Journal of microbiology and biotechnology最新文献_第5页

The Gut Microbiome in Hepatocellular Carcinoma: Proliferation, Inhibition, Diagnosis, and Immunotherapy. 肝细胞癌的肠道微生物群：增殖、抑制、诊断和免疫治疗。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2412.12075

Moo-Seung Lee, Mi-Young Son, Hyun-Soo Cho

引用次数: 0

Rare and Intermediate Taxa Shape the Gut Bacterial Structure in Neonates and Preterm Infants with Necrotizing Enterocolitis. 罕见和中等分类群塑造坏死性小肠结肠炎新生儿和早产儿肠道细菌结构。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2501.01035

Yu Wang, Qi Wang, Fengan Jia, Dan Li, Xuyang Gao, Xiaoge Zhang, Fan Chang, Yun Xie

{"title":"Rare and Intermediate Taxa Shape the Gut Bacterial Structure in Neonates and Preterm Infants with Necrotizing Enterocolitis.","authors":"Yu Wang, Qi Wang, Fengan Jia, Dan Li, Xuyang Gao, Xiaoge Zhang, Fan Chang, Yun Xie","doi":"10.4014/jmb.2501.01035","DOIUrl":"10.4014/jmb.2501.01035","url":null,"abstract":"Necrotizing enterocolitis (NEC) is a common neonatal gastrointestinal disease with high morbidity and mortality, especially in premature infants. In a prospective case-control study, we aimed to investigate the dynamic changes in the gut microbiota of preterm infants with NEC. Infants diagnosed with NEC and preterm neonates were enrolled in this study, while normal neonates were selected as the control group. The collected samples were divided into three groups: the control group (NC), the neonatal NEC group (NEC), and the premature delivery NEC group (pdNEC). Along with basic clinical data, fecal samples from the infants (n = 39) were collected at the time of the first diagnosis of NEC for 16S rRNA gene sequencing. Analysis of the gut microbiota revealed no significant difference in α-diversity between infants with NEC and controls, regardless of preterm birth. The significant difference in β-diversity was primarily driven by the rare and intermediate subgroups. The rare gut subgroup found in premature infants with NEC played a crucial role in the deterministic process and specialized functionality of the microbiota, ultimately forming a sparse association network structure. Finally, multiple biomarkers of Enterococcus from the Firmicutes phylum were identified, providing a theoretical basis for diagnosing NEC in premature infants.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501035"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

7,7-Bis(3-Indolyl)-p-Cresol, a Metabolite from Marine-Derived Bacterium Vibrio spp. DJA11, Suppresses the Proliferation and Motility of Prostate Cancer Cells. 海洋来源弧菌DJA11的代谢物7,7-双（3-吲哚基）-对甲酚抑制前列腺癌细胞的增殖和运动。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2502.02035

Sultan Pulat, Eun-Young Lee, Grace Choi, Yoon-Hee Jung, Sang-Jip Nam, Hangun Kim

{"title":"7,7-Bis(3-Indolyl)-p-Cresol, a Metabolite from Marine-Derived Bacterium Vibrio spp. DJA11, Suppresses the Proliferation and Motility of Prostate Cancer Cells.","authors":"Sultan Pulat, Eun-Young Lee, Grace Choi, Yoon-Hee Jung, Sang-Jip Nam, Hangun Kim","doi":"10.4014/jmb.2502.02035","DOIUrl":"10.4014/jmb.2502.02035","url":null,"abstract":"Bacteria such as Vibrio spp. in the marine environment can produce secondary metabolites which have significant potential applications in pharmaceuticals. In a study to discover bioactive secondary metabolites from marine Vibrio spp., the strain DJA11 was encountered. HPLC/UV-guided isolation of the crude extract from this strain has led to the discovery of compound 1. Prostate cancer (PCa) is one of the biggest worldwide health issues because of its high diagnosis. CWR22Rv1 (22Rv1) is mutated in WT p53 and AR, C4-2 is derived from androgen-dependent human LNCaP and PC-3 is an androgen-independent cancer cell type. It was found that compound 1 exhibited no significant cytotoxicity at concentrations below 50 μM to human PCa cells, including 22Rv1, C4-2, and PC-3, like normal cell HEK293T. In addition, we presented that 1 inhibited the invasiveness and proliferation of 22Rv1, PC-3, and C4-2 cells by suppressing the activation of p-AKT, p-mTOR, p-STAT3, HSP90, and HSP70. Moreover, treatment with 1 decreased the mRNA expression level of ErbB4, PDK1, STAT3, HSP70, and HSP90 in some PCa cells. Therefore, compound 1 may have therapeutic potential in PCa due to its role in suppressing cancer proliferation and metastasis.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2502035"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of N-Glycan Profiles on Binding Affinity of Diagnostic Antibody Produced by Hybridomas in Serum-Free Suspension. n -聚糖谱对杂交瘤无血清悬浮液中诊断抗体结合亲和力的影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2501.01036

Tae-Ho Kim, Dae Eung Kim, Hoon-Min Lee, Mi-Jung Kang, Jung Hwa Kim, Jungmok You, Mi Kyeong Lee, Yeon-Gu Kim

{"title":"Effect of N-Glycan Profiles on Binding Affinity of Diagnostic Antibody Produced by Hybridomas in Serum-Free Suspension.","authors":"Tae-Ho Kim, Dae Eung Kim, Hoon-Min Lee, Mi-Jung Kang, Jung Hwa Kim, Jungmok You, Mi Kyeong Lee, Yeon-Gu Kim","doi":"10.4014/jmb.2501.01036","DOIUrl":"10.4014/jmb.2501.01036","url":null,"abstract":"Serum-free suspension culture for hybridomas is one of the important key steps for efficient diagnostic antibody production while maintaining protein quality and function. Based on the importance of N-glycan profiles in therapeutic antibody production in mammalian cells, the effect of changes in the N-glycan profiles on the function of diagnostic antibody must also be validated. To investigate the influence of diagnostic antibodies with different N-glycan profiles on the binding affinity with target antigens, four glycosylation regulators, tunicamycin, Bis-Tris, galactose, and N-acetylmannosamine, were administered separately to diagnostic antibody-producing hybridomas cultures. Supplementation with these four glycosylation modulators inhibited glycosylation and increased mannosylation, galactosylation, and sialylation in serum-free suspended hybridomas. In particular, the diagnostic antibody produced from a culture with tunicamycin exhibited a significant increase in the aglycosylated form compared with those without tunicamycin or with other glycosylation modulators. Surprisingly, diagnostic antibody with different N-glycan compositions did not significantly affect binding affinity with the target antigen and even aglycosylated antibodies did not affect binding affinity. Taken together, the results indicate that the change in the N-glycan profile of the diagnostic antibody produced in serum-free suspension hybridomas in an altered culture environment did not significantly affect their biological function, which provides valuable insight for the production and quality control of diagnostic antibody.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501036"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential Bioprocesses for Biovalorization of Shrimp Pond Sludge by Hydrolytic Enzymes-Producing Bacterial Consortia and Photosynthetic Bacteria. 水解产酶菌群和光合细菌对虾池污泥生物增值的顺序生物过程研究。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2501.01042

Chutema Thongsongkaew, Benjamas Cherisilp, Asma Billateh, Wageeporn Maneechote, Sirasit Srinuanpan

{"title":"Sequential Bioprocesses for Biovalorization of Shrimp Pond Sludge by Hydrolytic Enzymes-Producing Bacterial Consortia and Photosynthetic Bacteria.","authors":"Chutema Thongsongkaew, Benjamas Cherisilp, Asma Billateh, Wageeporn Maneechote, Sirasit Srinuanpan","doi":"10.4014/jmb.2501.01042","DOIUrl":"10.4014/jmb.2501.01042","url":null,"abstract":"This study aimed to valorize shrimp pond sludge through sequential bioprocesses using hydrolytic enzyme cocktails produced by bacterial consortia, and photosynthetic bacteria. The production of enzyme cocktails by a co-culture of protease-, amylase-, and lipase-producing bacteria (PAL) was performed in a 5-L stirred tank bioreactor using a low-cost medium. The crude enzyme cocktails were concentrated and used to treat shrimp pond sludge. The addition of enzyme cocktails at 2.0 U/ml based on protease activity led to a reduction of total suspended solids by 40.1% and an increase in soluble chemical oxygen demand (COD) by 3 folds. The solubilized nutrients from shrimp pond sludge in liquid fraction were used as a sole nutrient source to cultivate a newly isolated photosynthetic bacteria (PSB) identified as Rhodocista pekingensis. This PSB was able to grow and achieve a high biomass of 1.30 ± 0.28 g/l and produce value-added bioproducts including aminolevulinic acid (11.77 ± 0.55 μM), carotenoids (166.84 ± 0.03 mg/g dry cell weight), and bacteriochlorophylls (771.47 ± 0.17 mg/g dry cell weight). These results highlight the potential use of enzyme cocktails produced by the co-culture of hydrolytic bacteria to facilitate the biovalorization of aquaculture sludge by PSB and may also greatly contribute to biovalorization of other similar aquaculture wastes into valuable bioproducts.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501042"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Evaluation of a Droplet Digital PCR Assay for the Accurately Detecting the CircHIPK3 in Plasma Samples from Patients with Hepatocellular Carcinoma. 准确检测肝癌患者血浆中CircHIPK3的微滴数字PCR方法的建立与评价

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2412.12048

Yuanye Ji, Ping Tuo, Shun Zhang, Ting Cai, Liyun Fu, Qinzhi Deng, Houdao Fu, Guosheng Gao, Fajiu Wang, Peng Zhu

{"title":"Development and Evaluation of a Droplet Digital PCR Assay for the Accurately Detecting the CircHIPK3 in Plasma Samples from Patients with Hepatocellular Carcinoma.","authors":"Yuanye Ji, Ping Tuo, Shun Zhang, Ting Cai, Liyun Fu, Qinzhi Deng, Houdao Fu, Guosheng Gao, Fajiu Wang, Peng Zhu","doi":"10.4014/jmb.2412.12048","DOIUrl":"10.4014/jmb.2412.12048","url":null,"abstract":"Hepatocellular carcinoma (HCC) is an increasingly prevalent malignant neoplasm on a global scale. Circrna HIPK3 (circHIPK3) has been identified as playing a key role in HCC tumorigenesis and as a novel biomarker. In this study, we aimed at developing a sensitive and accurate method for the detection of circHIPK3 in low load plasma samples using a droplet digital PCR (ddPCR). We designed circHIPK3 gradient primers and probes and optimized the PCR system to improve performance. Then we assessed and compared the linearity and sensitivity of ddPCR and quantitative PCR (qPCR) using the circHIPK3 plasmid DNA as a template. Using these methods, circHIPK3 concentrations were quantitatively determined in 3 cell lines and 40 plasma samples to assess clinical stability. Within the plasmid concentration range of 31-36 copies/μl, the ddPCR exhibited a linear fitting equation of Y = 1.037X-0.1724 with R² value of 0.9940, which surpassed the corresponding R² value of 0.9877 for qPCR. Furthermore, the limit of blank (LOB) and limit of detection (LOD) for ddPCR were determined to be 0.157 copies/μl and 0.594 copies/μl, respectively, which were significantly lower than the LOD of qPCR (5.753 copies/μl). In clinical samples, ddPCR demonstrated a commendable correlation with qPCR, evidenced by a Kappa value of 0.677 (p < 0.05, 95% CI [0.503-0.851]) and an intraclass correlation coefficient (ICC) of 0.903 (95% CI [0.831-0.946]). Notably, ddPCR identified 11 positive samples that qPCR failed to detect. The ddPCR-based circHIPK3 liquid biopsy method emerged as a highly sensitive and accurate approach, lending itself well to clinical applications.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412048"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pinosylvin and Sanguinarine Combination to Enhance Antifungal Activity against Candida albicans. Pinosylvin和Sanguinarine联合增强对白色念珠菌的抗真菌活性。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2412.12055

Yaxuan Yue, Jing Liu, Chen Li, Fengfeng Chen, Cheng Yang, Bingtian Zhao

{"title":"Pinosylvin and Sanguinarine Combination to Enhance Antifungal Activity against Candida albicans.","authors":"Yaxuan Yue, Jing Liu, Chen Li, Fengfeng Chen, Cheng Yang, Bingtian Zhao","doi":"10.4014/jmb.2412.12055","DOIUrl":"10.4014/jmb.2412.12055","url":null,"abstract":"Candida albicans is one of the major sources of fungal infections that can lead to life-threatening systemic infections. However, effective control of C. albicans remains a great challenge. Herein, this study aimed at investigating the antifungal effect of a combination of two natural compounds, pinosylvin (PIN) and sanguinarine (SAN), against C. albicans. In order to investigate the antifungal effect and mechanism of the combination of PIN and SAN, antimicrobial assay, time-kill assay, biofilm formation assay, cell membrane integrity assay, and reactive oxygen species (ROS) assay were performed. The results showed that the combination of PIN and SAN was more effective against C. albicans than PIN or SAN alone. PIN and SAN could jointly inhibit biofilm formation and thus attenuate C. albicans adhesion and colonization ability. PIN mainly targeting the cell membrane while SAN mainly inducing the cell to produce large amounts of ROS. Besides, PIN promoted the entry of SAN into C. albicans. Finally, the hemolysis experiment demonstrated that the combination of PIN and SAN is biocompatible. Taken together, the combination of PIN and SAN enhanced the antifungal effect against C. albicans, which has a broad application prospect in the control of C. albicans.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412055"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synurus deltoides Alleviates Anti-Depressive Like Behavior Dysfunction Induced by Chronic Unpredictable Mild Stress via Stress-Related CRF/TLR Pathway. 三角耳草通过应激相关的CRF/TLR通路缓解慢性不可预测轻度应激诱导的抗抑郁样行为障碍

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2501.01043

Seung Gyum Joo, Jong Min Kim, Hyo Lim Lee, Min Ji Go, Tae Yoon Kim, Ju Hui Kim, Han Su Lee, Hyun Ji Eo, Hyun-Jin Kim, Ho Jin Heo

{"title":"Synurus deltoides Alleviates Anti-Depressive Like Behavior Dysfunction Induced by Chronic Unpredictable Mild Stress via Stress-Related CRF/TLR Pathway.","authors":"Seung Gyum Joo, Jong Min Kim, Hyo Lim Lee, Min Ji Go, Tae Yoon Kim, Ju Hui Kim, Han Su Lee, Hyun Ji Eo, Hyun-Jin Kim, Ho Jin Heo","doi":"10.4014/jmb.2501.01043","DOIUrl":"10.4014/jmb.2501.01043","url":null,"abstract":"This study was aimed at assessing the protective effect of the 80% ethanolic extract of Synurus deltoides (EESD) on chronic unpredictable mild stress (CUMS)-induced depressive-like behavior dysfunction. The bioactive compounds of S. deltoides were identified as quinic acid, chlorogenic acid, rutin, 1,3-dicaffeoylquinic acid, and dicaffeoylsuccinoylquinic acid. EESD and bioactive compounds in EESD significantly protected corticosterone-induced hippocampal cellular death and reactive oxygen species (ROS) contents compared to vitamin C in HT22 cells. By conducting the sucrose preference test, forced swimming test, open field test, and tail suspension test, EESD was found to significantly suppress depression-like behavior. EESD effectively reduced mitochondrial dysfunction by regulating cerebral ROS levels, mitochondrial membrane potential, and ATP contents. EESD showed a considerable regulatory effect by regulating serum stress hormones including corticosterone, norepinephrine, serotonin, 5-hydroxyindoleacetic acid, and melatonin. In addition, EESD significantly suppressed stress-related CRF pathway, inflammatory TLR pathway, and apoptotic signal in cerebral tissues. These results suggest that EESD might be a natural plant substance that improves CUMS-induced behavior abnormality by regulating inflammation and hormonal changes in brain tissue. In the future, additional clinical trials or efficacy evaluations of individual compounds of EESD will be needed to confirm the bioactivity ability and usability of EESD.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2501043"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Main Ingredient of Yinhua Pinggan Granules Combined with Meropenem Alleviated Lung Injury Induced by Multidrug-Resistant Klebsiella pneumoniae via Inhibiting NF-κB Pathway and NLRP3 Inflammasome Activation. 银花平肝颗粒主成分联合美罗培南通过抑制NF-κB通路和NLRP3炎性体激活减轻多药耐药肺炎克雷伯菌肺损伤。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2412.12014

Shengyao Zhang, Haofang Wan, Xiaodan Guan, Daojun Yu, Jiehong Yang, Haitong Wan

{"title":"Main Ingredient of Yinhua Pinggan Granules Combined with Meropenem Alleviated Lung Injury Induced by Multidrug-Resistant Klebsiella pneumoniae via Inhibiting NF-κB Pathway and NLRP3 Inflammasome Activation.","authors":"Shengyao Zhang, Haofang Wan, Xiaodan Guan, Daojun Yu, Jiehong Yang, Haitong Wan","doi":"10.4014/jmb.2412.12014","DOIUrl":"10.4014/jmb.2412.12014","url":null,"abstract":"In combating the global epidemic of multidrug-resistant Klebsiella pneumoniae (MDR-KP), combination therapy with the active ingredient of meropenem (MER) is gaining attention as a new therapeutic approach. In this study, the effect of OAY (orthogonal combination drug of active ingredients in YHPG) in combination with MER on MDR-KP was assessed using the microdilution technique. Additionally, the antimicrobial effect of OAY in combination with MER on MDR-KP was analyzed by reactive oxygen species (ROS), alkaline phosphatase (AKP), and RT-qPCR techniques. Furthermore, the expression levels of critical targets within the NF-κB/NLRP3 pathway were assessed via HE staining and western blot in an MDR-KP-infected mice model. Our results confirmed that the OAY-MER combinations inhibited MDR-KP biofilm formation. In the meantime, the compromise of membrane integrity led to the generation of ROS, which subsequently resulted in a decrease in the activity of intracellular enzymes, specifically AKP. We also found that the combination of OAY-MER reversed tmexCD1-toprJ-mediated MER resistance in MDR-KP. Finally, by a mouse model of MDR-KP infection, the data demonstrated that OAY and YHPG ameliorated lung injury and bacterial infections in the lungs, and significantly reduced NF-κB P-p65, NLRP3, and C-GSDMD protein expression in mouse lung tissues. The findings suggest that the combination of OAY with meropenem may have great potential for clinical application and could provide a theoretical basis for its use in treating MDR-KP infections.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2412014"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Extraction from Chlorella pyrenoidosa Using Bacillus spp. Isolated from Jeotgal: Strain isolation, Characterization, and Fermentation. 利用芽孢杆菌从焦糖小球藻中提取蛋白质：菌株的分离、鉴定和发酵。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2025-05-15 DOI: 10.4014/jmb.2411.11070

Kyung-Jin Cho, Min-Ung Kim, Geum-Jae Jeong, Do Kyung Oh, Ju-Hong Kang, Da-Hyeon Yoon, Fazlurrahman Khan, Young-Mog Kim

{"title":"Protein Extraction from Chlorella pyrenoidosa Using Bacillus spp. Isolated from Jeotgal: Strain isolation, Characterization, and Fermentation.","authors":"Kyung-Jin Cho, Min-Ung Kim, Geum-Jae Jeong, Do Kyung Oh, Ju-Hong Kang, Da-Hyeon Yoon, Fazlurrahman Khan, Young-Mog Kim","doi":"10.4014/jmb.2411.11070","DOIUrl":"10.4014/jmb.2411.11070","url":null,"abstract":"Chlorella pyrenoidosa, a versatile microalga with a rich nutritional profile and functional components, has various applications. However, rigid cell walls pose challenges for the effective extraction of proteins. Microbial fermentation is a promising solution for large-scale production and industrial applications. This study aimed to isolate Bacillus spp. with high enzymatic activity from Jeotgal, a Korean traditional fermented food, and enhance protein extraction from C. pyrenoidosa using microbial fermentation with the isolated Bacillus spp. Twenty-two strains of Bacillus spp. were isolated, and eight Bacillus species were selected based on their ability to produce cellulases, proteases, and lipases. Microbial safety was further assessed by testing for biogenic amine production and hemolytic activity. All eight strains exhibited γ-hemolysis, with four strains not producing biogenic amines. Notably, fermentation using Bacillus amyloliquefaciens F2 showed the highest protein extraction yield at 35.45 ± 1.21% (v/v). In conclusion, this study demonstrates the potential of microbial fermentation for protein extraction from C. pyrenoidosa, offering a novel approach for its utilization in the food industry.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2411070"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0