Journal of Medical Biochemistry最新文献

{"title":"Irrigation fluid temperature modulates coagulation and endothelial biomarkers in patients undergoing TURP.","authors":"Min Gu, Yanqiu Xu","doi":"10.5937/jomb0-61030","DOIUrl":"https://doi.org/10.5937/jomb0-61030","url":null,"abstract":"<p><strong>Background: </strong>The temperature of irrigation fluid is a crucial yet frequently neglected factor affecting perioperative hemostasis during transurethral resection of the prostate (TURP). Although the clinical implications of hypothermia are well-documented, its biochemical effects on coagulation processes and endothelial markers are not fully elucidated.</p><p><strong>Methods: </strong>Ninety patients with benign prostatic hyperplasia undergoing TURP were randomized into three groups based on irrigation fluid temperature: hypothermia (24-26 °C), mild hypothermia (28-35 °C), and preheating (36-37 °C). Peripheral blood was collected preoperatively and 6 h postoperatively to evaluate coagulation and endothelial indices, including prothrombin time (PT), activated partial thromboplastin time (APTT), platelet count (PLT), platelet aggregation rate (Pagt), and endothelin-1 (ET-1).</p><p><strong>Results: </strong>Hypothermia significantly prolonged APTT and reduced PLT and Pagt compared with preheating (P &lt; 0.05), indicating impaired intrinsic pathway activity and platelet dysfunction. PT remained unchanged across groups. ET-1 levels decreased in both hypothermia and preheating groups, with the greatest reduction in preheating, suggesting differential endothelial responses. Patients in the preheating group exhibited preserved coagulation stability and fewer adverse events (6.7%) compared with hypothermia (33.3%) and mild hypothermia (23.3%) groups (P = 0.038).</p><p><strong>Conclusions: </strong>Hypothermic irrigation significantly prolonged APTT, reduced platelet count, and impaired aggregation capacity, while preheating maintained hemostatic homeostasis.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"47-53"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of diagnostic and prognostic biomarker models for knee osteoarthritis based on NLRP3 inflammasome activation.","authors":"Shui Xiong, Junxin Zhou, Yuying Dong, Ling Long, Gaorong Deng","doi":"10.5937/jomb0-60351","DOIUrl":"https://doi.org/10.5937/jomb0-60351","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to characterize the expression profiles of the NOD-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome and its downstream effectors [Interleukin (IL)-1b, IL-18, and Gasdermin-D (GSDMD)] in degenerative knee osteoarthritis (KOA) and to establish an integrated biomarker model for predicting the likelihood of unfavorable rehabilitation outcomes.</p><p><strong>Methods: </strong>We conducted a retrospective study involving 121 KOA patients and 94 age-matched healthy controls. Serum concentrations of NLRP3, IL-1 b, and IL-18 were quantified using ELISA, while GSDMD expression in peripheral blood mononuclear cells was assessed through flow cytometry. Conventional inflammatory markers (CRP ESR, and WBC) and neutrophil-to-lymphocyte ratio (NLR) were measured using automated analyzers. Receiver operating characteristic (ROC) curve analysis and multivariate logistic regression were performed to evaluate the diagnostic and prognostic utility of the integrated biomarker model.</p><p><strong>Results: </strong>KOA patients exhibited significantly elevated levels of NLRP3, IL-1 b, IL-18, and GSDMD compared to healthy controls (P &lt; 0.05). These biomarkers showed positive correlations with systemic inflammatory markers (CRP ESR) and negative associations with knee joint range of motion (ROM) (P &lt; 0.05). The integrated biomarker model demonstrated robust diagnostic performance for KOA (AUC = 0.928, sensitivity 84.30% , specificity 87.23%). Notably, among treated patients, those with poor recovery (n=37) maintained significantly higher post-treatment levels of NLRP3 pathway components than those with favorable recovery (P &lt; 0.05). The predictive model achieved excellent performance in identifying patients at risk of suboptimal rehabilitation (AUC = 0.911, sensitivity 94.59%, specificity 73.81%).</p><p><strong>Conclusions: </strong>Our findings highlight the pivotal role of NLRP3 inflammasome activation and GSDMD-dependent pyroptosis in mediating poor rehabilitation outcomes in KOA. The predictive model achieved excellent performance in identifying patients at risk of suboptimal rehabilitation (AUC = 0.911, sensitivity 94.59%, specificity 73.81%).</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"124-132"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of relationship between endoscopic activity index and inflammatory indicators such as fecal calprotectin, C-reactive protein to albumin ratio, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio in ulcerative colitis patients.","authors":"Tarang Taghvaei, Hashemi Seyyed Abbas, Iradj Maleki, Mahboobe Ebrahimi, Arash Kazemi, Shahabi Reyhane Ebrahimi, Charati Jamshid Yazdani","doi":"10.5937/jomb0-58955","DOIUrl":"https://doi.org/10.5937/jomb0-58955","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the association between low-cost inflammatory biomarkers and disease severity in ulcerative colitis (UC), with a focus on predicting acute severe ulcerative colitis (ASUC) and active disease (Mayo score &gt;1).</p><p><strong>Methods: </strong>An analytical cross-sectional study was conducted on 131 patients diagnosed with UC at Imam Khomeini Hospital, Iran, between 2022 and 2024. Demographic, clinical, and laboratory data - including neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), CRP-to-albumin ratio (CAR), and faecal calprotectin - were collected. Disease activity was evaluated using the Mayo Endoscopic Score (MES). Statistical analyses included chi-square tests, ANOVA, and multivariable logistic regression adjusted for age, sex, and body mass index (BMI).</p><p><strong>Results: </strong>The mean age of participants was 45.8± 15.9 years. Elevated inflammatory markers were observed (CRP: 39.1 ± 32.6 mg/L; ESR: 44 .0± 21.5 mm/hr). Defecation frequency S6/day was significantly associated with ASUC (c2(2) = 101.10, p&lt; 0.001). After adjustment, PLR in the third quartile (14-20) was independently associated with ASUC (O R = 2.57, p= 0.034). The percentage of monocytes was significantly higher in ASUC patients (F(1, 30) = 6.52, p= 0.016). No significant associations were found for NLR or CAR. PLR also differed significantly between active and inactive UC groups (median [ IQR] : 133.87 [96.15-190.63] vs. 129.61 [102.15-209.98], p= 0.029), although its discriminatory power was limited.</p><p><strong>Conclusions: </strong>PLR and monocyte percentage may serve as accessible indicators for assessing UC severity and identifying patients at risk for ASUC. These findings support the supplementary use of routine inflammatory blood indices in the management of UC, especially in resource-limited settings.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"72-80"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The value of protein induced by vitamin K absence or antagonist-II (PIVKA) in predicting the efficacy of transhepatic arterial chemoembolisation (TACE): A retrospective study.","authors":"Qing Shi, Jingui Xu, Zhuang Zhang, Xinxin Ruan","doi":"10.5937/jomb0-59224","DOIUrl":"https://doi.org/10.5937/jomb0-59224","url":null,"abstract":"<p><strong>Background: </strong>To investigate the value of AFP combined with degamma-carboxylprothrombin (DCP), vitamin K absence or antagonist-II (PIVKA) in predicting the efficacy of transhepatic arterial chemoembolisation (TACE).</p><p><strong>Methods: </strong>The clinical data of 69 patients with hepatocellular carcinoma (HCC) who received TACE at our hospital between March 2020 and December 2024 were retrospectively analysed. Changes in the serum AFP and DCP levels of patients before TACE and after two consecutive TACE operations were analysed. The therapeutic effect of TACE was evaluated using the MRECIST criteria. The changes in AFP and DCP levels were compared with the imaging data from the same period (mRECIST criteria). The measurement data were tested for normality, and comparisons between two groups that conformed to a normal distribution were performed using two-way independent sample t-tests. The Mann-Whitney U test was used to compare normally distributed data between the two groups. The c2 test was used to compare the counting data between the two groups, and the Mann-Whitney U test was used to compare the rank-counting data between the two groups. Spearman correlation analysis was used to explore the correlation between mRECIST grade and AFP and DCP levels. The value of each index in the diagnosis of patients in the remission group was analysed by subject working characteristic curve analysis.</p><p><strong>Results: </strong>According to the mRECIST criteria, 38 patients were in the remission group, and 31 were in the nonremission group. After treatment, the AFP and DCP levels in the remission group were significantly lower than those in the non-remission group (Z=-3.366 and -4.065, P&lt;0.05). There were statistically significant differences in AAFP ADCP AAFP%, and ADCP% between the remission group and the nonremission group (Z=-4.837, -5.597, -4.210, and -5.851, respectively; P&lt;0.001). The mRECIST stage was negatively correlated with AAFP and ADCP (RS = -0.552 and -0.593, P&lt;0.001). The area under the working characteristic curve of AAFP% was 0.796, that of ADCP% was 0.912, that of AAFP% + ADCP% combined was 0.921, and that of AAFP% + ADCP% had the most significant diagnostic value.</p><p><strong>Conclusions: </strong>A combined analysis of serum AFP and DCP levels before and after TACE can be used to evaluate the therapeutic effect of TACE in patients with hepatocellular carcinoma.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"81-90"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of low molecular weight heparin on inflammatory, coagulation, and immune markers in hyperlipidemic acute pancreatitis.","authors":"Yujie Lu, Xinchao Zhu, Caixia Wen, Qiang Zhang, Li Zhao, Jia Ling","doi":"10.5937/jomb0-58836","DOIUrl":"https://doi.org/10.5937/jomb0-58836","url":null,"abstract":"<p><strong>Background: </strong>This study investigates the biochemical impact of low molecular weight heparin (LMWH) on key immunological, coagulation, and inflammatory markers in patients with hyperlipidemic acute pancreatitis (HLAP). The objective is to elucidate the role of LMWH in modulating serum immunoglobulins (IgA, IgG, IgM), coagulation parameters (PT, TT, FIB, APTT), proinflammatory cytokines (TNF-a, IL-6, IL-8), and digestive enzyme activity (amylase), thereby providing insight into its therapeutic mechanism.</p><p><strong>Methods: </strong>A total of 100 HLAP patients treated between January 2022 and December 2024 were assigned to a control group (CG) receiving standard medical treatment, and an experimental group (EG) receiving standard treatment plus LMWH. Biomolecular markers were analysed to assess changes in coagulation dynamics, inflammatory signalling, immunoglobulin response, and lipid metabolism. Comparative analysis between groups was conducted to evaluate the biochemical effects of LMWH.</p><p><strong>Results: </strong>Compared to the control group, the LMWH-treated group demonstrated significant reductions in inflammatory mediators: TNF-a decreased by 38.2%, IL-6 by 34.5%, and IL-8 by 36.7% (all P&lt;0.01). Serum amylase and urinary amylase levels declined by 41.3% and 39.6%, respectively (P&lt;0.01). Coagulation profiles improved with PT prolonged by 13.8%, TT by 15.2%, FIB reduced by 12.4%, and APTT normalised by a 17.1% increase (P&lt;0.01). Immune markers IgA, IgG, and IgM increased by 22.5%, 26.3%, and 24.8%, respectively (P&lt;0.01). Additionally, the LMWH group showed better lipid regulation (TG reduced by 45.7%) and a lower complication rate (6% vs. 22%, P=0.02).</p><p><strong>Conclusions: </strong>LMWH exhibits a multifaceted biochemical effect in HLAP patients, encompassing anti-inflammatory action, immunomodulation, and correction of coagulation abnormalities. These findings support LMWH as a potential adjunctive therapeutic agent in the biochemical management of HLAP warranting further molecular studies to explore its mechanistic pathways.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"177-189"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12980155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The serum vitamin D status in adults with diabetic retinopathy: A systematic review and meta-analysis.","authors":"Hongyu Wei, Hao Wang, Ke Diao, Xiaoxuan Wang, Hong Chen, Congying Wang, Minglian Zhang","doi":"10.5937/jomb0-60670","DOIUrl":"https://doi.org/10.5937/jomb0-60670","url":null,"abstract":"<p><strong>Background: </strong>Emerging evidence suggests that vitamin D may play a crucial role in the development of diabetic retinopathy (DR). However, the extent of vitamin D deficiency in individuals with DR remains uncertain. This study aimed to evaluate serum vitamin D levels in adults with DR.</p><p><strong>Methods: </strong>A literature was retrieved using the Embase, PubMed and Cochrane databases to identify observational studies that evaluated the levels of 25(OH)DS, 1,25(OH)2D5 or total 25(OH)D in the serum of patients with DR. A total of 31 independent studies were included for meta-analysis.</p><p><strong>Results: </strong>The pooled mean concentration of total 25(OH)D among individuals with diabetes was 16.04 ng/mL (95% CI: 15.13-16.96; I2 = 98.8%), while the mean serum level of 25(OH)D3 in those with DR was 10.68 ng/mL (95% CI: 5.94-15.41; I2 = 99.5%), both significantly below the normal reference range. The average concentration of 1,25(OH)2D3 was 31.14 pg/mL (95% CI: 24.35-37.94; I2 = 98.8%).</p><p><strong>Conclusions: </strong>The evidence from this meta-analysis indicates an association between vitamin D deficiency and an increased risk of DR.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"1-10"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges in interpreting leukocyte and nucleated red blood cell counts in neonatal hemolytic disease: A case report on hematology analyser performance.","authors":"Marina Jakšić, Lidija Banjac, Boban Banjac","doi":"10.5937/jomb0-60254","DOIUrl":"https://doi.org/10.5937/jomb0-60254","url":null,"abstract":"<p><p>We present a case of hemolytic disease of the newborn (HDN) due to maternal alloimmunisation with anti-E and anti-c antibodies, resulting in severe anaemia, respiratory insufficiency, and hyperbilirubinemia in a term male neonate. Haematological evaluation using the automated analyser Sysmex XN-3100 (Sysmex Corporation, Kobe, Japan) yielded an erroneously elevated white blood cell (WBC) count of 1 6 3 x 1 0 / L , later manually corrected to 2 8 x 1 0 / L due to extreme nucleated red blood cell (NRBC) interference (&gt; 2,000 NRBCs per 100 WBCs). This case illustrates the analytical limitations of modern haematology analysers in neonates with pronounced erythroblastosis. It emphasises the essential role of manual peripheral blood smear review and interdisciplinary clinical-laboratory correlation in ensuring diagnostic accuracy.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"217-219"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12980145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperthyroidism complicated with diabetes mellitus using the health promotion model: changes in thyroid hormones, glucose-lipid metabolism, and inflammatory markers.","authors":"Miao Tang, Jiao Xu, Yanling Chen, Bo Zhou","doi":"10.5937/jomb0-59606","DOIUrl":"https://doi.org/10.5937/jomb0-59606","url":null,"abstract":"<p><strong>Background: </strong>To investigate the effects of a Health Promotion Model (HPM)-based comprehensive intervention on thyroid hormones, glucose-lipid metabolism, and inflammatory markers in patients with hyperthyroidism (HT) complicated by diabetes mellitus (DM), and to elucidate the potential mechanisms underlying these effects.</p><p><strong>Methods: </strong>A total of 142 patients diagnosed with HT and DM between January 2024 and January 2025 were enrolled. Participants were divided into two groups: the HPM group (n = 64), which received a structured HPM-based intervention, and the conventional group (n = 78), which underwent standard management. Laboratory assessments were conducted before and after the intervention to evaluate thyroid hormones (FT3, FT4, TSH), glucose-lipid metabolism parameters (fasting plasma glucose [FPG], glycated hemoglobin [HbA1c], total cholesterol [TC], triglycerides [TG]), and inflammatory markers (high-sensitivity C-reactive protein [hs-CRP], interleukin-1p/6 [IL-1 b/IL-6]). Data were analyzed using SPSS 24.0.</p><p><strong>Results: </strong>Compared to the conventional group, the HPM group exhibited significantly greater improvements in thyroid function, with more pronounced reductions in FT3 and FT4 (P&lt; 0.001) and a greater increase in TSH (P&lt; 0.001). Regarding glucose-lipid metabolism, significantly larger decreases in FPG, HbA1c, TC, and TG were identified in the HPM group (P&lt; 0.001). Among inflammatory markers, the HPM group showed significant reductions in hs-CRP IL-1 b, IL-6, MIP-1a, and MMP-9 (P&lt; 0.001), whereas the conventional group only exhibited improvements in hs-CRP and IL-1 b (P&lt; 0.001).</p><p><strong>Conclusions: </strong>The HPM-based intervention effectively disrupts the 'thyroid-glycolipid-inflammation' axis in HT-DM comorbidity through cognitive restructuring, behavioral modification, and environmental support.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"160-167"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12980152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation risk analysis of serum inflammatory factors and 25-hydroxyvitamin D in vascular lesions in type 2 diabetes patients.","authors":"Qian Zhang, Yuyan Zhu, Hui Li, Xue Wu, Cheng Wu, Guang Li, Chao Yang","doi":"10.5937/jomb0-59322","DOIUrl":"https://doi.org/10.5937/jomb0-59322","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To explore serum inflammatory factors and 25-hydroxyvitamin D and diabetic vascular lesions in patients with T2DM.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 362 adult (aged 18 years) patients with T2DM hospitalised from April 2022 to June 2024 were selected as research subjects. A patient's body mass index (BMI), systolic blood pressure (SBP), diastolic blood pressure (DBP), age, sex, and length of illness were among the general data gathered. Blood parameters, glycated haemoglobin (HbA1c), fasting blood glucose (FPG), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), inflammatory factors [C-reactive protein (CRP), interleukin-6 (IL-6)], and 25-hydroxyvitamin D [25(OH) D] were determined. The homeostatic model assessment of insulin resistance index (HOMA-IR) and the systemic inflammatory index (SII) were computed. The vascular lesions were divided into three groups: the simple T2DM group (155 patients), the T2DM microvascular lesion group (122 patients), and the T2DM macrovascular lesion group (85 patients). One-way analysis of variance, the Kruskal-Wallis H test, or the c2 test was used for comparisons among multiple groups. The relationship between 25(OH) D and several inflammatory markers was examined using Spearman correlation analysis, and the factors influencing diabetic vascular lesions were analysed using multivariate logistic regression analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The levels of 25(OH) D in both the T2DM microvascular lesion group and the T2DM macrovascular lesion group were significantly lower than those in the simple T2DM group (P&lt;0.05). The level of IL-6 in the T2DM microvascular lesion group was significantly greater than that in the simple T2DM group (P&lt;0.05). Compared to the simple T2DM group and the T2DM microvascular lesion group, the T2DM macrovascular lesion group had significantly higher levels of CRP IL-6, and the SII (P&lt; 0.05). Multivariate logistic regression analysis results showed that after controlling for confounding variables. With the simple T2DM group as the reference, CRP (OR= 5.35, 95% CI 1.49-19.13) and 25(OH) D (OR= 0.88, 95% CI 0.78-0.98) were the influencing factors of microvascular lesions in T2DM patients; CRP IL-6, SII, and 25(OH) D were influencing factors of macrovascular lesions in type 2 diabetes mellitus (T2DM) patients (OR = 14.99, 95% CI 2.84-79.13; OR= 27.92, 95% CI 4.24-183.92; OR= 1.01, 95% CI 1.00-1.02; OR= 0.74, 95% CI 0.60-0.92). Spearman correlation analysis revealed a negative correlation between CRP and IL-6 (r= 0.600, P&lt;0.001) and between CRP and the SII (r= 0.256, P&lt;0.001), while IL-6 showed a positive correlation with the SII (r= 0.307, P&lt; 0.001). Additionally, 25(OH) D had a negative correlation with CRP (r=-0.102, P= 0.052), IL-6 (r=-0.115, P= 0.028), and the SII (r=-0.141, P=0.007).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Compared with those in patients with T2DM microvascular lesions, the levels of the ","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"91-99"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of mucosal-associated invariant T+ cells combined with inflammatory factors in differentiating chronic rhinosinusitis subtypes with or without nasal polyps.","authors":"Yilong Wang, Yongjun Feng, Jie Song, Zhonglin Mou","doi":"10.5937/jomb0-59840","DOIUrl":"https://doi.org/10.5937/jomb0-59840","url":null,"abstract":"<p><strong>Background: </strong>The present study attempted to analyze the effect of mucosal-associated invariant T (MAIT) combined with inflammatory factors in identifying chronic rhinosinusitis (CRS) without nasal polyps (CRSsNP) and CRS with nasal polyps (CRSwNP) that provides a clinical reference.</p><p><strong>Methods: </strong>In this retrospective study, we enrolled 56 CRSsNP patients, 68 CRSwNP patients, and 64 healthy controls. Flow cytometry was used to detect the frequency of peripheral blood MAIT+ cells (MAIT+). Serum levels of hs-CRP, IL-ip, and IL-6 were measured by ELISA and automatic blood cell analyzer, and their correlations with MAIT cell alterations were analyzed. The diagnostic effect of MAIT+ combined with hs-CRP IL-ip, and IL-6 on CRS was analyzed using ROC curves.</p><p><strong>Results: </strong>Both CRSsNP and CRSwNP patients exhibited significantly reduced MAIT+ frequencies compared to controls (P&lt; 0.05), with CRSwNP showing the most pronounced decrease (P &lt; 0.0 5). MAIT+ frequency was inversely correlated with hs-CRP IL-ip, and IL-6 in CRS patients. ROC analysis demonstrated that MAIT+ effectively discriminated CRSsNP (AU C= 0.793) and CRSwNP (AU C= 0.863) from healthy individuals (P&lt; 0.01). After combined with inflammatory factors, the diagnostic effect of MAIT+ combined inflammatory factors on CRSsNP (AU C= 0.933) and CRSwNP (AU C= 0.939) was further improved.</p><p><strong>Conclusions: </strong>Detection of MAIT+ combined with inflammatory factors may provide potential biomarkers for CRS subtype identification.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":"133-140"},"PeriodicalIF":1.5,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}