Journal of Medical Biochemistry最新文献

{"title":"Changes in serum interleukin-6 (IL-6) and C-reactive protein (CRP), PCT after early resuscitation in patients with severe acute pancreatitis.","authors":"Jinlong Wang, Zhanghua Guo, Xin Chai, Dupeng Li, Binxiao Su, Peng Jiang","doi":"10.5937/jomb0-55659","DOIUrl":"10.5937/jomb0-55659","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the effects of different early resuscitation fluid replenishment rates (FRRs) on inflammation (serum interleukin-6 (IL-6) and C-reactive protein (CRP), PCT and complications in patients with severe acute pancreatitis (SAP).</p><p><strong>Methods: </strong>Sixty-six patients with SAP were studied. According to the ratio of total fluid replenishment 24 h after admission to 72 h (FRR), the patients were rolled into a low FRR group (Low group), a moderate FRR group (Moderate group), and a high FRR group (High group), with 22 cases in each. Serum-related indexes, APACHE II score, HCT, systemic inflammatory response syndrome (SIRS) duration, length of hospital stay (LOS), and complication rate (CR) were determined and compared.</p><p><strong>Results: </strong>The results suggested that ALT, AST, SCr, BUN, TBil, APACHE II, scores and HCT in the Moderate group were the lowest (P< 0.05), while those in the High group were the highest (P<0.05). After the patients were treated for 72 h, the IL-6, CRP and PCT in the Low and High groups were higher than those in the Moderate groups, exhibiting differences with P<0.05 and P<0.01, respectively. The SIRS duration and LOS in the Low and High groups were longer. They presented differences with P<0.05 and P<0.01 to the Moderate group, respectively. The rates of MODS, mechanical ventilation, pancreatic necrosis infection, and death in the Moderate group were the lowest (P<0.05).</p><p><strong>Conclusions: </strong>the moderate FRR could effectively alleviate the inflammatory response of patients with SAP shorten the treatment time, and reduce the CR.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1050-1058"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Esketamine modulates postoperative biochemical markers of oxidative stress, inflammation, and immune dysregulation in laparoscopic colorectal cancer surgery.","authors":"Peng Chen, Kun Qian, Kairun Zhu, Lu Xiaoqing, Liu Dajin","doi":"10.5937/jomb0-56862","DOIUrl":"10.5937/jomb0-56862","url":null,"abstract":"<p><strong>Background: </strong>Laparoscopic colorectal cancer surgery, while minimally invasive, induces systemic oxidative stress, inflammation, and immune dysfunction through surgical trauma and anesthesia-related stress. Esketamine, an NMDA receptor antagonist with antioxidant and anti-inflammatory properties, may mitigate these biochemical perturbations. This study evaluated esketamine's effects on serum biomarkers of oxidative stress (glutathione, catalase, malondialdehyde, superoxide dismutase), inflammatory mediators (TNF-a, CRP IL-6), and T lymphocyte subsets in patients undergoing laparoscopic colorectal cancer resection.</p><p><strong>Methods: </strong>In this randomized controlled trial, 150 stage I-II colorectal cancer patients were allocated to esketamine (0.25 mg/kg bolus + 0.12 mg/kg/h infusion) or control (saline) groups during standardized anesthesia. Preand postoperative serum levels of oxidative stress markers (GSH, CAT, MDA, SOD), inflammatory cytokines (TNF-a, CRP IL-6), and immune cell subsets (CD3+, CD4+, CD8+, CD4+/CD8+ ratio) were quantified via ELISA and flow cytometry. Statistical analysis compared intergroup differences using t-tests and chi-square tests.</p><p><strong>Results: </strong>Postoperatively, the esketamine group exhibited significantly attenuated oxidative stress, with higher GSH (72.43± 6.63 vs. 60 .1 6± 5.57 mg/mL, P < 0.05), CAT (92.56± 8.31 vs. 82.81 ± 7.75 U/mL), and SOD (84.53± 8.02 vs. 69 .93± 7.05 nU/mL), alongside lower MDA (6.41± 0.52 vs. 9.52± 0.63 mmol/L). Pro-inflammatory cytokines were reduced (TNF-a: 4 0 .32 ± 4.84 vs. 54.37± 5.80 pg/mL; IL-6: 50.83± 5.05 vs. 82 .38± 8.46 pg/mL, P < 0.05). Immune function preservation was evident through elevated CD3+ (4 5 .1 8 ± 5 .0 1 % vs. 37 .05 ± 4.92% ) and CD4+ T cells (26.51 ±2.76% vs. 19.78± 2.09%), with a balanced CD4+/CD8+ ratio (1.12± 0.12 vs. 0.72± 0.09).</p><p><strong>Conclusions: </strong>Esketamine-based anesthesia significantly ameliorates postoperative oxidative damage, suppresses inflammatory cytokine release, and preserves cellular immune homeostasis, as evidenced by targeted biochemical and immunological analyses. These findings highlight esketamine's role in modulating perioperative biochemical pathways, potentially enhancing recovery in colorectal cancer surgery.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1020-1027"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Baoyuan decoction and Taohong Siwu decoction combined with Western medicine on serum lipid levels in elderly patients with coronary heart disease.","authors":"Bingrui Wu, Shenggao Zhang, Ruihong Zhou","doi":"10.5937/jomb0-52017","DOIUrl":"10.5937/jomb0-52017","url":null,"abstract":"<p><strong>Background: </strong>To explore how Baoyuan and Taohong Siwu decoctions combined with Western medicine affect triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in elderly patients with coronary heart disease.</p><p><strong>Methods: </strong>Ninety elderly patients with coronary heart disease who were hospitalized from January 2021 to December 2022 were selected and divided into two groups based on different treatment methods, with 45 patients in each group. The control group was treated with Western medicine alone, while the research group was treated with the combination of Baoyuan and Taohong Siwu decoctions based on the control group. The improvement of traditional Chinese medicine symptom scores, various blood lipid levels, and serum indicators of cardiac function were compared between two groups before treatment, 1 month, 2 months, and 3 months of treatment.</p><p><strong>Results: </strong>Before treatment, the two groups had identical Traditional Chinese Medicine symptom scores, various blood lipid levels, and various serum indicators of cardiac function (P> 0.05). After treating 1 month, 2 months, and 3 months, the chest pain, tightness, fatigue, and sweating scores in the research group were lower (P< 0.05). The TG, TC, LDL-C, and HDL-C levels in the research group were lower (P< 0.05). The levels of plasma N-terminal brain natriuretic peptide (NT-proBNP) and monocyte chemoattractant protein-1 (MCP-1) in the research group were lower (P <0.05).</p><p><strong>Conclusions: </strong>Combining Baoyuan and Taohong Siwu decoctions and Western medicine treatment can help improve the symptoms and signs of coronary heart disease in the elderly. It can reduce the levels of various indicators such as LDL-C, TG, TC, and HDL-C in the body and promote the improvement of cardiac function.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"985-993"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune function (serum IL-4 and IL-5), nutritional status, and clinical outcomes in children with bronchial asthma after vitamin D supplementation.","authors":"Xiangli Xiao, Ranran Wang, Li Qin","doi":"10.5937/jomb0-56915","DOIUrl":"10.5937/jomb0-56915","url":null,"abstract":"<p><strong>Background: </strong>This study explores the combined therapeutic effects of pediatric massage and vitamin D supplementation on the nutritional status, immune function (serum IL-4 and IL-5), and clinical outcomes in children with Bronchial asthma (BA).</p><p><strong>Methods: </strong>A total of 60 children diagnosed with BA were randomly assigned to one of two groups: a control group receiving conventional treatment alone and an experimental group receiving conventional treatment alongside pediatric massage and daily vitamin D supplementation. Both groups were monitored over two months for changes in clinical symptoms, immune markers (CD 3+, CD4+, CD4+/CD8 + ratio), nutritional protein levels (albumin, transferrin, prealbumin, and total protein), and recurrence rates.</p><p><strong>Results: </strong>The experimental group showed significantly faster symptom relief and improved immune function than the control group. This included enhanced immune markers, such as increased CD3+ and CD4+ counts and an improved CD 4+/CD 8+ ratio. Nutritional status also improved, as evidenced by higher levels of albumin, transferrin, prealbumin, and total protein. Additionally, the recurrence rate in the experimental group was notably lower (10%) compared to the control group. The experimental group also exhibited reduced levels of inflammatory cytokines, including IL-4 and IL-5, suggesting a beneficial effect on immune modulation.</p><p><strong>Conclusions: </strong>The combination of pediatric acupressure and vitamin D supplementation can improve immune function and inflammatory response in children with BA, which is of clinical value.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1059-1066"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value and therapeutic efficacy of serum levels of pro-gastrin-releasing peptide precursor (ProGRP) and neuron-specific enolase (NSE) in patients with lung cancer.","authors":"Ping Wang, Mingjie Yu","doi":"10.5937/jomb0-50852","DOIUrl":"10.5937/jomb0-50852","url":null,"abstract":"<p><strong>Background: </strong>Primary lung cancer is one of the most prevalent malignant tumours in China. Small cell lung cancer (SCLC) is a highly malignant, undifferentiated tumour prone to metastasis and is usually diagnosed in its middle or late stages. Pro-gastrin-releasing peptide precursor (ProGRP) and neuron-specific enolase (NSE) tumour markers are recommended in the literature for early diagnosis. Objective: The purpose of this research is to probe the diagnostic value and therapeutic efficacy of serum levels of ProGRP and NSE in SCLC to enhance the level of clinical diagnosis.</p><p><strong>Methods: </strong>A total of 84 SCLC patients who were admitted to our hospital from December 2022 to March 2024 were included in the SCLC group. The NSCLC group consisted of 45 patients diagnosed with NSCLC, while the benign lung disease group consisted of 57 patients diagnosed with non-cancerous lung conditions. Furthermore, the healthy control group comprised 60 healthy individuals. The serum levels of ProGRP and NSE were compared across all four groups.</p><p><strong>Results: </strong>The SCLC group exhibited considerably elevated serum ProGRP and NSE levels compared to the healthy control group, benign lung disease group, and NSCLC group (P< 0.05). ProGRP and NSE values were higher in limited-stage SCLC than in extensive-stage SCLC (P < 0.05). The ROC curve displayed that the critical value of ProGRP for diagnosing SCLC was 136.49 pg/mL, the area under the curve (AUC) was 0.869, the sensitivity attained 80.00%, and the specificity reached 84.87%, indicating a better diagnostic efficacy than that of NSE (P< 0.05).</p><p><strong>Conclusions: </strong>The tumour markers ProGRP and NSE levels are of paramount significance for the clinical diagnosis and staging of SCLC patients. ProGRP is a more specific and sensitive tumour marker for SCLC than NSE and can be employed as an auxiliary diagnostic tool for SCLC. Thus, it is worth promoting ProGRP in a clinical setting.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"945-954"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The changes of serum inflammatory cytokines in patients with hemiplegia after ischemic stroke and the rehabilitation effects.","authors":"Hongxia Li, Yunqi Lai","doi":"10.5937/jomb0-55968","DOIUrl":"10.5937/jomb0-55968","url":null,"abstract":"<p><strong>Background: </strong>Acute ischemic stroke (AIS) often leads to hemiplegia, significantly impairing neurological function, motor ability, and daily life activities. Early precision exercise rehabilitation has emerged as a promising intervention to enhance recovery. This study evaluated its effectiveness in improving neurological function, gait performance, and self-care ability and reducing inflammatory response in hemiplegic patients.</p><p><strong>Methods: </strong>This retrospective cohort study included 230 patients with hemiplegia due to AIS, admitted within 72 hours of onset. Patients were divided into an Early Rehabilitation Group (EG, n = 132) and a Conventional Rehabilitation Group (CG, n = 98) based on the intervention received. The EG underwent early precision exercise rehabilitation, integrating neurofunctional training, motor-evoked potential (MEP) therapy, and functional electrical stimulation (FES), while the CG received traditional rehabilitation. The effectiveness was assessed using the National Institutes of Health Stroke Scale (NIHSS), Wisconsin Gait Scale (WGS), and Activities of Daily Living (ADL) scores. Serum inflammatory markers (TNF-a, hs-CRP IL-6, IL-18) were also measured before and after treatment.</p><p><strong>Results: </strong>The EG demonstrated significantly more significant improvements in NIHSS (5.85± 1.31 vs 7.03± 2.54, P< 0.05), WGS (24.81± 3.06 vs. 31 .96 ± 4.62 , P< 0.05), and ADL scores (63.08± 4.93 vs. 51 .78 ± 6.34 , P< 0.05) compared to the CG. Walking frequency and speed were also higher in the EG (P< 0.05). Inflammatory markers significantly decreased post-treatment in the EG (TNF-a: P< 0.05, hs-CRP: P< 0.05, IL-6: P< 0.05, IL-18: P< 0.05), suggesting a reduction in systemic inflammation.</p><p><strong>Conclusions: </strong>Early precision exercise rehabilitation significantly enhances neurological function, motor ability, and self-care capacity, reducing inflammatory response in hemiplegic AIS patients. These findings support its integration into clinical stroke rehabilitation protocols.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"994-1002"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioration of T lymphocyte subsets, inflammatory factors and gastrointestinal function by traditional Chinese medicine rehabilitation management in postoperative chemotherapy for lung cancer.","authors":"Xiangni Zou, Yuyang Tang, Pei Shi","doi":"10.5937/jomb0-56918","DOIUrl":"10.5937/jomb0-56918","url":null,"abstract":"<p><strong>Background: </strong>To analyse the influence of Traditional Chinese Medicine (TCM)-characteristic rehabilitation management on T lymphocyte subsets, inflammatory response, and stress response during the postoperative chemotherapy of lung cancer (LC).</p><p><strong>Methods: </strong>189 LC patients admitted to our hospital from July 2023 to June 2024 were enrolled in this research. Among them, 107 patients who did not receive TCM-characteristic rehabilitation management were regarded as the control group, while the other 82 patients who underwent such management were designated as the observation group. The levels of inflammatory factors and stress response markers before and after treatment in both groups were compared, including hypersensitive-C reactive protein (hs-CRP), interleukin-6 (IL-6), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px). The T lymphocyte subsets [CD3+, CD4+, CD8+, and natural killer (NK) cells] and gastrointestinal function were also detected.</p><p><strong>Results: </strong>After the completion of treatment, it was observed that the levels of hs-CRP MDA, and the proportion of CD8+ T lymphocyte subsets in the observation group were significantly reduced compared with the control group (P< 0.05). In contrast, the activities of SOD and GSH-Px, the proportions of CD3+ and CD4+ T lymphocyte subsets, as well as the activity of NK cells in the observation group were notably higher versus the control group (P< 0.05). Moreover, the gastrointestinal function of the patients in the observation group exhibited a more favourable condition after treatment (P< 0.05).</p><p><strong>Conclusions: </strong>TCM-characteristic rehabilitation management can effectively ameliorate inflammatory responses and stress injury in patients undergoing postoperative chemotherapy for LC and enhance their immune and gastrointestinal functions.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"955-964"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differently expressed miRNA in plasma samples of immune thrombocytopenic purpura patients and its clinical significance.","authors":"Yuqian Yao, Hemeng Zhao, Xiaoyu Zhu, Yafei Fang, Yue Feng","doi":"10.5937/jomb0-52498","DOIUrl":"10.5937/jomb0-52498","url":null,"abstract":"<p><strong>Background: </strong>To guide clinical doctors, current work has been designed to explore the abnormal expressions of miRNAs in plasma samples of patients with immune thrombocytopenic purpura (ITP).</p><p><strong>Methods: </strong>Bioinformatic analysis was performed using the GSE80401 chip. The study subjects were recruited from the First People's Hospital of Lianyungang between May 2021 and December 2023. 48 ITP patients admitted to the intensive care unit were enrolled. miRNA levels were examined using real-time polymerase chain reaction. All data were analysed using SPSS 22.0 software. The potential diagnosis value of the significantly up-regulated and down-regulated miRNAs was evaluated using a receiver operator characteristic (ROC) curve.</p><p><strong>Results: </strong>We performed bioinformatical analysis on the GSE80401 chip and identified the differently expressed miRNAs in ITP patients compared to the controls, and the results of the heat map showed the results. GO and pathway analysis revealed the process that involved the differently expressed miRNAs. Next, the results of RT-qPCR analysis showed the levels of miR-877-3p, miR-425-3p, miR-122-5p, miR-1281, and miR-1825 were significantly increased. In contrast, the miR-3945, miR-4430, miR-3158-5p, miR-3131, and miR-4655-3p levels were markedly decreased in plasma samples of ITP patients compared with the controls. Finally, results showed that the area under the ROC curve of miRNAs was as follows: miR-877-3p, 0.9349, miR-425-3p, 0.8607, miR-1281, 0.7131, miR-1825, 0.8928, miR-3945, 0.8459, miR-4430, 0.8112, miR-3158-5p, 0.6059, miR-3131, 0.8989, suggesting that the above miRNAs may serve as biomarkers for distinguishing the ITP patients from healthy controls.</p><p><strong>Conclusions: </strong>miRNAs may have predictive value for the diagnosis of ITP. The results of current work may provide new clues for the pathogenesis of ITP, which in turn offers a new theoretical basis and therapeutic tools for the clinical diagnosis and treatment of ITP.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1118-1126"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of inflammatory biomarkers RBP4, Lipocalin-2, and hsCRP in prediabetes and type 2 diabetes mellitus.","authors":"Diker Vesile Ornek, Ozden Serin, Ertug Ebru Yorulmaz, Ozlem Baytekin, Osman Oguz, Güvenç Güvenen","doi":"10.5937/jomb0-57001","DOIUrl":"10.5937/jomb0-57001","url":null,"abstract":"<p><strong>Background: </strong>Adipocytokines, along with macrophages infiltrating adipose tissue, contribute to chronic low-grade inflammation, which leads to insulin resistance and type 2 diabetes. Understanding insulin resistance in non-diabetic individuals and its cellular mechanisms is key for developing effective treatments and improving current protocols. This study aimed to investigate the levels of Retinol Binding Protein 4 (RBP4), Lipocalin-2, and high-sensitivity C-reactive protein (hsCRP) in individuals with impaired fasting glucose (IFG), impaired glucose tolerance (IGT), and type 2 diabetes mellitus (T2DM).</p><p><strong>Methods: </strong>This prospective case-control study included individuals with IFG, IGT, and newly diagnosed T2DM. Routine laboratory tests, including fasting blood glucose, insulin, HbA1c, and lipid profiles, were collected and analysed. RBP4, Lipocalin-2, and hsCRP levels were measured using the ELISA method.</p><p><strong>Results: </strong>Significant differences were found in fasting blood glucose, insulin, triglycerides, total cholesterol, and HOMA-IR values among the study groups. hsCRP levels were significantly elevated in the IGT and T2DM groups compared to controls, while RBP4 and Lipocalin-2 levels showed no significant differences. A positive correlation was observed between hsCRP and HbA1c in the IFG group, as well as between hsCRP and Lipocalin-2 in the T2DM group.</p><p><strong>Conclusions: </strong>This study demonstrates that hsCRP and Lipocalin-2 are associated with early glucose metabolism abnormalities and may serve as markers for insulin resistance and inflammation in prediabetes and T2DM. Future research is needed to clarify the roles of these biomarkers and their potential as therapeutic targets in diabetes prevention and treatment.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1067-1073"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of propofol and sevoflurane on metabolic changes and oxidative stress markers in laparoscopic appendectomy in children: Clinical control trial.","authors":"Galambos Izabella Fabri, Biljana Drašković, Marina Pandurov, Goran Rakić, Nikola Bošković, Sanja Vicković, Dragana Pap, Anna Uram-Benka","doi":"10.5937/jomb0-56035","DOIUrl":"10.5937/jomb0-56035","url":null,"abstract":"<p><strong>Background: </strong>Laparoscopic appendectomy offers several advantages over open surgery, including shorter recovery time and less postoperative pain. However, it also poses some risks due to the creation of pneumoperitoneum. Understanding and mitigating this risk through the use of appropriate anaesthetic agents, antioxidant therapy, and optimised surgical techniques can improve patient outcomes. This study aimed to determine which of the two anaesthetics, propofol or sevoflurane, provides better suppression of oxidative, inflammatory and metabolic responses in children undergoing laparoscopic or conventional appendectomy.</p><p><strong>Methods: </strong>This randomised, clinical control trial enrolled 120 children aged 7 to 17 years with a diagnosis of acute appendicitis. Key parameters, including blood glucose, white blood cells, IL-6, oxidative stress markers, lactate, pH, and mean arterial pressure, were analysed.</p><p><strong>Results: </strong>A statistically significant change in lactate and mean arterial pressure values was observed over time in all four study groups. However, neither the type of surgical procedure nor the type of anaesthesia significantly influenced glucose, white blood cells or thiobarbituric acid levels. The postoperative values of IL-6 were lower in patients treated with balanced anaesthesia.</p><p><strong>Conclusions: </strong>Our study demonstrated that the type of anaesthesia affects hemodynamic changes, which are closely related to the occurrence of reperfusion injury. Although markers of tissue hypoxia and acidosis were elevated during laparoscopic surgery, their values quickly normalised in the immediate postoperative period.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 5","pages":"1110-1117"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}