Journal of microbiology and biotechnology最新文献_第8页

Therapeutic Potential of Lactobacillus rhamnosus DS3316 via Cell Apoptosis in Colorectal Cancer. 鼠李糖乳杆菌DS3316通过细胞凋亡治疗结直肠癌的潜力。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2505.05001

Jinkwon Lee, Jeongmin Lee, In Hwan Tae, Yunsang Kang, Jinsan Kim, Sarang Kim, Haneol Yang, Kunhyang Park, Doo-Sang Park, Dae-Soo Kim, Hyun-Soo Cho

{"title":"Therapeutic Potential of Lactobacillus rhamnosus DS3316 via Cell Apoptosis in Colorectal Cancer.","authors":"Jinkwon Lee, Jeongmin Lee, In Hwan Tae, Yunsang Kang, Jinsan Kim, Sarang Kim, Haneol Yang, Kunhyang Park, Doo-Sang Park, Dae-Soo Kim, Hyun-Soo Cho","doi":"10.4014/jmb.2505.05001","DOIUrl":"10.4014/jmb.2505.05001","url":null,"abstract":"Colorectal cancer (CRC) has a very high mortality rate worldwide. Although various therapies have been developed to treat CRC, the need for novel therapeutic approaches has been increasing due to severe side effects and limited efficacy of current treatments. Recently, although research on the gut microbiome and its association with colon cancer has been growing, the mechanisms of gut microbiome inhibition in CRC remain insufficiently understood. Thus, in this study, we investigated the growth-inhibitory effects of the culture supernatant of Lactobacillus rhamnosus DS3316, isolated from infant feces, on CRC cell lines (HCT116 and SNUC5). And RNA-seq analysis revealed an increase in apoptosis-related terms induced by L. rhamnosus DS3316 treatment. Also, we found the non-toxicity of L. rhamnosus DS3316 in human iPSC-derived intenstine organoid. Thus, we suggested that L. rhamnosus DS3316 inhibits the growth of colorectal cancer cell lines without affecting normal cells. And L. rhamnosus DS3316 is expected to be a promising candidate for the development of microbiome-based colorectal cancer therapies. Furthermore, its combined use with various colorectal cancer treatment methods could lead to the proposal of more effective therapeutic approaches.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505001"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomics-Metabolomics Reveals the Alleviation of Indole-3-Ethanol on Radiation-Induced Enteritis in Mice. 宏基因组学-代谢组学揭示吲哚-3-乙醇对小鼠放射性肠炎的缓解作用。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2502.02037

Hua Zhong, Yipeng Song, Shanliang Hu, Chengxu Zhang, Lili Li

{"title":"Metagenomics-Metabolomics Reveals the Alleviation of Indole-3-Ethanol on Radiation-Induced Enteritis in Mice.","authors":"Hua Zhong, Yipeng Song, Shanliang Hu, Chengxu Zhang, Lili Li","doi":"10.4014/jmb.2502.02037","DOIUrl":"10.4014/jmb.2502.02037","url":null,"abstract":"Indole-3-ethanol (IEt), a small molecule metabolite from intestinal microbial tryptophan metabolism, has been established to have anti-inflammatory properties. However, its effect on radiation-induced enteritis has not been reported. Here, we aim to explore the effects and potential mechanisms of IEt on radiation enteritis. C57BL/6J mice were orally administered an IEt solution before radiation exposure. Inflammatory factors, including IL-17A, IFN-γ, IL-6 and IL-1β, were detected using enzyme-linked immunosorbent assay. Colonic histopathology was assessed through H&E staining. Subsequently, gut microbiota and its metabolites were analyzed using metagenomics and metabolomics. The results suggested that IEt alleviated radiation-induced enteritis, as evidenced by improved colonic structural integrity, decreased levels of pro-inflammatory factors like IL-17A, and the restoration of intestinal microecological and metabolic balance. IEt enriched the abundance of Lachnospiraceae family members, particularly the genus Roseburia - a known anti-inflammatory commensal. In addition, IEt upregulated the levels of metabolites with anti-inflammatory effects such as indole-3-carbinol, pteridine, and pyropheophorbide-a. Furthermore, Roseburia was significantly positively correlated with indole-3-carbinol and negatively correlated with the pro-inflammatory factor IL-17A. Therefore, IEt may alleviate radiation enteritis through Roseburia-indole-3-carbinol and Roseburia-IL-17A axes. This study revealed the potential mechanisms by which IEt alleviated radiation enteritis, providing a potential protective candidate for radiation enteritis.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502037"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethyl Acetate Fraction from Eucommia ulmoides Ameliorates Particulate Matter (PM)_2.5-Induced Intestinal Damage by Restoring Barrier Integrity and Regulating Inflammatory Responses. 杜仲乙酸乙酯组分通过恢复屏障完整性和调节炎症反应改善颗粒物（PM 2.5）诱导的肠道损伤

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2504.04002

Min Ji Kim, Jong Min Kim, Hyo Lim Lee, Ho Jin Heo

{"title":"Ethyl Acetate Fraction from Eucommia ulmoides Ameliorates Particulate Matter (PM)2.5-Induced Intestinal Damage by Restoring Barrier Integrity and Regulating Inflammatory Responses.","authors":"Min Ji Kim, Jong Min Kim, Hyo Lim Lee, Ho Jin Heo","doi":"10.4014/jmb.2504.04002","DOIUrl":"10.4014/jmb.2504.04002","url":null,"abstract":"This study investigated the protective effect of ethyl acetate fraction from Eucommia ulmoides leaf (EFEL) against intestinal dysfunction induced by chronic exposure to particulate matter (PM)2.5 in BALB/c mice. EFEL treatment suppressed reactive oxygen species (ROS) production and cellular death from PM2.5-induced HT29 cells. EFEL supplementation ameliorated PM2.5-induced intestinal damage by regulating antioxidant biomarkers, including reduced glutathione and superoxide dismutase, malondialdehyde levels, and myeloperoxidase activity. EFEL modulated the gut microbiota composition by increasing beneficial bacteria, such as Lactobacillus and Alistipes, and reducing the abundance of pathogenic bacteria, such as Helicobacter and Clostridia UCG-014, thus contributing to the restoration of the intestinal microenvironment. Furthermore, EFEL regulated the expression of tight junction proteins and inflammatory biomarkers in intestinal tissue. These findings suggest that EFEL may serve as a promising functional food material with the potential to alleviate PM2.5-induced intestinal barrier dysfunction by regulating oxidative stress, inflammation, and microbiota homeostasis.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504002"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Conserved Genetic Factors Regulating bla_NDM Gene Expression. 调控blaNDM基因表达的高度保守遗传因子。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-14 DOI: 10.4014/jmb.2412.12081

Jianfen Xu, Jinnuo Zhu, Changqing Mei, Xiaopeng Liu, Zhiming Gong, Jiansheng Huang, Hui Chai

{"title":"Highly Conserved Genetic Factors Regulating blaNDM Gene Expression.","authors":"Jianfen Xu, Jinnuo Zhu, Changqing Mei, Xiaopeng Liu, Zhiming Gong, Jiansheng Huang, Hui Chai","doi":"10.4014/jmb.2412.12081","DOIUrl":"10.4014/jmb.2412.12081","url":null,"abstract":"The New Delhi metallo-β-lactamase (NDM; EC 3.5.2.6) poses significant challenges to carbapenem treatment, yet the regulatory mechanisms governing blaNDM gene expression remain poorly understood. In this study, we identified a highly conserved 110 bp sequence located upstream of the blaNDM gene by comparative analysis of 109 clinical isolates and 2,706 nucleotide sequences from the NCBI database. This conserved sequence was characterized in all available NDM variants spanning 80 bacterial species. Bioinformatics analysis revealed a core promoter (PNDM) and two transcription factor binding sites (ArcA and ArgR2) within the sequence. Mutations of the PNDM promoter significantly reduced blaNDM expression (mRNA and protein) by more than 90% (P < 0.01) and completely restored carbapenem susceptibility. Electrophoretic mobility shift assays (EMSA) confirmed the specific binding of ArcA and ArgR2 proteins to their predicted sites. Mutations in the ArcA and ArgR2 binding sites decreased blaNDM protein production with less pronounced effects compared to PNDM promoter mutations, resulting in 24% and 32.7% reductions, respectively. The findings highlight the critical role of the highly conserved 110 bp sequence in regulating blaNDM expression, offering potential targets for novel therapeutic strategies.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412081"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yeast Prions: Discovery, Nature, Cellular Manipulation and Implication. 酵母朊病毒：发现，性质，细胞操作和启示。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-14 DOI: 10.4014/jmb.2503.03046

Moonil Son

{"title":"Yeast Prions: Discovery, Nature, Cellular Manipulation and Implication.","authors":"Moonil Son","doi":"10.4014/jmb.2503.03046","DOIUrl":"10.4014/jmb.2503.03046","url":null,"abstract":"Prion is simply an infectious protein, and it occurs spontaneously without any well-defined reason. Once prions occurred, they mostly propagated in biophysically very stable amyloid form with self-templating mechanism. Human and mammalian prion results in untreatable and even fatal brain disorders, thus study of prion and its pathology extremely difficult. However, since the first discovery of two prions in model microorganism Saccharomyces cerevisiae, studies about prion biology, host physiology affected by prion or prion disease have facilitated. Moreover, many attempts to understand human and mammalian prion diseases were applicated based on what have done from yeast-prion system. In this review, progressive advances, from early experiment recognitions about prion even before actual proof to current advances in prion research, will be discussed, and from the fundamentals, such as yeast prion manipulation, prion biology including prion domain and transmission, to in-depth achievements of prion amyloid structure and anti-prion systems will be presented. Lastly, the impact of yeast prion study on other kingdom such as bacteria and human biomedical research, and recent development of basic cellular physiology.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503046"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of Ginseng Soluble Dietary Fiber and Its Fecal Microbiota Extract on Antibiotic-Induced Gut Dysbiosis Obese Mice. 人参可溶性膳食纤维及其粪便微生物群提取物对抗生素诱导的肥胖小鼠肠道生态失调的保护作用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-14 DOI: 10.4014/jmb.2502.02013

Luran Yang, Mei Hua, Da Li, Fan Li, Yuguang He, Xinyu Miao, Mubai Sun, Honghong Niu, Fenghao An, Jing Wang, Min Yang, Jinyuan Lu, Hongyan Xu, Jinghui Wang

{"title":"Protective Effects of Ginseng Soluble Dietary Fiber and Its Fecal Microbiota Extract on Antibiotic-Induced Gut Dysbiosis Obese Mice.","authors":"Luran Yang, Mei Hua, Da Li, Fan Li, Yuguang He, Xinyu Miao, Mubai Sun, Honghong Niu, Fenghao An, Jing Wang, Min Yang, Jinyuan Lu, Hongyan Xu, Jinghui Wang","doi":"10.4014/jmb.2502.02013","DOIUrl":"10.4014/jmb.2502.02013","url":null,"abstract":"Prolonged or improper antibiotic use may increase the risk of obesity. Ginseng soluble dietary fiber (G-SDF) has been shown to inhibit obesity and promote the growth of intestinal probiotics. However, its role in antibiotic-induced gut dysbiosis obese mice (ADIO) remains unclear, and this study aimed to elucidate this role. The results indicated that G-SDF and its fecal microbiota extract (SDFfbs) significantly reduced body weight, insulin resistance, hepatic fat accumulation, abnormal blood and liver glucose-lipid metabolism, oxidative stress, and immune-inflammatory responses in ADIO mice. G-SDF and SDFfbs also inhibited the LPS/TLR4/MyD88/NF-κB signaling pathway, restored the expression of the gut barrier proteins Occludin and Claudin1, and protected against intestinal damage in ADIO mice. In particular, G-SDF and SDFfbs significantly increased the abundance of Firmicutes and Bacteroidetes and decreased the abundance of harmful Escherichia and Streptococcus. Additionally, they promoted the growth of beneficial bacteria, such as Enterococcus, Lactobacillus, Bifidobacterium, Parabacteroides, and Akkermansia, and these microbial shifts correlated with significant improvements in metabolic indicators in ADIO mice. Notably, SDFfbs can replicate the efficacy of SDF and has even shown stronger effects than the latter. In summary, this study demonstrated that G-SDF and SDFfbs effectively mitigate the double damage caused by obesity and antibiotic exposure by modulating the LPS/TLR4/MyD88/NF-κB pathway, protecting the intestinal barrier, and restoring the gut microbiota balance. These findings provide an important theoretical basis for the use of G-SDF and SDFfbs as fat-reducing and antibiotic-resistant ingredients in health foods.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502013"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Skin-Protective Effects of a Novel Centella asiatica Variety (BT-Care) Cultivated for 75 Days via Modulation of Antioxidant Defense, Collagen Synthesis, and Skin Barrier Function. 通过调节抗氧化防御、胶原合成和皮肤屏障功能，培育75天的积雪草新品种（BT-Care）的皮肤保护作用增强。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-14 DOI: 10.4014/jmb.2504.04036

Kayoung Ko, Ga Yeong Cheon, Yeon Ji Ha, Ye Rin Ko, Jeong Hwan Kim, Daebang Seo, Sook Young Park, Bongkyu Lee, Ki-Bae Hong

{"title":"Enhanced Skin-Protective Effects of a Novel Centella asiatica Variety (BT-Care) Cultivated for 75 Days via Modulation of Antioxidant Defense, Collagen Synthesis, and Skin Barrier Function.","authors":"Kayoung Ko, Ga Yeong Cheon, Yeon Ji Ha, Ye Rin Ko, Jeong Hwan Kim, Daebang Seo, Sook Young Park, Bongkyu Lee, Ki-Bae Hong","doi":"10.4014/jmb.2504.04036","DOIUrl":"10.4014/jmb.2504.04036","url":null,"abstract":"Centella asiatica L. Urban (CA) is traditionally used in skin wound healing, and stable cultivation-related studies are currently being conducted to meet the growing demand in the food, cosmetics, and pharmaceutical industries. This study aimed to analyze the growth rate and target bioactive compound levels of a novel variety, giant CA (BT-care), according to cultivation period, and to compare the effects of extracts harvested at specific cultivation periods on skin health in human keratinocytes with those of general CA. Although yields increased with prolonged BT-care cultivation, the levels of madecassoside, asiaticoside, total polyphenols and radical scavenging activity were significantly higher at 75 days post-transplantation. Compared to general CA, BT-care extracts obtained at day 75 significantly enhanced glutathione peroxidase expression among antioxidant-associated genes in ultraviolet B (UVB)-irradiated HaCaT cells. In addition, BT-care dose-dependently upregulated gene expression involved in collagen synthesis, wrinkle prevention, and skin barrier reinforcement. Furthermore, the high-concentration of BT-care significantly reduced the phosphorylation of c-JUN NH2-terminal protein kinase and p38 mitogen-activated protein kinases. After 75 days of cultivation, BT-care significantly modulated protein levels associated with skin homeostasis, recovery, collagen synthesis, and skin barrier-related gene expression, which may be attributed to the elevated levels of active compounds. These findings indicate that BT-care may serve as a promising resource for food, cosmetic, and pharmaceutical applications aimed at promoting skin health.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504036"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and Evaluation of the Biological Activity of Recombinant Mouse Prolactin. 重组小鼠催乳素的纯化及生物活性评价。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-14 DOI: 10.4014/jmb.2503.03029

Yoon-A Shin, Holim Jin, Yongjin Lee, Han-Byeol Shin, Young-Jin Son

{"title":"Purification and Evaluation of the Biological Activity of Recombinant Mouse Prolactin.","authors":"Yoon-A Shin, Holim Jin, Yongjin Lee, Han-Byeol Shin, Young-Jin Son","doi":"10.4014/jmb.2503.03029","DOIUrl":"10.4014/jmb.2503.03029","url":null,"abstract":"Prolactin, a hormone secreted by lactotroph cells in the anterior pituitary lobe, plays a crucial role in vascular growth and immune homeostasis. In this study, we evaluated the functional activity of recombinant mouse prolactin produced using Escherichia coli. By employing a maltose-binding protein tag and TEV protease cleavage, we enhanced protein solubility and stability while ensuring high bioactivity. The recombinant prolactin was purified to over 99% purity through a streamlined two-step chromatography process, as confirmed by SDS-PAGE and Western blot analyses. Cell-based assays verified that the purified prolactin retained its biological activity, indirectly demonstrating its structural and functional stability. Additionally, the cost-effectiveness and simplicity of this method were highlighted by the reusability of the affinity column, reducing purification costs compared to conventional methods. This study provides a simple purification method for obtaining activated recombinant mouse prolactin, suggesting its potential use in biological and biochemical research.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503029"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Activity of Bacteriocin-Like Inhibitory Substances (BLIS) of Levilactobacillus brevis PG-11 against Salmonella Typhimurium. 短乳杆菌PG-11细菌素样抑制物质（BLIS）对鼠伤寒沙门菌的抑菌活性研究

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-14 DOI: 10.4014/jmb.2502.02011

Guo Pan, Xinling Zhao, Changjiang Wang, Yezhengzheng Tian, Jingyu Gao, Wanli Sha, Baishuang Yin, Wenlong Dong

{"title":"Antibacterial Activity of Bacteriocin-Like Inhibitory Substances (BLIS) of Levilactobacillus brevis PG-11 against Salmonella Typhimurium.","authors":"Guo Pan, Xinling Zhao, Changjiang Wang, Yezhengzheng Tian, Jingyu Gao, Wanli Sha, Baishuang Yin, Wenlong Dong","doi":"10.4014/jmb.2502.02011","DOIUrl":"10.4014/jmb.2502.02011","url":null,"abstract":"Salmonella Typhimurium is a major cause of foodborne illness. This study investigated the antibacterial activity, characterization, and mode of action of bacteriocin-like inhibitory substances (BLIS) produced by Levilactobacillus brevis PG-11, isolated from traditional Chinese kimchi. The BLIS of L. brevis PG-11 exhibited strong antibacterial activity against S. Typhimurium ATCC 14028. It also exhibited broad-spectrum antimicrobial activity against various foodborne and human pathogens, including Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus epidermidis. The minimum inhibitory concentration (MIC) of the BLIS against E. coli, S. Typhimurium, and S. aureus was 62.5, 125, and 125 mg/ml. The active antibacterial substances were partially purified using dialysis and tricine-SDS-PAGE, revealing a molecular weight of less than 1 kDa for the active antibacterial substances. Furthermore, the BLIS was stable under acidic pH (pH 3.0-6.0), heat treatment (121°C, 20 min), UV radiation (4 h), and sensitivity to papain. Maximum antibacterial activity was observed during the stationary growth phase after 32 h of culture. Propidium iodide (PI) uptake, ATP leakage, and scanning electron microscopy (SEM) confirmed that BLIS disrupted cell membrane integrity, leading to cell membrane damage pore formation, and bacterial growth inhibition. These results highlight the potential of L. brevis PG-11 BLIS as a natural biological preservative for food preservation applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502011"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Screening of Plastic-Degrading Enzymes Using an Optimized Cell-Free Protein Synthesis Platform. 利用优化的无细胞蛋白合成平台快速筛选塑料降解酶。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-14 DOI: 10.4014/jmb.2503.03044

SangKu Yi, Junhyeon Park, Jiyoung Park, Kyung-Jin Kim, Juhyun Kim

{"title":"Rapid Screening of Plastic-Degrading Enzymes Using an Optimized Cell-Free Protein Synthesis Platform.","authors":"SangKu Yi, Junhyeon Park, Jiyoung Park, Kyung-Jin Kim, Juhyun Kim","doi":"10.4014/jmb.2503.03044","DOIUrl":"10.4014/jmb.2503.03044","url":null,"abstract":"The accumulation of plastic waste poses a significant environmental challenge, necessitating the development of efficient plastic-degrading enzymes for bioremediation and recycling. However, traditional enzyme engineering approaches rely on microbial expression systems and are time-consuming and prone to unintended interactions between host cells and recombinant circuits. To address these limitations, a cell-free protein synthesis (CFPS) platform was developed for rapidly screening plastic-degrading enzymes. Using CFPS, cutinase and PET-degrading enzymes (PETases) were successfully synthesized, and their catalytic activities were assessed using polymer-containing agar plates. Clear degradation halos were observed for cutinase and PETase on polycaprolactone (PCL)-containing and bis (2-hydroxyethyl) terephthalate (BHET)-containing plates, respectively. The optimization of CFPS conditions revealed that enzyme synthesis efficacy was higher at room temperature than at 37°C. The screening of PETase variants (C3 N1377, Mipa-P, and C5 N1251), synthesized using the CFPS platform, demonstrated that the catalytic activity of Mipa-P was the highest and surpassed that of IsPETase. This finding was further validated through purified enzyme analysis. Our results establish CFPS as a rapid, scalable, and cell-free alternative platform for screening and optimizing plastic-degrading enzymes, facilitating advancements in enzymatic plastic recycling.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503044"},"PeriodicalIF":2.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0