Journal of microbiology and biotechnology最新文献_第4页

Using Synbiotics as a Therapy to Protect Mental Health in Alzheimer's Disease. 利用合成益生菌保护阿尔茨海默氏症患者的心理健康。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-06-21 DOI: 10.4014/jmb.2403.03021

Anh Pham Thi Ngoc, Adil Zahoor, Dong Gyun Kim, Seung Hwan Yang

{"title":"Using Synbiotics as a Therapy to Protect Mental Health in Alzheimer's Disease.","authors":"Anh Pham Thi Ngoc, Adil Zahoor, Dong Gyun Kim, Seung Hwan Yang","doi":"10.4014/jmb.2403.03021","DOIUrl":"10.4014/jmb.2403.03021","url":null,"abstract":"Alzheimer's disease (AD) is a progressive neurological disorder that represents a major cause of dementia worldwide. Its pathogenesis involves multiple pathways, including the amyloid cascade, tau protein, oxidative stress, and metal ion dysregulation. Recent studies have suggested a critical link between changes in gut microbial diversity and the disruption of the gut-brain axis in AD. Previous studies primarily explored the potential benefits of probiotics and prebiotics in managing AD. However, studies have yet to fully describe a novel promising approach involving the use of synbiotics, which include a combination of active probiotics and new-generation prebiotics. Synbiotics show potential for mitigating the onset and progression of AD, thereby offering a holistic approach to address the multifaceted nature of AD. This review article primarily aims to gain further insights into the mechanisms of AD, specifically the intricate interaction between gut bacteria and the brain via the gut-brain axis. By understanding this relationship, we can identify potential targets for intervention and therapeutic strategies to combat AD effectively. This review also discusses substantial evidence supporting the role of synbiotics as a promising AD treatment that surpasses traditional probiotic or prebiotic interventions. We find that synbiotics may be used not only to address cognitive decline but also to reduce AD-related psychological burden, thus enhancing the overall quality of life of patients with AD.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of High-Temperature Feeds on Gut Microbiota and MAFLD. 高温饲料对肠道微生物群和 MAFLD 的影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-31 DOI: 10.4014/jmb.2405.05023

Lijun Xue, Kaimin Li, Yanfei Jia, Dongxue Yao, Xuexing Guo, Shuhong Zhang

引用次数: 0

Enhancing Protein Content in Wild-Type Saccharomyces cerevisiae via Random Mutagenesis and Optimized Fermentation Conditions. 通过随机突变和优化发酵条件提高野生型酿酒酵母的蛋白质含量

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-31 DOI: 10.4014/jmb.2405.05027

Sang-Hun Do, Tae-Gi Lee, Sun-Ki Kim

{"title":"Enhancing Protein Content in Wild-Type Saccharomyces cerevisiae via Random Mutagenesis and Optimized Fermentation Conditions.","authors":"Sang-Hun Do, Tae-Gi Lee, Sun-Ki Kim","doi":"10.4014/jmb.2405.05027","DOIUrl":"10.4014/jmb.2405.05027","url":null,"abstract":"Single-cell protein (SCP) derived from microorganisms is widely recognized as a viable alternative protein source for the future. Nevertheless, the commercialization of yeast-based SCP is hampered by its relatively low protein content. Therefore, this study aimed to enhance the protein content of Saccharomyces cerevisiae via random mutagenesis. To achieve this, S. cerevisiae KCCM 51811, which exhibited the highest protein concentration among 20 edible S. cerevisiae strains, was selected as a chassis strain. Subsequently, a KCCM 51811 mutant library was constructed (through UV irradiation) and screened to isolate mutants exhibiting high protein content and/or concentration. Among the 174 mutant strains studied, the #126 mutant exhibited a remarkable 43% and 36% higher protein content and concentration, respectively, compared to the parental strain. Finally, the #126 mutant was cultured in a fed-batch system using molasses and corn-steep liquor, resulting in a protein concentration of 21.6 g/l in 100 h, which was 18% higher than that produced by the parental strain. These findings underscore the potential of our approach for the cost-effective production of food-grade SCP.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antidepressant Effect and Mechanism of Picea mariana Essential Oil on Reserpine-Induced Depression Model Mice. 红豆杉精油对瑞舍平诱导的抑郁模型小鼠的抗抑郁作用及其机制

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-18 DOI: 10.4014/jmb.2405.05013

Ying Wang, Guofeng Shi, Yixi Zeng, Juting Li, Yongyu Wu, Jiahui Zheng, Anjing Xu, Yanqing Ma, Lanyue Zhang, Hui Li

{"title":"Antidepressant Effect and Mechanism of Picea mariana Essential Oil on Reserpine-Induced Depression Model Mice.","authors":"Ying Wang, Guofeng Shi, Yixi Zeng, Juting Li, Yongyu Wu, Jiahui Zheng, Anjing Xu, Yanqing Ma, Lanyue Zhang, Hui Li","doi":"10.4014/jmb.2405.05013","DOIUrl":"10.4014/jmb.2405.05013","url":null,"abstract":"The disturbance of brain biochemical substances serves as a primary cause and aggravating factor of depression. This study aimed to investigate the principal components of Picea mariana and its effect on reserpine-induced depression mice,w ith its relationship with brain central transmitters and related proteins. The main constituents of P. mariana essential oil (PMEO) were analyzed by GC-MS spectrometry. The quiescent time in the tail suspension test (TST) and forced swim test (FST), along with the weight change of the mice was detected. The number of normal neurons was quantified through Nissl staining. Immunohistochemistry was employed to determine the levels of 5HT-1A and 5HT-2A in the brain. Western blotting was utilized to detect 5HT-2A, CRF and TrkB protein levels. RT-qPCR was used to detect the mRNA levels of 5HT-1A, 5HT-2A, TrkB, CRF, and BDNF. The main active ingredients of PMEOs were (-) -bornyl acetate (44.95%), γ-Terpinene (14.17%), and β-Pinene (10.12%). PMEOs effectively improved the retardation and weight loss due to anorexia in depression-like mice. This improvement was associated with an increase in the number of normal neurons. After administering different doses of PMEOs, the levels of 5HT-1A, 5HT-2A, CRF, and TrkB were found to be increased in brain tissue. RT-qPCR revealed that the mRNA levels of CRF, 5HT-1A, and 5HT-2A were generally upregulated, whereas TrkB and BDNF were downregulated. PMEO can effectively alleviate depression induced by reserpine, which may be attributed to its regulation of 5HT-1A, 5HT-2A, CRF and TrkB protein expression, thus reducing brain nerve injury.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutagenicity and Genotoxicity Assessment of Leuconostoc lactis DMLL10 Isolated from Kimchi. 从泡菜中分离出的乳酸白球菌 DMLL10 的突变性和遗传毒性评估

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-08-05 DOI: 10.4014/jmb.2405.05025

Heejung Park, Seoyeon Lee, Sojeong Heo, Do-Won Jeong

{"title":"Mutagenicity and Genotoxicity Assessment of Leuconostoc lactis DMLL10 Isolated from Kimchi.","authors":"Heejung Park, Seoyeon Lee, Sojeong Heo, Do-Won Jeong","doi":"10.4014/jmb.2405.05025","DOIUrl":"10.4014/jmb.2405.05025","url":null,"abstract":"Leuconostoc lactis DMLL10 is a microorganism specific to kimchi fermentation. In this study, we sought to evaluate the toxicity of this strain, which was newly isolated from kimchi, to determine its safety as a food ingredient. Bacterial reverse mutation assay, chromosomal aberration assay, and mammalian cell in vitro micronucleus assay were performed to assess the genetic toxicity of Leu. lactis DMLL10. The strain did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537, or Escherichia coli WP2uvrA, with or without metabolic activation of S9 mixture. The oral administration of Leu. lactis DMLL10 also did not significantly increase the number of micronucleated polychromatic erythrocytes, or the mean ratio of polychromatic to total erythrocytes. Additionally, Leu. lactis DMLL10 did not cause a significant chromosomal aberration in CHU/IL cells in the presence or absence of S9 activation. Therefore, Leu. lactis DMLL10 can be suggested as a functional food ingredient with reliability and safety.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing Strain-Specific Simple Sequence Repeat (SSR) Markers for Chlorella sorokiniana. 为小球藻开发菌株特异性简单序列重复 (SSR) 标记。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-18 DOI: 10.4014/jmb.2404.04024

Mais Sweiss, Maen Hasan, Nidal Odat

{"title":"Developing Strain-Specific Simple Sequence Repeat (SSR) Markers for Chlorella sorokiniana.","authors":"Mais Sweiss, Maen Hasan, Nidal Odat","doi":"10.4014/jmb.2404.04024","DOIUrl":"10.4014/jmb.2404.04024","url":null,"abstract":"Chlorella sorokiniana green microalga offers many environmentally friendly applications, including wastewater treatment, biofertilizers, animal feed, and biofuel production. Different strains of C. sorokiniana have unique properties that may suit one application but not another. There is a need to distinguish between the many available strains of C. sorokiniana to choose the one that best fits the application. Consequently, our research goal was to develop strain-specific simple sequence repeat (SSR) markers to differentiate between the different strains. Seventeen markers spanning ten out of the twelve chromosomes of the C. sorokiniana genome were developed and validated on eight different strains from culture collections and our lab, and were then analyzed by fragment analysis. The results demonstrate the potential of these polymorphic markers to detect the genetic differences between the strains of C. sorokiniana, and to serve as useful tools for the intra-species population genetic analysis and conservation genetics studies of C. sorokiniana.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Metal and Metal Oxide Nanoparticles against Biofilm-Forming Bacteria: A Systematic Review. 金属和金属氧化物纳米粒子对生物膜形成细菌的影响：系统综述。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-15 DOI: 10.4014/jmb.2403.03029

Hend Algadi, Mohammed Abdelfatah Alhoot, Anis Rageh Al-Maleki, Neny Purwitasari

{"title":"Effects of Metal and Metal Oxide Nanoparticles against Biofilm-Forming Bacteria: A Systematic Review.","authors":"Hend Algadi, Mohammed Abdelfatah Alhoot, Anis Rageh Al-Maleki, Neny Purwitasari","doi":"10.4014/jmb.2403.03029","DOIUrl":"10.4014/jmb.2403.03029","url":null,"abstract":"Biofilm formation by bacteria poses a significant challenge across diverse industries, displaying resilience against conventional antimicrobial agents. Nanoparticles emerge as a promising alternative for addressing biofilm-related issues. This review aims to assess the efficacy of metal and metal oxide nanoparticles in inhibiting or disrupting biofilm formation by various bacterial species. It delineates trends, identifies gaps, and outlines avenues for future research, emphasizing best practices and optimal nanoparticles for biofilm prevention and eradication. Additionally, it underscores the potential of nanoparticles as substitutes for traditional antibiotics in healthcare and combating antibiotic resistance. A systematic literature search, encompassing Web of Science, PubMed, and Google Scholar from 2015 to 2023, yielded 48 publications meeting the review criteria. These studies employed diverse methods to explore the antibacterial activity of nanoparticles against biofilm-forming bacteria strains. The implications of this study are profound, offering prospects for novel antimicrobial agents targeting biofilm-forming bacteria, often resistant to conventional antibiotics. In conclusion, nanoparticles present a promising frontier in countering biofilm-forming bacteria. This review delivers a structured analysis of current research, providing insights into the potential and challenges of nanoparticle utilization against biofilm-related challenges. While nanoparticles exhibit inherent antimicrobial properties with applications spanning healthcare, agriculture, and industries, the review acknowledges limitations such as the narrow scope of tested nanoparticles and the imperative need for extensive research on long-term toxicity and environmental impacts.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of Recombinant Human Papillomavirus 16 L1 Protein for Incorporation with para-Azido-L-Phenylalanine. 重组人乳头瘤病毒 16 L1 蛋白与对位叠氮-L-苯丙氨酸的结合工程。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-08-09 DOI: 10.4014/jmb.2407.07033

Jinhyeon Kim, Ki Jun Jeong, Geun-Joong Kim, Jong-Il Choi

引用次数: 0

Kraft Lignin Decomposition by Forest Soil Bacterium Pseudomonas kribbensis CHA-19. 森林土壤假单胞菌 CHA-19 分解牛皮纸木质素。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-19 DOI: 10.4014/jmb.2406.06021

Dockyu Kim, Han-Woo Kim, Hyoungseok Lee

{"title":"Kraft Lignin Decomposition by Forest Soil Bacterium Pseudomonas kribbensis CHA-19.","authors":"Dockyu Kim, Han-Woo Kim, Hyoungseok Lee","doi":"10.4014/jmb.2406.06021","DOIUrl":"10.4014/jmb.2406.06021","url":null,"abstract":"Identification of the biochemical metabolic pathway for lignin decomposition and the responsible degradative enzymes is needed for the effective biotechnological valorization of lignin to renewable chemical products. In this study, we investigated the decomposition of kraft lignin by the soil bacterium Pseudomonas kribbensis CHA-19, a strain that can utilize kraft lignin and its main degradation metabolite, vanillic acid, as growth substrates. Gel permeation chromatography revealed that CHA-19 decomposed polymeric lignin and degraded dehydrodivanillin (a representative lignin model compound); however, the degradative enzyme(s) and mechanism were not identified. Quantitative polymerase chain reaction with mRNAs from CHA-19 cells induced in the presence of lignin showed that the putative genes coding for two laccase-like multicopper oxidases (LMCOs) and three dye-decolorizing peroxidases (DyPs) were upregulated by 2.0- to 7.9-fold compared with glucose-induced cells, which indicates possible cooperation with multiple enzymes for lignin decomposition. Computational homology analysis of the protein sequences of LMCOs and DyPs also predicted their roles in lignin decomposition. Based on the above data, CHA-19 appears to initiate oxidative lignin decomposition using multifunctional LMCOs and DyPs, producing smaller metabolites such as vanillic acid, which is further degraded via ortho- and meta-ring cleavage pathways. This study not only helps to better understand the role of bacteria in lignin decomposition and thus in terrestrial ecosystems, but also expands the biocatalytic toolbox with new bacterial cells and their degradative enzymes for lignin valorization.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of Endophytic Fungi Isolated from Mangifera indica L. in Promoting Plant Growth. 从 Mangifera indica L. 分离的内生真菌在促进植物生长中的作用

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-19 DOI: 10.4014/jmb.2401.01034

Kanyapat Sonsiam, Orlavanh Sonesouphap, Anyaporn Sangkaew, Pariyanuj Chulaka, Prakitsin Sihanonth, Chulee Yompakdee

{"title":"Roles of Endophytic Fungi Isolated from Mangifera indica L. in Promoting Plant Growth.","authors":"Kanyapat Sonsiam, Orlavanh Sonesouphap, Anyaporn Sangkaew, Pariyanuj Chulaka, Prakitsin Sihanonth, Chulee Yompakdee","doi":"10.4014/jmb.2401.01034","DOIUrl":"10.4014/jmb.2401.01034","url":null,"abstract":"Endophytic fungi have been shown to synthesize bioactive secondary metabolites, some of which promote plant growth through various mechanisms. In our previous study, endophytic fungi were isolated from mango trees (Mangifera indica L.). The present study examined fifty endophytic fungal isolates for mineral solubilization activity, ammonia production, and siderophore production. It was shown that these isolates could produce phytohormones indole-3-acetic acid and gibberellic acid, as well as inhibit plant pathogens, specifically Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The results showed that all the isolated fungal endophytes exhibited various activities. Based on the findings, two fungal endophytes-Aureobasidium pullulans CY.OS 13 and Aspergillus tamarii CY.OS 144-were selected for dual inoculation in chili plants under pot-scale conditions to investigate their potential to improve growth-related traits such as seed germination, shoot and root length, biomass, and chlorophyll content. Seed treated with A. pullulans CY.OS 13 and/or A. tamarii CY.OS 144 showed a significant (p < 0.05) increase in seed germination and growth parameters of chili plants grown under pot-scale conditions. Particularly, chili plants whose seeds were injected with a combination of the two selected endophytic fungi showed the highest plant development traits. Therefore, the selected endophytic fungi have the potential to be used as biofertilizers, especially when combined. They could eventually replace chemical fertilizers because they are environmentally friendly, beneficial to humans, and can even promote sustainable agriculture.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0