Journal of Medical Biochemistry最新文献

{"title":"Effects of sacral nerve magnetic stimulation on oxidative stress, inflammation and coagulation function in patients with neurogenic bladder after spinal cord injury.","authors":"Ruimin Wu, Jin Li","doi":"10.5937/jomb0-61262","DOIUrl":"10.5937/jomb0-61262","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the effect of sacral nerve magnetic stimulation (SNM) on the neurogenic bladder (NB) following spinal cord injury (SCI). Pay close attention to the changes in serum oxidative stress factors, inflammatory factors and coagulation function.</p><p><strong>Methods: </strong>A total of 134 SCI-induced NB patients admitted between February 2021 and January 2025 were enrolled. All participants received information-motivation-behavioural skills (IMB) management, and patients were grouped according to their treatment regimen: a conventional group (n=71) receiving conventional treatment and an SNM group (n = 63) treated with SNM (20 Hz frequency, 1 Tesla intensity, 20-minute sessions, once a day, 5 times a week, for 4 consecutive weeks). Laboratory analyses included coagulation markers (D-dimer, fibrinogen), oxidative stress indicators (superoxide dismutase [SOD], glutathione peroxidase [GSH-Px], and malondialdehyde [MDA]), and inflammatory mediators (IL-1<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">β</span>, IL-6, TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span>, and high-sensitivity C-reactive protein [hs-CRP]).</p><p><strong>Results: </strong>Coagulation function also improved, with lower D-dimer and fibrinogen levels in the SNM group (P&lt; 0.05). Regarding oxidative stress, the SNM group exhibited higher SOD and GSH-Px activity alongside reduced MDA levels (P&lt; 0.05). Furthermore, inflammatory markers (IL-6, TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span>) were lower in the SNM group (P&lt; 0.05).</p><p><strong>Conclusions: </strong>SNM can alleviate the stress response and inflammatory response of patients with SCI-induced NB, relieve the hypercoagulable state of blood, and has certain clinical application value.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"778-784"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk prognosis correlation of insulin-like growth factor binding protein-3 and insulin-like growth factor-1 in uterine fibroids.","authors":"Tingting Lin, Keke Qian, Yanping Chen, Mengshu Li, Zhang Zhang","doi":"10.5937/jomb0-60550","DOIUrl":"10.5937/jomb0-60550","url":null,"abstract":"<p><strong>Background: </strong>To explore the relationships between the levels of serum insulin-like growth factor binding protein-3 (IGFBP-3) and insulin-like growth factor 1 (IGF-1) and immune factors, as well as the prognosis of patients with uterine fibroids.</p><p><strong>Methods: </strong>The study group consisted of 186 patients with uterine fibroids who had laparoscopic myomectomy between June 2023 and June 2024, whereas the control group consisted of 208 healthy women who had physical tests over the same time period. The patients in the study group were divided into a good prognosis group (84 patients) and a poor prognosis group (92 patients). The levels of GFBP-3, IGF-1 and immune factors in the study group and the control group, and their relationships with prognosis were analysed.</p><p><strong>Results: </strong>Ten patients left the study group, and eight left the control group. IGFBP-3 and IGF-1 in the study group were (3 03.18± 42.39) mg/L and (1377.11 ± 84.78) mg/L, which both outperformed those in the control contingent [(231.2 5 ± 3 4 .1 8) mg/L and (4 3 8 .0 9 ± 5 2 .1 5) mg/L] (t= 12.87, 19.63) P&lt; 0.001). Whereas the levels of CD3+, CD 4+, and CD 4+/CD 8+ were lower than those in the control group (t= 7.92, 8.41, 5.21, P&lt; 0.001). Both values were higher [(284.63± 36.19) mg/L and (4 34.91± 53.28) mg/L]. P&lt;0.001 (t= 5.96, 64.19). While the numbers of CD 3+, CD 4+, and CD 4+/CD 8+ cells were lower than those in the good prognosis group (t= 5.31, 7.03, 3.15). There was a positive correlation between CD8+ status and IGFBP-3, as well as history of uterine fibroids, number of fibroids, history of miscarriage, and duration of breastfeeding (r= 0.593, 0.452, 0.446, 0.419, 0.422, respectively). IGF-1 was negatively correlated with CD 3+, CD 4+, and CD 4+/CD 8+ (r=-0.720, -0.751, and -0.712, respectively). IGF-1 was positively correlated with CD8+ status, history of uterine fibroids, number of fibroids, history of miscarriage, and duration of breastfeeding (r= 0.631, 0.503, 0.444, 0.501, 0.451). The number of fibroids, a history of miscarriage, and the length of breastfeeding were risk variables that impacted the prognosis of patients with uterine fibroids.</p><p><strong>Conclusions: </strong>The levels of IGFBP-3, IGF-1 and CD-8+ in patients with uterine fibroids are relatively high. Moreover, IGFBP-3 and IGF-1 are related to immune factors and patient prognosis.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"740-748"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the effect of platelet-derived growth factor combined with T cells in the diagnosis of hepatocellular carcinoma and prognostic assessment: A prospective cohort study.","authors":"Fei Wu, Guo Chen, Zuxin Wang, Youquan Niu, Wenqing Zhang, Hua Jiang","doi":"10.5937/jomb0-60968","DOIUrl":"https://doi.org/10.5937/jomb0-60968","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study is to investigate the diagnostic and prognostic value of combined detection of serum platelet-derived growth factor (PDGF) and Treg/Th17 in hepatocellular carcinoma (HCC).</p><p><strong>Methods: </strong>We included 204 HCC patients managed with drug eluting beads-transcatheter arterial chemoembolization (D-TACE) and 100 healthy controls. Serum PDGF concentrations were measured via enzyme-linked immunosorbent assay (ELISA), while flow cytometry quantified Treg and Th17 cell proportions. The predictive performance of PDGF, Th17/Treg, and their combination for HCC diagnosis, therapeutic response, and 1-year overall survival (OS) was evaluated using receiver operating characteristic (ROC) analysis, Kaplan-Meier survival curves.</p><p><strong>Results: </strong>HCC cases exhibited notable elevations in serum PDGF concentrations and Th17/Treg than healthy controls, with tumor tissue PDGF expression significantly correlating with serum levels (P &lt; 0.05). In addition, we found a positive correlation between PDGF, Th17/Treg and tumor markers in HCC patients (P &lt; 0.05). PDGF + Th17/Treg detection yielded an area under the curve (AUC) of 0.835 (68.14% sensitivity, 82.00% specificity), surpassing individual markers. For predicting D-TACE non-response (progressive disease, PD), the combined detection showed 71.70% sensitivity and 84.11% specificity. The 1-year OS rates of the high PDGF group and the high Th17/Treg group were 75.19% (97/129) and 61.22% (30/49), respectively, while the 1-year OS rates of the low PDGF group and the low Th17/Treg group were 92.00% (69/75) and 87.74% (136/155), respectively (P &lt; 0.05). The AUC of the combined model for predicting death was 0.705.</p><p><strong>Conclusions: </strong>PDGF combined with Th17/Treg showed excellent diagnostic value for the occurrence and poor prognosis of HCC.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"793-801"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel risk prediction model for post-treatment ischemic stroke recurrence using plasma antithrombin III and thromboelastography.","authors":"Yi Cheng, Baobao Huang, Shan Jiang","doi":"10.5937/jomb0-61916","DOIUrl":"https://doi.org/10.5937/jomb0-61916","url":null,"abstract":"<p><strong>Background: </strong>The primary aim of this investigation was to build and test a novel risk prediction model incorporating plasma antithrombin III (ATIII) activity and thrombelastography (TEG)-derived parameters. This approach seeks to enhance the ability to stratify the risk of stroke recurrence among ischemic stroke (IS) patients receiving dual antiplatelet therapy (DAPT).</p><p><strong>Methods: </strong>In this prospective cohort study, 200 consecutive patients diagnosed with non-cardiogenic IS were recruited during a one-year period (May 2024 to May 2025). All participants had their ATIII activity, TEG parameters, and coagulation function indicators tested within 24-72 hours of DAPT commencement. After initial variable screening via univariate analysis. A logistic regression-based risk model was built, with its ability to distinguish outcomes evaluated using the receiver operating characteristic (ROC) curve.</p><p><strong>Results: </strong>Recurrent events occurred in 21.00% (42/200) of cases within 3 months post-DAPT Multivariate analysis established ATIII, TEG-LY30, and D-Dimer as independent risk factors and TEG-MA as protective. The resultant model exhibited superior predictive power (A U C = 0.9480, 95% C I= 0.9l48~ 0.9813; sensitivity 90.48% , specificity 86.71%). Internal validation, yielding AUCs of 0.9521 in the training set and 0.9437 in the validation set, verified the model's strong generalizability. Subgroup evaluations further revealed the model's robust performance in both large artery atherosclerosis (AU C= 0.9505) and small vessel occlusion (AUC=0.9395) subtypes.</p><p><strong>Conclusions: </strong>Integrating ATIII activity with TEG parameters enables the development of a novel model to predict the risk of IS relapse following DAPT.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"723-730"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early diagnostic value of Serum GDF-15, RBP4 and SOCS3 in severe pneumonia with sepsis.","authors":"Mingying Luo, Ziyi Wang, Zhener Ke, Cong Fu","doi":"10.5937/jomb0-61152","DOIUrl":"https://doi.org/10.5937/jomb0-61152","url":null,"abstract":"<p><strong>Background: </strong>To explore the early diagnostic value of the serum levels of growth differentiation factor 15 (GDF-15), retinol binding protein 4 (RBP4), and cytokine signal transduction inhibitory factor 3 (SOCS3) in patients with sepsis caused by severe pneumonia (SP).</p><p><strong>Methods: </strong>A total of 110 SP patients were admitted to our hospital from December 2023 to February 2025. Based on whether they acquired sepsis, the patients were split into two groups: 52 patients with sepsis and 58 patients without. Additionally, 114 healthy individuals from the same age group who passed the health examination test at our hospital during the same period were selected as the control group. The levels of serum GDF-15, RBP4 and SOCS3 were detected via ELISA. Correlation analysis was conducted via the Pearson method. The factors influencing secondary sepsis in SP patients were examined using univariate and multivariate analysis. Serum GDF-15, RBP4, and SOCS3 levels were analysed for diagnostic effectiveness using a receiver operating characteristic (ROC) curve. The regional differences in the ROC curves of the serum samples were analysed via the paired comparison method.</p><p><strong>Results: </strong>RBP4 levels were substantially lower (P&lt; 0.05) while serum GDF-15 and SOCS3 levels were significantly higher in the study group. Compared with those in nonseptic patients, Serum GDF-15 and SOCS3 levels were considerably higher in SP patients in the sepsis group, whereas the level of RBP4 was significantly lower (P&lt; 0.05). The sepsis group's APACHE II score was substantially higher than the nonsepsis group's (P &lt; 0.05). The APACHE II score showed a favourable correlation (P&lt;0.05) with the levels of serum GDF-15 and SOCS3 in SP patients. The serum RBP4 level was negatively correlated with the APACHE II score (P&lt;0.05). Elevated levels of serum GDF-15 and SOCS3, along with increased APACHE II scores, are risk factors for sepsis in SP patients. In contrast, elevated serum RBP4 levels are a protective factor for sepsis in SP patients (P&lt;0.05). The area under the ROC curve (AUC) of the combined diagnosis of secondary sepsis in SP patients by serum GFF-15, RBP4 and SOCS3 levels was 0.946. The AUC of the combined diagnosis was superior to that of the individual diagnosis (Z= 1.970, 3.898, 3.188; P&lt;0.05).</p><p><strong>Conclusions: </strong>Serum GDF-15, RBP4, and SOCS3 levels in SP patients fluctuate in tandem with the severity of the patient's illness and subsequent sepsis. The combined diagnosis of these three factors has a certain value for secondary sepsis in SP patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"927-935"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of FOXO3 and Irisin in evaluating posterior cruciate ligament and knee functional recovery after surgical treatment of tibial avulsion fracture of the posterior cruciate ligament insertion.","authors":"Wenbin Shen, Yao Xiao, Xin Yu, You Zhang","doi":"10.5937/jomb0-60053","DOIUrl":"10.5937/jomb0-60053","url":null,"abstract":"<p><strong>Background: </strong>To explore the predictive value of serum forkhead box protein O3 (FOXO3) and Irisin in evaluating posterior cruciate ligament (PCL) and knee functional recovery after surgical treatment of tibial avulsion fractures (TAFs) of the PCL insertion (PCL-TAFs).</p><p><strong>Methods: </strong>In this prospective cohort study, we recruited 125 patients with PCL-TAFs (research group) and 111 healthy controls (control group) between October 2023 and December 2024. Serum FOXO3 and Irisin levels were detected at preoperative baseline and 6-month follow-up. Clinical evaluations included Lysholm scores, knee joint range of motion (ROM), posterior drawer testing, and International Knee Documentation Committee Subjective Knee Form (IKDC) scores. Pearson correlation and ROC analyses examined biomarker associations with functional recovery.</p><p><strong>Results: </strong>PCL-TAF patients exhibited higher preoperative FOXO3 levels than controls, which decreased following treatment (P &lt; 0.05). Conversely, Irisin levels were initially lower than controls but increased post-treatment (P &lt; 0.05). The predictive performance of combined FOXO3 and Irisin yielded 70.59% sensitivity and 94.51% specificity for detecting posterior drawer test positivity (AU C= 0.880). FOXO3 and Irisin together could identify severe dysfunction (IKDC III-IV) with 75.76% sensitivity and 77.17% specificity (AU C= 0.784).</p><p><strong>Conclusions: </strong>Serum FOXO3 and Irisin show clinical utility as potential biomarkers for functional recovery monitoring following PCL-TAF surgery, providing an objective molecular basis, as evidenced by ROC analysis, for the formulation of individualized rehabilitation plans.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"802-810"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triple detection of the tumor proteins p63, Ki-67 and Alpha-methylacyl-CoA racemase in the pathological diagnosis of prostate cancer.","authors":"Ming Niu, Danbo Zhao, Ruixue He, Ling Qin, Zhikai Li, Side Zhang, Lijun Chen","doi":"10.5937/jomb0-60143","DOIUrl":"10.5937/jomb0-60143","url":null,"abstract":"<p><strong>Background: </strong>To explore the value and significance of tripleimmunohistochemical detection of alpha-methylacyl-CoAracemase (AMACR), p63 and Ki-67 in the pathologicaldiagnosis of prostate cancer.</p><p><strong>Methods: </strong>A total of 156 paraffin-archived prostate biopsysamples collected from our hospital from June 2023 toAugust 2024 were selected as research materials. Theexpression levels of AMACR, p63 and Ki-67 in puncturesamples from 48 patients with prostate cancer, 32 patientswith high-grade prostate intraepithelial neoplasia, 26patients with low-grade prostate intraepithelial neoplasiaand 50 patients with benign prostatic hyperplasia weredetected via immunohistochemistry.</p><p><strong>Results: </strong>There were statistically significant differences in thepositive expression rates of AMACR, p63 and Ki-67 amongthe different groups of samples. The positive expression rateof AMACR in the puncture samples of the prostate cancergroup was 100%, with a high expression rate of 81.25%. The negative expression rate of p63 was 97.92%, whichwas significantly greater than that of the other three groups.The positive expression rate of Ki-67 was 81.25%, and thehigh expression rate was 54.17%. The AMACR is related tothe long diameter of the tumour, TNM stage, degree of differentiation and Gleason score in patients with prostate cancer. The rates of high AMACR in patients with tumours withlong diameters S1.5 cm, stage II-III, moderate to highly differentiated, and Gleason scores ranging from 8-10 weresignificantly higher than those in patients with long diameters &lt;1.5 cm, stage I. The expression of Ki-67 is related tothe long diameter of the tumour, degree of differentiation,degree of lymph node metastasis and Gleason score inpatients with prostate cancer. The rates of high expressionof Ki-67 in patients with a long tumor diameter of S1.5 cm,moderate and high differentiation, and lymph node metastasis and a Gleason score of 8-10 were significantly greaterthan those in patients with a long tumor diameter of &lt;1.5cm, poor differentiation, no lymph node metastasis, and aGleason score of 2-7. p63 was not related to the clinical orpathological characteristics of patients with prostate cancer(all P&gt;0.05). The sensitivity and negative predictive value ofthe combined diagnosis of AMACR-positive/p63-negative/Ki-67-positive prostate cancer were both 100.00%,and the specificity was 81.82%.</p><p><strong>Conclusions: </strong>AMACR, p63 and Ki-67 in prostate biopsysamples can be used as promising biomarkers for the diagnosis or exclusion of prostate cancer. Combined detectioncan improve the accuracy of prostate cancer diagnosis.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"811-818"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of serum PDK4, NLRP3, and PTEN levels in myocardial injury with sepsis.","authors":"Zebin Fang, Xiaoxia Li, Zhenfei Ou, Jimu Wang, Keji Lin, Shujia Ye","doi":"10.5937/jomb0-60967","DOIUrl":"10.5937/jomb0-60967","url":null,"abstract":"<p><strong>Background: </strong>To explore the correlations between the levels of serum pyruvate dehydrogenase kinase 4 (PDK4), NOD-like receptor protein 3 (NLRP3), and phosphatase and tensin homolog (PTEN) deleted on chromosome 10 and myocardial injury indicators, as well as prognosis in patients with sepsis.</p><p><strong>Methods: </strong>A total of 355 patients who were first diagnosed with sepsis and admitted to our hospital from September 2022 to September 2024 were included. The patients were divided into two groups based on their degree of myocardial damage: the septic myocardial injury (SIMI) group (225 patients) and the simple sepsis group (130 patients). The patients were divided into two groups according to their survival outcomes after 28 days of treatment: the death group (56 patients) and the survival group (169 patients). The levels of serum PDK4, NLRP3 and PTEN were detected via real-time fluorescence quantitative PCR (qRT PCR). An automatic biochemical analyser was used to measure the levels of myoglobin (Mb), cardiac troponin I (cTnI), creatine kinase isoenzyme (CK-MB), and heart-type fatty acid binding protein (H-FABP). The Pearson correlation coefficient method was used to analyse the relationships between serum PDK4, NLRP3, and PTEN levels and myocardial injury indicators in SIMI patients. Cox regression analysis was employed to examine the variables affecting SIMI patients' prognoses.</p><p><strong>Results: </strong>The levels of serum PDK4, NLRP3, PTEN, CK-MB, cTnI, Mb and H-FABP in the SIMI group were significantly greater than those in the simple sepsis group (P&lt;0.05). The levels of serum PDK4, NLRP3 and PTEN in patients with sepsis were positively correlated with the myocardial injury indicators CK-MB, cTnI, Mb and H-FABP (P&lt;0.05). The APACHE II score, SOFA score, cTnI, and the levels of serum PDK4, NLRP3 and PTEN in the prognosis death group of SIMI patients were greater than those in the survival group (P&lt;0.05). Independent risk variables that impact the prognosis of patients with SIMI include the APACHE II score, SOFA score, cTnI, PDK4, NLRP3, and PTEN (P&lt;0.05).</p><p><strong>Conclusions: </strong>Serum PDK4, NLRP3 and PTEN, which are independent risk factors affecting the prognosis of SIMI patients, are highly expressed in SIMI patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"865-873"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149038853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic monitoring of cognitive impairment and prognostic recurrence risk in patients with stroke by serum HDAC3 and FOXO1: A potential diagnostic approach.","authors":"Xintong Liu, Lulu Wang, Bin Xiao, Zhengkun Liu, Yue Dou","doi":"10.5937/jomb0-60965","DOIUrl":"10.5937/jomb0-60965","url":null,"abstract":"<p><strong>Background: </strong>To evaluate the clinical value of dynamically monitoring serum histone deacetylase 3 (HDAC3) and forkhead box protein O1 (FOXO1) levels in evaluating cognitive impairment (CI) in cerebral stroke (CS) patients, to enable early identification of high-risk individuals and guide targeted interventions.</p><p><strong>Methods: </strong>This study included 120 CS patients admitted from March, 2023 to March, 2024, with their serum HDAC3 and FOXO1 levels examined upon admission (T0) and at 2 (T1) and 24 hours (T2) following treatment. Differences in biomarker levels (CI group vs. non-CI group) were analyzed, and the predictive value of HDAC3 and FOXO1 for CI was determined. In addition, patients were followed up for 1 year prognosis, and the predictive effect of HDAC3 and FOXO1 on the prognostic recurrence risk of CS was analyzed.</p><p><strong>Results: </strong>CI occurred in 46 cases. HDAC3 expression was markedly increased in CI cases versus non-CI patients at T0, T1, and T2, whereas FOXO1 differed significantly only at T1 and T2 (P&lt; 0.05). HDAC3 and FOXO1 showed maximal predictive value for CI at the T2 timepoint. Similarly, the increase of HDAC3 and FOXO1 was also related to the risk of prognosis recurrence of CS patients. The sensitivity and specificity of combined detection of HDAC3 and FOXO1 in predicting the prognosis recurrence of CS patients were 84.38% and 78.41% (P&lt; 0.05).</p><p><strong>Conclusions: </strong>The pattern of dynamic HDAC3 and FOXO1 changes provides a new diagnostic scheme for the development of CI and prognostic recurrence risk in CS patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"767-777"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of FABP3, MCP-4, and CXCL9 levels and myocardial damage in patients with severe pneumonia.","authors":"Longxia Du, Jianping Wang, Zongxian Wu, Xianxin Lai, Xuanchen Qian","doi":"10.5937/jomb0-61605","DOIUrl":"https://doi.org/10.5937/jomb0-61605","url":null,"abstract":"<p><strong>Background: </strong>To explore the correlations between the levelsof serum monocyte chemoattractant protein-4 (MCP-4),heart-type fatty acid binding protein (FABP3), andchemokine ligand 9 (CXCL9) and myocardial damage insevere mycoplasma pneumonia (SMPP) patients.</p><p><strong>Methods: </strong>A total of 158 patients with severe mycoplasmapneumonia complicated with myocardial damage wereincluded in the SMPP group. They were divided into amyocardial damage group (n=42) and a nonmyocardialdamage group (n = 116) according to whether myocardialdamage occurred. The control group consisted of an additional 102 healthy people who were examined throughoutthe same time period. The levels of serum MCP-4, FABP3and CXCL9 in the two groups were compared. The patients'general clinical data were recorded. Multivariate logisticregression was used to identify risk factors for myocardialinjury in patients with severe mycoplasma pneumonia.</p><p><strong>Results: </strong>The levels of serum MCP-4, FABP3 and CXCL9 inthe SMPP group were significantly greater (all P&lt;0.05).Compared with those in the nonmyocardial damage group,serum MCP-4, FABP3, and CXCL9 levels were considerablyhigher (all P&lt; 0.05) in the group with myocardial injury Age, sex, diabetes, smoking history, hypoxemia, jaundice,and chronic obstructive pulmonary disease (COPD) did notdiffer statistically significantly between the two groups (allP&gt; 0.05). Compared with those in the nonmyocardial damage group, the proportions of patients with hypertension,coronary heart disease, and anaemia, as well as the levelsof serum MCP-4, FABP3, and CXC L9, in the myocardialdamage group were significantly higher (all P&lt;0.05).Combined hypertension, coronary heart disease, anaemia,and high levels of serum MCP-4, FABP3, and CXCL9 arerisk factors for myocardial damage in patients with severemycoplasma infection. The levels of serum MCP-4, FABP3and CXCL9 in patients were positively correlated with theincidence of myocardial damage in patients with severemycoplasma infection (all P&lt; 0.05).</p><p><strong>Conclusions: </strong>The levels of serum MCP-4, FABP3 and CXCL9are positively correlated with myocardial damage in patientswith severe mycoplasma pneumonia. Moreover, combinedhypertension, coronary heart disease, anaemia and high levels of serum MCP-4, FABP3 and CXCL9 are risk factors formyocardial damage in patients with severe mycoplasma infection. These three factors can serve as biological indicatorsof myocardial damage in patients with severe mycoplasmainfection in clinical practice and are highly important forassessing patients' conditions and formulating treatmentplans.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"936-944"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}