Journal of microbiology and biotechnology最新文献_第2页

Aspergillus fumigatus from Pathogenic Fungus to Unexplored Natural Treasure: Changing the Concept.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-11 DOI: 10.4014/jmb.2411.11082

Sabrin R M Ibrahim, Hagar Mohamed Mohamed, Anfal S Aljahdali, Samar S A Murshid, Shaimaa G A Mohamed, Hossam M Abdallah, Gamal A Mohamed

{"title":"Aspergillus fumigatus from Pathogenic Fungus to Unexplored Natural Treasure: Changing the Concept.","authors":"Sabrin R M Ibrahim, Hagar Mohamed Mohamed, Anfal S Aljahdali, Samar S A Murshid, Shaimaa G A Mohamed, Hossam M Abdallah, Gamal A Mohamed","doi":"10.4014/jmb.2411.11082","DOIUrl":"10.4014/jmb.2411.11082","url":null,"abstract":"The Aspergillus genus is one of the oldest known fungal genera. Its species can survive in extremely difficult environmental conditions and can affect plants, animals, and human health due to their pathogenic characteristics. Also, they represent an enormous chemical and biological repertoire. A. fumigatus is a fungal pathogen that has been thoroughly investigated due to its medical significance. Additionally, A. fumigatus has revealed a capacity to biosynthesize different classes of secondary metabolites such as alkaloids, terpenes, sterols, quinones, peptides, and benzophenones. Alkaloids are the most common type of secondary metabolites that A. fumigatus produces, and they have a variety of biological functions. This current study aimed to highlight the positive aspects of this fungus. The published research on the structures, biological functions, and biosynthesis of A. fumigatus's isolated metabolites were emphasized in this work. The current review comprised more than 400 compounds, with more than 145 references that were published between 1965 and August 2024. This review underscores the role of this fungus as a reservoir of bioactive compounds with antimicrobial, anticancer, anti-inflammatory, and agricultural applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411082"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lacticaseibacillus paracasei HP7 Improves Gastric Emptying by Modulating Digestive Factors in a Loperamide-Induced Functional Dyspepsia Mouse Model.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-11 DOI: 10.4014/jmb.2412.12035

Ji-Woong Jeong, Daehyeop Lee, Hyeonji Kim, Hyeonjun Gwon, Kippeum Lee, Joo-Yun Kim, Jae-Jung Shim, Jae-Hwan Lee

{"title":"Lacticaseibacillus paracasei HP7 Improves Gastric Emptying by Modulating Digestive Factors in a Loperamide-Induced Functional Dyspepsia Mouse Model.","authors":"Ji-Woong Jeong, Daehyeop Lee, Hyeonji Kim, Hyeonjun Gwon, Kippeum Lee, Joo-Yun Kim, Jae-Jung Shim, Jae-Hwan Lee","doi":"10.4014/jmb.2412.12035","DOIUrl":"10.4014/jmb.2412.12035","url":null,"abstract":"Functional dyspepsia (FD) is a condition characterized by persistent indigestion symptoms without a clear underlying cause. We investigated the effects on FD of Lacticaseibacillus paracasei HP7 (HP7), which was isolated from kimchi and is known to inhibit Helicobacter pylori. In a mouse model of loperamide-induced FD, HP7 administration significantly improved gastrointestinal (GI) motility and gastric emptying, as demonstrated by increased charcoal movement in the GI tract, decreased stomach weight, and the amount of remaining phenol red solution. HP7 administration significantly enhanced peristalsis by upregulating the expression of smooth muscle contraction-related genes, such as the 5HT4 receptor, anoctamin-1, ryanodine receptor 3, and smooth muscle myosin light-chain kinase. In addition, digestive factors, including GI regulatory hormones such as gastrin, gastric inhibitory peptide, and peptide YY, and the activity of digestive enzymes, such as amylase, trypsin, and lipase, were restored to normal levels. These results indicate that HP7 is a promising probiotic strain to alleviate FD symptoms by modulating peristalsis and digestive factors.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412035"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fecal Microbiome and Urine Metabolome Profiling of Type 2 Diabetes.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-11 DOI: 10.4014/jmb.2411.11071

Hye-Min Yi, Seok Won, Juhan Pak, Seong-Eun Park, Mi-Ri Kim, Ji-Hyun Kim, Eun-Young Park, Sun-Young Hwang, Mee-Hyun Lee, Hong-Seok Son, Suryang Kwak

{"title":"Fecal Microbiome and Urine Metabolome Profiling of Type 2 Diabetes.","authors":"Hye-Min Yi, Seok Won, Juhan Pak, Seong-Eun Park, Mi-Ri Kim, Ji-Hyun Kim, Eun-Young Park, Sun-Young Hwang, Mee-Hyun Lee, Hong-Seok Son, Suryang Kwak","doi":"10.4014/jmb.2411.11071","DOIUrl":"https://doi.org/10.4014/jmb.2411.11071","url":null,"abstract":"Type 2 diabetes is a prevalent metabolic disorder with serious health consequences, necessitating both enhanced diagnostic methodologies and comprehensive elucidation of its pathophysiological mechanisms. We compared fecal microbiome and urine metabolome profiles in type 2 diabetes patients versus healthy controls to evaluate their respective diagnostic potential. Using a cohort of 94 subjects (48 diabetics, 46 controls), this study employed 16S rRNA amplicon sequencing for fecal microbiome analysis and GC-MS for urinary metabolomics. While fecal microbiome alpha diversity showed no significant differences between groups, urinary metabolomics demonstrated distinct structural patterns and higher evenness in type 2 diabetes patients. The study identified several diabetes-associated urinary metabolites, including elevated levels of glucose and inositol, along with decreased levels of 6 urine metabolites including glycolic acid, hippurate, and 2-aminoethanol. In the fecal microbiome, genera such as Escherichia-Shigella showed positive correlation with type 2 diabetes, while Lacticaseibacillus demonstrated negative correlation. Receiver operating characteristic curve analyses revealed that urinary metabolites exhibited superior diagnostic potential compared to fecal microbiome features, with an area under the curve of 0.90 for the combined metabolite model versus 0.82 for the integrated bacterial taxa model. These findings suggest that urinary metabolomics may offer a more reliable approach for type 2 diabetes diagnosis compared to fecal 16S metabarcoding, while highlighting the potential of multi-marker panels for enhanced diagnostic accuracy.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411071"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Live Biotherapeutic Products for Metabolic Diseases: Development Strategies, Challenges, and Future Directions.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-11 DOI: 10.4014/jmb.2410.10054

Heonhae Min, Kyu-Sung Choi, Saebom Yun, Sungho Jang

{"title":"Live Biotherapeutic Products for Metabolic Diseases: Development Strategies, Challenges, and Future Directions.","authors":"Heonhae Min, Kyu-Sung Choi, Saebom Yun, Sungho Jang","doi":"10.4014/jmb.2410.10054","DOIUrl":"10.4014/jmb.2410.10054","url":null,"abstract":"Metabolic diseases, such as obesity, type 2 diabetes, and non-alcoholic fatty liver disease, have emerged as major global health challenges. Recent research has revealed that the gut microbiome is closely associated with the development of these conditions. The Food and Drug Administration has recognized certain probiotic strains with therapeutic potential, classifying them as live biotherapeutic products (LBPs). LBPs, which are derived from naturally occurring microorganisms, may present an effective strategy for treating metabolic diseases by restoring gut microbiota balance and regulating metabolic functions. This review explores the development of LBPs specifically for metabolic disease treatments, covering every phase from strain identification, non-clinical and clinical trials, manufacturing and formulation to regulatory approval. Furthermore, it addresses the challenges involved in the commercialization of these therapies. By offering critical insights into the research and development of LBPs for metabolic disease treatment, this review aims to contribute to the progress of these promising therapies.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2410054"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactococcus lactis as an Effective Mucosal Vaccination Carrier: a Systematic Literature Review.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-07 DOI: 10.4014/jmb.2411.11036

Suryanata Kesuma, Tri Yudani Mardining Raras, Sri Winarsih, Takeshi Shimosato, Valentina Yurina

{"title":"Lactococcus lactis as an Effective Mucosal Vaccination Carrier: a Systematic Literature Review.","authors":"Suryanata Kesuma, Tri Yudani Mardining Raras, Sri Winarsih, Takeshi Shimosato, Valentina Yurina","doi":"10.4014/jmb.2411.11036","DOIUrl":"10.4014/jmb.2411.11036","url":null,"abstract":"Lactococcus lactis has potential as a mucosal vaccine delivery system. L. lactis can express antigens from bacteria or viruses, which are tightly controlled using nisin. Although L. lactis-based vaccine shows great promise, no product is ready for human use. Several studies have been conducted to develop L. lactis-based vaccine, and the efficacy of these vaccines has been evaluated in many scientific articles. This paper aims to review key aspects of current knowledge on the promising characteristics of L. lactis and to suggest its implications for vaccine design. Articles were obtained online using inclusion and exclusion criteria through Harzing's Publish or Perish. The article assessment used the Joanna Briggs Institute critical appraisal checklist for quasi-experimental studies. The efficacy evaluation of 24 articles showed that L. lactis-based vaccine can induce IgA and IgG as humoral immune responses; T CD4, T CD8, and B cells as cellular immune responses; and various proinflammatory cytokines such as IFN-γ, TNF-α, IL-2, IL-4, IL-8, IL-10, IL-12, IL-17. L. lactis is suitable as a vector carrier for oral or nasal mucosal vaccines targeting bacterial and viral infections. The development of L. lactis as a vaccine delivery system is promising.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411036"},"PeriodicalIF":2.5,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Sero-Specific-Gene Dependent Multiplex PCR Enhances the Discrimination of Major Listeria monocytogenes Serovars.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-07 DOI: 10.4014/jmb.2411.11081

Jing Wang, Jingliang Qin, Bin Hu, Zixu Zhang, Boyang Cao, Xi Guo

{"title":"A Novel Sero-Specific-Gene Dependent Multiplex PCR Enhances the Discrimination of Major Listeria monocytogenes Serovars.","authors":"Jing Wang, Jingliang Qin, Bin Hu, Zixu Zhang, Boyang Cao, Xi Guo","doi":"10.4014/jmb.2411.11081","DOIUrl":"10.4014/jmb.2411.11081","url":null,"abstract":"Listeria monocytogenes is a foodborne bacterial pathogen distributed worldwide. Serotyping is extensively applied in the classification of L. monocytogenes and is crucial in the early stage of epidemiological tracing. Among the 13 serovars, 1/2a, 1/2b, 1/2c, and 4b are the ones most frequently isolated. Numerous PCR-based methods have been presented, however, their target genes, lmo0737, ORF2110 and ORF2819, are prone to horizontal transfer or loss in certain strains, thus leading to incorrect serovar designation. Herein, we selected novel sero-specific genes and developed an improved multiplex PCR assay. The specificity of our assay was confirmed by the use of target and nontarget Listeria reference strains, as well as by the use of isolates yielding incorrect profiles in previous studies. Sensitivity tests indicated that a minimum of 5 ng of genomic DNA or approximately 3 × 106 CFU of pure culture could be detected. Many collected isolates and genomes of global isolates were used to evaluate the specificity and reproducibility of our assay. The agreement between our assay and the agglutination test was 95%, and the one between our assay and the Doumith scheme was 97%. However, our assay overcomes the drawbacks of currently used PCR-based approaches by exhibiting 100% accuracy for certain strains and clones, for instance, ST782 within the hypervirulent CC2 and ST218 that were incorrectly assigned by the Doumith scheme. In conclusion, the developed assay herein will be a powerful tool and an alternative for the classification of L. monocytogenes strains in foodborne outbreak investigations and surveillance programs.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411081"},"PeriodicalIF":2.5,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Inhibitory Effects of Cordyceps militaris ARA301 Extract on Lipopolysaccharide-Induced Lung Injury in vivo.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-03-06 DOI: 10.4014/jmb.2412.12043

Hye Kang Seong, Min Jeong Kim, Ardina Nur Fauziah, Hyeon Su Jeong, Hyo Jeong Kim, Chung Yeol Yang, Su Jin Park, Soo Yeon Bae, Sung Keun Jung

{"title":"The Inhibitory Effects of Cordyceps militaris ARA301 Extract on Lipopolysaccharide-Induced Lung Injury in vivo.","authors":"Hye Kang Seong, Min Jeong Kim, Ardina Nur Fauziah, Hyeon Su Jeong, Hyo Jeong Kim, Chung Yeol Yang, Su Jin Park, Soo Yeon Bae, Sung Keun Jung","doi":"10.4014/jmb.2412.12043","DOIUrl":"10.4014/jmb.2412.12043","url":null,"abstract":"Lung injury is a critical health concern associated with severe inflammatory responses and tissue damage that can weaken respiratory function and potentially become life-threatening in severe cases. This study aimed to establish a mouse model of lung injury induced by lipopolysaccharides (LPS) derived from Klebsiella pneumoniae and to evaluate whether Cordyceps militaris ARA301 extract (CME) can prevent lung injury. CME was orally administered to mice for three consecutive days, followed by intranasal LPS administration. Mice were sacrificed 24 h later to analyze immune cell alterations and inflammatory responses through bronchoalveolar lavage fluid (BALF) and tissue analyses. CME administration inhibited immune cell infiltration, tissue fibrosis, and excessive mucus deposition induced by intranasal LPS administration. Furthermore, CME suppressed the expression of mucin 5AC (MUC5AC), a protein involved in mucus production, as well as the expression of inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in lung tissues. In BALF, CME reduced the production of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and C-X-C motif chemokine ligand 1 (CXCL1), which were elevated due to LPS administration. Additionally, CME decreased the total immune cell, neutrophil, monocyte, and eosinophil numbers in BALF. The anti-inflammatory activity of CME was evaluated in vitro using RAW 264.7 cells. CME treatment reduced the secretion of pro-inflammatory cytokines induced by LPS and inhibited the phosphorylation of p65, inhibitor of kappa B alpha (IκBα), and IκB kinase alpha (IKKα). These findings suggest that CME has potential as a functional health supplement effective in preventing lung injury.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412043"},"PeriodicalIF":2.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: Effect of Light Regime on Candidatus Puniceispirillum marinum IMCC1322 in Nutrient-Replete Conditions.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-02-28 DOI: 10.4014/jmb.2025.3503.C01

Hyun-Myung Oh, Ji Hyen Lee, Ahyoung Choi, Sung-Hyun Yang, Gyung-Hoon Shin, Sung Gyun Kang, Jang-Cheon Cho, Hak Jun Kim, Kae-Kyoung Kwon

引用次数: 0

Heat-Killed Leuconostoc mesenteroides H40 Alleviates Cognitive Impairment by Anti-Inflammation and Antioxidant Effects in a Scopolamine-Induced Mouse Model.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-02-25 DOI: 10.4014/jmb.2411.11013

Na-Kyoung Lee, Yunjung Lee, Jiyoung Hwang, Eunju Park, Hyun-Dong Paik

{"title":"Heat-Killed Leuconostoc mesenteroides H40 Alleviates Cognitive Impairment by Anti-Inflammation and Antioxidant Effects in a Scopolamine-Induced Mouse Model.","authors":"Na-Kyoung Lee, Yunjung Lee, Jiyoung Hwang, Eunju Park, Hyun-Dong Paik","doi":"10.4014/jmb.2411.11013","DOIUrl":"10.4014/jmb.2411.11013","url":null,"abstract":"Leuconostoc mesenteroides H40 (H40), originally isolated from kimchi, has been shown to exhibit probiotic characteristics and a neuroprotective effect in SH-SY5Y cells. In this study, we investigated the modulatory effects of heat-killed H40 (H-H40) in a scopolamine-induced (1 mg/kg/day) mouse model of cognitive impairment. H-H40 at either 1 × 108 or 1 × 109 CFU/day alleviated scopolamine-induced cognitive impairment in the novel object recognition and Y-maze tests. Neuroinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, inducible NO synthase (iNOS), and cyclooxygenase (COX)-2 were all found to be decreased by H-H40 treatment. Moreover, changes in neurotransmitter levels and synaptic plasticity were further confirmed through measurement of acetylcholinesterase, acetylcholine, choline acetyltransferase, and brain-derived neurotrophic factor (BDNF) levels. H-H40 increased β-secretase levels, but decreased amyloid-β levels. In addition, the antioxidant effects of catalase and GPx were demonstrated. Overall, our results showed that H-H40 exerts positive cognitive effects by anti-inflammatory and antioxidant activities in a mouse model of scopolamine-induced cognitive impairment. H-H40 could be used as a prophylactic functional food for improving cognition.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411013"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amelioration of Astrocytic Dysfunction via AQP4/LRP1 Pathway by Zizania latifolia and Tricin in C6 Cells Exposed to Amyloid β and High-Dose Insulin and in Mice Treated with Scopolamine.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-02-25 DOI: 10.4014/jmb.2412.12026

Seun-Ah Yang, Se-Ho Park, Eun-Hye Kim, Won-Bin Bae, Kwang-Hwan Jhee

{"title":"Amelioration of Astrocytic Dysfunction via AQP4/LRP1 Pathway by Zizania latifolia and Tricin in C6 Cells Exposed to Amyloid β and High-Dose Insulin and in Mice Treated with Scopolamine.","authors":"Seun-Ah Yang, Se-Ho Park, Eun-Hye Kim, Won-Bin Bae, Kwang-Hwan Jhee","doi":"10.4014/jmb.2412.12026","DOIUrl":"10.4014/jmb.2412.12026","url":null,"abstract":"Zizania latifolia and its bioactive compound tricin have been recognized for their anti-inflammatory, anti-allergic, and anti-aging properties. However, the impact of Z. latifolia extract (ZLE) and tricin on astrocyte dysfunction, particularly related to disruptions in the amyloid β (Aβ) clearance pathway, has not been extensively studied. This research aims to explore the regulatory effects of ZLE and tricin on astroglial dysfunction, utilizing astrocytic differentiated C6 cells (passages 75~85) subjected to Aβ and high-dose insulin, as well as scopolamine-induced mice. Results revealed that ZLE (500 μg/ml) and tricin (1 μg/ml) significantly upregulated the expression of astrocyte proteins GFAP and AQP4, brain-derived neurotrophic factor (BDNF), low-density lipoprotein receptor-related protein 1 (LRP1), and matrix metalloproteinases (MMPs) in C6 cells treated with Aβ and high-dose insulin. Furthermore, oral administration of ZLE (100 and 300 mg/kg) and tricin (0.3 mg/kg) in mice led to an increase in acetylcholine (ACh) levels and upregulation of insulin-degrading enzyme (IDE), LRP1, and MMPs, while reducing the levels of acetylcholinesterase (AChE), Aβ and ApoE4. These findings suggest that ZLE and tricin may ameliorate Aβ and high-dose insulin-induced astrocyte dysfunction in C6 cells and scopolamine-treated mice, potentially through the AQP4/LRP1 pathway.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412026"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0