Journal of microbiology and biotechnology最新文献_第5页

Study on Optimization of Liquid Fermentation Medium and Antitumor Activity of the Mycelium on Phyllopora lonicerae. 液体发酵培养基的优化及菌丝体对忍冬疫霉的抗肿瘤活性研究

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-24 DOI: 10.4014/jmb.2405.05004

Min Liu, Lu Liu, Guoli Zhang, Guangyuan Wang, Ranran Hou, Yinghao Zhang, Xuemei Tian

{"title":"Study on Optimization of Liquid Fermentation Medium and Antitumor Activity of the Mycelium on Phyllopora lonicerae.","authors":"Min Liu, Lu Liu, Guoli Zhang, Guangyuan Wang, Ranran Hou, Yinghao Zhang, Xuemei Tian","doi":"10.4014/jmb.2405.05004","DOIUrl":"10.4014/jmb.2405.05004","url":null,"abstract":"Phylloporia lonicerae is an annual fungus that specifically parasitizes living Lonicera plants, offering significant potential for developing new resource food and medicine. However, wild resources and mycelium production of this fungus is limited, and its anti-tumor active ingredients and mechanisms remain unclear, hampering the development of this fungus. Thus, we optimized the fermentation medium of P. lonicerae and studied the anti-tumor activity of its mycelium. The results indicated that the optimum fermentation medium consisted of 2% sucrose, 0.2% peptone, 0.1% KH2PO4, 0.05% MgSO4·7H2O, 0.16% Lonicera japonica petals, 0.18% P fungal elicitor, and 0.21% L. japonica stem. The biomass reached 7.82 ± 0.41 g/l after 15 days of cultivation in the optimized medium, a 142% increase compared with the potato dextrose broth medium, with a 64% reduction in cultivation time. The intracellular alcohol extract had a higher inhibitory effect on A549 and Eca-109 cells than the intracellular water extract, with half-maximal inhibitory concentration values of 2.42 and 2.92 mg/ml, respectively. Graded extraction of the alcohol extract yielded petroleum ether phase, chloroform phase, ethyl acetate phase, and n-butanol phase. Among them, the petroleum ether phase exhibited a better effect than the positive control, with a half-maximal inhibitory concentration of 113.3 μg/ml. Flow cytometry analysis indicated that petroleum ether components could induce apoptosis of Eca-109 cells, suggesting that this extracted component can be utilized as an anticancer agent in functional foods. This study offers valuable technical support and a theoretical foundation for promoting the comprehensive development and efficient utilization of P. lonicerae.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing the Effect of Microbial Consortia Fermentation on the Quality of HnB by Untargeted Metabolomics. 通过非靶向代谢组学分析微生物联合发酵对 HnB 质量的影响

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-30 DOI: 10.4014/jmb.2402.02039

Ling Zou, Hong Zhang, Zhonghua Liu, Jianfeng Sun, Yang Hu, Yishu Ding, Xinwei Ji, Zhenfei Yang, Qi Zhang, Binbin Hu

{"title":"Analyzing the Effect of Microbial Consortia Fermentation on the Quality of HnB by Untargeted Metabolomics.","authors":"Ling Zou, Hong Zhang, Zhonghua Liu, Jianfeng Sun, Yang Hu, Yishu Ding, Xinwei Ji, Zhenfei Yang, Qi Zhang, Binbin Hu","doi":"10.4014/jmb.2402.02039","DOIUrl":"10.4014/jmb.2402.02039","url":null,"abstract":"Fermentation has been identified as an effective strategy to alter the chemical makeup of tobacco, thereby enhancing its quality. The deliberate introduction of microorganisms can hasten the fermentation process. In this research, microbial consortia harvested from the tobacco surface were utilized to enhance the tobacco quality. This enhancement also elevated several sensory attributes of HnB cigarettes, such as aroma richness, moisture, strength, and reduced irritation, achieving a sensory quality rating of 84.5. This marks a notable improvement compared to the 82 rating of the original, unfermented cigarettes. Untargeted metabolomics analysis revealed a decrease in total polyphenols and unsaturated fatty acids, while the levels of polyacids, alcohols, ketones, furans, and other compounds increased in the fermented tobacco. Additionally, KEGG pathway enrichment analysis indicated that the enhancement in tobacco quality through microbial consortia fermentation is linked to various biological pathways, with pathways related to fatty acid and amino acid degradation playing pivotal roles. The findings of this study will serve as a reference for the commercial production of HnB cigarettes, and the elucidated mechanism offers a theoretical basis for exploring microbial fermentation as a means to improve tobacco quality.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Gut Microbial Lipid Metabolite 14(15)-EpETE Inhibits Substance P Release by Targeting GCG/PKA Signaling to Relieve Cisplatin-Induced Nausea and Vomiting in Rats. 肠道微生物脂质代谢物 14(15)- EpETE 通过靶向 GCG/PKA 信号抑制物质 P 的释放，从而缓解大鼠由顺铂引起的恶心和呕吐。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-15 DOI: 10.4014/jmb.2403.03044

Man Lu, Liwei Xie, Sijie Yin, Jing Zhou, Lingmei Yi, Ling Ye

{"title":"The Gut Microbial Lipid Metabolite 14(15)-EpETE Inhibits Substance P Release by Targeting GCG/PKA Signaling to Relieve Cisplatin-Induced Nausea and Vomiting in Rats.","authors":"Man Lu, Liwei Xie, Sijie Yin, Jing Zhou, Lingmei Yi, Ling Ye","doi":"10.4014/jmb.2403.03044","DOIUrl":"10.4014/jmb.2403.03044","url":null,"abstract":"Chemotherapy-induced nausea and vomiting (CINV) is a debilitating side effect related to activation of substance P (SP). SP activation can result from dysregulation of the gut-brain axis, and also from activation of protein kinase A signaling (PKA) signaling. In this study, we connected these factors in an attempt to unveil the mechanisms underlying CINV and develop new therapeutic strategies. Female rats were injected with cisplatin (Cis) to induce pica. Fecal samples were collected before/after injection, and subjected to lipid metabolomics analysis. In another portion of pica rats, the PKA inhibitor KT5720 was applied to investigate the involvement of PKA signaling in CINV, while fecal microbiota transplantation (FMT) was implemented to verify the therapeutic effect of the lipid metabolite 14(15)-EpETE. Pica symptoms were recorded, followed by ileal histological examination. The targeting relationship between 14(15)-EpETE and glucagon was determined by bioinformatics. SP and glucagon/PKA signaling in rat ileum, serum, and/or brain substantia nigra were detected by immunohistochemistry, enzyme-linked immunosorbent assay, and/or western blot. The results showed a significantly lower level of 14(15)-EpETE in rat feces after Cis injection. KT5720 treatment alleviated Cis-induced pica symptoms, ileal injury, SP content increase in the ileum, serum, and brain substantia nigra, and ileal PKA activation in rats. The ileal level of glucagon was elevated by Cis in rats. FMT exerted an effect similar to that of KT5720 treatment, relieving the Cis-induced changes, including ileal glucagon/PKA activation in rats. Our findings demonstrate that FMT restores 14(15)-EpETE production, which inhibits SP release by targeting GCG/PKA signaling, ultimately mitigating CINV.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Characterization of an R-M System in Paracoccus denitrifican DYTN-1 to Improve the Plasmid Conjugation Transfer Efficiency. 鉴定和表征反硝化细菌 DYTN-1 中的 R-M 系统以提高质粒共轭转移效率

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-26 DOI: 10.4014/jmb.2402.02041

Yunpeng Shi, Wenyan Cao, Zhiping Zheng, Sha Xu, Lijuan Chai, Shenghu Zhou, Yu Deng

{"title":"Identification and Characterization of an R-M System in Paracoccus denitrifican DYTN-1 to Improve the Plasmid Conjugation Transfer Efficiency.","authors":"Yunpeng Shi, Wenyan Cao, Zhiping Zheng, Sha Xu, Lijuan Chai, Shenghu Zhou, Yu Deng","doi":"10.4014/jmb.2402.02041","DOIUrl":"10.4014/jmb.2402.02041","url":null,"abstract":"Paracoccus denitrificans has been identified as a representative strain with heterotrophic nitrification-aerobic denitrification capabilities (HN-AD), and demonstrates strong denitrification proficiency. Previously, we isolated the DYTN-1 strain from activated sludge, and it has showcased remarkable nitrogen removal abilities and genetic editability, which positions P. denitrificans DYTN-1 as a promising chassis cell for synthetic biology engineering, with versatile pollutant degradation capabilities. However, the strain's low stability in plasmid conjugation transfer efficiency (PCTE) hampers gene editing efficacy, and is attributed to its restriction modification system (R-M system). To overcome this limitation, we characterized the R-M system in P. denitrificans DYTN-1 and identified a DNA endonuclease and 13 DNA methylases, with the DNA endonuclease identified as HNH endonuclease. Subsequently, we developed a plasmid artificial modification approach to enhance conjugation transfer efficiency, which resulted in a remarkable 44-fold improvement in single colony production. This was accompanied by an increase in the frequency of positive colonies from 33.3% to 100%. Simultaneously, we cloned, expressed, and characterized the speculative HNH endonuclease capable of degrading unmethylated DNA at 30°C without specific cutting site preference. Notably, the impact of DNA methylase M9 modification on the plasmid was discovered, significantly impeding the cutting efficiency of the HNH endonuclease. This revelation unveils a novel R-M system in P. denitrificans and sheds light on protective mechanisms employed against exogenous DNA invasion. These findings pave the way for future engineering endeavors aimed at enhancing the DNA editability of P. denitrificans.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Insights into Cr(VI)-Induced Rhamnolipid Production and Gene Expression in Pseudomonas aeruginosa RW9 for Potential Bioremediation. 铜绿假单胞菌 RW9 中六价铬诱导的鼠李糖脂生产和基因表达的新见解，以实现潜在的生物修复。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-19 DOI: 10.4014/jmb.2406.06034

Fatini Mat Arisah, Norhayati Ramli, Hidayah Ariffin, Toshinari Maeda, Mohammed Abdillah Ahmad Farid, Mohd Zulkhairi Mohd Yusoff

{"title":"Novel Insights into Cr(VI)-Induced Rhamnolipid Production and Gene Expression in Pseudomonas aeruginosa RW9 for Potential Bioremediation.","authors":"Fatini Mat Arisah, Norhayati Ramli, Hidayah Ariffin, Toshinari Maeda, Mohammed Abdillah Ahmad Farid, Mohd Zulkhairi Mohd Yusoff","doi":"10.4014/jmb.2406.06034","DOIUrl":"10.4014/jmb.2406.06034","url":null,"abstract":"Rhamnolipid (RL) is renowned for its efficacy in bioremediating several types of organic and metal contaminants. Nevertheless, there has been a scarcity of studies specifically examining the relationship between this substance and metals, especially in terms of their impact on RL formation and the underlying interaction processes. This study addresses this gap by investigating the RL mechanism in Cr (VI) remediation and evaluating its effect on RL production in Pseudomonas aeruginosa RW9. In this study, P. aeruginosa RW9 was grown in the presence of 10 mg l-1 Cr (VI). We monitored RL yield, congeners distribution, and their ratios, as well as the transcriptional expression of the RL-encoded genes: rhlA, rhlB, and rhlC. Our results revealed that RL effectively reduced Cr (VI) to Cr (III), with RL yield increasing threefold, although with a slight delay in synthesis compared to control cells. Furthermore, Cr (VI) exposure induced the transcriptional expression of the targeted genes, leading to a significant increase in di-RL production. The findings confirm that Cr (VI) significantly impacts RL production, altering its structural compositions and enhancing the transcriptional expression of RL-encoded genes in P. aeruginosa RW9. This study represents a novel exploration of Cr (VI)'s influence on RL production, providing valuable insights into the biochemical pathways involved and supporting the potential of RL in Cr (VI) bioremediation.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ICP5249 Promotes Hair Growth by Activating the AMPK-Autophagy Signaling Pathway. ICP5249通过激活AMPK-自噬信号通路促进毛发生长

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-26 DOI: 10.4014/jmb.2406.06015

Jung Ok Lee, Yu-Jin Kim, You Na Jang, Jung Min Lee, Kayoung Shin, Sekyoo Jeong, Hwa-Jee Chung, Beom Joon Kim

引用次数: 0

Variations in the Antivirulence Effects of Fatty Acids and Virstatin against Vibrio cholerae Strains. 脂肪酸和Virstatin对霍乱弧菌株的抗病毒作用的变化

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-19 DOI: 10.4014/jmb.2405.05002

Donghyun Lee, Jayun Joo, Hunseok Choi, Seonghyeon Son, Jonghyun Bae, Dong Wook Kim, Eun Jin Kim

{"title":"Variations in the Antivirulence Effects of Fatty Acids and Virstatin against Vibrio cholerae Strains.","authors":"Donghyun Lee, Jayun Joo, Hunseok Choi, Seonghyeon Son, Jonghyun Bae, Dong Wook Kim, Eun Jin Kim","doi":"10.4014/jmb.2405.05002","DOIUrl":"10.4014/jmb.2405.05002","url":null,"abstract":"The expression of two major virulence factors of Vibrio cholerae, cholera toxin (CT) and toxin co-regulated pilus (TCP), is induced by environmental stimuli through a cascade of interactions among regulatory proteins known as the ToxR regulon when the bacteria reach the human small intestine. ToxT is produced via the ToxR regulon and acts as the direct transcriptional activator of CT (ctxAB), TCP (tcp gene cluster), and other virulence genes. Unsaturated fatty acids (UFAs) and several small-molecule inhibitors of ToxT have been developed as antivirulence agents against V. cholerae. This study reports the inhibitory effects of fatty acids and virstatin (a small-molecule inhibitor of ToxT) on the transcriptional activation functions of ToxT in isogenic derivatives of V. cholerae strains containing various toxT alleles. The fatty acids and virstatin had discrete effects depending on the ToxT allele (different by 2 amino acids), V. cholerae strain, and culture conditions, indicating that V. cholerae strains could overcome the effects of UFAs and small-molecule inhibitors by acquiring point mutations in toxT. Our results suggest that small-molecule inhibitors should be examined thoroughly against various V. cholerae strains and toxT alleles during development.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitive and Enzyme-Free Pseudomonas aeruginosa Detection and Isolation via DNAzyme Cascade Triggered DNA Tweezer. 通过 DNA 酶级联触发 DNA 镊子灵敏、无酶地检测和分离铜绿假单胞菌。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-08-09 DOI: 10.4014/jmb.2407.07006

Furong Liu, Jingyuan Xu, Lihua Yang

{"title":"Sensitive and Enzyme-Free Pseudomonas aeruginosa Detection and Isolation via DNAzyme Cascade Triggered DNA Tweezer.","authors":"Furong Liu, Jingyuan Xu, Lihua Yang","doi":"10.4014/jmb.2407.07006","DOIUrl":"10.4014/jmb.2407.07006","url":null,"abstract":"Effective isolation and sensitive detection of Pseudomonas aeruginosa (P. aeruginosa) is crucial for the early diagnosis and prognosis of various diseases, such as urinary tract infections. However, efficient isolation and simultaneous detection of P. aeruginosa remains a huge challenge. Herein, we depict a novel fluorescence assay for sensitive, enzyme-free detection of P. aeruginosa by integrating DNAzyme cascade-induced DNA tweezers and magnetic nanoparticles (MNPs)-based separation. The capture probe@MNPs is capable of accurately identifying target bacteria and transporting the bacteria signal to nucleic acid signals. Based on the DNAzyme cascade-induced DNA tweezers, the nucleic acid signals are extensively amplified, endowing the method with a high sensitivity and a low detection limit of 1 cfu/mL. In addition, the method also exhibits a wide detection of six orders of magnitudes. The proposed method could be extended to other bacteria detection by simply changing the aptamer sequence. Taking the merit of the high sensitivity, greatly minimized detection time (less than 1.5 h), enzyme-free characteristics, and stability, the proposed method could be potentially applied to diagnosing and preventing diseases caused by pathogenic bacteria.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesorhizobium koreense sp. nov., Isolated from Soil. Mesorhizobium koreense sp.

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-06-24 DOI: 10.4014/jmb.2404.04026

Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim

{"title":"Mesorhizobium koreense sp. nov., Isolated from Soil.","authors":"Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim","doi":"10.4014/jmb.2404.04026","DOIUrl":"10.4014/jmb.2404.04026","url":null,"abstract":"An aerobic, Gram-stain-negative, catalase-positive, rod-shaped, and motile bacteria, designated as a strain WR6T was isolated from soil in Republic of Korea. Strain WR6T grew at temperatures of 10-37°C, at pH of 5.0-9.0, and at NaCl concentrations of 0-3.0% (w/v). Phylogenetic and 16S rRNA gene nucleotide sequence analysis confirmed that strain WR6T affiliated to the genus Mesorhizobium, with the nearest relative being Mesorhizobium waimense ICMP 19557T (98.5%). The genome of strain WR6T was 5,035,462 bp with DNA G+C content of 62.6%. In strain WR6T, Q-10 was sole ubiquinone; summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c were predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were major polar lipids. Based on these polyphasic taxonomic data, strain WR6T represents a novel species in the genus Mesorhizobium. Accordingly, we propose the name Mesorhizobium koreense sp. nov., with the type strain WR6T (=KCTC 92695T =NBRC 116021T).","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11473501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Bacterial Therapies and Genetic Engineering Approaches in Colorectal Cancer: A Review of Emerging Strategies and Clinical Implications. 大肠癌的创新细菌疗法和基因工程方法：新策略和临床意义综述》。

IF 2.5 4区生物学

Journal of microbiology and biotechnology Pub Date : 2024-09-25 DOI: 10.4014/jmb.2408.08026

Chunxiao Song, Chunwu Zhao

{"title":"Innovative Bacterial Therapies and Genetic Engineering Approaches in Colorectal Cancer: A Review of Emerging Strategies and Clinical Implications.","authors":"Chunxiao Song, Chunwu Zhao","doi":"10.4014/jmb.2408.08026","DOIUrl":"https://doi.org/10.4014/jmb.2408.08026","url":null,"abstract":"Colorectal cancer (CRC) is considered a widespread cancer, ranking second in mortality and incidence among cancer patients worldwide. CRC develops from adenoma to carcinoma through the dynamic interplay of genetic and environmental factors. The conventional modes of treatment, including operation, chemotherapy, and irradiation, are associated with significant challenges, such as drug resistance and toxicity, necessitating the exploration of new treatment modalities. These difficulties reveal the necessity of the emergence of new therapeutic approaches. This review mainly emphasizes the bacterial-based therapies that have recently developed like the engineered bacteriophage therapy and bacterial immunotherapy that pale the existing chemotherapy in terms of toxicity but are effective in killing tumor cells. Also, it also investigates various molecular genetic engineering strategies such as CRISPR-Cas9, CRISPR prime editing and gene silencing to achieve better targeting of CRC. Implementing these new approaches into the forefront of CRC treatment may bring better, more effective therapy with fewer side effects on patients' quality of life.","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0