Journal of microbiology and biotechnology最新文献

{"title":"Assessment of the Risk of Microplastics on Gill and Gut Health and Subsequent Pathogen Susceptibility in the Goldfish Model.","authors":"Ho Sung Kim, Bohyun Yun, Yongjoon Yoon, Jeong Woo Park, Jimin Hyun, BoMi Ryu, Aaron M Yerke, Sungmin Hwang, Ki Hwan Moon","doi":"10.4014/jmb.2504.04019","DOIUrl":"10.4014/jmb.2504.04019","url":null,"abstract":"<p><p>Microplastics are pervasive pollutants in aquatic ecosystems, yet their effects on fish tissues remain insufficiently characterized. Our study investigates the impact of polystyrene microplastics (0.5 and 2 μm) on the gill and intestinal tissues of goldfish (<i>Carassius auratus</i>), with a focus on inflammatory responses and pathogen susceptibility. Following two weeks of exposure, histological and molecular analyses revealed increased filament cartilage thickness in gills, enhanced villus thickness and goblet cell numbers in intestines, and upregulation of immune- and oxidative stress-related genes. Exposure to 0.5 μm microplastics significantly reduced survival after <i>Edwardsiella piscicida</i> infection, indicating increased vulnerability to pathogens. These findings highlight the immunotoxic effects of microplastics and their potential to compromise fish health in contaminated environments.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504019"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LOR Regulated by METTL3 Alleviates Lipopolysaccharides-Induced Periodontitis Injury.","authors":"Qin Su, Jiao Chen","doi":"10.4014/jmb.2505.05016","DOIUrl":"10.4014/jmb.2505.05016","url":null,"abstract":"<p><p>Periodontitis is a chronic inflammatory disease-causing tissue destruction and systemic effects. Despite significant advancements, the molecular mechanisms driving tissue degeneration remain incompletely understood. Emerging evidence suggests that RNA modifications, particularly N6-methyladenosine (m⁶A) methylation, critically regulate inflammatory responses. This study investigates the role of METTL3-mediated m⁶A modification of loricrin (LOR) in lipopolysaccharide (LPS)-induced periodontal injury. Bioinformatics analyses identified the key downregulated gene in periodontitis. To establish an <i>in vitro</i> periodontitis model, human periodontal ligament fibroblast (HPLF) cells were treated with LPS. LOR and METTL3 levels in clinical samples and HPLF cells were measured by qRT-PCR. Inflammatory cytokines, cell proliferation, and apoptosis were examined using ELISA, CCK8, EdU, and flow cytometry assays, respectively. The interaction between LOR and METTL3 was evaluated through Pearson correlation, MeRIP assay, qRT-PCR, immunoblotting, and mRNA stability assays. LOR was identified as a key downregulated gene in periodontitis, as validated in both clinical tissues and a periodontitis cell model. Functional assays showed that LPS-treatment promoted inflammatory cytokine production, inhibited cell proliferation, and increased apoptosis, whereas upregulating LOR in these cells reversed these effects. Furthermore, METTL3 expression was reduced in periodontitis clinical tissues and positively correlated with LOR expression. METTL3 overexpression enhanced LOR mRNA stability via m⁶A methylation. Moreover, silencing METTL3 partially negated the protective effects of LOR overexpression in LPS-induced periodontitis cell model. These findings reveal that METTL3-mediated m⁶A modification of LOR mitigates periodontal injury, suggesting that the METTL3-LOR axis may represent a potential avenue for future therapeutic exploration to maintain periodontal homeostasis.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505016"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indole Propionic Acid Regulates Gut Immunity: Mechanisms of Metabolite-Driven Immunomodulation and Barrier Integrity.","authors":"Tao Ren, Dihao Li, Feng Sun, Lijia Pan, Ao Wang, Xinze Li, Yuwen Bao, Meiyu Zhang, Fei Zheng, Hao Yue","doi":"10.4014/jmb.2503.03045","DOIUrl":"10.4014/jmb.2503.03045","url":null,"abstract":"<p><p>Indole propionic acid (IPA) is a functional indole derivative produced exclusively by intestinal flora through tryptophan metabolism. Numerous studies have shown that IPA has a variety of beneficial biological functions, including anti-inflammatory and antioxidant effects, immunomodulation, intestinal barrier protection, regulation of intestinal flora composition, and neuroprotection. IPA, as an intestinal microbial metabolite, actively participates in the establishment of intestinal immune homeostasis and positively influences the prevention and control of intestinal diseases, thereby playing an indispensable role in regulating host health. We conducted a comprehensive literature review to explore the synthesis of IPA <i>in vivo</i>, the mechanism of action on intestinal immunity, and the promise of its application in the treatment of related diseases. The physiological and biological effects of IPA were investigated to explore its potential application in future drug discovery. Obviously, IPA plays an important role in intestinal immunity and is effective in the treatment of related diseases. IPA helps regulate intestinal immune cell function, inhibiting inflammatory response and enhancing intestinal barrier function through its effects on the aryl hydrocarbon receptor, the pregnane X receptor, and other related signaling pathways. The development of IPA as a target drug for the treatment of intestinal diseases is promising. Although IPA research is still in the experimental animal model stage, there is growing interest in the many therapeutic applications of IPA and increasing opportunities to further modify IPA for future clinical applications.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503045"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"8-Methoxybicolosin C from <i>Lespedeza bicolor</i> Attenuates Inflammation and Oxidative Stress via Nrf2/HO-1 and NF-κB/MAPK Pathways in Lipopolysaccharide-Induced Mouse Kupffer Cells.","authors":"Young-Chang Cho, Lulu Yao, Da Young Lee, Xiangying Li, Guijae Yoo, Sang Yoon Choi, Namki Cho, Su-Jin Park, Somy Yoon, Jae Sung Lim","doi":"10.4014/jmb.2503.03013","DOIUrl":"10.4014/jmb.2503.03013","url":null,"abstract":"<p><p><i>Lespedeza bicolor</i> (<i>L. bicolor</i>) is known for its anti-inflammatory, antioxidant, and anticancer properties, making it a common choice in traditional medicine practices. Researchers in several recent studies have focused on isolating individual phytochemicals from this plant through chromatography analysis to explore their therapeutic potential. In our previous work, we identified 8-methoxybicolosin C (8-MC) as a novel flavonoid derivative, isolated and purified from the roots of <i>L. bicolor</i>, which exhibited inhibitory effects on cell proliferation. In this study, we further investigated the biological activities of 8-MC by examining its antioxidant and anti-inflammatory effects in LPS-induced mouse Kupffer cells. The results showed that 8-MC suppresses the expression of inflammation-related mediators, including inducible nitric oxide synthase (iNOS), nitric oxide (NO), and pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β, in a dose-dependent manner. Additionally, 8-MC improves the GSH/GSSG balance by increasing glutathione (GSH) levels and decreasing oxidized glutathione (GSSG) levels. Interestingly, 8-MC was found to bind Keap1, preventing roteasomal degradation, and promoting the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), thereby increasing the expression of antioxidant-related proteins such as heme oxygenase-1 (HO-1). Moreover, 8-MC suppressed the activation of inflammatory signaling pathways, including c-Jun N-terminal kinases (JNKs) and p38 mitogen-activated protein kinases (MAPKs), while also inhibiting the nuclear translocation of nuclear factor kappa B (NF-κB), effectively reducing inflammatory responses. These findings collectively demonstrated that 8-MC possesses potent anti-inflammatory and antioxidant activities through the regulation of NF-κB, MAPK, and Nrf2/HO-1 signaling pathways. Consequently, 8-MC shows potential as a valuable therapeutic agent for managing various inflammatory disorders.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503013"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Domestic Alternative Positive Control Strain to <i>Bacillus cereus</i> ATCC 14579 for Microbial Assays.","authors":"Su-Hyeon Joung, Yu-Si Lee, Byeong Joon Kim, Yongchjun Park, Seung Hwan Kim, Soon Han Kim, Insun Joo, Eun Sook An","doi":"10.4014/jmb.2504.04003","DOIUrl":"10.4014/jmb.2504.04003","url":null,"abstract":"<p><p>Most positive control strains in microbial assays are sourced from abroad. <i>Bacillus cereus</i> ATCC 14579 is used as the positive control strain in microbial assays described in the Manual for the Detection of Foodborne Pathogens at Outbreaks and the FDA's Bacteriological Analytical Manual method. This study aimed to identify a domestically isolated strain from the Ministry of Food and Drug Safety (MFDS) to replace <i>B. cereus</i> ATCC 14579. A total of 323 strains from the MFDS were collected, and gene-targeted polymerase chain reaction and biochemical tests were performed to characterize candidate strains. Sixty-one candidate strains showing identical biochemical and molecular characteristics to ATCC 14579 were further subjected to whole-genome sequencing. Comparative genetic analyses using MLST, SNP distance, and OrthoANI revealed that <i>B. cereus</i> MFDS 1004972 shared the same sequence type, exhibited SNP distance of fewer than 10 SNPs, and showed 100% ANI with ATCC 14579. Therefore, <i>B. cereus</i> MFDS 1004972 is proposed as a suitable domestic alternative to the imported strain.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504003"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review on Current Research on Biosynthesis of Biosurfactants and the Regulation Influenced by Metal Ions.","authors":"Yuchen Wang, Shan Qiao, Yongwu Niu","doi":"10.4014/jmb.2503.03031","DOIUrl":"10.4014/jmb.2503.03031","url":null,"abstract":"<p><p>Biosurfactants, amphiphilic secondary metabolites mainly produced by microorganisms, can be categorized into five groups according to their chemical structure and source: glycolipids, lipopeptides, phospholipids, polymeric biosurfactants, and particulate surfactants. The hydrophobic segments of biosurfactants typically contain fatty acids of varying chain lengths, while their hydrophilic portions display a wide range of diversity. Diverse biosurfactants have distinct metabolic pathways. Glycolipids are usually associated with glycolysis/gluconeogenesis and fatty acid metabolism, while lipopeptides are closely connected to non-ribosomal peptide synthetase. Particulate biosurfactants are formed through the anabolic pathway of phospholipids, with amino acid metabolism and carbohydrate metabolism being crucial components in the process of creating polar head groups. Metal ions are critical for either directly influencing strain growth or governing genes connected to biosurfactants production. This review explores the synthesis pathways of various biosurfactants and examines the influence of different metal ions on their production.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2503031"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of Gut Microbiota by Red Ginseng Extract Powder and Dietary Fiber in Obese Mice: Identification of Key Microbial Candidates.","authors":"Jonghyeok Shin, Do Young Jin, Seung-Hwan Seo, Hye-Young Yu, Sang-Kyu Kim, Seung-Ho Lee, Eun-Hee Shin, Jun-Seob Kim","doi":"10.4014/jmb.2506.06016","DOIUrl":"10.4014/jmb.2506.06016","url":null,"abstract":"<p><p>Red ginseng extract powder (RGEP) and red ginseng dietary fiber (RGDF) contain bioactive components with potential prebiotic effects. As gut microbiota plays a critical role in obesity and is influenced by prebiotics, we investigated the effects of RGEP and RGDF supplementation on gut microbiota diversity, composition, and metabolic functions in diet-induced obese mice. RGEP and RGDF supplementation altered gut microbiota composition, increasing beneficial bacteria such as <i>Lactobacillus</i>, <i>Roseburia</i>, and <i>Akkermansia</i>. Alpha diversity analysis showed an increase in microbial richness, particularly in the high-dose RGDF group, whereas beta diversity analysis confirmed a distinct separation between red ginseng-fed groups and obesity models. Functional pathway analysis revealed that supplementation with RGEP and RGDF enhanced short-chain fatty acid (SCFA) metabolism, lipid metabolism, and anti-inflammatory metabolism, suggesting modulation of gut microbial functional profiles. These findings suggest that RGEP and RGDF contribute to gut microbiota modulation by enhancing microbial diversity, promoting SCFA metabolism, and suppressing pro-inflammatory bacterial taxa. While only gut microbiota profiles were analyzed, the observed restoration of microbial balance suggests a potential contribution of red ginseng components to host metabolic health, which warrants further investigation. Further studies are needed to validate these findings in human trials and elucidate the underlying molecular mechanisms.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506016"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Drug Candidate against <i>Mycobacterium avium</i> Using Pandemic Response Box.","authors":"Seunghyeon Jeon, Yubin Lee, Jihyeon Yun, Bo Eun Heo, Anwesha Ash, Cheol Moon, Chul-Su Yang, Jichan Jang","doi":"10.4014/jmb.2506.06006","DOIUrl":"10.4014/jmb.2506.06006","url":null,"abstract":"<p><p>MAC (<i>Mycobacterium avium</i> complex) is a naturally occurring environmental microorganism found worldwide in sources such as soil and water. Among nontuberculous mycobacteria (NTM), MAC is the species most commonly responsible for pulmonary infections, particularly in immunocompromised individuals. In addition to pulmonary disease, extrapulmonary <i>M. avium</i> infections can present as disseminated, cutaneous, or lymphatic diseases. Skin infections caused by <i>M. avium</i> can vary significantly between patients, with both localized and disseminated forms observed. Despite the increasing prevalence of extrapulmonary NTM infections, treatment outcomes remain suboptimal, underscoring the need for novel therapeutic strategies. In this study, we conducted in vitro dual-screening using the Pandemic Response Box against <i>M. avium</i> 104, and identified alexidine (AX) as a promising candidate for therapy. Further evaluation demonstrated that chlorhexidine (CHX), a structurally distinct bis-biguanide compound, also exhibited potent inhibitory activity against <i>M. avium</i> growth in vitro, as well as in a zebrafish model of <i>M. avium</i> infection and treatment. These findings suggest that CHX may be a potential therapeutic candidate for treating <i>M. avium</i> skin infections.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506006"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apoptosis-Inducing Effects of <i>Lactobacillus plantarum</i> DS0709 in Colorectal Cancer.","authors":"In Hwan Tae, Yunsang Kang, Jinkwon Lee, Jeongmin Lee, Jinsan Kim, Haneol Yang, Kunhyang Park, Doo-Sang Park, Dae-Soo Kim, Hyun-Soo Cho","doi":"10.4014/jmb.2504.04042","DOIUrl":"10.4014/jmb.2504.04042","url":null,"abstract":"<p><p>In colorectal cancer (CRC) treatment, various approaches, including chemotherapy (5-FU, irinotecan, and oxaliplatin), targeted therapy (VEGF inhibitor) and immunotherapy (PD-1/ PD-L1 inhibitor), are employed. However, due to side effects and limited efficacy, more effective novel therapeutic strategies have been required. In this study, we identified the anti-cancer effects of <i>Lactobacillus plantarum</i> DS0709, isolated from infant feces, on CRC. Treatment with the supernatant (Sup) of <i>L. plantarum</i> DS0709 demonstrated growth inhibition of CRC cell lines (HCT116 and SNUC5) by inducing apoptosis. Additionally, using human iPSC-derived intestinal organoids (hIO), we confirmed that <i>L. plantarum</i> DS0709 Sup exhibited no toxicity. Furthermore, in a 3D spheroid model mimicking <i>in vivo</i> conditions, <i>L. plantarum</i> DS0709 Sup showed similar apoptosis induction and growth-inhibitory effects as in 2D cultures. Thus, these findings suggest that <i>L. plantarum</i> DS0709 has the potential to be developed into a novel microbiome-based therapeutic agent for CRC, offering anti-cancer efficacy without side effects.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2504042"},"PeriodicalIF":3.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refining Dendritic Cell-Based Cancer Vaccines: Subset Targeting, Translational Barriers, and Emerging Strategies.","authors":"Inmoo Rhee","doi":"10.4014/jmb.2506.06021","DOIUrl":"10.4014/jmb.2506.06021","url":null,"abstract":"<p><p>Dendritic cells (DCs) are pivotal regulators of immune responses, capable of initiating robust adaptive immunity through antigen presentation. As the most potent antigen-presenting cells, they have emerged as central components of cancer immunotherapy. Over the last decade, advances in molecular engineering, bioinformatics, and nanomedicine have transformed the design of DC-based vaccines. Strategies now include personalized neoantigen loading, mRNA-electroporation, nanoparticle-mediated delivery, and combinatorial regimens with immune checkpoint inhibitors. In addition, emerging approaches that target DC subsets <i>in vivo</i>, especially cDC1s, have demonstrated enhanced efficacy in preclinical and early clinical studies. This review provides a comprehensive overview of the biological roles of DCs and evaluates the evolution of DC vaccine platforms while also highlighting new technologies and clinical insights that aim to tumor-induced immunosuppression suppression and improve therapeutic outcomes.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2506021"},"PeriodicalIF":3.1,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}