Journal of microbiology and biotechnology最新文献_第4页

Simple and Sensitive Escherichia coli Analysis via Allosteric Probe Controllable Switch Cas12a/crRNA Complex Mediated Strategy. 变构探针可控开关Cas12a/crRNA复合物介导的简单灵敏大肠杆菌分析

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-28 DOI: 10.4014/jmb.2506.06010

Yan Jiang, Chunling Zhao, Xiaoxia Fang, Xinning Shi, Hongyang Qi

{"title":"Simple and Sensitive Escherichia coli Analysis via Allosteric Probe Controllable Switch Cas12a/crRNA Complex Mediated Strategy.","authors":"Yan Jiang, Chunling Zhao, Xiaoxia Fang, Xinning Shi, Hongyang Qi","doi":"10.4014/jmb.2506.06010","DOIUrl":"10.4014/jmb.2506.06010","url":null,"abstract":"The development of an innovative, portable, and cost-effective biosensor for rapid and accurate bacterial detection represents a significant advancement over conventional methods, offering a promising diagnostic tool for infection control in clinical nursing. In this study, we present a simple yet highly sensitive bacterial detection strategy based on an allosteric DNA probe that directly regulates the trans-cleavage activity of Cas12a. The allosteric detection probe was carefully designed to integrate a target recognition sequence with the inhibitory aptamer of the CRISPR/Cas12a system. Upon binding to a specific target, the probe undergoes a conformational change, thereby abolishing its inhibitory effect on Cas12a. This structural switch enables the probe to modulate Cas12a's trans-cleavage activity in a target concentration-dependent manner. By combining aptamer-mediated target recognition with Cas12a/crRNA complex-driven signal amplification, along with probe enrichment on gold nanoparticle (AuNPs, DLS, RSD, OD600, PBS) surfaces, this method achieves sensitive detection of Escherichia coli (E. coli). The assay demonstrates a detection limit of 4.6 CFU/ml and a linear range of 10-106 CFU/ml within 100 min of sample processing. Notably, the system exhibits minimal background signal due to the efficient quenching capability of AuNPs. Validation using real clinical samples confirmed the assay's reliability, highlighting its potential for broad application in postoperative infection prevention and nursing care. Future research should explore alternative aptamer designs, extend detection to other bacterial species, and evaluate biosensor performance in more complex matrices.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506010"},"PeriodicalIF":3.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Multi-Omics Analysis and Antitumor Activity of Phylloporia crataegi and Phylloporia fontanesiae. 山竹和山竹的多组学比较及抗肿瘤活性研究。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-28 DOI: 10.4014/jmb.2504.04029

Yan Fu, Yuyue Zhang, Zhihao Guo, Guoli Zhang, Yinghao Zhang, Xuemei Tian

{"title":"Comparative Multi-Omics Analysis and Antitumor Activity of Phylloporia crataegi and Phylloporia fontanesiae.","authors":"Yan Fu, Yuyue Zhang, Zhihao Guo, Guoli Zhang, Yinghao Zhang, Xuemei Tian","doi":"10.4014/jmb.2504.04029","DOIUrl":"10.4014/jmb.2504.04029","url":null,"abstract":"Wood-decay fungi, particularly those within the Phylloporia genus, have attracted considerable interest due to their diverse bioactivities, including antitumor, immune-modulatory, anti-inflammatory, antiviral, antioxidant, and antibacterial effects. This study conducted a comparative analysis of the antitumor activities and underlying molecular mechanisms of two Phylloporia species, Phylloporia crataegi and Phylloporia fontanesiae, from Shandong Province. In vitro assays demonstrated that ethanol extract from the two species exhibited significant, time- and concentration-dependent inhibition of breast cancer (MCF-7), esophageal cancer (Eca-109), and human non-small cell lung cancer (A549). Notably, the ethanol extract of P. crataegi (PCCT) displayed the most potent antitumor activity, with the highest inhibition of Eca-109 cells, achieving an IC50 of 1.310 ± 0.052 mg/ml. Metabolomic analysis revealed that P. crataegi contained significantly higher levels of antitumor-associated metabolites, including trans-cinnamic acid, AMP, and 6-phospho-α-D-glucan (P < 0.05). Transcriptomic analysis further indicated that genes involved in cellular stress responses and metabolic pathways were significantly upregulated in P. crataegi, including IRE1-mediated unfolded protein responses, ATPase activity, and calcium signaling pathways, contributing to its antitumor properties. Proteomic analysis corroborated these findings, identifying differentially expressed proteins predominantly associated with secondary metabolite biosynthesis and energy metabolism. This study elucidates the molecular mechanisms underlying the superior antitumor activity of P. crataegi, underscoring the potential of Phylloporia species as valuable resources for drug discovery and therapeutic development.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504029"},"PeriodicalIF":3.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplement C18:1 in Culture Medium to Improve Survival Rate and Fermentation Activity of Lyophilization of Lacticaseibacillus paracasei L9. 在培养基中添加C18:1提高副干酪乳杆菌L9的存活率和冻干发酵活性

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-28 DOI: 10.4014/jmb.2505.05007

Weina Miao, Li Lin, Yanling Hao, Zhengyuan Zhai, Ran Wang, Jingjing Liang, Ming Zhang, Yue Sang, Hongliang Li, Lianzhong Ai, Liang Zhao

{"title":"Supplement C18:1 in Culture Medium to Improve Survival Rate and Fermentation Activity of Lyophilization of Lacticaseibacillus paracasei L9.","authors":"Weina Miao, Li Lin, Yanling Hao, Zhengyuan Zhai, Ran Wang, Jingjing Liang, Ming Zhang, Yue Sang, Hongliang Li, Lianzhong Ai, Liang Zhao","doi":"10.4014/jmb.2505.05007","DOIUrl":"10.4014/jmb.2505.05007","url":null,"abstract":"This study explored the impact of C18:1 fatty acid supplementation in culture medium on the survival rate, fermentation activity, and membrane stability of Lacticaseibacillus paracasei L9 during freeze-drying. Cells cultured in medium supplemented with C18:1 (0.2 mg/ml) exhibited a higher survival rate (95.38%) after freeze-drying compared to the control (83.56%). The fermentation activity of the freeze-dried powder was significantly enhanced, with improvements in viable count (Δ viable counts: 1.79 Log CFU/ml), pH reduction (Δ pH: 2.03), and titratable acidity (Δ titratable acidity: 99.4 °T) in skim milk. Supplementation with C18:1 increased the activity of key enzymes, such as β-galactosidase (13.25 U/ml) and lactate dehydrogenase (2.16 U), while reducing cell membrane permeability. The addition of C18:1 also modulated the expression of membrane synthesis-related genes, including plsX and YfhO, and altered the membrane fatty acid composition by increasing the proportion of unsaturated fatty acids (UFA). Specifically, the proportion of UFA in the membrane increased from 53.36% in the freeze-dried control to 63.28% in the C18:1-supplemented group. These results indicate that C18:1 supplementation improves cell membrane stability and fermentation activity by modulating gene expression and membrane fatty acid composition, thereby enhancing the freeze-drying tolerance of L. paracasei L9.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505007"},"PeriodicalIF":3.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Basis for Tetramerization of Klebsiella pneumoniae N-Acetylglucosamine-6-Phosphate Deacetylase. 肺炎克雷伯菌n -乙酰氨基葡萄糖-6-磷酸脱乙酰酶四聚化的结构基础。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-26 DOI: 10.4014/jmb.2505.05019

So Yeon Lee, Hyun Ho Park

引用次数: 0

Distinct Microbial Signatures along the Female Reproductive Tract in Endometrial Cancer Patients. 子宫内膜癌患者生殖道中不同的微生物特征。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-26 DOI: 10.4014/jmb.2503.03048

Weicong Xu, Ayana Bayijuma, Jie Shi, Yujie Huang, Jingyu Wang, Kaiyou Fu, Baohong Wang, Yunxiao Zhou

{"title":"Distinct Microbial Signatures along the Female Reproductive Tract in Endometrial Cancer Patients.","authors":"Weicong Xu, Ayana Bayijuma, Jie Shi, Yujie Huang, Jingyu Wang, Kaiyou Fu, Baohong Wang, Yunxiao Zhou","doi":"10.4014/jmb.2503.03048","DOIUrl":"10.4014/jmb.2503.03048","url":null,"abstract":"Emerging evidence suggests that microbiota dysbiosis plays a critical role in the pathogenesis of endometrial cancer (EC), a leading cause of cancer-related deaths in women globally. However, few studies have simultaneously examined both the upper and lower genital tract microbiota in such individuals. In this study, we investigated alterations in microbiota composition across different parts of the female genital tract in a Chinese cohort of EC patients. Samples from 59 individuals (22 endometrial cancer patients; 8 endometrial hyperplasia patients, and 29 benign controls) were collected. In addition, a total of 58 vaginal swabs, 39 fallopian swabs, 16 peritoneal fluid samples, 36 urine swabs, and 34 endometrium samples were finally recruited. The composition of bacterial communities was determined by 16S ribosomal RNA Miseq sequencing. Specific taxa were significantly enriched in the EC group, including Akkermansia muciniphila, Acinetobacter, and Pseudomonas in the vagina, and Pseudomonas, Bacillus, Streptomyces, and Burkholderia-Caballeronia-Paraburkholderia in the endometrium. Meanwhile, Acinetobacter was positively correlated with fasting plasma glucose, while Pseudomonas was correlated with estrogen and progesterone receptor expression. An effective random forest model enabled us to distinguish EC patients from benign controls. Moreover, specific alterations in the composition and diversity of the reproductive tract microbiota in endometrial cancer patients were identified. Our findings suggest a potential link between microbiome alterations and estrogen and glucose metabolism in EC. However, further investigation is needed to elucidate the molecular mechanisms underlying these associations.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503048"},"PeriodicalIF":3.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of High-Throughput Screening Protocol Based on Isomerase Using Geobacillus sp. L-Rhamnose Isomerase. l -鼠李糖异构酶高通量筛选方案的建立。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-26 DOI: 10.4014/jmb.2507.07026

Na Kyeong Koo, Sol Min Han, Seong-Bo Kim, Seung-Ho Baek, Hyun June Park

{"title":"Establishment of High-Throughput Screening Protocol Based on Isomerase Using Geobacillus sp. L-Rhamnose Isomerase.","authors":"Na Kyeong Koo, Sol Min Han, Seong-Bo Kim, Seung-Ho Baek, Hyun June Park","doi":"10.4014/jmb.2507.07026","DOIUrl":"10.4014/jmb.2507.07026","url":null,"abstract":"Directed evolution is a powerful tool in protein engineering that generates diverse variant libraries to enhance enzyme functionalities. However, the identification of desirable variants from large mutant libraries requires an efficient high-throughput screening (HTS) technique. In this study, we established a robust HTS protocol for selecting high-activity isomerase variants, specifically using L-rhamnose isomerase (L-RI) consistently; avoid switching between L-RI and L-RhI. L-RI catalyzes the isomerization of D-allulose to D-allose, which allows for activity detection via the reduction of the ketose D-allulose in a colorimetric assay based on Seliwanoff's reaction. Initial optimization was conducted in a single-tube format, where reaction conditions were refined and interfering factors were minimized. This optimized single-tube protocol demonstrated excellent accuracy when validated against high-performance liquid chromatography measurements, confirming its ability to precisely quantify D-allulose depletion. Subsequently, the protocol was successfully adapted to a 96-well plate format, incorporating further optimizations for protein expression and the removal of denatured enzymes. This involved methods such as cell harvest, supernatant removal, and filtration to reduce assay interference. The analytical quality of the established HTS protocol was evaluated using statistical metrics. The results yielded a Z'-factor of 0.449, a signal window (SW) of 5.288, and an assay variability ratio (AVR) of 0.551. All these values meet the acceptance criteria for high-quality of HTS assay. This HTS protocol is highly reliable and applicable for efficient screening of isomerase activity in various industrial and research areas.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507026"},"PeriodicalIF":3.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Chronic Administration of a Standardized Rice Bran Supplement on Sleep Architecture and Profiles in Mice: A Comparative Study with a Hypnotic Drug Zolpidem. 长期服用标准米糠补充剂对小鼠睡眠结构和特征的影响：与催眠药物唑吡坦的比较研究。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-26 DOI: 10.4014/jmb.2505.05010

Minseok Yoon, Sangoh Kwon, Min Young Um, Suengmok Cho

{"title":"Effects of Chronic Administration of a Standardized Rice Bran Supplement on Sleep Architecture and Profiles in Mice: A Comparative Study with a Hypnotic Drug Zolpidem.","authors":"Minseok Yoon, Sangoh Kwon, Min Young Um, Suengmok Cho","doi":"10.4014/jmb.2505.05010","DOIUrl":"10.4014/jmb.2505.05010","url":null,"abstract":"This study aimed to investigate the effects of chronic administration of rice bran supplement (RBS) on sleep and to compare these effects with those of the hypnotic drug zolpidem (ZPD). During the three-week treatment period, ZPD initially reduced sleep latency, but that reduction effect of sleep latency gradually diminished over time and significantly decreased non-rapid eye movement sleep (NREMS). In contrast, RBS reduced sleep latency and increased NREMS on the first day, and these effects persisted throughout the treatment period. Additionally, RBS altered the time course of NREMS, which persisted until day 21. Importantly, while RBS did not affect delta activity during the treatment period, the ZPD group experienced a decrease in delta activity on the first day, which recovered by day 21. Our findings suggest that RBS does not induce tolerance during chronic administration and does not alter sleep architecture upon discontinuation, indicating that it could be utilized as a natural sleep aid suitable for long-term consumption.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505010"},"PeriodicalIF":3.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila Alleviates Sarcopenia in Senescence-Accelerated Mouse-Prone 8 Mice. 嗜粘蛋白阿克曼氏菌减轻衰老加速小鼠肌肉减少症。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-26 DOI: 10.4014/jmb.2507.07001

So-Hyun Park, Hee Soo Kim, Pyeong Geun Choi, Myeong Seon Jeong, Yang Hoon Huh, Jiyun Ahn, Chang Hwa Jung

{"title":"Akkermansia muciniphila Alleviates Sarcopenia in Senescence-Accelerated Mouse-Prone 8 Mice.","authors":"So-Hyun Park, Hee Soo Kim, Pyeong Geun Choi, Myeong Seon Jeong, Yang Hoon Huh, Jiyun Ahn, Chang Hwa Jung","doi":"10.4014/jmb.2507.07001","DOIUrl":"10.4014/jmb.2507.07001","url":null,"abstract":"Akkermansia muciniphila, an intestinal bacterium, has garnered attention for its association with metabolic health and anti-inflammatory properties. However, its potential role in mitigating sarcopenia, particularly in the senescence-accelerated mouse-prone 8 (SAMP8) model, remains unexplored. In this study, we aimed to evaluate the potential effects of A. muciniphila supplementation on sarcopenia and its underlying mechanisms. Seven-month-old SAMP8 mice were administered A. muciniphila for 3 months. A. muciniphila supplementation enhanced grip strength and skeletal muscle mass, suppressed cellular senescence, improved the balance between protein degradation and synthesis, increased total ATP, and improved mitochondrial biogenesis. Analysis of the effects of A. muciniphila on gut microbiome using 16S rRNA sequencing showed that supplementation with A. muciniphila shifted the gut microbiota composition, alleviated gut dysbiosis, preserved gut barrier integrity, and reduced the protein expression of inflammatory cytokines in the intestine. Additionally, extracellular vesicles derived from A. muciniphila promoted myogenesis and suppressed dexamethasone-induced atrophy in C2C12 myoblasts. These findings suggest that supplementation with A. muciniphila mitigates sarcopenia by suppressing inflammation and improving the gut microenvironment, highlighting the potential of A. muciniphila as a new therapeutic candidate to treat sarcopenia.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2507001"},"PeriodicalIF":3.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory Effects of LSI312A on Dendritic Cells: A Novel Approach to Modulating Inflammatory Pathways. LSI312A对树突状细胞的免疫调节作用：一种调节炎症通路的新途径。

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-18 DOI: 10.4014/jmb.2506.06027

Hien Thi Thu Do, Chaelin Lee, Inmoo Rhee

{"title":"Immunomodulatory Effects of LSI312A on Dendritic Cells: A Novel Approach to Modulating Inflammatory Pathways.","authors":"Hien Thi Thu Do, Chaelin Lee, Inmoo Rhee","doi":"10.4014/jmb.2506.06027","DOIUrl":"10.4014/jmb.2506.06027","url":null,"abstract":"Inflammation plays a crucial role in the pathogenesis of various diseases, necessitating the development of effective anti-inflammatory therapeutics. Dendritic cells (DCs), as professional antigen-presenting cells, are key regulators of immune responses. In this study, we investigated the immunomodulatory effects of LSI312A, a novel compound derived from medicinal plant analogues, on DC function and inflammatory signaling pathways. LSI312A exhibited no cytotoxicity in DC2.4 cells at concentrations up to 20 μM. LSI312A significantly reduced antigen uptake and impaired the expression of co-stimulatory molecules, particularly MHC class II and CD40, upon lipopolysaccharide (LPS) stimulation. Moreover, LSI312A markedly suppressed the secretion of pro-inflammatory cytokines, including TNF-α and IL-6, and decreased nitric oxide (NO) production by downregulating iNOS expression at both the mRNA and protein levels. Mechanistically, LSI312A inhibited the phosphorylation of NF-κB, a central regulator of inflammatory responses, while promoting Nrf2 nuclear translocation, an essential factor in antioxidant signaling. Furthermore, LSI312A effectively suppressed the activation of the PI3K/Akt pathway, contributing to its anti-inflammatory effects. These results suggest that LSI312A modulates key inflammatory pathways and DC-mediated immune responses, highlighting its potential as a novel therapeutic candidate for inflammation-related diseases.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506027"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Research Progress on the Microbial or Enzymatic Degradation and Mechanism of Aflatoxin B1. 黄曲霉毒素B1的微生物或酶降解及其机理研究进展

IF 3.1 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-08-18 DOI: 10.4014/jmb.2504.04044

Aiyuan Zhang, Jiguo Yang

{"title":"A Review of Research Progress on the Microbial or Enzymatic Degradation and Mechanism of Aflatoxin B1.","authors":"Aiyuan Zhang, Jiguo Yang","doi":"10.4014/jmb.2504.04044","DOIUrl":"10.4014/jmb.2504.04044","url":null,"abstract":"Aflatoxins (AFs) are secondary metabolites produced by toxigenic Aspergillus species, which are highly toxic and exhibit carcinogenic, mutagenic, and teratogenic properties, posing a significant threat to human and animal health. Cereals and their products are the primary targets for aflatoxin-producing fungal contamination, causing substantial losses to the livestock and food industries. Due to the high toxicity and widespread contamination of aflatoxin B1 (AFB1), the prevention and degradation of AFs in cereals and their products are essential. This review summarizes the AFB1-degrading bacteria, such as Bacillus, Actinomycetes, and γ-Proteobacteria, as well as fungi, including Aspergillus and Basidiomycota. It also provides an overview and discussion of the chemical structures and toxicity of the degradation products. Additionally, the review delves into the possible oxidative, reductive, and hydrolytic mechanisms of AFB1 enzymatic degradation. The aim of this study is to provide a reference for the biological degradation of AFB1 and to promote further research in this area.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504044"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0