Journal of Medical Biochemistry最新文献

{"title":"Effect Of treatment on serum C3, C4, IL-10, IL1-β, IL-6, TNF-α in patients with systemic lupus erythematosus.","authors":"Nadira Reyimu, Yueyue Jing","doi":"10.5937/jomb0-62357","DOIUrl":"https://doi.org/10.5937/jomb0-62357","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the effects of structured lifestyle optimisation, including nutritional intake and exercise training, on serum levels of Complement C3, C4, IL-10, IL1-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">β</span>, IL-6, and TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> in patients with systemic lupus erythematosus (SLE) and Libman-Sacks endocarditis (LSE).</p><p><strong>Methods: </strong>A total of 122 SLE patients with LSE, treated at our hospital from May 2022 to May 2024, were randomised into a control group (CG, n = 61) receiving conventional medical interventions and an intervention group (IG, n = 61) receiving additional personalised exercise and nutritional interventions for 24 weeks. Serum levels of Complement C3, C4, IL-10, IL1-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">β</span>, IL-6, and TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> were measured at baseline, 3 months, and 6 months. Secondary endpoints included nutritional status (as measured by albumin and haemoglobin levels) and patient compliance.</p><p><strong>Results: </strong>Of 113 patients completing the study, the intervention group (IG) showed significant improvement in serum Complement C3 (0.84 ± 0.18 g/L vs. 0.75± 0.16 g/L in the control group [CG], P= 0.006) and albumin levels (37.12± 5.37 g/L vs. 35.00 ± 4.89 g/L, P= 0.03) at 6 months. Complement C4 levels increased in the IG (0.20 ± 0.09 g/L vs. 0.17± 0.12 g/L, P= 0.136, not significant). IL-1<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">β</span> (3 .9 ± 3 .7 7 pg/mL vs. 4.6± 4.1 pg/mL, P= 0.468) and TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> (12.6± 4.1 pg/mL vs. 14.1 ±5.2 pg/mL, P= 0.092) showed non-significant reductions in the IG compared to the CG. No significant changes were observed in IL-10 or IL-6 levels. Compliance was significantly higher in the IG (92.86% vs. 78.95% , P= 0.038).</p><p><strong>Conclusions: </strong>Structured lifestyle optimisation significantlyimproved serum Complement C3 and albumin levels andshowed non-significant trends toward reductions in IL-1<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">β</span> and TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> in SLE patients with LSE, suggesting a potentialadjunctive role in managing immune and nutritional status.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1383-1395"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applicability of LDL-cholesterol calculation formulas in hypertriglyceridemia: Insights from the Vojvodina.","authors":"Dragana Žuvić, Stanislava Nikolić, Romana Mijović, Branislava Ilinčić, Aneta Ranđelović-Živković, Dušan Sedlarević, Velibor Čabarkapa","doi":"10.5937/jomb0-61948","DOIUrl":"10.5937/jomb0-61948","url":null,"abstract":"<p><strong>Background: </strong>Low-density lipoproteins cholesterol (LDL-C) is a key parameter for assessing the risk of atherosclerotic cardiovascular disease. Direct measurement of LDL-C is not always possible due to practical or financial reasons, making the use of calculation formulas essential. The aim of this study was to evaluate the applicability of four different formulas proposed by Friedewald, Sampson, Anandaraja, and Martin-Hopkins for calculating LDL-C compared to direct method in patients with serum triglyceride levels from 4.5 to 9.0 mmol/L in the population of Vojvodina.</p><p><strong>Methods: </strong>The retrospective study included 272 subjects whose lipid status parameters were measured between June 2022 and June 2023. LDL-C was determined by the direct method (d-LDL-C) on the Alinity c analyser (Abbott Laboratories, Illinois, USA). Calculated LDL-C values were obtained using the four selected formulas.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1354-1363"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum biochemical and inflammatory biomarker profiles in severe preeclampsia and their clinical predictive value.","authors":"Fan Yang, Yanping Liu, Yanyan Liu","doi":"10.5937/jomb0-63709","DOIUrl":"10.5937/jomb0-63709","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia is a pregnancy-specific hypertensive disorder characterized by systemic endothelial dysfunction, metabolic disturbances, and inflammatory activation. However, comprehensive biochemical and inflammatory biomarker profiles associated with disease severity, particularly severe preeclampsia, remain insufficiently defined. This study aimed to investigate serum biochemical and inflammatory markers in preeclampsia and to evaluate their clinical predictive value for severe disease.</p><p><strong>Methods: </strong>In this retrospective case-control study, pregnant women were classified into severe preeclampsia (n=30), mild preeclampsia (n=30), and normal pregnancy control groups (n=30). Serum biochemical parameters, including alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase (LDH), uric acid, creatinine, albumin, total bilirubin, triglycerides, and total cholesterol, were measured. Inflammatory markers, including C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-8, tumor necrosis factor-a, procalcitonin, neutrophil-to-lymphocyte ratio, and platelet-to-lymphocyte ratio, were analyzed. Group comparisons, Spearman correlation analyses, multivariable logistic regression, and receiver operating characteristic (ROC) curve analyses were performed.</p><p><strong>Results: </strong>Both biochemical and inflammatory markers showed significant stepwise alterations across the three groups, with the most pronounced abnormalities observed in severe preeclampsia (P &lt; 0.05). LDH, uric acid, CRP, IL-6, and albumin were significantly associated with disease severity. Multivariable logistic regression identified elevated LDH, uric acid, CRP IL-6, and reduced albumin as independent predictors of severe preeclampsia. ROC analysis demonstrated that the combined biomarker model achieved good discriminative performance, with an area under the curve of 0.89 (95% CI: 0.81-0.96).</p><p><strong>Conclusions: </strong>Severe preeclampsia is characterized by distinct serum biochemical and inflammatory biomarker profiles. An integrated biomarker-based model may serve as a practical tool for early identification and risk stratification of severe preeclampsia.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1316-1322"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of auto-verification on turnaround time for clinical chemistry and immunoassay tests.","authors":"Havva Yasemin Çinpolat","doi":"10.5937/jomb0-61380","DOIUrl":"10.5937/jomb0-61380","url":null,"abstract":"<p><strong>Background: </strong>Auto-verification is increasingly recognised as a key tool for improving quality and efficiency in clinical laboratories. This study aimed to investigate the impact of an auto-verification system implemented for clinical chemistry and immunoassay tests on turnaround time (TAT) in a tertiary-care medical biochemistry laboratory.</p><p><strong>Methods: </strong>This study was conducted in the Medical Biochemistry Laboratory of Çanakkale Onsekiz Mart University Hospital, a tertiary healthcare institution. Clinical chemistry and immunoassay tests were automated and verified using the navify® Lab Operations middleware (Roche Diagnostics, Germany), integrated with the MIA-MED Laboratory Information System (MIA Technology, Türkiye). Algorithms were developed in accordance with CLSI AUTO10-A and AUTO 15 guidelines, incorporating rules for quality control, serum indices, analyser flags, delta checks, critical values, consistency checks, and analytical measurement intervals, as well as recommendations from the national health authorities. Validation of the algorithms was carried out using both simulated and patient data. The proportion of results exceeding predefined TAT targets was compared before and after auto-verification implementation using the chi-square test. p&lt; 0.05 was considered statistically significant.</p><p><strong>Results: </strong>Overall, 71% of test results and 21% of tube-based results were verified automatically. Median TAT was reduced by 6 minutes for emergency tests and 12 minutes for routine tests. The proportion of results exceeding the TAT threshold decreased significantly from 6.4% before auto-verification to 5.8% after auto-verification implementation (p&lt; 0.001).</p><p><strong>Conclusions: </strong>Auto-verification, with clearly defined and validated rules, enhances both the reliability and timeliness of laboratory results, thereby supporting quality improvement initiatives in clinical laboratories.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1342-1353"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of thrombospondin-1 (THBS1) and toll-like receptor 4 (TLR-4) levels in the serum and synovial fluid of patients with knee osteoarthritis.","authors":"Yingjie Xu, Tian Zhen, Liang Mingjin, Xiaoyun Li","doi":"10.5937/jomb0-62485","DOIUrl":"https://doi.org/10.5937/jomb0-62485","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To explore the changes in and significance of Thrombospondin-1 (THBS1) and Toll-like receptor 4 (TLR-4) levels in the serum and joint fluid of patients with knee osteoarthritis (KOA).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;184 KOA patients admitted to our hospital between May 2022 and February 2025 were selected as the observation group, and 64 healthy adults who underwent physical examinations at the same hospital during the same period composed the control group. Tumour necrosis factor-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt; (TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;), interleukin (IL)-1 b, THBS1, and TLR-4 levels in the two groups' serum and joint fluid were compared. The correlations between TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, IL-1 b, THBS1, and TLR-4 levels in the serum and synovial fluid of the two groups and the severity of KOA were evaluated, and the clinical value of THBS1 and TLR-4 in the diagnosis of KOA was analysed.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, IL-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, THBS1, and TLR-4 levels in the observation group's serum and joint fluid were considerably higher than those in the control group (P&lt; 0.05). With increasing Kellgren-Lawrence (K-L) grade, the observation group's serum and joint fluid showed higher levels of TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, IL-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, THBS1, and TLR-4. When TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, IL-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, THBS1, and TLR-4 levels were compared among K-L grades, statistically significant differences were found (P&lt; 0.05). The K-L grade of the patients was positively correlated with TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, IL-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, THBS1, and TLR-4 in the blood and joint fluid (P&lt; 0.05), and the factors of TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1404-1411"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of serum BAFF, CFB, MCP-1 and anti-PLA2R antibodies in the efficacy evaluation of patients with membranous nephropathy.","authors":"Kaiyuan Zheng, Tongxin Qu, Lili Han, Yi Sun","doi":"10.5937/jomb0-61102","DOIUrl":"https://doi.org/10.5937/jomb0-61102","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Monocyte chemoattractant protein-1 (MCP-1), serum B-cell activating factor (BAFF), complement B factor (CFB), and anti-M-type phospholipase A2 receptor (PLA2R) antibodies are investigated in relation to membranous nephropathy (MN), and their roles in evaluating treatment efficacy are assessed.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;108 patients who were hospitalised to our hospital between January 2023 and June 2024 were chosen to serve as research participants. Patients were divided into remission and nonremission groups based on clinical efficacy. The levels of serum BAFF, CFB, MCP-1, and anti-PLA2R antibodies in patients with positive and negative anti-PLA2R and at different pathological stages were compared, and the clinical data of the remission and nonremission groups were compared. The association between serum levels of BAFF CFB, and MCP-1 and anti-PLA2R antibody levels in patients who tested positive for anti-PLA2R antibody. The predictive efficacy of serum anti-PLA2R antibodies, BAFF, CFB, and MCP-1 for nonremission after therapy in MN patients was evaluated using receiver operating characteristic (ROC) curves.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;There were 78 patients who were positive for anti-PLA2R antibodies and 30 patients who were negative for anti-PLA2R antibodies. The levels of serum BAFF CFB, MCP-1 and anti-PLA2R antibodies in patients positive for anti-PLA2R antibodies were significantly greater than those in patients negative for anti-PLA2R antibodies (P&lt; 0,05). The comparison of serum BAFF CFB, and MCP-1 levels in MN patients at different stages was as follows: stage I &lt; stage II &lt; stage III &lt; stage IV Serum BAFF, CFB, and MCP-1 in patients positive for anti-PLA2R antibodies were positively correlated with the level of anti-PLA2R antibodies (r= 0.792, 0.823, 0.832, P&lt; 0.001). Spearman correlation analysis revealed that serum BAFF, CFB, and MCP-1 levels in MN patients were positively correlated with pathological stage (rs= 0.758, 0.752, and 0.717, respectively; P&lt; 0.001). The pathological stage and levels of anti-PLA2R antibody, BAFF, CFB, and MCP-1 were significantly higher in the nonremission group than in the remission group (P&lt; 0.05). After treatment, elevated serum levels of MCP-1, CFB, and BAFF were risk factors for nonremission in MN patients (P&lt; 0.05). The ROC curve analysis revealed that the area under the curve for the combined prediction of nonremission in MN patients after treatment with serum BAFF, CFB and MCP-1 was 0.948, which was greater than the area under the curve for the individual prediction of anti-PLA2R antibody and BAFF, CFB and MCP-1 (Z = 4.116 , 3.059, 4.122, 4.116, P&lt;0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Serum levels of BAFF, CFB, and MCP-1 in MN patients are associated with anti-PLA2R antibody levels, MN stage, and therapeutic effect. Moreover, the combined detection of serum BAFF, CFB, and MCP-1 has high predictive value for nonremission after t","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1420-1429"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of circulating neuron-specific enolase combined with erythrocyte and platelet distribution indices in patients with severe traumatic brain injury.","authors":"Tengyu Li, Dingjun Ma, Guozhen Zhang, Chao Wang, Bin Xu, Weidong Zhu","doi":"10.5937/jomb0-65680","DOIUrl":"https://doi.org/10.5937/jomb0-65680","url":null,"abstract":"<p><strong>Background: </strong>Severe traumatic brain injury (STBI) is associated with high mortality and long-term neurological disability. Early identification of reliable circulating biomarkers may improve prognostic stratification and clinical management. Haematological distribution indices, including red cell distribution width (RDW) and platelet distribution width (PDW), along with neuron-specific enolase (NSE), reflect systemic inflammation, platelet activation, and neuronal injury following traumatic brain injury. However, their combined prognostic value in STBI remains incompletely understood.</p><p><strong>Methods: </strong>A retrospective study was conducted, including 96 consecutive patients with STBI admitted between March 2020 and March 2023. The GCS score used for rSIG calculation was the first documented admission score before definitive neurosurgical treatment. Treatment was individualised according to injury severity and imaging findings, including conservative management, hematoma evacuation, decompressive craniectomy, ventilatory support, and intensive care when indicated. Peripheral blood levels of RDW, PDW, and serum NSE were measured at admission using automated haematology analysis and enzyme-linked immunosorbent assay. According to the Glasgow Outcome Scale (GOS), evaluated 3 months after injury, patients were classified into a good-prognosis group (n= 46) and a poor-prognosis group (n = 50).</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1243-1250"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum OX40 ligand as a biochemical regulator of Th2-dominant immune activation in asthma: An integrated cytokine network analysis.","authors":"Ziyi Zhu, Shumin Li","doi":"10.5937/jomb0-64381","DOIUrl":"10.5937/jomb0-64381","url":null,"abstract":"<p><strong>Background: </strong>Asthma is characterized by complex immune dysregulation involving coordinated cytokine and chemokine networks. OX40 ligand (OX40L), a key T-cell co-stimulatory molecule, has been implicated in Th2-mediated inflammation; however, its serum-level biochemical interactions with immune activation pathways in asthma remain incompletely defined.</p><p><strong>Methods: </strong>In this cross-sectional study, serum OX40L and immune activation-related factors were quantified in 82 patients with asthma and 40 healthy controls. Multiplex cytokine analysis was performed to measure Th2-, Th17-, proand anti-inflammatory mediators, along with chemokines and total IgE. Lung function indices were assessed by spirometry. Correlation analysis and multivariable linear regression were applied to identify immune factors independently associated with serum OX40L levels.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1295-1306"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of serum CCL3, TRACP-5b, and sclerostin with the prognosis of multiple myeloma patients.","authors":"Cuimei Jiang, Manman Sun, Dashan Gao, Xiaoli Zhang, Jianan Pan","doi":"10.5937/jomb0-62533","DOIUrl":"10.5937/jomb0-62533","url":null,"abstract":"<p><strong>Background: </strong>To investigate the clinical use of Sclerostin, tartrate-resistant acid phosphatase 5b (TRACP-5b), and serum C-C motif chemokine ligand 3 (CCL3) levels in assessing the health and prognosis of patients with multiple myeloma.</p><p><strong>Methods: </strong>A total of 250 patients with multiple myeloma who visited the hospital between January 2023 and December 2024 were included as the observation group, while 150 healthy individuals evaluated during the same period served as the control group. Serum levels of CCL3, TRACP-5b, and Sclerostin were measured and compared in the observation group before and after treatment. Additionally, serum levels of these markers were analysed in MM patients with different tumour stages and varying degrees of bone destruction. Univariate and multivariate analyses were performed to identify factors associated with death within one year in MM patients. Finally, the predictive efficacy of single and combined detection of serum CCL3, TRACP-5b, and Sclerostin for one-year mortality in multiple myeloma patients was evaluated.</p><p><strong>Results: </strong>The levels of serum CCL3, TRACP-5b, and Sclerostin in the observation group before and after treatment were significantly greater than those in the control group, and the differences were statistically significant (P&lt;0.05). After treatment, the levels of serum CCL3, TRACP-5b, and Sclerostin in the observation group were significantly lower than those before treatment, and the differences were statistically significant (P&lt; 0.05). There were statistically significant differences in tumour stage, classification of bone destruction, CCL3 level, TRACP-5b level, and Sclerostin level (P&lt;0.05). Multivariate analysis revealed that tumour stage, the degree of bone destruction, the CCL3 level, the TRACP-5b level, and the Sclerostin level were factors influencing death within 1 year in patients with multiple myeloma (P&lt;0.05). The levels of serum CCL3, TRACP-5b, and Sclerostin have relatively high predictive value for death within 1 year in patients with multiple myeloma. The sensitivity of the combined detection of the three indicators was 90.3%, the specificity was 87.4%, and the area under the receiver operating characteristic curve (AUC) was 0.937, which was significantly greater than that of CCL3 (Z = 3 .0 6 1 , P=0.002), TRACP-5b (Z= 3.625, P&lt;0.001), and Sclerostin (Z= 2.579). When tested separately (P= 0.010), there was no statistically significant difference in AUC among the three indicators (P&gt;0.05).</p><p><strong>Conclusions: </strong>CCL3, TRACP-5b, and Sclerostin are involved in the development and progression of multiple myeloma and have high predictive value for death within 1 year.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1286-1294"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic significance of circulating immune-inflammatory biomarkers IL-6 and systemic immune-inflammation index in hepatocellular carcinoma treated with immune checkpoint inhibitors: A meta-analysis.","authors":"Enze Hu, Yuanfu Qiu","doi":"10.5937/jomb0-64710","DOIUrl":"https://doi.org/10.5937/jomb0-64710","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is characterized by a chronic inflammatory and immunologically dysregulated microenvironment. Circulating immune-inflammatory biomarkers reflect systemic biochemical and immune alterations and may provide laboratory-accessible indicators for prognosis assessment. However, the prognostic value of interleukin-6 (IL-6) and the systemic immune-inflammation index (SII) in HCC patients receiving immune checkpoint inhibitors (ICIs) remains incompletely defined.</p><p><strong>Methods: </strong>A comprehensive literature search was conducted across PubMed, Embase, Web of Science, the Cochrane Library, CNKI, and Wanfang Data to identify cohort studies evaluating circulating IL-6 and/or SII in HCC patients treated with ICIs. Hazard ratios (HR) with 95% confidence intervals (CI) for progression-free survival (PFS) and overall survival (OS) were pooled using fixedor random-effects models. Subgroup and sensitivity analyses were performed to explore sources of heterogeneity.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 6","pages":"1222-1231"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13600549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}