Journal of microbiology and biotechnology最新文献_第2页

Cutaneous UVB Irradiation Alters Gut Microbiota Independently of Skin Inflammation in SKH-1 Hairless Mice. 皮肤UVB照射改变SKH-1无毛小鼠肠道微生物群独立于皮肤炎症。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-22 DOI: 10.4014/jmb.2507.07020

Eunjung Lee, Woo-Jin Sim, Tae-Gyu Lim

{"title":"Cutaneous UVB Irradiation Alters Gut Microbiota Independently of Skin Inflammation in SKH-1 Hairless Mice.","authors":"Eunjung Lee, Woo-Jin Sim, Tae-Gyu Lim","doi":"10.4014/jmb.2507.07020","DOIUrl":"https://doi.org/10.4014/jmb.2507.07020","url":null,"abstract":"This study investigates the impact of chronic ultraviolet B (UVB) exposure on gut microbiota composition and skin inflammation. Mice were subjected to a 12-week regimen of low-dose UVB irradiation designed to mimic mild photoaging. Although no significant changes were observed at the phylum level, UVB exposure resulted in a significant decrease in the genus Lactobacillus (P=0.0302) and an increase in Duncaniella (P=0.046), accompanied by a reduction in α-diversity. At the species level, Lactobacillus johnsonii, a known probiotic, was significantly decreased, while Duncaniella freteri was increased in the UVB-exposed group. Despite these microbial alterations, no significant skin inflammation was detected, as mRNA levels of interleukin (IL)-1β and TNF-α and protein levels of IL-1β and IL-2 remained unchanged. These findings suggest that chronic low-dose UVB exposure can alter gut microbial homeostasis independently of local inflammatory responses, highlighting a potential role for the skin-gut axis in mediating systemic effects of environmental UVB exposure.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507020"},"PeriodicalIF":3.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Benefits of Low-Molecular-Weight Collagen Peptide in Achilles Tendon and Medial Collateral Ligament Injuries and Anterior Cruciate Ligament Transection-Induced Osteoarthritis. 低分子胶原肽在跟腱、内侧副韧带损伤和前交叉韧带横断性骨关节炎中的功能益处。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-22 DOI: 10.4014/jmb.2506.06035

Eunjung Choi, Myunghee Kim, Dae-Hyo Lee, Sehwan Shim, Do-Un Kim, Jeong-Min Hong, Hee-Chul Chung

{"title":"Functional Benefits of Low-Molecular-Weight Collagen Peptide in Achilles Tendon and Medial Collateral Ligament Injuries and Anterior Cruciate Ligament Transection-Induced Osteoarthritis.","authors":"Eunjung Choi, Myunghee Kim, Dae-Hyo Lee, Sehwan Shim, Do-Un Kim, Jeong-Min Hong, Hee-Chul Chung","doi":"10.4014/jmb.2506.06035","DOIUrl":"https://doi.org/10.4014/jmb.2506.06035","url":null,"abstract":"Musculoskeletal disorders, such as Achilles tendinopathy and anterior cruciate ligament (ACL) injury, can cause serious impairments in physical function and daily life. In particular, ACL rupture increases the risk of osteoarthritis (OA). Collagen maintains the mechanical strength and resilience of tendons and articular cartilage, while low-molecular-weight collagen peptides (LMWCP) support tissue integrity and joint mobility. This study evaluated the efficacy of LMWCP in promoting the repair of Achilles tendon (AT) and medial collateral ligament (MCL), and preventing ACL transection (ACLT)-induced OA. In animal models, LMWCP was administered following AT and MCL injuries, as well as ACLT. LMWCP treatment in the ACLT rabbit model significantly improved cartilage integrity, reducing surface damage, proteoglycan loss, and OARSI scores. It also inhibited subchondral bone deterioration and osteophyte formation, restoring bone volume fraction, as shown by Micro-CT analysis. Moreover, LMWCP administration increased type II collagen expression while decreasing that of matrix metalloproteinases (MMPs) in cartilage tissue, and decreased inflammatory cytokine concentrations in the synovial fluid. In IL-1β-stimulated human chondrocytes, LMWCP suppressed inflammatory cytokines and MMP expression, and increased hydroxyproline content, indicating reduced collagen degradation. In the AT and MCL defect model, LMWCP promoted tissue regeneration by improving fiber arrangement, rounding of nuclei, angiogenesis, and cell density and promoting collagen deposition. These findings suggest that LMWCP may enhance joint function and tendon healing through modulation of inflammation and collagen remodeling, representing a functional ingredient for promoting tendon and ligament repair and attenuating ACLT-induced OA progression.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506035"},"PeriodicalIF":3.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the Purification Process for Lactiplantibacillus plantarum Lipoteichoic Acid with Anti-Biofilm Properties against Dental Pathogens. 具有抗口腔病原菌生物膜特性的植物乳杆菌脂壁酸纯化工艺优化

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-22 DOI: 10.4014/jmb.2506.06045

Jeongmin Shin, Dong Hyun Park, Woohyung Jun, Ok-Jin Park, Cheol-Heui Yun, Jintaek Im, Seung Hyun Han

{"title":"Optimization of the Purification Process for Lactiplantibacillus plantarum Lipoteichoic Acid with Anti-Biofilm Properties against Dental Pathogens.","authors":"Jeongmin Shin, Dong Hyun Park, Woohyung Jun, Ok-Jin Park, Cheol-Heui Yun, Jintaek Im, Seung Hyun Han","doi":"10.4014/jmb.2506.06045","DOIUrl":"https://doi.org/10.4014/jmb.2506.06045","url":null,"abstract":"Biofilms formed by oral pathogens play a critical role in the initiation and development of various dental diseases by enhancing resistance to dental medicaments. Previously, we reported the potent anti-biofilm activity of Lactobacillus lipoteichoic acid (LTA), a major cell wall component of Gram-positive bacteria, against various dental pathogens. Nevertheless, the practical application of LTA as an anti-biofilm agent is limited due to its complex, cost- and labor-intensive purification processes. Therefore, to minimize the purification processes required to obtain LTA with anti-biofilm activity, we isolated LTA from Lactiplantibacillus plantarum (Lp.LTA) and its intermediates following each purification step, and compared their anti-biofilm activity against dental pathogens. The Lp.LTA intermediates underwent sequential purification after butanol extraction and hydrophobic-interaction chromatography, and these were designated as LTA-Butanol and LTA-HIC, respectively. The results of Western blot analysis demonstrated that LTA-HIC has a higher concentration of LTA than LTA-Butanol. Although both LTA-Butanol and LTA-HIC dose-dependently inhibited Streptococcus mutans and Porphyromonas gingivalis biofilm formation, the anti-biofilm activity of LTA-HIC was superior to that of LTA-Butanol and comparable to Lp.LTA. Furthermore, sodium hydroxide treatment of LTA-HIC diminished its anti-biofilm activity, suggesting that LTA is a key component responsible for the anti-biofilm capacity of LTA-HIC. Collectively, these results demonstrated that LTA-HIC serves as an intermediate in the LTA purification, retaining its anti-biofilm properties and offering a viable solution to the challenges associated with the LTA purification process.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506045"},"PeriodicalIF":3.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-Theanine Ameliorates Metabolic Dysregulation and Adverse Fetal Outcomes in a Mice Model of Gestational Obesity: Association with FXR/FGF15 Signaling. l -茶氨酸改善妊娠肥胖小鼠模型中的代谢失调和不良胎儿结局：与FXR/FGF15信号传导相关

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-22 DOI: 10.4014/jmb.2504.04017

Le Huang, Hua Li, Weitao Yang, Lihui Huang, Qiuling Chen, Shengnan Li, Zhi Zou, Lijing Zhao, Zhihua Zeng

{"title":"L-Theanine Ameliorates Metabolic Dysregulation and Adverse Fetal Outcomes in a Mice Model of Gestational Obesity: Association with FXR/FGF15 Signaling.","authors":"Le Huang, Hua Li, Weitao Yang, Lihui Huang, Qiuling Chen, Shengnan Li, Zhi Zou, Lijing Zhao, Zhihua Zeng","doi":"10.4014/jmb.2504.04017","DOIUrl":"https://doi.org/10.4014/jmb.2504.04017","url":null,"abstract":"In this study, we investigated whether L-theanine (LTA) ameliorates adverse pregnancy outcomes in high-fat diet (HFD)-induced gestational obesity mice. Gestational obese mice models received HFD and fecal microbiota transplantation (FMT) from pregnant obese women, followed by LTA treatment. Gut microbiota DNA from six obese and six normal pregnant women was analyzed. Also assessed were lipid profiles, inflammatory factors, gut permeability, FXR/FGF15 expression, pup weight, and placental function. Alpha- and beta-diversity analyses showed reduced gut microbial diversity in the obese pregnant women. Postpartum hemorrhage, cholesterol, and triglycerides inversely correlated with Weissella, while BMI was positively associated with Escherichia-Shigella. Neonatal weight correlated positively with Subdoligranulum and negatively with Megamonas. Fasting glucose was significantly positively associated with Bacteroides vulgatus, whereas neonatal body weight inversely correlated with Eubacterium ramulus. In gestational obesity mice, LTA administration reduced weight gain, visceral/gonadal adiposity, metabolic markers (fasting glucose/insulin/cholesterol), gut barrier dysfunction (TNF-α, IL-6, IL-8, Claudin-2), and linked to FXR/FGF15 pathway alterations. Furthermore, LTA intervention suppressed MCP-1, IL-1β, F4/80 and hepatic lipid metabolism regulators (CD36, SREBP1c, SCD1, GLUT4, Cyp7a1, IRS-1), while also mitigating placental tissue junction zone abnormalities and pup weight. To sum up, LTA-mediated attenuation of adverse pregnancy outcomes associates with FXR/FGF15 pathway alterations, concomitant with restoration of metabolic homeostasis and inflammation suppression.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504017"},"PeriodicalIF":3.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Multiplex TaqMan Real-Time RT-PCR Assay for the Rapid Differential Detection of Classic, MLB-Clade and VA-Clade Human Astroviruses. 建立多重TaqMan实时RT-PCR快速鉴别检测经典、mlb支和va支人类星状病毒的方法。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-22 DOI: 10.4014/jmb.2506.06008

Peipei Zheng, Jianhang Wang, Hongwei Yu, Qingping Luo, Huijie Gao, Zengjun Jin, Yilei Shi, Hanzhong Wang, Penghui Li, Feng Tian

{"title":"Development of a Multiplex TaqMan Real-Time RT-PCR Assay for the Rapid Differential Detection of Classic, MLB-Clade and VA-Clade Human Astroviruses.","authors":"Peipei Zheng, Jianhang Wang, Hongwei Yu, Qingping Luo, Huijie Gao, Zengjun Jin, Yilei Shi, Hanzhong Wang, Penghui Li, Feng Tian","doi":"10.4014/jmb.2506.06008","DOIUrl":"https://doi.org/10.4014/jmb.2506.06008","url":null,"abstract":"The classical human astrovirus (HAstV) causes acute or severe gastroenteritis mainly among children and the elderly populations. The novel divergent HAstV-VA and HAstV-MLB clades related to encephalitis in immunocompromised patients, are genetically more similar to certain animal astroviruses, and have the potential for cross-species transmission. To identify all the three HAstV clades in a single reaction, we developed a quantitative reverse transcription PCR (RT-qPCR) method. Primers and probes were designed based on the conserved regions of the HAstV genomes that exhibited inter-clade divergence. This method was highly sensitive, as the detection limits were 95, 12.5 and 25 copies/μl for the classical HAstV, HAstV-MLB and HAstV-VA strains, respectively. Intra- and inter-assay variability revealed excellent reproducibility. Furthermore, this multiplex assay showed no cross-reactivity with other human pathogens. A total of 326 anal swabs from outpatients with AGE were examined: 5.83% were positive for the classical HAstV, 1.53% for the HAstV-MLB, and 0.31% for the HAstV-VA, while 0.31% for co-infections of HAstV-MLB/HAstV-VA. Conclusively, the developed multiplex RT-qPCR assay represents a tool with potential for laboratory and clinical diagnoses, epidemiological surveillance, prevention and control of the HAstV infection.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506008"},"PeriodicalIF":3.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TLR4/NF-κB-Mediated Anti-Inflammatory and Cognitive Protective Actions of Curcuma longa and Ar-turmerone in Alzheimer's Disease Models. 姜黄和Ar-turmerone在阿尔茨海默病模型中TLR4/NF-κ b介导的抗炎和认知保护作用

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-17 DOI: 10.4014/jmb.2506.06044

Kyo-Nyeo Oh, Donghyuck Bae, Dool-Ri Oh, Ji-Ae Hong, Yujin Kim, Eun Kim, Seon Ah Son, Kwang Youl Lee, Sunoh Kim

{"title":"TLR4/NF-κB-Mediated Anti-Inflammatory and Cognitive Protective Actions of Curcuma longa and Ar-turmerone in Alzheimer's Disease Models.","authors":"Kyo-Nyeo Oh, Donghyuck Bae, Dool-Ri Oh, Ji-Ae Hong, Yujin Kim, Eun Kim, Seon Ah Son, Kwang Youl Lee, Sunoh Kim","doi":"10.4014/jmb.2506.06044","DOIUrl":"10.4014/jmb.2506.06044","url":null,"abstract":"In our previous study, we systematically compared various extraction methods and identified the 80% ethanolic extract of Curcuma longa L. (CL-80) as the most effective in protecting neurons from stress-induced damage in both in vitro and in vivo models. Although curcumin, a major constituent of C. longa, has demonstrated neuroprotective effects, the role of ar-turmerone, a bioactive sesquiterpenoid also derived from C. longa, remains underexplored in Alzheimer's disease (AD) models. In this study, we evaluated the anti-inflammatory and cognitive-protective effects of CL-80 and ar-turmerone in both in vitro and in vivo models of amyloid-β (Aβ)-induced neurotoxicity. Primary cultured rat hippocampal neurons exposed to Aβ25-35 showed significantly increased expression of TNF-α, IFN-β, and iNOS, all of which were dose-dependently attenuated by CL-80 or ar-turmerone. Furthermore, both compounds suppressed Aβ-induced activation of the TLR4/NF-κB signaling axis at mRNA and protein levels. In an Aβ1-42-injected mouse model, oral administration of CL-80 or ar-turmerone significantly improved learning and memory performance in the Morris water maze and passive avoidance tests. Biochemical analyses of hippocampal tissues revealed reduced TLR4 expression and NF-κB activation, decreased acetylcholinesterase (AChE) activity, and restoration of acetylcholine (ACh) levels following treatment. Collectively, these findings suggest that both CL-80 and ar-turmerone exert neuroprotective effects by inhibiting TLR4/NF-κB-mediated neuroinflammation and preserving cholinergic function in an AD animal model. This study offers novel insight into the therapeutic potential of C. longa constituents for the treatment of AD.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506044"},"PeriodicalIF":3.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Anaerobic Biodegradation and Biomethane Production from Bioplastics by the Addition of Aerobically Prepared Triacylglycerol Lipase. 添加好氧制备的三酰甘油脂肪酶促进生物塑料的厌氧生物降解和生物甲烷生产。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-17 DOI: 10.4014/jmb.2504.04047

Jinok Oh, Jeong Hyeon Hwang, Yebin Han, Gaeun Lim, Sang Ho Lee, Jae-Seok Kim, Shashi Kant Bhatia, Yung-Hun Yang

{"title":"Enhanced Anaerobic Biodegradation and Biomethane Production from Bioplastics by the Addition of Aerobically Prepared Triacylglycerol Lipase.","authors":"Jinok Oh, Jeong Hyeon Hwang, Yebin Han, Gaeun Lim, Sang Ho Lee, Jae-Seok Kim, Shashi Kant Bhatia, Yung-Hun Yang","doi":"10.4014/jmb.2504.04047","DOIUrl":"10.4014/jmb.2504.04047","url":null,"abstract":"This study aimed to overcome the limited biodegradability of bioplastics under anaerobic conditions. With polycaprolactone (PCL) as a model system, the effect of a bioplastic-degrading enzyme, triacylglycerol lipase (TGL), on its degradation and biomethane production was investigated. As the PCL film did not show evidence of any degradation over 14 days under anaerobic conditions in the sludge, TGL from Bacillus sp. JY35 was added to promote PCL breakdown into its monomeric form, which could be used for methane production. Application of 200 units/mg of TGL in the sludge led to a 33% increase in PCL degradation over 7 days, with sustained lipase activity despite the decreasing trend in effectiveness after 72 h. Across all type of samples, methane production in the TGL-supplemented sludge increased 1.8-fold across sludge types and up to 2.2-fold when bioplastics other than PCL underwent degradation, compared with that in the untreated sludge. Our result showed the addition of concentrated enzyme could effectively improve bioplastics biodegradability concomitant with methane production under anaerobic conditions, thus offering a feasible approach for optimizing anaerobic degradation with various bioplastics such as Polybuthylene succinate (PBS), and Polybutylene adipate-co-terephthalate (PBAT) although it will take longer time than PCL.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504047"},"PeriodicalIF":3.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Recombinant S1 Protein of Porcine Epidemic Diarrhea Virus in Recombinant CHO Cells for Application in Indirect ELISA. 猪流行性腹泻病毒在重组CHO细胞中重组S1蛋白的产生及其间接酶联免疫吸附测定

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-17 DOI: 10.4014/jmb.2506.06050

Eun-Ji Lee, Sungkyun Kim, Tae-Ho Kim, Na-Yeong Heo, Hyun-Seung Kim, So Hui Ryu, Seung Jin Koo, Hokeun Won, Yeon-Gu Kim

{"title":"Production of Recombinant S1 Protein of Porcine Epidemic Diarrhea Virus in Recombinant CHO Cells for Application in Indirect ELISA.","authors":"Eun-Ji Lee, Sungkyun Kim, Tae-Ho Kim, Na-Yeong Heo, Hyun-Seung Kim, So Hui Ryu, Seung Jin Koo, Hokeun Won, Yeon-Gu Kim","doi":"10.4014/jmb.2506.06050","DOIUrl":"10.4014/jmb.2506.06050","url":null,"abstract":"The N-terminal S1 subunit of the spike protein (S1 protein) of porcine epidemic diarrhea virus (PEDV) is recognized as a potential diagnostic antigen for detecting anti-PEDV immunoglobulin A (IgA) levels in colostrum, a key indicator for assessing passive immunity against PEDV infection. Given the advantages of producing Fc-fusion proteins in mammalian cells, it is important to investigate how the addition of the Fc region affects the diagnostic performance of the PEDV-S1 protein. In this study, we successfully produced full-length PEDV-S1 protein fused with an Fc region (PEDV-S1-Fc protein) via transient gene expression in recombinant Chinese hamster ovary (rCHO) cells adapted to serum-free suspension culture. The resulting expression showed significantly higher volumetric productivity than previously reported. The aglycosylated form of PEDV-S1-Fc protein was also generated using PNGase F treatment of the purified protein, as well as tunicamycin treatment to inhibit glycosylation during cell culture. Notably, neither the addition of the Fc region nor the removal of N-glycans markedly affected the diagnostic function, as demonstrated by indirect ELISA using PEDV-positive and PEDV-negative colostrum samples. Moreover, the diagnostic performance of the PEDV-S1-Fc protein was further validated using total IgA purified from PEDV-positive colostrum. In summary, the full-length Fc-fused PEDV-S1 protein produced in rCHO cell culture exhibited high productivity and purity, making it a promising antigenic candidate for indirect ELISA-based detection of anti-PEDV IgA in colostrum.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506050"},"PeriodicalIF":3.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer Activity of Benzomalvin Derivatives Isolated from Penicillium spathulatum SF7354, a Symbiotic Fungus from Azorella monantha. 鸡血藤青霉菌SF7354苯佐马文衍生物的抗癌活性研究

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-16 DOI: 10.4014/jmb.2505.05003

Min Seo Jeon, Ju-Mi Hong, Jaewon Kim, Jina Kim, Sojin Kim, Jae Hak Sohn, Se Jong Han, Joung Han Yim, Il-Chan Kim

{"title":"Anticancer Activity of Benzomalvin Derivatives Isolated from Penicillium spathulatum SF7354, a Symbiotic Fungus from Azorella monantha.","authors":"Min Seo Jeon, Ju-Mi Hong, Jaewon Kim, Jina Kim, Sojin Kim, Jae Hak Sohn, Se Jong Han, Joung Han Yim, Il-Chan Kim","doi":"10.4014/jmb.2505.05003","DOIUrl":"10.4014/jmb.2505.05003","url":null,"abstract":"Penicillium spathulatum SF7354 was isolated from the extremophilic plant Azorella monantha collected in Chilean Patagonia and investigated for its anticancer potential. Crude extracts of SF7354 exhibited significant cytotoxic activity against multiple human cancer cell lines, with the most pronounced effect observed in HCT116 cells. Flow cytometry analysis revealed that treatment with the extract induced time-dependent apoptosis and sub-G1 accumulation, indicating activation of programmed cell death. Cell cycle analysis further showed early G0/G1 arrest, followed by a progressive increase in apoptotic populations. Western blot analysis demonstrated notable alterations in PARP and p53 protein levels, suggesting a p53-dependent mechanism of apoptosis. HPLC-based purification of the extract led to the isolation of five benzomalvin derivatives (A-E), all of which exhibited dose- and time-dependent cytotoxicity. These findings suggest that SF7354-derived benzomalvins act through apoptosis-associated mechanisms and represent promising candidates for the development of novel anticancer agents.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505003"},"PeriodicalIF":3.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated and Programmable Cell-Free Systems for Scalable Synthetic Biology with a Focus on Biofoundry Integration. 自动化和可编程的无细胞系统，可扩展的合成生物学与生物铸造厂集成的重点。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-09-16 DOI: 10.4014/jmb.2507.07019

Ji-Su Jun, Sujin Hong, Jun-Hong Park, Jonghyeok Shin, Dae-Hee Lee

{"title":"Automated and Programmable Cell-Free Systems for Scalable Synthetic Biology with a Focus on Biofoundry Integration.","authors":"Ji-Su Jun, Sujin Hong, Jun-Hong Park, Jonghyeok Shin, Dae-Hee Lee","doi":"10.4014/jmb.2507.07019","DOIUrl":"10.4014/jmb.2507.07019","url":null,"abstract":"Cell-free protein synthesis (CFPS) has been used as a transformative technology in synthetic biology, providing a programmable, scalable, and automation-compatible platform for biological engineering. Freed from the limitations of cell viability and growth, CFPS enables rapid design iteration, precise control of reaction conditions, and high-throughput experimentation. Recent integration of CFPS with biofoundries-automated, high-throughput biological engineering platforms-has dramatically accelerated the Design-Build-Test-Learn cycle, facilitating applications such as enzyme engineering, metabolic pathway prototyping, biosensor development, and remote biomanufacturing. Advances in automation technologies, including liquid-handling robotics and digital microfluidics, have further enhanced the scalability and reproducibility of CFPS workflows. Additionally, coupling CFPS with machine learning has enabled predictive optimization of genetic constructs and biosynthetic systems. This review highlights the technological innovations driving the convergence of CFPS and automated biofoundries, outlining current capabilities, challenges, and future directions toward programmable, scalable, and distributed biological engineering.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507019"},"PeriodicalIF":3.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0