Journal of microbiology and biotechnology最新文献

{"title":"Carotenoid-Producing <i>Qipengyuania algicola</i> sp. nov. and <i>Qipengyuania rhodophyticola</i> sp. nov., Isolated from Marine Algae, and Emended Description of the Genus <i>Qipengyuania</i> Xu <i>et al</i>. 2020.","authors":"Jae Kyeong Lee, Min Woo Lee, Chae Yeong Moon, Jeong Min Kim, Hülya Bayburt, Byeong Jun Choi, Che Ok Jeon","doi":"10.4014/jmb.2507.07023","DOIUrl":"10.4014/jmb.2507.07023","url":null,"abstract":"<p><p>Two Gram-stain-negative, facultatively aerobic, non-motile, catalase- and oxidase-positive, rod-shaped bacteria, designated strains DGS2-2^T (orange-pigmented) and DGS5-3^T (yellow-pigmented), were isolated from marine red algae collected in Korea. Strain DGS2-2^T grew at 20-40°C, pH 6.0-10.0, and in 1.0-6.0% (w/v) NaCl, while DGS5-3^T grew at 15-40°C, pH 6.0-10.0, and in 2.0-6.0% NaCl. Ubiquinone-10 was the sole respiratory quinone. The G+C contents were 62.5% for DGS2-2^T and 57.5% for DGS5-3^T. Both strains contained summed feature 8 (C_18:1<i>ω</i>7<i>c</i> and/or C_18:1<i>ω</i>6<i>c</i>) and C_16:0 as major fatty acids, and phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as major polar lipids. The 16S rRNA gene similarity (97.3%), average nucleotide identity (ANI, 72.0%), and digital DNA-DNA hybridization (dDDH, 18.4%) values between the two strains were below the species delineation thresholds. Phylogenetic and phylogenomic analyses based on 16S rRNA gene and whole-genome sequences placed both strains in distinct lineages within the genus <i>Qipengyuania</i>. ANI and dDDH values between each strain and <i>Qipengyuania</i> type strains were below 74.5% and 19.5%, respectively, supporting their designation as novel species. Genomic analyses identified putative genes associated with potential algal symbiotic traits, including the biosynthesis of vitamins, siderophores, and hormone-like compounds. Carotenoid biosynthetic genes were also identified, and LC/MS confirmed astaxanthin (DGS2-2^T) and nostoxanthin (DGS5-3^T) production. Based on genomic, phylogenetic, phenotypic, and chemotaxonomic evidence, strains DGS2-2^T and DGS5-3^T represent two novel species of <i>Qipengyuania</i>, for which the names <i>Qipengyuania algicola</i> sp. nov. (DGS2-2^T =KACC 23855^T =JCM 37496^T) and <i>Qipengyuania rhodophyticola</i> sp. nov. (DGS5-3^T =KACC 23854^T =JCM 37497^T) are proposed.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507023"},"PeriodicalIF":3.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Antibacterial Effect and Mechanism of a Bacteriocin Produced by <i>Weissella sagaensis</i> ZD-66 against Major Foodborne Pathogens.","authors":"Sijia Sun, Xinling Zhao, Changjiang Wang, Guo Pan, Lirong Yang, Wanli Sha, Wenlong Dong, Baishuang Yin","doi":"10.4014/jmb.2507.07006","DOIUrl":"10.4014/jmb.2507.07006","url":null,"abstract":"<p><p><i>Salmonella</i> Typhimurium is a pathogen bacterium responsible for foodborne diseases. In this study, <i>Weissella sagaensis</i> ZD-66, a bacteriocin-producing strain, was isolated from pickled vegetables. The bacteriocin produced by this strain was characterized, and its antibacterial mechanism was investigated. The genome size of <i>W. sagaensis</i> ZD-66 was 1,862,304 bp, with a GC content of 36.87%. Further, bioinformatic analysis using the BAGEL4 database revealed a class IIa bacteriocin gene cluster, identified as Penocin A. <i>W. sagaensis</i> ZD-66 showed maximum antibacterial activity at 28 h during fermentation. The <i>W. sagaensis</i> ZD-66 was partially purified by using dialysis and Tricine-SDS-PAGE, which revealed a molecular weight of less than 1 kDa. The <i>W. sagaensis</i> ZD-66 showed broad-spectrum inhibition against foodborne pathogens, including <i>S.</i> Typhimurium, <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, <i>Listeria monocytogenes</i>, and <i>Cronobacter sakazakii</i>. The bacteriocin was stable at 121°C for 30 min, pH resistant (pH 3-7), and under UV exposure for up to 2.5 h. However, its antibacterial activity of bacteriocin decreases after treatment with pepsin, trypsin, and pronase E. Furthermore, the treatment with bacteriocin led to the cell membrane being damaged, nucleic acid leakage, and reduced intracellular ATP content. Observation of the ultrastructure of bacteria by Scanning electron microscopy revealed that bacteriocin can inhibit <i>S.</i> Typhimurium by disrupting the cell membrane and pore formation. These results suggest that the bacteriocin produced by <i>W. sagaensis</i> ZD-66 has the potential for a natural food preservative.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507006"},"PeriodicalIF":3.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Domain-Specific Impacts of Spike Protein Mutations on Infectivity and Antibody Escape in SARS-CoV-2 Omicron BA.1.","authors":"Tae-Hun Kim, Sojung Bae, Jinjong Myoung","doi":"10.4014/jmb.2507.07040","DOIUrl":"10.4014/jmb.2507.07040","url":null,"abstract":"<p><p>The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epidemic began in Wuhan, China in late 2019, rapidly spreading worldwide and causing the COVID-19 pandemic. The virus evolved through multiple variants, with Omicron (first detected in late 2021) becoming dominant due to its extensive spike mutations, which enhanced immune evasion despite reduced infectivity compared to earlier strains. Here, we systematically evaluated the functional consequences of these mutations by generating pseudoviruses expressing spike proteins with domain-specific alterations. Mutations in the N-terminal domain (NTD) significantly enhanced pseudoviral infectivity, while receptor-binding domain (RBD) mutations markedly reduced infectivity. Importantly, NTD-mediated enhancement was attenuated when combined with RBD mutations, highlighting a complex interplay between spike regions. Despite lower infectivity compared to Delta, BA.1 pseudoviruses harboring RBD mutations exhibited robust resistance to neutralizing monoclonal antibodies, including casirivimab and imdevimab, with IC₅₀ values exceeding assay limits. These findings indicate that Omicron BA.1's rapid global spread is driven by enhanced immune evasion conferred by RBD mutations, even at the expense of viral entry efficiency. Our domain-specific analysis underscores the critical roles of spike protein mutations in shaping Omicron BA.1's transmissibility and antibody escape, informing strategies for therapeutic and vaccine development.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507040"},"PeriodicalIF":3.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and Genomic Analysis of <i>Leuconostoc citreum</i> DMLC16 Reveals Its Potential as a Probiotic and Antimicrobial Starter.","authors":"Sumin Lee, Minkyeong Kim, Sojeong Heo, Yura Moon, Gawon Lee, Do-Won Jeong","doi":"10.4014/jmb.2507.07004","DOIUrl":"10.4014/jmb.2507.07004","url":null,"abstract":"<p><p>This study aimed to assess the probiotic potential of <i>Leuconostoc citreum</i> DMLC16 previously selected as a fermentation starter candidate based on safety and enzymatic activity evaluations by further examining its health-promoting properties and antimicrobial efficacy. Strain DMLC16 exhibited higher acid tolerance, bile salt resistance, and intestinal adhesion ability than its type strain <i>L. citreum</i> KACC 11860^T. Moreover, it demonstrated antimicrobial activities against a range of foodborne pathogens and spoilage organisms, including <i>Bacillus cereus</i>, <i>Enterococcus faecalis</i>, <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, <i>Alcaligenes xylosoxidans</i>, <i>Flavobacterium</i> sp., and <i>Salmonella enterica</i>. Strain DMLC16 also showed antifungal activities against <i>Clonostachys rosea</i>, <i>Epicoccum nigrum</i>, and <i>Penicillium citrinum</i>. Whole-genome analysis confirmed the safety of DMLC16, revealing the absence of toxin-encoding and plasmids containing acquired antibiotic resistance genes. It also identified genes associated with enzymatic function, salt tolerance, and survival under extreme conditions such as gastric acidity. Although no specific bacteriocin genes were detected, genes implicated in the production of antimicrobial substances were present. The complete genome sequence of DMLC16 provides valuable insight into genetic determinants underlying its probiotic traits and supports its potential application as a functional starter culture or probiotic in the food industry.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507004"},"PeriodicalIF":3.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12438955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin Anti-Aging and Moisturizing Effects of Low-Molecular-Weight Collagen Peptide Supplementation in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial.","authors":"Eunkyoung Lee, Dong Kyu Ahn, Jong Hoon Kim, Sunghee Lee, Han Jo Kim, Hae Kwang Lee, Jin Hee Shin","doi":"10.4014/jmb.2507.07008","DOIUrl":"10.4014/jmb.2507.07008","url":null,"abstract":"<p><p>Skin aging, a multifactorial process influenced by both intrinsic and extrinsic factors, manifests as wrinkles, dryness, reduced elasticity, and altered pore structure. Despite the promising anti-aging effects of low-molecular-weight collagen peptides (LMWCPs), heterogeneity in clinical trial designs poses limitations in fully elucidating their comprehensive efficacy. Therefore, we aimed to evaluate the efficacy and safety of collagen peptide NS (CPNS), a functional food, in improving the biophysical properties related to skin structure and moisture in healthy adults. In this randomized, double-blind, placebo-controlled clinical trial, 70 healthy adults received either test product (1,650 mg/day, including 74.25 mg of the functional peptide, Gly-Pro) or a placebo for 8 weeks, followed by a 2-week washout. Skin parameters were measured using high-precision dermatological devices and expert visual assessments at five time points.The test group showed significant improvements in wrinkle depth, height, and visual severity scores across multiple facial regions. Skin elasticity (R2, R5, and R7), surface and deep skin hydration, as well as dermal density were markedly enhanced. Reductions in the pore number, area, depth, and volume, accompanied by decreased sebum secretion, suggested pore-tightening effects. During the 2-week discontinuation period of test product, significant effects were maintained, and throughout the study duration, no adverse events were reported. Oral administration of test product confers multifaceted benefits to skin health and represents a safe and efficacious functional ingredient to mitigate the visible signs of skin aging.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507008"},"PeriodicalIF":3.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12438954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Mechanistic Study of <i>Lactobacillus helveticus</i> HY7801 and Its Extracellular Vesicles in Premenstrual Syndrome: Role of Gut Microbiota and Hormonal Modulation.","authors":"Hyeonji Kim, Hyeonjun Gwon, Ji-Woong Jeong, Joo-Yun Kim, Jae-Jung Shim, Jae-Hwan Lee","doi":"10.4014/jmb.2507.07014","DOIUrl":"10.4014/jmb.2507.07014","url":null,"abstract":"<p><p>Premenstrual syndrome (PMS) is characterized by periodic physical and psychological symptoms during the menstrual cycle of women of reproductive age. This study aimed to investigate the effects of <i>Lactobacillus helveticus</i> HY7801 (HY7801) on PMS-related hormonal and inflammatory responses, with a specific focus on its influence on the composition of the gut microbiota and the functional role of HY7801-derived extracellular vesicles (EVs). Data from a metoclopramide (MCP)-induced hyperprolactinemia mouse model showed that oral administration of HY7801 reduced serum levels of prolactin and pro-inflammatory cytokines significantly, while restoring hormone imbalances caused by MCP. HY7801 also altered the composition of the gut microbiota by decreasing the relative abundance of <i>Desulfovibrionaceae</i>, <i>Staphylococcaceae</i>, and <i>Bacteroidaceae</i>, which are bacterial families associated with mental health disorders. The abundance of these taxa correlated positively with prolactin and cytokine levels. Furthermore, HY7801-derived EVs were identified as functional bioactive components that exert cytoprotective effects and suppress prolactin secretion significantly in estradiol-stimulated GH3 cells. These findings indicate that HY7801 alleviates PMS symptoms, not only by modulating gut microbiota but also through direct cellular mechanisms mediated by its EVs. Taken together, these dual mechanisms suggest that HY7801 and its secreted components represent a promising approach to management of PMS symptoms.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507014"},"PeriodicalIF":3.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12438957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigation of <i>Bifidobacterium longum</i> Z1 on Methylglyoxal-Induced Glycotoxicity in Neuron-2A Cells by Enhancing Detoxification Pathways.","authors":"Seong-Min Hong, Su-Hyun Kim, Jae-Hyuk Lee, Choong-Hwan Lee, Dong-Hyun Kim, Sun Yeou Kim","doi":"10.4014/jmb.2504.04018","DOIUrl":"10.4014/jmb.2504.04018","url":null,"abstract":"<p><p>Methylglyoxal (MGO), a reactive byproduct of microbial metabolism, contributes to neurodegeneration and may be further exacerbated by gut dysbiosis. Probiotic strategies that restore microbial balance and barrier integrity thus represent a promising therapeutic approach. In this study, we explored the neuroprotective potential of the probiotic strain <i>Bifidobacterium longum</i> Z1 (<i>B. longum</i> Z1) in MGO-challenged mouse neuronal cells (N2a). <i>B. longum</i> Z1 treatment effectively reduced apoptosis and reactive oxygen species (ROS) production, indicating its substantial neuroprotective activity. Mechanistically, <i>B. longum</i> Z1 treatment downregulated apoptotic signaling pathways involving mitogen-activated protein kinases (MAPKs) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Furthermore, <i>B. longum</i> Z1 enhanced cellular detoxification through activation of the glyoxalase system and bolstered antioxidant defenses <i>via</i> induction of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Metabolite analysis identified bioactive compounds within <i>B. longum</i> Z1, notably tryptophan, which exhibited a high affinity for MGO and modulated the expression of apoptosis-related proteins. Our findings indicate that <i>B. longum</i> Z1 and its microbial bioactive metabolites, including tryptophan, may serve as potential nutraceutical candidates for neuroprotection against glycotoxins such as MGO.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504018"},"PeriodicalIF":3.1,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12438956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Safe and Versatile Minicell Platform Derived from <i>Lactiplantibacillus plantarum</i> for Biotechnological Applications.","authors":"Junhyeon Park, Seungjune Chang, Heymin Kang, SangKu Yi, In-Hwan Jang, Kyung-Ah Lee, Donghyun Kim, Juhyun Kim","doi":"10.4014/jmb.2503.07031","DOIUrl":"10.4014/jmb.2503.07031","url":null,"abstract":"<p><p>Bacterial minicells are small and chromosome-free cells that result from aberrant cell division and represent a safe alternative to live microbial applications. However, most research on minicells has focused on <i>Escherichia coli</i>, with few studies exploring their development in non-model, biocompatible hosts. In this study, we engineered a <i>minD</i>-deficient <i>Lactiplantibacillus plantarum</i> (formerly <i>Lactobacillus arabinosus</i> and <i>Lactobacillus plantarum</i>) strain capable of producing minicells and systematically evaluated its potential as a chassis for biotechnological applications. Unlike <i>E. coli</i>-based systems, <i>L. plantarum</i> minicells exhibited stable accumulation of heterologous proteins and efficient surface antigen display without evidence of selective export or stress-induced release of toxic compounds. This behavior enabled uniform protein loading and consistent antigen presentation. Additionally, the minicells retained the immunostimulatory properties of their parent cells, underscoring their potential use as adjuvants <i>per se</i>. To improve production efficiency, we employed a continuous cultivation system with controlled growth conditions, which enabled steady-state operation and significantly enhanced minicell yield at optimal dilution rates. Collectively, these findings establish <i>L. plantarum</i>-derived minicells as a safe, robust, and genetically tunable platform suitable for therapeutic delivery, vaccine development, and immunoengineering.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507031"},"PeriodicalIF":3.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shiga Toxin Type 2 Aggravates G1/S Phase Cell Cycle Arrest, Mediating Caspase-Independent Cell Death under Hyperosmotic Conditions in the Kidney.","authors":"So-Hyeon Park, Kyung-Soo Lee, Jun Young Park, Dae-Soo Kim, Hyun-Soo Cho, Ji Heon Noh, Chang-Ung Kim, Moo-Seung Lee","doi":"10.4014/jmb.2505.08015","DOIUrl":"10.4014/jmb.2505.08015","url":null,"abstract":"<p><p>Shiga toxin (Stx) is a virulence factor produced by <i>Shigella dysenteriae</i> serotype 1 and Stx-producing <i>Escherichia coli</i> (STEC). It causes severe renal damage, leading to hemolytic uremic syndrome (HUS). The main target organ of Stx, the kidney, plays a role in maintaining water homeostasis in the body by increasing an osmotic gradient from the cortex to the medulla. However, the activity of Stxs under kidney hyperosmotic conditions is not well understood. In this study, to investigate the Stx effects under hyperosmotic interstitial fluid, kidney epithelial cells and 3D spheroids were treated with Stx2 and NaCl. Stx2 treatment without NaCl addition increased ER stress, mitochondrial damage, reactive oxygen species, and cleaved caspase 3, 7, while co-treatment with Stx2 and NaCl showed reduced ER-mediated apoptosis. Significantly, NaCl treatment increased HSP70 expression, leading to reduced ER stress by Stx2. Furthermore, cell toxicity by Stx2 and NaCl treatment was increased by the HSP70 inhibitor. Contrariwise, DNA repair gene expression level was decreased, and G1/S phase cell cycle arrest was aggravated after being treated with Stx2 and NaCl than the Stx2 group. Importantly, lactate dehydrogenase and early/late apoptotic cell death were promoted by Stx2 under NaCl treatment. Changes in Stx2 activity at NaCl conditions were confirmed through transcriptome analysis. In conclusion, a hyperosmotic environment changes the cell death mechanism and accelerates the cytotoxicity of Stx, which suggests the importance of studying environmental factors from a pathological perspective and a therapeutic perspective at HUS.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2508015"},"PeriodicalIF":3.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Three-Dimensional Cell Culture Models for Investigating Enterohaemorrhagic <i>Escherichia coli</i> Pathogenesis and Shiga Toxin-Mediated Injury.","authors":"So-Hyeon Park, Mirim Kim, Yu-Jin Jeong, Moo-Seung Lee","doi":"10.4014/jmb.2507.07021","DOIUrl":"10.4014/jmb.2507.07021","url":null,"abstract":"<p><p>Enterohemorrhagic <i>Escherichia coli</i> (EHEC), a pathotype within the Shiga toxin-producing <i>E. coli</i> (STEC) group, is a major etiological agent of severe gastrointestinal illness and life-threatening sequelae, including hemolytic uremic syndrome. Although insights into EHEC pathogenesis have been gained through traditional 2D cell culture systems and animal models, these platforms are limited in their ability to recapitulate human-specific physiological responses and tissue-level interactions. Recent progress in three-dimensional (3D) cell culture systems, such as spheroids, organoids, and organ-on-a-chip (OoC) technologies, has enabled more physiologically relevant <i>in vitro</i> models for investigating host-pathogen dynamics. These advanced platforms offer improved modeling of human tissue architecture, cellular heterogeneity, and microenvironmental complexity, thereby providing novel perspectives on Shiga toxin-mediated damage in intestinal and renal systems. This review synthesizes current applications of 3D culture systems in EHEC research, critically evaluates their advantages and limitations, and outlines future directions for enhancing mechanistic understanding and translational relevance in STEC-associated disease modeling.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507021"},"PeriodicalIF":3.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}