Journal of microbiology and biotechnology最新文献

{"title":"Potential Starter Candidate Based on Safety and Technological Evaluation of <i>Lactococcus lactis</i> from Kimchi, Korean Traditional Fermented Vegetables.","authors":"Yura Moon, Sojeong Heo, Gawon Lee, Sumin Lee, Minkyeong Kim, Moon-Hee Sung, Do-Won Jeong","doi":"10.4014/jmb.2501.01015","DOIUrl":"10.4014/jmb.2501.01015","url":null,"abstract":"<p><p>Continuous screening of suitable starter candidates for fermented foods necessary based on safety and technological evaluations. For this purpose, 34 <i>Lactococcus lactis</i> strains isolated from kimchi were evaluated to select potential starter candidates. All tested strains were susceptible to ampicillin, chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, and vancomycin. However, 32 strains exhibited resistance to streptomycin, while 2 showed resistance to tetracycline. None of the strains demonstrated hemolytic activity. All strains exhibited protease activity and acid production, but none displayed amylase or lipase activity. Notably, all strains exhibited antibacterial activity against <i>Listeria monocytogenes</i> ATCC 19111 and <i>Salmonella enterica</i> KCCM 11862. Furthermore, antibacterial activity was observed in 70.6%, 79.4%, 79.4%, 73.5%, and 32.4% of strains against <i>Bacillus cereus</i> KCCM 11341, <i>Enterococcus faecalis</i> KCTC 2011, <i>Staphylococcus aureus</i> ATCC 12692, <i>Flavobacterium</i> sp. KCCM 11374, and <i>Vibrio parahaemolyticus</i> KCTC 2729, respectively. Based on these assessments, <i>L. lactis</i> strain DMLL15 was identified as the most promising starter candidate. This strain was sensitive to nine antibiotics, exhibited non-hemolytic activity, demonstrated strong protease activity and acid production, and displayed antibacterial activity against seven pathogenic and spoilage bacteria. These findings support the safety assessment results and highlight the potential application of <i>L. lactis</i> DMLL15 as a starter candidate in the food industry.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501015"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Microbacterium chionoecetis</i> sp. nov. and <i>Agrococcus chionoecetis</i> sp. nov.: Novel Gut Bacteria from Red Snow Crab.","authors":"Dhiraj Kumar Chaudhary, Sang-Eon Kim, Hye-Jin Park, Kyoung-Ho Kim","doi":"10.4014/jmb.2412.12044","DOIUrl":"10.4014/jmb.2412.12044","url":null,"abstract":"<p><p>Two yellow-coloured, Gram-stain-positive, oxidase-negative, aerobic, non-motile, and rod-shaped strains, labelled as ProA8^T and ProA11^T, were isolated from digestive tract of red snow crab (<i>Chionoecetes japonicus</i>). Strain ProA8^T grow at temperature range of 15-35°C, while strain ProA11^T grow at temperature range of 15-40°C. Phylogenetic and 16S rRNA gene sequence analysis revealed that strains ProA8^T and ProA11^T belonged to the genera <i>Microbacterium</i> and <i>Agrococcus</i>, respectively. Strain ProA8^T was closely affiliated with <i>Microbacterium yannicii</i> JCM 18959^T (98.8%) and strain ProA11^T was most closely related to <i>Agrococcus baldri</i> IAM 15147^T (98.9%). The genome sizes of ProA8^T and ProA11^T were 4,373,776 bp and 2,665,899 bp, with DNA G+C contents of 70.5% and 70.1%, respectively. The genomic relatedness values of ProA8^T and ProA11^T with their respective reference strains were <32.0% (for digital DNA-DNA hybridization) and <87.0% (for average nucleotide identity). Biosynthetic gene analysis showed the presence of genes for resorcinol production in ProA8^T and ectoine biosynthesis in ProA11^T, indicating ecological and biotechnological significance. Virulence analyses determined that strains ProA8^T and ProA11^T were non-pathogenic to humans, highlighting their safe application in biotechnological field. The major cellular fatty acids reported in ProA8^T and ProA11^T were anteiso-C_15:0, iso-C_16:0, and anteiso-C_17:0. Overall, the polyphasic taxonomic data suggest that both strains ProA8^T and ProA11^T represent two novel species within the genera <i>Microbacterium</i> and <i>Agrococcus</i>, respectively. Accordingly, we propose the names <i>Microbacterium chionoecetis</i> sp. nov., with the type strain ProA8^T (= KCTC 49861^T = JCM 37392^T) and <i>Agrococcus chionoecetis</i> sp. nov., with the type strain ProA11^T (= KCTC 49958^T = JCM 37393^T).</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412044"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xylose Metabolism and Transport in <i>Bacillus subtilis</i> and Its Application to D-Ribose Production.","authors":"Yong-Cheol Park","doi":"10.4014/jmb.2504.04021","DOIUrl":"https://doi.org/10.4014/jmb.2504.04021","url":null,"abstract":"<p><p>Xylose is a five-carbon sugar and the second abundant mono-saccharide in lignocellulosic biomass. Xylose is not only a sugar substitute by itself, but also a good carbon source for the microbial and enzymatic synthesis of various valuable biomaterials. Most microorganisms are able to uptake and consume xylose as a sole carbon source because they possess specific transport systems and metabolic enzymes. <i>Bacillus subtilis</i> is a representative Gram-positive bacterium commercially used for enzyme and food production. Even though <i>B. subtilis</i> is popular in genetic and protein engineering, its application for metabolic engineering has been limited. Meanwhile, D-ribose is a five-carbon sugar and essential component in nucleotides, ATP, NAD, coenzyme A and so on. It boosts healthy effects on the human body such as enhancement of muscle performance and tolerance to myocardial ischemia. To produce D-ribose from xylose in <i>B. subtilis</i>, a comprehensive review on xylose metabolic regulation, xylose transport, and D-ribose biosynthetic engineering and fermentation process was provided. It would be useful for production of other valuable metabolites from xylose in <i>B. subtilis</i>.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504021"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12034463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Rapid On-Site Method for the Detection of <i>Kazachstania servazzii</i> and <i>Candida sake</i> in Kimchi Using Loop-Mediated Isothermal Amplification.","authors":"Yoon-Soo Gwak, Gyeong-Eun Kim, Chan-Il Bae, Hae-Yeong Kim, Mi-Ju Kim","doi":"10.4014/jmb.2502.02029","DOIUrl":"10.4014/jmb.2502.02029","url":null,"abstract":"<p><p>Kimchi has recently gained considerable global interest for its health benefits, including its probiotic properties and anticancer and antioxidative effects. However, in the later stages of kimchi fermentation, an increase in the number of yeast species that form a white film on the surface of kimchi, such as <i>Kazachstania servazzii</i> and <i>Candida sake</i>, takes place, which can negatively affect kimchi quality. Thus, we developed loop-mediated isothermal amplification (LAMP) assays for the on-site detection of <i>K. servazzii</i> and <i>C. sake</i> in kimchi. The internal transcribed spacer (ITS) region of 24 yeast strains were aligned to identify the specific region of the target yeasts, after which the LAMP primer for each target species was designed. The developed LAMP primer sets specifically amplified <i>K. servazzii</i> and <i>C. sake</i> and detected 0.4 and 4 pg of <i>K. servazzii</i> and <i>C. sake</i> DNA, respectively. In addition, the limits of detection were 10³ CFU/ml for <i>K. servazzii</i> and 10² CFU/ml for <i>C. sake</i>, respectively. The LAMP methods were validated by applying them to fermented kimchi samples, with the LAMP reactions being completed within 35 min. Overall, we found that the developed LAMP assays had excellent potential to be used for advanced on-site detection of <i>K. servazzii</i> and <i>C. sake</i> due to their specificity, sensitivity, and rapidity.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502029"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive Evolution of GatC, a Component of the Galactitol Phosphotransferase System, for Glucose Transport in <i>Escherichia coli</i>.","authors":"Su On Jeong, Hyun Ju Kim, Sang Jun Lee","doi":"10.4014/jmb.2502.02021","DOIUrl":"10.4014/jmb.2502.02021","url":null,"abstract":"<p><p>Microbial adaptive laboratory evolution is a powerful approach for uncovering novel gene functions within metabolic pathways. Building on our previous discovery of ExuT as a glucose transporter in <i>ptsG</i>-deficient <i>Escherichia coli</i>, this study investigates strains lacking recognized glucose transporters (<i>ptsG</i>, <i>manX</i>, and <i>exuT</i>). Successive rounds of experimental evolution revealed key genetic adaptations, including loss-of-function mutations in <i>malI</i> and <i>nagC</i>, which encode repressors of the maltose and N-acetylglucosamine phosphotransferase systems (PTS), respectively. Additionally, a gain-of-function mutation in <i>gatC</i>, a component of the galactitol PTS EIIC, was identified. The functional significance of these mutations was validated through transcript analysis, genetic knockouts, and CRISPR-Cas9-mediated site-specific genome mutagenesis, with a particular focus on the <i>gatC</i> missense mutation (F340C). The resulting modifications were examined for their effects on sugar specificity and metabolic flux. Furthermore, our findings identified succinate as the predominant fermentation product in engineered strains utilizing alternative glucose transport pathways, including the maltose, N-acetylglucosamine, and galactitol PTS. This study advances our understanding of sugar transport mechanisms in <i>E. coli</i> and offers insights into regulatory networks, fermentative metabolism, and substrate specificity, which can be leveraged for evolutionary engineering in biotechnological applications.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502021"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of the First Shuttle Vector System and Optimization of Transformation in <i>Selenomonas sputigena</i>.","authors":"Trinh Thi Nguyen, Yu-Kyung Kim, Duhyun Ko, Jeeyou Kim, Hana Yi, Ye-Ji Bang","doi":"10.4014/jmb.2501.01011","DOIUrl":"10.4014/jmb.2501.01011","url":null,"abstract":"<p><p><i>Selenomonas sputigena</i>, an anaerobic bacterium found in the human respiratory tract, has recently gained significant attention due to its dual role in human health - as both a periodontal pathogen and a protective agent against severe asthma. However, the absence of genetic tools for this organism has severely limited our understanding of its molecular mechanisms and therapeutic potential. Here, we report the first successful development of a genetic modification system for <i>S. sputigena</i> ATCC 35185. We constructed a shuttle vector carrying the Rep191 replication protein from <i>Selenomonas ruminantium</i> and systematically optimized transformation conditions. Through careful optimization of key parameters including DNA quantity, bacterial growth phase, membrane permeabilization, and post-pulse recovery time, we achieved a 22.5-fold improvement in transformation efficiency (from ~2,000 to ~45,000 CFU/μg DNA). The broad applicability of our system was demonstrated through successful transformation of multiple <i>Selenomonas</i> species, establishing the first standardized genetic modification system for this genus. We further validated the system's functionality by achieving stable GFP expression in <i>S. sputigena</i>, representing the first demonstration of ectopic protein expression in this organism. This work provides essential tools for investigating the molecular basis of <i>S. sputigena</i>'s therapeutic effects and pathogenic capabilities, potentially accelerating the development of novel microbiome-based treatments for both respiratory and oral diseases.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501011"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin Inhibits Colorectal Cancer associated with a High-Fat Diet through Modulation of Gut Microbiota and IL-6/STAT3 Pathway.","authors":"Jiahui Sun, Luyao Shi, Fan Xu, Hanyan Sun, Yitong Liu, Jiangyun Sun, Qingxin Zhou","doi":"10.4014/jmb.2412.12029","DOIUrl":"10.4014/jmb.2412.12029","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a worldwide health issue. It causes illness and death in millions of people each year. A positive correlation has been observed between the intake of dietary fat and the development of CRC. The composition of gut microbiota exhibits a significant correlation with pathophysiologic processes in intestine. Clinical treatment remains inadequate due to the complex pathogenic mechanisms of CRC triggered by a high-fat diet (HFD). Naringenin, a flavonoid from grapefruit, has anti-cancer activity. Our findings suggest that naringenin enhances gut microbiota diversity by increasing the abundance of beneficial bacterial species while reducing opportunistic pathogenic bacteria. The fecal microbiota transplantation assay (FMT) demonstrated that the anti-HFD-CRC activity of naringenin depended on the gut microbiota. Furthermore, naringenin antagonized the IL-6/STAT3 pathway. These results suggest that naringenin may be a potential treatment for HFD-CRC.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412029"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering <i>Escherichia coli</i> for Anaerobic Succinate Fermentation Using Corn Stover Hydrolysate as a Substrate.","authors":"Haining Yang, Yali Dong","doi":"10.4014/jmb.2412.12041","DOIUrl":"10.4014/jmb.2412.12041","url":null,"abstract":"<p><p>Succinic acid is regarded as one of the most important platform chemicals used in materials science, chemistry, and food industrial applications. Currently, the main bottlenecks in the microbial succinate synthesis lie in the low titer, cofactor imbalance, and high production costs. To overcome these challenges, the reductive tricarboxylic acid cycle (TCA) and glucose uptake pathway were enhanced, increasing the titer of succinate to 4.31 g/l, 2.06-fold of the original strain. Furthermore, formate dehydrogenase from <i>Candida boidinii</i> was simultaneously overexpressed to increase the regeneration of NADH which was deficient in succinate synthesis under anaerobic condition. On this basis, the oxygen-responsive biosensor was used to replace the isopropyl-β-d-thiogalactoside (IPTG)-induction system, enabling strain to avoid the utilization of IPTG for succinate production. Using corn stover hydrolysate as the substrate, the optimum strain produced 60.74 g/l succinate in 5 L bioreactor. The engineered strain exhibited high succinate titer using biomass hydrolysate as substrate, significantly reduced the fermentation cost.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412041"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of SAM-Dependent Methyltransferases from <i>Halomonas</i> sp. YLGW01 on Phospholipid Fatty Acids Composition and Production of Polyhydroxalkanoates in <i>Escherichia coli</i>.","authors":"Tae-Rim Choi, Gaeun Lim, Yebin Han, Jong-Min Jeon, Jeonghee Yun, Jeong-Jun Yoon, Shashi Kant Bhatia, Yung-Hun Yang","doi":"10.4014/jmb.2412.12065","DOIUrl":"10.4014/jmb.2412.12065","url":null,"abstract":"<p><p>The bacterial membrane changes in response to growth conditions such as salt concentration, temperature, and growth inhibitors. As membrane fatty acid related genes are important in controlling the membrane composition, we studied two genes <i>i.e.</i> cyclopropane fatty acid synthase (<i>cfa</i>) and cis-vaccinate 11-methyltransferase (<i>ufaM</i>) that designated to S-adenosylmethionine (SAM)-dependent methyltransferase derivatives from <i>Halomonas</i> sp. YLGW01. These two genes are related to a response to external growth conditions by controlling the fluidity of membrane changes. The role of these two genes (<i>cfa</i> and <i>ufaM</i>) was accessed by overexpression in <i>Escherichia coli</i> and results showed an evident increase in the levels of cyclic fatty acid and detection of furan fatty acid intermediate analyzed by Gas chromatography Mass spectrometer (GC-MS), respectively, resulting in changes of phospholipid fatty acids (PLFA) and membrane properties. Overexpression of these genes in engineered polyhydroxyalkanoate (PHA) producing <i>E. coli</i> strain promoted the bacterial growth (1.6-fold) and PHA accumulation (2-fold). Overall, this study showed two membrane fatty acid synthases, <i>ufaM</i> and <i>cfa</i> able to change membrane fatty acid composition and have the potential to improve cell's robustness and PHA production capability.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412065"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Evaluation of Synthetic Microbial Communities for Effective Fire Blight Disease Control in Apples.","authors":"Yejin Lee, Da-Ran Kim, Youn-Sig Kwak","doi":"10.4014/jmb.2501.01047","DOIUrl":"https://doi.org/10.4014/jmb.2501.01047","url":null,"abstract":"<p><p>Fire blight disease caused by <i>Erwinia amylovora</i> has resulted in extreme economic losses to industrial plants of the Rosaceae family, including apples and pears, since the 1870s. Many countries have used pesticides and bactericides to manage the fire blight disease. However, chemical management leads to the emergence of a pesticide-resistant pathogen population. Therefore, attempts at managing the fire blight disease have been developed and applied using selected microorganisms as a biological control. However, a single strain of the biological control agent showed limited effect in reducing the disease. Here, we designed synthetic microbial communities (SynCom), which involve reconstituting multi-strains rather than relying on a single strain to enhance the disease control efficiency. We constructed the SynCom based on three categorized functions: i) anti-EA strain, ii) keystone taxa in healthy apples, iii) abundant metabolite strain in healthy apples than the fire blight infected apples. SynCom was significantly efficient in suppressing fire blight, achieving 0% disease severity in apple fruits, 1.67% in roses, and 5.4% in apple plants. Our finding presented that the well-designed SynCom showed significant effects in controlling the fire blight disease. Additionally, SynCom members should be selected based on multiple functions to maximize the impact on crop management.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501047"},"PeriodicalIF":2.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}