Journal of Medical Biochemistry最新文献

{"title":"Serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor.","authors":"Ginka Delcheva, Katya Stefanova, Teodora Stankova, Ana Maneva","doi":"10.5937/jomb0-44758","DOIUrl":"10.5937/jomb0-44758","url":null,"abstract":"<p><strong>Background: </strong>Thioredoxin (Trx) and lactoferrin (Lf) are multifunctional proteins that are part of the body's antioxidant defence and counteract oxidative tissue damage. Our study aims to investigate Trx and LF levels in the serum of rheumatoid arthritis (RA) patients and establish the association of these proteins with the rheumatoid factor (RF) and other disease markers.</p><p><strong>Methods: </strong>The study included 114 patients with RA and 42 healthy subjects. Serum concentrations of Trx 1, LF, RF, anti-cyclic citrullinated peptide (anti-CCP) antibodies, Creactive protein (CRP), and IL-6 were determined using commercially available ELISA kits.</p><p><strong>Results: </strong>Serum thioredoxin 1 levels in RA were significantly higher compared to the control group, 36.4 (29.6-40.2), ng/mL versus 19.0 (16.3-26.8), ng/mL, p<0.0001. Serum lactoferrin levels were elevated in RA compared to the control group. However, the difference was not statistically significant, 579.6 (312.8-947.5) ng/mL versus 519.0 (262.5-928.0) ng/mL. We found substantial negative Trx 1 and LF correlations with rheumatoid factor in RA (r=-0.254, p=0.05 and r=-0.238, p=0.014, respectively). The correlations of thioredoxin and lactoferrin with other disease markers, such as anti-CCP antibodies, DAS28, erythrocyte sedimentation rate (ESR), CRP, and IL6 were not statistically significant. A strong positive correlation between Trx 1 and LF was observed in the study group of RA patients (r=0.519, p<0.0001) but not in the control group.</p><p><strong>Conclusions: </strong>Thioredoxin and lactoferrin were associated with rheumatoid factor but not with anti-CCP antibodies and systemic disease activity; therefore, the two proteins may serve as new biomarkers for assessing pathological changes and monitoring disease severity and progression in RA.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"312-318"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shear wave elastography to evaluate the effect of serum uric acid levels on carotid artery elasticity in patients with high-normal blood pressure.","authors":"Yifan Wang, Xinyu Wang, Xiya Li, Zhen Li, Mengmeng Zhang, Siran Zhang, Le Ma, Dongmei Huang, Guangsen Li","doi":"10.5937/jomb0-52077","DOIUrl":"10.5937/jomb0-52077","url":null,"abstract":"<p><strong>Background: </strong>Populations with normal high blood pressure often already have elevated blood uric acid levels and a higher chance of developing atherosclerosis, which accelerates the process of cardiovascular disease. The risk of atherosclerosis increases with thickening of the carotid artery intima-media, and changes in carotid artery elasticity precede intima-media changes, so early monitoring of carotid artery elasticity is of great significance in the prevention of cardiovascular disease. Using shear wave elastography (SWE) to evaluate the effect of serum uric acid (SUA) levels on carotid artery elasticity in patients with high-normal blood pressure (BP).</p><p><strong>Methods: </strong>One hundred and fifteen patients with high-normal BP were selected, and then divided into tertiles according to the SUA levels. The left carotid intima-media thickness (IMT), peak systolic velocity (PSV) and diameter of common carotid artery were measured by two-dimensional ultrasound and Doppler flow imaging. Longitudinal elasticity of the left anterior carotid wall was measured by SWE, including the mean values of the minimum elastic modulus (MEmin), maximum elastic modulus (MEmax) and mean elastic modulus (MEmean).</p><p><strong>Results: </strong>IMT, MEmean, MEmin and MEmax were obviously higher in the 3rd tertile (all P < 0.05), while there were no obviously different between the 1st tertile and 2nd tertile. Pearson correlation analysis showed positive correlations between SUA and SWE-related parameters, while there was no correlation with IMT. Multiple linear regression analysis found that age, systolic BP, and SUA levels were independently associated with SWE-related parameters.</p><p><strong>Conclusions: </strong>SWE will be more helpful than IMT in monitoring the effect of blood uric acid levels on carotid artery elasticity in patients with high-normal BP.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"227-234"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can pretreatment lactate dehydrogenase to albumin ratio predict pathological complete response after neoadjuvant chemotherapy in breast cancer patients?","authors":"Gözde Savaş, Nazan Günel, Ahmet Özet","doi":"10.5937/jomb0-43900","DOIUrl":"10.5937/jomb0-43900","url":null,"abstract":"<p><strong>Background: </strong>This study aims to evaluate the predictive significance of platelet lymphocyte ratio (PLR), neutrophillymphocyte ratio (NLR), lymphocyte monocyte ratio (LMR), systemic immune-inflammation (SII), prognostic nutritional index (PNI), haemoglobin, albumin, lymphocyte, and platelet (HALP) score and lactate dehydrogenase to albumin ratio (LAR) for pCR in breast cancer with neoadjuvant chemotherapy (NACT).</p><p><strong>Methods: </strong>A total of 121 patients who received NACT between February 2012 and November 2021 were included. LAR, NLR, PLR, MLR, SII, PNI and HALP were calculated using formulas. The cut-off value for markers was obtained by Receiver operating characteristic curve (ROC) analyses. Independent predictive factors for pCR were determined using multivariate regression analysis.</p><p><strong>Results: </strong>The pCR rate was achieved in 31.4% of patients. Median values of NLR, PLR, MLR, SII, PNI and HALP were similar in pCR (+) and pCR (-) (p>0.05). The median LAR value was significantly higher in pCR (+) than in pCR (-) (50.80 vs 42.62, respectively (p=0.002)). The optimal cut-off value of LAR was 46.27. Multivariate analysis showed that LAR ≥46.27 and HER-2 positivity were the independent predictive factors for pCR [OR=2.851 (95% CI=1.142-7.119, P=0.025), OR=3.431 (95% CI= 0.163-10.123, P=0.026), respectively].</p><p><strong>Conclusions: </strong>LAR is a simple, inexpensive, and convenient method for predicting pCR in breast cancer with NACT.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"339-346"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pituitary cells in man during aging: An immunohistological and morphometric study.","authors":"Jovana Čukuranović-Kokoris, Braca Kundalić, Miljana Pavlović, Sladjana Ugrenović","doi":"10.5937/jomb0-54605","DOIUrl":"10.5937/jomb0-54605","url":null,"abstract":"<p><strong>Background: </strong>Aging is a multifactorial process, which inevitably leads to numerous changes in the life of each individual. As the lifespan of people around the world has increased, scientists began to study the mechanisms that would enable high-quality and safe aging. In this review, the focus is on showing the effect of aging on the characteristics of pituitary cells.</p><p><strong>Methods: </strong>In order to write this review, we undertook an electronic literature search through the PubMed and Google Scholar databases using the terms aging, histological, ultrastructure of pituitary cells, also using the \"related articles\" option. We also analyzed authored and co-authored articles, which were necessary for this type of publication.</p><p><strong>Results: </strong>This review article describes the immuno-histological, ultrastructural and morphometric characteristics of adrenocorticotropic (ACTH), somatotropic (GH), gonadotropic (LH) and folliculostellate (FS) pituitary cells during aging, in young (about 47 years) and old (over 70 years) human cadavers.</p><p><strong>Conclusions: </strong>The described immunohistological and morphometric characteristics of pituitary cells in this review showed that the volume density of ACTH, GH and LH cells was statistically increased (p<0.05) in old cadavers, which indicates an attempt by these cells to bring the pituitary gland to a relatively balanced state.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"203-210"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive value of pre-delivery serum b-human chorionic gonadotropin, fibrinogen, and homocysteine for pregnancy-induced hypertension.","authors":"Hani Lin, Hong Chen, Miaomiao Zhuo","doi":"10.5937/jomb0-52483","DOIUrl":"10.5937/jomb0-52483","url":null,"abstract":"<p><strong>Background: </strong>We aimed to investigate the relationship between pre-delivery serum b-human chorionic gonado - tropin (b-HCG), fibrinogen (FIB), and homocysteine (HCY) with hypertensive disorder complicating pregnancy (HDCP).</p><p><strong>Methods: </strong>This was a case-control study. 200 HDCP patients and 150 normal pregnant women were selected as study subjects. Fasting cubital venous blood samples were collected to measure serum triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL), and lowdensity lipoprotein (LDL), as well as b-HCG, FIB, and HCY levels. Pearson correlation analysis examined the relationship between b-HCG, FIB, HCY, and HDCP. Receiver operating characteristic (ROC) curve analysis evaluated the predictive value of these indicators for HDCP. Multiple logistic regression analysis identified risk factors (RFs) for HDCP.</p><p><strong>Results: </strong>Serum TG, TC, HDL, LDL, FIB, b-HCG, and HCY were greatly elevated in the HDCP group versus the control group (CG) (P<0.05). Serum b-HCG, FIB, and HCY showed notable positive correlations with HDCP (r=0.935, 0.547, 0.811; P<0.05), and the areas under the ROC curve (AUC) for predicting HDCP based on serum b-HCG, FIB, HCY, and their combination were 0.743, 0.659, 0.801, and 0.886, respectively (P<0.05). Lipid indicators and serum levels of FIB, b-HCG, and HCY were RFs for HDCP.</p><p><strong>Conclusions: </strong>Pregnant women with HDCP exhibited markedly elevated serum lipid levels and FIB, b-HCG, and HCY levels before delivery, which can serve as predictive indicators for HDCP.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"235-243"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expressions of shox2, RASSF1A and PTGER4, and the relationship between their methylation and clinicopathological characteristics in patients with lung cancer.","authors":"Haitao Su, Qiaoye Lv, Limei Wang, Jigang Hu, Yinggang Lv, Liting Jia, Qingbin Qi","doi":"10.5937/jomb0-54540","DOIUrl":"10.5937/jomb0-54540","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To explore the expressions of short stature homobox2 (SHOX2), Ras-association domain family 1A (RASSF1A) and prostaglandin E receptor 4 (PTGER4), and the relationship between their methylation and clinicopathological characteristics in patients with lung cancer (LC).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The surgical specimens of cancer tissues and para-carcinoma tissues were collected from 50 patients with LC in the Affiliated Hospital of Hebei University of Engineering between January and November 2023. The expressions of SHOX, RASSF1A and PTGER4 proteins in cancer tissues and para-carcinoma tissues were detected by immunohistochemistry, and methylation status of SHOX, RASSF1A and PTGER4 genes in peripheral venous blood was detected by sulfite-modified real-time fluorescence quantification. The positive expression rates of SHOX2, RASSF1A and PTGER4, and positive rates of SHOX2, RASSF1A and PTGER4 genes methylation in cancer tissues and para-carcinoma tissues were compared. The relationship between SHOX2, RASSF1A, PTGER4 methylation and clinicopathological characteristics in LC patients was compared by real-time fluorescence quantitative PCR.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The positive expression rates of SHOX2, RASSF1A and PTGER4 in cancer tissues were 44.0%, 54.00% and 50.00%, significantly lower than those in para-carcinoma tissues (90.00%, 96.00%, 82.00%, P&lt;0.05). The methylation positive rates of SHOX2, RASSF1A and PTGER4 genes in cancer tissues were 48.00%, 32.00% and 64.00%, significantly higher than those in para-carcinoma tissues (16.00%, 4.0%, 14.00%, P&lt;0.05). In terms of different pathological types and TNM staging, methylation positive rates of SHOX2 and RASSF1A genes were the highest in patients with adenocarcinoma and TNM staging at stage IV. However, since the study included a limited sample size and only two major histological subtypes of LC, caution is needed in generalizing these findings across all stages and types of LC. Larger, more diverse studies are needed to determine if these findings can be broadly applied to other LC subtypes and stages. In terms of different pathological types, methylation positive rate of PTGER4 gene was the highest in patients with TNM staging at stage IV (P&lt;0.05). While these results indicate a correlation between PTGER4 methylation and advanced disease stage, further research with a larger, more heterogeneous patient population is necessary to validate whether these findings hold true across all stages and histological subtypes of LC.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The low expressions and high methylation of SHOX2, RASSF1A and PTGER4 are common in LC patients. However, it is important to note that the small sample size and the cross-sectional nature of this study limit the ability to generalize these findings to a broader population. Additionally, the lack of longitudinal data means we cannot assess how methylation changes over time or in response to treatment, whi","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"211-220"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypophosphatemia: Unraveling a lethal connection with icu mortality in critically ill COVID-19 patients: a multicenter observational study.","authors":"Şahin Temel, Pervin Hanci, Akbudak Ismail Hakkı, Burçin Halaçli, Göksel Güven, Yeliz Bilir, Yüksel Recep Civan, Ezgi Özyilmaz, Neriman Defne Altintaş, Leyla Ferliçolak, Emre Aydin, Türkay Akbaş, Ali Ümit Esbah, Zuhal Güllü, Kamil Inci, Gülseren Elay, Arzu Topeli, Kürşat Gündoğan","doi":"10.5937/jomb0-52474","DOIUrl":"10.5937/jomb0-52474","url":null,"abstract":"<p><strong>Background: </strong>Despite a lack of sufficient knowledge about the prevalence and impact of hypophosphatemia in critically ill COVID-19 patients, organ dysfunction, adverse clinical outcomes, and increased mortality have been consistently associated with hypophosphatemia across diverse patient populations. This retrospective, observational study aimed to investigate hypophosphatemia (HypoP) frequency and establish the correlation between variations in serum phosphorus levels and outcomes in critically ill patients with SARS-CoV-2.</p><p><strong>Methods: </strong>The research comprised 205 patients diagnosed with COVID-19 confirmed via RT-PCR. The study included COVID-19 patients who experienced respiratory failure and were in intensive care for more than 24 hours, and their phosphorus values were accurately documented. Clinical para meters, comorbidities, respiratory support requirements, and laboratory findings were analysed.</p><p><strong>Results: </strong>The study participants had a median age of 64 (IQR: 54-75 years), with hypertension being the most pre - valent chronic disease (46%). During the first three days of intensive care, 33% of the participants received conventional oxygen support, whereas 54% required intubation and mechanical ventilation (MV). During this period, hypophosphatemia was noted in 25% of patients, with an ICU admission median serum phosphorus level of 1.02 (0.87-1.25) mmol/L. The median duration of stay in the intensive care unit (ICU) was 7 days, significantly extended in patients with hypophosphatemia (p=0.046). Phosphorus levels on the third day of ICU stay were an independent predictor of ICU mortality. (COX, HR=1.48, 95% CI=1.11-1.98, p=0.006).</p><p><strong>Conclusions: </strong>During the first three days of ICU admission, 25% of SARS-CoV-2 critically ill adult patients presented with hypophosphatemia. This condition was found to increase ICU mortality rates and prolong ICU stays. Therefore, it is crucial to monitor serum phosphorus levels in the care of critically ill COVID-19 patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"330-338"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of glycemic gap on prognosis and complications in vulnerable period of acute heart failure.","authors":"Lin Zheng, Weifeng Zheng, Mingming Zhang, Bo Li","doi":"10.5937/jomb0-52619","DOIUrl":"10.5937/jomb0-52619","url":null,"abstract":"<p><strong>Background: </strong>To investigate the effect of glycemic gap on the prognosis and complications of heart failure in patients with acute heart failure.</p><p><strong>Methods: </strong>A total of 100 patients with acute heart failure admitted to our hospital from January 2022 to August 2023 were retrospectively selected, and the patients were divided into two groups according to their prognosis, the good prognosis group (n=58) and the poor prognosis group (n=42). The general data of the two groups were compared, and the influencing factors on the prognosis of patients with acute heart failure during the fragile period were analyzed by multi-factor Logistics regression. ROC analyzed the predictive value of blood sugar gap on the prognosis of acute heart failure patients during the vulnerable period of heart failure, and compared the incidence of complications between the two groups of patients..</p><p><strong>Results: </strong>The blood glucose gap, NT-proBNP, Ang II, hscTn, and SCR in patients with good prognosis were lower than those in patients with poor prognosis, and LVEF and hemoglobin were higher than those in patients with poor prognosis (P<0.05). Multivariate Logistics regression analysis results showed that blood glucose gap, NTproBNP, AngII, hs-cTn, SCR, LVEF, and hemoglobin were independent influencing factors on the prognosis of acute heart failure patients during the vulnerable period of heart failure (P<0.05), ROC analysis results showed that the area under the curve of the value of blood sugar gap in the prognosis of acute heart failure patients during the vulnerable period of heart failure was 0.6071,(95% CI: 0.3107~0.9035), Youden=0.89, at this time, the sensitiv ity was 92.8 and the specificity was 97.1. The complication rate in the good prognosis group (1.72%) was significantly lower than that in the poor prognosis group (16.67%).</p><p><strong>Conclusions: </strong>Glycemic gap is related to the prognosis and complications of heart failure in patients with acute heart failure. Hyperglycemia gap will affect the prognosis of heart failure in patients with acute heart failure, resulting in poor prognosis and increasing the incidence of complications.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"221-225"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic value of microrna-499 in acute myocardial infarction: A systematic review and meta-analysis.","authors":"Hong Du, Zhiyuan Zhang, Feifei Yan, Zichao Dong","doi":"10.5937/jomb0-52617","DOIUrl":"10.5937/jomb0-52617","url":null,"abstract":"<p><strong>Background: </strong>The objective of this study was to assess the diagnostic efficacy of MicroRNA-499 in cases of acute myocardial infarction.</p><p><strong>Methods: </strong>On May 6, 2023, four electronic databases PubMed, Embase, Web of Science, and Cochrane Library were searched with no time restriction. Quality assessment was conducted using Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2). Random-effects metaanalysis was employed to combine sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR). The evaluation of publication bias was conducted through the utilization of Deeks' asymmetry test on the funnel plot.</p><p><strong>Results: </strong>The studies that were included in the analysis, encompassing sample sizes ranging from 63 to 1155, exhibited a pooled sensitivity of 0.88 and a specificity of 0.97. The pooled PLR was determined to be 29.78, while the NLRwas found to be 0.13. Furthermore, the pooled DOR was calculated to be 236.10. The area under the curve (AUC) was reported as 0.98.</p><p><strong>Conclusions: </strong>MicroRNA-499 exhibits high sensitivity and specificity for diagnosing Acute Myocardial Infarction, indicating its potential as a valuable diagnostic biomarker.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"193-202"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gender differences in correlation of biochemical parameters with the severity of covid pneumonia and the need for oxygen/mechanical support.","authors":"Jelena Jankovic, Mihailo Stjepanovic, Nikola Maric, Slobodan Belic","doi":"10.5937/jomb0-49377","DOIUrl":"10.5937/jomb0-49377","url":null,"abstract":"<p><strong>Background: </strong>The COVID-19 pandemic caused global medical, economic and social problems. High infection rates, heterogeneous presentation, lack of previous data, and lack of standardized treatment led to a need for further analysis to prepare for potential new pandemics. We analyzed any possible correlation between gender, laboratory findings, disease severity and the need for oxygen or mechanical ventilation support.</p><p><strong>Methods: </strong>99 patients with confirmed SARS-CoV-2 virus infection enrolled. Baseline characteristics that included age, sex, smoking history, BMI, oxygen therapy or mechanical ventilation support needs were recorded. Type and severity of radiological findings determined by chest CT scan.</p><p><strong>Results: </strong>The majority of our patients were over 61 years old (58.6%), male (57.6%), and had severe radiological findings (bilateral pneumonia 29.3%, ARDS 35.4%), with only 20.2% had not required any oxygen supplementation. Regarding gender and laboratory findings, men have shown statistically significant higher values of CRP, lymphocytes, LDH and ferritin (96.4 vs 87.1, p=0.014; 1.17 vs 0.84, p=0.048; 674.8 vs 609.1, p=0.031; 1263 vs 578.4, p=0.001, respectfully). Severe radiological findings showed a positive correlation with the need for HFNC and/or (N)IMV (p=0.021 and p=0.032. respectfully), as well as with higher values of WBC, LDH and ferritin (p=0.042, p=0.035 and p=0.017, respectively).</p><p><strong>Conclusions: </strong>There is a difference between the presentation of the disease and analyzed laboratory markers between sexes. The difference is most likely multifactorial and should require further research in order to discover other risk and prognostic factors.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 2","pages":"250-255"},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}