial community structure and abundance in tobacco leaves during fermentation were analyzed. The relationship between chemical composition and changes in microbial composition was investigated, and the function of bacteria and fungi in fermentation was predicted to identify possible metabolic pathways. After 48 h of CBHA fermentation, the contents of starch, cellulose and total nitrogen in tobacco leaf decreased by 17.60%, 28.91% and 16.05%, respectively. The microbial community structure changed significantly, with <i>Aspergillus</i> abundance decreasing significantly, while <i>Filobasidum</i>, <i>Cladosporium</i>, <i>Bullera</i>, <i>Komagataella</i>, etc., increased in CBHA treated group. Soluble sugar was most affected by microbial community in tobacco leaves, which was negatively correlated with starch, cellulose and total nitrogen. During the fermentation process, the relative abundance of metabolism-related functional genes increased, and the expressions of cellulase and endopeptidase also increased. The results showed that the changes of bacterial community and dominant microbial community on tobacco leaves affected the content of chemical components in tobacco leaves, and adding CBHA for fermentation had a positive effect on improving the quality of tobacco leaves.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yoon-Jung Choi, Shukho Kim, Ram Hari Dahal, Jungmin Kim
{"title":"A Novel Truncated CHAP Modular Endolysin, CHAP<sup>SAP26</sup>-161, That Lyses <i>Staphylococcus aureus</i>, <i>Acinetobacter baumannii</i>, and <i>Clostridioides difficile</i>, and Exhibits Therapeutic Effects in a Mouse Model of <i>A. baumannii</i> Infection.","authors":"Yoon-Jung Choi, Shukho Kim, Ram Hari Dahal, Jungmin Kim","doi":"10.4014/jmb.2402.02042","DOIUrl":"10.4014/jmb.2402.02042","url":null,"abstract":"<p><p>Development of novel antibacterial agents is imperative due to the increasing threat of antibiotic-resistant pathogens. This study aimed to develop the enhanced antibacterial activity and in-vivo efficacy of a novel truncated endolysin, CHAP<sup>SAP26</sup>-161, derived from the endolysin LysSAP26, against multidrug-resistant bacteria. CHAP<sup>SAP26</sup>-161 exhibited higher protein purification efficiency in E. coli and antibacterial activity than LysSAP26. Moreover, CHAP<sup>SAP26</sup>-161 showed the higher lytic activity against <i>A. baumannii</i> with minimal bactericidal concentrations (MBCs) of 5-10 μg/ml, followed by <i>Staphylococcus aureus</i> with MBCs of 10-25 μg/ml. Interestingly, CHAP<sup>SAP26</sup>-161 could lyse anaerobic bacteria, such as <i>Clostridioides difficile</i>, with MBCs of 25-50 μg/ml. At pH 4-8 and temperatures of 4°C-45°C, CHAP<sup>SAP26</sup>-161 maintained antibacterial activity without remarkable difference. The lytic activity of CHAP<sup>SAP26</sup>-161 was increased with Zn<sup>2+</sup>. In vivo tests demonstrated the therapeutic effects of CHAP<sup>SAP26</sup>-161 in murine systemic <i>A. baumannii</i> infection model. In conclusion, CHAP<sup>SAP26</sup>-161, a truncated endolysin that retains only the CHAP domain from LysSAP26, demonstrated enhanced protein purification efficiency and antibacterial activity compared to LysSAP26. It further displayed broad-spectrum antibacterial effects against <i>S. aureus</i>, <i>A. baumannii</i>, and <i>C. difficile</i>. Our in vitro and in-vivo results of CHAP<sup>SAP26</sup>-161 highlights its promise as an innovative therapeutic option against those bacteria with multiple antibiotic resistance.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring Levansucrase Operon Regulating Levan-Type Fructooligosaccharides Production in Priestia koreensis HL12.","authors":"Hataikarn Lekakarn,Daran Prongjit,Wuttichai Mhuantong,Srisakul Trakarnpaiboon,Benjarat Bunterngsook","doi":"10.4014/jmb.2404.04043","DOIUrl":"https://doi.org/10.4014/jmb.2404.04043","url":null,"abstract":"Levan biopolymer and levan-type fructooligosaccharides (L-FOSs) are β-2,6-linked fructans that have been used as non-digestible dietary fiber and prebiotic oligosaccharides in food and cosmeceutical applications. In this study, we explore the operon responsible for levan and lL-FOSs production in Priestia koreensis HL12. Presented is the first genomic perspective on sucrose utilization and the levan biosynthesis pathway in this bacterium. Regarding sequence annotation, the putative levansucrase operon responsible for β-2,6-linked fructan was identified in the genome of strain HL12, and comprises sacB levansucrase gene belonging to GH68, located adjacent to levB endo-levanase gene, which belongs to GH32. Importantly, sugars related with the levan biosynthesis pathway are proposed to be transported via 3 types of transportation systems, including multiple ABCSugar and glucose/H+ transporters, as well as glucoseand fructose-specific PTS systems. Based on product profile analysis, the HL12 strain exhibited high efficiency in levan production from high sucrose concentration (300 g/l), achieving the highest yield of 127 g/l (equivalent to 55% conversion based on sucrose consumption), together with short-chain L-FOSs (DP3-5) and long-chain L-FOSs with respective size larger than DP6 after 48 h incubation. These findings highlight the potential of P. koreensis HL12 as a whole-cell biocatalyst for producing levan and L-FOSs, and underscore its novelty in converting sugars into high-value-added products for diverse commercial and industrial applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}