Journal of microbiology and biotechnology最新文献

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Molecular Characterization and Interaction between Human VEGF-D and VEGFR-3. 人血管内皮生长因子-D 和血管内皮生长因子受体-3 之间的分子特征和相互作用。
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-20 DOI: 10.4014/jmb.2409.09060
Chae Eun Seo, Han Na Lee, Mi Suk Jeong, Se Bok Jang
{"title":"Molecular Characterization and Interaction between Human VEGF-D and VEGFR-3.","authors":"Chae Eun Seo, Han Na Lee, Mi Suk Jeong, Se Bok Jang","doi":"10.4014/jmb.2409.09060","DOIUrl":"10.4014/jmb.2409.09060","url":null,"abstract":"<p><p>Angiogenesis and lymphangiogenesis are some of the routes that cause metastasis. Vascular Endothelial Growth Factors (VEGFs) stimulate angiogenesis and lymphangiogenesis through VEGF receptors. Especially, VEGF-D and its receptor, VEGFR-3, play a pivotal role in regulating cellular processes such as survival, proliferation, and migration, thereby influencing lymphangiogenesis. The aim of this research is to clarify the molecular characteristics of VEGF-D and VEGFR-3 proteins and identify the key residues that are essential for the interaction between VEGF-D and VEGFR-3. Experiments, including size exclusion chromatography and GST pull-down assay analysis, reveal that specific residues, particularly D103 and Q110, are essential for VEGF-D/VEGFR-3 binding. Mutations in these residues induce structural alterations, resulting in reduced binding affinity and impaired activation of VEGFR-3. Moreover, this study suggests that a synthesized peptide, designed based on key residues of VEGF-D involved in binding to VEGFR-3, may act as a metastasis suppressor by competitively inhibiting the interaction between VEGF-D and VEGFR-3. Understanding these molecular interactions is expected to have significant potential to develop therapeutic peptides that can inhibit cancer cell-induced lymphangiogenesis and resolve metastasis via lymphangiogenesis across various cancer types.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2627-2636"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional Characterization of RseC in the SoxR Reducing System and Its Role in Oxidative Stress Response in Escherichia coli. RseC在SoxR还原系统中的功能表征及其在大肠杆菌氧化应激反应中的作用
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-25 DOI: 10.4014/jmb.2410.10007
Kang-Lok Lee, Joon-Hee Lee, Yun-Hee Kim, Jung-Hye Roe
{"title":"Functional Characterization of RseC in the SoxR Reducing System and Its Role in Oxidative Stress Response in <i>Escherichia coli</i>.","authors":"Kang-Lok Lee, Joon-Hee Lee, Yun-Hee Kim, Jung-Hye Roe","doi":"10.4014/jmb.2410.10007","DOIUrl":"10.4014/jmb.2410.10007","url":null,"abstract":"<p><p>The reducing system of SoxR consists of a putative electron transfer system encoded by the <i>rsxABCDGE</i> operon, RseC encoded from the unlinked <i>rpoE-rseABC</i> operon, and ApbE. RseC is composed of two transmembrane helices, with both the N-terminal and C-terminal domains located in the cytoplasm. The N-terminal domain has a four-cysteine motif, CX<sub>5</sub>CX<sub>2</sub>CX<sub>5</sub>C, in the cytoplasm, with the latter three cysteines highly conserved in RseC homologs, allowing the SoxR reducer complex to function in <i>Escherichia coli</i>. These three cysteines can form an oxygen-sensitive Fe-S cluster when only the N-terminal domain is expressed in a truncated form. Without the C-terminal domain, RseC shows no significant difference in interaction with the SoxR reducer complex, but its ability to complement the function of an <i>rseC</i> mutant is greatly reduced. Additionally, the <i>rseC</i> mutant exhibits weak resistance to cumene hydrogen peroxide in the stationary phase and increased sensitivity to hydrogen peroxide in the exponential phase, independent of the SoxR regulon. This suggests that the full-length sequence of RseC is essential for its function and that it may have SoxR-independent additional roles.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2547-2554"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ischemic Stroke Induces ROS Accumulation, Maladaptive Mitophagy, and Neuronal Apoptosis in Minipigs. 缺血性脑卒中诱导小猪ROS积累、线粒体自噬不良和神经元凋亡。
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-14 DOI: 10.4014/jmb.2409.09003
Jie Chen, Yanan Bie, Yajin Guan, Wen Liu, Fei Xu, Tianping Liu, Zilong Meng, Mengqi Gao, Jiawei Liu, Shuilin Xie, Weiwang Gu
{"title":"Ischemic Stroke Induces ROS Accumulation, Maladaptive Mitophagy, and Neuronal Apoptosis in Minipigs.","authors":"Jie Chen, Yanan Bie, Yajin Guan, Wen Liu, Fei Xu, Tianping Liu, Zilong Meng, Mengqi Gao, Jiawei Liu, Shuilin Xie, Weiwang Gu","doi":"10.4014/jmb.2409.09003","DOIUrl":"10.4014/jmb.2409.09003","url":null,"abstract":"<p><p>Reactive oxygen species (ROS)-induced adaptive/maladaptive mitophagy plays an essential role in the pathophysiology of acute ischemic stroke (AIS). However, most studies have been conducted using rodent models, which limits their clinical application. In this study, we aimed to develop porcine models of permanent stroke and observe the pathophysiological alterations caused by acute ischemic stroke, focusing on ROS-induced mitophagy. Miniature pigs were subjected to lateral frontotemporal electrocoagulation, which resulted in permanent middle cerebral artery occlusion. We investigated global brain damage and mechanisms of adaptive/maladaptive mitophagy caused by ROS and global brain inflammation after AIS. An early neuroinflammatory response was observed in the ipsilateral hemisphere. ROS levels were significantly elevated in the ipsilateral hemisphere and slightly elevated in the contralateral hemisphere. ROS accumulation may be attributed to the increased production and impaired elimination of ROS. In addition, mitophagy and apoptosis were detected in the ischemic core, which may be attributed to ROS accumulation. We propose \"distinct-area targeting\" interventions aimed at maladaptive mitophagy within the ischemic core of the infarct hemisphere, which may provide new therapeutic targets for the treatment of AIS.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2648-2661"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dihydromyricetin Alleviates Non-Alcoholic Fatty Liver Disease by Modulating Gut Microbiota and Inflammatory Signaling Pathways. 二氢杨梅素通过调节肠道菌群和炎症信号通路减轻非酒精性脂肪肝。
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-20 DOI: 10.4014/jmb.2406.06048
Limin Kang, Xiaolong Ma, Fakun Yu, Lei Xu, Li Lang
{"title":"Dihydromyricetin Alleviates Non-Alcoholic Fatty Liver Disease by Modulating Gut Microbiota and Inflammatory Signaling Pathways.","authors":"Limin Kang, Xiaolong Ma, Fakun Yu, Lei Xu, Li Lang","doi":"10.4014/jmb.2406.06048","DOIUrl":"10.4014/jmb.2406.06048","url":null,"abstract":"<p><p>Non-alcoholic fatty liver disease (NAFLD) is a prevalent chronic liver condition that is strongly linked to gut microbiota imbalance and chronic inflammation. This study aims to explore the preventive effects of dihydromyricetin (DHM) on NAFLD by modulating the intestinal flora and the TLR4/NF-κB signaling pathway. Fifty male C57BL/6J mice were randomly assigned to five groups: a normal control group, a model group, and three DHM treatment groups receiving low (500 mg/kg), medium (750 mg/kg), and high doses (1,000 mg/kg). NAFLD was induced using a high-fat diet, and DHM was administered for 8 weeks. ELISA measured serum levels of LPS, IL-1β, and TNF-α, while Western Blot assessed liver expression of TLR4 and NF-κB p65. Changes in intestinal flora composition were analyzed using high-throughput 16S rRNA sequencing. The results showed that DHM treatment significantly reduced serum levels of LPS, IL-1β, and TNF-α, decreasing the liver expression of TLR4 and NF-κB p65. Intestinal flora analysis indicated a notable increase in beneficial bacteria, especially in the medium and high-dose groups. DHM treatment also significantly improved liver pathology, reducing fat deposition and inflammatory cell infiltration. In conclusion, DHM effectively prevents the progression of NAFLD by improving gut microbiota balance and suppressing inflammatory signaling pathways.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2637-2647"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-Inflammatory Activity of Biotransformed Platycodon grandiflorum Root Extracts Containing 3-O-β-D-Glucopyranosyl Platycosides in LPS-Stimulated Alveolar Macrophages, NR8383 Cells. 含 3-O-β-D-Glucopyranosyl Platycosides 的生物转化桔梗根提取物在 LPS 刺激的肺泡巨噬细胞 NR8383 细胞中的抗炎活性
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-31 DOI: 10.4014/jmb.2408.08005
Jeong Won Choi, Hyeok Jin Choi, Chae Sun Na, Hwan Lee, Byung Joo Lee, Kyung-Chul Shin, Jin Boo Jeong
{"title":"Anti-Inflammatory Activity of Biotransformed <i>Platycodon grandiflorum</i> Root Extracts Containing 3-<i>O</i>-β-D-Glucopyranosyl Platycosides in LPS-Stimulated Alveolar Macrophages, NR8383 Cells.","authors":"Jeong Won Choi, Hyeok Jin Choi, Chae Sun Na, Hwan Lee, Byung Joo Lee, Kyung-Chul Shin, Jin Boo Jeong","doi":"10.4014/jmb.2408.08005","DOIUrl":"10.4014/jmb.2408.08005","url":null,"abstract":"<p><p>Acute lung injury (ALI) is a severe inflammatory condition characterized by excessive immune responses and oxidative stress, leading to significant tissue damage. Given the need for novel therapeutic agents, this study aimed to explore the anti-inflammatory activity and mechanisms of biotransformed <i>Platycodon grandiflorum</i> root extracts (BT-PGR), which were enzymatically processed using rapidsase PL Classic from <i>Aspergillus niger</i>. The goal was to assess the potential of BT-PGR as a natural treatment for ALI. BT-PGR effectively inhibited the production of NO, iNOS, IL-1β, IL-6, and TNF-α induced by LPS in NR8383 cells. BT-PGR inhibited the phosphorylation of ERK1/2, p38, JNK and p65 in LPS-stimulated NR8383 cells. In addition, BT-PGR suppressed LPS-mediated activation of NF-κB luciferase activity. BT-PGR increased the levels of HO-1 and the inhibition of HO-1 by ZnPP attenuated BT-PGR-mediated inhibition of NO production. In addition, the inhibition of PI3K by LY294002 blocked the BT-PGR-mediated increase of HO-1 level. BT-PGR increased nuclear Nrf2 level and the knockdown of Nrf2 by siRNA inhibited BT-PGR-mediated increase of HO-1 level. In addition, inhibition of PI3K by LY294002 suppressed the increase of nuclear Nrf2 level. Based on these results, it can be inferred that BT-PGR exhibits anti-inflammatory activity in rat alveolar macrophages, suggesting its potential as a natural candidate for the improvement of ALI.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2609-2617"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Comprehensive Review of Naringenin, a Promising Phytochemical with Therapeutic Potential. 柚皮苷--一种具有治疗潜力的植物化学物质--综述。
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-22 DOI: 10.4014/jmb.2410.10006
Jun Hong Shin, Seung Ho Shin
{"title":"A Comprehensive Review of Naringenin, a Promising Phytochemical with Therapeutic Potential.","authors":"Jun Hong Shin, Seung Ho Shin","doi":"10.4014/jmb.2410.10006","DOIUrl":"10.4014/jmb.2410.10006","url":null,"abstract":"<p><p>Disorders, including cancer, metabolic disorders, and neurodegenerative diseases, can threaten human health; therefore, disease prevention is essential. Naringenin, a phytochemical with low toxicity, has been used in various disease prevention studies. This study aimed to comprehensively review the effects of naringenin on human health. First, we introduced the general characteristics of naringenin and its pharmacokinetic features when absorbed in the body. Next, we summarized the inhibitory effects of naringenin on colorectal, gastric, lung, breast, ovarian, cervical, prostate, bladder, liver, pancreatic, and skin cancers in preclinical studies. Lastly, we investigated the inhibitory effects of naringenin on metabolic disorders, including diabetes, obesity, hyperlipidemia, hypertension, cardiac toxicity, hypertrophy, steatosis, liver disease, and arteriosclerosis, as well as on neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. In conclusion, naringenin may serve as a significant natural compound that benefits human health.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2425-2438"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential Impact of Spike Protein Mutations on SARS-CoV-2 Infectivity and Immune Evasion: Insights from Delta and Kappa Variants. 刺突蛋白突变对SARS-CoV-2传染性和免疫逃避的差异影响:来自Delta和Kappa变异的见解
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-12-02 DOI: 10.4014/jmb.2411.11001
Tae-Hun Kim, Sojung Bae, Jinjong Myoung
{"title":"Differential Impact of Spike Protein Mutations on SARS-CoV-2 Infectivity and Immune Evasion: Insights from Delta and Kappa Variants.","authors":"Tae-Hun Kim, Sojung Bae, Jinjong Myoung","doi":"10.4014/jmb.2411.11001","DOIUrl":"10.4014/jmb.2411.11001","url":null,"abstract":"<p><p>SARS-CoV-2 continues to pose a global health challenge due to its high transmissibility and mutability, with new variants emerging that potentially undermine vaccination and therapeutic efforts. Mutations in the spike protein, particularly in the receptor-binding domain (RBD), significantly influence viral transmissibility and immune escape. However, the complex interplay of these mutations and their combined effects on viral fitness remain to be analyzed. In this study, we investigated the functional impact of key mutations found in the Delta and Kappa variants of SARS-CoV-2. Using pseudovirus assays, we demonstrated that the T478K and L452R mutations characteristic of the Delta variant primarily enhance viral infectivity, with minimal effect on antibody-mediated neutralization. Conversely, the E484Q mutation of the Kappa variant, alone or in combination with L452R, significantly improved evasion of antibody-mediated neutralization but appeared to compromise viral fitness and infectivity. Notably, contrary to previous reports, we found that the P681R mutation contributed neither to increased infectivity nor immune evasion at least in the assay system employed in this study. Our findings suggest that the Delta variant's global dominance over the Kappa variant may be attributed to its superior infectivity and transmissibility rather than enhanced immune evasion capabilities. These results provide valuable insights into the functional consequences of spike protein mutations and may aid in predicting the emergence and spread of future SARS-CoV-2 variants. Such understanding is crucial for enhancing public health preparedness and informing the development of next-generation vaccines and therapeutics.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2506-2515"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamic Changes in Microbiome and Metabolome during Sun-Drying of Oysters (Crassostrea gigas), a Traditional Procedure in South China. 中国南方传统牡蛎晒干过程中微生物组和代谢组的动态变化
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-07-17 DOI: 10.4014/jmb.2312.12033
Jiannan Liu, Mingjia Yu, Xiaobo Wang, Ming Qi, Minfu Wu
{"title":"Dynamic Changes in Microbiome and Metabolome during Sun-Drying of Oysters (<i>Crassostrea gigas</i>), a Traditional Procedure in South China.","authors":"Jiannan Liu, Mingjia Yu, Xiaobo Wang, Ming Qi, Minfu Wu","doi":"10.4014/jmb.2312.12033","DOIUrl":"10.4014/jmb.2312.12033","url":null,"abstract":"<p><p>Sun-drying constitutes a traditional method employed in the preparation of dried oysters within the coastal regions of South China. However, its ramifications on nutritional attributes and the genesis of flavor-contributory compounds in the resultant dried oysters remain significantly unexplored. This research endeavors to scrutinize the repercussions of the production process on the microbiota and metabolites within dried oysters. Utilizing 16s rRNA amplicon sequencing, the identification of 409 operational taxonomic units (OTUs) ensued, wherein <i>Proteobacteria</i>, <i>Bacteroidetes</i>, <i>Firmicutes</i>, <i>Tenericutes</i>, and <i>Actinobacteria</i> surfaced as the primary pathogenic bacteria present in oyster samples. Analysis of the dried oyster sample metabolomes via LC-MS unveiled a discernible augmentation in compounds associated with steroid hormone biosynthesis, arachidonic acid metabolism, biosynthesis of unsaturated fatty acids, and linoleic acid metabolism throughout the entirety of the drying process. Subsequent exploration into the association between metabolites and bacterial communities highlighted the prevailing coexistence of <i>Mycoplasma</i>, <i>Psychrilyobacter</i>, and <i>Vibrio</i> demonstrating negative correlations with a substantial number of metabolites across categories including organic acid and its derivatives, nucleotide and its metabolites, free fatty acids, and amino acids. Conversely, <i>Shewanella</i> and <i>Arcobacter</i> exhibited positive correlations with these metabolite categories. This exhaustive investigation offers invaluable insights into the dynamic alterations within the microbiota and metabolites of dried oysters across diverse drying periods. These findings are anticipated to significantly contribute to the advancement of production techniques and the formulation of enhanced safety measures for the processing of dried oysters.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2555-2564"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Endophytic Streptomyces rhizosphaericola Ahn75 and Its Potential for Biocontrol against Rice Blast. 根瘤链霉菌 Ahn75 的内生特性及其对水稻纹枯病的生物防治潜力。
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-22 DOI: 10.4014/jmb.2407.07018
Zujiao Fu, Rong Xiao, Zhan Hu, Min Zhang, Shandong Wu, Zhaohui Guo, Rongjun Luo, Shiping Shan, Hua Yang
{"title":"Characterization of Endophytic <i>Streptomyces rhizosphaericola</i> Ahn75 and Its Potential for Biocontrol against Rice Blast.","authors":"Zujiao Fu, Rong Xiao, Zhan Hu, Min Zhang, Shandong Wu, Zhaohui Guo, Rongjun Luo, Shiping Shan, Hua Yang","doi":"10.4014/jmb.2407.07018","DOIUrl":"10.4014/jmb.2407.07018","url":null,"abstract":"<p><p>Plant endophyte Streptomyces are excellent candidates as biocontrol agents against the rice blast fungus, <i>Magnaporthe oryzae</i>. In this study, a novel strain Ahn75 with antifungal activity was isolated from healthy rice stem and identified as <i>Streptomyces rhizosphaericola</i> by phenotypic characterization and phylogenetic analysis based on 16S rRNA gene, multilocus and genome sequences. Inhibition test using culture filtrate showed that Ahn75 could effectively suppress <i>M. oryzae</i>, with mycelia growth inhibition rate of 80.88% and spore germination inhibition rate of 78.26%. Genome sequence analysis of strain Ahn75 showed 40 gene clusters of secondary metabolites and several genes related to plant growth promotion were predicted in the genome of Ahn75. Several antimicrobial compounds including valinomycin, tetrabutylammonium, and benzalkonium chloride, were also detected in the antifungal fraction from Ahn75 culture filtrate by liquid chromatography and high resolution mass spectrometry. Meanwhile, strain Ahn75 demonstrates UV tolerance under UV irradiation for 60 min, pH tolerance between pH6 and pH9, and a high halotolerance in 7% (w/v) of NaCl. Greenhouse experiments indicated that Ahn75 is able to colonize rice stems, roots, and leaves, which help rice to reduce the rice leaf blast incidence by 59.76%. All these findings suggest that strain Ahn75 could be a potential biocontrol agent for rice blast.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2516-2526"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Innovative Bacterial Therapies and Genetic Engineering Approaches in Colorectal Cancer: A Review of Emerging Strategies and Clinical Implications. 大肠癌的创新细菌疗法和基因工程方法:新策略和临床意义综述》。
IF 2.5 4区 生物学
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-09-25 DOI: 10.4014/jmb.2408.08026
Chunxiao Song, Chunwu Zhao
{"title":"Innovative Bacterial Therapies and Genetic Engineering Approaches in Colorectal Cancer: A Review of Emerging Strategies and Clinical Implications.","authors":"Chunxiao Song, Chunwu Zhao","doi":"10.4014/jmb.2408.08026","DOIUrl":"10.4014/jmb.2408.08026","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is considered a widespread cancer, ranking second in mortality and incidence among cancer patients worldwide. CRC develops from adenoma to carcinoma through the dynamic interplay of genetic and environmental factors. The conventional modes of treatment, including operation, chemotherapy, and irradiation, are associated with significant challenges, such as drug resistance and toxicity, necessitating the exploration of new treatment modalities. These difficulties reveal the necessity of the emergence of new therapeutic approaches. This review mainly emphasizes the bacterial-based therapies that have recently developed like the engineered bacteriophage therapy and bacterial immunotherapy that pale the existing chemotherapy in terms of toxicity but are effective in killing tumor cells. Also, it also investigates various molecular genetic engineering strategies such as CRISPR-Cas9, CRISPR prime editing and gene silencing to achieve better targeting of CRC. Implementing these new approaches into the forefront of CRC treatment may bring better, more effective therapy with fewer side effects on patients' quality of life.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2397-2412"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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