Journal of Medical Biochemistry最新文献

{"title":"Diagnostic value of immune indicators and cytokines in sepsis after percutaneous nephrolithotomy (PCNL).","authors":"Yiheng Jin, Yamei Li, Xiaoyu Zhang, Bing Han, Xingshi Yan","doi":"10.5937/jomb0-60616","DOIUrl":"10.5937/jomb0-60616","url":null,"abstract":"<p><strong>Background: </strong>To analyse the changes in and diagnostic value of immune indicators and cytokines in patients with sepsis after percutaneous nephrolithotomy (PCNL).</p><p><strong>Methods: </strong>Clinical information was gathered from 405 patients with calculi who underwent PCNL at our facility between January 2021 and December 2024. The patients were divided into a sepsis group (12 patients) and a non-sepsis group (393 patients) based on whether sepsis occurred after the operation. The levels of CD4+/CD 8 + cells, the neutrophil-to-lymphocyte ratio (NLR), soluble trigger receptor-1 (sTREM-1) in myeloid cells, procalcitonin (PCT), tumour necrosis factor-alpha (TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span>), and interleukin-6 (IL-6) were measured in the two patient groups. The predictive value of the above indicators for sepsis was analysed via receiver operating characteristic (ROC) curves.</p><p><strong>Results: </strong>The number of patients with staghorn calculi in the sepsis group was greater than that in the nonsepsis group (P&lt; 0.05). The NLR and sTREM-1 levels were greater in the sepsis group than in the nonsepsis group (all P&lt; 0.05), and the CD 4+/CD 8+ ratio was lower in the sepsis group than in the nonsepsis group (P&lt; 0.05). The levels of serum IL-6, TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> and PCT in the sepsis group were greater than those in the nonsepsis group (all P&lt; 0.05). The area under the curve (AUC) for the combined prediction of sepsis after PCNL by each index was 0.996, with a predictive sensitivity of 91.3% and a specificity of 98.4%.</p><p><strong>Conclusions: </strong>The NLR, sTREM-1, IL-6, TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> and PCT in patients with sepsis after PCNL increased, whereas the CD 4+/CD 8+ ratio decreased. The combined detection of these levels is beneficial for guiding the early clinical prediction of postoperative sepsis in patients undergoing PCNL.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"819-824"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum MIC-1, LMTK-3 and IGFBP-7 levels in the prognosis of lung cancer treated with ablation combined with chemotherapy.","authors":"Yilin Fu, Lifeng Shi, Quanxin Lu, Fatao Yu, Hong Xiaofeng, Zeng Guoliang, Meng Li","doi":"10.5937/jomb0-62184","DOIUrl":"10.5937/jomb0-62184","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To explore the value of the serum levels of macrophage inhibitory factor 1 (MIC-1), lemur tyrosine kinase 3 (LMTK-3), and insulin-like growth factor binding protein 7 (IGFBP-7) in the prognosis assessment of patients with advanced lung cancer treated with ablation combined with chemotherapy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;190 patients with advanced lung cancer who received care at this hospital between January 2022 and June 2024 were chosen to be part of the lung cancer group. The healthy control group consisted of 90 healthy individuals who attended the hospital and underwent physical tests during the same time period. Percutaneous microwave thermal ablation treatment was used to treat every patient with advanced lung cancer, and chemotherapy was administered within one week after treatment. The levels of serum MIC-1, LMTK-3, and IGFBP-7 before and after treatment, and before treatment, were compared between patients with different therapeutic effects in the lung cancer group and the healthy control group. Univariate and multivariate analyses were conducted to examine the factors affecting patient prognosis and the efficacy of serum MIC-1, LMTK-3, and IGFBP-7 levels in predicting patient prognosis in patients with advanced lung cancer.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Serum MIC-1 and LMTK-3 levels in the lung cancer group were substantially higher than those in the healthy control group both before and after therapy (P&lt;0,05). Serum MIC-1 and LMTK-3 levels in the lung cancer group were significantly lower after therapy than before (P&lt;0,05). The serum IGFBP-7 levels in the lung cancer group before and after treatment were significantly lower than those in the healthy control group (P&lt;0,05). After treatment, the serum IGFBP-7 level in the lung cancer group was significantly higher than before treatment (P&lt;0,05). Patients with lung cancer experienced 29 cases of stable response (SD)+ progressive response (PD) and 66 cases of complete response (CR)+ partial response (PR) following treatment. Those with SD + PD advanced lung cancer had significantly higher blood MIC-1 and LMTK-3 levels before treatment than those with CR+PR advanced lung cancer. The serum IGFBP-7 level of advanced lung cancer patients with SD+PD before treatment was significantly lower than that of advanced lung cancer patients with CR+PR. In patients with advanced lung cancer, elevated blood MIC-1 and LMTK-3 levels and lower IGFBP-7 levels before treatment were independent risk factors for death within a year following treatment (P&lt;0,05). The combined detection of serum MIC-1, LMTK-3, and IGFBP-7 levels for predicting death within 1 year after treatment for advanced lung cancer had a sensitivity of 91.3%, a specificity of 95.8%, and an AUC of 0.974. Its AUC was significantly greater than that of MIC-1 (Z=2.378, P=0.017) and LMTK-3 (Z=2.897). The AUC was separately assessed for IGFBP-7 (Z=3.213, P=0.001). However, the AUCs for the three i","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"856-864"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IRF4/FGF23 in gingival crevicular fluid: Diagnostic biomarkers for Dentin Hypersensitivity.","authors":"Song Can, Jia Yubao, Liu Yaxuan, Zhang Yujie, Yu Jing, Xian Mengmeng","doi":"10.5937/jomb0-60969","DOIUrl":"10.5937/jomb0-60969","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study is to investigate the diagnostic value of interferon regulatory factor 4 (IRF4) and fibroblast growth factor 23 (FGF23) in gingival crevicular fluid (GCF) and serum for dentin hypersensitivity (DH), and to analyze the relationship between IRF4, FGF23 and inflammatory factors and T lymphocyte subsets.</p><p><strong>Methods: </strong>24 DH patients receiving orthodontic treatment at our institution between 2022 and early 2025 were enrolled as the study cohort, along with 124 healthy controls matched for age and sex. GCF and serum samples were obtained 48 hours post-desensitization therapy to quantify IRF4 and FGF23 levels (via enzyme-linked immunosorbent assay), along with serum inflammatory markers (interleukin [IL]-1β, IL-6, high-sensitivity C-reactive protein [hs-CRP]) and T-cell subpopulations (CD3+, CD4+, CD8+). Statistical analyses included Pearson's correlation to examine relationships between IRF4/FGF23 and clinical parameters, with receiver operating characteristic (ROC) curve analysis determining diagnostic accuracy.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"671-679"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Values of D-dimer, APTT, PT and INR in distinguishing between acute ischemic stroke patients with and without non-valvular atrial fibrillation.","authors":"Qing Du, Xiaohui Zhao, Haixia Song, Ju Jiang, Kaiming Zheng, Yuezhan Zhang","doi":"10.5937/jomb0-60388","DOIUrl":"10.5937/jomb0-60388","url":null,"abstract":"<p><strong>Background: </strong>Treatment strategies differ in acute ischemic stroke (AIS) patients with and without non-valvular atrial fibrillation (NVAF): AIS with NVAF requires anticoagulation (e.g. warfarin/direct oral anticoagulants) for secondary prevention, while AIS without NVAF relies on antiplatelet therapy (e.g. aspirin/clopidogrel). It remains unclear whether coagulation function indices can be used to distinguish between the two conditions.</p><p><strong>Methods: </strong>In this retrospective study, AIS patients with NVAF (AIS with NVAF group, n=43) and without NVAF (AIS without NVAF group, n = 90) admitted to our hospital were enrolled for final analysis. The levels of coagulation function indices, including d-dimer (DD), activated partial thromboplastin time (APTT), prothrombin time (PT), international normalised ratio (INR), thrombin time (TT), and fibrinogen, were tested before or on admission and compared between the two groups.</p><p><strong>Results: </strong>Compared with AIS without NVAF group, DD, PT, and INR were increased and APTT was decreased in AIS with NVAF group (P&lt; 0.05), whereas, there were no significantly statistical differences of TT and fibrinogen (P&gt; 0.05); next, the AUC in distinguishing between AIS with and without NVAF were 0.753, 0.761, 0.775, and 0.770 for DD, APTT, PT, and INR, respectively (P&lt; 0.05).</p><p><strong>Conclusions: </strong>DD, APTT, PT and INR demonstrated moderate diagnostic value (AUC 0.75-0.78) for distinguishing AIS with NVAF.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"594-600"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of serum glycosylphosphatidylinositol mannosyltransferase 1 (GMP1) levels in type 2 diabetes mellitus patients.","authors":"Xiaolin Yang, Wang Qichang, Wei Chen, Jian Zhao","doi":"10.5937/jomb0-60118","DOIUrl":"10.5937/jomb0-60118","url":null,"abstract":"<p><strong>Background: </strong>To investigate the serum expression of glycosylphosphatidylinositol mannosyltransferase 1 (GMP1) in type 2 diabetes mellitus (T2DM) patients and its correlation with hypertriglyceridemia (HTG) to shed light on lipid metabolism disorders in T2DM patients.</p><p><strong>Methods: </strong>A total of 239 patients were included, among whom 92 patients were in the T2DM combined with HTG group and 147 patients were in the T2DM without HTG group. The concentration of the serum GMP1 protein was quantitatively detected via enzyme-linked immunosorbent assay (ELISA). Moreover, the levels of serum triglycerides (TGs) and other related metabolic indicators (such as blood glucose, glycated haemoglobin (HbA1c), total cholesterol, and high/low-density lipoprotein cholesterol) were detected via conventional biochemical methods. To evaluate the potential impact of GMP1 on the occurrence of T2DM combined with HTG.</p><p><strong>Results: </strong>Both the DM group and the DM + HTG group presented significantly higher serum GMP1 levels (P&lt; 0.01), and the GMP1 level in the DM +HTG group was considerably greater (P&lt; 0.05 or 0.01) than that in the simple DM group. The serum GMP1 levels were significantly greater in T2DM patients than in HTG patients. Serum GMP1 levels (O R = 1.527, 95% CI 1.200-1.943) were determined via binary logistic regression analysis. 95% CI 1.003-1.010) was a separate risk factor for HTG and T2DM. Correlation analysis revealed similar results in patients with T2DM (especially those in the DM +HTG group). Multiple regression analysis further indicated that after controlling for factors such as age, sex, disease duration, BMI, and HbA1c, higher blood GMP1 levels continued to be a predictor or independent factor for patients with T2DM complicated by HTG (P&lt; 0.05).</p><p><strong>Conclusions: </strong>Serum GMP1 levels are markedly elevated in T2DM patients, especially those with hypertriglyceridemia, and they are independently positively linked with triglycerides.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"550-557"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical evaluation of Jihua hemostatic suppository in hemorrhagic internal hemorrhoids: Modulation of XPO1, VEGF, and collagen I/III expression and inflammatory biomarkers.","authors":"Chao Wang, Yajun Kang, Ye Wang, Yujie Liu, Mengli Shi, Xiaoming Liu, Hao Jiao","doi":"10.5937/jomb0-62625","DOIUrl":"10.5937/jomb0-62625","url":null,"abstract":"<p><strong>Background: </strong>Hemorrhagic internal hemorrhoids involve abnormal vascular remodeling, inflammatory activation, and extracellular matrix dysregulation. Exportin-1 (Xpo1), vascular endothelial growth factor (VEGF), and collagen types I and III are key biochemical markers associated with these processes. This study aimed to evaluate the therapeutic efficacy of Jihua Hemostatic Suppository compared with Gangtai Suppository and to investigate their differential effects on biochemical and inflammatory parameters in patients with hemorrhagic internal hemorrhoids.</p><p><strong>Methods: </strong>A total of 98 patients diagnosed with hemorrhagic internal hemorrhoids were enrolled between January 2024 and January 2025 and randomly divided into two groups (n = 49 each) using the envelope randomization method. The Jihua group received Jihua Hemostatic Suppository, whereas the control group received Gangtai Suppository for two weeks. Serum levels of interleukin-6 (IL-6), tumor necrosis factor-a (TNF-a), and C-reactive protein (CRP) were determined by ELISA. Xpo1 levels were measured by ELISA, VEGF by chemiluminescence assay, and collagen types I and III by the hydroxyproline method. Clinical efficacy, traditional Chinese medicine (TCM) syndrome scores, and adverse reactions were also recorded.</p><p><strong>Results: </strong>The total effective rate in the Jihua group (93.88% ) was significantly higher than that in the Gangtai group (77.55% ) (c2 = 5.968, P = 0.015). After treatment, the Jihua group showed markedly lower serum IL-6 (70.50 ± 5.82 ng/mL), CRP (23.06 ± 2.71 mg/L), and TNF-a (60.81 ± 5.72 ng/mL) levels compared with the Gangtai group (P &lt; 0.001). Biochemically, Xpo1, VEGF, collagen I, and collagen III concentrations were significantly reduced in the Jihua group (12.31 ± 1.35 ng/mL, 389.64 ± 34.32 pg/mL, 154.39 ± 15.19 pg/L, and 36.13 ± 3.06 mg/L, respectively) compared with the Gangtai group (P &lt; 0.05). The incidence of adverse reactions was lower with Jihua suppository (4.08%) than with Gangtai suppository (20.41% ) (c2 = 4.242, P = 0.039).</p><p><strong>Conclusions: </strong>Jihua Hemostatic Suppository exerts superior therapeutic and biochemical effects in hemorrhagic internal hemorrhoids compared with Gangtai Suppository. Its efficacy is likely mediated by suppression of the Xpo1/NF-kB inflammatory axis, inhibition of VEGF-driven angiogenesis, and normalization of collagen I/III-dependent extracellular matrix remodeling. These findings highlight the value of integrating biochemical biomarkers into clinical assessment of hemorrhoidal disease and suggest Xpo1, VEGF, and collagen I/III as potential molecular indicators of treatment efficacy.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"636-644"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical effects of personalized clinical interventions on hepatorenal function, immune response, and inflammatory markers in patients with infections.","authors":"Ying Li, Ying Zhou, Jie Zhang, Yi Li, Xin Liu","doi":"10.5937/jomb0-62921","DOIUrl":"10.5937/jomb0-62921","url":null,"abstract":"<p><strong>Background: </strong>Hepatic and renal dysfunction, immune imbalance, and excessive inflammatory activation are common complications in postoperative infections. This study evaluated the biochemical effects of personalized clinical interventions on hepatorenal function, immune responses, and inflammatory cytokines in infected patients.</p><p><strong>Methods: </strong>A total of 110 patients with postoperative infections admitted between January 2023 and January 2025 were randomly assigned to an experimental group or a control group (n = 55 each). The control group received standard clinical care, while the experimental group underwent additional individualized intervention measures. Serum hepatic (AST, ALT) and renal (BUN, SCr, UA) biochemical markers, T-lymphocyte subsets (CD3+, CD4+, CD8+), inflammatory factors (IL-6, CRP TNF-a), and SF-36 quality-of-life scores were assessed before and after intervention. Adverse events were recorded.</p><p><strong>Results: </strong>Post-intervention, AST and ALT levels were significantly lower in the experimental group compared with the control group (P &lt; 0.05). BUN, SCr, and UA were also significantly reduced after individualized intervention (P &lt; 0.05). The experimental group demonstrated higher CD3+ and CD4+ levels and lower CD8+ levels than the control group (P &lt; 0.05). Levels of IL-6, CRP and TNF-a were markedly decreased in the experimental group (P &lt; 0.05). Quality-of-life scores across all domains were significantly higher following individualized intervention (P &lt; 0.05). The incidence of adverse reactions was notably lower in the experimental group (5.45% vs. 20.00%; c2 = 4.010, P = 0.045).</p><p><strong>Conclusions: </strong>Personalized clinical interventions significantly improved hepatic and renal biochemical indices, enhanced immune function, and reduced systemic inflammatory cytokines in patients with postoperative infections. These findings support the integration of individualized strategies into infection management to optimize biochemical and clinical outcomes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"655-662"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of elevated basal 17-hydroxyprogesterone in the diagnosis of children with congenital adrenal hyperplasia.","authors":"Jelena Miolski, Maja Ješić, Anita Skakić, Sonja Pavlović, Ivana Vorgučin, Vladislav Bojić, Smiljka Kovačević, Jelena Blagojević, Nevena Didić, Mirjana Doknić, Vera Zdravković","doi":"10.5937/jomb0-54426","DOIUrl":"10.5937/jomb0-54426","url":null,"abstract":"<p><strong>Background: </strong>Congenital adrenal hyperplasia (CAH) is usually characterised by a deficiency of 21a-hydroxylase, which causes a deficiency of cortisol and aldosterone and an overproduction of 17-hydroxyprogesterone. The classic and non-classical forms of the disease present ambiguous genitalia with signs of precocious puberty (PP) with accelerated height velocity and bone age. accelerated body and bone growth. Elevated basal and stimulated 17-hydroxyprogesterone and genetic testing are crucial for confirming a definitive diagnosis. The aim to determine determine the significance of elevated basal 17-hydroxyprogesterone in children with signs of precocious puberty in the final diagnosis of Congenital Adrenal Hyperplasia.</p><p><strong>Methods: </strong>A prospective study was conducted at the University Children's Clinic and the Institute of Molecular Genetics and Genetic Engineering in Belgrade from 2019 to 2024. The study involved 64 subjects of both sexes, aged up to 18 years, with precocious puberty, accelerated height velocity and bone age and/or elevated basal 17-hydroxyprogesterone, who were divided into two groups based on the presence/absence of pathogenic variants in the CYP21A2 gene. The anthropometric measures, skeletal maturation and hormone levels were compared between those two groups.</p><p><strong>Results: </strong>The research included 64 subjects, of whom 30 confirmed CAH and 34 were part of the control group with PR A statistically significant difference was shown in basal (p &lt; 0 .0 0 1 ) and stimulated 17-hydroxyprogesterone (p = 0 .0 1 3 ), cortisol (p = 0.015) and androstenedione (p= 0.014) in homozygous carriers of pathogenic variants in the CYP21A2 gene.</p><p><strong>Conclusions: </strong>Clinical and laboratory parameters such as precocious puberty and 17-hydroxyprogesterone may be significant hints to consider a carrier mutation for congenital adrenal hyperplasia.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"566-571"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of the latest CLSI EP guidelines in light-initiated chemiluminescent assay for testosterone.","authors":"Ai Liang, Cao Xiao-Jing, Ren Xin-Xin, Kang Lan, Zhang Dan, Xin Chang","doi":"10.5937/jomb0-58894","DOIUrl":"10.5937/jomb0-58894","url":null,"abstract":"<p><strong>Background: </strong>A commercial light-initiated chemiluminescent assay (LiCA) for testosterone (T) measurement was developed. This study comprehensively evaluated the analytical performance of LiCA-T following the Clinical and Laboratory Standards Institute (CLSI) Evaluation Protocols (EP) series guidelines.</p><p><strong>Methods: </strong>From July 1st 2023, both the manufacturer and clinical end-users conducted rigorous performance evaluations in accordance with CLSI EP guidelines. Assessment included precision, linearity, accuracy/trueness, detection limits, and interference testing using collected clinical samples. All the tests were carried out using LiCA® 800 (Chemclin, Beijing, China), and the reagent of lot 2202.</p><p><strong>Results: </strong>Manufacturer evaluations demonstrated excellent assay performance: repeatability (1.41-4.39%), intermediate imprecision (1.62-7.29%), and reproducibility (1.62-7.22%). The method showed linearity across 0.10-16.64 ng/mL (ADL=10%). Method comparison with MS reference yielded a limit of agreement (LoA) of -19.5% to 14.4%, with ≤10.00% bias at medical decision points. Sensitivity parameters included: LoB (0.041 ng/mL), LoD (0.049 ng/mL), and LoQ (0.060 ng/mL at 20% imprecision). The assay demonstrated robust interference resistance against haemoglobin (2 mg/mL), biotin (20 ng/mL), triglyceride (3 mg/mL), and bilirubin (0.157 mg/mL). All manufacturer claims were successfully verified by independent clinical end-user testing.</p><p><strong>Conclusions: </strong>The LiCA-T assay satisfies all performance criteria specified in CLSI latest EP guidelines (including EP06-Ed2 and EP07-Ed3), demonstrating reliable analytical performance across all critical validation parameters for clinical testosterone measurement.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"680-689"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid hormone sensitivity indices and hs-CRP as biochemical predictors of arteriovenous fistula maturation in diabetic nephropathy.","authors":"Dan Li, Bing Zhao, Lihong Zou","doi":"10.5937/jomb0-61296","DOIUrl":"10.5937/jomb0-61296","url":null,"abstract":"<p><strong>Background: </strong>Autogenous arteriovenous fistula (AVF) maturation is essential for successful hemodialysis in patients with diabetic nephropathy (DN). However, its outcome is often unpredictable. Thyroid hormone sensitivity indices and biochemical markers may provide valuable laboratory predictors for AVF maturation.</p><p><strong>Methods: </strong>A total of 102 DN patients undergoing AVF surgery were retrospectively analyzed and classified into mature and immature AVF groups. Laboratory data, including thyroid hormone sensitivity indices [free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), FT3/FT4 ratio, thyroid-stimulating hormone index (TSHI), thyroxine resistance index (TT4RI), thyroid feedback quantile index (TFQI), and parameterized TFQI (PFTQI)], as well as biochemical markers such as high-sensitivity C-reactive protein (hs-CRP), albumin, total cholesterol, triglycerides, and low-density lipoprotein cholesterol, were compared. Doppler ultrasonography was used as supportive assessment. Logistic regression identified independent predictors of AVF maturation.</p><p><strong>Results: </strong>Patients in the AVF Maturity Group exhibited significantly lower FT3, TSH, FT3/FT4 ratio, TSHI, TFQI, and PFTQI levels, along with reduced hs-CRP values (all P &lt; 0.05). Multivariate logistic regression revealed that higher FT4, TFQI, and PFTQI, lower hs-CRP, larger cephalic vein diameter, and younger age were independently associated with successful AVF maturation.</p><p><strong>Conclusions: </strong>Thyroid hormone sensitivity indices and hs-CRP serve as important biochemical predictors of AVF maturation in DN patients. These laboratory parameters may assist in risk stratification and clinical decision-making, providing a biochemical perspective for optimizing dialysis access outcomes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"520-528"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}