{"title":"Antidepressant Effect and Mechanism of <i>Picea mariana</i> Essential Oil on ReserpineInduced Depression Model Mice.","authors":"Ying Wang, Guofeng Shi, Yixi Zeng, Juting Li, Yongyu Wu, Jiahui Zheng, Anjing Xu, Yanqing Ma, Lanyue Zhang, Hui Li","doi":"10.4014/jmb.2405.05013","DOIUrl":"https://doi.org/10.4014/jmb.2405.05013","url":null,"abstract":"<p><p>The disturbance of brain biochemical substances serves as a primary cause and aggravating factor of depression. This study aimed to investigate the principal components of Picea mariana and its effect on reserpine-induced depression mice,w ith its relationship with brain central transmitters and related proteins. Methods: The main constituents of <i>Picea mariana</i> essential oil (PMEO) were analyzed by GC-MS spectrometry. The quiescent time in the tail suspension test (TST) and forced swim test (FST), along with the weight change of the mice was detected. The number of normal neurons was quantified through Nissl staining. Immunohistochemistry was employed to determine the levels of 5HT-<sub>1A</sub> and 5HT-<sub>2A</sub> in the brain. Western blotting was utilized to detect 5HT-<sub>2A</sub>, CRF and TrkB protein levels. RTqPCR was used to detect the mRNA levels of 5HT-<sub>1A</sub>, 5HT-<sub>2A</sub>, TrkB, CRF, and BDNF. The main active ingredients of PMEOs were (-) -bornyl acetate (44.95%), γ-Terpinene (14.17%), and β-Pinene (10.12%). PMEOs effectively improved the retardation and weight loss due to anorexia in depression-like mice. This improvement was associated with an increase in the number of normal neurons. After administering different doses of PMEOs, the levels of 5HT-<sub>1A</sub>, 5HT-<sub>2A</sub>, CRF, and TrkB were found to be increased in brain tissue. RT-qPCR revealed that the mRNA levels of CRF, 5HT-<sub>1A</sub>, and 5HT-<sub>2A</sub> were generally upregulated, whereas TrkB and BDNF were downregulated. Conclusion: PMEO can effectively alleviate depression induced by reserpine, which may be attributed to its regulation of 5HT-<sub>1A</sub>, 5HT-<sub>2A</sub>, CRF and TrkB protein expression, thus reducing brain nerve injury.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Developing Strain-Specific Simple Sequence Repeat (SSR) Markers for <i>Chlorella sorokiniana</i>.","authors":"Mais Sweiss, Maen Hasan, Nidal Odat","doi":"10.4014/jmb.2404.04024","DOIUrl":"https://doi.org/10.4014/jmb.2404.04024","url":null,"abstract":"<p><p><i>Chlorella sorokiniana</i> green microalga offers many environmentally friendly applications, including wastewater treatment, biofertilizers, animal feed, and biofuel production. Different strains of <i>C. sorokiniana</i> have unique properties that may suit one application but not another. There is a need to distinguish between the many available strains of <i>C. sorokiniana</i> to choose the one that best fits the application. Consequently, our research goal was to develop strain-specific simple sequence repeat (SSR) markers to differentiate between the different strains. Seventeen markers spanning ten out of the twelve chromosomes of the <i>C. sorokiniana</i> genome were developed and validated on eight different strains from culture collections and our lab, and were then analyzed by fragment analysis. The results demonstrate the potential of these polymorphic markers to detect the genetic differences between the strains of <i>C.</i> <i>sorokiniana</i>, and to serve as useful tools for the intra-species population genetic analysis and conservation genetics studies of <i>C. sorokiniana.</i></p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hend Algadi, Mohammed Abdelfatah Alhoot, Anis Rageh Al-Maleki, Neny Purwitasari
{"title":"Effects of Metal and Metal Oxide Nanoparticles against Biofilm-Forming Bacteria: A systematic Review.","authors":"Hend Algadi, Mohammed Abdelfatah Alhoot, Anis Rageh Al-Maleki, Neny Purwitasari","doi":"10.4014/jmb.2403.03029","DOIUrl":"https://doi.org/10.4014/jmb.2403.03029","url":null,"abstract":"<p><p>Biofilm formation by bacteria poses a significant challenge across diverse industries, displaying resilience against conventional antimicrobial agents. Nanoparticles emerge as a promising alternative for addressing biofilm-related issues. This review aims to assess the efficacy of metal and metal oxide nanoparticles in inhibiting or disrupting biofilm formation by various bacterial species. It delineates trends, identifies gaps, and outlines avenues for future research, emphasizing best practices and optimal nanoparticles for biofilm prevention and eradication. Additionally, it underscores the potential of nanoparticles as substitutes for traditional antibiotics in healthcare and combating antibiotic resistance. A systematic literature search, encompassing Web of Science, PubMed, and Google Scholar from 2015 to 2023, yielded 48 publications meeting the review criteria. These studies employed diverse methods to explore the antibacterial activity of nanoparticles against biofilmforming bacteria strains. The implications of this study are profound, offering prospects for novel antimicrobial agents targeting biofilm-forming bacteria, often resistant to conventional antibiotics. In conclusion, nanoparticles present a promising frontier in countering biofilm-forming bacteria. This review delivers a structured analysis of current research, providing insights into the potential and challenges of nanoparticle utilization against biofilm-related challenges. While nanoparticles exhibit inherent antimicrobial properties with applications spanning healthcare, agriculture, and industries, the review acknowledges limitations such as the narrow scope of tested nanoparticles and the imperative need for extensive research on long-term toxicity and environmental impacts.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Man Lu, Liwei Xie, Sijie Yin, Jing Zhou, Lingmei Yi, Ling Ye
{"title":"The Gut Microbial Lipid Metabolite 14(15)- EpETE Inhibits Substance P Release by Targeting GCG/PKA Signaling to Relieve Cisplatin-Induced Nausea and Vomiting in Rats.","authors":"Man Lu, Liwei Xie, Sijie Yin, Jing Zhou, Lingmei Yi, Ling Ye","doi":"10.4014/jmb.2403.03044","DOIUrl":"https://doi.org/10.4014/jmb.2403.03044","url":null,"abstract":"<p><p>Chemotherapy-induced nausea and vomiting (CINV) is a debilitating side effect related to activation of substance P (SP). SP activation can result from dysregulation of the gut-brain axis, and also from activation of protein kinase A signaling (PKA) signaling. In this study, we connected these factors in an attempt to unveil the mechanisms underlying CINV and develop new therapeutic strategies. Female rats were injected with cisplatin to induce pica. Fecal samples were collected before/after injection, and subjected to lipid metabolomics analysis. In another portion of pica rats, the PKA inhibitor KT5720 was applied to investigate the involvement of PKA signaling in CINV, while fecal microbiota transplantation (FMT) was implemented to verify the therapeutic effect of the lipid metabolite 14(15)-EpETE. Pica symptoms were recorded, followed by ileal histological examination. The targeting relationship between 14(15)-EpETE and glucagon was determined by bioinformatics. SP and glucagon/PKA signaling in rat ileum, serum, and/or brain substantia nigra were detected by immunohistochemistry, enzyme-linked immunosorbent assay, and/or western blot. The results showed a significantly lower level of 14(15)-EpETE in rat feces after cisplatin injection. KT5720 treatment alleviated cisplatin-induced pica symptoms, ileal injury, SP content increase in the ileum, serum, and brain substantia nigra, and ileal PKA activation in rats. The ileal level of glucagon was elevated by cisplatin in rats. FMT exerted an effect similar to that of KT5720 treatment, relieving the cisplatin-induced changes, including ileal glucagon/PKA activation in rats. Our findings demonstrate that FMT restores 14(15)-EpETE production, which inhibits SP release by targeting GCG/PKA signaling, ultimately mitigating CINV.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huynh Thi Ngoc Mi, Heji Kim, Jong Suk Lee, Bekir Engin Eser, Jaehong Han
{"title":"Flavonoids Biotransformation by Human Gut Bacterium <i>Dorea</i> sp. MRG-IFC3 Cell-Free Extract.","authors":"Huynh Thi Ngoc Mi, Heji Kim, Jong Suk Lee, Bekir Engin Eser, Jaehong Han","doi":"10.4014/jmb.2403.03058","DOIUrl":"10.4014/jmb.2403.03058","url":null,"abstract":"<p><p>Human gut bacterium <i>Dorea</i> sp. MRG-IFC3 is unique in that it is capable of metabolizing puerarin, an isoflavone <i>C</i>-glycoside, whereas it shows broad substrate glycosidase activity for the various flavonoid <i>O</i>-glycosides. To address the question on the substrate specificity, as well as biochemical characteristics, cell-free biotransformation of flavonoid glycosides was performed under various conditions. The results showed that there are two different enzyme systems responsible for the metabolism of flavonoid <i>C</i>-glycosides and <i>O</i>-glycosides in the MRG-IFC3 strain. The system responsible for the conversion of puerarin was inducible and comprised of two enzymes. One enzyme oxidizes puerarin to 3\"-oxo-puerarin and the other enzyme converts 3\"-oxo-puearin to daidzein. The second enzyme was only active toward 3\"-oxo-puerarin. The activity of puerarin conversion to daidzein was enhanced in the presence of Mn<sup>2+</sup> and NAD<sup>+</sup>. It was concluded that the puerarin <i>C</i>-deglycosylation by <i>Dorea</i> sp. MRG-IFC3 possibly adopts the same biochemical mechanism as the strain PUE, a species of <i>Dorea longicatena</i>.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanyan Song, Jing Zhang, Yidan Li, Yuxuan Wang, Yingxin Wan
{"title":"Potential Protective Effect of Selenium-Enriched <i>Lactobacillus plantarum</i> on Cadmium-Induced Liver Injury in Mice.","authors":"Yanyan Song, Jing Zhang, Yidan Li, Yuxuan Wang, Yingxin Wan","doi":"10.4014/jmb.2312.12051","DOIUrl":"10.4014/jmb.2312.12051","url":null,"abstract":"<p><p>Cadmium (Cd) is a prevalent environmental contaminant that poses a potential hazard to the health of both humans and animals. In this study, biosynthesized selenium-enriched <i>Lactobacillus plantarum</i> and selenium nanoparticles (SeNPs) were developed and evaluated for their protective effects against Cd-induced hepatic injury in mice through oral administration for 4 weeks. Cadmium exposure resulted in severe impairment of liver function, as evidenced by increased levels of serum markers of liver injury and, oxidative stress and significant damage to liver tissue, and a notable decrease in the diversity of the intestinal microbiota. Oral administration of Se-enriched <i>L. plantarum</i> (LS) reduced cadmium accumulation in the liver by 49.5% and, restored other cadmium-induced damage markers to normal levels. A comparison of the effects with those of <i>L. plantarum</i> (L) and SeNPs isolated from LS revealed that LS could more effectively alleviate hepatic oxidative stress and reduce the intrahepatic inflammatory responses of the liver, further protecting against cadmium-induced liver injury. These findings suggest that the development of LS may be effective at protecting the liver and intestinal tract from cadmium-induced damage.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hao Huang, Rui Han, Ping-Ping Huang, Chuan-Yue Qiao, Shuang Bian, Han Xiao, Lei Ma
{"title":"Preparation and Performance Evaluation of a Zinc Oxide-Graphene Oxideloaded Chitosan-Based Thermosensitive Gel.","authors":"Hao Huang, Rui Han, Ping-Ping Huang, Chuan-Yue Qiao, Shuang Bian, Han Xiao, Lei Ma","doi":"10.4014/jmb.2402.02055","DOIUrl":"10.4014/jmb.2402.02055","url":null,"abstract":"<p><p>This study aimed to develop and assess a chitosan biomedical antibacterial gel ZincOxide-GrapheneOxide/Chitosan/β-Glycerophosphate (ZnO-GO/CS/β-GP) loaded with nano-zinc oxide (ZnO) and graphene oxide (GO), known for its potent antibacterial properties, biocompatibility, and sustained drug release. ZnO nanoparticles (ZnO-NPs) were modified and integrated with GO sheets to create 1% and 3% ZnO-GO/CS/β-GP thermo-sensitive hydrogels based on ZnO-GO to Chitosan (CS) mass ratio. Gelation time, pH, structural changes, and microscopic morphology were evaluated. The hydrogel's antibacterial efficacy against Porphyromonas gingivalis, biofilm biomass, and metabolic activity was examined alongside its impact (MC3T3-e1). The findings of this study revealed that both hydrogel formulations exhibited temperature sensitivity, maintaining a neutral pH. The ZnO-GO/CS/β-GP formulation effectively inhibited P. gingivalis bacterial activity and biofilm formation, with a 3% ZnO-GO/CS/β-GP antibacterial rate approaching 100%. MC3T3-e1 cells displayed good biocompatibility when cultured in the hydrogel extract.The ZnO-GO/CS/β-GP thermo-sensitive hydrogel demonstrates favorable physical and chemical properties, effectively preventing <i>P. gingivalis</i> biofilm formation. It exhibits promising biocompatibility, suggesting its potential as an adjuvant therapy for managing and preventing peri-implantitis, subject to further clinical investigations.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanchao Liu, Hui Pang, Na Li, Yang Jiao, Zexu Zhang, Qin Zhu
{"title":"The Metabolic Functional Feature of Gut Microbiota in Mongolian Patients with Type 2 Diabetes.","authors":"Yanchao Liu, Hui Pang, Na Li, Yang Jiao, Zexu Zhang, Qin Zhu","doi":"10.4014/jmb.2402.02021","DOIUrl":"10.4014/jmb.2402.02021","url":null,"abstract":"<p><p>The accumulating evidence substantiates the indispensable role of gut microbiota in modulating the pathogenesis of type 2 diabetes. Uncovering the intricacies of the mechanism is imperative in aiding disease control efforts. Revealing key bacterial species, their metabolites and/or metabolic pathways from the vast array of gut microorganisms can significantly contribute to precise treatment of the disease. With a high prevalence of type 2 diabetes in Inner Mongolia, China, we recruited volunteers from among the Mongolian population to investigate the relationship between gut microbiota and the disease. Fecal samples were collected from the Volunteers of Mongolia with Type 2 Diabetes group and a Control group, and detected by metagenomic analysis and untargeted metabolomics analysis. The findings suggest that <i>Firmicutes</i> and <i>Bacteroidetes</i> phyla are the predominant gut microorganisms that exert significant influence on the pathogenesis of type 2 diabetes in the Mongolian population. In the disease group, despite an increase in the quantity of most gut microbial metabolic enzymes, there was a concomitant weakening of gut metabolic function, suggesting that the gut microbiota may be in a compensatory state during the disease stage. β-Tocotrienol may serve as a pivotal gut metabolite produced by gut microorganisms and a potential biomarker for type 2 diabetes. The metabolic biosynthesis pathways of ubiquinone and other terpenoid quinones could be the crucial mechanism through which the gut microbiota regulates type 2 diabetes. Additionally, certain <i>Clostridium</i> gut species may play a pivotal role in the progression of the disease.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141087713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Progress on Strategies for Improving the Enzyme Properties of Bacteriophage Endolysins.","authors":"Yulu Wang, Xue Wang, Xin Liu, Bokun Lin","doi":"10.4014/jmb.2312.12050","DOIUrl":"10.4014/jmb.2312.12050","url":null,"abstract":"<p><p>Bacterial resistance to commonly used antibiotics is one of the major challenges to be solved today. Bacteriophage endolysins (Lysins) have become a hot research topic as a new class of antibacterial agents. They have promising applications in bacterial infection prevention and control in multiple fields, such as livestock and poultry farming, food safety, clinical medicine and pathogen detection. However, many phage endolysins display low bactericidal activities, short half-life and narrow lytic spectrums. Therefore, some methods have been used to improve the enzyme properties (bactericidal activity, lysis spectrum, stability and targeting the substrate, etc) of bacteriophage endolysins, including deletion or addition of domains, DNA mutagenesis, chimerization of domains, fusion to the membrane-penetrating peptides, fusion with domains targeting outer membrane transport systems, encapsulation, the usage of outer membrane permeabilizers. In this review, research progress on the strategies for improving their enzyme properties are systematically presented, with a view to provide references for the development of lysins with excellent performances.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sang-Gyu Kim, Bo-Eun Kim, Jung Hun Lee, Dae-Wi Kim
{"title":"Novel Qnr Families as Conserved and Intrinsic Quinolone Resistance Determinants in <i>Aeromonas</i> spp.","authors":"Sang-Gyu Kim, Bo-Eun Kim, Jung Hun Lee, Dae-Wi Kim","doi":"10.4014/jmb.2403.03043","DOIUrl":"10.4014/jmb.2403.03043","url":null,"abstract":"<p><p>The environment has been identified as an origin, reservoir, and transmission route of antibiotic resistance genes (ARGs). Among diverse environments, freshwater environments have been recognized as pivotal in the transmission of ARGs between opportunistic pathogens and autochthonous bacteria such as <i>Aeromonas</i> spp. In this study, five environmental strains of <i>Aeromonas</i> spp. exhibiting multidrug resistance (MDR) were selected for whole-genome sequencing to ascertain their taxonomic assignment at the species-level and to delineate their ARG repertoires. Analyses of their genomes revealed the presence of one protein almost identical to AhQnr (<i>A. hydrophila</i> Qnr protein) and four novel proteins similar to AhQnr. To scrutinize the classification and taxonomic distribution of these proteins, all <i>Aeromonas</i> genomes deposited in the NCBI RefSeq genome database (1,222 genomes) were investigated. This revealed that these <i>Aeromonas</i> Qnr (AQnr) proteins are conserved intrinsic resistance determinants of the genus, exhibiting species-specific diversity. Additionally, structure prediction and analysis of contribution to quinolone resistance by AQnr proteins of the isolates, confirmed their functionality as quinolone resistance determinants. Given the origin of mobile <i>qnr</i> genes from aquatic bacteria and the crucial role of <i>Aeromonas</i> spp. in ARG dissemination in aquatic environments, a thorough understanding and strict surveillance of AQnr families prior to the clinical emergence are imperative. In this study, using comparative genome analyses and functional characterization of AQnr proteins in the genus <i>Aeromonas</i>, novel <i>Aeromonas</i> ARGs requiring surveillance has suggested.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140870481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}