Journal of microbiology and biotechnology最新文献

{"title":"Development of Simple Sequence Repeat (SSR) Markers from a Genome Survey of a <i>Cymbidium kanran</i> Makino Population in Jeju Island, Republic of Korea.","authors":"Kyeoung Cheol Kim, Seungtae Kang, Su-Lim Kim, Rambukkana Maggonage Thiruni Dananjana Perera, Jin Kyu Woo, Kumarasinghe Hiruni Sandunika, Ji-Hyang Kim, Dong-Sun Lee","doi":"10.4014/jmb.2501.01013","DOIUrl":"https://doi.org/10.4014/jmb.2501.01013","url":null,"abstract":"<p><p>The <i>Cymbidium kanran</i> Makino, an economically significant ornamental plant, is observed in small numbers in its natural habitat on Jeju Island in South Korea. <i>C. kanran</i> of Jeju is afforded protection due to a decline in its population resulting from environmental changes and illegal poaching. We developed simple sequence repeat (SSR) markers to analyze the differences to other <i>C. kanran</i> through molecular genetic studies. Based on the results of the Random amplified polymorphic DNA (RAPD) analysis and whole genome sequencing, 86 initial SSR marker candidates were selected in silico. After excluding those that were structurally unsuitable, 40 were reselected through polymorphism testing. Finally, 25 markers were selected based on the diversity test and their applicability to real samples. The newly developed markers will prove invaluable in substantiating the distinctiveness of <i>C. kanran</i> from Jeju, as well as in the processes of conservation, restoration, and the identification of cultivars.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501013"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Meta-Analysis on the Immunogenicity of Homologous versus Heterologous Immunization Regimens against SARS-CoV-2 Beta, Delta, and Omicron BA.1 VoCs in Healthy Adults.","authors":"Jo-Lewis Banga Ndzouboukou, Abdul A Kamara, Nadeem Ullah, Qing Lei, Xiong-Lin Fan","doi":"10.4014/jmb.2411.11059","DOIUrl":"https://doi.org/10.4014/jmb.2411.11059","url":null,"abstract":"<p><p>Since the outbreak of the COVID-19 pandemic, SARS-CoV-2 has not stopped evolving, leading to the emergence of variants of concern (VoCs) involved in significant immune escape. Here, we compared the immunogenicity of different prime-boost vaccination regimens against SARS-CoV-2 wildtype (WT) and its Beta, Delta, and Omicron BA.1 VoCs. We used 5 databases to retrieve publications and random-effect models to estimate pooled neutralization titers. We included 11 randomized controlled trials (RCTs) and 16 non-RCTs, 10 prime-boost vaccination regimens, and 4598 subjects. We found neutralization activity against SARS-CoV-2 decreased with virus evolution. The heterologous immunization was more effective. The increase in neutralization titers against SARS-CoV-2 WT and Beta, Delta, and Omicron BA.1 VoCs after heterologous immunization was 1.41(95%CI:0.82-2.01), 0.90(95%CI:0.39-1.41), 1.23 (95%CI: 0.81-1.65), and 1.32 (95%CI: 0.99-1.65), respectively. Furthermore, the booster dose of viral vector vaccine did not show a higher increase in neutralization titers against SARS-CoV-2 WT(MD=0.48; 95%CI:-1.12-1.09), Beta (MD=0.20; 95%CI:-0.26-0.67), Delta (MD=0.35; 95%CI:-0.09-0.79), and Omicron BA.1 (MD=0.38; 95%CI:-0.14-0.89) VoCs. The combination of inactivated-recombinant protein vaccines showed a higher increase in neutralization titers (Beta: MD=1.88 and Delta: MD=1.70) than other combinations of vaccines. However, only a combination of mRNA-viral vector vaccines showed a higher increase in neutralization titers (MD:1.52; 95%CI:0.34-2.70) against Omicron BA.1 VoC. Interestingly, the viral vector-mRNA immunization regimen appears better compared to mRNA-viral vector regimen, especially against Beta and Delta VoCs. Overall, the type of combination followed by the order of administration of COVID-19 vaccines could be a potential vaccine strategy against the occurrence of SARS-CoV-2 variants.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411059"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broad Antibacterial Activity and Mechanism of Garlic (<i>Allium sativum</i> L. cv. Uiseong) Extracts against Cell Wall of <i>Aeromonas hydrophila</i>.","authors":"Putri Christy Artawinata, Youjin Kim, In Young Choi, Mi-Kyung Park","doi":"10.4014/jmb.2410.10035","DOIUrl":"10.4014/jmb.2410.10035","url":null,"abstract":"<p><p><i>Aeromonas hydrophila</i> is a pathogenic bacterium known for its resistance to antibiotics and its ability to cause infections in aquatic environments. This has made disease management more complex, making the development of alternative antimicrobial agents necessary. Uiseong garlic is a superior variety from Republic of Korea, renowned for its high level of beneficial compounds, making it a promising candidate for natural antimicrobial application. Therefore, this study aimed to investigate the antibacterial activity and mechanism of Uiseong garlic extracts against the cell wall of <i>A. hydrophila</i>. Uiseong garlic extracts were prepared using water and ethanol at 22°C and 90°C, respectively. The antibacterial activities of Uiseong garlic extracts were evaluated for their yield, antibacterial dynamics, leakage of bacterial intracellular proteins, and changes in morphological characteristics. Uiseong garlic extracts at 22°C exhibited significant antibacterial activities against foodborne pathogens, particularly against 9 strains of <i>A. hydrophila</i>. In this study, the ethanol extract at 22°C demonstrated significantly higher antibacterial activity compared to the water extract at 22°C, with a similar pattern of antimicrobial dynamics with polymyxin B. The ethanol extract at 22°C caused a higher concentration of leaked bacterial proteins (92.87 ± 0.46 μg/ml), indicating cell membrane disruption. Additionally, transmission electron microscopy analysis further confirmed that both extracts induced plasmolysis, leading to notable damage to the bacterial cell membrane. Therefore, ethanol extract of Uiseong garlic was demonstrated as a promising alternative to antibiotics for controlling <i>A. hydrophila</i>.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2410035"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-Dependent Responses of <i>Escherichia coli</i> and <i>Acinetobacter</i> sp. to Micron-Sized Polystyrene Microplastics.","authors":"So Yoon Kim, Shinyoung Woo, Seung-Woo Lee, Eui-Man Jung, Eun-Hee Lee","doi":"10.4014/jmb.2410.10023","DOIUrl":"10.4014/jmb.2410.10023","url":null,"abstract":"<p><p>Microplastics are ubiquitous environmental contaminants that can cause significant ecological damage because of their resistance to biodegradation. We evaluated the toxic effects of 1,040 nm polystyrene (PS) microplastics in two representative bacteria, <i>Escherichia coli</i> and <i>Acinetobacter</i> sp. In particular, we examined the effects of these PS microplastics on bacterial growth and viability, parameters related to oxidative stress (reactive oxygen species [ROS], lactate dehydrogenase [LDH], and malondialdehyde [MDA]), membrane integrity, and biofilm formation. An increasing concentration of PS microplastics decreased cell growth and viability in both species. These PS microplastics also decreased cell membrane integrity and increased biofilm formation in both species. Although both species exhibited adverse overall effects from PS microplastics, they had significant differences in specific indicators of oxidative stress. Correlation analysis demonstrated different correlations among measured experimental parameters (cell viability, ROS, LDH, MDA, and biofilm formation) in these two species. These results suggest that 1,040 nm PS microplastics decreased cell growth and viability by different mechanisms in <i>E. coli</i> and <i>Acinetobacter</i> sp.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2410023"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbonatogenic Bacteria in the Maros-Pangkep Karst: Protectors or Threat to Prehistoric Paintings?","authors":"Nur Haedar, Faisal, Nur Afifah Zhafirah, Riuh Wardhani, Asadi Abdullah, Rustan Lebe, Fuad Gani, Heriadi","doi":"10.4014/jmb.2410.10019","DOIUrl":"10.4014/jmb.2410.10019","url":null,"abstract":"<p><p>The Maros-Pangkep karst region hosts prehistoric cave paintings recognized by UNESCO as a world cultural heritage site. The presence of calcium carbonate (CaCO₃) on the surface of these artworks suggests the involvement of carbonatogenic bacteria, which facilitate CaCO₃ production or deposition. While these bacteria have been explored for their potential in stone artwork conservation, their role in either preserving or obscuring prehistoric paintings remains unclear. This study aims to identify carbonatogenic bacteria associated with the Maros-Pangkep cave paintings and evaluate their CaCO₃ precipitation potential. Bacteria were isolated using Calcium Carbonate Precipitation (CCP) medium, and their CaCO₃ precipitation capacity was assessed by measuring precipitate mass and ammonia (NH₃) levels. Molecular identification was conducted using 16S rRNA gene sequencing. Eighteen bacterial isolates were obtained from swab samples collected in Parewe and Bulu Sipong caves, ten of which were identified as carbonatogenic. Among these, two isolates exhibited the highest CaCO₃ precipitation: Ps1-d produced 2.45 ± 0.07 mg/ml CaCO₃ with 946.3 ± 26.3 mg/l NH₃, while Ps8-b produced 1.80 ± 0.05 mg/ml CaCO₃ with 763.4 ± 21.2 mg/l NH₃. Molecular analysis identified Ps1-d as <i>Bacillus</i> cereus strain bk and Ps8-b as <i>Bacillus</i> sp. NCCP-428. These findings have significant implications for both (1) the potential application of carbonatogenic bacteria in the conservation and restoration of stone artworks and (2) the development of strategies to inhibit excessive CaCO₃ deposition to prevent the obscuration of cultural heritage paintings.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2410019"},"PeriodicalIF":2.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and Mechanism of <i>Tricholoma matsutake</i> Extract on UVA and UVB Radiation-Induced Skin Aging.","authors":"Lu Hu, Zhenhai Huang, Jiyu Weng, Chujie Huang, Lanyue Zhang","doi":"10.4014/jmb.2411.11085","DOIUrl":"https://doi.org/10.4014/jmb.2411.11085","url":null,"abstract":"<p><p>Ultraviolet (UV) radiation often causes skin aging, inflammation, cancer and other related skin diseases. In this study, the main components of <i>Tricholoma matsutake</i> extract (TME) were identified using UPLC-Q-TOF-MS, and their anti-photoaging effects were assessed through UV-induced cell and animal models. The key components identified were D-mannitol (27.41%), DL-malic acid (14%), alginate (12.5%), isoleucine (4.82%), and phenylalanine (4.31%), all of which played roles in anti-aging and UV protection. TME (50-100 mg/ml) significantly alleviated UVA/UVB-induced erythema and wrinkles in mice. Pathological staining showed that TME suppressed UV-induced epidermal hyperplasia (<i>p</i> < 0.05), reduced collagen damage (<i>p</i> < 0.01), and decreased mast cell infiltration (<i>p</i> < 0.01), while down-regulating inflammatory markers such as IL-6, IL-1β, and TNF-α. TME also upregulated type I collagen (COL-1). Flow cytometry results demonstrated that high-dose TME inhibited UV-induced apoptosis and reduced reactive oxygen species (ROS) in HaCaT cells (<i>p</i> < 0.05). Immunofluorescence and scratch migration assays showed that TME promoted PPAR-α expression, reduced inflammation, and supported skin repair (<i>p</i> < 0.01). Transcriptomic and metabolomic analyses indicated that TME regulated inflammation-related signaling pathways, helping to prevent skin aging. TME is a promising natural product for skin care and treatment of oxidative stress and inflammation-related diseases.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411085"},"PeriodicalIF":2.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> and <i>In Silico</i> Antimicrobial Activity against Methicillin-Resistant <i>Staphylococcus aureus</i> of Essential Oils from Four Medicinal Plants in Xuan Thuy Mangrove Forest.","authors":"Ngoc Anh Luu Dam, Van Huong Bui, Ton That Huu Dat, Khac Tiep Nguyen, Thanh Tung Nguyen, Quang Quy Duong, Tung Lam Vo, Vladimir V Titok","doi":"10.4014/jmb.2409.09006","DOIUrl":"10.4014/jmb.2409.09006","url":null,"abstract":"<p><p><i>Sphagneticola trilobata</i>, <i>Vitex rotundifolia</i>, <i>Vitex trifolia</i>, and <i>Annona glabra</i> are medicinal plants found in Xuan Thuy mangrove forest that contain essential oils (EOs) and the potential to treat infections. The obtained yields of hydrodistillation essential oil from aerial parts and leaves ranged from 0.01% to 0.12% (v/w) on a fresh-weight basis. Subsequently, <i>in vitro</i> assessments indicated that the EOs of <i>A. glabra</i>, <i>S. trilobata</i>, and <i>V. trifolia</i> displayed antibacterial effects against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), with <i>S. trilobata</i> exhibiting bactericidal effects at minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.2% EO, and <i>A. glabra</i> exhibiting bacteriostatic effects with both MIC and MBC values of 0.8% EO. However, the EO of <i>V. rotundifolia</i> showed no inhibitory effects against <i>S. aureus</i>, even at concentrations of up to 0.8% EO. Furthermore, docking results showed that six compounds exhibited particularly high binding energies (< -7 kcal/mol): <i>α</i>-phellandrene, <i>β</i>-caryophyllene, <i>β</i>-guaiene, alloaromadendrene, bicyclosesquiphellandrene, and bicyclogermacrene. Among these, <i>α</i>-phellandrene, <i>β</i>-caryophyllene, bicyclosesquiphellandrene, and bicyclogermacrene are the main components in the <i>S. trilobata</i> EO; <i>β</i>-caryophyllene, <i>β</i>-guaiene, and alloaromadendrene are the main components in the <i>A. glabra</i> EO; and <i>β</i>-caryophyllene and alloaromadendrene are the main components in the <i>V. trifolia</i> EO. These findings highlight the antibacterial potential of EOs from mangrove plants; therefore, expanded investigations into EOs and the biological properties of mangrove plants may be a promising strategy for combating antimicrobial resistance.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2409006"},"PeriodicalIF":2.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of Acute Ulcerative Colitis with Zinc Hyaluronate in Mice.","authors":"Lan Zhang, Xuedan Fu, Jiazheng Li, Wan Xiao, Xi Xiong, Huixia Lv, Zhenhai Zhang, Jianming Ju","doi":"10.4014/jmb.2408.08050","DOIUrl":"10.4014/jmb.2408.08050","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is a type of inflammatory bowel disease arising from numerous factors, while UC patients face insufficient treatment options and a high incidence of adverse reactions to the current therapies. As a functional food additive, hyaluronic acid plays a certain role in intestinal repair. In this study, we constructed a mouse model of dextran sulfate sodium (DSS)-induced UC to examine the effects and underlying mechanisms of action of zinc hyaluronate (ZnHA) on the pathogenesis of UC. ZnHA effectively alleviated key clinical UC symptoms, such as weight loss, loose stools, and bloody stools. Mechanistically, ZnHA attenuated the expression of inflammatory factors, such as tumor necrosis factor-α, interleukin (IL)-6, and myeloperoxidase while upregulating the expression of IL-10. Furthermore, through intestinal flora and short-chain fatty acid analyses, ZnHA was found to promote propionic acid production by enriching beneficial bacteria. ZnHA simultaneously enhanced the expression of tight junction proteins, specifically ZO-1 and occludin, thereby restoring intestinal barrier function. Overall, our findings elucidate the therapeutic potential of ZnHA in treating acute UC by inhibiting intestinal inflammation and regulating flora, while also providing further theoretical support for development of hyaluronic acid to treat this disease.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2408050"},"PeriodicalIF":2.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Blue Light Receptors LreA and LreB in <i>Aspergillus flavus</i>.","authors":"Hye-Min Park, Ye-Eun Son, He-Jin Cho, Jae-Hyuk Yu, Hee-Soo Park","doi":"10.4014/jmb.2411.11054","DOIUrl":"10.4014/jmb.2411.11054","url":null,"abstract":"<p><p>Light is a key external signal factor that regulates asexual development, stress resistance, and secondary metabolism in fungi. In the presence of light, photoreceptors sense several light receptors and affect fungal life. In this study, we characterized the function of the blue light receptors LreA and LreB in <i>Aspergillus flavus</i>, a potent pathogenic and toxigenic fungus. <i>lreA</i> or <i>lreB</i> deletion increased the growth rate but decreased conidial production in the presence or absence of light. The Δ<i>lreA</i>-mutant strain and the Δ<i>lreB</i>-mutant strain produced abnormal conidiophores, suggesting that <i>lreA</i> and <i>lreB</i> were essential for proper conidiation in <i>A. flavus</i>. The absence of <i>lreA</i> or <i>lreB</i> slightly decreased the stress response tolerance against thermal and oxidative stresses. In kernel infection, the Δ<i>lreA</i> mutant strain and the Δ<i>lreB</i> mutant strain produced conidia and aflatoxin B1 that were less than those produced by the control strains. Therefore, LreA and LreB play key roles in the growth, asexual development, and pathogenicity of <i>A. flavus</i>.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411054"},"PeriodicalIF":2.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol and Growth Promotion of Rice by <i>Pseudomonas aeruginosa</i> SNTKU16: Beneficial Properties and Genomic Potential.","authors":"Sutticha Na-Ranong Thammasittirong, Anon Thammasittirong, Sukanya Saechow","doi":"10.4014/jmb.2411.11067","DOIUrl":"10.4014/jmb.2411.11067","url":null,"abstract":"<p><p>Dirty panicle and sheath blight are important diseases that have the capacity to reduce rice productivity and grain quality. The bacterial strain SNTKU16 was isolated from soil in a sugarcane field and identified as <i>Pseudomonas aeruginosa</i>. This bacterium and its cell-free culture exhibited strong fungal antagonistic activity against a range of rice dirty panicle pathogens (<i>Curvularia lunata</i>, <i>Fusarium semitectum</i>, and <i>Helminthosporium oryzae</i>) and a sheath blight pathogen (<i>Rhizoctonia solani</i>). In addition to its role in disease control, the cell suspension and cell-free culture of this strain significantly enhanced rice seed germination and seedling growth. Furthermore, this bacterium exhibited various plant growth-promoting traits such as indole acetic acid, ammonia and siderophore productions, and phosphate solubilization. Genome analysis of SNTKU16 revealed its genetic potential for controlling plant diseases through direct antagonistic activities against pathogens, as well as indirect mechanisms, such as promoting plant growth. These capabilities suggest a multifaceted approach to disease management and plant growth promotion, making this strain an intriguing candidate for further exploration in sustainable agriculture and microbial biotechnology.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411067"},"PeriodicalIF":2.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}