Journal of microbiology and biotechnology最新文献_第7页

Effects of Metal and Metal Oxide Nanoparticles against Biofilm-Forming Bacteria: A Systematic Review. 金属和金属氧化物纳米粒子对生物膜形成细菌的影响：系统综述。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-15 DOI: 10.4014/jmb.2403.03029

Hend Algadi, Mohammed Abdelfatah Alhoot, Anis Rageh Al-Maleki, Neny Purwitasari

{"title":"Effects of Metal and Metal Oxide Nanoparticles against Biofilm-Forming Bacteria: A Systematic Review.","authors":"Hend Algadi, Mohammed Abdelfatah Alhoot, Anis Rageh Al-Maleki, Neny Purwitasari","doi":"10.4014/jmb.2403.03029","DOIUrl":"10.4014/jmb.2403.03029","url":null,"abstract":"Biofilm formation by bacteria poses a significant challenge across diverse industries, displaying resilience against conventional antimicrobial agents. Nanoparticles emerge as a promising alternative for addressing biofilm-related issues. This review aims to assess the efficacy of metal and metal oxide nanoparticles in inhibiting or disrupting biofilm formation by various bacterial species. It delineates trends, identifies gaps, and outlines avenues for future research, emphasizing best practices and optimal nanoparticles for biofilm prevention and eradication. Additionally, it underscores the potential of nanoparticles as substitutes for traditional antibiotics in healthcare and combating antibiotic resistance. A systematic literature search, encompassing Web of Science, PubMed, and Google Scholar from 2015 to 2023, yielded 48 publications meeting the review criteria. These studies employed diverse methods to explore the antibacterial activity of nanoparticles against biofilm-forming bacteria strains. The implications of this study are profound, offering prospects for novel antimicrobial agents targeting biofilm-forming bacteria, often resistant to conventional antibiotics. In conclusion, nanoparticles present a promising frontier in countering biofilm-forming bacteria. This review delivers a structured analysis of current research, providing insights into the potential and challenges of nanoparticle utilization against biofilm-related challenges. While nanoparticles exhibit inherent antimicrobial properties with applications spanning healthcare, agriculture, and industries, the review acknowledges limitations such as the narrow scope of tested nanoparticles and the imperative need for extensive research on long-term toxicity and environmental impacts.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1748-1756"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing Strain-Specific Simple Sequence Repeat (SSR) Markers for Chlorella sorokiniana. 为小球藻开发菌株特异性简单序列重复 (SSR) 标记。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-18 DOI: 10.4014/jmb.2404.04024

Mais Sweiss, Maen Hasan, Nidal Odat

{"title":"Developing Strain-Specific Simple Sequence Repeat (SSR) Markers for Chlorella sorokiniana.","authors":"Mais Sweiss, Maen Hasan, Nidal Odat","doi":"10.4014/jmb.2404.04024","DOIUrl":"10.4014/jmb.2404.04024","url":null,"abstract":"Chlorella sorokiniana green microalga offers many environmentally friendly applications, including wastewater treatment, biofertilizers, animal feed, and biofuel production. Different strains of C. sorokiniana have unique properties that may suit one application but not another. There is a need to distinguish between the many available strains of C. sorokiniana to choose the one that best fits the application. Consequently, our research goal was to develop strain-specific simple sequence repeat (SSR) markers to differentiate between the different strains. Seventeen markers spanning ten out of the twelve chromosomes of the C. sorokiniana genome were developed and validated on eight different strains from culture collections and our lab, and were then analyzed by fragment analysis. The results demonstrate the potential of these polymorphic markers to detect the genetic differences between the strains of C. sorokiniana, and to serve as useful tools for the intra-species population genetic analysis and conservation genetics studies of C. sorokiniana.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1848-1856"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of Recombinant Human Papillomavirus 16 L1 Protein for Incorporation with para-Azido-L-Phenylalanine. 重组人乳头瘤病毒 16 L1 蛋白与对位叠氮-L-苯丙氨酸的结合工程。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-08-09 DOI: 10.4014/jmb.2407.07033

Jinhyeon Kim, Ki Jun Jeong, Geun-Joong Kim, Jong-Il Choi

引用次数: 0

Kraft Lignin Decomposition by Forest Soil Bacterium Pseudomonas kribbensis CHA-19. 森林土壤假单胞菌 CHA-19 分解牛皮纸木质素。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-19 DOI: 10.4014/jmb.2406.06021

Dockyu Kim, Han-Woo Kim, Hyoungseok Lee

{"title":"Kraft Lignin Decomposition by Forest Soil Bacterium Pseudomonas kribbensis CHA-19.","authors":"Dockyu Kim, Han-Woo Kim, Hyoungseok Lee","doi":"10.4014/jmb.2406.06021","DOIUrl":"10.4014/jmb.2406.06021","url":null,"abstract":"Identification of the biochemical metabolic pathway for lignin decomposition and the responsible degradative enzymes is needed for the effective biotechnological valorization of lignin to renewable chemical products. In this study, we investigated the decomposition of kraft lignin by the soil bacterium Pseudomonas kribbensis CHA-19, a strain that can utilize kraft lignin and its main degradation metabolite, vanillic acid, as growth substrates. Gel permeation chromatography revealed that CHA-19 decomposed polymeric lignin and degraded dehydrodivanillin (a representative lignin model compound); however, the degradative enzyme(s) and mechanism were not identified. Quantitative polymerase chain reaction with mRNAs from CHA-19 cells induced in the presence of lignin showed that the putative genes coding for two laccase-like multicopper oxidases (LMCOs) and three dye-decolorizing peroxidases (DyPs) were upregulated by 2.0- to 7.9-fold compared with glucose-induced cells, which indicates possible cooperation with multiple enzymes for lignin decomposition. Computational homology analysis of the protein sequences of LMCOs and DyPs also predicted their roles in lignin decomposition. Based on the above data, CHA-19 appears to initiate oxidative lignin decomposition using multifunctional LMCOs and DyPs, producing smaller metabolites such as vanillic acid, which is further degraded via ortho- and meta-ring cleavage pathways. This study not only helps to better understand the role of bacteria in lignin decomposition and thus in terrestrial ecosystems, but also expands the biocatalytic toolbox with new bacterial cells and their degradative enzymes for lignin valorization.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1867-1875"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of Endophytic Fungi Isolated from Mangifera indica L. in Promoting Plant Growth. 从 Mangifera indica L. 分离的内生真菌在促进植物生长中的作用

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-19 DOI: 10.4014/jmb.2401.01034

Kanyapat Sonsiam, Orlavanh Sonesouphap, Anyaporn Sangkaew, Pariyanuj Chulaka, Prakitsin Sihanonth, Chulee Yompakdee

{"title":"Roles of Endophytic Fungi Isolated from Mangifera indica L. in Promoting Plant Growth.","authors":"Kanyapat Sonsiam, Orlavanh Sonesouphap, Anyaporn Sangkaew, Pariyanuj Chulaka, Prakitsin Sihanonth, Chulee Yompakdee","doi":"10.4014/jmb.2401.01034","DOIUrl":"10.4014/jmb.2401.01034","url":null,"abstract":"Endophytic fungi have been shown to synthesize bioactive secondary metabolites, some of which promote plant growth through various mechanisms. In our previous study, endophytic fungi were isolated from mango trees (Mangifera indica L.). The present study examined fifty endophytic fungal isolates for mineral solubilization activity, ammonia production, and siderophore production. It was shown that these isolates could produce phytohormones indole-3-acetic acid and gibberellic acid, as well as inhibit plant pathogens, specifically Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The results showed that all the isolated fungal endophytes exhibited various activities. Based on the findings, two fungal endophytes-Aureobasidium pullulans CY.OS 13 and Aspergillus tamarii CY.OS 144-were selected for dual inoculation in chili plants under pot-scale conditions to investigate their potential to improve growth-related traits such as seed germination, shoot and root length, biomass, and chlorophyll content. Seed treated with A. pullulans CY.OS 13 and/or A. tamarii CY.OS 144 showed a significant (p < 0.05) increase in seed germination and growth parameters of chili plants grown under pot-scale conditions. Particularly, chili plants whose seeds were injected with a combination of the two selected endophytic fungi showed the highest plant development traits. Therefore, the selected endophytic fungi have the potential to be used as biofertilizers, especially when combined. They could eventually replace chemical fertilizers because they are environmentally friendly, beneficial to humans, and can even promote sustainable agriculture.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1857-1866"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Optimization of Liquid Fermentation Medium and Antitumor Activity of the Mycelium on Phyllopora lonicerae. 液体发酵培养基的优化及菌丝体对忍冬疫霉的抗肿瘤活性研究

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-24 DOI: 10.4014/jmb.2405.05004

Min Liu, Lu Liu, Guoli Zhang, Guangyuan Wang, Ranran Hou, Yinghao Zhang, Xuemei Tian

{"title":"Study on Optimization of Liquid Fermentation Medium and Antitumor Activity of the Mycelium on Phyllopora lonicerae.","authors":"Min Liu, Lu Liu, Guoli Zhang, Guangyuan Wang, Ranran Hou, Yinghao Zhang, Xuemei Tian","doi":"10.4014/jmb.2405.05004","DOIUrl":"10.4014/jmb.2405.05004","url":null,"abstract":"Phylloporia lonicerae is an annual fungus that specifically parasitizes living Lonicera plants, offering significant potential for developing new resource food and medicine. However, wild resources and mycelium production of this fungus is limited, and its anti-tumor active ingredients and mechanisms remain unclear, hampering the development of this fungus. Thus, we optimized the fermentation medium of P. lonicerae and studied the anti-tumor activity of its mycelium. The results indicated that the optimum fermentation medium consisted of 2% sucrose, 0.2% peptone, 0.1% KH2PO4, 0.05% MgSO4·7H2O, 0.16% Lonicera japonica petals, 0.18% P fungal elicitor, and 0.21% L. japonica stem. The biomass reached 7.82 ± 0.41 g/l after 15 days of cultivation in the optimized medium, a 142% increase compared with the potato dextrose broth medium, with a 64% reduction in cultivation time. The intracellular alcohol extract had a higher inhibitory effect on A549 and Eca-109 cells than the intracellular water extract, with half-maximal inhibitory concentration values of 2.42 and 2.92 mg/ml, respectively. Graded extraction of the alcohol extract yielded petroleum ether phase, chloroform phase, ethyl acetate phase, and n-butanol phase. Among them, the petroleum ether phase exhibited a better effect than the positive control, with a half-maximal inhibitory concentration of 113.3 μg/ml. Flow cytometry analysis indicated that petroleum ether components could induce apoptosis of Eca-109 cells, suggesting that this extracted component can be utilized as an anticancer agent in functional foods. This study offers valuable technical support and a theoretical foundation for promoting the comprehensive development and efficient utilization of P. lonicerae.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1898-1911"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Characterization of an R-M System in Paracoccus denitrifican DYTN-1 to Improve the Plasmid Conjugation Transfer Efficiency. 鉴定和表征反硝化细菌 DYTN-1 中的 R-M 系统以提高质粒共轭转移效率

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-26 DOI: 10.4014/jmb.2402.02041

Yunpeng Shi, Wenyan Cao, Zhiping Zheng, Sha Xu, Lijuan Chai, Shenghu Zhou, Yu Deng

{"title":"Identification and Characterization of an R-M System in Paracoccus denitrifican DYTN-1 to Improve the Plasmid Conjugation Transfer Efficiency.","authors":"Yunpeng Shi, Wenyan Cao, Zhiping Zheng, Sha Xu, Lijuan Chai, Shenghu Zhou, Yu Deng","doi":"10.4014/jmb.2402.02041","DOIUrl":"10.4014/jmb.2402.02041","url":null,"abstract":"Paracoccus denitrificans has been identified as a representative strain with heterotrophic nitrification-aerobic denitrification capabilities (HN-AD), and demonstrates strong denitrification proficiency. Previously, we isolated the DYTN-1 strain from activated sludge, and it has showcased remarkable nitrogen removal abilities and genetic editability, which positions P. denitrificans DYTN-1 as a promising chassis cell for synthetic biology engineering, with versatile pollutant degradation capabilities. However, the strain's low stability in plasmid conjugation transfer efficiency (PCTE) hampers gene editing efficacy, and is attributed to its restriction modification system (R-M system). To overcome this limitation, we characterized the R-M system in P. denitrificans DYTN-1 and identified a DNA endonuclease and 13 DNA methylases, with the DNA endonuclease identified as HNH endonuclease. Subsequently, we developed a plasmid artificial modification approach to enhance conjugation transfer efficiency, which resulted in a remarkable 44-fold improvement in single colony production. This was accompanied by an increase in the frequency of positive colonies from 33.3% to 100%. Simultaneously, we cloned, expressed, and characterized the speculative HNH endonuclease capable of degrading unmethylated DNA at 30°C without specific cutting site preference. Notably, the impact of DNA methylase M9 modification on the plasmid was discovered, significantly impeding the cutting efficiency of the HNH endonuclease. This revelation unveils a novel R-M system in P. denitrificans and sheds light on protective mechanisms employed against exogenous DNA invasion. These findings pave the way for future engineering endeavors aimed at enhancing the DNA editability of P. denitrificans.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1826-1835"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Gut Microbial Lipid Metabolite 14(15)-EpETE Inhibits Substance P Release by Targeting GCG/PKA Signaling to Relieve Cisplatin-Induced Nausea and Vomiting in Rats. 肠道微生物脂质代谢物 14(15)- EpETE 通过靶向 GCG/PKA 信号抑制物质 P 的释放，从而缓解大鼠由顺铂引起的恶心和呕吐。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-15 DOI: 10.4014/jmb.2403.03044

Man Lu, Liwei Xie, Sijie Yin, Jing Zhou, Lingmei Yi, Ling Ye

{"title":"The Gut Microbial Lipid Metabolite 14(15)-EpETE Inhibits Substance P Release by Targeting GCG/PKA Signaling to Relieve Cisplatin-Induced Nausea and Vomiting in Rats.","authors":"Man Lu, Liwei Xie, Sijie Yin, Jing Zhou, Lingmei Yi, Ling Ye","doi":"10.4014/jmb.2403.03044","DOIUrl":"10.4014/jmb.2403.03044","url":null,"abstract":"Chemotherapy-induced nausea and vomiting (CINV) is a debilitating side effect related to activation of substance P (SP). SP activation can result from dysregulation of the gut-brain axis, and also from activation of protein kinase A signaling (PKA) signaling. In this study, we connected these factors in an attempt to unveil the mechanisms underlying CINV and develop new therapeutic strategies. Female rats were injected with cisplatin (Cis) to induce pica. Fecal samples were collected before/after injection, and subjected to lipid metabolomics analysis. In another portion of pica rats, the PKA inhibitor KT5720 was applied to investigate the involvement of PKA signaling in CINV, while fecal microbiota transplantation (FMT) was implemented to verify the therapeutic effect of the lipid metabolite 14(15)-EpETE. Pica symptoms were recorded, followed by ileal histological examination. The targeting relationship between 14(15)-EpETE and glucagon was determined by bioinformatics. SP and glucagon/PKA signaling in rat ileum, serum, and/or brain substantia nigra were detected by immunohistochemistry, enzyme-linked immunosorbent assay, and/or western blot. The results showed a significantly lower level of 14(15)-EpETE in rat feces after Cis injection. KT5720 treatment alleviated Cis-induced pica symptoms, ileal injury, SP content increase in the ileum, serum, and brain substantia nigra, and ileal PKA activation in rats. The ileal level of glucagon was elevated by Cis in rats. FMT exerted an effect similar to that of KT5720 treatment, relieving the Cis-induced changes, including ileal glucagon/PKA activation in rats. Our findings demonstrate that FMT restores 14(15)-EpETE production, which inhibits SP release by targeting GCG/PKA signaling, ultimately mitigating CINV.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1769-1777"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing the Effect of Microbial Consortia Fermentation on the Quality of HnB by Untargeted Metabolomics. 通过非靶向代谢组学分析微生物联合发酵对 HnB 质量的影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-30 DOI: 10.4014/jmb.2402.02039

Ling Zou, Hong Zhang, Zhonghua Liu, Jianfeng Sun, Yang Hu, Yishu Ding, Xinwei Ji, Zhenfei Yang, Qi Zhang, Binbin Hu

{"title":"Analyzing the Effect of Microbial Consortia Fermentation on the Quality of HnB by Untargeted Metabolomics.","authors":"Ling Zou, Hong Zhang, Zhonghua Liu, Jianfeng Sun, Yang Hu, Yishu Ding, Xinwei Ji, Zhenfei Yang, Qi Zhang, Binbin Hu","doi":"10.4014/jmb.2402.02039","DOIUrl":"10.4014/jmb.2402.02039","url":null,"abstract":"Fermentation has been identified as an effective strategy to alter the chemical makeup of tobacco, thereby enhancing its quality. The deliberate introduction of microorganisms can hasten the fermentation process. In this research, microbial consortia harvested from the tobacco surface were utilized to enhance the tobacco quality. This enhancement also elevated several sensory attributes of HnB cigarettes, such as aroma richness, moisture, strength, and reduced irritation, achieving a sensory quality rating of 84.5. This marks a notable improvement compared to the 82 rating of the original, unfermented cigarettes. Untargeted metabolomics analysis revealed a decrease in total polyphenols and unsaturated fatty acids, while the levels of polyacids, alcohols, ketones, furans, and other compounds increased in the fermented tobacco. Additionally, KEGG pathway enrichment analysis indicated that the enhancement in tobacco quality through microbial consortia fermentation is linked to various biological pathways, with pathways related to fatty acid and amino acid degradation playing pivotal roles. The findings of this study will serve as a reference for the commercial production of HnB cigarettes, and the elucidated mechanism offers a theoretical basis for exploring microbial fermentation as a means to improve tobacco quality.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1890-1897"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Insights into Cr(VI)-Induced Rhamnolipid Production and Gene Expression in Pseudomonas aeruginosa RW9 for Potential Bioremediation. 铜绿假单胞菌 RW9 中六价铬诱导的鼠李糖脂生产和基因表达的新见解，以实现潜在的生物修复。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-19 DOI: 10.4014/jmb.2406.06034

Fatini Mat Arisah, Norhayati Ramli, Hidayah Ariffin, Toshinari Maeda, Mohammed Abdillah Ahmad Farid, Mohd Zulkhairi Mohd Yusoff

{"title":"Novel Insights into Cr(VI)-Induced Rhamnolipid Production and Gene Expression in Pseudomonas aeruginosa RW9 for Potential Bioremediation.","authors":"Fatini Mat Arisah, Norhayati Ramli, Hidayah Ariffin, Toshinari Maeda, Mohammed Abdillah Ahmad Farid, Mohd Zulkhairi Mohd Yusoff","doi":"10.4014/jmb.2406.06034","DOIUrl":"10.4014/jmb.2406.06034","url":null,"abstract":"Rhamnolipid (RL) is renowned for its efficacy in bioremediating several types of organic and metal contaminants. Nevertheless, there has been a scarcity of studies specifically examining the relationship between this substance and metals, especially in terms of their impact on RL formation and the underlying interaction processes. This study addresses this gap by investigating the RL mechanism in Cr (VI) remediation and evaluating its effect on RL production in Pseudomonas aeruginosa RW9. In this study, P. aeruginosa RW9 was grown in the presence of 10 mg l-1 Cr (VI). We monitored RL yield, congeners distribution, and their ratios, as well as the transcriptional expression of the RL-encoded genes: rhlA, rhlB, and rhlC. Our results revealed that RL effectively reduced Cr (VI) to Cr (III), with RL yield increasing threefold, although with a slight delay in synthesis compared to control cells. Furthermore, Cr (VI) exposure induced the transcriptional expression of the targeted genes, leading to a significant increase in di-RL production. The findings confirm that Cr (VI) significantly impacts RL production, altering its structural compositions and enhancing the transcriptional expression of RL-encoded genes in P. aeruginosa RW9. This study represents a novel exploration of Cr (VI)'s influence on RL production, providing valuable insights into the biochemical pathways involved and supporting the potential of RL in Cr (VI) bioremediation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1877-1889"},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0