Journal of microbiology and biotechnology最新文献

{"title":"Toward Complex Polyketide Biosynthesis in <i>Yarrowia lipolytica</i> via Metabolic Reprogramming.","authors":"Kian Ghaempanah, So-Hee Son, Ju Young Lee","doi":"10.4014/jmb.2606.06035","DOIUrl":"10.4014/jmb.2606.06035","url":null,"abstract":"<p><p>Polyketides are among the most structurally diverse and therapeutically important classes of natural products, serving as antibiotics, anticancer agents, agrochemicals, and industrial pigments. Their structural complexity and limited natural availability have driven the development of microbial biosynthetic platforms as scalable and sustainable alternatives to traditional extraction or chemical synthesis. <i>Yarrowia lipolytica</i>, a non-conventional and metabolically versatile yeast, has emerged as a promising alternative chassis for polyketide biomanufacturing, owing to its streamlined central metabolism, high acetyl-CoA availability, and exceptional physiological robustness. Recent advances in metabolic engineering and synthetic biology have enabled extensive rewiring of <i>Y. lipolytica</i> metabolism to support the high-yield production of complex, high-value polyketides. This review summarizes state-of-the-art strategies, from classical metabolic rewiring to emerging approaches such as organelle engineering and subcellular compartmentalization. We further highlight representative case studies of polyketide biosynthesis in <i>Y. lipolytica</i>, critically assess current limitations, and explore future directions to establish this organism as a programmable, industrially viable platform for polyketide production.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2606035"},"PeriodicalIF":3.4,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of Fungi and Fungal-Derived Ingredients in the QPS and GRAS Systems.","authors":"He-Jin Cho, Yeon Ju Lee, Young Hoon Jung, Hee-Soo Park","doi":"10.4014/jmb.2607.07007","DOIUrl":"10.4014/jmb.2607.07007","url":null,"abstract":"<p><p>Filamentous fungi are representative eukaryotic microorganisms that are widely used in the food industry for the production of organic acids, enzymes, minerals, and other value-added compounds. However, ensuring their safety is a prerequisite for their application in food production. Two major regulatory approaches relevant to the safety of microbial food production are the U.S. Food and Drug Administration (FDA) Generally Recognized as Safe (GRAS) framework and the European Food Safety Authority (EFSA) Qualified Presumption of Safety (QPS) approach. The EFSA QPS framework evaluates eligible taxonomic units, whereas FDA GRAS Notices concern specified substances produced under defined conditions, including substances manufactured using yeasts, filamentous fungi, and mushrooms. Among these, several species of filamentous fungi, particularly members of the genus <i>Aspergillus</i>, have been widely used for the industrial production of food enzymes and other commercially important bioproducts. Notably, selected strains of <i>Aspergillus oryzae</i> and <i>Aspergillus niger</i> have a long history of industrial use in the production of enzymes, organic acids, and other industrial products. Enzymes produced by <i>A. oryzae</i> and <i>A. niger</i> are used in food processing as well as in animal feed to improve animal nutrition and digestion. This review provides a comprehensive overview of the <i>Aspergillus</i> taxa evaluated under the QPS approach and GRAS Notices involving <i>Aspergillus</i> production organisms or derived substances and discusses their current and potential applications in the food industry.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2607007"},"PeriodicalIF":3.4,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13558042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Bifidobacterium breve</i> B-3 on Glycemic Control and Glucose-Regulatory Responses in db/db Mice.","authors":"Sungmin Cho, Yewon Jeong, Jae-Hoon Kim, Yuri Gwon, Hakdong Shin, Wonchul Lim, Tae-Gyu Lim","doi":"10.4014/jmb.2605.05040","DOIUrl":"10.4014/jmb.2605.05040","url":null,"abstract":"<p><p>Type 2 diabetes mellitus is characterized by chronic hyperglycemia and impaired glucose homeostasis. This study evaluated the anti-diabetic effects of <i>Bifidobacterium breve</i> B-3 using <i>in vitro</i> models and db/db mice. <i>B. breve</i> B-3 inhibited α-amylase activity and enhanced insulin-stimulated glucose uptake in palmitic acid-induced insulin-resistant C2C12 myotubes. Metabolite profiling also showed distinct strain-associated metabolic characteristics of <i>B. breve</i> B-3. In db/db mice, oral administration of <i>B. breve</i> B-3 significantly reduced fasting blood glucose levels and improved oral glucose tolerance without affecting body weight. These effects were accompanied by changes in incretin-related endocrine responses, including increased serum GLP-1 levels and decreased DPP-4 activity. <i>B. breve</i> B-3 also increased serum insulin and C-peptide levels and partially preserved pancreatic insulin immunoreactivity. In addition, mild diabetes-associated intestinal morphological alterations and glucose-regulatory marker expression were partially normalized after administration. In skeletal muscle, <i>B. breve</i> B-3 was associated with increased expression of glucose uptake- and metabolic signaling-related markers, including AKT/GLUT4 and ADIPOR1/AMPK pathways. Overall, these findings suggest that <i>B. breve</i> B-3 improves glycemic control in db/db mice in association with changes in incretin-related endocrine responses and glucose-regulatory signaling markers.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2605040"},"PeriodicalIF":3.4,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13526894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Hyunsoonleella zeaxanthinifaciens</i> sp. nov., Isolated from Coastal Seawater of Jeju Island, Republic of Korea.","authors":"Ju-Hoon An, Jong Woo Hyeon, Mehwish Taj, Min-Hye Lee, Hanbi Kim, Sungjin Jung, Sang-Ah Lee, Man-Young Jung","doi":"10.4014/jmb.2606.06040","DOIUrl":"10.4014/jmb.2606.06040","url":null,"abstract":"<p><p>A Gram-stain-negative, strictly aerobic, yellow-pigmented, non-motile, rod-shaped rod-shaped bacterium, designated J2K-J805^T, was isolated from coastal seawater of Jeju Island, Republic of Korea. Strain J2K-J805^T grew at 15-37°C (optimum, 30°C), pH 6.0-8.0 (optimum, pH 7.0-7.5) and in the presence of 1-4% (w/v) NaCl (optimum, 2-3%) and was catalase-positive and oxidase-negative. The strain showed the highest 16S rRNA gene sequence similarity to <i>Hyunsoonleella flava</i> T58^T (98.4%). Phylogenetic and phylogenomic analyses based on the 16S rRNA gene and whole-genome sequences placed strain J2K-J805^T within the genus <i>Hyunsoonleella</i> as a distinct lineage. The OrthoANIu and digital DNA-DNA hybridization values between strain J2K-J805^T and <i>H. flava</i> T58^T were 87.7 and 34.5%, respectively, both below their respective thresholds for species delineation. The genome size and DNA G+C content were 3,825,400 bp and 34.9 mol%, respectively. The predominant respiratory quinone was MK-6, and the major cellular fatty acids were iso-C_15:1 G, iso-C_15:0 and iso-C_17:0 3-OH. The polar lipid profile comprised phosphatidylethanolamine, three unidentified aminolipids, two unidentified glycolipids and five unidentified lipids. High-performance liquid chromatography with photodiode-array detection identified the major yellow pigment as zeaxanthin, and the genome contained the carotenoid biosynthesis genes <i>crtB, crtI, crtY</i> and <i>crtZ</i> together with genes for isoprenoid precursor supply. On the basis of these polyphasic data, strain J2K-J805^T represents a novel species, for which the name <i>Hyunsoonleella zeaxanthinifaciens</i> sp. nov. is proposed. The type strain is J2K-J805^T (= KCTC 102498^T = JCM 38469^T).</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2606040"},"PeriodicalIF":3.4,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13506255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MPRO3 Regulates Macrophage Polarization and Osteoclastogenesis <i>In Vitro</i> and Attenuates Ligature-Induced Alveolar Bone Loss in Periodontitis.","authors":"Hyun-Joo Park, Tae Sung Kim, Mi-Kyoung Kim, Yeon Kim, Soon Chul Heo, Dong Ki Hong, Soo Dong Park, Jae Jung Shim, Jae Hwan Lee, Hyung Joon Kim, Soo-Kyung Bae, Moon-Kyoung Bae","doi":"10.4014/jmb.2604.04011","DOIUrl":"10.4014/jmb.2604.04011","url":null,"abstract":"<p><p>Periodontal disease is a chronic inflammatory disorder characterized by the destruction of periodontal tissues. It is caused by microbial biofilm deposition and altered host immune responses. Prebiotics, probiotics, and postbiotics have recently gained attention as complementary therapeutic strategies against periodontal disease. Here, we investigated whether MPRO3, a formulation containing heat-killed <i>Bifidobacterium animalis</i> subsp. <i>lactis</i>, <i>Lactiplantibacillus plantarum</i>, and <i>Lacticaseibacillus paracasei</i>, could mitigate periodontal inflammation and bone resorption. Treatment of THP-1 cells with MPRO3 attenuated <i>Porphyromonas gingivalis</i> lipopolysaccharide-induced M1 polarization, while enhancing M2 polarization instead. In addition, MPRO3 suppressed osteoclast differentiation of bone marrow-derived macrophages and regulated the expression of osteoclastogenesis-related factors. In a mouse model of ligature-induced periodontitis, MPRO3 significantly attenuated alveolar bone loss, accompanied by decreased TRAP-positive osteoclasts, reduced RANKL expression, and lower systemic pro-inflammatory cytokine levels. Collectively, these findings indicate that MPRO3 regulates macrophage polarization and osteoclastogenesis, highlighting its potential as an adjunctive intervention in periodontal disease management.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604011"},"PeriodicalIF":3.4,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13519251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148829371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory Effects of <i>Tenebrio molitor</i> Larva Trypsin Hydrolysate on Androgenetic Alopecia.","authors":"Seok-Hee Kim, Young-Jin Choi, Nishala Erandi Wedamulla, Qun Zhang, Jin-Hwan Lee, Eun-Kyung Kim","doi":"10.4014/jmb.2606.06002","DOIUrl":"10.4014/jmb.2606.06002","url":null,"abstract":"<p><p>Androgenetic alopecia (AGA) is the most common form of hair loss, with a high prevalence worldwide. In this study, we aimed to investigate the potential of <i>Tenebrio molitor</i> larva trypsin hydrolysate (TMTH), an insect-derived bioactive hydrolysate, as a functional ingredient candidate for AGA. <i>In vitro</i> and <i>in vivo</i> assessments demonstrated that TMTH promoted human hair dermal papilla (HDP) cell proliferation, restored β-catenin expression, and alleviated dihydrotestosterone (DHT)-induced inhibition. In a testosterone propionate (TP)-induced alopecia mouse model, oral TMTH administration increased hair coverage and follicle number, upregulated Wnt/β-catenin and PI3K/Akt signaling, reduced androgen receptor expression, enhanced antioxidant-related responses, and suppressed inflammatory markers. These findings suggest that TMTH may ameliorate AGA-related phenotypes by modulating hair growth-related signaling, local androgen-related responses, oxidative stress, and inflammation, thereby supporting its potential as a functional ingredient candidate for hair loss management.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2606002"},"PeriodicalIF":3.4,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13591393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Supportive Effects of AP Collagen Peptides on Skin Hydration in a Mouse Model of Oxazolone-Induced Atopic Dermatitis.","authors":"Su-Young Kim, A Yeon Park, Hyun Joo Lee, Eo Jin Kim, Ayeon Choi, Sun Young Choi, Beom Joon Kim","doi":"10.4014/jmb.2604.04022","DOIUrl":"10.4014/jmb.2604.04022","url":null,"abstract":"<p><p>Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disorder characterized by impaired skin barrier function and Th2-mediated immune responses. Collagen tripeptide (CTP), an amino acid-rich dietary supplement with low antigenicity, has been reported to improve skin hydration and barrier function. However, its therapeutic effects in AD, particularly under conditions of barrier dysfunction, have not yet been fully established. In this study, we evaluated the potential effects of AP collagen peptide (APCP), which contains CTP, on skin hydration and inflammatory responses, in an oxazolone (OXZ)-induced AD-like model using hairless mice. Skin hydration and transepidermal water loss (TEWL) were measured, while inflammatory responses were analyzed by histological evaluation and RT-qPCR. APCP-treated mice showed a tendency toward improvement in AD-like clinical symptoms and TEWL. In addition, APCP administration significantly increased skin hydration, accompanied by restoration of filaggrin expression and significantly reduced expression of TSLP, p-STAT3, and p-ERK. In contrast, mast cell infiltration, CD3⁺ and CD4⁺ T cell infiltration, and the expression of inflammatory cytokines, including IFN-γ, IL-4, IL-13, and IL-31, showed a tendency toward reduction but did not reach statistical significance. These findings suggest that APCP may not exert broad immunosuppressive effects, but may contribute to the alleviation of AD symptoms through improvements in skin hydration, supporting its potential as an adjunctive agent for AD.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604022"},"PeriodicalIF":3.4,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13526892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bifidobacterium breve</i> CBT BR3 and <i>Lactiplantibacillus plantarum</i> CBT LP3 Alleviate Hepatic Steatosis by Suppressing Hepatic Lipogenesis and Improving Metabolic Hormone Profiles <i>In Vitro</i> and <i>In Vivo</i>.","authors":"Yeongju Yeo, Jong Won Kim, Yusook Chung, Gyeyeong Kong, Misun Yun, Sanghyun Lim","doi":"10.4014/jmb.2604.04074","DOIUrl":"10.4014/jmb.2604.04074","url":null,"abstract":"<p><p>Hepatic steatosis is an early key event in metabolic dysfunction-associated steatotic liver disease, for which safe and effective interventions for reducing hepatic lipid accumulation are needed. To this end, we investigated the anti-steatotic effect of combining probiotics <i>Bifidobacterium breve</i> BR3 and <i>Lactiplantibacillus plantarum</i> LP3 (BR3LP3) on an <i>in vitro</i> oleic acid (OA)-treated human hepatocellular carcinoma (HepG2) cell model and an <i>in vivo</i> high-fat diet (HFD)-induced obese mouse model. In the OA-treated HepG2 cell model, BR3LP3 reduced OA-induced lipid accumulation without exhibiting cytotoxicity and modulated lipogenesis-related protein expression, while in the HFD-fed obese mouse model, oral BR3LP3 supplementation reduced body weight, fat mass, liver weight, and epididymal adipose tissue weight and improved several serum lipid, liver injury-related, and metabolic hormone parameters without affecting food intake. Moreover, when comparing BR3LP3-supplemented mice with those fed the HFD only, the former revealed reduced hepatic lipid droplet accumulation via histological analysis, while the expression levels of sterol regulatory element-binding protein-1c (<i>Srebp1c</i>), peroxisome proliferator-activated receptor γ (<i>Pparγ</i>), CCAAT/enhancer-binding protein alpha (<i>Cebpα</i>), acetyl-CoA carboxylase (<i>Acc</i>), and fatty acid synthase (<i>Fas</i>) were lower. Hence, our findings indicate that BR3LP3 supplementation attenuates hepatic lipid accumulation and the expression of hepatic lipogenesis-related markers while improving adiposity and systemic metabolic parameters. This suggests that BR3LP3 supplementation is a promising probiotic dietary strategy for improving hepatic steatosis and related metabolic dysfunction.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2604074"},"PeriodicalIF":3.4,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13519248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148829354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented Navy Bean-Derived Agents Restore Antibiotic Susceptibility in <i>Escherichia coli</i> Persisting on Fresh Produce after Washing through Transcriptional Modulation of Genes Associated with an <i>rpoS</i>-Centered Regulatory Network.","authors":"Sojeong Park, Se-Young Cho, Chyer Kim, Duwoon Kim","doi":"10.4014/jmb.2607.07019","DOIUrl":"10.4014/jmb.2607.07019","url":null,"abstract":"<p><p>Mechanical washing is an essential practice for reducing microbial contamination on fresh produce, yet the physiological adaptation of bacteria that persist after washing remains poorly understood. Here, we investigated the adaptive responses of lettuce-associated <i>Escherichia coli</i> ATCC25922 that remained attached after mechanical washing and evaluated the effects of fermented navy bean extract (FBE) and a PCC13-62-derived synthetic peptide (MFP-1). Saline washing detached only 25.1±3.9% of the attached bacterial population as the wash-removed population (WRP), while the majority of cells remained as a surface-retained population (SRP). Compared with the WRP, the SRP exhibited markedly higher survival following ampicillin treatment, accompanied by coordinated upregulation of stress-response genes (<i>rpoS</i>, <i>dnaK</i>, and <i>dps</i>), adhesion (<i>csgD</i>), and multidrug efflux (<i>marA</i>, <i>acrA</i>, <i>acrB</i>, and <i>tolC</i>) genes. In contrast, washing with FBE or MFP-1 at 200 μg/mL significantly increased recovery of the WRP to 80.8±5.4% without affecting bacterial viability, while suppressing expression of these stress-response, adhesion, and multidrug efflux genes in the ampicillin-resistant SRP (AMP^R-SRP), restoring ampicillin susceptibility, and thereby reducing AMP^R-SRP survival following ampicillin treatment to 2.4-2.8 log CFU/mL. These findings indicate that bacteria remaining attached after washing is associated with enhanced antibiotic resistance and concerted transcriptional activation of a proposed <i>rpoS</i>-centered regulatory network. FBE and MFP-1 effectively restored antibiotic susceptibility associated with transcriptional suppression of genes involved in this adaptive response, highlighting their potential as functional washing additives for improving the microbial safety of fresh produce.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2607019"},"PeriodicalIF":3.4,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compositional and Functional Characterization of Glycolipid Biosurfactants from an Olive-Derived <i>Lactiplantibacillus plantarum</i>.","authors":"Jong Woo Hyeon, Min Hui Jeon, Jun-Tae Bae, Man-Young Jung","doi":"10.4014/jmb.2606.06027","DOIUrl":"10.4014/jmb.2606.06027","url":null,"abstract":"<p><p>Biosurfactants from lactic acid bacteria are attractive as safe, multifunctional ingredients for food and cosmetic applications, yet many remain only partially characterized. An olive-derived strain was identified as <i>Lactiplantibacillus plantarum</i> J2K-1229 by 16S rRNA gene and genome-based phylogeny. Grown in rapeseed oil-containing medium, it secreted extracellular material with surface/interfacial activity, and its cell-free supernatant emulsified diverse plant oils (emulsification index after 24 h, 26.67-41.33%). A purified biosurfactant fraction gave carbohydrate- and lipid-positive, ninhydrin-negative thin-layer chromatography and Fourier transform infrared bands of a hydroxylated, ester- and carboxylic acid-containing lipid. Gas chromatography, NMR, and HPAEC-PAD indicated a complex, lipid-dominant mixture with major oleic and palmitic acids, NMR signals consistent with co-extracted phenyllactic acid-type metabolites, and fructose as the only detected monosaccharide. Genome mining revealed glucosyltransferases for glucose-based glycolipids but no fructosyltransferase, suggesting that the detected fructose may represent co-extracted free fructose, possibly released as a glucansucrase by-product. The preparation inhibited <i>Candida albicans</i> in disk diffusion assays and was non-cytotoxic to RAW 264.7 macrophages and HaCaT keratinocytes up to 200 ppm. In lipopolysaccharide-stimulated macrophages it suppressed nitric oxide production (88.07% at 200 ppm) and inducible nitric oxide synthase expression, and in cytokine-stimulated keratinocytes it reduced TARC, MDC, and RANTES expression. Overall, this olive-derived <i>L. plantarum</i> produces a multicomponent, glycolipid-containing biosurfactant preparation with emulsifying, antifungal, and anti-inflammatory activities suited to cosmetic and personal-care applications.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"36 ","pages":"e2606027"},"PeriodicalIF":3.4,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13456973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}