Journal of microbiology and biotechnology最新文献_第9页

Arctigenin from Burdock Root Exhibits Potent Antibacterial and Anti-Virulence Properties against Pseudomonas aeruginosa. 牛蒡根中的牛蒡苷元对铜绿假单胞菌具有强效抗菌和抗病毒作用

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-17 DOI: 10.4014/jmb.2403.03003

Abdulrahman E Koshak, Mahmoud A Elfaky, Hossam M Abdallah, Dina A I Albadawi, Gamal A Mohamed, Sabrin R M Ibrahim, Abdulrahim A Alzain, El-Sayed Khafagy, Azza A H Rajab, Wael A H Hegazy

{"title":"Arctigenin from Burdock Root Exhibits Potent Antibacterial and Anti-Virulence Properties against Pseudomonas aeruginosa.","authors":"Abdulrahman E Koshak, Mahmoud A Elfaky, Hossam M Abdallah, Dina A I Albadawi, Gamal A Mohamed, Sabrin R M Ibrahim, Abdulrahim A Alzain, El-Sayed Khafagy, Azza A H Rajab, Wael A H Hegazy","doi":"10.4014/jmb.2403.03003","DOIUrl":"10.4014/jmb.2403.03003","url":null,"abstract":"Arctium lappa (Burdock) root is used in various culinary applications especially in Asian Cuisine. Arctigenin (ARC) is a polyphenolic compound abundant in the roots of the burdock plant from which it derives its name. The emergence of bacterial resistance is a growing global worry, specifically due to the declining availability of new antibiotics. Screening for the antibacterial candidates among the safe natural products is a promising approach. The present study was aimed to assess the antibacterial activity of ARC against Pseudomonas aeruginosa exploring its effect on the bacterial cell membrane. Furthermore, the anti-virulence activities and anti-quorum sensing (QS) activities of ARC were in vitro, in vivo and in silico assessed against P. aeruginosa. The current results showed the ARC antibacterial activity was owed to its disruption effect of the cell membrane. ARC at sub-MIC significantly decreased the formation of biofilm, motility, production of extracellular enzymes and in vivo protected mice against P. aeruginosa. These anti-virulence activities of ARC are owed to its interference with bacterial QS and its expression. Furthermore, ARC showed mild effect on mammalian erythrocytes, low probability to induce resistance and synergistically combined with antibiotics. In summary, the promising anti-virulence properties of ARC indicate its potential as an effective supplement to conventional antibiotics for treating severe P. aeruginosa infections.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1642-1652"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kimchi Lactic Acid Bacteria Starter Culture: Impact on Fermented Malt Beverage Volatile Profile, Sensory Analysis, and Physicochemical Traits. 泡菜乳酸菌起始培养物：对发酵麦芽饮料挥发性成分、感官分析和理化特性的影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-28 DOI: 10.4014/jmb.2403.03011

Moeun Lee, Daun Kim, Ki Won Lee, Ji Yoon Chang

{"title":"Kimchi Lactic Acid Bacteria Starter Culture: Impact on Fermented Malt Beverage Volatile Profile, Sensory Analysis, and Physicochemical Traits.","authors":"Moeun Lee, Daun Kim, Ki Won Lee, Ji Yoon Chang","doi":"10.4014/jmb.2403.03011","DOIUrl":"10.4014/jmb.2403.03011","url":null,"abstract":"Starter cultures used during the fermentation of malt wort can increase the sensory characteristics of the resulting beverages. This study aimed to explore the aroma composition and flavor recognition of malt wort beverages fermented with lactic acid bacteria (Levilactobacillus brevis WiKim0194) isolated from kimchi, using metabolomic profiling and electronic tongue and nose technologies. Four sugars and five organic acids were detected using high-performance liquid chromatography, with maltose and lactic acid present in the highest amounts. Additionally, e-tongue measurements showed a significant increase in the sourness (AHS), sweetness (ANS), and umami (NMS) sensors, whereas bitterness (SCS) significantly decreased. Furthermore, 20 key aroma compounds were identified using gas chromatography-mass spectrometry and 15 key aroma flavors were detected using an electronic nose. Vanillin, citronellol, and β-damascenone exhibited significant differences in the flavor profile of the beverage fermented by WiKim0194, which correlated with floral, fruity, and sweet notes. Therefore, we suggest that an appropriate starter culture can improve sensory characteristics and predict flavor development in malt wort beverages.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1653-1659"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutations Affecting Cellular Levels of Cobalamin (Vitamin B₁₂) Confer Tolerance to Bactericidal Antibiotics in Burkholderia cenocepacia. 影响钴胺素（维生素 B12）细胞水平的突变使伯克霍尔德氏菌（Burkholderia cenocepacia）耐受杀菌抗生素。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-07-11 DOI: 10.4014/jmb.2406.06028

Dongju Lee, Jongwook Park, Heenam Stanley Kim

{"title":"Mutations Affecting Cellular Levels of Cobalamin (Vitamin B12) Confer Tolerance to Bactericidal Antibiotics in Burkholderia cenocepacia.","authors":"Dongju Lee, Jongwook Park, Heenam Stanley Kim","doi":"10.4014/jmb.2406.06028","DOIUrl":"10.4014/jmb.2406.06028","url":null,"abstract":"The Burkholderia cepacia complex (Bcc) consists of opportunistic pathogens known to cause pneumonia in immunocompromised individuals, especially those with cystic fibrosis. Treating Bcc pneumonia is challenging due to the pathogens' high multidrug resistance. Therefore, inhalation therapy with tobramycin powder, which can achieve high antibiotic concentrations in the lungs, is a promising treatment option. In this study, we investigated potential mechanisms that could compromise the effectiveness of tobramycin therapy. By selecting for B. cenocepacia survivors against tobramycin, we identified three spontaneous mutations that disrupt a gene encoding a key enzyme in the biosynthesis of cobalamin (Vitamin B12). This disruption may affect the production of succinyl-CoA by methylmalonyl-CoA mutase, which requires adenosylcobalamin as a cofactor. The depletion of cellular succinyl-CoA may impact the tricarboxylic acid (TCA) cycle, which becomes metabolically overloaded upon exposure to tobramycin. Consequently, the mutants exhibited significantly reduced reactive oxygen species (ROS) production. Both the wild-type and mutants showed tolerance to tobramycin and various other bactericidal antibiotics under microaerobic conditions. This suggests that compromised ROS-mediated killing, due to the impacted TCA cycle, underlies the mutants' tolerance to bactericidal antibiotics. The importance of ROS-mediated killing and the potential emergence of mutants that evade it through the depletion of cobalamin (Vitamin B12) provide valuable insights for developing strategies to enhance antibiotic treatments of Bcc pneumonia.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1609-1616"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Identification and Genetic Analysis of O-Antigen Gene Clusters of Food-Borne Pathogen Yersinia enterocolitica O:10 and Other Uncommon Serotypes, Further Revealing Their Virulence Profiles. 食源性病原体小肠结肠耶尔森菌 O:10 和其他不常见血清型 O 抗原基因簇的功能鉴定和遗传分析，进一步揭示其毒性特征

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-07-15 DOI: 10.4014/jmb.2402.02044

Bin Hu, Jing Wang, Linxing Li, Qin Wang, Jingliang Qin, Yingxin Chi, Junxiang Yan, Wenkui Sun, Boyang Cao, Xi Guo

{"title":"Functional Identification and Genetic Analysis of O-Antigen Gene Clusters of Food-Borne Pathogen Yersinia enterocolitica O:10 and Other Uncommon Serotypes, Further Revealing Their Virulence Profiles.","authors":"Bin Hu, Jing Wang, Linxing Li, Qin Wang, Jingliang Qin, Yingxin Chi, Junxiang Yan, Wenkui Sun, Boyang Cao, Xi Guo","doi":"10.4014/jmb.2402.02044","DOIUrl":"10.4014/jmb.2402.02044","url":null,"abstract":"Yersinia enterocolitica is a globally distributed food-borne gastrointestinal pathogen. The O-antigen variation-determined serotype is an important characteristic of Y. enterocolitica, allowing intraspecies classification for diagnosis and epidemiology purposes. Among the 11 serotypes associated with human yersiniosis, O:3, O:5,27, O:8, and O:9 are the most prevalent, and their O-antigen gene clusters have been well defined. In addition to the O-antigen, several virulence factors are involved in infection and pathogenesis of Y. enterocolitica strains, and these are closely related to their biotypes, reflecting pathogenic properties. In this study, we identified the O-AGC of a Y. enterocolitica strain WL-21 of serotype O:10, and confirmed its functionality in O-antigen synthesis. Furthermore, we analyzed in silico the putative O-AGCs of uncommon serotypes, and found that the O-AGCs of Y. enterocolitica were divided into two genetic patterns: (1) O-AGC within the hemH-gsk locus, possibly synthesizing the O-antigen via the Wzx/Wzy dependent pathway, and (2) O-AGC within the dcuC-galU-galF locus, very likely assembling the O-antigen via the ABC transporter dependent pathway. By screening the virulence genes against genomes from GenBank, we discovered that strains representing different serotypes were grouped according to different virulence gene profiles, indicating strong links between serotypes and virulence markers and implying an interaction between them and the synergistic effect in pathogenicity. Our study provides a framework for further research on the origin and evolution of O-AGCs from Y. enterocolitica, as well as on differences in virulent mechanisms among distinct serotypes.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1599-1608"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequencing Methods to Study the Microbiome with Antibiotic Resistance Genes in Patients with Pulmonary Infections. 用测序方法研究肺部感染患者带有抗生素耐药基因的微生物组。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-20 DOI: 10.4014/jmb.2402.02004

Tingyan Dong, Yongsi Wang, Chunxia Qi, Wentao Fan, Junting Xie, Haitao Chen, Hao Zhou, Xiaodong Han

{"title":"Sequencing Methods to Study the Microbiome with Antibiotic Resistance Genes in Patients with Pulmonary Infections.","authors":"Tingyan Dong, Yongsi Wang, Chunxia Qi, Wentao Fan, Junting Xie, Haitao Chen, Hao Zhou, Xiaodong Han","doi":"10.4014/jmb.2402.02004","DOIUrl":"10.4014/jmb.2402.02004","url":null,"abstract":"Various antibiotic-resistant bacteria (ARB) are known to induce repeated pulmonary infections and increase morbidity and mortality. A thorough knowledge of antibiotic resistance is imperative for clinical practice to treat resistant pulmonary infections. In this study, we used a reads-based method and an assembly-based method according to the metagenomic next-generation sequencing (mNGS) data to reveal the spectra of ARB and corresponding antibiotic resistance genes (ARGs) in samples from patients with pulmonary infections. A total of 151 clinical samples from 144 patients with pulmonary infections were collected for retrospective analysis. The ARB and ARGs detection performance was compared by the reads-based method and assembly-based method with the culture method and antibiotic susceptibility testing (AST), respectively. In addition, ARGs and the attribution relationship of common ARB were analyzed by the two methods. The comparison results showed that the assembly-based method could assist in determining pathogens detected by the reads-based method as true ARB and improve the predictive capabilities (46% > 13%). ARG-ARB network analysis revealed that assembly-based method could promote determining clear ARG-bacteria attribution and 101 ARGs were detected both in two methods. 25 ARB were obtained by both methods, of which the most predominant ARB and its ARGs in the samples of pulmonary infections were Acinetobacter baumannii (ade), Pseudomonas aeruginosa (mex), Klebsiella pneumoniae (emr), and Stenotrophomonas maltophilia (sme). Collectively, our findings demonstrated that the assembly-based method could be a supplement to the reads-based method and uncovered pulmonary infection-associated ARB and ARGs as potential antibiotic treatment targets.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1617-1626"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Siderophore Biosynthesis and Transport Systems in Model and Pathogenic Fungi. 模式真菌和致病真菌中的苷酸生物合成和运输系统

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-13 DOI: 10.4014/jmb.2405.05020

Sohyeong Choi, James W Kronstad, Won Hee Jung

{"title":"Siderophore Biosynthesis and Transport Systems in Model and Pathogenic Fungi.","authors":"Sohyeong Choi, James W Kronstad, Won Hee Jung","doi":"10.4014/jmb.2405.05020","DOIUrl":"10.4014/jmb.2405.05020","url":null,"abstract":"Fungi employ diverse mechanisms for iron uptake to ensure proliferation and survival in iron-limited environments. Siderophores are secondary metabolite small molecules with a high affinity specifically for ferric iron; these molecules play an essential role in iron acquisition in fungi and significantly influence fungal physiology and virulence. Fungal siderophores, which are primarily hydroxamate types, are synthesized via non-ribosomal peptide synthetases (NRPS) or NRPS-independent pathways. Following synthesis, siderophores are excreted, chelate iron, and are transported into the cell by specific cell membrane transporters. In several human pathogenic fungi, siderophores are pivotal for virulence, as inhibition of their synthesis or transport significantly reduces disease in murine models of infection. This review briefly highlights siderophore biosynthesis and transport mechanisms in fungal pathogens as well the model fungi Saccharomyces cerevisiae and Schizosaccharomyces pombe. Understanding siderophore biosynthesis and transport in pathogenic fungi provides valuable insights into fungal biology and illuminates potential therapeutic targets for combating fungal infections.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1551-1562"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Decellularized Extracellular Matrix for Enhanced Fidelity in Colorectal Cancer Organoid and Cell-Derived Xenograft Models. 利用脱细胞细胞外基质提高结直肠癌类器官和细胞衍生异种移植模型的保真度

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-27 DOI: 10.4014/jmb.2405.05036

Yena Nam, Eunju Cha, Su Min Kwak, Seung Ju Seo, John Hoon Rim, Yoonhee Jin

{"title":"Harnessing Decellularized Extracellular Matrix for Enhanced Fidelity in Colorectal Cancer Organoid and Cell-Derived Xenograft Models.","authors":"Yena Nam, Eunju Cha, Su Min Kwak, Seung Ju Seo, John Hoon Rim, Yoonhee Jin","doi":"10.4014/jmb.2405.05036","DOIUrl":"10.4014/jmb.2405.05036","url":null,"abstract":"This study evaluates the efficacy of a decellularized intestine tissue-derived extracellular matrix (Intestine ECM) as a scaffold for culturing colorectal cancer (CRC) organoids and establishing cell-derived xenograft (CDX) models, comparing its performance to traditional Matrigel. Intestine ECM demonstrates comparable support for organoid formation and cellular function, highlighting its potential as a more physiologically relevant and reproducible platform. Our findings suggest that Intestine ECM enhances the mimetic environment for colon epithelium, supporting comparable growth and improved differentiation compared to Matrigel. Moreover, when used as a delivery carrier, Intestine ECM significantly increases the growth rate of CDX models using patient-derived primary colorectal cancer cells. This enhancement demonstrates Intestine ECM's role not only as a scaffold but also as a vital component of the tumor microenvironment, facilitating more robust tumorigenesis. These findings advocate for the broader application of Intestine ECM in cancer model systems, potentially leading to more accurate preclinical evaluations and the development of targeted cancer therapies.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1711-1717"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a Small Plaque Variant Derived from Genotype V Japanese Encephalitis Virus Clinical Isolate K15P38. 源于基因型 V 日本脑炎病毒临床样本 K15P38 的小斑块变异体的特征。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-23 DOI: 10.4014/jmb.2404.04054

Woo-Jin Kim, Ah-Ra Lee, Su-Yeon Hong, Sang-Hyun Kim, Jae-Deog Kim, Sung Jae Kim, Jae Sang Oh, Sang-Mu Shim, Sang-Uk Seo

{"title":"Characterization of a Small Plaque Variant Derived from Genotype V Japanese Encephalitis Virus Clinical Isolate K15P38.","authors":"Woo-Jin Kim, Ah-Ra Lee, Su-Yeon Hong, Sang-Hyun Kim, Jae-Deog Kim, Sung Jae Kim, Jae Sang Oh, Sang-Mu Shim, Sang-Uk Seo","doi":"10.4014/jmb.2404.04054","DOIUrl":"10.4014/jmb.2404.04054","url":null,"abstract":"Genotype V (GV) Japanese encephalitis virus (JEV) has been predominantly reported in the Republic of Korea (ROK) since 2010. GV JEV exhibits higher virulence and distinct antigenicity compared to other genotypes, which results in reduced efficacy of existing vaccines. Research on GV JEV is essential to minimize its clinical impact, but the only available clinical strain in the ROK is K15P38, isolated from the cerebrospinal fluid of a patient in 2015. We obtained this virus from National Culture Collection for Pathogens (NCCP) and isolated a variant forming small plaques during our research. We identified that this variant has one amino acid substitution each in the PrM and NS5 proteins compared to the reported K15P38. Additionally, we confirmed that this virus exhibits delayed propagation in vitro and an attenuated phenotype in mice. The isolation of this variant is a critical reference for researchers intending to study K15P38 obtained from NCCP, and the mutations in the small plaque-forming virus are expected to be useful for studying the pathology of GV JEV.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1592-1598"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the Structures, Pharmacological Activities, and Biosynthesis of Plant Diterpenoids. 植物二萜的结构、药理活性和生物合成研究进展》。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-06-17 DOI: 10.4014/jmb.2402.02014

Leilei Li, Jia Fu, Nan Liu

{"title":"Advances in the Structures, Pharmacological Activities, and Biosynthesis of Plant Diterpenoids.","authors":"Leilei Li, Jia Fu, Nan Liu","doi":"10.4014/jmb.2402.02014","DOIUrl":"10.4014/jmb.2402.02014","url":null,"abstract":"More and more diterpenoids have attracted extensive attention due to the diverse chemical structures and excellent biological activities, and have been developed into clinical drugs or consumer products. The vast majority of diterpenoids are derived from plants. With the long-term development of plant medicinal materials, the natural resources of many plant diterpenoids are decreasing, and the biosynthetic mechanism of key active components has increasingly become a research hotspot. Using synthetic biology to engineer microorganisms into \"cell factories\" to produce the desired compounds is an essential means to solve these problems. In this review, we depict the plant-derived diterpenoids from chemical structure, biological activities, and biosynthetic pathways. We use representative plant diterpenes as examples to expound the research progress on their biosynthesis, and summarize the heterologous production of plant diterpenoids in microorganisms in recent years, hoping to lay the foundation for the development and application of plant diterpenoids in the future.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1563-1579"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the Antibacterial Potential of a Peptide-Rich Extract of Edible Bird's Nest against Staphylococcus aureus. 揭示食用燕窝富含肽提取物对金黄色葡萄球菌的抗菌潜力

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-08-28 Epub Date: 2024-07-12 DOI: 10.4014/jmb.2402.02052

Thi-Phuong Nguyen, Tang Van Duong, Thai Quang Le, Khoa Thi Nguyen

{"title":"Uncovering the Antibacterial Potential of a Peptide-Rich Extract of Edible Bird's Nest against Staphylococcus aureus.","authors":"Thi-Phuong Nguyen, Tang Van Duong, Thai Quang Le, Khoa Thi Nguyen","doi":"10.4014/jmb.2402.02052","DOIUrl":"10.4014/jmb.2402.02052","url":null,"abstract":"The diverse pharmacological properties of edible bird's nest (EBN) have been elucidated in recent years; however, investigations into its antibacterial effects are still limited. In the present study, we explored the antibacterial activity of a peptide-rich extract of EBN against Staphylococcus aureus, a notorious pathogen. The EBN extract (EEE) was prepared by soaking EBN in 80% ethanol for 2 days at 60°C. Biochemical analyses showed that peptides at the molecular weight range of 1.7-10 kDa were the major biochemical compounds in the EEE. The extract exhibited strong inhibition against S. aureus at a minimum inhibitory concentration (MIC) of 125 μg/ml and a minimum bactericidal concentration (MBC) of 250 μg/ml. This activity could be attributed to the impact of the extract on cell membrane integrity and potential, biofilm formation, and reactive oxidative species (ROS) production. Notably, the expression of biofilm- and ROS-associated genes, including intercellular adhesion A (icaA), icaB, icaC, icaD, and superoxide dismutase A (sodA), were deregulated in S. aureus upon the extract treatment. Our findings indicate a noteworthy pharmacological activity of EBN that could have potential application in the control of S. aureus.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 8","pages":"1680-1687"},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0