Journal of microbiology and biotechnology最新文献

{"title":"Hepatoprotective Effect of Meroterpenoid-Rich Fraction from the Ethanolic Extract of <i>Sargassum sarratifolium</i> on <i>tert</i>-Butyl Hydroperoxide-Induced Mice.","authors":"Sujin Lim, Ji Young Hwang, Kyoung Mi Moon, Hyeon Hak Jeong, Jaeseong Seo, Hyeung-Rak Kim, Bonggi Lee","doi":"10.4014/jmb.2501.01014","DOIUrl":"10.4014/jmb.2501.01014","url":null,"abstract":"<p><p>The liver, critical for detoxification and metabolic homeostasis, is highly susceptible to oxidative stress caused by reactive oxygen species (ROS). This study evaluated the hepatoprotective effects of a meroterpenoid-rich fraction (MES) from Sargassum serratifolium in a tert-butyl hydroperoxide (t-BHP)-induced oxidative liver injury model in mice. MES treatment ameliorated t-BHP-induced histopathological changes, including hepatocyte swelling, cytoplasmic vacuolation, and necrosis. Biochemical analyses showed that MES significantly decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities to 70% and 50%, respectively, of the levels observed in the t-BHP-treated group. It also reduced Lactate Dehydrogenase (LDH) activity, which was increased fivefold by t-BHP, by up to 70%, and significantly reduced hepatic Malondialdehyde (MDA) levels. MES restored the activities of antioxidant enzymes including Superoxide Dismutase (SOD), catalase, Glutathione Peroxidase (GPx), and Glutathione Reductase (GR), and activated the Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway, leading to increased glutathione (GSH) levels and enhancing the expression of detoxifying enzymes such as heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1). These findings demonstrate that MES provides effective protection against oxidative liver damage by mitigating oxidative stress, enhancing antioxidant defenses, and activating the Nrf2 signaling pathway. This highlights the potential of marine-derived natural products as hepatoprotective agents.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501014"},"PeriodicalIF":2.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root Rot Disease Biocontrol and Microbiome Community Modulation by <i>Streptomyces</i> Strains in Soybean.","authors":"Da-Ran Kim, Youn Min Ko, Donggyu Lee, Youn-Sig Kwak","doi":"10.4014/jmb.2502.02010","DOIUrl":"10.4014/jmb.2502.02010","url":null,"abstract":"<p><p>Traditionally, phytopathogenic fungi control strategies rely primarily upon chemical fungicides, but fungicide resistance pathogen strains have appeared in the fields. Therefore, biocontrol approaches highlighted with sustainable agriculture aspects, especially the genus <i>Streptomyces</i>, are known to suppress numerous plant diseases. <i>Streptomyces bacillaris</i> S8 was isolated from turfgrass rhizosphere, and <i>Streptomyces globisporus</i> SP6C4 was obtained from strawberry pollen. Both strains showed excellent antifungal and antibacterial activities and suppressed various plant diseases <i>in vitro</i>. However, beneficial microorganisms are rarely studied and introduced to another effect on microbial communities when incompatible with the host. The present study aims to assess the potential of effective control of plant diseases by both strains in new crops and to assess the impact of endogenous microbiota. Various diseases pose significant concerns in soybean production, leading to substantial grain yield and quality losses. Root rot caused by <i>Fusarium</i> spp. is known to be the most problematic disease in the soybean cropping system. In the results, <i>S. globisporus</i> SP6C4 and <i>S. bacillaris</i> S8 showed antifungal activity against soybean root rot pathogen, but strain S8 had less than SP6C4. The strain SP6C4 played a role as hub-taxa in the early stage, and the strain S8 was a modulator in microbial communities. Our results demonstrate the antifungal activity of <i>S. globisporus</i> SP6C4 and <i>S. bacillaris</i> S8, which can be expected to grow and reduce the disease of soybeans. The S8 and SP6C4 can also modify the plant microbiota which may open a new dimension of crop microbiome research.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502010"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Profiling of Tomato Root Exudates Induced by <i>Ralstonia solanacearum</i> Strains of Different Pathogenicity: Screening for Metabolites Conferring Bacterial Wilt Resistance.","authors":"Zheng Chen, Enquan Lin, Xia Lin, Lianlian Liu, Wangyu Li, Junjie Feng, Bo Liu, Xuefang Zheng, Meichun Chen","doi":"10.4014/jmb.2501.01033","DOIUrl":"10.4014/jmb.2501.01033","url":null,"abstract":"<p><p>Tomato is one of the most widely cultivated and consumed vegetables in the world, and its production is severely threatened by bacterial wilt. Bacterial wilt caused by <i>Ralstonia solanacearum</i>, is one of the most devastating plant diseases. <i>R. solanacearum</i> is a complex species with both virulent and avirulent strains. The avirulent strains show high biocontrol activity against bacterial wilt. A metabolomics study was conducted on tomato root exudates induced by <i>R. solanacearum</i> of different pathogenicity, and the potential bacterial wilt resistance metabolites were screened. Principal component analysis revealed a clear separation of the metabolic profile between the virulent strain-induced group, the avirulent strain-induced group, and the CK group. Based on the altered abundance in root exudates after <i>R. solanacearum</i> induction, the most differential metabolites were selected for further investigation, including citramalic acid, glucuronic acid, alpha-ketoglutaric acid, and malic acid, etc. The plate inhibition assay showed that alpha-ketoglutaric acid (AKG) and malic acid (MA) had a dose-dependent inhibitory effect on <i>R. solanacearum</i> (5-20 mg/ml, inhibition circle diameter 14.69-25.08 mm). Crystal violet staining showed that 1-2.5 mg/ml of MA and AKG could significantly inhibit <i>R. solanacearum</i> biofilm formation at 24 h (<i>P</i> < 0.0001, inhibition rate 66.93-70.43%). In tomato pot experiments against bacterial wilt, the AKG group had 75.36% biocontrol efficacy, and the MA group had 57.97% efficacy 25 days after inoculation. We conclude that AKG and MA play an important role in resistance to bacterial wilt in tomato.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501033"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Nasal Vaccine Displaying Anthrax Antigen on the Surface of <i>Lactiplantibacillus plantarum</i> Induces Protective Mucosal Immunity against Anthrax Toxin.","authors":"In-Hwan Jang, In Ryeong Jung, Hyojung Suh, Hyo-Joo Ahn, Donghyun Kim, Youn Soo Choi, June-Yong Lee, Kyung-Ah Lee","doi":"10.4014/jmb.2503.03036","DOIUrl":"10.4014/jmb.2503.03036","url":null,"abstract":"<p><p>Anthrax, particularly inhalational anthrax, poses a severe threat due to its high fatality rate and potential misuse as a biological weapon. Current anthrax vaccines require multiple injections, which limits their practicality and underscores the need for improved, user-friendly vaccination strategies. In this study, we developed a novel nasal booster vaccine platform aimed at preventing inhalational anthrax by inducing mucosal immunity at the pathogen's entry site, the respiratory tract. We successfully engineered a biologically safe <i>Lactiplantibacillus plantarum</i> (<i>L. plantarum</i>) strain to display the anthrax protective antigen (PA) on its surface. Following systemic priming by intramuscular immunization, nasal boosting with this recombinant <i>L. plantarum</i> strain significantly enhanced protective antigen (PA)-specific mucosal IgA production in both nasal and bronchoalveolar lavage fluids. Importantly, mucosal IgA antibodies elicited through this nasal boosting strategy effectively neutralized anthrax lethal toxin. Our findings indicate that recombinant <i>L. plantarum</i> expressing PA represents a promising nasal vaccine booster platform, potentially replacing traditional intramuscular boosters, and paving the way for the development of effective and easily administered mucosal vaccines against anthrax and other respiratory pathogens.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503036"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Ginger on Gut Microbiota Composition and Function in a <i>Bacteroides</i>-Dominant Enterotype.","authors":"Jinwoo Kim, Jina Ha, Seongok Kim, Gyungcheon Kim, Hakdong Shin","doi":"10.4014/jmb.2503.03032","DOIUrl":"10.4014/jmb.2503.03032","url":null,"abstract":"<p><p>Ginger (<i>Zingiber officinale</i>) has been used worldwide for centuries, valued for both its culinary applications and potential therapeutic properties. Its bioactive compounds exhibit antioxidant, anti-inflammatory, and metabolic regulatory effects, providing physiological benefits to the human body. However, its influence on the gut microbiota remains poorly understood. In this study, we investigated the impact of ginger on gut microbiota using an <i>in vitro</i> fecal incubation model. To minimize interindividual variability, we classified participants into enterotypes based on gut microbial composition, focusing on the <i>Bacteroides</i>-dominant enterotype. While ginger treatment did not significantly affect microbial alpha diversity, it induced distinct shifts in bacterial structure, suggesting compositional changes in the microbiota. At the phylum level, taxonomic analysis revealed a lower relative abundance of Bacteroidota and a higher relative abundance of Proteobacteria in the ginger-treated group compared to the control. Consistently, genus-level analysis showed an increased relative abundance of <i>Acinetobacter</i> and <i>Enterobacteriaceae</i>, both belonging to Proteobacteria, in the ginger-treated group. Predicted functional pathway analysis further revealed that ginger treatment enriched pathways related to linoleic acid metabolism, beta-alanine metabolism, geraniol degradation, and tetracycline biosynthesis. These findings suggest that ginger modulates gut microbiota composition, particularly by increasing the abundance of Proteobacteria-associated genera. This enterotype-based study provides a structured framework for evaluating dietary effects and may support the development of personalized dietary strategies targeting gut microbiome modulation.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503032"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesis of Vanadium Dioxide Nanoparticles by <i>Shewanella</i> sp. Strain HN-41.","authors":"Yongseok Ko, Saehyun Kang, Youri Yang, Jisu Lee, Hor-Gil Hur","doi":"10.4014/jmb.2502.02051","DOIUrl":"10.4014/jmb.2502.02051","url":null,"abstract":"<p><p>Vanadium dioxide (VO₂) nanoparticles have various application potentials such as smart windows and electronic devices due to their unique phase transition properties. However, conventional VO₂ synthesis methods require harsh conditions and toxic reducing agents, leading to environmental problems. In this study, we developed an eco-friendly method to biosynthesize VO₂ nanoparticles using <i>Shewanella</i> sp. strain HN-41 under anaerobic conditions at 30°C and neutral pH. Morphological observations revealed that biogenic VO₂ nanoparticles with an average size of 4.3 nm were in the form of granules presented inside and outside the cells. These nanoparticles were identified as VO₂ by differential scanning calorimetry (DSC) analysis, which showed a phase transition temperature of 61.9°C, consistent with that of VO₂. Furthermore, we observed an active formation of vesicles containing VO₂ nanoparticles by the cross-sectioned transmission electron microscopy (TEM) analysis. Thus, in addition to the direct extracellular formation of VO₂ nanoparticles through anaerobic respiration, bacterial membrane vesicles likely play a role in expelling nanoparticles from the cell, potentially mitigating their toxicity. These findings highlight metal reducing bacteria could be a biological green agent for the production of valuable VO₂ nanoparticles under anaerobic environmental conditions.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502051"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the Improvement Effects and Mechanisms of <i>Magnolia sieboldii</i> Essential Oils on Insomnia in Mice.","authors":"Guofeng Shi, Shuanghe Wang, Shanshan Luo, Jiajing Ding, Zixuan Liang, Wenyu Cao, Xiaoyan Li, Yixi Zeng, Yanqing Ma, Lanyue Zhang, Hui Li","doi":"10.4014/jmb.2502.02050","DOIUrl":"10.4014/jmb.2502.02050","url":null,"abstract":"<p><p>Insomnia is a common sleep disorder that is difficult to cure. <i>Magnolia sieboldii</i> essential oils (MSEOs) have been shown to have antidepressant effects, but there are few studies on treating insomnia. Therefore, this study aimed to investigate the therapeutic effects of MSEOs and to elucidate the molecular and neurophysiological mechanisms by which they alleviate insomnia. The main components of MSEOs extracted by steam distillation were analyzed by gas chromatography-mass spectrometry (GC-MS). To establish a p-chlorophenylalanine (PCPA)-induced insomnia model in mice, the levels of GAD65, GABAARα1, 5HT-2A, and 5HT-1A were detected by immunohistochemistry. The normal neurons in the mouse brain were counted by Nissl staining. RT-qPCR detected the relative mRNA expression levels of related genes in mice. A total of 69 components were identified by MSEOs, and the main components were β-elemene (19.94%), (Z)-β-ocimene (14.87%), and Germacrene D (7.05%). Different concentrations of MSEOs can successfully prolong the total sleep time and shorten the sleep latency of mice. GAD65, GABAARα1, 5HT-2A, and 5HT-1A levels still increased to varying degrees after treatment with different concentrations of MSEOs. Moreover, MSEOs could attenuate PCPA-induced neuronal death. At the same time, MSEOs enhanced the mRNA expression of 5HT-2A, GABAARα1, and GABAARγ2. MSEOs can extend sleep duration and reduce sleep latency in mice. MSEOs may demonstrate potential for treating insomnia by promoting neuronal proliferation in the brains of insomniac mice and upregulating the expression of GAD65, GABAARα1, 5HT-1A, and 5HT-2A proteins in various brain regions, potentially becoming an effective candidate for insomnia treatment.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502050"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Catalytic Domains of Glycoside Hydrolase Family 5 from <i>Paenibacillus curdlanolyticus</i> are Important for Promoting Multifunctional Enzyme Activities and Degradation of Agricultural Residues.","authors":"Niendy Virnanda Fatmawati, Apinya Singkhala, Prattana Ketbot, Sirilak Baramee, Rattiya Waeonukul, Chakrit Tachaapaikoon, Ayaka Uke, Akihiko Kosugi, Khanok Ratanakhanokchai, Patthra Pason","doi":"10.4014/jmb.2501.01046","DOIUrl":"10.4014/jmb.2501.01046","url":null,"abstract":"<p><p><i>Pc</i>GH5 from <i>Paenibacillus curdlanolyticus</i> strain B-6 is a modular protein consisting of a catalytic domain of glycoside hydrolase family 5 (GH5), and three non-catalytic domains (a family 11 carbohydrate-binding module (CBM11), a fibronectin type 3 (Fn3), and a family 3 carbohydrate-binding module (CBM3). In this study, the recombinants full-length <i>Pc</i>GH5 and the catalytic domain (<i>Pc</i>GH5_CD) were expressed in <i>Escherichia coli</i> and purified. Most GH5 members exhibit endo-cellulase activity. However, the catalytic domain enzyme of strain B-6 exhibited unique properties, showing multifunctional enzyme activities of endo-cellulase, endo-xylanase, endo-mannanase, and endo-1,3-1,4-β-glucanase. The sequence alignment of <i>Pc</i>GH5_CD compared to other characterized GH5 enzymes suggests that the two catalytic residues and the six substrate-binding subsites of endo-cellulases were conserved with other different GH5 enzyme properties. Whereas a few conserved amino acid residues and/or short peptides located outside the active site of the GH5 endo-cellulases may be involved in broad substrate specificity of <i>Pc</i>GH5_CD enzyme on xylan, mannan and 1,3-1,4-β-glucan. Moreover, the non-catalytic domains (CBM11-Fn3-CBM3) linked to the GH5 catalytic domain are important for promoting the multifunctional enzyme activities of <i>Pc</i>GH5 on the β-1,4 glycosidic linkages of crystalline cellulose, highly branched polysaccharides, and β-1,4-1,6 and β-1,3-1,4 glycosidic linkages of polysaccharides, especially for the polysaccharides complex contained in agricultural residues. The full-length <i>Pc</i>GH5 is effective in producing oligosaccharides from agricultural residues without pretreatment. Therefore, it is interesting to use it as a source of prebiotics producer for use in various food products.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501046"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Mechanisms of <i>Pleurotus citrinopileatus</i>: Inhibition of MAPK and NF-κB Signaling Pathways, and Activation of ROS/PI3K/Nrf2/HO-1 Signaling Pathway in LPS-Stimulated RAW264.7 Cells.","authors":"Hyeok Jin Choi, Jeong Won Choi, So Jung Park, Sang Hun Lee, Jin Hyuk Hwang, Youngki Park, Kyoung Tae Lee, Jin Boo Jeong","doi":"10.4014/jmb.2501.01012","DOIUrl":"10.4014/jmb.2501.01012","url":null,"abstract":"<p><p>This study explores the anti-inflammatory potential of <i>Pleurotus citrinopileatus</i> by examining its impact on inflammation-related signaling pathways in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Fractions obtained using hexane (HE), dichloromethane (DCM), and ethyl acetate (EA) were found to suppress LPS-induced nitric oxide (NO) production and the expression of inducible nitric oxide synthase (iNOS). Additionally, these fractions inhibited the phosphorylation of key mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated kinases 1/2 (ERK1/2), p38, and c-Jun N-terminal kinase (JNK), as well as the nuclear factor kappa B (NF-κB) subunit p65. The HE, DCM, and EA fractions also promoted the nuclear accumulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increased the expression of heme oxygenase-1 (HO-1). Notably, the suppression of HO-1 activity using zinc (II) protoporphyrin IX (ZnPP) reversed the NO-inhibitory effects of these fractions. Furthermore, treatment with the HE, DCM, and EA fractions enhanced phosphoinositide 3-kinase (PI3K) activation, whereas PI3K inhibition by LY294002 attenuated HO-1 expression and nuclear Nrf2 translocation. Reactive oxygen species (ROS) scavenging by N-acetyl-L-cysteine (NAC) similarly reduced PI3K activation and the upregulation of HO-1 and nuclear Nrf2. Collectively, these findings indicate that the HE, DCM, and EA fractions mitigate NO production by downregulating iNOS expression through the suppression of MAPK and NF-κB signaling, while also engaging the ROS/PI3K/Nrf2/HO-1 pathway to exert anti-inflammatory effects.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501012"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling of Metabolite Changes in Lettuce Leaves during Fermentation by <i>Bacillus subtilis</i>.","authors":"Dong Young Lee, Yu Rim Yun, Byeong-Jun Ji, Sewol Park, Subin Kim, Sun-Hee Lee, Hyun-Soo Chun, Eun Ju Yun","doi":"10.4014/jmb.2501.01026","DOIUrl":"10.4014/jmb.2501.01026","url":null,"abstract":"<p><p>Metabolic profiling is a valuable tool for elucidating the biochemical pathways and key metabolites involved in the health benefits associated with microbial fermentation. In this study, we investigated the metabolic changes occurring during the fermentation of lettuce leaves by <i>Bacillus subtilis</i>, a widely studied bacterium known for its diverse metabolic capabilities. Through non-targeted metabolic profiling, we identified and characterized metabolites that may contribute to the beneficial effects of fermented lettuce. Using gas chromatography-mass spectrometry (GC/MS), we identified 54 metabolites in the fermented lettuce samples. Additionally, we elucidated the alterations in metabolite profiles during the bioconversion of lettuce using <i>B. subtilis</i>. Notably, 11,14-eicosadienoic acid, 13-docosenoic acid, and oleic acid were either produced or enriched during bioconversion, were identified as potential contributors to the enhanced nutritional and bioactive properties of fermented lettuce. This study underscores the potential of metabolic profiling to uncover the metabolic pathways and specific metabolites associated with health benefits in fermented foods. These findings pave the way for developing functional foods with improved nutritional value and bioactivity.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501026"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}