Hyo-Min Kim, Dan-Hee Yoo, Jung-Wook Kang, In-Chul Lee, Jong-Sup Bae
{"title":"Anti-Inflammatory Effect of Extract from <i>Fragaria ananass</i>a Duch. Calyx via MAPK and NF-κB Signaling Pathway.","authors":"Hyo-Min Kim, Dan-Hee Yoo, Jung-Wook Kang, In-Chul Lee, Jong-Sup Bae","doi":"10.4014/jmb.2409.09044","DOIUrl":"https://doi.org/10.4014/jmb.2409.09044","url":null,"abstract":"<p><p>Currently, Fragaria ananassa Duch. are discarded as by-products except for the fruit part, so we developed a natural material using the top (= calyx), one of the by-products, and prepared an extract using 70% ethanol to investigate its effects on anti-inflammatory mechanisms. The polyphenol content of 70% ethanol extracts from Fragaria ananassa Duch. calyx was measured to be 265.86 ± 0.85 mg TAE/100 g, respectively. The antioxidant activity was confirmed through the electron donating ability and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging ability measurements. When extracts from Fragaria ananassa Duch. calyx was treated to LPSinduced RAW 264.7 cells, it was confirmed that the production of inflammation-related factors, NO, PGE2, iNOS, COX-2, TNF-a, and IL-6, was inhibited. In addition, it was confirmed that extracts from Fragaria ananassa Duch. calyx affected the MAPK signaling pathway by reducing the protein expression of p-ERK, p-JNK, and p-p38, which are the upper signaling pathways. In addition, it was confirmed to reduce the protein expression of p-p65 and p-IkB, which are NF-κB signaling pathways. Therefore, this study suggests that extracts from Fragaria ananassa Duch. calyx affect the regulation of the production of major inflammation-related factors by inhibiting the MAPK and NF-κB signaling pathway. These results confirmed that extracts from Fragaria ananassa Duch. calyx have the potential to be developed as a new natural material with anti-inflammatory activity.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"1-13"},"PeriodicalIF":2.5,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ELF3 Overexpression Contributes to the Malignant Transformation of HPV16 E6/ E7-Immortalized Keratinocytes by Promoting CCNE2 Expression.","authors":"Yingping Zhu, Wenjuan Yang, Yulong Zhuang, Feifei Wang, Yanlu Ge, Jun Jiang, Danping Feng","doi":"10.4014/jmb.2408.08041","DOIUrl":"https://doi.org/10.4014/jmb.2408.08041","url":null,"abstract":"<p><p>Current cancer burden caused by persistent infection with human papillomaviruse genotype 16 (HPV16) cannot be ignored. The related mechanisms of oncoproteins E6 and E7 from HPV16 on keratinocyte malignant transformation need to be further elucidated. GSE3292 dataset analysis revealed the upregulation of ETS transcription factor 3 (ELF3) and cyclin E2 (CCNE2). To verify whether there is an interaction between ELF3 and CCNE2, E74 like ELF3 and CCNE2 expression profiles as well as their putative binding sites were analyzed using bioinformatics. Retroviruses encoding HPV16 E6 and E7 genes were used to induce immortalization of human foreskin keratinocytes (HFKs) in vitro. Dual luciferase reporters assay was used to verify the binding of ELF3 and CCNE2. The effect of ELF3 on the immortalized cells was investigated using CCK-8 assay, cell cycle analysis and western blot. ELF3 and CCNE2 presented overexpression patterns in head and neck squamous cell carcinoma. HPV16 E6/E7-expressing HFKs showed enhanced viability, accelerated cell cycle as well as upregulated ELF3 and CCNE2. ELF3 overexpression enhanced the activity of CCNE2 promoter. ELF3 silencing reduced viability, induced cell cycle arrest and suppressed expressions of CCNE2, E6 and E7 in HPV16 E6/E7-expressing HFKs. Downregulation of ELF3 played an inhibiting role in the malignant transformation of HPV16 E6/E7-immortalized HFKs by decreasing CCNE2 expression.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"1-8"},"PeriodicalIF":2.5,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ji-Won Choi, Sang Yoon Choi, Guijae Yoo, Ho-Young Park, In-Wook Choi, Jinyoung Hur
{"title":"<i>Melissa officinalis</i> Regulates Lipopolysaccharide-Induced BV2 Microglial Activation via MAPK and Nrf2 Signaling.","authors":"Ji-Won Choi, Sang Yoon Choi, Guijae Yoo, Ho-Young Park, In-Wook Choi, Jinyoung Hur","doi":"10.4014/jmb.2409.09020","DOIUrl":"https://doi.org/10.4014/jmb.2409.09020","url":null,"abstract":"<p><p>Neuroinflammation and microglial activation play critical roles in neurodegenerative diseases such as Alzheimer's and Parkinson's disease. Modulating microglial activation may help prevent the progression of these disorders. This study aimed to investigate the effects and mechanisms of <i>Melissa officinalis</i> ethanol extract on lipopolysaccharide (LPS)-induced microglial activation in BV2 cells. Cell viability and nitric oxide (NO) production were assessed using MTT assay and Griess reagent, while inflammatory cytokine levels were measured by qPCR. Key inflammatory pathways, including MAPK, TLR4, and antioxidant biomarkers, were analyzed through western blot and immunofluorescence. Rosmarinic acid content in <i>M.</i> <i>officinalis</i> was determined using high-performance liquid chromatography (HPLC). The results demonstrated that M. officinalis ethanol extract significantly inhibited LPS-induced NO production and reduced inflammatory cytokine expression. Additionally, it downregulated inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TLR4, NF-κB, and MAPK signaling pathways (p38, JNK, ERK), while increasing the expression of antioxidant markers, including Nrf2, HO-1, catalase, and SOD2. In conclusion, <i>M. officinalis</i> ethanol extract exerts neuroprotective effects by modulating inflammation and enhancing antioxidant defenses, suggesting its potential in the prevention and treatment of inflammation-related neurodegenerative diseases.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soo-Jeong Lee, Jihye Yang, Gi Beom Keum, Jinok Kwak, Hyunok Doo, Sungwoo Choi, Dong-Geun Park, Chul-Hong Kim, Hyeun Bum Kim, Ju-Hoon Lee
{"title":"Therapeutic Potential of <i>Lactiplantibacillus plantarum</i> FB091 in Alleviating Alcohol-Induced Liver Disease through Gut-Liver Axis.","authors":"Soo-Jeong Lee, Jihye Yang, Gi Beom Keum, Jinok Kwak, Hyunok Doo, Sungwoo Choi, Dong-Geun Park, Chul-Hong Kim, Hyeun Bum Kim, Ju-Hoon Lee","doi":"10.4014/jmb.2407.07051","DOIUrl":"10.4014/jmb.2407.07051","url":null,"abstract":"<p><p>Alcoholic liver disease (ALD) poses a significant global health burden, often requiring liver transplantation and resulting in fatalities. Current treatments, like corticosteroids, effectively reduce inflammation but carry significant immunosuppressive risks. This study evaluates <i>Lactiplantibacillus plantarum</i> FB091, a newly isolated probiotic strain, as a safer alternative for ALD treatment. Using an in vivo mouse model, we assessed the effects of <i>L. plantarum</i> FB091 on alcohol-induced liver damage and gut microbiota composition. Alcohol and probiotics administration did not significantly impact water/feed intake or body weight. Histopathological analysis showed that <i>L. plantarum</i> FB091 reduced hepatocellular ballooning and inflammatory cell infiltration in liver tissues and mitigated structural damage in colon tissues, demonstrating protective effects against alcohol-induced damage. Biomarker analysis indicated that <i>L. plantarum</i> FB091 decreased aspartate aminotransferase levels, suggesting reduced liver damage, and increased alcohol dehydrogenase activity, indicating enhanced alcohol metabolism. Additionally, cytokine assays revealed a reduction in pro-inflammatory TNF-α and an increase in anti-inflammatory IL-10 levels in colon tissues of the <i>L. plantarum</i> FB091 group, suggesting an anti-inflammatory effect. Gut microbiota analysis showed changes in the <i>L. plantarum</i> FB091 group, including a reduction in Cyanobacteria and an increase in beneficial bacteria such as <i>Akkermansia</i> and <i>Lactobacillus</i>. These changes correlated with the recovery and protection of liver and colon health. Overall, <i>L. plantarum</i> FB091 shows potential as a therapeutic probiotic for managing ALD through its protective effects on liver and colon tissues, enhancement of alcohol metabolism, and beneficial modulation of gut microbiota. Further clinical studies are warranted to confirm these findings in humans.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 10","pages":"2100-2111"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation and Molecular Characterization of Colistin-Resistant Isolates of <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i> from the Infected Wounds of Hospitalized Patients.","authors":"Munaza Ijaz, Madiha Khan, Haya Yasin","doi":"10.4014/jmb.2407.07005","DOIUrl":"10.4014/jmb.2407.07005","url":null,"abstract":"<p><p>This study was planned to determine the colistin-resistant (CR) gene distribution among two species of gram-negative bacteria, <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i>. In total, 50 isolates of <i>K. pneumoniae</i> (14 isolates, 28%) and <i>P. aeruginosa</i> (36 isolates, 72%) were isolated between August 2023 and October 2023 from clinical wound samples at Jinnah Hospital and Lahore General Hospital Lahore, Pakistan. To determine the resistance genes linked to CR and assess antimicrobial susceptibility, all isolates were kept at -80°C in 15% glycerol broth. Using the right primer sets, a polymerase chain reaction (PCR) was utilized to identify the CR-associated <i>mcr-1</i> gene of the gram-negative isolates. Out of 50, 40 isolates (80%) showed resistance against colistin with MICs of 8 and 128 μg/ml. The majority (97%) of <i>P. aeruginosa</i> CR strains were considered multidrug resistant (MDR). All <i>K. pneumoniae</i> isolates were resistant to cefepime, cotrimoxazole, ceftriaxone, and imipenem. The clinical CR isolates of <i>P. aeruginosa</i> were highly resistant to ceftriaxone, imipenem, cefepime, cotrimoxazole, ciprofloxacin, amikacin, and piperacillin/tazobactum. The antibiotic resistance pattern was terrifyingly high among both bacterial species. According to the PCR results, CR was prevalent among the gram-negative samples, and the <i>mcr-1</i> gene was positive in 6/40 (15%) of the CR isolates, including four <i>P. aeruginosa</i> and two <i>K. pneumoniae</i> strains. The high CR (80%) reported in this research is cause for concern and underscores an urgent need to use colistin in a limited and logical manner, similar to other antibiotics.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 10","pages":"1981-1987"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yi Xu, Xiahui Wu, Yan Li, Xuejie Liu, Lijian Fang, Ziyu Jiang
{"title":"Probiotics and the Role of Dietary Substrates in Maintaining the Gut Health: Use of Live Microbes and Their Products for Anticancer Effects against Colorectal Cancer.","authors":"Yi Xu, Xiahui Wu, Yan Li, Xuejie Liu, Lijian Fang, Ziyu Jiang","doi":"10.4014/jmb.2403.03056","DOIUrl":"10.4014/jmb.2403.03056","url":null,"abstract":"<p><p>The gut microbiome is an important and the largest endocrine organ linked to the microbes of the GI tract. The bacterial, viral and fungal communities are key regulators of the health and disease status in a host at hormonal, neurological, immunological, and metabolic levels. The useful microbes can compete with microbes exhibiting pathogenic behavior by maintaining resistance against their colonization, thereby maintaining eubiosis. As diagnostic tools, metagenomic, proteomic and genomic approaches can determine various microbial markers in clinic for early diagnosis of colorectal cancer (CRC). Probiotics are live non-pathogenic microorganisms such as lactic acid bacteria, <i>Bifidobacteria</i>, <i>Firmicutes</i> and <i>Saccharomyces</i> that can help maintain eubiosis when administered in appropriate amounts. In addition, the type of dietary intake contributes substantially to the composition of gut microbiome. The use of probiotics has been found to exert antitumor effects at preclinical levels and promote the antitumor effects of immunotherapeutic drugs at clinical levels. Also, modifying the composition of gut microbiota by Fecal Microbiota Transplantation (FMT), and using live lactic acid producing bacteria such as <i>Lactobacillus</i>, <i>Bifidobacteria</i> and their metabolites (termed postbiotics) can contribute to immunomodulation of the tumor microenvironment. This can lead to tumor-preventive effects at early stages and antitumor effects after diagnosis of CRC. To conclude, probiotics are presumably found to be safe to use in humans and are to be studied further to promote their appliance at clinical levels for management of CRC.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 9","pages":"1933-1946"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characteristics of Recombinant <i>Chlamydomonas reinhardtii</i> Expressing Putative Germin-Like Protein from <i>Neopyropia yezoensis</i>.","authors":"Jiae Kim, Jong-Il Choi","doi":"10.4014/jmb.2407.07059","DOIUrl":"10.4014/jmb.2407.07059","url":null,"abstract":"<p><p>Since microalgae face various environmental stresses for the high production of biofuels, multiple studies have been performed to determine if microalgae are resistant to these various stresses. In this study, the viability of cells under various abiotic stresses was investigated by introducing a putative germin-like protein (GLP) from <i>Neopyropia yezoensis</i>, which was known to be related in the resistance to abiotic stresses. The expression of GLP in <i>Chlamydomonas reinhardtii</i> allowed cells to grow better in various abiotic stress environments. In nitrogen starvation conditions, recombinant cells accumulated the lipid droplet 1.46-fold more than wild-type cells and responded more rapidly to form palmelloid forms. Under high-temperature, hydrogen peroxide conditions and saline stress, the survival rate was increased 3.5 times, 2.19 times, and 3.19 times in recombinant <i>C. reinhardtii</i> with GLP, respectively. The expression level of genes related to pathways in response to various stresses increased 2-fold more under those conditions. This result will be useful for the development of microalgae that can grow better and produce more biofuels under different stress conditions.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 10","pages":"2132-2141"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chi Young Hwang, Eui-Sang Cho, Eun Hee Bae, Dong-Hyun Jung, Myung-Ji Seo
{"title":"Carotenoid-Producing <i>Paracoccus aurantius</i> sp. nov., Isolated from the West Coast of Dokdo Island, Republic of Korea.","authors":"Chi Young Hwang, Eui-Sang Cho, Eun Hee Bae, Dong-Hyun Jung, Myung-Ji Seo","doi":"10.4014/jmb.2404.04053","DOIUrl":"10.4014/jmb.2404.04053","url":null,"abstract":"<p><p>In this study, a novel species within the genus <i>Paracoccus</i> was isolated from the coastal soil of Dokdo (Seodo) Island and investigated. We elucidated the novel species, designated MBLB3053<sup>T</sup>, through genomic analysis of novel functional microbial resources. Cells were gram-negative, non-motile, and coccoid, and the colony was light orange in color. Phylogenetic analysis based on the 16S rRNA gene showed that strain MBLB3053<sup>T</sup> was related to the genus <i>Paracoccus</i>, with 98.5% similarity to <i>Paracoccus aestuariivivens</i>. Comparative genome analysis also revealed the strain to be a novel species of the genus <i>Paracoccus</i> by average nucleotide identity and in silico DNA-DNA hybridization values. Through secondary metabolite analysis, terpene biosynthetic gene clusters associated with carotenoid biosynthesis were found in strain MBLB3053<sup>T</sup>. Using high-performance liquid chromatography, strain MBLB3053<sup>T</sup> was confirmed to produce carotenoids, including all-<i>trans</i>-astaxanthin, by comparison to the standard compound. Notably, the isolate was also confirmed to produce carotenoids that other closely related species did not produce. Based on this comprehensive polyphasic taxonomy, strain MBLB3053<sup>T</sup> represents a novel species within the genus <i>Paracoccus</i>, for which the name <i>Paracoccus aurantius</i> sp. nov is proposed. The type strain was MBL3053<sup>T</sup> (=KCTC 8269<sup>T</sup> =JCM 36634<sup>T</sup>). These findings support the research and resource value of this novel species, which was isolated from the Dokdo environmental microbiome.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 10","pages":"2012-2022"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shin-Hye Kim, Hye-Lim Shin, Tae Hyun Son, Dongsoo Kim, Hwan-Gyu Kim, Jae-Han Cho, Sik-Won Choi
{"title":"The Biphasic Activity of Auricularia Auricula-Judae Extract on Bone Homeostasis Through Inhibition of Osteoclastogenesis and Modulation of Osteogenic Activity.","authors":"Shin-Hye Kim, Hye-Lim Shin, Tae Hyun Son, Dongsoo Kim, Hwan-Gyu Kim, Jae-Han Cho, Sik-Won Choi","doi":"10.4014/jmb.2408.08055","DOIUrl":"https://doi.org/10.4014/jmb.2408.08055","url":null,"abstract":"<p><p>Osteoporosis arises from the disturbance of bone homeostasis, a process regulated by osteoblasts and osteoclasts. The treatment and prevention of bone metabolic disorders resulting from an imbalance in bone homeostasis require the use of agents that effectively promote both bone formation and anti-resorptive effects. Therefore, an investigation was carried out to determine the potential of the edible mushroom <i>Auricularia auricula-judae</i> in modulating bone remodeling by inhibiting RANKL-induced osteoclastogenesis and enhancing BMP-2-stimulated osteoblast differentiation. Moreover, this study assessed the mode of action of the <i>Auricularia auricula-judae</i> extracts. The staining of tartrate-resistant acid phosphatase (TRAP), a marker for osteoclast activity, demonstrated that Auricularia auricula-judae water extract (AAJWE) inhibited the formation of multinucleated osteoclasts while exhibiting no cytotoxic effects. The study demonstrated that AAJWE reduced RANKL-induced osteoclast differentiation by inhibiting c-Fos/NFATc1 through the inhibition of ERK and JNK phosphorylation during the RANKL-induced osteoclast differentiation. Moreover, AAJWE exhibited a dose-dependent induction of ALP expression in the presence of BMP-2 during osteoblast differentiation. The AAJWE strengthened BMP-2-induced osteogenesis through the activation of Runx2 and Smad phosphorylation. Therefore, AAJWE emerges as a promising candidate for both prevention and therapy owing to its biphasic effect, which aids in the preservation of bone homeostasis.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raul Joao Lourenco Mascarenha, Du-Min Jo, Yoon-Ah Sim, Do-Hyung Kim, Young-Mog Kim
{"title":"Synergistic Antibacterial Effect of <i>Eisenia bicyclis</i> Extracts in Combination with Antibiotics against Fish Pathogenic Bacteria.","authors":"Raul Joao Lourenco Mascarenha, Du-Min Jo, Yoon-Ah Sim, Do-Hyung Kim, Young-Mog Kim","doi":"10.4014/jmb.2406.06027","DOIUrl":"10.4014/jmb.2406.06027","url":null,"abstract":"<p><p>The aquaculture industry faces significant challenges due to bacterial infections caused by <i>Edwardsiella tarda</i>, <i>Photobacterium damselae</i>, and <i>Vibrio harveyi</i>. The extensive use of traditional antibiotics, has resulted in widespread antibiotic resistance. This study aimed to investigate the antibacterial potential of the brown seaweed <i>Eisenia bicyclis</i>, particularly its synergistic effects with antibiotics against these fish pathogenic bacteria. <i>E. bicyclis</i> were processed to obtain methanolic extracts and fractionated using different polar solvents. The antibacterial activities of these extracts and fractions were assessed through disc diffusion and minimum inhibitory concentration (MIC) assays. The study further evaluated the antibiotic susceptibility of the bacterial strains and the synergistic effects of the extracts combined with erythromycin and oxyteteracycline using the fractional inhibitory concentration index. Results showed that the ethyl acetate (EtOAc) fraction of <i>E. bicyclis</i> methanolic extract exhibited the highest antibacterial activity. The combination of the EtOAc fraction with erythromycin significantly enhanced its antibacterial efficacy against the tested strains. This synergistic effect was indicated by a notable reduction in MIC values, demonstrating the potential of <i>E. bicyclis</i> to enhance the effectiveness of traditional antibiotics. The findings suggest that <i>E. bicyclis</i> extracts, particularly the EtOAc fraction, could serve as a potent natural resource to counteract antibiotic resistance in aquaculture.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 10","pages":"2112-2117"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11540609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}