Journal of microbiology and biotechnology最新文献

{"title":"Amelioration of Astrocytic Dysfunction via AQP4/LRP1 Pathway by <i>Zizania latifolia</i> and Tricin in C6 Cells Exposed to Amyloid β and High-Dose Insulin and in Mice Treated with Scopolamine.","authors":"Seun-Ah Yang, Se-Ho Park, Eun-Hye Kim, Won-Bin Bae, Kwang-Hwan Jhee","doi":"10.4014/jmb.2412.12026","DOIUrl":"10.4014/jmb.2412.12026","url":null,"abstract":"<p><p><i>Zizania latifolia</i> and its bioactive compound tricin have been recognized for their anti-inflammatory, anti-allergic, and anti-aging properties. However, the impact of <i>Z. latifolia</i> extract (ZLE) and tricin on astrocyte dysfunction, particularly related to disruptions in the amyloid β (Aβ) clearance pathway, has not been extensively studied. This research aims to explore the regulatory effects of ZLE and tricin on astroglial dysfunction, utilizing astrocytic differentiated C6 cells (passages 75~85) subjected to Aβ and high-dose insulin, as well as scopolamine-induced mice. Results revealed that ZLE (500 μg/ml) and tricin (1 μg/ml) significantly upregulated the expression of astrocyte proteins GFAP and AQP4, brain-derived neurotrophic factor (BDNF), low-density lipoprotein receptor-related protein 1 (LRP1), and matrix metalloproteinases (MMPs) in C6 cells treated with Aβ and high-dose insulin. Furthermore, oral administration of ZLE (100 and 300 mg/kg) and tricin (0.3 mg/kg) in mice led to an increase in acetylcholine (ACh) levels and upregulation of insulin-degrading enzyme (IDE), LRP1, and MMPs, while reducing the levels of acetylcholinesterase (AChE), Aβ and ApoE4. These findings suggest that ZLE and tricin may ameliorate Aβ and high-dose insulin-induced astrocyte dysfunction in C6 cells and scopolamine-treated mice, potentially through the AQP4/LRP1 pathway.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412026"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Metabolomics of <i>Clostridium acetobutylicum</i> ATCC824 and its Engineered Strain, <i>C. acetobutylicum</i> DG1.","authors":"Jae Hyuk Chung, Jieun Lee, Sooah Kim, Kyoung Heon Kim","doi":"10.4014/jmb.2407.07028","DOIUrl":"10.4014/jmb.2407.07028","url":null,"abstract":"<p><p><i>Clostridium acetobutylicum</i>, a strict gram-positive anaerobe, plays a pivotal role in biotechnological applications, particularly in the biosynthesis of 1,3-propanediol, a critical biofuel component and monomer for bioplastic production. This study introduces <i>C. acetobutylicum</i> DG1, a metabolically engineered strain designed to enhance the 1,3-propanediol pathway. Despite its development, comprehensive metabolic comparisons between the parent and modified strains remain unexplored. Our research addresses this gap by employing gas chromatography coupled with time-of-flight mass spectrometry to delineate the global metabolite landscapes of both strains. Through multivariate statistical analysis such as principal component analysis and hierarchical clustering analysis, we discovered pronounced disparities in their metabolite profiles across the acidogenic and solventogenic phases. Detailed metabolomics investigations underscored significant divergences in amino acid metabolism, fatty acid metabolism, and the tricarboxylic acid cycle. These findings shed light on the metabolic alterations induced by genetic engineering in <i>C. acetobutylicum</i>, offering novel insights into microbial metabolism that could guide future biotechnological innovations.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2407028"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of <i>Protosiphon botryoides</i> KNUA219 Isolated from Dokdo Island as a Potential Biofuel Resource.","authors":"Hae-Seo Noh, Jeong-Mi Do, Ho-Seong Suh, Su-Bin Park, Ho-Sung Yoon","doi":"10.4014/jmb.2411.11065","DOIUrl":"10.4014/jmb.2411.11065","url":null,"abstract":"<p><p>The increasing demand for sustainable and eco-friendly energy sources has intensified research into alternative biofuel feedstocks. Microalgae, recognized for their rapid growth and production of high-value products, have emerged as promising candidates for third-generation biofuels. This study evaluates the potential of <i>Protosiphon botryoides</i> KNUA219, a microalga isolated from Dokdo Island, South Korea, as a biodiesel feedstock. Molecular and morphological analyses confirmed its identity, while growth experiments demonstrated its species-specific physiological characteristics, including an optimal pH range of 5-7, limited salinity tolerance, and high biomass productivity. Biochemical analysis revealed significant levels of carbohydrates (30.42 ± 1.65%), proteins (26.18 ± 1.14%), and lipids (14.86 ± 0.33%) in <i>P. botryoides</i> KNUA219, with glucose and galactose as the dominant monosaccharides. Fatty acid methyl ester profiling identified a lipid composition consisting of saturated (20.54%), monounsaturated (19.03%), and polyunsaturated fatty acids (42.65%), with palmitic, oleic, and linolenic acids as key components. Biodiesel quality assessments indicated compliance with critical standards for cetane number and cold filter plugging point, although optimization was required for iodine value and density. Proximate and ultimate analyses revealed favorable energy properties, including a high volatile matter content (88.94 ± 0.33%) and a calorific value of 23.11 ± 0.11 MJ/kg. These findings establish <i>P. botryoides</i> KNUA219 as a promising and sustainable resource for biodiesel production, while highlighting its potential for broader industrial applicability.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411065"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Plant Growth-Promoting (PGP) Native <i>Actinomycetes</i>-Formulated Consortia Mode and Assessed Shelf Life Using Low-Cost Dynamic Media.","authors":"Thirupathi Seenivasa Moorthy, Raju Kannan, Rekha Thiruvengadam, Shine Kadaikunnan, Jamal M Khaled, Baskar Venkidasamy, Muthu Thiruvengadam","doi":"10.4014/jmb.2409.09040","DOIUrl":"10.4014/jmb.2409.09040","url":null,"abstract":"<p><p>Various <i>Actinomycete</i> isolates were collected from the microbial germplasm, including <i>Streptomyces rameus</i> KAC3 (PP550146), <i>Streptomyces bangladeshensis</i> KAC4 (PP177363), <i>Streptomyces mutabilis</i> KAC6 (PP177364), <i>Microbacterium arabinogalactanolyticum</i> KAC8 (PP177365), <i>Streptomyces calvus</i> KAC10 (PP177366), <i>Streptomyces werraensis</i> KAC11(PP177366), and <i>Streptomyces gancidicus</i> KAC12 (PP177389). Three consortia formulations were then selected based on their compatibility with plant growth-promoting rhizobacteria (PGPR) properties under laboratory conditions: C₁ consortium (KAC3, KAC10 and KAC11), C₂ (KAC4, KAC8 and KAC10), and C₃ (KAC11 and KAC12). In this study, we explored the use of locally available, cost-effective carrier materials, such as wood ash (NPK ratio: 1.72:0.86:3.6), charcoal (NPK ratio: 0.93:23.68:0.8), eggshell powder, a surfactant agent (SDS + CTAB), and vermi-compost (NPK ratio: 3.5:0.71:0.6). The CFU counts of <i>Actinomycete</i> isolates in the consortia were calculated over six months at different temperatures (28 and 35 ± 2°C) using these low-cost materials. The C₁ consortium showed the highest stability in maintaining viable populations, whereas C₂ and C₃ showed the best performance in the combined-ingredient carrier material. The efficacy of C₁ was monitored using an IoT device and further validated under different temperature conditions for six months. The data showed that temperature variation affected <i>Actinomycete</i> growth, with an optimal viability at 28 ± 2°C. Moreover, increases in temperature led to higher CO₂ levels and decreased moisture and humidity, yet the <i>Actinomycete</i> population remained stable. This highly efficient C₁ consortium formulation is a promising eco-friendly bioinoculant for sustainable agriculture.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2409040"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Human Cell-Free System for Efficient Protein Production.","authors":"Abbas Mansour, Kalle Kipper, Arto Pulk","doi":"10.4014/jmb.2410.10026","DOIUrl":"10.4014/jmb.2410.10026","url":null,"abstract":"<p><p>We present a highly efficient human HEK293-based cell-free protein synthesis (CFPS) system capable of producing up to 300 μg/ml reporter protein. One of the limitations of the CFPS systems with respect to protein yield has been the decline of the protein-synthesizing activity of the system upon prolonged incubation. Though factors contributing to this decline in activity have been investigated in yeast, little is known about the factors in mammalian systems. We find that a rapid depletion of the components of the energy-regeneration system is a major factor behind the decreasing protein-synthesis activity in the HEK293-derived system. In addition, we demonstrate that a functional CFPS system can be prepared from other mammalian cell lines as evidenced by our use of a human neuroblastoma SH-SY5Y-derived CFPS system. We also find that exogenous creatine kinase (CK) is dispensable for the functionality of the energy-regeneration system in HEK293 due to the presence of a sufficiently high endogenous CK activity in an HEK293 cell-free extract.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2410026"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Miniprotein-Type Inhibitors of Biofilm Formation in <i>Candida albicans</i> and <i>Candida auris</i>.","authors":"Doyeon Kim, Ji-Seok Kim, Xue Bai, Jie Zhang, Minho Park, Ungyu Lee, Jinwook Lee, Yong-Sun Bahn, Yongbin Xu, Nam-Chul Ha","doi":"10.4014/jmb.2411.11076","DOIUrl":"10.4014/jmb.2411.11076","url":null,"abstract":"<p><p><i>Candida auris</i> is a pathogenic fungus associated with high-mortality infections and forms resilient biofilms on various surfaces. In this study, we introduced a novel antifungal strategy against <i>C. auris</i> by integrating an AI-powered protein design tool, ProteinMPNN, with classical molecular dynamics (MD) simulations to design artificial proteins from a miniprotein library. This combined approach accelerated and enhanced the design process, enabling the rapid development of effective miniprotein inhibitors specifically targeting <i>C. auris</i> biofilm formation. The miniproteins developed in this study exhibited potent inhibitory effects on <i>C. auris</i> biofilms, representing a significant advancement in antifungal therapy. Notably, the combined application of these miniproteins enhanced suppression of biofilm formation. These findings highlight not only the strong therapeutic potential of these designed miniproteins but also the power of combining AI-driven protein design with MD simulations to advance biomedical research.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411076"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dsembler - DNA Assembly Designer: A Tool for Facilitating Assembly of Oligomers.","authors":"Aporva Gupta, Gyeongmin Park, So-Yoon Park, Haneul Kim, Eugene Rha, Dae-Hee Lee, Seung-Goo Lee, Haseong Kim","doi":"10.4014/jmb.2412.12046","DOIUrl":"10.4014/jmb.2412.12046","url":null,"abstract":"<p><p>Synthetic biology has garnered significant global interest owing to its diverse applications in bio-based production, biosensing, living therapeutics, and drug delivery. This heightened interest has increased the demand for novel protein synthesis methods and genome-scale assemblies. However, gene assembly from oligomers presents several challenges, including the risk of incorrect assembly between oligomers, self-annealing, and the potential for insertions or deletions resulting from misannealed oligomers. Dsembler (DNA Assembly Designer) is a web-based software application designed to assemble long DNA sequences. It provides a set of oligomers with optimal melting temperatures and GC overlap, which facilitates a commercially available oligomer pool service. By enhancing the accuracy of oligomer design, Dsembler has addressed critical challenges in synthetic biology and supported advancements in genetic engineering and molecular biology.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412046"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Effects of Probiotics and Paraprobiotics Derived from <i>Lactiplantibacillus plantarum</i>, <i>Latilactobacillus sakei</i>, and <i>Limosilactobacillus reuteri</i> in a DSS-Induced Ulcerative Colitis Mouse Model.","authors":"Yun Young Kang, Hyo Jeong Song, So Young Park, Dong Nyoung Oh, Ga Yeong Kim, Na Yeong Been, Da Yeong Kim, Eun Ji Lee, Bo-Hye Nam, Jong-Min Lee","doi":"10.4014/jmb.2411.11045","DOIUrl":"10.4014/jmb.2411.11045","url":null,"abstract":"<p><p>Live biotherapeutic products, represented by probiotics with disease-mitigating or therapeutic effects, face significant limitations in achieving stable colonization in the gut through oral administration. However, paraprobiotics, which consist of dead or inactivated microbial cells derived from probiotics, can provide comparable health benefits while overcoming the limitations associated with live biotherapeutic products. Therefore, the purpose of this study was to quantitatively compare and analyze the effects of probiotics, which are gaining attention as treatments for inflammatory bowel diseases, and their paraprobiotic counterparts on the alleviation of ulcerative colitis. In <i>in vitro</i> evaluations revealed that the paraprobiotics derived from <i>Lactiplantibacillus plantarum</i> MGEL20154, <i>Latilactobacillus sakei</i> MGEL23040, and <i>Limosilactobacillus reuteri</i> MGEL21001 exhibited equal or significantly enhanced activities in terms of antioxidant properties, anti-inflammatory effects, and barrier integrity enhancement compared to their probiotic counterparts. Furthermore, consistent with <i>in vitro</i> findings, both probiotics and paraprobiotics effectively improved histological scores and reduced myeloperoxidase levels in a DSS-induced ulcerative colitis mouse model. Notably, paraprobiotics derived from <i>L. plantarum</i> MGEL20154 and <i>L. reuteri</i> MGEL21001 demonstrated significantly enhanced efficacy in restoring tight junctions, promoting mucin secretion, and reducing inflammation in colonic lesion tissues compared to their probiotic forms. Our results suggest that these paraprobiotics may serve as more suitable agents for alleviating and treating ulcerative colitis, addressing limitations associated with probiotics, such as low survival rates, instability, antibiotic susceptibility, and the potential induction of excessive inflammatory responses.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411045"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Multifaceted Genus <i>Acinetobacter</i>: the Facts, the Concerns and the Oppoptunities the Dualistic Geuns <i>Acinetobacter</i>.","authors":"Tsvetana Muleshkova, Inga Bazukyan, Konstantinos Papadimitriou, Velitchka Gotcheva, Angel Angelov, Svetoslav G Dimov","doi":"10.4014/jmb.2411.11043","DOIUrl":"10.4014/jmb.2411.11043","url":null,"abstract":"<p><p>In recent years, the research community has been interested in members of the <i>Acinetobacter</i> genus mainly because of their role as causative agents of nosocomial infections. However, this rich-in-species genus has been proven to play a significant role in several biotechnological processes, such as bioremediation and fermented foods production. To partially fill the lack of information on <i>Acinetobacter</i>'s dualistic nature, in this review, based on literature data, we attempt to summarize the available information on the different roles the members of the genus play by considering their genetic constitution and metabolic properties. We analyzed reports of genetic divergence between the pathogenic and non-pathogenic species and isolates, which can be explained by their high adaptability to the different ecological niches. In turn, this adaptability could result from intrinsic genetic variability due to mechanisms of horizontal genetic transfer, as well as high mutability determined by the expression of error-prone DNA polymerases. Yet, we concluded that further studies are needed, especially whole-genome sequencing of non-pathogenic isolates, which for the moment are relatively scarce.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411043"},"PeriodicalIF":2.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BlingLife^®-Marigold Extract Alleviates Blue Light-Induced Retinal Mitochondria Oxidative Stress and Senescence by Activating NRF2/HO-1 Signaling.","authors":"Zixiu Zhou, Sizhen Li, Qingsong Yang, Pengjie Zheng, Kexin Xie","doi":"10.4014/jmb.2411.11037","DOIUrl":"10.4014/jmb.2411.11037","url":null,"abstract":"<p><p>Blue light poses a risk of retinal damage with excessive exposure. BlingLife^®-marigold extract (BLM) is an alcohol extract of magrigold, which contains abundant lutein, zeaxanthin and meso-zeaxanthin. This study aimed to explore the role and potential mechanisms of BLM in blue light-induced retinal damage both in vivo and in vitro. Rats or human retinal pigment epithelial cell line (ARPE-19) were exposed to blue LED light with or without BLM treatment. The retinal morphology changes of rat were evaluated by H&E staining. Mitochondrial morphology was examined by using a transmission electron microscope. Besides, mitochondria oxidative stress was evaluated by detecting mitochondrial membrane potential, ROS, MDA and SOD levels. By measuring γH2AX expression and performing SA-β-galactosidase (gal) staining, cell senescence was assessed. Additionally, cell cycle was detected using flow cytometry. Western blot was employed to examine the expression of NRF2 and HO-1. Results indicated that BLM could protect against blue light-induced damage of rat retinal tissues and ARPE-19 cells, as evidenced by the improved histopathological changes, alleviated mitochondria oxidative stress and attenuated senescence of tissues and cells. More importantly, BLM activated NRF2/HO-1 signaling, and addition of NRF2 inhibitor ML385 significantly blocked the protective effects of BLM on ARPE-19 cells exposed to blue light. In conclusion, BLM can provide an effective protection against blue light-induced retinal damage at least partly by activating NRF2/HO-1 signaling, suggesting that BLM may be useful for the prevention of blue light-induced retinal injury.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411037"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}