Journal of microbiology and biotechnology最新文献

{"title":"Contribution of MylA in Conidiogenesis, Conidia Germination, and beta-Glucan Biosynthesis in <i>Aspergillus fumigatus</i>.","authors":"Ye-Eun Son, He-Jin Cho, Jae-Hyuk Yu, Hee-Soo Park","doi":"10.4014/jmb.2607.07015","DOIUrl":"10.4014/jmb.2607.07015","url":null,"abstract":"<p><p>The conidia of <i>Aspergillus fumigatus</i> are the primary infectious propagules responsible for initiating invasive aspergillosis, and their development and physiology are closely regulated by numerous transcription factors. Our previous study demonstrated that the MYB-like transcription factor MylA plays a critical role in asexual sporulation and conidial viability in <i>Aspergillus nidulans</i>. However, the function of MylA in <i>A. fumigatus</i> has not yet been investigated. This study aimed to investigate the role of MylA, a member of the MYB-like transcription factor family, in conidiogenesis, conidial viability, cell wall integrity, and germination in <i>A. fumigatus</i>. Deletion of <i>mylA</i> resulted in reduced vegetative growth, abnormal conidiophore development, and a significant decrease in conidial production. Furthermore, Δ<i>mylA</i> conidia exhibited reduced trehalose accumulation, decreased viability, and increased sensitivity to heat and oxidative stress. Deletion of <i>mylA</i> also delayed conidial germination and impaired biofilm formation. Transcriptomic analysis of conidia identified approximately 2,200 differentially expressed genes, including those involved in β-glucan biosynthesis. Notably, deletion of <i>mylA</i> significantly upregulated the expression of <i>fks1</i> and <i>gel1</i>, resulting in increased β-glucan accumulation in the conidial cell wall. Collectively, these findings demonstrate that MylA is a key regulator of conidiogenesis, conidial viability, germination, and β-glucan biosynthesis in <i>A. fumigatus</i>.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2607015"},"PeriodicalIF":3.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ribes fasciculatum</i> Leaf Water Extract Enhances Macrophage Immune Responses via TLR4-Mediated JNK and NF-κB Activation in RAW264.7 Cells.","authors":"So Jung Park, Jeong Won Choi, Hyeok Jin Choi, Gyeong Eun Im, Bo Hee Seo, Ha Eun Kim, Kwan Been Park, Seon A Kim, Jenna Jung, Myung Suk Choi, Jin Boo Jeong","doi":"10.4014/jmb.2607.07022","DOIUrl":"10.4014/jmb.2607.07022","url":null,"abstract":"<p><p>This study investigated the immunostimulatory activity and underlying mechanism of <i>Ribes fasciculatum</i> leaf water extract in RAW264.7 macrophages. Among extracts prepared using water or ethanol-containing solvents, only the water extract induced NO production. Further evaluation of extraction temperature and time identified the extract prepared at 20°C for 24 h (RFL-DW20) as the most active sample under the tested conditions, with NO-inducing activity increasing after 12 h of extraction and reaching a plateau by 24 h. RFL-DW20 increased NO and PGE2 production, upregulated iNOS and COX-2 expression, and enhanced IL-6 and TNF-α production without cytotoxicity. RFL-DW20 also increased IL-1β mRNA expression but did not significantly alter extracellular IL-1β levels. RFL-DW20 also increased neutral red uptake, indicating enhanced macrophage functional activity. The immunostimulatory effects of RFL-DW20 were strongly inhibited by TAK-242, SP600125, and BAY 11-7082, suggesting the involvement of TLR4, JNK, and NF-κB signaling. RFL-DW20 activated MAPK and NF-κB signaling, including rapid IκBα phosphorylation/degradation and p65 phosphorylation, while pharmacological inhibition identified JNK and NF-κB as major functional mediators of the NO response. TAK-242 markedly suppressed RFL-DW20-induced JNK and p65 phosphorylation. Ethanol precipitation further enriched its NO-inducing activity. These findings suggest that RFL-DW20 activates macrophages through the TLR4/JNK/NF-κB signaling axis and may serve as a natural immune-stimulating material.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2607022"},"PeriodicalIF":3.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Hydrogel-to-Oleogel Ratio and Potato Protein Isolate Concentration on the 3D Printing and Post-Heating Properties of Food-Grade Bigels.","authors":"Seri Park, Jiho Sohn, Yourim Oh, Jin-Kyu Rhee","doi":"10.4014/jmb.2607.07038","DOIUrl":"10.4014/jmb.2607.07038","url":null,"abstract":"<p><p>This study investigated the effects of the hydrogel-to-oleogel ratio and potato protein isolate (PPI) concentration on the printing and post-heating properties of food-grade bigels. Bigels with hydrogel-to-oleogel ratios of 10:0, 7:3, 5:5, 3:7, and 0:10 were evaluated for physical stability, rheological behavior, structural recovery, and 3D printing performance. Among the biphasic formulations, H₇O₃ exhibited the highest liquid-binding capacity (99.43%) and structural recovery ratio (88.7%) and produced lateral dimensions closest to those of the CAD model. H₇O₃ was therefore selected for PPI incorporation at 0-10 wt%. PPI incorporation improved the initial dimensional fidelity of the printed constructs and reduced rheological softening during heating. After heating at 120°C for 10 min, the 0% PPI construct collapsed, whereas all PPI-containing constructs retained identifiable three-dimensional shapes. Post-heating lateral deformation decreased with increasing PPI concentration, and the 10% PPI formulation exhibited width and length retention values closest to 100%. In addition, the 7 and 10% PPI formulations showed significantly lower cooking loss than the 3 and 5% formulations. These findings demonstrate that the hydrogel-to-oleogel ratio established the printing characteristics of the bigels, whereas PPI concentration modulated their initial dimensional fidelity and post-heating stability. The developed bigels have potential as 3D-printable structured lipid systems for fat-replacement applications.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2607038"},"PeriodicalIF":3.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal Epithelial-Protective Effects of <i>Leuconostoc mesenteroides</i> H40 in LPS-Stimulated HT-29 Cells.","authors":"Yu-Jin Cha, Hye Ji Jang, Gil-Woong Lee, Na-Kyoung Lee, Hyun-Dong Paik","doi":"10.4014/jmb.2607.07028","DOIUrl":"10.4014/jmb.2607.07028","url":null,"abstract":"<p><p>In this study, the intestinal epithelial-protective effects of <i>Leuconostoc mesenteroides</i> H40 (H40) were investigated using HT-29 intestinal epithelial cells. H40 reduced nitric oxide production in sodium nitroprusside (SNP)-treated cells and suppressed the lipopolysaccharide (LPS)-induced gene expression of inflammatory mediators, including <i>interleukin-1 beta</i> (<i>IL-1β</i>), <i>IL-6</i>, <i>IL-8</i>, <i>TNF-α</i>, and <i>COX-2</i>. In addition, it attenuated the LPS-induced decrease in intestinal barrier-related genes, including <i>ZO-1</i>, <i>claudin-1</i>, <i>occludin</i>, <i>JAM1</i>, and <i>MUC2</i>, which was further supported by preserved or enhanced protein levels of claudin-1, occludin, and MUC2. Furthermore, H40 attenuated the LPS-induced reductions in TPH1 and SERT expression at both the mRNA and protein levels, suggesting a potential influence on serotonin-related molecular pathways in intestinal epithelial cells. Genome annotation revealed that H40 harbors genes associated with exopolysaccharide biosynthesis, cell wall/membrane-associated functions, cell-surface anchoring, antioxidant defense, tryptophan and aromatic amino acid metabolism, and B-vitamin metabolism, indicating that these genomic features may support its intestinal epithelial-protective potential and gut-brain axis-related functionality. Collectively, these findings revealed that H40 exerts anti-inflammatory, intestinal epithelial-protective effects, accompanied by modulation of intestinal barrier- and serotonin-related markers. These results indicate that H40 may represent a promising probiotic candidate for supporting intestinal epithelial health.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2607028"},"PeriodicalIF":3.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Local Suppression of <i>Dgat2</i> Augments Fatty Acid Oxidation in Skeletal Muscle in High-Fat Diet-Fed Mice.","authors":"Yeo Jiyun, Ju Young Park, Min Gyeong Kwon, Eun Seong Kim, Rae Hyeon Oh, Byoung Boo Seo, So Young Bu","doi":"10.4014/jmb.2607.07008","DOIUrl":"10.4014/jmb.2607.07008","url":null,"abstract":"<p><p>Diacylglycerol acyltransferase 2 (DGAT2) codes an enzyme which synthesize triglyceride by esterifying fatty acid to last portion of diacylglycerol backbone, and contributes to intramyocellular lipid metabolism. Small interfering RNA (siRNA)-mediated knockdown of <i>Dgat2</i> was previously shown to reduce AKT phosphorylation and glucose uptake, decrease fatty acid partitioning into triglycerides, and increase free fatty acid release and oxidation in skeletal muscle cells. The current study aimed to determine whether <i>Dgat2</i> knockdown affects lipid and glucose metabolism in glycolytic muscle (GM) and oxidative muscle (OM) under high-fat diet conditions, consistent with our previous <i>in vitro</i> findings. Male C57BL/6J mice were fed high-fat diet, and treated with <i>Dgat2</i> or control-siRNA, and the effects were compared in different muscle types. Muscle <i>Dgat2</i> suppression reduced intramuscular triglyceride content by up to 38.2% whereas increased circulating triglyceride levels. In addition, decreased <i>Gpat3</i> mRNA levels supported a reduction in lipid esterification capacity. <i>Dgat2</i> suppression increased the integration of C14-tagged fatty acids into acid-soluble metabolites, and altered gene expressions related to glucose utilization; GLUT4 protein were decreased and <i>Pdk2</i> mRNA increased in both GM and OM fibers. GM exhibited decreased AKT phosphorylation about 50%, whereas OM showed no change in AKT phosphorylation. Noticeably OM exhibited reduced <i>Hk2</i> and glycogen accumulation. Together, these findings suggest that muscle <i>Dgat2</i> inhibition redirects fatty acid channeling from triglyceride storage toward oxidation <i>in vivo</i>, with accompanying changes in glucose metabolism-related markers. These results extend our previous cell-based findings to a more physiologically relevant setting, while highlighting distinct response patterns in GM and OM.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2607008"},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13563175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148929838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Sustainable Yeast-Based Single-Cell Protein Production from Renewable Substrates Using Engineering Approaches.","authors":"Ely Yosriah, Grace Evelina, Dian Shofinita, Jeong Jae Lee, Soo Rin Kim","doi":"10.4014/jmb.2607.07033","DOIUrl":"10.4014/jmb.2607.07033","url":null,"abstract":"<p><p>Yeast-based single-cell protein (SCP) has emerged as a promising sustainable protein source due to its high protein content, favorable nutritional profile, and ability to utilize renewable feedstocks. The conversion of food-processing wastes, agricultural by-products, and one-carbon substrates into protein-rich biomass supports waste valorization and circular bioeconomy strategies. However, efficient utilization of these diverse feedstocks remains challenging due to variations in substrate composition and microbial metabolic capacity. Recent advances in metabolic engineering, cell wall engineering, adaptive laboratory evolution, random mutagenesis, and fermentation optimization have improved substrate utilization, biomass formation, and protein accumulation in yeasts. These developments have expanded the potential applications of yeast-based SCP in protein supplements, meat products, flavoring agents, and animal feed. This review provides a comprehensive overview of yeast species for SCP production, renewable feedstock utilization, engineering approaches for enhancing biomass and protein accumulation, and the emerging applications of yeast-based SCP in sustainable food and feed systems.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2607033"},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bacillus subtilis</i> DES-59 Modulates Microbial Community Structure and Aroma Formation in Cigar Fermentation to Improve the Quality of Heat-Not-Burn (HnB) Tobacco.","authors":"Lu-Xin Liang, Ming-Jun Zhu, Lu Zhao","doi":"10.4014/jmb.2605.05012","DOIUrl":"10.4014/jmb.2605.05012","url":null,"abstract":"<p><p>Cigar tobacco is characterized by high protein and low sugar contents, which limits the sensory performance of cigar-flavored heat-not-burn (HnB) products. In this study, Yunxue 6 cigar tobacco powder was fermented with <i>Bacillus subtilis</i> DES-59 for 7 days under solid-state culture at 25°C and 65% relative humidity. Compared with the control group, fermented tobacco exhibited a 10.55% reduction in protein, alongside 30.10% and 47.86% increases in total free amino acids and reducing sugars, respectively. Py-GC/MS analysis at 350°C showed increased release of pyrolytic aroma compounds, with the relative abundances of esters, aldehydes / ketones, and phenols increasing by 86.95%, 58.53%, and 35.69%, respectively. Sensory evaluation of HnB cigarettes manufactured from the fermented tobacco powder showed an overall sensory score improvement from 79.00 to 83.00, mainly driven by enhanced aroma and flavor. High-throughput sequencing demonstrated that inoculation reshaped the bacterial community, increasing the relative abundance of <i>Bacillus</i> from 12.20% to 52.40%. PICRUSt functional prediction suggested an increased functional potential for amino acid and carbohydrate metabolism in the treated group. Overall, inoculation with <i>B. subtilis</i> DES-59 was associated with changes in the chemical composition, bacterial community structure, pyrolysis-derived aroma profile, and sensory characteristics of cigar tobacco powder. These findings provide preliminary evidence supporting the potential application of <i>B. subtilis</i> DES-59 in the fermentation of cigar tobacco powder for the development of cigar-flavored raw materials for HnB products.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2605012"},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics in Cardiometabolic Diseases: Current Evidence, Postbiotic Perspectives, and Future Directions.","authors":"Fan Yang, Wanting Qin, Guoxia Zhang, Xinye Li, Xinyu Yang, Li Zhou, Yanwei Xing","doi":"10.4014/jmb.2605.05001","DOIUrl":"10.4014/jmb.2605.05001","url":null,"abstract":"<p><p>The rise in urbanization and sedentary lifestyles, along with the aging of the global population, is contributing to an increasing burden of life-threatening diseases. The cardiometabolic disease (CMD) spectrum, which includes heart failure, hypertension, coronary heart disease, and diabetes, is closely associated with metabolic syndrome components such as obesity and elevated hepatic lipid accumulation. The gut microbiome mediates these disorders through multiple signaling axes. Imbalance of the intestinal microbiota is closely linked to the pathogenesis of atherosclerosis and hypertensive vascular remodeling. Supplementation with probiotics - and, more recently, their derived postbiotic products - can alter the composition and functional metabolic outputs of the gut ecosystem, offering a mechanistically informed approach for CMD management. This review delineates the mechanisms of action of six well-studied probiotics (<i>Akkermansia muciniphila</i>, <i>Bifidobacterium</i>, <i>Lactobacillus</i>, <i>Bacillus</i>, <i>Enterococcus</i>, and <i>Lactococcus lactis</i>), with selected discussion of their postbiotic derivatives where evidence is available. We further examine how antimicrobial and metabolic interventions integrated through the gut-heart-liver axis may improve clinical outcomes and attenuate disease progression. The advancement of precision cardiometabolic medicine through the combined effects of these therapies may facilitate the development of personalized treatment strategies.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2605001"},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal circ-ZNF652 Mediates TGF-β1-Induced Epithelial-Mesenchymal Transition and Glycolytic Reprogramming to Promote Hepatocellular Carcinoma Progression.","authors":"Xianli Niu, Xiaomin Zhang, Guangxia Liang, Nina Wei, Haibo Peng, Xiangyang Li","doi":"10.4014/jmb.2606.06001","DOIUrl":"10.4014/jmb.2606.06001","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC) progression involves epithelial-mesenchymal transition (EMT) and glycolytic reprogramming induced by TGF-β1. The role of exosomal circular RNAs in coordinating these processes remains unclear. This study aims to investigate whether exosomal circ-ZNF652 is associated with TGF-β1-induced mesenchymal marker induction and glycolysis in HCC and elucidate the underlying mechanism. Exosomes were isolated from SMMC-7721 cells and characterized by TEM, NTA, and CD63 western blot. RT-qPCR measured circ-ZNF652 expression. Cell viability, migration, cycle, and apoptosis were assessed by MTT, wound healing, and flow cytometry. EMT and glycolysis proteins were detected by western blot. Glucose uptake, lactate, and HK/LDHA activities were measured. <i>In vivo</i> xenograft and rescue experiments with AKT/HIF-1α inhibitors were performed. TGF-β1 stimulation upregulated circ-ZNF652 in SMMC-7721 cells and their exosomes. Exosomal circ-ZNF652 was internalized by HCC cells and further enhanced TGF-β1-induced upregulation of EMT markers (Twist1, N-cadherin, vimentin, MMP-9). It also promoted cell proliferation, migration, S-phase entry, reduced apoptosis and Caspase-3 activity. Moreover, Exos circ-ZNF652 augmented TGF-β1-induced glycolysis as evidenced by increased glucose uptake, lactate secretion, HK/LDHA activities, and Glut-1/HIF-1α expression. <i>In vivo</i>, circ-ZNF652 overexpression significantly increased tumor volume (1286.5 vs 452.8 mm³) and weight (1.32 vs 0.48 g), along with elevated Ki-67, N-cadherin, HIF-1α, and p-AKT. Rescue experiments showed that AKT or HIF-1α inhibition reversed circ-ZNF652-mediated EMT and glycolysis. All differences were statistically significant (<i>P</i><0.05). To sum up, exosomal circ-ZNF652 is associated with TGF-β1-induced mesenchymal phenotype acquisition and glycolysis via the AKT/HIF-1α pathway, promoting HCC progression. It represents a potential non-invasive biomarker and therapeutic target for HCC.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2606001"},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SCFA Alterations in IBS Subtypes: Systematic Evidence and Future Prospects for Biomarker-Based Diagnosis in India.","authors":"Sanika Limaye, Prajkta Bhide, Anup Kale, Neha Bokey","doi":"10.4014/jmb.2605.05029","DOIUrl":"10.4014/jmb.2605.05029","url":null,"abstract":"<p><p>Irritable bowel syndrome (IBS) is a chronic disorder of gut-brain interaction characterized by abdominal pain and altered bowel habits. Short-chain fatty acids (SCFAs), microbial metabolites of dietary fiber, play key roles in gut physiology and have been implicated in IBS pathogenesis. Propionate, acetate, and butyrate are of particular interest as potential biomarkers for diagnosis and subtype differentiation. A systematic search of PubMed, Web of Science, and Embase identified studies reporting fecal SCFA concentrations in IBS patients versus healthy controls (HCs). Adult cohorts diagnosed by Rome criteria, case-control or randomized designs, and sufficient SCFA data for effect size calculation were included. Data were harmonized to mean ± SD and analyzed; pooled standardized mean differences (SMDs) with 95% confidence intervals (CI) were calculated, with subgroup analyses for IBS-D and IBS-C. Methodological quality was assessed using the Newcastle-Ottawa Scale (NOS). Eleven studies met inclusion criteria. Across all IBS cohorts, propionate was significantly elevated compared to HCs, while acetate and butyrate showed no differences. In IBS-D, propionate was consistently higher. In IBS-C, total SCFAs were significantly reduced, with negligible heterogeneity. Acetate, propionate, and butyrate remained non-significant, with moderate to substantial heterogeneity. NOS assessment indicated that most studies were of good quality, with one rated moderate. This meta-analysis highlights propionate elevation as a biomarker for IBS, particularly IBS-D, and reduced total SCFAs as a distinct metabolic signature in IBS-C. Variability in individual metabolites reflects dietary and methodological influences. The absence of Indian cohorts limits generalizability; future studies integrating SCFA profiling with microbiome analysis in Indian populations are needed to validate biomarker utility and support precision management of IBS.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2605029"},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}