Journal of microbiology and biotechnology最新文献_第4页

Effects of Peptidase Treatment on Properties of Yeast Protein as an Alternative Protein Source.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-11-18 DOI: 10.4014/jmb.2409.09062

Ju Hyun Min, Nur Istianah, Jeong Hwa Jang, Hyeon Ji Jeon, Young Hoon Jung

{"title":"Effects of Peptidase Treatment on Properties of Yeast Protein as an Alternative Protein Source.","authors":"Ju Hyun Min, Nur Istianah, Jeong Hwa Jang, Hyeon Ji Jeon, Young Hoon Jung","doi":"10.4014/jmb.2409.09062","DOIUrl":"10.4014/jmb.2409.09062","url":null,"abstract":"Yeast protein, high-quality and high-content microbial protein, can serve as alternative sources of protein. This study examined the structural and functional characteristics of yeast protein through enzymatic treatment using different ratios of alcalase (endo-type) and prozyme 2000P (exo-type) including 2:1 (A2P1), 1:1 (A1P1), and 1:2 (A1P2). After enzymatic hydrolysis, a significant increase in protein solubility from less than 3.1% in untreated proteins to around 16%, particularly at pH 2 or pH 12. Furthermore, a maximum degree of hydrolysis of over 85% was achieved after enzyme treatment. Among them, the highest value of 87.73% was achieved at yeast protein treated by A1P2. Scanning electron microscopy images revealed varied surface morphologies, with exhibiting an increased surface area, particularly after treatment using A2P1. Next, yeast protein treated with A2P1 also demonstrated a superior emulsion stability index (3364.17). However, the antioxidant capacity was higher in proteins treated with A1P2 (78.30%). In addition, the elevated levels of certain amino acids, specifically leucine, lysine, phenylalanine, valine, and arginine, thereby indicating an enhanced amino acid profile was observed. Overall, yeast proteins treated with complex enzymes exhibited improved functionality and potential for diverse food applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2596-2608"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Safety and Impact of Heat-Treated Lactiplantibacillus plantarum KM2 Fermentation on Gut Microbiome Architecture.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-27 DOI: 10.4014/jmb.2411.11069

Seok Won, Yejin Jeong, Ji-Eun Kim, Jong-Hoon Kim, Hyun Soo Song, Hyun-Hwa Bae, Mi-Sun Kwak, Don-Kyu Kim, Moon-Hee Sung, Suryang Kwak

{"title":"Evaluation of the Safety and Impact of Heat-Treated Lactiplantibacillus plantarum KM2 Fermentation on Gut Microbiome Architecture.","authors":"Seok Won, Yejin Jeong, Ji-Eun Kim, Jong-Hoon Kim, Hyun Soo Song, Hyun-Hwa Bae, Mi-Sun Kwak, Don-Kyu Kim, Moon-Hee Sung, Suryang Kwak","doi":"10.4014/jmb.2411.11069","DOIUrl":"10.4014/jmb.2411.11069","url":null,"abstract":"Postbiotics, bioactive compounds from the fermentation process by probiotics, are gaining attention for their potential health benefits as safer alternatives to live probiotic microbes. Lactiplantibacillus plantarum is a well-studied probiotic species known for promoting gut health and immune modulation. However, the safety and effects of its postbiotic formulations on the gut microbiome structure remain less explored. This study presents a randomized, double-blind, placebo-controlled human study of KLP-KM2, a postbiotic consisting of heat-treated L. plantarum KM2 fermentation complex, in elderly participants. Over 12 weeks, KLP-KM2 consumption did not result in noticeable adverse reaction cases compared to the placebo. Nevertheless, the gut microbial diversity and taxonomic architecture of the KLP-KM2 recipients were differentiated from those of the placebo recipients after 12 weeks. A notable outcome was the increase in the number of subjects carrying Veillonella spp., which contributed to the distinct gut microbiome profiles observed between the two groups. Interestingly, KLP-KM2 facilitated the de novo colonization of Veillonella spp. in subjects who had not harbored these bacteria at the baseline. These results suggest the potential of KLP-KM2 as a safe and effective postbiotic intervention to enhance energy metabolism and mobility in older adults.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411069"},"PeriodicalIF":2.5,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety, Antagonistic Activity, and Probiotic Properties of Lactic Acid Bacteria Isolated from Jeotgal, Korean Fermented Seafoods.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-12-13 DOI: 10.4014/jmb.2411.11055

Jihyeon Baek, Bong Sun Kim, Yeonju Kim, Jaewoo Bai

{"title":"Safety, Antagonistic Activity, and Probiotic Properties of Lactic Acid Bacteria Isolated from Jeotgal, Korean Fermented Seafoods.","authors":"Jihyeon Baek, Bong Sun Kim, Yeonju Kim, Jaewoo Bai","doi":"10.4014/jmb.2411.11055","DOIUrl":"https://doi.org/10.4014/jmb.2411.11055","url":null,"abstract":"Probiotics are in high demand in the health functional food market as they effectively inhibit pathogens and improve host health. Therefore, in order to develop novel probiotic strains, new strains were isolated from various type of jeotgal, traditional Korean fermented seafood products, and their safety and probiotic properties have been evaluated. Based on 16S rRNA gene sequence analysis, six strains (JRD1, Pediococcus pentosaceus; JRD2, Lactiplantibacillus plantarum; JRD6, Pediococcus acidilactici; CLJ21, Lactiplantibacillus plantarum; CLJ24, Pediococcus pentosaceus; CLJ28, Leuconostoc mesenteroides subsp. dextranicum) were selected and subjected to further analysis. As a result, all six strains did not show hemolytic activity, antibiotics resistance, and cell cytotoxicity, confirming that they are safe for human use. Among them, JRD1, JRD6, and CLJ24 exhibited high survival rates under simulated gastrointestinal conditions. Additionally, these three strains demonstrated strong adhesion abilities on HT-29 cells, with values of 6.02, 5.77, and 5.86 log CFU/mL, respectively. Furthermore, JRD1, JRD6, and CLJ24 showed relatively high antagonistic activity against both Salmonella Typhimurium and Staphylococcus aureus through competition, exclusion, and displacement of their adhesion. Interestingly, cell-free supernatants (CFS) from three strains effectively inhibited the growth of both S. Typhimurium and S. aureus. Furthermore, CFS of CLJ24, JRD1, and JRD6 demonstrated anti-inflammatory effects in intestinal epithelial cells. The results suggest that CLJ24, JRD1, and JRD6 have potential to be development as functional probiotic strains with both antibacterial and anti-inflammatory activities.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411055"},"PeriodicalIF":2.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of a Small-Molecule Inhibitor Targeting the Biofilm Regulator BrpT in Vibrio vulnificus. 发现针对弧菌生物膜调节器 BrpT 的小分子抑制剂

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-20 DOI: 10.4014/jmb.2406.06052

Wonwoo Choi, Hojun Lee, Qiyao Wang, Ye-Ji Bang, Sang Ho Choi

{"title":"Discovery of a Small-Molecule Inhibitor Targeting the Biofilm Regulator BrpT in Vibrio vulnificus.","authors":"Wonwoo Choi, Hojun Lee, Qiyao Wang, Ye-Ji Bang, Sang Ho Choi","doi":"10.4014/jmb.2406.06052","DOIUrl":"10.4014/jmb.2406.06052","url":null,"abstract":"Vibrio vulnificus, an opportunistic human pathogen, employs biofilm formation as a key survival and virulence mechanism. BrpT, a transcriptional regulator, is essential for V. vulnificus biofilm development by regulating the expression of biofilm-related genes. In this study, we aimed to identify a small molecule inhibitor of BrpT to combat V. vulnificus biofilm formation. High-throughput screening of 7,251 compounds using an Escherichia coli reporter strain carrying the arabinose-inducible brpT gene and a BrpT-activated promoter fused to the luxCDABE operon identified a hit compound, BTI (BrpT Inhibitor). BTI potently inhibited BrpT activity in V. vulnificus (EC50 of 6.48 μM) without affecting bacterial growth or host cell viability. Treatment with BTI significantly reduced the expression of the BrpT regulon and impaired biofilm formation and colony rugosity in V. vulnificus, thus increasing its susceptibility to antibiotics. In vitro biochemical analyses revealed that BTI directly binds to BrpT and inhibits its transcriptional regulatory activity. The identification of BTI as a specific inhibitor of BrpT that effectively diminishes V. vulnificus biofilm formation provides a promising foundation for the development of novel anti-biofilm strategies, with the potential to address the growing challenge of antibiotic resistance and improve the treatment of biofilm-associated infections.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2201-2210"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Heavy Metal-Tolerant and Anti-Phytopathogenic Plant Growth-Promoting Bacteria from Soils. 从土壤中分离出耐受重金属和抗植物病原菌的植物生长促进细菌。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-10-28 DOI: 10.4014/jmb.2407.07013

Soo Yeon Lee, Kyung-Suk Cho

{"title":"Isolation of Heavy Metal-Tolerant and Anti-Phytopathogenic Plant Growth-Promoting Bacteria from Soils.","authors":"Soo Yeon Lee, Kyung-Suk Cho","doi":"10.4014/jmb.2407.07013","DOIUrl":"10.4014/jmb.2407.07013","url":null,"abstract":"In this study, multifunctional soil bacteria, which can promote plant development, resist heavy metals, exhibit anti-phytopathogenic action against plant diseaes, and produce extracellular enzymes, were isolated to improve the effectiveness of phytoremediation techniques. In order to isolate multifunctional soil bacteria, a variety of soil samples with diverse characteristics were used as sources for isolation. To look into the diversity and structural traits of the bacterial communities, we conducted amplicon sequencing of the 16S rRNA gene on five types of soils and predicted functional genes using Tax4Fun2. The isolated bacteria were evaluated for their multifunctional capabilities, including heavy metal tolerance, plant growth promotion, anti-phytopathogenic activity, and extracellular enzyme activity. The genes related to plant growth promotion and anti-phytopathogenic activity were most abundant in forest and paddy soils. Burkholderia sp. FZ3 and FZ5 demonstrated excellent heavy metal resistance (≤ 1 mM Cd and ≤ 10 mM Zn), Pantoea sp. FC24 exhibited the highest protease activity (24.90 μmol tyrosine·g-DCW-1·h-1), and Enterobacter sp. PC20 showed superior plant growth promotion, especially in siderophore production. The multifunctional bacteria isolated using traditional methods included three strains (FC24, FZ3, and FZ5) from the forest and one strain (PC20) from paddy field soil. These results indicate that, for the isolation of beneficial soil microorganisms, utilizing target gene information obtained from isolation sources and subsequently exploring target microorganisms is a valuable strategy.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2252-2265"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Jasminum subtriplinerve Extract against 7,12-Dimethylbenz[a]anthracene-Induced Cancer in Mice. Jasminum subtriplinerve 提取物对 7,12-二甲基苯并[a]蒽诱发的小鼠癌症的疗效。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-20 DOI: 10.4014/jmb.2407.07045

Phan Hong Minh, Pham Thi Van Anh, Bui Thanh Tung, Ho My Dung, Tran Thi Thu Trang, Pham Thi Hong Nhung, Nguyen Thi Hang, Nguyen Thi Minh Nguyet, Nguyen Viet Phong, Le Ba Vinh, Mai Phuong Thanh

{"title":"Efficacy of Jasminum subtriplinerve Extract against 7,12-Dimethylbenz[a]anthracene-Induced Cancer in Mice.","authors":"Phan Hong Minh, Pham Thi Van Anh, Bui Thanh Tung, Ho My Dung, Tran Thi Thu Trang, Pham Thi Hong Nhung, Nguyen Thi Hang, Nguyen Thi Minh Nguyet, Nguyen Viet Phong, Le Ba Vinh, Mai Phuong Thanh","doi":"10.4014/jmb.2407.07045","DOIUrl":"10.4014/jmb.2407.07045","url":null,"abstract":"Jasminum subtriplinerve Blume tea is a traditional Vietnamese medicine used to treat impetigo, menstruation issues, and painful menstrual hematometra. Previous studies have shown that extracts and isolated compounds from J. subtriplinerve possess diverse pharmacological properties, such as antioxidant, antibacterial, and antidiabetic effects. However, their potential anticancer effects and underlying mechanisms of action have not been clear. Here, we examined the effects of J. subtriplinerve extracts against three human cancer cell lines. We also conducted in vivo analyses using a mouse model of 7,12-dimethylbenz[a]anthracene-induced breast cancer, including an investigation of changes in histological sections. The effect of the J. subtriplinerve ethyl acetate fraction on cytokine levels (IL-2, PGE2, TNF-α) in serum was determined using ELISA kits. Results showed that the ethyl acetate (EtOAc) fraction had the highest anti-proliferative activity (IC50 = 13.7 mg/ml) against the breast cancer (MCF-7) cell line, while the butanol (BuOH) and water fractions did not show any anticancer effects. Additionally, the EtOAc fraction at a dose of 14.4 mg/kg was able to elevate IL-2 levels and suppress the expression of PGE2 in the serum of mice. A remarkable decrease in the percentage of death and tumor incidence in mice was achieved following treatment with the EtOAc fraction at a dose of 14.4mg/kg. No abnormal parameters in blood were observed in the J. subtriplinerve treatment groups. These results suggest that J. subtriplinerve, when used as tea or a functional food, is nontoxic and has clear chemopreventive effects against breast cancer.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2173-2183"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Activity of Cold Atmospheric Plasma on Bacterial Strains Derived from Patients with Diabetic Foot Ulcers. 冷大气等离子体对糖尿病足溃疡患者细菌菌株的抗菌活性

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-30 DOI: 10.4014/jmb.2407.07035

Roopak Murali, Pooja Singh, Divya Ragunathan, Ramya Damarla, Dharshini Kichenaradjou, Kirtanna Malichetty Surriyanarayanan, Satish Kumar Jayaram, Harish C Chandramoorthy, Ashish Kumar, Mary Elizabeth Gnanambal Krishnan, Rajesh Kumar Gandhirajan

{"title":"Antimicrobial Activity of Cold Atmospheric Plasma on Bacterial Strains Derived from Patients with Diabetic Foot Ulcers.","authors":"Roopak Murali, Pooja Singh, Divya Ragunathan, Ramya Damarla, Dharshini Kichenaradjou, Kirtanna Malichetty Surriyanarayanan, Satish Kumar Jayaram, Harish C Chandramoorthy, Ashish Kumar, Mary Elizabeth Gnanambal Krishnan, Rajesh Kumar Gandhirajan","doi":"10.4014/jmb.2407.07035","DOIUrl":"10.4014/jmb.2407.07035","url":null,"abstract":"Bacterial infections or their biofilms in diabetic foot ulcer (DFU) are a key cause of drug-resistant wounds and amputations. Cold atmospheric plasma (CAP) is well documented for its antibacterial effect and promoting wound healing. In the current study, we built an argon-based, custom CAP device and investigated its potential in eliminating laboratory and clinical bacterial strains derived from DFU. The CAP device performed as expected with generation of hydroxyl, reactive nitrogen species, and argon species as determined by optical emission spectroscopy. A dose-dependent increase in oxidation reduction potential (ORP) and nitrites in the liquid phase was observed. The CAP treatment eliminated both gram-positive (Staphylococcus aureus, Entrococcus faecalis) and negative bacteria (Pseudomonas aeruginosa, Proteus mirabilis) laboratory strains. Clinical samples collected from DFU patients exhibited a significant decrease in both types of bacteria, with gram-positive strains showing higher susceptibility to the CAP treatment in an ex vivo setting. Moreover, exposure to CAP of polymicrobial biofilms from DFU led to a notable disruption in biofilm and an increase in free bacterial DNA. The duration of CAP exposure used in the current study did not induce DNA damage in peripheral blood lymphocytes. These results suggest that CAP could serve as an excellent tool in treating patients with DFUs.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2353-2361"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deleterious Effects of Histidine Tagging to the SH3b Cell Wall-Binding Domain on Recombinant Endolysin Activity. 组氨酸标记到 SH3b 细胞壁结合域对重组内溶酶活性的有害影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-10-11 DOI: 10.4014/jmb.2408.08003

Jin-Mi Park, Jun-Hyun Kim, Kang-Seuk Choi, Hyuk-Joon Kwon

{"title":"Deleterious Effects of Histidine Tagging to the SH3b Cell Wall-Binding Domain on Recombinant Endolysin Activity.","authors":"Jin-Mi Park, Jun-Hyun Kim, Kang-Seuk Choi, Hyuk-Joon Kwon","doi":"10.4014/jmb.2408.08003","DOIUrl":"10.4014/jmb.2408.08003","url":null,"abstract":"Natural and artificial endolysins exhibit bactericidal effects by destroying peptidoglycans in the cell wall of gram-positive bacteria and are usually composed of an N-terminal catalytic domain (CTD) and a C-terminal cell wall-binding domain (CBD). The structures and receptors of CBDs are variable, but bacterial Src homology 3 (SH3b) CBDs are prevalent among the natural endolysins of Staphylococcus aureus. Moreover, although recombinant endolysins with a C-terminal 6x histidine tag (His-tag) are often produced and convenient to purify, the deleterious effects of His-tags on antibacterial activity have not been evaluated thoroughly. Recently, we reported that the antibacterial activity of a commercial lysostaphin without a His-tag differed from that of cell-free lysostaphin with a C-terminal His-tag, and lysostaphin also contains a C-terminal SH3b CBD. In this study, we directly compared the effects of His-tags on the antibacterial activities of lysostaphin and several chimeric lysins possessing different SH3b CBDs. We confirmed that antibacterial activity decreased 16.0-32.0-fold after a His-tag was added to the SH3b CBD.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2331-2337"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Recombinant Antibodies and Its Application in Immunomagnetic Separation-Based Rapid Detection of Vibrio cholerae in Aquatic Environments. 重组抗体的开发及其在基于免疫磁分离的水生环境霍乱弧菌快速检测中的应用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-10-01 DOI: 10.4014/jmb.2405.05003

Haiyang Zhang, Quan Wang, Haoxiang Zhuang, Qiu Lin, Wenchao Wang, Fangyu Ye, Saqib Nawaz, Jiangang Hu, Cuiqin Huang, Huifang Yin, Weidong Sun, Xiangan Han, Wei Jiang

{"title":"Development of Recombinant Antibodies and Its Application in Immunomagnetic Separation-Based Rapid Detection of Vibrio cholerae in Aquatic Environments.","authors":"Haiyang Zhang, Quan Wang, Haoxiang Zhuang, Qiu Lin, Wenchao Wang, Fangyu Ye, Saqib Nawaz, Jiangang Hu, Cuiqin Huang, Huifang Yin, Weidong Sun, Xiangan Han, Wei Jiang","doi":"10.4014/jmb.2405.05003","DOIUrl":"10.4014/jmb.2405.05003","url":null,"abstract":"Cholera caused by Vibrio cholerae remains a major public health concern in many countries. The greatest obstacle to detection of V. cholerae contamination in drinking water or aquatic environments mainly relates to sample preparation steps, especially the enrichment step. In this study, immunomagnetic separation methods were developed based on sequence-defined recombinant antibodies (rAbs) against V. cholerae, then used for the specific and efficient enrichment of V. cholerae in water samples. Using the variable region genes of the anti-V. cholerae monoclonal antibodies (mAbs) 5F2, the full-length IgG rAbs (R5F2) were produced using mammalian human embryonic kidney 293T cells. Two antibodies, 5F2 and R5F2, were used to prepare immunomagnetic beads (IMBs), and their capture efficiencies (CEs) were evaluated. The results showed that 0.4 mg of 5F2-IMBs and R5F2-IMBs exhibited good CEs (96.0% and 75.9%, respectively) against V. cholerae within 40 min. The IMBs could still effectively capture V. cholerae in large-volume reaction systems (5 ml to 25 ml). The CEs of 5F2-IMBs and R5F2-IMBs ranged from 90.2% to 70.7% and 65.1% to 44.2%, respectively. Furthermore, 5F2-IMBs and R5F2-IMBs did not show significant cross-reactivity with other bacteria and exhibited high specificity. When R5F2-IMS was used in combination with quantitative real-time PCR, the detection limit was approximately 5 colony-forming units/25 ml after enrichment for 4 h. Our results suggest that the rAbs produced herein could provide useful alternatives to traditional hybridoma-based antibodies for accurate detection of V. cholerae in food safety and environmental monitoring.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2266-2278"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Control of Cucumber Powdery Mildew Using Resistant Cultivars and Organic Agricultural Materials.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 DOI: 10.4014/jmb.2409.09030

Min-Jeong Kim, Yong-Ki Kim, So-Hyang Park, Jong-Ho Park, Sung-Jun Hong, Chang-Ki Shim

{"title":"Control of Cucumber Powdery Mildew Using Resistant Cultivars and Organic Agricultural Materials.","authors":"Min-Jeong Kim, Yong-Ki Kim, So-Hyang Park, Jong-Ho Park, Sung-Jun Hong, Chang-Ki Shim","doi":"10.4014/jmb.2409.09030","DOIUrl":"10.4014/jmb.2409.09030","url":null,"abstract":"This study evaluated the efficacy of various inorganic materials, biological control agents, organic agricultural materials (OAMs), and essential oils for controlling cucumber powdery mildew (CPM) under greenhouse conditions. Treatments included silicate, bicarbonate, copper sulfate, sulfur-based compounds, Bacillus subtilis KB-401, Bacillus amyloliquefaciens M27, Bordeaux mixtures, chitosan combined with oxidated copper salt, chitooligosaccharides, and essential oils such as castor and rapeseed oil with lecithin. Results demonstrated that Bordeaux mixtures, particularly Bordeaux Mixture I, and sulfur-based compounds provided the most reliable CPM control among inorganic materials. Bacillus strains KB-401 and M27 achieved sustained disease suppression, suggesting their value in integrated pest management (IPM). Chitosan combined with oxidated copper salt showed high efficacy, making it a promising candidate for long-term control. Among OAMs, loess-sulfur mixtures proved especially effective in preventive applications, achieving comparable results to resistant cultivar 'Saronsamcheok'. Essential oils, including castor and rapeseed oil, also exhibited significant CPM suppression potential, although repeated applications may be necessary for sustained control. These findings highlight the potential of these environmentally friendly treatments as viable components of an IPM strategy for managing CPM in cucumber crops. Further studies are recommended to optimize combinations and application timing for enhanced efficacy.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2409030"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0