Journal of Medical Biochemistry最新文献

{"title":"Correlation of bone metabolism with serum pain mediators and inflammatory cytokines in knee osteoarthritis and their role in predicting poor rehabilitation outcomes: A prospective cohort study.","authors":"Yan Tao, Nan Cai, Juxia Zhang, Yaoping Zhao, Yan Zhou, Pengfei Liu","doi":"10.5937/jomb0-61488","DOIUrl":"10.5937/jomb0-61488","url":null,"abstract":"<p><strong>Background: </strong>The present study was designed to investigate how bone metabolism markers - specifically Procollagen Type I N-Terminal Propeptide (PINP) and C-Telopeptide of Type I Collagen (CTX) - correlate with serum pain mediators (Prostaglandin E2 [PG E2], Norepinephrine [NE], Substance P [SP]) and inflammatory cytokines (Interleukin-1p [IL-ip], Interleukin-6 [IL-6], Tumor Necrosis Factor-a [TNF-a]) in individuals with knee osteoarthritis (KOA). A further aim was to develop a multi-biomarker predictive model for identifying patients at risk of suboptimal rehabilitation outcomes to guide targeted clinical management.</p><p><strong>Methods: </strong>In this prospective cohort study, 184 KOA patients and an 180 healthy controls were enrolled between January 2023 and May 2024. Using baseline serum, biomarker levels were assessed; Enzyme-Linked Immunosorbent Assay (ELISA) was employed for all analytes except SP, which was determined by radioimmunoassay. The primary endpoint, suboptimal rehabilitation outcome, was determined as either a &lt;30% enhancement in the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score or a &lt;2-point decrease on the Visual Analogue Scale (VAS). Model construction utilized multivariate logistic regression, with its performance evaluated through receiver operating characteristic (ROC) curve analysis, obtaining the area under the curve (AUC), sensitivity, and specificity.</p><p><strong>Results: </strong>KOA patients exhibited significantly lower serum PINP levels but elevated levels of CTX, PGE2, NE, SP, IL-1p, IL-6, and TNF-a compared to controls (P&lt; 0.05). PINP demonstrated significant inverse correlations with the pain and inflammatory biomarkers, while CTX showed strong positive correlations with them (P&lt; 0.05). The integrative logistic regression model, integrating PINP, CTX, inflammatory cytokines, and pain mediators, demonstrated excellent predictive value for poor outcomes, with an AUC of 0.862, 83.58% sensitivity, and 78.63% specificity (P&lt; 0.05).</p><p><strong>Conclusions: </strong>Dysregulated bone metabolism (characterized by low PINP and high CTX) in KOA is significantly linked to heightened expression of pain-associated and inflammatory markers. A combined panel of these biomarkers serves as an effective tool for predicting individuals likely to experience suboptimal rehabilitation results.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1005-1013"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum sCD40L and galectin-3 in the prognosis prediction of patients with acute ischemic stroke.","authors":"Zhiqin Chen, Quanhua Xiao, Kunqing Luo, Lin Shuping, Fan Zhaoyang, Xianliang Lai","doi":"10.5937/jomb0-61699","DOIUrl":"10.5937/jomb0-61699","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To explore the value of serum soluble CD40ligand (sCD40L) and galectin-3 (galectin-3) for predictingthe therapeutic effect and prognosis of patients with acuteischemic stroke (AIS).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;180 AIS patients admitted to the hospitalbetween March 2023 and March 2025 were selected asresearch participants. All patients received emergencytreatment with tenecteplase (TNK-tPA) combined withXuesaitong. Based on the therapeutic effect, patients weredivided into two groups: an effective group and an ineffective group. Serum sCD40L and galectin-3 levels weremeasured in both groups before treatment and 4 weeksafter, and the levels were compared. After discharge, allpatients were monitored for 3 months, and, based on theirmodified Rankin Scale (mRS) score, those with a goodprognosis and those with a poor prognosis were assignedto two groups. Using multivariate logistic regression, therisk factors associated with poor prognosis and inadequatetreatment in AIS patients were examined. Serum levels ofsCD40L and galectin-3 were analysed using a receiveroperating characteristic (ROC) curve to assess their prognostic value for poor prognosis and ineffective treatment inAIS patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Of the 180 research subjects, 146 were effectivelytreated (effective group), while 34 were ineffective (ineffective group). There were 142 patients with a good prognosisand 38 with a poor prognosis, representing an incidence of21.11%. After 4 weeks of treatment, serum levels ofsCD40L and galectin-3 were lower in the successful group than in the unsuccessful group (P&lt; 0.05). Serum levels ofsCD40L and galectin-3 were higher in the poor-prognosisgroup than in the favourable-prognosis group (P&lt; 0.05).Multivariate logistic regression analysis showed that AISpatients with elevated serum galectin-3 and sCD40L levelswere at increased risk of inadequate treatment and pooroutcomes (P&lt; 0.05). The areas under the curve (AUCs) forserum galectin-3 and sCD40L in predicting treatment failure in AIS patients were 0.665 and 0.691, respectively,according to ROC analysis; the specificities were 70.08%and 77.51% , respectively, and the sensitivities were 62.53%and 62.58%, respectively. The combined prediction modelfor ineffective treatment using serum galectin-3 andsCD40L yielded an AUC of 0.784, with specificities andsensitivities of 65.18% and 81.25%, respectively. For predicting poor prognosis, serum galectin-3 and scd40ldemonstrated AUCs of 0.774 and 0.838, respectively; theirspecificities were 67.58% and 75.36% , respectively, whiletheir sensitivities were 75.06% and 80.04% , respectively.The combined prediction of serum galectin-3 and scd40lfor poor prognosis was less effective, with an AUC of 0.919,a specificity of 60.05%, and a sensitivity of 90.63%.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Both sCD40L and serum galectin-3 levelshave some prognostic value for poor prognosis and ineffective treatment in AIS pati","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1163-1173"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The predictive value of serum OX-LDL and 25-(OH)-D levels for cerebral edema in patients with hypertensive cerebral haemorrhage.","authors":"Chao Xu, Bo Wang, Niu Liu, Lei Bao","doi":"10.5937/jomb0-62057","DOIUrl":"https://doi.org/10.5937/jomb0-62057","url":null,"abstract":"<p><strong>Background: </strong>To analyse the predictive value of serum oxidation-modified low-density lipoprotein (ox-LDL) and 25-hydroxyvitamin D [25-(OH)-D] levels before minimally invasive hematoma evacuation in patients with hypertensive intracerebral haemorrhage (H ICH) for cerebral oedema.</p><p><strong>Methods: </strong>300 patients with HICH in our hospital from August 2022 to August 2024 were selected as research subjects. Patients were divided into a high-ox-LDL group (ox-LDL 50 mg/dL) and a low-ox-LDL group (ox-LDL&lt;50 mg/dL) according to their preoperative ox-LDL levels. The patients were separated into two groups based on the amount of serum 25-(OH)-D: high-level 25-(OH)-D (25-(OH)-D 30 ng/mL) and low-level 25-(OH)-D (25-(OH)-D&lt;30 ng/mL). The incidence of cerebral oedema within 48 hours after surgery was compared among the groups. Following minimally invasive hematoma evacuation in patients with HICH, the predictive value of preoperative serum ox-LDL and 25-(OH)-D levels for cerebral oedema was evaluated using receiver operating characteristic (ROC) curves.</p><p><strong>Results: </strong>The hematoma volume, fibrinogen level, and incidence of cerebral oedema varied significantly among patients with different serum ox-LDL and 25-(OH)-D levels (P&lt; 0.05). The results of point-biserial correlation analysis revealed that the occurrence of cerebral oedema in HICH patients was positively correlated with the serum ox-LDL level (r= 0.455, P&lt; 0.05) and negatively correlated with the serum 25-(OH)-D level (r= -0.534, P&lt; 0.05). Pearson correlation analysis revealed a negative correlation between serum ox-LDL and 25-(OH)-D (r= -0.444, P&lt; 0.05). The areas under the curve (AUCs) for preoperative serum ox-LDL, 25-(OH)-D alone, and combined detection in predicting cerebral oedema following minimally invasive hematoma evacuation in patients with HICH were 0.777, 0.768, and 0.839, respectively, according to ROC analysis. The AUC of combined detection was the largest.</p><p><strong>Conclusions: </strong>Before minimally invasive hematoma evacuation in patients with HICH, the serum ox-LDL level was high, and the 25-(OH)-D level was low. Preoperative serum ox-LDL and 25-(OH)-D levels have high predictive value for cerebral oedema after minimally invasive hematoma evacuation in patients with HICH.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1174-1182"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic and prognostic value of serum lactate dehydrogenase and amylase in severe acute pancreatitis: A clinical biochemistry-based evaluation.","authors":"Jie Zheng, Jin Wang, Yinong Zhou, Tingting Yu","doi":"10.5937/jomb0-63403","DOIUrl":"https://doi.org/10.5937/jomb0-63403","url":null,"abstract":"<p><strong>Background: </strong>Severe acute pancreatitis (SAP) is characterised by extensive acinar-cell injury, metabolic dysregulation, and systemic inflammatory activation. Reliable biochemical markers for early risk stratification remain limited. Lactate dehydrogenase (LDH) and amylase (AMY), routinely measured enzymes reflecting cellular damage and pancreatic exocrine dysfunction, may provide prognostic value. To evaluate the diagnostic performance of serum LDH and AMY in identifying SAP and predicting short-term outcomes.</p><p><strong>Methods: </strong>This retrospective study included 200 patients with acute pancreatitis. Serum LDH and AMY were measured on admission using standardised clinical chemistry assays with internal quality control. Patients were categorised as MAP, MSAP, or SAP, and SAP cases were further stratified into goodand poor-prognosis 2-week groups. Logistic regression and ROC analyses were used to determine the predictive value of LDH and AMY.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1140-1148"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum levels of immunoglobulins, tumour markers, and chemokines in gastric cancer patients treated with SOX regimen versus oxaliplatin monotherapy.","authors":"Ying Huang, Yueming Hu, Fengwei Gu, Yufeng Ni","doi":"10.5937/jomb0-56172","DOIUrl":"https://doi.org/10.5937/jomb0-56172","url":null,"abstract":"<p><strong>Background: </strong>Gastric cancer is a leading cause of cancer-related mortality, emphasising the need for reliable bio-markers for prognosis and treatment monitoring. This study evaluates serum levels of immunoglobulins (IgA, IgG, IgM), cancer markers (CA125, CEA), and other biomarkers (MRP-14, SDF-1, FSP-1, CXCR4) in gastric cancer patients after treatment.</p><p><strong>Methods: </strong>100 gastric cancer patients were randomised into two treatment groups: oxaliplatin plus capecitabine (reference) and the SOX regimen (oxaliplatin plus S-1; observation). Serum biomarker levels were measured before and after treatment using flow cytometry and enzyme immunoassays. Clinical efficacy, immune response, and chemotherapy-related toxicity were analysed.</p><p><strong>Results: </strong>The SOX regimen demonstrated superior clinical efficacy, with higher objective response (76% vs. 62%) and disease control rates (94% vs. 86%) compared to oxaliplatin monotherapy. The SOX group exhibited significantly higher post-treatment levels of IgA, IgG, and IgM and increased CD 3+, CD 4+, and NK cell counts. Tumour marker levels (CA125, CEA, MRP-14, SDF-1, FSP-1, CXCR4) were lower in the SOX group, with fewer chemotherapy-related adverse effects.</p><p><strong>Conclusions: </strong>The SOX regimen enhances immune function, reduces tumour markers, and improves treatment outcomes compared to oxaliplatin monotherapy. Serum bio-markers may be valuable for monitoring therapeutic responses in gastric cancer patients. However, the study's small sample size and retrospective design limit the generalizability of the findings. Further studies are necessary to confirm these results and validate the prognostic value of serum biomarkers.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1053-1062"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149038937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of inflammatory immune correlation of the coagulation spectrum, PT, FBG, FDP, D-D in autoimmune diseases.","authors":"Yinghong Zhong, Xiao He, Xiufeng Geng, Heng Huang, Zhixian Zhang, Xuezhong Ren, Hong Chen","doi":"10.5937/jomb0-60937","DOIUrl":"https://doi.org/10.5937/jomb0-60937","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To investigate the expression of coagulation markers and their correlations with immunological function and inflammation in patients with autoimmune diseases (Sjogren's syndrome and rheumatoid arthritis).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 183 patients were selected for the study: 61 RA patients who made up the RA group were admitted to our hospital between December 2023 and December 2024, 61 pSS patients composed the pSS group, and 61 normal physical examinees who were in the physical examination centre of our hospital during the same period composed the control group. Baseline clinical indicators of patients in each group before treatment were collected, including the plasma prothrombin time (PT), international normalised ratio (INR), thrombin time (TT), plasma fibrinogen (FBG), partial thromboplastin time (APTT), fibrinogen degradation products (FDP), and plasma D-dimer (D-D).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The expression levels of Pt FBG, T T FDP and DD in the RA group, the pSS group and the normal group were significantly different. The expression levels of PT, FBG, FDP and D-D in the RA group were all greater than those in the pSS group and the control group. The expression level of PT in the pSS group was greater than that in the control group. Analysis of the receiver operating characteristic (ROC) curve showed that, in contrast to those in the normal group, the area under the curve (AUC) of PT in the RA group was 0.638, the AUC of FBG was 0.899, the AUC of FDP was 0.866, and the AUC of D-D was 0.919. The AUC of the combined diagnosis of RA by coagulation indicators was greater than that of the individual detection of each indicator. Compared with that in the normal group, the AUC of PT in the pSS group was 0.618 (P = 0.025), and the AUC of T T was 0.645 (P=0.006). The AUC of the combined diagnosis of coagulation indicators for pSS was greater than that of the individual detection of each indicator. Higher D-D in RA patients was significantly associated with higher levels of hs-CRP CCP and RF, while higher FBG was significantly associated with higher levels of hs-CRP ESR, RF and CCP Correlation analysis revealed that in the RA group, PT, INR, FBG, FDP and D-D were positively correlated with CRP and ESR. On the other hand, T T was negatively correlated with CRP and ESR. FBG, FDP and D-D in the pSS group were positively correlated with CRP and ESR. In addition, the coagulation indicators in the RA group were positively correlated with the immune indicators, whereas in the pSS group, they were partially negatively correlated, both of which were significant. In addition, the coagulation indicators in the RA group were positively correlated with the immune indicators, whereas in the pSS group, they were partially negatively correlated, both of which were significant. In patients with pSS, FBG and FDP are positively correlated with hs-CRP and A PTT and ESR are positively correlated with FBG.&lt;/p&gt;&lt;p&gt;&lt;stron","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1023-1034"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Omentin-1 and CS846: Biomarkers for diagnosis and post-TKA functional outcomes in knee osteoarthritis.","authors":"Yingzhao Qi, Zhixin Liu, Fengli Sun, Ran Liu, Jianfeng Deng, Longfei Hao, Jiayu Wang, Juyuan Gu","doi":"10.5937/jomb0-62196","DOIUrl":"10.5937/jomb0-62196","url":null,"abstract":"<p><strong>Background: </strong>To determine the synergistic diagnostic value of serum Omentin-1 and cartilage oligomeric matrix protein epitope CS846 (CS846) in knee osteoarthritis (KOA) and to explore their link to functional recovery following total knee arthroplasty (TKA), thereby contributing to improved early screening and prognostic strategies.</p><p><strong>Methods: </strong>Osmislili smo prospektivnu kohortnu studiju koja je obuhvatila 84 ispitanika (42 sa KOA i 42 zdrave kontrole). Tehnika enzimski povezanog imunosorbentnog testa (ELISA) korisćena je za merenje serumskog omentina-1, CS846 i inflamatornih indeksa (hs-CRP i ESR). ROC krive (Receiver Operating Characteristic) odredile su dijagno-stičku efikasnost, a za kombinovanu dijagnozu je uspostav-ljen logistički regresioni model. Funkcionalni ishodi, mereni WOMAC rezultatima, i status rehabilitacije su pregledani 6 meseci nakon operacije.</p><p><strong>Results: </strong>Analysis revealed reduced Omentin-1 and elevated CS846 in KOA patients versus controls. The diagnostic per-formance (AU C= 0.851) of the biomarker combination surpassed that of individual tests. Patients with KL1-2 grades exhibited elevated Omentin-1 but lower CS846 levels compared to those with KL3-4 grades (P&lt; 0.05). An inverse correlation was observed between Omentin-1 and inflammatory markers (hs-CRP ESR), contrasting with the positive correlation for CS846. Post-treatment, poor recov-ery was associated with more pronounced decreases in Omentin-1 and increases in CS846 (P&lt; 0.05). The COM-bined model effectively predicted suboptimal rehabilitation outcomes (AU C= 0.857).</p><p><strong>Conclusions: </strong>The synergistic use of serum Omentin-1 and CS846 biomarkers boosts early detection capability for KOA while accurately forecasting post-TKA functional out-comes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"998-1004"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated inflammation-immune-matrix remodeling biomarkers for diagnosis and stage stratification of primary laryngeal cancer.","authors":"Kaisheng Lin, Jiongcong Yuan, Ying Wang","doi":"10.5937/jomb0-63714","DOIUrl":"10.5937/jomb0-63714","url":null,"abstract":"<p><strong>Background: </strong>Aryngeal squamous cell carcinoma (LSCC) is characterized by complex interactions among systemic inflammation, immune dysregulation, and extracellular matrix remodeling. Circulating biomarkers reflecting these biological processes may provide valuable tools for disease detection and stratification; however, their integrated diagnostic value in primary LSCC remains insufficiently defined.</p><p><strong>Methods: </strong>We conducted a case-control study including 50 treatment-naïve LSCC patients and 50 healthy controls. Levels of YKL-40, soluble urokinase plasminogen activator receptor (suPAR) and matrix metalloproteinase-9 (MMP-9) in serum were quantified using ELISA. Systemic inflammation was evaluated via the CRP/albumin ratio and neutrophil-to-lymphocyte ratio (NLR). Receiver operating characteristic (ROC) curves assessed the diagnostic performance of individual and combined biomarkers, and associations with tumor stage were analyzed according to the AJCC 8th edition.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1072-1078"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical alterations in inflammation, oxidative stress, and urinary biomarkers associated with renal damage in systemic lupus erythematosus.","authors":"Zhang Rui, Yiyang Yu, Guangxu Liu, Zhongqiu Luan","doi":"10.5937/jomb0-62936","DOIUrl":"10.5937/jomb0-62936","url":null,"abstract":"<p><strong>Background: </strong>Renal damage is a major complication of systemic lupus erythematosus (SLE) and is closely linked to biochemical disturbances involving inflammation, oxidative stress, and renal tubular injury. However, the biochemical signatures that differentiate SLE patients with and without renal involvement remain insufficiently characterized. To evaluate the biochemical profiles of inflammatory cytokines, oxidative stress markers, and urinary renal-injury biomarkers in SLE patients with and without renal damage, and to explore their associations with microbial alterations.</p><p><strong>Methods: </strong>Sixty-four SLE patients were classified into a renal damage group (n = 36; positive urine protein) and an SLE-only group (n = 28; negative urine protein). Serum cytokines (TGF-R, IL-4, IL-17, IL-1P) and oxidative stress indicators (SOD, T-AOC, MDA) were quantified using ELISA. Urinary microprotein, microglobulin, and N-acetyl-P-D-glucosaminidase (NAG) were used as biochemical markers of renal injury. Oral and intestinal microbial profiles were analyzed by sequencing, and correlations between biochemical markers and microbial alterations were assessed.</p><p><strong>Results: </strong>SLE patients with renal damage showed significantly elevated urinary microprotein, microglobulin, and NAG (all p &lt; 0.001). Inflammatory cytokines were markedly increased in the renal damage group (TGF-P, IL-4, IL-17, IL-1P; all p &lt; 0.05), whereas oxidative stress capacity was significantly reduced (SOD, T-AOC, MDA; all p &lt; 0.05). Several microbial taxa correlated positively or negatively with key biochemical markers, suggesting potential metabolic-immune interactions contributing to renal injury.</p><p><strong>Conclusions: </strong>Renal damage in SLE is characterized by distinct biochemical abnormalities involving intensified inflammation, impaired antioxidant defenses, and elevated urinary renal-injury biomarkers. These biochemical changes, together with specific microbial shifts, may contribute to the progression of SLE-related renal impairment and hold diagnostic value for early biochemical screening.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1113-1119"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiometabolic parameters and novel hematological indices in women with migraine.","authors":"Aleksandra Klisic, Ana Tmusic","doi":"10.5937/jomb0-62189","DOIUrl":"10.5937/jomb0-62189","url":null,"abstract":"<p><strong>Background: </strong>The pathophysiological aspect of migraine has not been fully elucidated. It is assumed that inflammation is at the root of this disease. The goal of the research is to examine cardiometabolic and novel haematological indices in patients with migraine.</p><p><strong>Methods: </strong>A total of 50 women diagnosed with migraine were included in the study. Women were recruited during pain-free periods between pain attacks. The control group consisted of 25 age-matched healthy women. The MIDAS score (Migraine Disability Assessment score) was calculated.</p><p><strong>Results: </strong>Women with migraine had higher levels of uric acid (P = 0.045) and higher transaminase activity, AST (P&lt; 0.001) and ALT (P= 0.003). Significantly lower neutrophil counts and RDW-CV (%) and higher platelet count (PLT) and PCT were observed in subjects with migraine. The derived haematological parameters, i.e., NLR, dNLR, M-GLR, and PDW/PCT, were significantly lower in the migraine group than in the controls, whereas higher PNR was observed in the migraine group. Among the examined haematological indicators, PNR showed excellent diagnostic accuracy (AUC= 0.727), while other parameters (RDW, PLT, neutrophils, PDW/PCT, NLR, dNLR, M-GLR) showed good diagnostic accuracy (0.600&lt; AUC&lt; 0.700). The largest number of women suffering from migraine (52.9%) had a MIDAS score of 11-20. Creatinine showed good diagnostic accuracy in predicting high MIDAS scores (AUC= 0.678).</p><p><strong>Conclusions: </strong>Women suffering from migraine have less favourable cardiometabolic status in comparison to healthy women. Blood count parameters and calculated indices have good diagnostic accuracy for migraine. These parameters are cost-effective and easily available.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1120-1130"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}