Journal of Medical Biochemistry最新文献

{"title":"Association between vitamin D receptor gene polymorphisms, inflammatory cytokines (IL-1, IL-6, and IL-10), and fracture healing in sports-related injuries.","authors":"Dan Wang","doi":"10.5937/jomb0-56860","DOIUrl":"10.5937/jomb0-56860","url":null,"abstract":"<p><strong>Background: </strong>In this study, we analysed the correlation between polymorphisms in the Vitamin D receptors (VDR) gene at the Apa I and FoK I loci and sports-related fractures (SRF). We also examined the inflammatory cytokines IL-1, IL-6, and IL-10 to explore their potential role in fracture healing.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 110 patients with SRF admitted to our hospital from February 2023 to October 2023. Among these, 41 patients with poor fracture healing (research group) and 69 patients with good fracture healing (control group) were included. Serum levels of Vitamin D (VD), bone mineral density (BMD), and inflammatory cytokines (IL-1, IL-6, and IL-10) were measured to assess their relationship with fracture healing. Additionally, polymorphisms in the VDR gene at the Apa I and FoK I loci were analysed to determine the differences between the two groups.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1357-1365"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum value of fasting C-peptide (FC-P), fasting insulin (FIns), and glycated hemoglobin (HbA1c) after dynamic blood glucose monitoring-guided personalised nutrition and insulin pump therapy for type II diabetes mellitus.","authors":"Qing Zhou, Li Zou, Yan Gao, Hua Ma, Yaping Guo, Guohong Zhu","doi":"10.5937/jomb0-55738","DOIUrl":"10.5937/jomb0-55738","url":null,"abstract":"<p><strong>Background: </strong>Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterised by impaired glucose metabolism, which necessitates comprehensive management of blood glucose (BG), blood pressure, and lipid profiles. This study aimed to evaluate the clinical effects of individualised nutrition and insulin pump therapy, guided by continuous glucose monitoring (CGM) and the Quality Circle Control (QCC) nursing model, on various biomarkers in T2DM patients, including fasting C-peptide (FC-P), fasting plasma glucose (FPG), 2-hour postprandial glucose (2hPG), fasting insulin (FIns), and glycated haemoglobin (HbA1c).</p><p><strong>Methods: </strong>Eighty T2DM patients treated at our hospital were enrolled in the study between January 2023 and January 2024. Patients were assigned to either the experimental group (EG), which received individualised nutrition and insulin pump therapy supported by CGM and the QCC nursing model, or the regular group (RG), which received standard care. Differences in BG control, insulin usage, CGM system performance (including downtime and anomaly rates), and patient satisfaction were compared between the two groups.</p><p><strong>Results: </strong>The EG demonstrated significant improvements in FC-P, FPG, 2hPG, FIns, and HbA1c levels compared to the RG (P<0.05). Specifically, the EG showed more rapid achievement of BG targets, reduced glucose variability, lower insulin usage, and decreased CGM system anomalies.</p><p><strong>Conclusions: </strong>The QCC nursing model, when integrated with individualised nutrition and insulin pump therapy guided by CGM, significantly enhances blood glucose control, optimises insulin therapy, and improves patient outcomes, including dietary habits, quality of life, and reduction in hypoglycemic events. This model shows promise as an effective strategy for managing T2DM and warrants further adoption in clinical practice.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1288-1296"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), IL-6, TNF-a, and IL-1b in preserving kidney function in diabetic renal cell carcinoma patients undergoing partial resection: A focus on the TGF-b1/Smad pathway.","authors":"Pengcheng Ye, Jia Fu","doi":"10.5937/jomb0-55290","DOIUrl":"10.5937/jomb0-55290","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the protective effects of dexmedetomidine (DEX) on renal damage in diabetic patients with renal cell carcinoma undergoing partial nephrectomy. Specifically, it focused on oxidative stress markers, inflammatory cytokines, and the TGF-b1/Smad signalling pathway.</p><p><strong>Methods: </strong>Between August 2022 and July 2024, 100 diabetic patients with renal cell carcinoma undergoing partial nephrectomy were randomly assigned to receive either 1.0 mg/kg of DEX or normal saline (control) during surgery. Blood samples were taken preoperatively, intraoperatively, and postoperatively to measure oxidative stress markers (malondialdehyde [MDA], superoxide dismutase [SOD], glutathione peroxidase [GSH-Px]), inflammatory cytokines (IL-6, TNF-a, IL-1b), and renal function indicators (BUN, Scr, CysC). The TGF-b1/Smad pathway and renal cell apoptosis were also assessed.</p><p><strong>Results: </strong>No significant differences in baseline markers were observed between the groups. However, during and after surgery, the DEX group exhibited significantly lower MDA levels and higher SOD and GSH-Px levels (P<0.05), indicating reduced oxidative stress. Inflammatory markers (IL-6, TNF-a, IL-1b) were also lower in the DEX group.</p><p><strong>Conclusions: </strong>DEX significantly mitigates renal damage in diabetic patients undergoing partial nephrectomy by reducing oxidative stress, inflammation, and apoptosis and by inhibiting the TGF-b1/Smad pathway. These findings suggest its potential as a protective agent for high-risk surgical patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1322-1330"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum ionised calcium, thrombin time (TT), fibrinogen (FIB) after heparin and sodium citrate anticoagulation in children with sepsis.","authors":"Huixia Wang, Hongfang Zhao","doi":"10.5937/jomb0-56286","DOIUrl":"10.5937/jomb0-56286","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to compare the anticoagulant efficacy and bleeding risk of systemic heparin versus local sodium citrate anticoagulation in continuous renal replacement therapy (CRRT) for children with sepsis in the intensive care unit (ICU).</p><p><strong>Methods: </strong>Clinical data from 96 children with sepsis admitted to the ICU of Northwest Women's and Children's Hospital between January 2021 and January 2024 were retrospectively analysed. The children were divided into a control group (CG) and an observation group (OG). Coagulation parameters, the effectiveness of blood purification treatment, and the incidence of bleeding events during CRRT were compared between the two groups.</p><p><strong>Results: </strong>After treatment, significant increases were observed in activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT), while fibrinogen (FIB) levels decreased. APTT, PT, and TT showed more significant increases in the OG, and FIB levels were notably reduced. pH, HCO3-, and Na+ levels were significantly different after treatment, and creatinine (Cr) and urea nitrogen (BUN) levels were lower in both groups, with the OG showing a slightly more significant decrease in Cr and BUN compared to the CG. Ionised calcium (iCa2+) levels were higher in the OG than in the CG. The bleeding incidence in the OG was 27.08%, lower than the 35.42% observed in the CG (all P<0.05).</p><p><strong>Conclusions: </strong>Local sodium citrate anticoagulation demonstrated a favourable anticoagulation effect with a lower bleeding risk than systemic heparin anticoagulation in CRRT for children with sepsis in the ICU.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1241-1250"},"PeriodicalIF":1.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The serum levels of IL-8, IL-10, and TNF-a, cardiac troponin I, creatine kinase-MB in patients with rheumatic heart disease received dexmedetomidine.","authors":"Dongmei Yang, Jian Zhang, Caiyun Zhang","doi":"10.5937/jomb0-56030","DOIUrl":"10.5937/jomb0-56030","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the effects of dexmedetomidine (DEX) in patients undergoing valve replacement surgery for rheumatic heart disease (RHD), focusing on its impact on serum levels of inflammatory markers (IL-8, IL-10, TNF-a) and myocardial injury markers (Cardiac Troponin I [cTnI], Creatine Kinase-MB [CK-MB]).</p><p><strong>Methods: </strong>A total of 140 patients undergoing cardiopulmonary bypass (CPB) valve replacement surgery at West China Hospital, Sichuan University, between January 2022 and December 2024 were randomly assigned to two groups: the observation group (DEX) and the control group (normal saline). Key perioperative parameters were analysed, including anaesthetic dosage, myocardial injury markers, immune function (CD4+, CD8+, and CD4+/CD8+ ratio), inflammatory factors, and adverse reactions.</p><p><strong>Results: </strong>The observation group required significantly lower anaesthetic dosages than the control group. Additionally, the observation group exhibited higher heart rate (HR) at T5 and higher mean arterial pressure (MAP) at T2, T3, and T5 (P< 0.05). Myocardial injury markers (cTnI and CK-MB) were significantly lower in the observation group (P< 0.05). While preoperative cellular immune function (CD4+, CD8+, and CD4+/CD8+) was similar between both groups, postoperative measurements showed significantly higher CD4+ and CD4+/CD8+ ratios, and lower CD8+ in the observation group (P < 0.05). Regarding inflammatory markers, IL-8 and TNF-a levels were significantly lower, while IL-10 was higher in the observation group postoperatively (P < 0 .0 5). There were no significant differences in adverse reactions between the two groups (P > 0 .0 5).</p><p><strong>Conclusions: </strong>Dexmedetomidine (DEX) reduces anaesthetic usage, supports hemodynamic stability, mitigates myocardial injury, and lowers postoperative inflammatory responses in patients undergoing RHD valve replacement surgery.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1028-1037"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive value of serum glutathione S-transferase (GST-â), P-glycoprotein (PGP), P53, KI-67 in breast cancer: A systematic review and meta-analysis.","authors":"Nuan Zhang, Zhipeng Wang, Yang Zhang","doi":"10.5937/jomb0-56147","DOIUrl":"10.5937/jomb0-56147","url":null,"abstract":"<p><strong>Background: </strong>To assess the predictive value of drug-resistant proteins - serum glutathione S-transferase (GST-â), P-glycoprotein (PGP), P53, Ki-67 - in triple-negative breast cancer (TNBC) and their role in chemotherapy resistance. This systematic review and meta-analysis aimed to explore their clinical relevance for improving TNBC treatment outcomes.</p><p><strong>Methods: </strong>We systematically searched PubMed, Web of Science, CNKI, WanFang, and VIP databases for studies from 2010 to 2024. Studies meeting predefined inclusion and exclusion criteria were selected. Data extraction and quality assessment were performed by two independent researchers. Meta-analysis was conducted using RevMan 5.3 software.</p><p><strong>Results: </strong>Seven studies were included, involving 1,772 patients, with 745 TNBC cases and 1,027 non-TNBC cases. Meta-analysis showed that in TNBC compared to non-TNBC, the expression rates of GST-â [O R= 3.41, 95% CI (2.21, 5.25), P< 0.00001], PGP [O R= 1.87, 95% CI (1.17, 2.98), P= 0.008], P53 [O R= 3.65, 95% CI (2.25, 5.91), P< 0.00001], and Ki-67 [O R= 1.19, 95% CI (0.54, 1.84), P= 0.0004] were significantly elevated, indicating higher drug resistance. However, no significant differences were found in Topo I, II, or III expression. Additionally, TNBC patients had poorer disease-free survival (DFS) [O R = 0.30, 95% CI (0.15, 0.59), P=0.0005] and overall survival (OS) [O R=0.17, 95% CI (0.11, 0.28), P<0.00001] compared to non-TNBC patients.</p><p><strong>Conclusions: </strong>The elevated expression of drug-resistant proteins GST-â, PGP P53, and Ki-67 in TNBC suggests that these biomarkers are closely associated with chemotherapy resistance. Monitoring their levels during treatment may help guide more effective clinical strategies for managing TNBC. The findings emphasise the need for personalised therapeutic approaches based on protein expression profiles to improve clinical outcomes for TNBC patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"935-944"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postoperative serum changes in calcium, phosphorus, iPTH, CRP, IL-6, and TNF-p levels in patients with secondary hyperparathyroidism.","authors":"Wenjing Feng, Runtao Xu, Jinghui Mu, Li Jie, Mingjing Cheng","doi":"10.5937/jomb0-55372","DOIUrl":"10.5937/jomb0-55372","url":null,"abstract":"<p><strong>Background: </strong>This study investigates the impact of food structure intervention based on the fast-track surgery (FTS) concept on the postoperative nutritional status, immune response, and recovery outcomes of patients with secondary hyperparathyroidism (SHPT). Specifically, it explores changes in serum calcium and phosphorus levels, immune-reactive parathyroid hormone (iPTH), inflammatory markers (CRf) IL-6, TNF-P), and rehabilitation outcomes following surgical intervention.</p><p><strong>Methods: </strong>Fifty SHPT patients who underwent surgery at the Third Hospital of Hebei Medical University were randomly divided into two groups: a control group (CG) receiving conventional nursing care and an observation group (OG) receiving food structure intervention based on the FTS concept. Preand postoperative comparisons were made for nutritional indicators (hemoglobin, albumin), biochemical markers (calcium, phosphorus, calcium-phosphorus product, iPTH), inflammatory markers (CRF) IL-6, TNF-P), pain levels (Visual Analog Scale), muscle strength (MRC scale), and postoperative complications.</p><p><strong>Results: </strong>The OG showed significantly improved nutritional status, with higher hemoglobin and albumin levels compared to the CG. Additionally, blood calcium levels and calcium-phosphorus product were significantly elevated, while blood phosphorus and iPTH levels were reduced in the OG. Inflammatory markers (CRf) IL-6, TNF-P) were significantly lower in the OG. Pain scores (VAS) were lower, muscle strength (MRC) was higher, and the incidence of complications was significantly reduced in the OG compared to the CG.</p><p><strong>Conclusions: </strong>Food structure intervention based on the FTS concept enhances nutritional status, improves mineral metabolism, reduces postoperative inflammation, and accelerates recovery in SHPT patients. This study supports the implementation of FTS principles in perioperative care to improve outcomes and reduce complications for SHPT patients, offering valuable insights for optimising clinical management and nursing practices in this patient population.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1083-1092"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possibilities and limitations of digital microscopy of blood smear of the modern hematological analyser Sysmex XN-3100 in leukocyte differentiation.","authors":"Nermina Klapuh-Bukvić, Zehra Kurtanović, Damir Šeper","doi":"10.5937/jomb0-55966","DOIUrl":"10.5937/jomb0-55966","url":null,"abstract":"<p><strong>Background: </strong>Differentiation of leukocytes is one of the key diagnostic procedures in clinical medicine, and correct identification of them in a blood smear is of essential importance. Light microscopy is the reference method for leukocyte differentiation; however, it is time-consuming and must be performed by a highly qualified specialist. For this reason, automatic analysers capable of precise and accurate differentiation of blood cells in the examined sample are increasingly present in haematology laboratories. This paper aims to evaluate the performance of the Sysmex XN-3100 analyser, manufactured by SYSMEX CORPORATION, Kobe, Japan., with a focus on the advantages and disadvantages of its digital microscopy in the differentiation of leukocytes and give brief guidelines on the possibilities and limitations of everyday work on the basis of the obtained results.</p><p><strong>Methods: </strong>Digital optical microscopy on 253 samples was performed with primary data (preclassification) collected after the completion of the autoanalysis. Before validating the obtained results, the data were reviewed by a medical biochemistry specialist who confirmed or corrected them. This generated secondary data (reclassification). The two groups of data were statistically analysed using Passing-Bablok regression analysis, Bland-Altman analysis and Spearman correlation.</p><p><strong>Results: </strong>The obtained results showed strong correlations between the primary and secondary analysis in all cells (highest in lymphocyte group (r=0.986), lowest in eosinophil group (r=0.870)) except immature granulocytes and blasts (significant deviation from linearity, p<0.01).</p><p><strong>Conclusions: </strong>The haematology analyser Sysmex XN-3100 shows high performance in leukocyte analysis and differentiation using digital microscopy, but samples containing blasts and immature granulocytes must additionally be analysed by light microscopy.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1009-1019"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of statins on serum sCD40, sCD146 and PAPP-An in patients with coronary heart disease.","authors":"Huawen Li, Xia Zhou, Wenyan Zhu, Jing Zhang","doi":"10.5937/jomb0-55833","DOIUrl":"10.5937/jomb0-55833","url":null,"abstract":"<p><strong>Background: </strong>To analyse the effects of statins on the levels of sCD40, sCD146 and PAPP-An in patients with coronary heart disease (CHD).</p><p><strong>Methods: </strong>126 patients with CHD treated from September 2022 to September 2024 were divided into a study group (n = 84) and a control group (n=42). The study group was randomly divided into groups A (n=42) and B (n=42). Patients in the control group were routinely treated with vasodilator, furosemide, digitalis and other cardiotonic agents. Based on routine treatment, patients in the study group were treated with Atto vastatin calcium tablets in group An and rosuvastatin calcium tablets in group B. Both groups were treated with 20 mg, oral administration before bedtime, and the treatment cycle was 3 months. The cardiac function grade, blood lipid level, serum sCD40, sCD146 and PAPP-A levels and adverse reactions were compared before and after treatment.</p><p><strong>Results: </strong>After treatment, the cardiac function of the three groups improved compared with that before treatment. The effect of patients in groups A and B was better than the control group. The blood lipid levels of the three groups were improved after treatment. The blood lipid levels of groups A and B were better than in the control group. The serum sCD40, sCD146, and PAPP-A levels between groups A and B were no different; after treatment, these in groups A and B were better than those in the control group. After treatment, there were some adverse reactions in all the three groups.</p><p><strong>Conclusions: </strong>Statins effectively treat sCD40, sCD146, and PAPP-An in patients with CHD. They can significantly improve their cardiac function and blood lipid levels and effectively regulate sCD40, sCD146, and PAPP-An levels in patients with coronary heart disease.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1003-1008"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical efficacy and prognosis study of recurrent laryngeal nerve anatomical surgery on serum TNF-a, CRP, interleukins IL-6, IL-10, and IL-1b and outcomes.","authors":"Yongtao Luo, Hui Cheng","doi":"10.5937/jomb0-56808","DOIUrl":"10.5937/jomb0-56808","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the effects of recurrent laryngeal nerve anatomical exposure during thyroidectomy on serum TNF-a, CRP interleukins IL-6, IL-10, and IL-1b, treatment outcomes, complications, and patient prognosis, with a specific focus on inflammatory and stress markers, including interleukins.</p><p><strong>Methods: </strong>110 patients with thyroid lesions undergoing thyroidectomy were randomly assigned to two groups: Expose (n = 55), where the recurrent laryngeal nerve was exposed during surgery, and non-expose (n = 55), where it was not. Various outcome measures were assessed, including surgical efficacy, vocal cord function, serum inflammatory and stress markers (TNF-a, CRP interleukins IL-6, IL-10, and IL-1b), thyroid function changes, postoperative complications (including RLN I), and patient satisfaction. Additionally, quality of life (QoL) was evaluated.</p><p><strong>Results: </strong>The Expose group exhibited a smaller flap area and shorter hospital stays than the non-expose group, though the surgery took longer (P< 0.05). On postoperative day 30, patients in the Expose group showed lower values of fundamental frequency, Jitter, and Shimmer in vocal cords, indicating improved vocal function (P < 0 .0 5). Furthermore, the Expose group had significantly higher QoL scores and a lower incidence of RLNI (3.64% vs. 20.00% , P< 0.05), with improved surgical satisfaction (96.36% vs. 76.36% , P< 0.05). Notably, the Expose group exhibited reduced inflammatory and stress markers levels, including lower TNF-a, CRP IL-6, and IL-1b, and higher IL-10, which correlated with reduced postoperative pain and inflammation.</p><p><strong>Conclusions: </strong>Anatomical exposure of the recurrent laryngeal nerve during thyroidectomy enhances postoperative recovery, reduces the incidence of RLNI, and improves both vocal and parathyroid function. It also attenuates inflammatory and stress responses, as indicated by changes in serum cytokines, thereby enhancing quality of life and patient satisfaction. This approach offers significant advantages for patients undergoing thyroidectomy for various thyroid disorders.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"965-975"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}