Journal of microbiology and biotechnology最新文献_第3页

Differential Impact of Spike Protein Mutations on SARS-CoV-2 Infectivity and Immune Evasion: Insights from Delta and Kappa Variants.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-12-02 DOI: 10.4014/jmb.2411.11001

Tae-Hun Kim, Sojung Bae, Jinjong Myoung

{"title":"Differential Impact of Spike Protein Mutations on SARS-CoV-2 Infectivity and Immune Evasion: Insights from Delta and Kappa Variants.","authors":"Tae-Hun Kim, Sojung Bae, Jinjong Myoung","doi":"10.4014/jmb.2411.11001","DOIUrl":"10.4014/jmb.2411.11001","url":null,"abstract":"SARS-CoV-2 continues to pose a global health challenge due to its high transmissibility and mutability, with new variants emerging that potentially undermine vaccination and therapeutic efforts. Mutations in the spike protein, particularly in the receptor-binding domain (RBD), significantly influence viral transmissibility and immune escape. However, the complex interplay of these mutations and their combined effects on viral fitness remain to be analyzed. In this study, we investigated the functional impact of key mutations found in the Delta and Kappa variants of SARS-CoV-2. Using pseudovirus assays, we demonstrated that the T478K and L452R mutations characteristic of the Delta variant primarily enhance viral infectivity, with minimal effect on antibody-mediated neutralization. Conversely, the E484Q mutation of the Kappa variant, alone or in combination with L452R, significantly improved evasion of antibody-mediated neutralization but appeared to compromise viral fitness and infectivity. Notably, contrary to previous reports, we found that the P681R mutation contributed neither to increased infectivity nor immune evasion at least in the assay system employed in this study. Our findings suggest that the Delta variant's global dominance over the Kappa variant may be attributed to its superior infectivity and transmissibility rather than enhanced immune evasion capabilities. These results provide valuable insights into the functional consequences of spike protein mutations and may aid in predicting the emergence and spread of future SARS-CoV-2 variants. Such understanding is crucial for enhancing public health preparedness and informing the development of next-generation vaccines and therapeutics.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2506-2515"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Review of Naringenin, a Promising Phytochemical with Therapeutic Potential. 柚皮苷--一种具有治疗潜力的植物化学物质--综述。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-22 DOI: 10.4014/jmb.2410.10006

Jun Hong Shin, Seung Ho Shin

引用次数: 0

Anti-Inflammatory Activity of Biotransformed Platycodon grandiflorum Root Extracts Containing 3-O-β-D-Glucopyranosyl Platycosides in LPS-Stimulated Alveolar Macrophages, NR8383 Cells. 含 3-O-β-D-Glucopyranosyl Platycosides 的生物转化桔梗根提取物在 LPS 刺激的肺泡巨噬细胞 NR8383 细胞中的抗炎活性

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-31 DOI: 10.4014/jmb.2408.08005

Jeong Won Choi, Hyeok Jin Choi, Chae Sun Na, Hwan Lee, Byung Joo Lee, Kyung-Chul Shin, Jin Boo Jeong

{"title":"Anti-Inflammatory Activity of Biotransformed Platycodon grandiflorum Root Extracts Containing 3-O-β-D-Glucopyranosyl Platycosides in LPS-Stimulated Alveolar Macrophages, NR8383 Cells.","authors":"Jeong Won Choi, Hyeok Jin Choi, Chae Sun Na, Hwan Lee, Byung Joo Lee, Kyung-Chul Shin, Jin Boo Jeong","doi":"10.4014/jmb.2408.08005","DOIUrl":"10.4014/jmb.2408.08005","url":null,"abstract":"Acute lung injury (ALI) is a severe inflammatory condition characterized by excessive immune responses and oxidative stress, leading to significant tissue damage. Given the need for novel therapeutic agents, this study aimed to explore the anti-inflammatory activity and mechanisms of biotransformed Platycodon grandiflorum root extracts (BT-PGR), which were enzymatically processed using rapidsase PL Classic from Aspergillus niger. The goal was to assess the potential of BT-PGR as a natural treatment for ALI. BT-PGR effectively inhibited the production of NO, iNOS, IL-1β, IL-6, and TNF-α induced by LPS in NR8383 cells. BT-PGR inhibited the phosphorylation of ERK1/2, p38, JNK and p65 in LPS-stimulated NR8383 cells. In addition, BT-PGR suppressed LPS-mediated activation of NF-κB luciferase activity. BT-PGR increased the levels of HO-1 and the inhibition of HO-1 by ZnPP attenuated BT-PGR-mediated inhibition of NO production. In addition, the inhibition of PI3K by LY294002 blocked the BT-PGR-mediated increase of HO-1 level. BT-PGR increased nuclear Nrf2 level and the knockdown of Nrf2 by siRNA inhibited BT-PGR-mediated increase of HO-1 level. In addition, inhibition of PI3K by LY294002 suppressed the increase of nuclear Nrf2 level. Based on these results, it can be inferred that BT-PGR exhibits anti-inflammatory activity in rat alveolar macrophages, suggesting its potential as a natural candidate for the improvement of ALI.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2609-2617"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ischemic Stroke Induces ROS Accumulation, Maladaptive Mitophagy, and Neuronal Apoptosis in Minipigs.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-14 DOI: 10.4014/jmb.2409.09003

Jie Chen, Yanan Bie, Yajin Guan, Wen Liu, Fei Xu, Tianping Liu, Zilong Meng, Mengqi Gao, Jiawei Liu, Shuilin Xie, Weiwang Gu

{"title":"Ischemic Stroke Induces ROS Accumulation, Maladaptive Mitophagy, and Neuronal Apoptosis in Minipigs.","authors":"Jie Chen, Yanan Bie, Yajin Guan, Wen Liu, Fei Xu, Tianping Liu, Zilong Meng, Mengqi Gao, Jiawei Liu, Shuilin Xie, Weiwang Gu","doi":"10.4014/jmb.2409.09003","DOIUrl":"10.4014/jmb.2409.09003","url":null,"abstract":"Reactive oxygen species (ROS)-induced adaptive/maladaptive mitophagy plays an essential role in the pathophysiology of acute ischemic stroke (AIS). However, most studies have been conducted using rodent models, which limits their clinical application. In this study, we aimed to develop porcine models of permanent stroke and observe the pathophysiological alterations caused by acute ischemic stroke, focusing on ROS-induced mitophagy. Miniature pigs were subjected to lateral frontotemporal electrocoagulation, which resulted in permanent middle cerebral artery occlusion. We investigated global brain damage and mechanisms of adaptive/maladaptive mitophagy caused by ROS and global brain inflammation after AIS. An early neuroinflammatory response was observed in the ipsilateral hemisphere. ROS levels were significantly elevated in the ipsilateral hemisphere and slightly elevated in the contralateral hemisphere. ROS accumulation may be attributed to the increased production and impaired elimination of ROS. In addition, mitophagy and apoptosis were detected in the ischemic core, which may be attributed to ROS accumulation. We propose \"distinct-area targeting\" interventions aimed at maladaptive mitophagy within the ischemic core of the infarct hemisphere, which may provide new therapeutic targets for the treatment of AIS.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2648-2661"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Changes in Microbiome and Metabolome during Sun-Drying of Oysters (Crassostrea gigas), a Traditional Procedure in South China.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-07-17 DOI: 10.4014/jmb.2312.12033

Jiannan Liu, Mingjia Yu, Xiaobo Wang, Ming Qi, Minfu Wu

{"title":"Dynamic Changes in Microbiome and Metabolome during Sun-Drying of Oysters (Crassostrea gigas), a Traditional Procedure in South China.","authors":"Jiannan Liu, Mingjia Yu, Xiaobo Wang, Ming Qi, Minfu Wu","doi":"10.4014/jmb.2312.12033","DOIUrl":"10.4014/jmb.2312.12033","url":null,"abstract":"Sun-drying constitutes a traditional method employed in the preparation of dried oysters within the coastal regions of South China. However, its ramifications on nutritional attributes and the genesis of flavor-contributory compounds in the resultant dried oysters remain significantly unexplored. This research endeavors to scrutinize the repercussions of the production process on the microbiota and metabolites within dried oysters. Utilizing 16s rRNA amplicon sequencing, the identification of 409 operational taxonomic units (OTUs) ensued, wherein Proteobacteria, Bacteroidetes, Firmicutes, Tenericutes, and Actinobacteria surfaced as the primary pathogenic bacteria present in oyster samples. Analysis of the dried oyster sample metabolomes via LC-MS unveiled a discernible augmentation in compounds associated with steroid hormone biosynthesis, arachidonic acid metabolism, biosynthesis of unsaturated fatty acids, and linoleic acid metabolism throughout the entirety of the drying process. Subsequent exploration into the association between metabolites and bacterial communities highlighted the prevailing coexistence of Mycoplasma, Psychrilyobacter, and Vibrio demonstrating negative correlations with a substantial number of metabolites across categories including organic acid and its derivatives, nucleotide and its metabolites, free fatty acids, and amino acids. Conversely, Shewanella and Arcobacter exhibited positive correlations with these metabolite categories. This exhaustive investigation offers invaluable insights into the dynamic alterations within the microbiota and metabolites of dried oysters across diverse drying periods. These findings are anticipated to significantly contribute to the advancement of production techniques and the formulation of enhanced safety measures for the processing of dried oysters.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2555-2564"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dihydromyricetin Alleviates Non-Alcoholic Fatty Liver Disease by Modulating Gut Microbiota and Inflammatory Signaling Pathways.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-20 DOI: 10.4014/jmb.2406.06048

Limin Kang, Xiaolong Ma, Fakun Yu, Lei Xu, Li Lang

{"title":"Dihydromyricetin Alleviates Non-Alcoholic Fatty Liver Disease by Modulating Gut Microbiota and Inflammatory Signaling Pathways.","authors":"Limin Kang, Xiaolong Ma, Fakun Yu, Lei Xu, Li Lang","doi":"10.4014/jmb.2406.06048","DOIUrl":"10.4014/jmb.2406.06048","url":null,"abstract":"Non-alcoholic fatty liver disease (NAFLD) is a prevalent chronic liver condition that is strongly linked to gut microbiota imbalance and chronic inflammation. This study aims to explore the preventive effects of dihydromyricetin (DHM) on NAFLD by modulating the intestinal flora and the TLR4/NF-κB signaling pathway. Fifty male C57BL/6J mice were randomly assigned to five groups: a normal control group, a model group, and three DHM treatment groups receiving low (500 mg/kg), medium (750 mg/kg), and high doses (1,000 mg/kg). NAFLD was induced using a high-fat diet, and DHM was administered for 8 weeks. ELISA measured serum levels of LPS, IL-1β, and TNF-α, while Western Blot assessed liver expression of TLR4 and NF-κB p65. Changes in intestinal flora composition were analyzed using high-throughput 16S rRNA sequencing. The results showed that DHM treatment significantly reduced serum levels of LPS, IL-1β, and TNF-α, decreasing the liver expression of TLR4 and NF-κB p65. Intestinal flora analysis indicated a notable increase in beneficial bacteria, especially in the medium and high-dose groups. DHM treatment also significantly improved liver pathology, reducing fat deposition and inflammatory cell infiltration. In conclusion, DHM effectively prevents the progression of NAFLD by improving gut microbiota balance and suppressing inflammatory signaling pathways.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2637-2647"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Inflammatory Effect of Extract from Fragaria ananassa Duch. Calyx via MAPK and NF-κB Signaling Pathway. 通过 MAPK 和 NF-κB 信号通路从 Fragaria ananassa Duch.花萼提取物通过 MAPK 和 NF-κB 信号通路的抗炎作用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-31 DOI: 10.4014/jmb.2409.09044

Hyo-Min Kim, Dan-Hee Yoo, Jung-Wook Kang, In-Chul Lee, Jong-Sup Bae

{"title":"Anti-Inflammatory Effect of Extract from Fragaria ananassa Duch. Calyx via MAPK and NF-κB Signaling Pathway.","authors":"Hyo-Min Kim, Dan-Hee Yoo, Jung-Wook Kang, In-Chul Lee, Jong-Sup Bae","doi":"10.4014/jmb.2409.09044","DOIUrl":"10.4014/jmb.2409.09044","url":null,"abstract":"Currently, Fragaria ananassa Duch. are discarded as by-products except for the fruit part, so we developed a natural material using the top (= calyx), one of the by-products, and prepared an extract using 70% ethanol to investigate its effects on anti-inflammatory mechanisms. The polyphenol content of 70% ethanol extracts from Fragaria ananassa Duch. calyx was measured to be 265.86 ± 0.85 mg TAE/100 g, respectively. The antioxidant activity was confirmed through the electron donating ability and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging ability measurements. When extracts from Fragaria ananassa Duch. calyx was treated to LPS-induced RAW 264.7 cells, it was confirmed that the production of inflammation-related factors, NO, PGE2, iNOS, COX-2, TNF-a, and IL-6, was inhibited. In addition, it was confirmed that extracts from Fragaria ananassa Duch. calyx affected the MAPK signaling pathway by reducing the protein expression of p-ERK, p-JNK, and p-p38, which are the upper signaling pathways. In addition, it was confirmed to reduce the protein expression of p-p65 and p-IκB, which are NF-κB signaling pathways. Therefore, this study suggests that extracts from Fragaria ananassa Duch. calyx affect the regulation of the production of major inflammation-related factors by inhibiting the MAPK and NF-κB signaling pathway. These results confirmed that extracts from Fragaria ananassa Duch. calyx have the potential to be developed as a new natural material with anti-inflammatory activity.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2662-2674"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Bacterial Therapies and Genetic Engineering Approaches in Colorectal Cancer: A Review of Emerging Strategies and Clinical Implications. 大肠癌的创新细菌疗法和基因工程方法：新策略和临床意义综述》。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-09-25 DOI: 10.4014/jmb.2408.08026

Chunxiao Song, Chunwu Zhao

{"title":"Innovative Bacterial Therapies and Genetic Engineering Approaches in Colorectal Cancer: A Review of Emerging Strategies and Clinical Implications.","authors":"Chunxiao Song, Chunwu Zhao","doi":"10.4014/jmb.2408.08026","DOIUrl":"10.4014/jmb.2408.08026","url":null,"abstract":"Colorectal cancer (CRC) is considered a widespread cancer, ranking second in mortality and incidence among cancer patients worldwide. CRC develops from adenoma to carcinoma through the dynamic interplay of genetic and environmental factors. The conventional modes of treatment, including operation, chemotherapy, and irradiation, are associated with significant challenges, such as drug resistance and toxicity, necessitating the exploration of new treatment modalities. These difficulties reveal the necessity of the emergence of new therapeutic approaches. This review mainly emphasizes the bacterial-based therapies that have recently developed like the engineered bacteriophage therapy and bacterial immunotherapy that pale the existing chemotherapy in terms of toxicity but are effective in killing tumor cells. Also, it also investigates various molecular genetic engineering strategies such as CRISPR-Cas9, CRISPR prime editing and gene silencing to achieve better targeting of CRC. Implementing these new approaches into the forefront of CRC treatment may bring better, more effective therapy with fewer side effects on patients' quality of life.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2397-2412"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ammonia Exposure-Induced Immunological Damage in Chicken Lymphoid Organs via TLR-7/MYD88/NF-κB Signaling Pathway and NLRP3 Inflammasome Activation. 氨暴露通过 TLR-7/MYD88/NF-κB 信号通路和 NLRP3 炎症小体激活诱导鸡淋巴器官的免疫损伤

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-22 DOI: 10.4014/jmb.2407.07025

Mujeeb Ur Rahman, Muhammad Wajid Ullah, Sehrish Manan, Hazrat Bilal, Thamir Alomairy, Mohamed F Awad, Said Nawab, Zahoor, Daochen Zhu

{"title":"Ammonia Exposure-Induced Immunological Damage in Chicken Lymphoid Organs via TLR-7/MYD88/NF-κB Signaling Pathway and NLRP3 Inflammasome Activation.","authors":"Mujeeb Ur Rahman, Muhammad Wajid Ullah, Sehrish Manan, Hazrat Bilal, Thamir Alomairy, Mohamed F Awad, Said Nawab, Zahoor, Daochen Zhu","doi":"10.4014/jmb.2407.07025","DOIUrl":"10.4014/jmb.2407.07025","url":null,"abstract":"Ammonia (NH3) is a hazardous gas that pollutes the environment and causes irritation. Its harmful effects on chickens, including its impact on their immune system, have previously been observed. However, the mechanism by which NH3 exposure causes immune system disorders in chickens remains unclear. The bursa of Fabricius (BF) and thymus are the two main lymphoid organs responsible for the proliferation, differentiation, and selection of B- and T-lymphocytes, both critical for the innate immune response of the host. In this study, we investigated the mechanism of NH3-induced immune dysregulation in broiler chickens. Transmission electron microscopy (TEM) revealed swollen mitochondria and breakage of the large crista lining, membrane deformation, chromatin condensation, increased vacuolation, and blood vessel spasms in the NH3-exposed BF and thymus tissues. Immunofluorescent analysis showed clustering of CD4+ and CD8+ cells, indicating an active immune response to NH3 exposure. Furthermore, NH3 exposure enhanced the mRNA expressions of Toll-like receptor 7 (TLR-7), myeloid differentiation primary response 88 (MYD88), and nuclear factor-kappa B (NF-κB), along with their proteins, and led to activation of the TLR-7/MyD88/NF-κB signaling pathway and NLRP3 inflammasome in chicken thymus tissues. Both mRNA and protein levels of key inflammation-related genes and proteins were upregulated in the NH3-treated group, highlighting a robust inflammatory response due to NH3 exposure. The specific findings of significant structural damage to key lymphoid organs and activation of inflammatory pathways in broiler chickens upon NH3 exposure can provide guidance for future, targeted therapies to improve poultry health.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2527-2538"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Endophytic Streptomyces rhizosphaericola Ahn75 and Its Potential for Biocontrol against Rice Blast. 根瘤链霉菌 Ahn75 的内生特性及其对水稻纹枯病的生物防治潜力。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-10-22 DOI: 10.4014/jmb.2407.07018

Zujiao Fu, Rong Xiao, Zhan Hu, Min Zhang, Shandong Wu, Zhaohui Guo, Rongjun Luo, Shiping Shan, Hua Yang

{"title":"Characterization of Endophytic Streptomyces rhizosphaericola Ahn75 and Its Potential for Biocontrol against Rice Blast.","authors":"Zujiao Fu, Rong Xiao, Zhan Hu, Min Zhang, Shandong Wu, Zhaohui Guo, Rongjun Luo, Shiping Shan, Hua Yang","doi":"10.4014/jmb.2407.07018","DOIUrl":"10.4014/jmb.2407.07018","url":null,"abstract":"Plant endophyte Streptomyces are excellent candidates as biocontrol agents against the rice blast fungus, Magnaporthe oryzae. In this study, a novel strain Ahn75 with antifungal activity was isolated from healthy rice stem and identified as Streptomyces rhizosphaericola by phenotypic characterization and phylogenetic analysis based on 16S rRNA gene, multilocus and genome sequences. Inhibition test using culture filtrate showed that Ahn75 could effectively suppress M. oryzae, with mycelia growth inhibition rate of 80.88% and spore germination inhibition rate of 78.26%. Genome sequence analysis of strain Ahn75 showed 40 gene clusters of secondary metabolites and several genes related to plant growth promotion were predicted in the genome of Ahn75. Several antimicrobial compounds including valinomycin, tetrabutylammonium, and benzalkonium chloride, were also detected in the antifungal fraction from Ahn75 culture filtrate by liquid chromatography and high resolution mass spectrometry. Meanwhile, strain Ahn75 demonstrates UV tolerance under UV irradiation for 60 min, pH tolerance between pH6 and pH9, and a high halotolerance in 7% (w/v) of NaCl. Greenhouse experiments indicated that Ahn75 is able to colonize rice stems, roots, and leaves, which help rice to reduce the rice leaf blast incidence by 59.76%. All these findings suggest that strain Ahn75 could be a potential biocontrol agent for rice blast.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2516-2526"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0