Journal of microbiology and biotechnology最新文献_第5页

Establishment of an Apical-Out Organoid Model for Directly Assessing the Function of Postbiotics. 建立用于直接评估益生菌功能的根尖-外生有机体模型

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-09 DOI: 10.4014/jmb.2405.05034

Yeonoh Cho, Moon-Hee Sung, Hee-Taik Kang, Jong Hun Lee

{"title":"Establishment of an Apical-Out Organoid Model for Directly Assessing the Function of Postbiotics.","authors":"Yeonoh Cho, Moon-Hee Sung, Hee-Taik Kang, Jong Hun Lee","doi":"10.4014/jmb.2405.05034","DOIUrl":"10.4014/jmb.2405.05034","url":null,"abstract":"In vitro organoids that mimic the physiological properties of in vivo organs based on three-dimensional cell cultures overcome the limitations of two-dimensional culture systems. However, because the lumen of a typical intestinal organoid is internal, we used an apical-out intestinal organoid model in which the lumen that absorbs nutrients is outside to directly assess the function of postbiotics. A composite culture supernatant of Lactiplantibacillus plantarum KM2 and Bacillus velezensis KMU01 was used as a postbiotic treatment. Expression of COX-2 decreased in apical-out organoids co-treated with a lipopolysaccharide (LPS) and postbiotics. Expression of tight-junction markers such as ZO-1, claudin, and Occludin increased, and expression of mitochondrial homeostasis factors such as PINK1, parkin, and PGC1a also increased. As a result, small and large intestine organoids treated with postbiotics protected tight junctions from LPS-induced damage and maintained mitochondrial homeostasis through mitophagy and mitochondrial biogenesis. This suggests that an apical-out intestinal organoid model can confirm the function of food ingredients.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2184-2191"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peperomia pellucida's Ingredients, Antioxidant Properties, and Safe Usage as Food and Herbal Medicine. Peperomia pellucida 的成分、抗氧化特性以及作为食品和中药的安全使用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-20 DOI: 10.4014/jmb.2406.06025

Chau Thanh Tuan, Tran Thanh Men

{"title":"Peperomia pellucida's Ingredients, Antioxidant Properties, and Safe Usage as Food and Herbal Medicine.","authors":"Chau Thanh Tuan, Tran Thanh Men","doi":"10.4014/jmb.2406.06025","DOIUrl":"10.4014/jmb.2406.06025","url":null,"abstract":"Peperomia pellucida (L.) Kunth has traditionally been used as a vegetable and herbal medicine in certain countries, though its safety remains uncertain. In this study, we investigated the plant's ingredients, volatile compounds, antioxidative activities, and toxicity in Drosophila and mice. The results revealed that P. pellucida contains volatile substances that give it a unique flavor, making it suitable for consumption and rich in antioxidant compounds, such as polyphenols, tannins, flavonoids, saponins, alkaloids, and terpenoids. The total phenolic content (TPC) and total flavonoid content (TFC) of the plant extract were 273.33 ± 4.91 mg gallic acid equivalents/g extract and 199.8 ± 0.346 mg quercetin equivalents/g extract, respectively. The remarkable antioxidative properties of the plant extract were demonstrated by nearly doubling the lifespan of Drosophila against oxidative stress. Moreover, the extract did not cause any acute or chronic toxicity in mice after being fed the plant, as indicated by the normal blood parameters and the absence of hepatic shape damage or impaired function. In light of these findings, P. pellucida is deemed safe for consumption and its bioactive ingredients can be extracted to create functional foods and pharmaceuticals.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2321-2330"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CKAP2 Regulated by TFDP1 Promotes Metastasis and Proliferation of Colorectal Cancer through Affecting the Tumor Microenvironment. 受 TFDP1 调控的 CKAP2 通过影响肿瘤微环境促进结直肠癌的转移和增殖

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-20 DOI: 10.4014/jmb.2407.07008

Zhiqiang Zhong, Shi Cheng, Yang Liu

{"title":"CKAP2 Regulated by TFDP1 Promotes Metastasis and Proliferation of Colorectal Cancer through Affecting the Tumor Microenvironment.","authors":"Zhiqiang Zhong, Shi Cheng, Yang Liu","doi":"10.4014/jmb.2407.07008","DOIUrl":"10.4014/jmb.2407.07008","url":null,"abstract":"The current pathological and physiological evaluation system for colorectal cancer (CRC) is limited; thus, effective biological targets to diagnose and treat this disease are urgently needed. In this study, we used qRT-PCR for detecting mRNA levels of genes. The levels of protein were identified by western blot, immunohistochemistry, and immunofluorescence assays. In addition, functional experiments were used to evaluate the role of cytoskeleton associated protein (CKAP) 2 in CRC cells and human umbilical vein endothelial cells (HUVECs). Bioinformatics analysis was employed to predict the binding relationship of CKAP2 and TFDP1, which was confirmed through dual luciferase reporter assay and immunoprecipitation assay. Furthermore, we injected human colorectal carcinoma HCT116 cells into mice flanks, and we injected Luciferase-labeled HCT116 cells into mice tail vein. HE staining was used to detect tumor nodules. As a result, high CKAP2 expression was found in CRC cells and tissues. CKAP2 silencing reduced CRC cell migration, invasion, proliferation, and epithelial-mesenchymal transition. Moreover, CKAP2 expression was positively associated with M2 macrophage levels. CKAP2 promoted protein expression of CD86, CD206, IL-1β, and CCL17. Moreover, CKAP2 promoted the proliferation of HUVECs and angiogenesis via affecting the tumor microenvironment (TME). We also found that CKAP2 could interact with TFDP1. The inhibitory impacts of TFDP1 downregulation on CRC cell' proliferation, migration, and invasion were reversed via CKAP2 overexpression. In vivo silencing of CKAP2 repressed tumor growth and metastasis. Overall, CKAP2 was positively regulated by TFDP1, which promoted tumorigenesis and metastasis in CRC.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2211-2222"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conductive Bio-Harvesting Tonic (CBT) with an Anti-Dandruff Effect Enhances Hair Growth by Utilizing Naturally Generated Electric Energy during Human Activities. 具有去屑效果的导电生物采集营养剂（CBT）可利用人体活动时自然产生的电能促进头发生长。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-06 DOI: 10.4014/jmb.2408.08014

Jisun Kim, Yoonsuk Lee, Jungbum Kim, Chai Won Park, Hyeonhui Song, Jinkee Hong, Sangmin Lee, Won Hee Jung, Joo-Hyun Hong, Ki Hyun Kim, Wonhwa Lee

{"title":"Conductive Bio-Harvesting Tonic (CBT) with an Anti-Dandruff Effect Enhances Hair Growth by Utilizing Naturally Generated Electric Energy during Human Activities.","authors":"Jisun Kim, Yoonsuk Lee, Jungbum Kim, Chai Won Park, Hyeonhui Song, Jinkee Hong, Sangmin Lee, Won Hee Jung, Joo-Hyun Hong, Ki Hyun Kim, Wonhwa Lee","doi":"10.4014/jmb.2408.08014","DOIUrl":"10.4014/jmb.2408.08014","url":null,"abstract":"Alopecia, while not life-threatening, significantly impacts mental health, identity, and self-esteem of those afflicted. Current pharmacological and surgical treatments often have side effects and are limited in their ability to regenerate hair follicles (HF). Therefore, effective solutions for alopecia remain elusive. We developed an innovative hair tonic capable of stimulating HF regeneration by harnessing abandoned electric energy generated during human activities, such as the frictional electric field from walking and the electric fields from electronic devices. We devised a convenient, non-volatile, and conductive hair tonic to capture these naturally occurring electric fields. We identified 6-pentyl-α-pyrone (6PP) from Trichoderma gamsii as an antifungal agent effective against the dandruff-associated fungus Malassezia that can influence alopecia and adopted it into our conductive bio-harvesting tonic (CBT). Testing on hair follicle dermal papilla cells (HFDPC) and SKH1 mice showed that CBT significantly enhanced HF proliferation and increased growth factors in vitro and in vivo. In SKH1 mice, application of CBT under electric stimulation visibly increased hair shaft length and follicle counts. Additionally, tests on actual human hair follicles demonstrated delayed hair follicle regression when electric stimulation and 6PP were applied. In conclusion, our innovative CBT offers a promising and convenient approach for improving hair growth and combating alopecia.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2376-2384"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Antimicrobial Efficacy of a Designed Synthetic peptide against Pathogenic Bacteria. 评估设计合成肽对致病细菌的抗菌功效

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-08-31 DOI: 10.4014/jmb.2405.05011

Maria Caroline de Moura Cavalheiro, Caio Fernando Ramalho de Oliveira, Ana Paula de Araújo Boleti, Layza Sá Rocha, Ana Cristina Jacobowski, Cibele Nicolaski Pedron, Vani Xavier de Oliveira Júnior, Maria Lígia Rodrigues Macedo

{"title":"Evaluating the Antimicrobial Efficacy of a Designed Synthetic peptide against Pathogenic Bacteria.","authors":"Maria Caroline de Moura Cavalheiro, Caio Fernando Ramalho de Oliveira, Ana Paula de Araújo Boleti, Layza Sá Rocha, Ana Cristina Jacobowski, Cibele Nicolaski Pedron, Vani Xavier de Oliveira Júnior, Maria Lígia Rodrigues Macedo","doi":"10.4014/jmb.2405.05011","DOIUrl":"10.4014/jmb.2405.05011","url":null,"abstract":"Recent research has focused on discovering peptides that effectively target multidrug-resistant bacteria while leaving healthy cells unharmed. In this work, we describe the antimicrobial properties of RK8, a peptide composed of eight amino acid residues. Its activity was tested against multidrug-resistant Gram-negative and Gram-positive bacteria. RK8's efficacy in eradicating mature biofilm and increasing membrane permeability was assessed using Sytox Green. Cytotoxicity assays were conducted both in vitro and in vivo models. Circular dichroism analysis revealed that RK8 adopted an extended structure in water and sodium dodecyl sulfate (SDS). RK8 exhibited MICs of 8-64 μM and MBCs of 4-64 μM against various bacteria, with higher effectiveness observed in Methicillin-resistant Staphylococcus aureus (MRSA) and E. coli KPC+ strains than others. Ciprofloxacin and Vancomycin showed varying MIC and MBC values lower than RK8 for Gram-positive bacteria, but competitive for Gram-negative bacteria. The combination of RK8 and ciprofloxacin showed a synergistic effect. The RK8 peptides could reduce 38% of the mature Acinetobacter baumannii biofilm. Sytox Green reagent achieved 100% membrane permeation of Gram-positive and Gram-negative bacteria. The RK8 peptide did not show cytotoxic effects against murine macrophages (64 μM), erythrocytes (100 μM) or Galleria mellanella larvae (960 μM). In the stability test against peptidases, the RK8 peptide was stable, maintaining around 60% of the molecule intact after 120 min of incubation. These results highlight the potential of RK8 to be a promising strategy for developing a new antimicrobial and antibiofilm agent, inspiring and motivating further research in antimicrobial peptides.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2231-2244"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Throughput Sequencing Analysis of Microbiota and Enzyme Activities in Xiaoqu from Seven Provinces in Southern China. 中国南方七省小曲中微生物群和酶活性的高通量测序分析

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-09 DOI: 10.4014/jmb.2405.05029

Weiwei Dong, Jingjing Zhang, Menglin Zou, Liang Chen, Liping Zhu, Long Zhang, Gang Zhang, Jie Tang, Qiang Yang, Yuanliang Hu, Shenxi Chen

{"title":"High-Throughput Sequencing Analysis of Microbiota and Enzyme Activities in Xiaoqu from Seven Provinces in Southern China.","authors":"Weiwei Dong, Jingjing Zhang, Menglin Zou, Liang Chen, Liping Zhu, Long Zhang, Gang Zhang, Jie Tang, Qiang Yang, Yuanliang Hu, Shenxi Chen","doi":"10.4014/jmb.2405.05029","DOIUrl":"10.4014/jmb.2405.05029","url":null,"abstract":"Xiaoqu, a pivotal starter in baijiu fermentation, provides the most microflora and enzymes to initiate and maintain baijiu brewing. This study aims to explore the differences in microbiota and enzyme activities among Xiaoqu samples from seven provinces in southern China using high-throughput sequencing, plate isolation, and activity detection. The analyses revealed significant differences in bacterial and fungal communities across the samples. A total of 22 bacterial species and 17 target fungal species were isolated and identified. Predominant bacteria included Bacillus (Bacillus subtilis) and lactic acid bacteria (LABs), while the fungal communities were primarily composed of yeasts (Saccharomyces cerevisiae) and various molds. The activities of α-amylase and glucoamylase varied significantly among the samples, and samples from HN1 and GZ2 exhibited the highest activities. Correlation analyses highlighted the pivotal role of LABs in maintaining acidity and the importance of molds and yeasts in the saccharification and fermentation processes. These findings shed light on the microbial composition and diversity of Xiaoqu and the critical role of microbes in baijiu production. Moreover, they suggested potential microbial resources for developing artificial Xiaoqu via synthetic microbial community in the future, enhancing baijiu fermentation efficiency and overall product quality.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2290-2300"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phlorotannin Supplement Improves Scopolamine-Induced Memory Dysfunction by Rescuing Synaptic Damage in Mice. 通过修复小鼠突触损伤，补充绿丹宁改善东莨菪碱诱发的记忆功能障碍

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-13 DOI: 10.4014/jmb.2407.07009

Minji Kim, Haeun Lee, Sangoh Kwon, Seungmok Cho, Min Young Um

{"title":"Phlorotannin Supplement Improves Scopolamine-Induced Memory Dysfunction by Rescuing Synaptic Damage in Mice.","authors":"Minji Kim, Haeun Lee, Sangoh Kwon, Seungmok Cho, Min Young Um","doi":"10.4014/jmb.2407.07009","DOIUrl":"10.4014/jmb.2407.07009","url":null,"abstract":"This study investigated the efficacy of a phlorotannin supplement (PS) in ameliorating scopolamine (SCO)-induced memory deficits in mice, focusing on synaptic function and the underlying molecular mechanisms. Male C57BL/6N mice were divided into six groups and treated with vehicle, donepezil (5 mg/kg body weight, (BW)), or PS (100, 250, or 500 mg/kg BW) for 6 weeks. Behavioral tests were conducted, followed by Golgi staining, immunofluorescence, and immunoblotting to assess synaptic protein expression and signaling pathways. Behavioral tests showed that PS administration significantly improved SCO-induced memory impairment and restored synaptic protein expression (synaptophysin, synapsin1, and postsynaptic density protein 95) in the hippocampus. Additionally, PS enhanced brain-derived neurotrophic factor (BDNF) signaling and activated the extracellular signal-regulated kinase/CAMP response element binding protein (ERK-CREB) pathway, essential for synaptic plasticity. Our findings demonstrate that PS mitigates SCO-induced memory dysfunction by protecting synaptic integrity and activating the BDNF-ERK-CREB signaling pathway, indicating the potential of PS as a natural intervention for treating memory deficits.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2301-2309"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavobacterium nakdongensis sp. nov., Isolated from Fresh Water during the Cyanobacterial Bloom Period. 在蓝藻繁殖期从淡水中分离出的黄杆菌（Flavobacterium nakdongensis sp.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-29 DOI: 10.4014/jmb.2405.05026

Ve Van Le, So-Ra Ko, Sang-Ah Lee, Chi-Yong Ahn

{"title":"Flavobacterium nakdongensis sp. nov., Isolated from Fresh Water during the Cyanobacterial Bloom Period.","authors":"Ve Van Le, So-Ra Ko, Sang-Ah Lee, Chi-Yong Ahn","doi":"10.4014/jmb.2405.05026","DOIUrl":"10.4014/jmb.2405.05026","url":null,"abstract":"A novel Gram-negative bacterial strain, 20NA77.7T, was isolated from fresh water of the Nakdong River. Strain 20NA77.7T shared the highest similarity with Flavobacterium indicum GPTSA100-9T (97.91%) and Flavobacterium urocaniciphilum DSM 27078T (96.24%) in the 16S rRNA gene sequence. The digital DNA-DNA hybridization and average nucleotide identity values for strain 20NA77.7T with Flavobacterium species were below 20.8% and 77.33%, respectively. The major fatty acids of strain 20NA77.7T were identified as iso-C15:0, iso-C16:0, iso-C15:1 G, anteiso-C15:0, iso-C15:0 3OH, and iso-C16:0 3OH. Strain 20NA77.7T contained phosphatidylethanolamine, one unidentified aminolipid, and three unidentified lipids as polar lipids and menaquinone-6 as menaquinone. The polyphasic evidence supports the classification of strain 20NA77.7T as a novel species belonging to the genus Flavobacterium, for which the name Flavobacterium nakdongensis is proposed. The type strain is 20NA77.7T (= KCTC 102000T = LMG 33137T).","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2245-2251"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstitution of the Mevalonate Pathway for Improvement of Isoprenoid Production and Industrial Applicability in Escherichia coli. 重组大肠杆菌中的甲羟戊酸途径，提高异戊烯类化合物的产量和工业应用性。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-10-11 DOI: 10.4014/jmb.2408.08053

Min-Kyoung Kang, Minh Phuong Nguyen, Sang-Hwal Yoon, Keerthi B Jayasundera, Jong-Wook Son, Chonglong Wang, Moonhyuk Kwon, Seon-Won Kim

{"title":"Reconstitution of the Mevalonate Pathway for Improvement of Isoprenoid Production and Industrial Applicability in Escherichia coli.","authors":"Min-Kyoung Kang, Minh Phuong Nguyen, Sang-Hwal Yoon, Keerthi B Jayasundera, Jong-Wook Son, Chonglong Wang, Moonhyuk Kwon, Seon-Won Kim","doi":"10.4014/jmb.2408.08053","DOIUrl":"10.4014/jmb.2408.08053","url":null,"abstract":"Natural products, especially isoprenoids have many industrial applications, including medicine, fragrances, food additives, personal care and cosmetics, colorants, and even advanced biofuels. Recent advancements in metabolic engineering with synthetic biology and systems biology have drawn increased interest in microbial-based isoprenoid production. In order to engineer microorganisms to produce a large amount of value-added isoprenoids, great efforts have been made by employing various strategies from synthetic biology and systems biology. We also have engineered E. coli to produce various isoprenoids by targeting and engineering the isoprenoid biosynthetic pathways, methylerythritol phosphate (MEP), and mevalonate (MVA) pathways. Here, we introduced new combinations of the MVA pathway in E. coli with genes from biosafety level 1 (BSL 1) organisms. The reconstituted MVA pathway constructs (pSCS) are not only preferred to the living modified organism (LMO) regulation, but they also improved carotenoid production. In addition, the pSCS constructs resulted in enhanced lycopene production and cell-specific productivity compared to the previous MVA pathway combination (pSNA) in fed-batch fermentation. The pSCS constructs would not only bring an increase in isoprenoid production in E. coli, but they could be an efficient system to be applied for the industrial production of isoprenoids with industry-preferred genetic combinations.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 12","pages":"2338-2346"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Pigments as a Promising Alternative to Synthetic Colorants: From Fundamentals to Applications. 细菌颜料作为合成着色剂的理想替代品：从基础到应用。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-11-28 Epub Date: 2024-09-11 DOI: 10.4014/jmb.2404.04018

Xin Huang, Longzhan Gan, Zhicheng He, Guangyang Jiang, Tengxia He

{"title":"Bacterial Pigments as a Promising Alternative to Synthetic Colorants: From Fundamentals to Applications.","authors":"Xin Huang, Longzhan Gan, Zhicheng He, Guangyang Jiang, Tengxia He","doi":"10.4014/jmb.2404.04018","DOIUrl":"10.4014/jmb.2404.04018","url":null,"abstract":"Pigments find widespread application in the fields of food, medicine, textiles, and cosmetics. At present, synthetic colorants dominate the global pigment market. However, the environmental and health hazards associated with synthetic colorants have spurred extensive research on eco-friendly and safe alternatives. Natural pigments are particularly intriguing for meeting consumer demands and sustainable development, as they not only exhibit various vibrant color shades without discernible toxic side effects but also offer additional healthful features such as antibacterial, antioxidant, anticancer, and antiviral properties compared with their synthetic counterparts. Among natural sources, bacterial strains share distinct advantages for large-scale pigment production because of their intrinsic robustness of cellular metabolic systems. This review comprehensively outlines the bacterial sources, extraction and purification methods, structural characteristics, biological activities, and potential applications of typical pigments, including but not limited to violacein, indigoidine, melanin, carotenoids, prodigiosin, and rhodopsin. Additionally, it underscores the primary obstacles to the development and production of bacterial pigments for commercial applications, discussing feasible strategies for overcoming production bottlenecks. This work also provides valuable insights for the scientific and rational advancement of bacterial pigment development.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"34 11","pages":"2153-2165"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0