Journal of Medical Biochemistry最新文献

{"title":"Diagnostic levels of C-reactive protein (CRP), epinephrine (EP), cortisol (COR), and norepinephrine (NE) in patients undergoing laparoscopic surgery for gallstones receiving anti-inflammatory therapy.","authors":"Gaoqiang Wang","doi":"10.5937/jomb0-54684","DOIUrl":"10.5937/jomb0-54684","url":null,"abstract":"<p><strong>Background: </strong>The study aimed to investigate the effect of anti-inflammatory and choleretic soup on CRP, Ep, Cor, NE, and gallbladder function recovery in patients following laparoscopic cholecystectomy.</p><p><strong>Methods: </strong>A total of 111 patients with gallstones who underwent laparoscopic surgery at the hospital between November 2019 and March 2024 were selected. All patients were randomly assigned to two groups using a random number method. The control group (n=56) received extracorporeal circulation treatment alone, while the experimental group (n=55) received additional treatment with anti-inflammatory bile soup. Differences in the levels of C-reactive protein (CRP), epinephrine (Ep), cortisol (Cor), and norepinephrine (NE) were compared and analysed between the two groups. The effects on gallbladder function recovery were also evaluated.</p><p><strong>Results: </strong>Three months post-treatment, the levels of CRP, Ep, Cor, and NE in both groups had decreased compared to pre-treatment levels. Post-treatment levels of these indices were significantly lower in the experimental group than in the control group (P<0.05). Furthermore, the gallbladder wall thickness and fasting gallbladder volume (FV) in both groups were reduced compared to pre-treatment levels, while the gallbladder contraction rate improved. The experimental group exhibited better gallbladder function outcomes across various indices than the control group (P<0.05).</p><p><strong>Conclusions: </strong>The application of anti-inflammatory and choleretic soup in patients following laparoscopic cholecystectomy contributes to improvements in physiological parameters, enhances the recovery of gallbladder functional indices, and improves the quality of life during prognosis and rehabilitation.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1251-1262"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of serum CRP, IgM and IgA levels in children with Mycoplasma pneumoniae and pleural effusion.","authors":"Huiqing Guo","doi":"10.5937/jomb0-54947","DOIUrl":"10.5937/jomb0-54947","url":null,"abstract":"<p><strong>Background: </strong>Mycoplasma pneumoniae pneumonia (MPP) is a common cause of community-acquired pneumonia in children, with pleural effusion (PE) as a recognised but challenging complication. Identifying reliable biomarkers to predict PE in MPP is crucial for timely intervention. This study aims to evaluate the prognostic value of serum C-reactive protein (CRP), Immunoglobulin M (IgM), and Immunoglobulin A (IgA) levels in children with MPP and PE.</p><p><strong>Methods: </strong>A retrospective study was conducted on 200 pediatric patients diagnosed with MPP between January 2021 and December 2023. Patients were divided into two groups: MPP with PE (n=100) and MPP without PE (n=100). Serum CRP , IgM, and IgA levels were measured, and their associations with PE were analysed using logistic regression models.</p><p><strong>Results: </strong>The MPP with PE group had significantly higher CRP (30.22±24.01 mg/L vs 9.90±7.01 mg/L, P<0.001) and IgM (167.39±85.68 mg/dL vs 130.48±77.65 mg/dL, P=0.002) levels compared to the MPP without PE group. In contrast, IgA levels were significantly lower in the PE group (164.50±87.22 mg/dL vs. 195.51±79.93 mg/dL, P=0.009). Multivariate logistic regression analysis revealed that elevated CRP (OR=1.255, 95% CI: 1.132-1.391, P<0.001) and IgM (OR=1.795, 95% CI: 1.777-4.867, P=0.001) were independent risk factors for PE, while higher IgA levels were protective (OR=0.281, 95% CI: 0.131-0.602, P=0.001).</p><p><strong>Conclusions: </strong>Serum CRP and IgM levels are potential predictors of pleural effusion in children with MPP , while elevated IgA levels may indicate a lower risk. These biomarkers could aid in early risk stratification and guide clinical management to improve outcomes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1279-1287"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of prognostic risk of death in patients with multiple myeloma based on CD184 and CD269: A retrospective analysis.","authors":"Hong Chen, Yuan Zhao, Zhiyu Zhang, Yan Xie, Mulan Jin","doi":"10.5937/jomb0-56987","DOIUrl":"10.5937/jomb0-56987","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to analyze the expressions of CD184 and CD269 in patients with newly diagnosed multiple myeloma (MM) in China and explored their potential association with prognosis.</p><p><strong>Methods: </strong>This retrospective study recruited 100 patients with MM and 113 healthy controls who attended Beijing Chaoyang Hospital Shijingshan District between June 2020 and December 2023. The association between CD184 and CD269 expression and patient survival outcomes was assessed using univariable and multivariable Cox proportional hazards regression analyses, alongside Kaplan-Meier survival curves.</p><p><strong>Results: </strong>Both CD184 and CD269 mRNA were elevated in MM patients compared to controls (P<0.05). By qualitative analysis, it was seen that both CD184+ and CD269 patients had a reduced prognostic overall survival (OS) (P<0.05). Similarly, detection of CD184 and CD269 expression was effective in predicting prognostic mortality in patients.</p><p><strong>Conclusions: </strong>CD184 and CD269 may serve as valuable prognostic markers in MM patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1210-1218"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of CRP, PCT, and TNF-a, D-lactate, DAO, and endotoxin on survival of patients with severe craniocerebral injury.","authors":"Ming Gong, Han Peng","doi":"10.5937/jomb0-54673","DOIUrl":"10.5937/jomb0-54673","url":null,"abstract":"<p><strong>Background: </strong>Hyperbaric oxygen therapy is an important treatment method for early consciousness recovery and rehabilitation after brain trauma. In this study, we investigated the effects of hyperbaric oxygen combined with probiotics nutritional supplements (PNS) on patients with severe craniocerebral injury complicated with swallowing disorders in the Intensive care unit.</p><p><strong>Methods: </strong>Seventy-four patients with severe craniocerebral injury who received hyperbaric oxygen therapy in the ICU of our hospital from July 2020 to October 2023 were selected for retrospective analysis. Among them, 35 received conventional NS (control groups), and another 39 received PNS (research group). Inflammatory factors, T-lymphocyte subsets, and gastrointestinal mucosal function were compared between the two groups before and after treatment. In addition, patients' neurological function was assessed using the Glasgow Coma Scale (GCS), the Fugl-Meyer Assessment (FMA), and the National Institutes of Health Stroke Scale (NIHSS). Finally, the incidence of adverse effects between the two groups was counted.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1340-1346"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical role of homocysteine in immune modulation and cytokine dynamics in acute ischemic stroke: Implications for stroke-associated infections.","authors":"Jiayi Cai, Qiuxin Xu, Haofeng Shen","doi":"10.5937/jomb0-56695","DOIUrl":"10.5937/jomb0-56695","url":null,"abstract":"<p><strong>Background: </strong>Ischemic stroke is a leading cause of morbidity and mortality, with immune dysregulation contributing to its progression. Elevated homocysteine (Hcy) levels are implicated in altering immune responses and increasing stroke severity. This study aimed to investigate the biochemical role of serum homocysteine in modulating immune responses, particularly cytokine profiles, and its association with post-stroke infections in patients with acute ischemic stroke.</p><p><strong>Methods: </strong>A cohort of 106 patients with acute ischemic stroke was divided into Low-, Medium-, and High-Hcy groups. Serum levels of cytokines (IL-6, IL-4, IFN-g, IL-10) and immune modulation markers (e.g., IFN-g/IL-4 ratio) were quantified. The presence of stroke-associated infections (SAI) was recorded, and its relationship with immune parameters was analyzed.</p><p><strong>Results: </strong>The High-Hcy group showed significantly higher serum levels of IL-6, IFN-g, and IL-10 compared to the Low-Hcy group (P < 0.05), suggesting a pro-inflammatory bias. In patients with SAI, IL-4 levels were notably elevated, and the IFN-g/IL-4 ratio indicated an immune suppressive trend. Although stroke severity was similar across groups, those with heightened immune dysregulation were more prone to infections.</p><p><strong>Conclusions: </strong>Elevated homocysteine levels induce a shift in immune responses, emphasizing the dual role of cytokines in stroke pathophysiology. Targeting these biochemical pathways may present novel therapeutic strategies to mitigate stroke complications.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1314-1321"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory biomarkers and immunological modulation of sintilimab in gastric cancer: A meta-analysis focused on tumor markers and T-cell subsets.","authors":"Xian Zhang, Qiang Zhao, Huafei Tang, Rui Qin, Ting Tian, Congying Li, Rui Ma","doi":"10.5937/jomb0-59513","DOIUrl":"10.5937/jomb0-59513","url":null,"abstract":"<p><strong>Background: </strong>Sintilimab, a PD-1 inhibitor, has emerged as a promising immunotherapeutic agent in gastric cancer. However, its impact on laboratory-based biochemical markers and immune indicators remains underexplored. This meta-analysis aimed to evaluate the changes in tumor biomarkers and T lymphocyte subsets, alongside clinical outcomes, in patients receiving sintilimab.</p><p><strong>Methods: </strong>A comprehensive literature search of randomized controlled trials (2022-2025) was conducted across CNKI, Wanfang, VIP, and PubMed databases. Primary outcomes included serum tumor markers (CEA, CA199, CA242) and immune parameters (CD4+, CD8+ T-cell subsets). Secondary outcomes were ORR, DCR, OS, PFS, and adverse reactions. RevMan 5.2 was used for meta-analysis.</p><p><strong>Results: </strong>Sixteen studies were included. Sintilimab treatment significantly reduced CEA, CA199, and CA242 levels (P < 0.0001), and favorably modulated immune subsets by increasing CD4+ and decreasing CD8+ cell counts. These biochemical and immunological improvements correlated with higher ORR, DCR, and OS, without increased adverse events (P > 0.05).</p><p><strong>Conclusions: </strong>Sintilimab confers measurable improvements in key laboratory-based tumor and immune biomarkers, supporting its utility in clinical biochemical monitoring and immunotherapy response evaluation for gastric cancer patients. These findings align with the emerging integration of immunotherapy and biochemical diagnostics in oncology.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1171-1182"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic value of angiotensin, g-GT, blood lactate, and renal resistance index combined detection in acute kidney injury after neonatal asphyxia.","authors":"Xiaoqing Shi, Liying Dai, Ying Wang, Fang Deng","doi":"10.5937/jomb0-56995","DOIUrl":"10.5937/jomb0-56995","url":null,"abstract":"<p><p>To investigate the diagnostic value of angiotensin (Ang), gamma-glutamyltransferase (g-GT), blood lactic acid and renal resistive index (RRI) for acute kidney injury (AKI) after neonatal asphyxia.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1271-1278"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese medicine acupoint pasting for preventing and treating gastrointestinal reactions in type II diabetes mellitus patients undergoing glucagon-like peptide-1 receptor agonist therapy: A clinical study.","authors":"Songsong Zheng, Diyi Zhou, Fangfang Chen, Jiandi Zheng","doi":"10.5937/jomb0-55092","DOIUrl":"10.5937/jomb0-55092","url":null,"abstract":"<p><strong>Background: </strong>Patients with type 2 diabetes mellitus (T2DM) initiating treatment with glucagon-like peptide-1 receptor agonists (GLP-1RAs) may encounter various risks and complications.</p><p><strong>Methods: </strong>This study randomly assigned 315 patients starting GLP-1RA therapy into a control group (CG, standard treatment + routine care), a placebo group (PG, placebo + routine care), and a traditional Chinese medicine (TCM) group (TG, TCM plaster therapy + routine care). The glycemic control, pancreatic function, hematological parameters, renal function, and adverse reactions (ARs) were compared among the groups.</p><p><strong>Results: </strong>The TG exhibited no significant differences in fasting blood glucose (FBG), postprandial blood glucose at two hours, glycated hemoglobin (Hb), fasting insulin (FINS) levels, b-cell insulin secretion, and insulin resistance (IR) when compared to the CG and the PG (P>0.05). Additionally, there were no significant changes in Hb, white blood cell (WBC) count, and erythrocyte sedimentation rate (ESR) in the TG relative to the CG and PG (P>0.05). Renal function indicators revealed that the levels of blood urea nitrogen (BUN) and serum creatinine (Cr) in the TG did not differ significantly from those in the CG and the PG (P>0.05). The incidence of ARs in TG (8.57%) was markedly lower than in PG (17.14%) and CG (18.1%) (P<0.05).</p><p><strong>Conclusions: </strong>The application of TCM Liangfu Pills via acupoint plaster did not demonstrate significant therapeutic effects on glycemic control, pancreatic function, or routine blood parameters. However, it was effective in significantly reducing the risk of gastrointestinal adverse reactions associated with GLP-1RA therapy.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1331-1339"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress modulation in type 2 diabetes: Insights from metformin and pioglitazone therapy.","authors":"Seshadri Reddy Varikasuvu","doi":"10.5937/jomb0-59850","DOIUrl":"10.5937/jomb0-59850","url":null,"abstract":"<p><p>This commentary critically examines a recent study that assessed the effects of metformin and pioglitazone on oxidative stress in patients with type 2 diabetes mellitus. The original study utilized multiple biomarkers such as IMA, TOS, TAS, and OSI but observed only limited antioxidant effects and a rise in IMA levels. By referencing additional clinical trials, experimental studies, and reviews, this commentary emphasizes the importance of using broader biomarker panels, extending treatment duration, and interpreting oxidative stress markers with caution in diabetes research.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1386-1388"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of miR-4465 targeting PTEN-mediated autophagy of astrocytes in epilepsy.","authors":"Jinhua Zhao, Jihong Tang, Xiaoyan Shi","doi":"10.5937/jomb0-52626","DOIUrl":"10.5937/jomb0-52626","url":null,"abstract":"<p><strong>Background: </strong>To investigate the mechanism of miR-4465 targeting PTEN-mediated autophagy of astrocytes in epilepsy.</p><p><strong>Methods: </strong>Serum samples were collected from epileptic children and healthy children. Extract foreign bodies from serum samples and determine their quality. The exosomes were sequenced, and the abnormal expression of miRNA in patients' serum exosomes was analysed, and the expression of miR-4465 was verified by quantitative PCR. Bioinformatics predicts the size of miR-4465 and makes GO and KEGG analyses. HEK293 cells were cultured, and the relationship between miR-4465 and its target was detected using the double luciferase reporting method. Astrocytes were cultured, and quantitative PCR and WB were used to detect the expression of miR-4465 and PTEN after overexpression. In addition, CCK-8 and WB were used to detect the growth of miR-4465 and the changes of autophagy-related proteins ATG5 and Beclin1, respectively.</p><p><strong>Results: </strong>miR-4465 was markedly increased in exosomes. The bioinformatic analysis found the differentially expressed target genes of miR-4465 were mainly enriched in molecular binding, molecular function regulation, and other molecular functions and participated in cell adhesion, cell-extracellular matrix receptor interaction, and the Rap1 signalling pathway. PTEN has been predicted as a target gene of miR-4465; meanwhile, the results of the dual-luciferase reporter assay confirmed the interaction between miR-4465 and PTEN. Quantitative PCR, as well as WB results, suggested the level of PTEN was decreased in serum exosomes of patients with epilepsy, while increased miR-4465 expression inhibited expressions of PTEN. CCK-8, as well as WB results, suggested miR-4465 could suppress the growth of astrocytes and promote ATG5 as well as Beclin1 expression; finally, up-regulation of PTEN partially alleviated effects of miR-4465 on astrocytes growth as well as autophagy.</p><p><strong>Conclusions: </strong>In children with epilepsy, miR-4465 can target and regulate PTEN to promote autophagy in astrocytes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1347-1356"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}