Journal of microbiology and biotechnology最新文献_第7页

A Strictly Inducible and Orthogonal Dre-rox System for Precise and Markerless Genome Editing in Bacillus subtilis. 枯草芽孢杆菌精确无标记基因组编辑的严格诱导正交re-rox系统

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-25 DOI: 10.4014/jmb.2505.05006

Jianan Lv, Gang Fu, Qiyao Zhu, Wenhui You, Fengming Guo, Rong Li, Dawei Zhang

{"title":"A Strictly Inducible and Orthogonal Dre-rox System for Precise and Markerless Genome Editing in Bacillus subtilis.","authors":"Jianan Lv, Gang Fu, Qiyao Zhu, Wenhui You, Fengming Guo, Rong Li, Dawei Zhang","doi":"10.4014/jmb.2505.05006","DOIUrl":"10.4014/jmb.2505.05006","url":null,"abstract":"Site-specific recombination enables precise and modular genome engineering in microbial systems. In Bacillus subtilis, Cre is the most commonly used site-specific recombinase (SSR) and has been widely applied in genome engineering. Developing SSRs with comparable performance to Cre that can also function orthogonally would significantly expand the genome engineering toolkit. We established a resistance gene-based reporter in B. subtilis to assess the genome editing potential of the Dre-rox system. A theophylline-inducible riboswitch tightly controlled Dre expression to minimize leaky recombination, improving the specificity of rox-mediated recombination. Notably, Dre and Cre function without crosstalk at their respective recognition sites. This orthogonal combination enabled a modular workflow: Cre-mediated integration followed by Dre-mediated markerless deletion. Dual and triple-site models confirmed that Dre-rox supports synchronized multi-locus excision with a single induction. Optimized Dre-rox architecture highlighted its reliability for genome engineering in B. subtilis. The system features high-fidelity recombination, low toxicity, and strong host adaptability. This work extends Dre-rox utility to prokaryotic systems. The standardized Dre-rox platform provides a foundation for hierarchical pathway engineering, mutant library generation, and modular chassis development in synthetic biology.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505006"},"PeriodicalIF":3.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12331466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and Tolerability of HemoHIM: A Randomized, Placebo-Controlled, Double-Blind, and Parallel Clinical Trial in Healthy Human Volunteers. 血液him的安全性和耐受性：一项随机、安慰剂对照、双盲、平行的健康人类志愿者临床试验。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-25 DOI: 10.4014/jmb.2503.03041

Ji-Won Seo, Jun-Hyun Bae, Ju Gyeong Kim, Su-Bin Bak, Gyoung-Deuck Kim, Wook Song

{"title":"Safety and Tolerability of HemoHIM: A Randomized, Placebo-Controlled, Double-Blind, and Parallel Clinical Trial in Healthy Human Volunteers.","authors":"Ji-Won Seo, Jun-Hyun Bae, Ju Gyeong Kim, Su-Bin Bak, Gyoung-Deuck Kim, Wook Song","doi":"10.4014/jmb.2503.03041","DOIUrl":"10.4014/jmb.2503.03041","url":null,"abstract":"HemoHIM is a mixed extract derived from the roots of oriental medicinal herbs. Despite many studies on HemoHIM's antioxidant properties and its effect on immune functions, fatigue reduction, and exercise performance, published data regarding its physiological safety in humans are lacking. Therefore, this study aimed to investigate the safety profile of HemoHIM in humans. The samples were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with ultra-high-performance LC coupled to an Orbitrap MS. Data processing and compound identification were performed through nontargeted screening using multiple spectral databases. Ninety-six healthy adults were recruited in the clinical study. On visit 1, screening was conducted to assess eligibility. On visit 2, participants were randomly assigned to either the HemoHIM group (n = 48) or the placebo group (n = 48), and they consumed 40 g/day either HemoHIM or placebo supplements twice daily for 8 week. Participants visited the laboratory four times for body composition, compliance, dietary intake, vital signs, blood and urine biomarker, and adverse event assessment. Several bioactive compounds were successfully identified using LC-heated electrospray ionization-Orbitrap-MS/MS, including chlorogenic acid, paeoniflorin, decursin, and nodakenin. No clinically significant changes were found in the safety test of vital signs, blood and urine biomarkers, and adverse events. Based on these findings, HemoHIM could be a safe and beneficial natural compound mixture for human use.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503041"},"PeriodicalIF":3.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dendranthema boreale (Makino) Ling ex Kitam. Flower Extract Ameliorates Oxidative Stress-Induced Cellular Damage in HaCaT Keratinocytes by Regulating MAPK Signaling. 白菊（牧野）林，北野。花提取物通过调节MAPK信号通路改善氧化应激诱导的HaCaT角质形成细胞损伤。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2505.05005

You Kyeong Lee, Parkyong Song, Seo Young Choi, Mi Song Shin, Ji Sun Hwang, Hong-Joo Son, Yu-Jin Kim, Wanil Kim, Kwang Min Lee

{"title":"Dendranthema boreale (Makino) Ling ex Kitam. Flower Extract Ameliorates Oxidative Stress-Induced Cellular Damage in HaCaT Keratinocytes by Regulating MAPK Signaling.","authors":"You Kyeong Lee, Parkyong Song, Seo Young Choi, Mi Song Shin, Ji Sun Hwang, Hong-Joo Son, Yu-Jin Kim, Wanil Kim, Kwang Min Lee","doi":"10.4014/jmb.2505.05005","DOIUrl":"10.4014/jmb.2505.05005","url":null,"abstract":"Oxidative stress plays a critical role in skin aging and in various dermatological disorders by promoting inflammation, apoptosis, and cellular dysfunction. Among reactive oxygen species (ROS), hydrogen peroxide (H2O2) readily penetrates cell membranes, triggering oxidative damage. This study investigated the protective effects of the Dendranthema boreale (Makino) Ling ex Kitam. flower extract (DBE) against H2O2-induced oxidative stress in HaCaT keratinocytes and explored the underlying molecular mechanisms. DBE (30-80 μg/ml) significantly attenuated H2O2-induced cytotoxicity by reducing cleaved caspase-3 activation and lowering the Bax/Bcl-2 ratio, thereby inhibiting apoptosis. Furthermore, DBE selectively suppressed JNK and ERK phosphorylation while having no effect on p38 MAPK activation. Inflammatory responses were also modulated, as DBE inhibited NF-κB p65 phosphorylation and downregulated COX-2 expression, a key mediator of oxidative stress-induced inflammation. These findings indicate that DBE protects HaCaT keratinocytes from oxidative stress-induced cellular damage by promoting cell survival, suppressing apoptosis, and modulating the key signaling pathways involved in oxidative stress and inflammation. This study provides foundational insights into the potential therapeutic and cosmetic applications of DBE for the prevention of oxidative stress-related skin disorders.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505005"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12331467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1,2,3,6-Tetra-O-Galloyl-β-D-Glucopyranose Induces Apoptosis and Ferroptosis in Colon Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway. 1,2,3,6- 4 - o -没食子酰-β- d -葡萄糖吡喃糖通过抑制Wnt/β-Catenin信号通路诱导结肠癌细胞凋亡和铁下垂

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2503.03050

Suhyeon Kim, MinKyun Na, Sangtaek Oh

{"title":"1,2,3,6-Tetra-O-Galloyl-β-D-Glucopyranose Induces Apoptosis and Ferroptosis in Colon Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.","authors":"Suhyeon Kim, MinKyun Na, Sangtaek Oh","doi":"10.4014/jmb.2503.03050","DOIUrl":"10.4014/jmb.2503.03050","url":null,"abstract":"Molecular irregularities in the canonical Wnt pathway that lead to the stabilization of β-catenin are common in colon cancer. Here, we identified 1,2,3,6-Tetra-O-galloyl-β-D-glucopyranose (TAGP), separated from Trapa japonica, as an inhibitor of canonical Wnt signaling. TAGP facilitated the phosphorylation of Ser33/37 and the degradation of β-catenin, which had accumulated due to Wnt3a-conditioned medium or the inhibitor 6-bromoindirubin-3'-oxime that targets glycogen synthase kinase-3β (GSK-3β). Additionally, TAGP lowered the levels of Cyclin D1 and c-Myc, which are regulated by β-catenin/T-cell factor (TCF) and showed antiproliferative activity in colon cancer cells. Furthermore, TAGP triggered apoptosis, as demonstrated by the activation of caspases 3 and 7, in conjunction with raising the number of Annexin-V-positive cells. It also promoted ferroptosis, as shown by the buildup of lipid peroxides and Fe2+ in the cells. Taken together, TAGP enhances β-catenin turnover, indicating its potential as a chemotherapeutics for colon cancer in humans.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503050"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Epidemiology of Norovirus Outbreaks in Childcare Centers and Schools in South Korea in 2023. 2023年韩国托儿中心和学校诺如病毒爆发的分子流行病学研究

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2503.03025

Won-Jeong Park, Byeong Joon Kim, Doo Won Seo, Yong Chjun Park, Insun Joo, Soo Hwan Suh

{"title":"Molecular Epidemiology of Norovirus Outbreaks in Childcare Centers and Schools in South Korea in 2023.","authors":"Won-Jeong Park, Byeong Joon Kim, Doo Won Seo, Yong Chjun Park, Insun Joo, Soo Hwan Suh","doi":"10.4014/jmb.2503.03025","DOIUrl":"10.4014/jmb.2503.03025","url":null,"abstract":"Norovirus is a leading cause of acute gastroenteritis and foodborne illness worldwide. In this study, we investigated the epidemiologic and molecular characteristics of norovirus outbreaks in childcare centers and schools in South Korea throughout 2023. A total of 141 stool samples collected from these outbreaks were confirmed positive for norovirus using real-time and conventional RT-PCR, and subsequently analyzed for genotype. The reported outbreaks were most frequently observed in the provinces of Gyeonggi (31.2%) and South Gyeongsang (20.6%), followed by Seoul (12.1%). Outbreaks were most frequently associated with childcare centers (38.3%), primary schools (36.2%), and kindergartens (13.5%). Seasonally, 51.8% of cases occurred during the winter months (November-March), with a peak observed in April. Genotypic analysis revealed that 95.6% of cases were caused by GII norovirus, with the GII.2[P16] genotype being the most prevalent (34.5%). Notably, foodborne transmission was implicated in 13.5% of cases, predominantly involving the GII.2[P16] and GII.6 genotypes. Unlike previous studies that report norovirus genotypes from clinical cases of acute gastroenteritis, our analysis included cases from foodborne outbreaks, thereby offering deeper insights into the role of contaminated food in facilitating norovirus transmission. Furthermore, childcare centers were the primary setting for detection of the GII.4[P16] and GII.4[P31] genotypes, while primary schools exhibited the greatest genotypic diversity, with 12 distinct genotypes identified. These findings indicate a potential shift in norovirus seasonal patterns, with outbreaks extending into late spring. Overall, our results underscore the need for enhanced hygiene practices, robust surveillance systems, and targeted prevention strategies to mitigate norovirus transmission.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503025"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Divalent Cation Supplementation to Enhance the Production of the Kimchi Starter Strain Latilactobacillus curvatus WiKim0094. 优化二价阳离子添加以提高泡菜发酵剂弯乳杆菌产量

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2505.05011

Seulbi Kim, Chang Hee Jeong, Jong-Cheol Kim, Moeun Lee, Seul-Gi Jeong, Ho Myeong Kim, Hae Woong Park

{"title":"Optimizing Divalent Cation Supplementation to Enhance the Production of the Kimchi Starter Strain Latilactobacillus curvatus WiKim0094.","authors":"Seulbi Kim, Chang Hee Jeong, Jong-Cheol Kim, Moeun Lee, Seul-Gi Jeong, Ho Myeong Kim, Hae Woong Park","doi":"10.4014/jmb.2505.05011","DOIUrl":"10.4014/jmb.2505.05011","url":null,"abstract":"To support the industrial-scale production of kimchi starter cultures, this study aimed to enhance the cell density of Latilactobacillus curvatus WiKim0094 by optimizing the composition of divalent cations in a nutrient-limited medium. Screening experiments identified Mg2+, Mn2+, and Ca2+ as key factors that significantly promoted cell growth. Using a Box-Behnken design, their optimal concentrations were determined to be 3.0, 0.1, and 4.4 mM, respectively, resulting in a 5.7-fold increase in the maximum viable cell count compared with the control. Comparative transcriptomic analysis revealed that supplementation with these metal ions activated pathways associated with nucleic acid synthesis, energy metabolism, nutrient transport, cell structure formation, and stress resistance. These findings provide mechanistic insights into divalent cation-mediated enhancement of microbial growth and propose a cost-effective strategy for the large-scale cultivation of kimchi starter strains. Future studies will focus on validating this approach in large-scale bioreactors to facilitate industrial application.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505011"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Cell-Permeable Puromycin N-Acetyltransferase Confers Puromycin Resistance by Direct Protein Transduction. 重组细胞可透性嘌呤霉素n -乙酰转移酶通过直接蛋白转导赋予嘌呤霉素抗性。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2502.02049

Jiwon Choi, Kyung-Hee Cho, Jiwon Im, Yeeun Seo, Amitesh Sharma, Kartic, Shivani Devi, Nattan Stalin, Seo Jin Park, Tae-Sik Park

{"title":"Recombinant Cell-Permeable Puromycin N-Acetyltransferase Confers Puromycin Resistance by Direct Protein Transduction.","authors":"Jiwon Choi, Kyung-Hee Cho, Jiwon Im, Yeeun Seo, Amitesh Sharma, Kartic, Shivani Devi, Nattan Stalin, Seo Jin Park, Tae-Sik Park","doi":"10.4014/jmb.2502.02049","DOIUrl":"10.4014/jmb.2502.02049","url":null,"abstract":"Puromycin N-acetyltransferase (PAC) is an enzyme that catalyzes the acetylation of puromycin, an inhibitor of protein synthesis. The PAC gene is often co-transfected with genes of interest in the same vector to serve as a selective marker, conferring puromycin resistance to mammalian cells. Cell-penetrating peptides (CPPs), which are 5-30 amino acids in length, facilitate the translocation of functional cargoes across the cell membrane. Among these, the HIV-transactivator of transcription (TAT) sequence is widely applied for its cell-penetrating and protein-delivery capabilities. In this study, we investigated whether attachment of the TAT sequence to PAC (TAT-PAC) enables intracellular delivery of TAT-PAC protein into mammalian cells, thereby conferring puromycin resistance. A recombinant TAT-PAC protein was expressed in Escherichia coli and purified to homogeneity. The purified TAT-PAC protein retained enzymatic activity, with a specific activity of 197 nmol/min/mg. Intracellular delivery of TAT-PAC was confirmed using confocal microscopy and flow cytometry, employing an RFP (red fluorescent protein)-tagged TAT-PAC fusion protein. Treatment of HEK293 and SY5Y cells with TAT-PAC resulted in increased cell viability in the presence of puromycin, demonstrating its functionality as a selection marker. This study suggests the potential application of cell-permeable PAC protein for selection of co-delivered therapeutic or gene-editing proteins in mammalian cells, providing a promising alternative to traditional genetic selection methods.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502049"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12325001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Particulate Matter in Atopic Dermatitis through HDACs and Filaggrin Alteration. 颗粒物质通过HDACs和聚丝蛋白改变对特应性皮炎的影响。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2502.02047

Yoon Jin Roh, Kui Young Park, Na Yeon Koo, Yong Hee Choi, Hye Won Song, Sin Woo Sung, Ka Ram Kim, Seong Jun Seo, Joon Seok, Mi-Kyung Lee

{"title":"Effect of Particulate Matter in Atopic Dermatitis through HDACs and Filaggrin Alteration.","authors":"Yoon Jin Roh, Kui Young Park, Na Yeon Koo, Yong Hee Choi, Hye Won Song, Sin Woo Sung, Ka Ram Kim, Seong Jun Seo, Joon Seok, Mi-Kyung Lee","doi":"10.4014/jmb.2502.02047","DOIUrl":"10.4014/jmb.2502.02047","url":null,"abstract":"Atopic dermatitis (AD) is a skin condition that can be exacerbated by particulate matter (PM). The primary causes of AD are believed to be impairments in the skin barrier, such as filaggrin (FLG) abnormalities. Although there is substantial evidence for genetic factors contributing to AD, it is challenging to attribute the disease's predisposition solely to genetics. We hypothesize that PM may induce epigenetic modifications in AD, impacting FLG expression. Here, we assessed histone deacetylases (HDACs) and FLG levels under AD conditions with or without exposure to PM using qRT-PCR, western blotting, and immunofluorescence. Additionally, we observed changes in these molecules when co-treated with the HDAC inhibitor, trichostatin A (TSA). FLG levels tended to decrease when PM or IL-4/13 were given individually, and a further decrease upon IL-4/13 and PM cotreatment. Interestingly, HDAC3 and HDAC6 are increased when they were both given PM10 and IL-4/13 co-treatment compared to IL-4/13 treatment alone. FLG levels exhibited a significant restoration and the levels of HDACs were changed when treated with TSA than with IL-4/13 or IL-4/13+PM co-treatment. We found changes in FLG and HDACs in the AD-like in vivo model and in this model with PM. Our findings suggest that PM can induce epigenetic alterations in AD. Treatment with TSA ameliorates these effects on FLG expression, indicating its potential as a novel therapeutic approach for AD.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502047"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface and Functional Properties of Biosurfactant Produced by Bacillus rugosus HH2 Derived from Jeotgal. 牛膝菌衍生芽孢杆菌HH2生物表面活性剂的表面及功能特性研究

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2505.05029

Geum-Jae Jeong, Do-Kyun Kim, Kyung-Jin Cho, Eun-Jin Choi, Won-Kyo Jung, Fazlurrahman Khan, Young-Mog Kim

{"title":"Surface and Functional Properties of Biosurfactant Produced by Bacillus rugosus HH2 Derived from Jeotgal.","authors":"Geum-Jae Jeong, Do-Kyun Kim, Kyung-Jin Cho, Eun-Jin Choi, Won-Kyo Jung, Fazlurrahman Khan, Young-Mog Kim","doi":"10.4014/jmb.2505.05029","DOIUrl":"10.4014/jmb.2505.05029","url":null,"abstract":"Surfactants play a critical role in diverse industrial applications. However, concerns over the environmental persistence and toxicity of synthetic variants have prompted a shift toward sustainable biosurfactants. In this study, the surface properties of a biosurfactant produced by Bacillus rugosus HH2 isolated from Jeotgal, a traditional Korean fermented seafood, were investigated to evaluate its industrial applicability. The biosurfactant exhibited consistent and stable emulsifying performance across a wide range of salinity (3-18%), temperature (30-80°C), and pH (4-10) conditions. Emulsification assays with various hydrocarbon substrates revealed a performance equal to or better than that of the synthetic surfactant Triton X-100. While the biosurfactant generated a lower initial foam volume than Triton X-100, it maintained a significantly higher foam stability over 60 min, highlighting its suitability for applications that benefit from persistent and low-volume foaming. Optical microscopy showed that the biosurfactant produced smaller and more uniform bubbles than those of Triton X-100. Furthermore, the producing strain demonstrated strong hydrophobic interactions with hydrocarbons, such as hexadecane, toluene, and pyrene, supporting its potential utility in hydrocarbon-rich environments. Collectively, these findings highlight the multifunctional surface and functional activity and environmental robustness of the B. rugosus HH2-derived biosurfactant, indicating its potential as a sustainable and effective alternative for diverse industrial applications, including food processing, cosmetics, and environmental remediation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505029"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bufalin Suppresses Triple-Negative Breast Cancer Stem Cell Growth by Inhibiting the Wnt/β-Catenin Signaling Pathway. 蟾毒灵通过抑制Wnt/β-Catenin信号通路抑制三阴性乳腺癌干细胞生长

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-07-18 DOI: 10.4014/jmb.2503.03002

So Jin Park, Hye Jin Jung

{"title":"Bufalin Suppresses Triple-Negative Breast Cancer Stem Cell Growth by Inhibiting the Wnt/β-Catenin Signaling Pathway.","authors":"So Jin Park, Hye Jin Jung","doi":"10.4014/jmb.2503.03002","DOIUrl":"10.4014/jmb.2503.03002","url":null,"abstract":"Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with high mortality rates and limited targeted therapies. TNBC stem cells (TNBCSCs) contribute to tumor aggressiveness, metastasis, and treatment resistance. Targeting TNBCSCs represents a promising therapeutic strategy for improving patient outcomes. In this study, we investigated the inhibitory effects of bufadienolides-bufalin, bufotalin, and cinobufotalin-on TNBCSC growth. Among them, bufalin exhibited the strongest antiproliferative activity. We further examined bufalin's impact on TNBCSC self-renewal, cell cycle regulation, apoptosis, and the Wnt/β-catenin signaling pathway using in vitro and in vivo models. Bufalin effectively suppressed TNBCSC self-renewal in in vitro tumorsphere assays and significantly reduced tumor growth in an in vivo HCC1937 TNBCSC xenograft chorioallantoic membrane (CAM) model. Bufalin induced G0/G1 phase cell cycle arrest by downregulating key regulatory proteins, including c-myc, cyclin D1, and CDK4. It also promoted intrinsic apoptosis through nuclear fragmentation, mitochondrial membrane potential reduction, and caspase activation. Additionally, bufalin downregulated key CSC markers, such as CD133, CD44, ALDH1A1, Nanog, Oct4, and Sox2. Notably, bufalin suppressed the Wnt/β-catenin signaling pathway by reducing β-catenin mRNA and protein expression, leading to the downregulation of EGFR, a downstream target of Wnt signaling. Our findings highlight bufalin as a potent chemotherapeutic agent capable of inhibiting TNBCSC growth by targeting stemness, proliferation, and apoptosis through Wnt/β-catenin signaling suppression. These results provide a strong rationale for further investigation of bufalin as a potential therapeutic strategy for TNBC treatment.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503002"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0