Journal of microbiology and biotechnology最新文献_第8页

Production of Vitamin B₁₂ in Escherichia coli Using a Thermal Switch to Control Pathway Genes. 利用热敏开关控制途径基因在大肠杆菌中产生维生素B12。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-11 DOI: 10.4014/jmb.2412.12068

Kaize Kong, Feitao Chen, Huan Fang, Pingtao Jiang, Xinfang Zhao, Jijiao Zhang, Huina Dong, Hongxing Jin, Dawei Zhang

{"title":"Production of Vitamin B12 in Escherichia coli Using a Thermal Switch to Control Pathway Genes.","authors":"Kaize Kong, Feitao Chen, Huan Fang, Pingtao Jiang, Xinfang Zhao, Jijiao Zhang, Huina Dong, Hongxing Jin, Dawei Zhang","doi":"10.4014/jmb.2412.12068","DOIUrl":"https://doi.org/10.4014/jmb.2412.12068","url":null,"abstract":"Isopropyl β-D-thiogalactopyranoside (IPTG), while widely utilized for inducing gene expression in systems governed by T7lac and related promoters, poses significant challenges due to its toxicity and expense, prompting the exploration of alternative induction strategies. In this study, we developed a series of inducer-free vitamin B12-producing strains featuring thermally regulated pathway genes. We engineered a thermal switch by replacing the lacI promoter with the PR promoter, which is regulated by the temperature-sensitive repressor cI857 from the λ bacteriophage. As a result, target genes driven by T7lac or other lac-derived promoters containing lac operators were expressed upon lowering the temperature. Our findings indicate that culturing at 37°C and then shifting to 32°C when the optical density at 600 nm reaches 2 is the most effective strategy for vitamin B12 production. Additionally, the vitamin B12 titer increased by 37.96% after introducing an ssrA degradation tag at the C-terminus of lacI. This study introduces a novel strategy for vitamin B12 production that circumvents the need for IPTG by implementing a thermal switch. This approach may have significant implications for chemical bioproduction processes that have traditionally relied on IPTG for gene induction.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412068"},"PeriodicalIF":2.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of GLS1 and ASCT2 Synergistically Enhances the Anticancer Effects in Pancreatic Cancer Cells. 抑制GLS1和ASCT2协同增强胰腺癌细胞的抗癌作用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-10 DOI: 10.4014/jmb.2412.12032

Dong-Hwan Kim, Dong Joon Kim, Seong-Jun Park, Won-Jun Jang, Chul-Ho Jeong

{"title":"Inhibition of GLS1 and ASCT2 Synergistically Enhances the Anticancer Effects in Pancreatic Cancer Cells.","authors":"Dong-Hwan Kim, Dong Joon Kim, Seong-Jun Park, Won-Jun Jang, Chul-Ho Jeong","doi":"10.4014/jmb.2412.12032","DOIUrl":"https://doi.org/10.4014/jmb.2412.12032","url":null,"abstract":"Pancreatic cancer, a leading cause of cancer-related deaths, is characterized by increased dependence on glutamine metabolism. Telaglenastat (CB-839), a glutaminase (GLS) inhibitor targets glutamine metabolism; however, its efficacy as monotherapy is limited owing to metabolic adaptations. In this study, we demonstrated that CB-839 effectively inhibited cell growth in pancreatic cancer cells, but activated the general control nonderepressible 2 (GCN2)-activating transcription factor 4 (ATF4) signaling pathway. ATF4 knockdown reduced glutamine transporter alanine, serine, and cysteine transporter 2 (ASCT2) expression, glutamine uptake, and cell viability under glutamine deprivation-recovery conditions, confirming its protective role in mitigating glutamine-related metabolic stress. Notably, the combination of CB-839 and the ASCT2 inhibitor V-9302 demonstrated a synergistic effect, significantly suppressing pancreatic cancer cell survival. These findings highlight ATF4 and ASCT2 as crucial therapeutic targets and indicate that dual inhibition of GLS and ASCT2 may enhance treatment outcomes for pancreatic cancer.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412032"},"PeriodicalIF":2.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Codon Optimization Tools: Advancing toward a Multi-Criteria Framework for Synthetic Gene Design. 密码子优化工具的比较分析：迈向合成基因设计的多标准框架。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-10 DOI: 10.4014/jmb.2411.11066

Eden A Demissie, Seo-Young Park, Je Hun Moon, Dong-Yup Lee

{"title":"Comparative Analysis of Codon Optimization Tools: Advancing toward a Multi-Criteria Framework for Synthetic Gene Design.","authors":"Eden A Demissie, Seo-Young Park, Je Hun Moon, Dong-Yup Lee","doi":"10.4014/jmb.2411.11066","DOIUrl":"https://doi.org/10.4014/jmb.2411.11066","url":null,"abstract":"Codon optimization is an essential technique in synthetic biology and biopharmaceutical production, enhancing recombinant protein expression by fine-tuning genetic sequences to match the translational machinery and codon usage preferences of specific host organisms. This study presents a comprehensive comparative analysis of widely used codon optimization tools, focusing on their capacity to reflect host-specific codon biases, design principles, and parameters. Industrially relevant target proteins were evaluated in Escherichia coli, Saccharomyces cerevisiae, and CHO cells, uncovering significant variability in sequence design and clustering patterns across tools. Tools such as JCat, OPTIMIZER, ATGme, and GeneOptimizer demonstrated strong alignment with genome-wide and highly expressed gene-level codon usage, achieving high codon adaptation index (CAI) values and efficient codon-pair utilization. Conversely, tools like TISIGNER and IDT employed different optimization strategies that frequently produced divergent results. Other key parameters, including GC content, mRNA secondary structure stability (ΔG), and codon-pair bias (CPB), were analyzed to elucidate their influence on translational efficiency. While increased GC content enhanced mRNA stability in E. coli, A/T-rich codons in S. cerevisiae minimized secondary structure formation, and moderate GC content in CHO cells balanced mRNA stability and translation efficiency. Our findings highlight the limitations of single-metric approaches and advocate for a multi-criteria framework that integrates CAI, GC content, mRNA folding energy, and codon-pair considerations. This integrative strategy enables the design of tailored genetic sequences that meet host-specific requirements, advancing synthetic gene design for biotechnological innovation and precision biopharmaceutical applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411066"},"PeriodicalIF":2.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Endophytic Fungi and Effects on Secondary Metabolites in Hairy Roots of Salvia miltiorrhiza. 丹参毛状根内生真菌的分离及其对次生代谢产物的影响。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-09 DOI: 10.4014/jmb.2411.11051

Yiming Wang, Shiyu Cai, Ziling Tao, Junzhi Peng, Dan Li, Ludan Li, Xiaoying Cao, Jihong Jiang

{"title":"Isolation of Endophytic Fungi and Effects on Secondary Metabolites in Hairy Roots of Salvia miltiorrhiza.","authors":"Yiming Wang, Shiyu Cai, Ziling Tao, Junzhi Peng, Dan Li, Ludan Li, Xiaoying Cao, Jihong Jiang","doi":"10.4014/jmb.2411.11051","DOIUrl":"https://doi.org/10.4014/jmb.2411.11051","url":null,"abstract":"The slow growth rate of medicinal plants has made them unable to meet people's needs, and the use of biotechnology to obtain natural products from medicinal plants can alleviate this problem. This study isolated and identified 42 endophytic fungi from the roots, stems, and leaves of Salvia miltiorrhiza, belonging to 13 genera. The endophytic fungi that promote the accumulation of secondary metabolites in the hairy roots of S. miltiorrhiza were screened by co-culture and elicitors preparation. Among them, 15 endophytic fungi presented relatively high crude polysaccharide yields. Co-culture experiments showed that endophytic strains had different effects on the biomass and the accumulation of secondary metabolites in the hairy roots of S. miltiorrhiza, with strain KLBMPSM237 being the most effective. The contents of tanshinone I, salvianolic acid B and rosmarinic acid in the hairy roots of S. miltiorrhiza were significantly increased by KLBMPSM237 polysaccharide inducers at different concentrations. This study provides new microbial resources and technical methods for increasing the natural products in hairy roots of S. miltiorrhiza.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411051"},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Microalgal Cell Division Tendency on OD to DCW Conversion Factor and Chlorophyll Contents. 微藻细胞分裂倾向对OD - DCW转换因子和叶绿素含量的影响。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-09 DOI: 10.4014/jmb.2412.12049

Hyung Woon Choe, DanA Kim, Young Jun Choi, Hyun Gi Koh, Won-Kun Park

{"title":"Influence of Microalgal Cell Division Tendency on OD to DCW Conversion Factor and Chlorophyll Contents.","authors":"Hyung Woon Choe, DanA Kim, Young Jun Choi, Hyun Gi Koh, Won-Kun Park","doi":"10.4014/jmb.2412.12049","DOIUrl":"https://doi.org/10.4014/jmb.2412.12049","url":null,"abstract":"As the importance of achieving a carbon-neutral society grow, research on the production of microalgal bioproducts have gained significant attention. Among the production processes, cultivation has emerged as a critical step as it determines overall productivity and feasibility. However, changes in cell properties during cultivation pose challenges, and there is currently no direct method to simultaneously assess cell growth, cellular division properties and cell enlargement. In this study, two green algae (Chlorella sp. ABC001 and Chlorella sp. HS2) were cultivated under photo and mixotrophic conditions to evaluate their growth, cell size profile, and changes in optical density. The mixotrophic condition resulted in 2.1 and 1.6-fold higher biomass yields than the photoautotrophic condition for ABC001 and HS2, respectively at day 4. Over the 4-day cultivation, cell sizes ranged from 2.51 to 5.57 μm for ABC001 and from 2.56 to 4.41 μm for HS2. By analyzing changes in the conversion factor between dry cell weight (DCW) and optical density (OD), it was observed that variations in the slope correlated with changes in cell size. Additionally, chlorophyll content fluctuated during cultivation, reaching maximum levels (14.34 and 16.58 μg/mg biomass, respectively) under phototrophic condition on day 2. This study highlights the relationship between cell division tendencies, DCW, cell size, and OD, demonstrating their critical role in determining cellular component content. For future optimization of algal product production processes, further research on these cellular differentiation mechanisms will be essential.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412049"},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Marine Microorganism-Derived Oils Containing EPA and DHA for Triglyceride Improvement and Blood Circulation Enhancement. 含有EPA和DHA的海洋微生物衍生油对改善甘油三酯和促进血液循环的评价。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-09 DOI: 10.4014/jmb.2412.12009

Tae Yeon Yin, Jung-Rae Rho, Yeong Du Yoo, Eun Ju Jeong, Jaeyeon Park, Eun Young Yoon

{"title":"Evaluation of Marine Microorganism-Derived Oils Containing EPA and DHA for Triglyceride Improvement and Blood Circulation Enhancement.","authors":"Tae Yeon Yin, Jung-Rae Rho, Yeong Du Yoo, Eun Ju Jeong, Jaeyeon Park, Eun Young Yoon","doi":"10.4014/jmb.2412.12009","DOIUrl":"https://doi.org/10.4014/jmb.2412.12009","url":null,"abstract":"Cardiovascular diseases (CVDs) present significant health challenges globally, with dysregulated triglyceride levels and impaired endothelial function being key contributors to their pathogenesis. In this study, we explore the potential of marine Microorganism-derived oils rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in addressing these physiological phenomena associated with CVDs. Exploring marine resources for physiologically active compounds intertwines with ecological considerations, highlighting the interconnectedness between human health and the environment. Marine microorganisms, particularly protist Oxyrrhis, are abundant sources of bioactive compounds, including EPA, known for their lipid-lowering effects and vascular benefits. Additionally, marine biodiversity offers a rich reservoir of compounds with potential health-promoting properties. In this study, we evaluated the efficacy of EPA- and DHA-containing oils in improving triglyceride levels and enhancing blood circulation. Lipoprotein lipase (LPL) and nitric oxide (NO) were identified as pivotal players in the mechanism of action, with LPL facilitating neutral fat metabolism and NO regulating vascular tone and function. This study contributes to the growing evidence supporting the use of marine-derived compounds in preventing and managing CVD, emphasizing the ecological importance of marine ecosystems to human health by leveraging the bioactive potential of marine biodiversity sustainably.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412009"},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Probiotic Formula (Lacto-5X) on Constipation: Improvements in Gastrointestinal Symptoms, Gut Microbiome, and Metabolites. 益生菌配方（乳酸- 5x）对便秘的影响：改善胃肠道症状、肠道微生物群和代谢物。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-09 DOI: 10.4014/jmb.2412.12056

Yoo-Jeong Jin, Young Jae Park, Jieun Choi, Myung-Soo Kim, Uigi Min, Jonghyun Lim, Jooyeon Kang, Do Yup Lee, Byung-Yong Kim

{"title":"Impact of Probiotic Formula (Lacto-5X) on Constipation: Improvements in Gastrointestinal Symptoms, Gut Microbiome, and Metabolites.","authors":"Yoo-Jeong Jin, Young Jae Park, Jieun Choi, Myung-Soo Kim, Uigi Min, Jonghyun Lim, Jooyeon Kang, Do Yup Lee, Byung-Yong Kim","doi":"10.4014/jmb.2412.12056","DOIUrl":"https://doi.org/10.4014/jmb.2412.12056","url":null,"abstract":"Constipation is characterized by low frequent stools and difficult stool passage. Approximately 16% of the global population experiences these symptoms. Probiotics have shown promise in improving constipation symptoms by modulating the gut microbiome. This study aims to evaluate the effects of a probiotic formula (Lacto-5X) on bowel habits, gastrointestinal symptoms, gut microbiome, and metabolites in adults with mild constipation using a randomized, double-blind, placebo-controlled clinical trial design. At the 4-week endpoint, the Probiotic group had significant improvements in stool consistency, stool frequency, abdominal pain, and straining compared to the Placebo group. Satisfaction with bowel habits and improvement in overall intestinal health were significantly higher in the Probiotic group. Microbiome analysis revealed a significant increase in the abundance of Lactobacillus and L. plantarum in the Probiotic group at the 4-week endpoint. Metabolome analysis showed that L-proline level in the Probiotic group decreased, while threonic acid level increased at the 4-week endpoint compared to the Placebo group. However, these improvements were not sustained at the 8-week follow-up point. Lacto-5X changes the gut microbiome, leading to changes in metabolites, and it induced improved constipation symptoms. Continuous intake may be necessary to maintain these effects. Further studies are needed to explore the long-term efficacy of Lacto-5X.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412056"},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of a Bacillus amyloliquefaciens Bacteriophage JBA6 and Its Endolysin PlyJBA6. 解淀粉芽孢杆菌噬菌体JBA6及其内溶素PlyJBA6的分离与鉴定

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-04-01 DOI: 10.4014/jmb.2502.02026

Jena Kim, Minsuk Kong

{"title":"Isolation and Characterization of a Bacillus amyloliquefaciens Bacteriophage JBA6 and Its Endolysin PlyJBA6.","authors":"Jena Kim, Minsuk Kong","doi":"10.4014/jmb.2502.02026","DOIUrl":"10.4014/jmb.2502.02026","url":null,"abstract":"Bacillus amyloliquefaciens is a Gram-positive, aerobic, spore-forming bacteria usually found in soil. Despite its probiotic potential, B. amyloliquefaciens has been identified as a cause of food spoilage, including the development of off-odors, rope formation, and the production of viscous substances in a wide range of foods. To control B. amyloliquefaciens, we isolated three B. amyloliquefaciens bacteriophages TBA3, JBA3, JBA6, and characterized one representative JBA6 endolysin, PlyJBA6. Transmission electron microscopy and genomic analysis demonstrated that all three phages belong to the Salasmaviridae family, characterized by short, non-contractile tails with linear dsDNA genomes ranging from 18.7 to 19.1 kb. PlyJBA6 contains a glycoside hydrolase family 24 domain (PF00959) at the N-terminus and two LysM domains (PF04176) at the C-terminus. While JBA6 has a narrow host range, infecting only 7 out of 9 tested strains of B. amyloliquefaciens, PlyJBA6 exhibits extended lytic range beyond the Bacillus genus. Interestingly, PlyJBA6 lyses Gram-negative bacteria such as Escherichia coli, Yersinia enterocolitica and Cronobacter sakazakii without other additives to destabilize bacterial outer membrane. We assume that JBA6 might be a useful component for a phage cocktail to control B. amyloliquefaciens and that PlyJBA6 can provide insights into the development of novel biocontrol agents against various food-borne pathogens.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502026"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11985411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aspirin Combined with Antifungal Drugs Suppresses Candida albicans Biofilm by Activating Autophagy. 阿司匹林与抗真菌药物联用可通过激活自噬抑制白色念珠菌生物膜

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-27 DOI: 10.4014/jmb.2411.11060

Yun Huang, Haochen Miao, Ying Lv, Yang Wang, Shenjun Yu, Xin Wei

{"title":"Aspirin Combined with Antifungal Drugs Suppresses Candida albicans Biofilm by Activating Autophagy.","authors":"Yun Huang, Haochen Miao, Ying Lv, Yang Wang, Shenjun Yu, Xin Wei","doi":"10.4014/jmb.2411.11060","DOIUrl":"10.4014/jmb.2411.11060","url":null,"abstract":"Aspirin (ASA) induces autophagic death of human tumor cells and autophagy changes the susceptibility of Candida albicans biofilm to antifungal agents. This study investigates whether ASA suppresses C. albicans biofilm by autophagy regulation and its combination effect with antifungals. Biofilm sensitivity to ASA alone and in combination with antifungals was evaluated using the checkerboard method, and drug interactions were assessed by the fractional inhibition concentration index (FICI) and ΔE models. The effects of ASA on mTOR signaling were examined by western blotting. Alkaline phosphatase activity, acridine orange stain assay, and autophagy-related gene expressions were examined to evaluate autophagic activity. Autophagosomes were observed by transmission electron microscopy. Reactive oxygen species (ROS) were detected by DCFH-DA. Mitochondrial membrane potential (MMP), malondialdehyde (MDA), and ATP levels were determined using commercial kits. ASA inhibited C. albicans biofilm in a concentration dependent manner and showed synergistic effects against biofilms when combined with amphotericin B or 5-fluorocytosine. ASA treatment induced oxidative stress, evidenced by increased ROS and MDA levels, alongside a reduction in ATP and MMP. ASA inhibited mTOR signaling and induced autophagy in C. albicans biofilms by increasing oxidative stress and mitochondrial dysfunction, contributing to biofilm inhibition. This study provides valuable insights into the potential of ASA as an adjunct therapy in combination with antifungal agents for managing C. albicans biofilm-related infections.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411060"},"PeriodicalIF":2.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11985414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Therapeutic Potential of Cordyceps Mushroom on SARS-CoV-2 Using Virtual Screening against M^pro and In Vitro Validation of Cordycepin. 利用针对 Mpro 的虚拟筛选和虫草素的体外验证，探索冬虫夏草蘑菇对 SARS-CoV-2 的治疗潜力。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-26 DOI: 10.4014/jmb.2411.11063

Mohammad Hassan Baig, Ayman Turk, Preeti Vishwakarma, Yun Seong Jo, Jae-June Dong, Dae Hee Lee, Young Guk Kim, Mi Kyeong Lee, Jae-Yong Cho

{"title":"Exploring the Therapeutic Potential of Cordyceps Mushroom on SARS-CoV-2 Using Virtual Screening against Mpro and In Vitro Validation of Cordycepin.","authors":"Mohammad Hassan Baig, Ayman Turk, Preeti Vishwakarma, Yun Seong Jo, Jae-June Dong, Dae Hee Lee, Young Guk Kim, Mi Kyeong Lee, Jae-Yong Cho","doi":"10.4014/jmb.2411.11063","DOIUrl":"10.4014/jmb.2411.11063","url":null,"abstract":"Pathogenic coronavirus, including COVID-19, threatens human health, and there have been strong demands for efficient therapeutics. Cordyceps militaris is a medicinal mushroom that has long been used for immune enhancement, anticancer, and antiviral effects. Therefore, the inhibitory potentials of constituents of C. militaris against COVID-19 were analyzed using various virtual screening analyses. Among ten constituents of C. militaris, cordycepin, the major component, and 3'-deoxyuridine and 2'-O-methyl-adenosine showed strong binding affinity to Mpro, a potential target for COVID-19 therapeutics. Considering the structure-activity relationship, nucleosides having deoxyribose and methoxyribose moiety are important for the affinity to Mpro. Cordycepin is also bound to Mpro mutants, and the binding mechanisms between cordycepin and Mpro were investigated further by MD simulation and MM/PBSA analysis. Principal component analysis also confirmed the conformational change of Mpro by cordycepin, which inhibits the function of Mpro. In vitro, the efficacy of cordycepin was measured using Vero cells infected with SARS-CoV-2, which showed excellent inhibition with an IC50 value of 29 μM. Conclusively, the constituents of C. militaris are expected to inhibit SARS-CoV-2 replication through binding to Mpro. Therefore, C. militaris can be an essential therapeutic for coronavirus through the synergistic effect of its constituents.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411063"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11985406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0