Journal of microbiology and biotechnology最新文献

{"title":"<i>Akkermansia muciniphila</i> Alleviates Sarcopenia in Senescence-Accelerated Mouse-Prone 8 Mice.","authors":"So-Hyun Park, Hee Soo Kim, Pyeong Geun Choi, Myeong Seon Jeong, Yang Hoon Huh, Jiyun Ahn, Chang Hwa Jung","doi":"10.4014/jmb.2507.07001","DOIUrl":"10.4014/jmb.2507.07001","url":null,"abstract":"<p><p><i>Akkermansia muciniphila</i>, an intestinal bacterium, has garnered attention for its association with metabolic health and anti-inflammatory properties. However, its potential role in mitigating sarcopenia, particularly in the senescence-accelerated mouse-prone 8 (SAMP8) model, remains unexplored. In this study, we aimed to evaluate the potential effects of <i>A. muciniphila</i> supplementation on sarcopenia and its underlying mechanisms. Seven-month-old SAMP8 mice were administered <i>A. muciniphila</i> for 3 months. <i>A. muciniphila</i> supplementation enhanced grip strength and skeletal muscle mass, suppressed cellular senescence, improved the balance between protein degradation and synthesis, increased total ATP, and improved mitochondrial biogenesis. Analysis of the effects of <i>A. muciniphila</i> on gut microbiome using 16S rRNA sequencing showed that supplementation with <i>A. muciniphila</i> shifted the gut microbiota composition, alleviated gut dysbiosis, preserved gut barrier integrity, and reduced the protein expression of inflammatory cytokines in the intestine. Additionally, extracellular vesicles derived from <i>A. muciniphila</i> promoted myogenesis and suppressed dexamethasone-induced atrophy in C2C12 myoblasts. These findings suggest that supplementation with <i>A. muciniphila</i> mitigates sarcopenia by suppressing inflammation and improving the gut microenvironment, highlighting the potential of <i>A. muciniphila</i> as a new therapeutic candidate to treat sarcopenia.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507001"},"PeriodicalIF":3.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory Effects of LSI312A on Dendritic Cells: A Novel Approach to Modulating Inflammatory Pathways.","authors":"Hien Thi Thu Do, Chaelin Lee, Inmoo Rhee","doi":"10.4014/jmb.2506.06027","DOIUrl":"10.4014/jmb.2506.06027","url":null,"abstract":"<p><p>Inflammation plays a crucial role in the pathogenesis of various diseases, necessitating the development of effective anti-inflammatory therapeutics. Dendritic cells (DCs), as professional antigen-presenting cells, are key regulators of immune responses. In this study, we investigated the immunomodulatory effects of LSI312A, a novel compound derived from medicinal plant analogues, on DC function and inflammatory signaling pathways. LSI312A exhibited no cytotoxicity in DC2.4 cells at concentrations up to 20 μM. LSI312A significantly reduced antigen uptake and impaired the expression of co-stimulatory molecules, particularly MHC class II and CD40, upon lipopolysaccharide (LPS) stimulation. Moreover, LSI312A markedly suppressed the secretion of pro-inflammatory cytokines, including TNF-α and IL-6, and decreased nitric oxide (NO) production by downregulating iNOS expression at both the mRNA and protein levels. Mechanistically, LSI312A inhibited the phosphorylation of NF-κB, a central regulator of inflammatory responses, while promoting Nrf2 nuclear translocation, an essential factor in antioxidant signaling. Furthermore, LSI312A effectively suppressed the activation of the PI3K/Akt pathway, contributing to its anti-inflammatory effects. These results suggest that LSI312A modulates key inflammatory pathways and DC-mediated immune responses, highlighting its potential as a novel therapeutic candidate for inflammation-related diseases.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506027"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Research Progress on the Microbial or Enzymatic Degradation and Mechanism of Aflatoxin B1.","authors":"Aiyuan Zhang, Jiguo Yang","doi":"10.4014/jmb.2504.04044","DOIUrl":"10.4014/jmb.2504.04044","url":null,"abstract":"<p><p>Aflatoxins (AFs) are secondary metabolites produced by toxigenic <i>Aspergillus</i> species, which are highly toxic and exhibit carcinogenic, mutagenic, and teratogenic properties, posing a significant threat to human and animal health. Cereals and their products are the primary targets for aflatoxin-producing fungal contamination, causing substantial losses to the livestock and food industries. Due to the high toxicity and widespread contamination of aflatoxin B1 (AFB1), the prevention and degradation of AFs in cereals and their products are essential. This review summarizes the AFB1-degrading bacteria, such as <i>Bacillus</i>, <i>Actinomycetes</i>, and <i>γ-Proteobacteria</i>, as well as fungi, including <i>Aspergillus</i> and <i>Basidiomycota</i>. It also provides an overview and discussion of the chemical structures and toxicity of the degradation products. Additionally, the review delves into the possible oxidative, reductive, and hydrolytic mechanisms of AFB1 enzymatic degradation. The aim of this study is to provide a reference for the biological degradation of AFB1 and to promote further research in this area.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504044"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Risk of Microplastics on Gill and Gut Health and Subsequent Pathogen Susceptibility in the Goldfish Model.","authors":"Ho Sung Kim, Bohyun Yun, Yongjoon Yoon, Jeong Woo Park, Jimin Hyun, BoMi Ryu, Aaron M Yerke, Sungmin Hwang, Ki Hwan Moon","doi":"10.4014/jmb.2504.04019","DOIUrl":"10.4014/jmb.2504.04019","url":null,"abstract":"<p><p>Microplastics are pervasive pollutants in aquatic ecosystems, yet their effects on fish tissues remain insufficiently characterized. Our study investigates the impact of polystyrene microplastics (0.5 and 2 μm) on the gill and intestinal tissues of goldfish (<i>Carassius auratus</i>), with a focus on inflammatory responses and pathogen susceptibility. Following two weeks of exposure, histological and molecular analyses revealed increased filament cartilage thickness in gills, enhanced villus thickness and goblet cell numbers in intestines, and upregulation of immune- and oxidative stress-related genes. Exposure to 0.5 μm microplastics significantly reduced survival after <i>Edwardsiella piscicida</i> infection, indicating increased vulnerability to pathogens. These findings highlight the immunotoxic effects of microplastics and their potential to compromise fish health in contaminated environments.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504019"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"8-Methoxybicolosin C from <i>Lespedeza bicolor</i> Attenuates Inflammation and Oxidative Stress via Nrf2/HO-1 and NF-κB/MAPK Pathways in Lipopolysaccharide-Induced Mouse Kupffer Cells.","authors":"Young-Chang Cho, Lulu Yao, Da Young Lee, Xiangying Li, Guijae Yoo, Sang Yoon Choi, Namki Cho, Su-Jin Park, Somy Yoon, Jae Sung Lim","doi":"10.4014/jmb.2503.03013","DOIUrl":"10.4014/jmb.2503.03013","url":null,"abstract":"<p><p><i>Lespedeza bicolor</i> (<i>L. bicolor</i>) is known for its anti-inflammatory, antioxidant, and anticancer properties, making it a common choice in traditional medicine practices. Researchers in several recent studies have focused on isolating individual phytochemicals from this plant through chromatography analysis to explore their therapeutic potential. In our previous work, we identified 8-methoxybicolosin C (8-MC) as a novel flavonoid derivative, isolated and purified from the roots of <i>L. bicolor</i>, which exhibited inhibitory effects on cell proliferation. In this study, we further investigated the biological activities of 8-MC by examining its antioxidant and anti-inflammatory effects in LPS-induced mouse Kupffer cells. The results showed that 8-MC suppresses the expression of inflammation-related mediators, including inducible nitric oxide synthase (iNOS), nitric oxide (NO), and pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β, in a dose-dependent manner. Additionally, 8-MC improves the GSH/GSSG balance by increasing glutathione (GSH) levels and decreasing oxidized glutathione (GSSG) levels. Interestingly, 8-MC was found to bind Keap1, preventing roteasomal degradation, and promoting the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), thereby increasing the expression of antioxidant-related proteins such as heme oxygenase-1 (HO-1). Moreover, 8-MC suppressed the activation of inflammatory signaling pathways, including c-Jun N-terminal kinases (JNKs) and p38 mitogen-activated protein kinases (MAPKs), while also inhibiting the nuclear translocation of nuclear factor kappa B (NF-κB), effectively reducing inflammatory responses. These findings collectively demonstrated that 8-MC possesses potent anti-inflammatory and antioxidant activities through the regulation of NF-κB, MAPK, and Nrf2/HO-1 signaling pathways. Consequently, 8-MC shows potential as a valuable therapeutic agent for managing various inflammatory disorders.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503013"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Domestic Alternative Positive Control Strain to <i>Bacillus cereus</i> ATCC 14579 for Microbial Assays.","authors":"Su-Hyeon Joung, Yu-Si Lee, Byeong Joon Kim, Yongchjun Park, Seung Hwan Kim, Soon Han Kim, Insun Joo, Eun Sook An","doi":"10.4014/jmb.2504.04003","DOIUrl":"10.4014/jmb.2504.04003","url":null,"abstract":"<p><p>Most positive control strains in microbial assays are sourced from abroad. <i>Bacillus cereus</i> ATCC 14579 is used as the positive control strain in microbial assays described in the Manual for the Detection of Foodborne Pathogens at Outbreaks and the FDA's Bacteriological Analytical Manual method. This study aimed to identify a domestically isolated strain from the Ministry of Food and Drug Safety (MFDS) to replace <i>B. cereus</i> ATCC 14579. A total of 323 strains from the MFDS were collected, and gene-targeted polymerase chain reaction and biochemical tests were performed to characterize candidate strains. Sixty-one candidate strains showing identical biochemical and molecular characteristics to ATCC 14579 were further subjected to whole-genome sequencing. Comparative genetic analyses using MLST, SNP distance, and OrthoANI revealed that <i>B. cereus</i> MFDS 1004972 shared the same sequence type, exhibited SNP distance of fewer than 10 SNPs, and showed 100% ANI with ATCC 14579. Therefore, <i>B. cereus</i> MFDS 1004972 is proposed as a suitable domestic alternative to the imported strain.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504003"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review on Current Research on Biosynthesis of Biosurfactants and the Regulation Influenced by Metal Ions.","authors":"Yuchen Wang, Shan Qiao, Yongwu Niu","doi":"10.4014/jmb.2503.03031","DOIUrl":"10.4014/jmb.2503.03031","url":null,"abstract":"<p><p>Biosurfactants, amphiphilic secondary metabolites mainly produced by microorganisms, can be categorized into five groups according to their chemical structure and source: glycolipids, lipopeptides, phospholipids, polymeric biosurfactants, and particulate surfactants. The hydrophobic segments of biosurfactants typically contain fatty acids of varying chain lengths, while their hydrophilic portions display a wide range of diversity. Diverse biosurfactants have distinct metabolic pathways. Glycolipids are usually associated with glycolysis/gluconeogenesis and fatty acid metabolism, while lipopeptides are closely connected to non-ribosomal peptide synthetase. Particulate biosurfactants are formed through the anabolic pathway of phospholipids, with amino acid metabolism and carbohydrate metabolism being crucial components in the process of creating polar head groups. Metal ions are critical for either directly influencing strain growth or governing genes connected to biosurfactants production. This review explores the synthesis pathways of various biosurfactants and examines the influence of different metal ions on their production.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503031"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refining Dendritic Cell-Based Cancer Vaccines: Subset Targeting, Translational Barriers, and Emerging Strategies.","authors":"Inmoo Rhee","doi":"10.4014/jmb.2506.06021","DOIUrl":"10.4014/jmb.2506.06021","url":null,"abstract":"<p><p>Dendritic cells (DCs) are pivotal regulators of immune responses, capable of initiating robust adaptive immunity through antigen presentation. As the most potent antigen-presenting cells, they have emerged as central components of cancer immunotherapy. Over the last decade, advances in molecular engineering, bioinformatics, and nanomedicine have transformed the design of DC-based vaccines. Strategies now include personalized neoantigen loading, mRNA-electroporation, nanoparticle-mediated delivery, and combinatorial regimens with immune checkpoint inhibitors. In addition, emerging approaches that target DC subsets <i>in vivo</i>, especially cDC1s, have demonstrated enhanced efficacy in preclinical and early clinical studies. This review provides a comprehensive overview of the biological roles of DCs and evaluates the evolution of DC vaccine platforms while also highlighting new technologies and clinical insights that aim to tumor-induced immunosuppression suppression and improve therapeutic outcomes.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506021"},"PeriodicalIF":3.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Purification of Antibacterial Compound from Kombucha of SCOBY.","authors":"Younhee Nam, Junghoon Lee, Soo Rin Kim, Jong Nam Kim","doi":"10.4014/jmb.2504.04012","DOIUrl":"10.4014/jmb.2504.04012","url":null,"abstract":"<p><p>The misuse of antibiotics has contributed to the widespread emergence of antimicrobial resistance (AMR), emphasizing the need for alternative antimicrobial agents. Kombucha, a fermented beverage containing a symbiotic culture of bacteria and yeast (SCOBY), has gained attention for its antibacterial activity and potential health benefits. This study investigated the antibacterial properties of kombucha and SCOBY, isolating and characterizing the active compounds responsible for these effects. Both kombucha broth and dried SCOBY effectively inhibited <i>Escherichia coli</i>, <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, and <i>Salmonella Typhimurium</i>, with dried SCOBY demonstrating stronger activity. Instrumental analyses identified 5-hydroxymethylfurfural (HMF) as the primary antibacterial compound in the SCOBY extracts. HMF significantly inhibited <i>L. monocytogenes</i> and <i>S. aureus</i>, with its antibacterial inhibition surpassing that of chloramphenicol in these two bacterial species. Previous studies have shown that, in addition to its antibacterial effects, HMF has potential applications in the production of polymers and pharmaceuticals, and as a fuel additive, suggesting its potential in the chemical and biofuel industries. This study highlights the antibacterial activity of HMF and underscores the need for further research to evaluate its safety and applicability in various fields.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504012"},"PeriodicalIF":3.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reviving Furosemide as a Metallo-β-Lactamase Inhibitor against MDR <i>Acinetobacter baumannii</i>.","authors":"Hanan Al-Lawati, Fatma Al-Raisi, Mahmoud A Elfaky, Mahmoud Saad Abdel-Halim, Hisham A Abbas, Basem Mansour, Wael A H Hegazy, Noura M Seleem","doi":"10.4014/jmb.2506.06023","DOIUrl":"10.4014/jmb.2506.06023","url":null,"abstract":"<p><p>Carbapenems are considered the last line of antibiotic defense against multidrug-resistant (MDR) <i>Acinetobacter baumannii</i>, a bacterium that can secrete carbapenemases such as metallo-β-lactamases (MBLs) to degrade carbapenems. It is thus critical to develop strategies to combat carbapenem resistance, and one of these strategies is to discover MBL inhibitors. In the current study, we evaluated the possible anti-β-lactamase of the approved safe drug furosemide. Furosemide is a loop diuretic with antihypertensive effects. A clinical MDR and carbapenem-resistant <i>A. baumannii</i> isolate was used. Furosemide at 1 mg/ml potentiated meropenem in the combined disk method and interfered with the hydrolytic activities of MBL. Furthermore, furosemide synergized meropenem and decreased its minimum inhibitory concentration (MIC). Furosemide could also reduce the expression of the MBL genes <i>bla</i>_NDM and <i>bla</i>_VIM. In silico study showed the binding ability of furosemide with the active sites of New Delhi and VIM MBL enzymes, as well as its chelating interaction with zinc ions. Furosemide is a promising MBL inhibitor that can be used in combination with meropenem to treat infections caused by MBL-producing <i>A. baumannii</i>.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506023"},"PeriodicalIF":3.1,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}