Journal of Medical Biochemistry最新文献

{"title":"Correlation analysis of serum FGF9, Sestrin2 and HBDH with the severity of sepsis.","authors":"Jian He, Xinru Lin, Xiang Ding, Maoxia Liu","doi":"10.5937/jomb0-61187","DOIUrl":"https://doi.org/10.5937/jomb0-61187","url":null,"abstract":"<p><strong>Background: </strong>To explore the correlation between serum fibroblast growth factor 9(FGF9), Sestrin2 and hydroxybutyrate dehydrogenase (HBDH) and the severity of sepsis, and to analyse the predictive value of FGF9, Sestrin2 and HBDH for clinical outcome.</p><p><strong>Methods: </strong>A retrospective analysis of 125 patients with sepsis admitted to Taikang Xianlin Drum Tower Hospital and Chongqing University Central Hospital between May 2022 and May 2025 was conducted. According to disease severity, there were 50 cases in the septic shock group and 75 in the sepsis group. Based on clinical outcomes, patients were divided into a death group (103 cases) and a survival group (22 cases). Observe the levels of serum FGF9, Sestrin2 and HBDH in each group. Analysis of the relationships among FGF9, Sestrin2, HBDH, and sepsis severity was performed using the Spearman correlation coefficient. Using multivariate logistic regression analysis, the factors influencing the prognosis of patients with sepsis were investigated. FGF9, Sestrin2, and HBDH were evaluated for their prognostic utility in sepsis patients using receiver operating characteristic (ROC) curves.</p><p><strong>Results: </strong>Compared with the sepsis group, the level of FGF9 in the septic shock group was lower [(122.41± 21.45) pg/mL vs (145.36± 23.68) pg/mL], while the levels of Sestrin2 and HBDH were both higher [(15.75± 2.34) ng/mL] vs (8.36± 0.93) ng/mL, (232.14± 34.77) U/L vs (166.25± 24.85) U/L]; the differences were statistically significant (P&lt; 0.05). Compared with the survival group, the level of FGF9 in the death group was lower [(112.05± 20.61) pg/mL vs (133.25± 22.14) pg/mL], while the levels of Sestrin2 and HBDH were both higher [(19.25± 2.85) ng/mL] vs (12.42± 2.33) ng/mL, (261.25± 37.25) U/L vs (204.25± 32.18) U/L]. Spearman correlation coefficient analysis showed that FGF9 was negatively correlated with the severity of sepsis (P&lt; 0.05), whereas the degree of sepsis was positively correlated with both Sestrin2 and HBDH (P&lt; 0.05). Sequential Organ Failure Assessment (SOFA), Acute Physiology and Chronic Health Evaluation II (APACHE II), procalcitonin, FGF9, Sestrin2, and HBDH are risk factors for sepsis prognosis, according to multivariate logistic regression analysis (P&lt; 0.05). The results of ROC curve analysis showed that the combined detection of FGF9, Sestrin2 and HBDH had a better predictive effect on the prognosis of patients with sepsis (P&lt; 0.05).</p><p><strong>Conclusions: </strong>The amount of FGF9 in septic shock patients is comparatively low, while the levels of Sestrin2 and HBDH are relatively high. As the severity of the disease increases, the level of FGF9 gradually decreases, while the levels of Sestrin2 and HBDH gradually increase, with a certain correlation with disease severity. The prognosis of sepsis patients can be assessed by measuring FGF9, Sestrin2, and HBDH levels.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1044-1052"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of serum CHI3L1, HMGB1, and CD62E detection with the prognosis of Mycoplasma pneumoniae in children.","authors":"Hui Yang, Tingli Zhang, Xiaoqi Zhang, Yang Xu, Jiang Xiaoan, Bo Chen","doi":"10.5937/jomb0-62529","DOIUrl":"https://doi.org/10.5937/jomb0-62529","url":null,"abstract":"<p><strong>Background: </strong>To evaluate the clinical value of serum Chitinase-3-like protein 1 (CHI3L1), high mobility group protein 1 (HMGB1), and CD62E detection for determining the severity and prognosis of Mycoplasma pneumoniae in children.</p><p><strong>Methods: </strong>A total of 476 children with Mycoplasma pneumoniae who were diagnosed and treated at this hospital from January 2023 to March 2025 were selected as the Mycoplasma pneumoniae infection group. The healthy control group consisted of 120 children who underwent hospital examinations during the same time period. Serum levels of CHI3L1, HMGB1, and CD62E were compared between the Mycoplasma pneumoniae infection group and the healthy control group. To investigate risk factors for a poor outcome in kids infected with Mycoplasma pneumoniae, univariate and multivariate logistic regression analyses were performed. Serum levels of CHI3L1, HMGB1, and CD62E were investigated in relation to Mycoplasma pneumoniae severity and their predictive power for a poor outcome.</p><p><strong>Results: </strong>HMGB1, CD62E, and CHI3L1 serum levels were significantly greater in the Mycoplasma pneumoniae infection group than in the healthy control group (P&lt; 0.05), and they increased as Mycoplasma pneumoniae infection severity increased (P&lt; 0.05). The proportion of children with Mycoplasma pneumoniae with pleural effusion, the proportion of children with a treatment course of S7 days, the white blood cell count, the duration of antibacterial drug use, and the levels of serum CHI3L1, HMGB1, and CD62E were significantly greater in the poor prognosis group than those in the good prognosis group (P&lt; 0.05). However, no statistically significant differences were seen in CRP levels, age, or sex (P&gt; 0.05). Elevated serum CHI3L1, HMGB1, and CD62E levels were found to be independent risk factors for the prognosis of Mycoplasma pneumoniae by multivariate analysis (P&lt; 0.05). The levels of serum CHI3L1, HMGB1, and CD62E have relatively high predictive value for a poor prognosis in patients with Mycoplasma pneumoniae. The sensitivity of the combined detection of the three was 97.9%, the specificity was 90.2%, and the area under the curve was 0.785, which was significantly greater than that of the individual detection of CHI3L1, HMGB1, and CD62E (Z = 3.257, 3.429, 4.650, P&lt;0.05).</p><p><strong>Conclusions: </strong>The levels of serum CHI3L1, HMGB1, and CD62E are indicators reflecting the severity of Mycoplasma pneumoniae. The combined detection method has high efficacy in predicting the poor prognosis of Mycoplasma pneumoniae patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1183-1191"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MTHFR gene polymorphisms and their biochemical associations with serum vitamin D and IL-17 in ulcerative colitis.","authors":"Guang Chen, Meihong Yan, Xiaoyang Hu, Hanfei Xu, Shengai Piao","doi":"10.5937/jomb0-62831","DOIUrl":"10.5937/jomb0-62831","url":null,"abstract":"<p><strong>Background: </strong>Disturbances in homocysteine metabolism and immune-inflammatory pathways may contribute to the pathogenesis of ulcerative colitis (UC). Methylenetetrahydrofolate reductase (MTHFR) plays a central biochemical role in homocysteine remethylation, and its genetic variants may influence downstream biomarkers such as vitamin D and interleukin-17 (IL-17). This study examined the associations of three MTHFR polymorphisms (rs110298, rs132981, rs167281) with UC susceptibility, disease characteristics, and related biochemical markers.</p><p><strong>Methods: </strong>A total of 124 UC patients and 128 healthy controls were enrolled. Genotyping of MTHFR SNPs was performed by PCR amplification followed by ligase detection reaction. Serum 25-hydroxyvitamin D was measured using electrochemiluminescence immunoassay, and IL-17 levels were quantified by ELISA. Associations between MTHFR genotypes, UC onset, disease location, severity, and biochemical markers were statistically evaluated.</p><p><strong>Results: </strong>All polymorphisms conformed to Hardy-Weinberg equilibrium. The rs110298 GG genotype and rs132981 TT genotype were significantly more frequent in UC patients than controls (P &lt; 0.05), whereas rs167281 showed no association with UC. Both rs110298 and rs132981 were linked to UC disease location and severity (P &lt; 0.05). UC patients exhibited significantly reduced serum vitamin D and elevated IL-17 levels compared with controls (P &lt; 0.05). Importantly, rs110298 (AG/GG) and rs132981 (TT) genotypes were associated with lower vitamin D and higher IL-17 concentrations in UC patients (P &lt; 0.05), indicating a biochemical effect of MTHFR variants on inflammatory and immunomodulatory pathways.</p><p><strong>Conclusions: </strong>MTHFR polymorphisms rs110298 and rs132981 are significantly associated with UC susceptibility and clinical phenotype and exert measurable biochemical effects on serum vitamin D and IL-17 levels. These findings highlight the potential value of MTHFR-related pathways as biomarkers for disease characterization and as mechanistic contributors to UC-related immune dysregulation.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1131-1139"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of Serum NT-pro-BNP, IGFBP-7 and CTRP12 levels in chronic heart failure patients.","authors":"Zhen Fan, Heng Huang, Laoji Wang, Jierou Yan, Fei Zhao","doi":"10.5937/jomb0-61393","DOIUrl":"10.5937/jomb0-61393","url":null,"abstract":"<p><strong>Background: </strong>To investigate serum N-terminal pro-brain natriuretic peptide (NT-pro-BNP), insulin-like growth factor binding protein-7 (IGFBP-7), and C1q tumour necrosis factor-related protein 12 (CTRP12) levels and significance in patients with chronic heart failure (CHF).</p><p><strong>Methods: </strong>The CHF group consisted of 116 CHF patients who received care at this hospital between October 2023 and March 2025. The patients were classified into Grade II (47 patients), Grade III (41 patients), or Grade IV (28 patients) based on the heart function classification of the New York Heart Association (NYHA). The control group consisted of 64 healthy patients who were physically examined in the hospital during the same period. Using multivariate logistic regression, the factors impacting MACEs in patients with congestive heart failure were examined. Through the use of the receiver operating characteristic (ROC) curve, researchers were able to assess the predictive power of blood NT-pro-BNP IGFBP-7, and CTRP12 for MACEs in chronic heart failure patients.</p><p><strong>Results: </strong>Comparing the CHF group to the control group, the LVEDD increased, the LVEF and serum CTRP12 decreased, and the levels of NT-pro-BNP, IGFBP-7, Hcy, and hs-CRP climbed (P&lt; 0.05). Among patients with CHF of different grades, serum NT-pro-BNP IGFBP-7, Hcy, hs-CRP and LVEDD levels were all lower in Grade II patients than in Grade III patients. Furthermore, the differences between any two grades were statistically significant (P&lt; 0.05). The levels of LVEF and serum CTRP12 in the MACE group decreased, the LVEDD increased, and the levels of serum Hcy, hs-CRP NT-pro-BNP and IGFBP-7 increased (P&lt; 0.05). MACEs in CHF patients were influenced by serum NT-pro-BNP IGFBP-7, and CTRP12 (P&lt; 0.05). The areas under the curve (AUCs) of serum NT-pro-BNP IGFBP-7, CTRP12 alone and the combination of the three for predicting MACEs in patients with CHF were 0.862 (95% CI: 0.786-0.919), 0.805 (95% CI: 0.721-0.872), and 0.860 (95% CI: 0.784-0.918) and 0.961 (95% CI: 0.908-0.988), respectively. The AUCs of the three combined predictions were significantly greater than those of the individual predictions of NT-pro-BNP IGFBP-7, and CTRP12 (Z = 3.050 , 3.883, 3.218, all P&lt; 0.05).</p><p><strong>Conclusions: </strong>Serum levels of IGFBP-7 and NT-pro-BNP increase in CHF patients, whereas the level of CTRP12 decreases. Additionally, once functional classification was applied, NT-pro-BNP IGFBP-7, and CTRP12 levels changed. The combined detection of these three parameters has better efficacy in predicting MACEs in patients with CHF.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1014-1022"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating IncRNA DRAIC as a potential serum biomarker for predicting lymphatic and vascular spread in pancreatic cancer.","authors":"Ying Che, Ting Zhang, Jing Li, Xiao Jiang, Chao Li, Wei Li","doi":"10.5937/jomb0-63713","DOIUrl":"https://doi.org/10.5937/jomb0-63713","url":null,"abstract":"<p><strong>Background: </strong>Pancreatic cancer is notorious for its aggressive behavior and poor prognosis, largely due to delayed diagnosis and early dissemination. Identifying reliable serum biomarkers could enhance early detection and risk stratification. Long non-coding RNAs (lncRNAs) have been recognised as regulators of tumour biology; however, the clinical and biochemical relevance of lncRNA DRAIC in pancreatic cancer remains unclear.</p><p><strong>Methods: </strong>Serum samples were obtained from 100 patients diagnosed with pathologically confirmed pancreatic cancer and 100 ageand sex-matched controls. Quantitative real-time polymerase chain reaction (qPCR) was employed to quantify the circulating DRAIC expression levels. We analysed the associations between serum DRAIC levels and clinicopathological characteristics, including vascular involvement and lymph node metastasis. Receiver operating characteristic (ROC) curve analyses were performed to assess the diagnostic and predictive performance of DRAIC. Paired serum samples obtained before and after surgical resection were additionally analysed.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"1149-1155"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of cytokine levels and peripheral blood T lymphocyte subsets in systemic vascular inflammatory disease.","authors":"Zhonghan Lin, Xuanyang Dong, Junye Chen, Biao Li","doi":"10.5937/jomb0-61241","DOIUrl":"10.5937/jomb0-61241","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To measure the levels of T-cell subsets andcytokines in the peripheral blood of patients with systemicvascular inflammatory disease and to analyse the influenceof different clinical characteristics of systemic vascularinflammatory disease patients on the levels of T-cell subsetsand cytokines in the peripheral blood.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The case group included 80 patients with systemicvascular inflammatory disease who were diagnosed and trea -ted at our hospital between January 2021 and December2024. The control group consisted of 40 healthy volunteersrecruited from our hospital's health assessment centre. Theproportions of helper T-cell (Th)1, Th17, and regulatory Tcell (Treg) subsets in peripheral blood were analysed usingflow cytometry. Serum levels of cytokine, including interleukin-2 (IL-2), interferon-g (IFN-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;γ&lt;/span&gt;), tumour necrosis factor-a(TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;), IL-4, IL-10, IL-17, and IL-6, were measured usingenzyme-linked immunosorbent assay (ELISA).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the control group, the expressionlevels of total T lymphoid subsets and CD8+ T and Th1 cellsin patients with systemic vascular inflammatory disease weresignificantly higher, and there was no statistically significantdifference in Th, Th17, or Treg cell expression between thegroups (P&gt;0.05). However, there were statistically significantdifferences between the two groups [68.75 (54.23, 80.32)]and [72.10 (62.23, 86.45)], [23.44 (12.89, 33.76)] and[31.46 (20.13, 45.26)], [10.67 (8.23, 12.35)] and [10.26,17.96 [25.39)], Z=-3.13, -4.54, -3.97 (all P values&lt;0.05).Compared with those in the control group, patients with systemic vascular inflammatory disease had significantly higherserum levels of IL-17, IL-6, TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, IL-4, and IFN-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;γ&lt;/span&gt;, whereastheir IL-10 levels were significantly lower. The differenceswere statistically significant (Z=-4.32, -5.01, -8.18, -8.70,-3.48, and -8.30). The P values were all &lt;0.05. Comparedwith that in patients without related manifestations, IL-6expression was noticeably higher in systemic vascular inflammatory disease patients with arthritic symptoms and thosewith active systemic vascular inflammatory disease, and thedifference was statistically significant {pg/mL: [3.23 (2.98,5.35)] compared with [10.51 (6.72, 23.21)], [6.32 (4.79,8.93)] compared with [9.68 (6.97, 18.73)], Z=5.47, 8.76,P values &lt;0.05}. Compared with those in patients withoutrelevant clinical manifestations, TNF-&lt;span style=\"color: rgb(","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"988-997"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between elevated iron status and the risk of preeclampsia: A systematic review and meta-analysis.","authors":"Changhong Li, Xiaohui Xu, Min Qian","doi":"10.5937/jomb0-64058","DOIUrl":"10.5937/jomb0-64058","url":null,"abstract":"<p><strong>Background: </strong>This review aims to conduct a comprehensive and systematic report of the currently published information on the association between elevated hepcidin levels and the risk of preeclampsia by meta-analysis of published prospective case-control studies or cross-sectional studies combined with follow-up to provide a comprehensive and reliable basis for early intervention and clinical decision making in the disease.</p><p><strong>Methods: </strong>We searched five databases such as Pubmed, Web of Science, Cochrane, China National Knowledge Infrastructure, and WAN FANG DATA to retrieve articles related to the relationship between elevated levels of hepcidin and the risk of preeclampsia up to February 2025. We calculated the standardized mean deviation (SMD) and 95% confidence interval (95% CI) for comparison using a random-effects model.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 5","pages":"963-974"},"PeriodicalIF":1.9,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum human trophoblast cell surface antigen and T-box protein 5 levels play a role in predicting adverse pregnancy outcomes in eclampsia.","authors":"Yao Li, Sutong Huang, Yan Xuan, Hao Shen, Weiwei Zhang, Jia Li","doi":"10.5937/jomb0-60246","DOIUrl":"https://doi.org/10.5937/jomb0-60246","url":null,"abstract":"<p><strong>Background: </strong>The levels of human trophoblast surface antigen (Trop-2) and T-box protein 5 (TBX5) in the serum of patients with preeclampsia were detected, and their value in predicting adverse pregnancy outcomes was analysed.</p><p><strong>Methods: </strong>A total of 196 pregnant women with preeclampsia who underwent prenatal examinations and delivered in our hospital from October 2021 to September 2024, as well as 196 healthy pregnant women, were selected as the eclampsia group and the control group, respectively. The 196 pregnant women with preeclampsia were divided into two groups according to their pregnancy outcome: the adverse outcome group and the good outcome group. Using the enzyme-linked immunosorbent assay (ELISA), the levels of serum Trop-2 and TBX5 were measured. Using multivariate logistic regression analysis, the factors impacting the various pregnancy outcomes of preeclamptic pregnant women were examined.</p><p><strong>Results: </strong>Compared with the control group, the serum TBX5 levels in the eclampsia group (2 0.8 6± 6 .4 5 mg/L vs. 15.34± 5.07 mg/L), as well as diastolic blood pressure, FBG, 24-hour urine protein, TG, systolic blood pressure and TC, were significantly greater. The levels of Trop-2 ((5.46± 1 .7 2) mg/L vs. (7 .37 ± 2.08 ) mg/L). The total incidences of preterm infants, fetal distress, placental abruption and adverse pregnancy outcomes in the eclampsia group were significantly greater than those in the control group (P&lt; 0.05). The serum TBX5 level in the adverse outcome group ((23.01± 6.66) mg/L vs. (1 9.53± 6.32) mg/L), as well as diastolic blood pressure, FBG, 24-hour urine protein, TG, systolic blood pressure, and TC, were significantly greater than those in the good outcome group. The levels of Trop-2 ((4.90± 1.61) mg/L vs. (5.81± 1.79) mg/L) and HDL-C decreased significantly (P&lt; 0.05); Trop-2 (OR 95% CI: 0.38 (0.28, 0.53)), TBX5 (OR 95% CI: 3.89 (1.88, 8.04)) and 24-hour urine protein (OR: 3.14 (1.82, 5.42)) were influencing factors for the pregnancy outcome of pregnant women with preeclampsia (P&lt; 0.05); and the AUCs of serum Trop-2, TBX5 and their combination in predicting the pregnancy outcome of pregnant women with preeclampsia were 0.79, 0.76 and 0.90, respectively. The combined prediction of the two had a greater predictive value than did Trop-2 or TBX5 alone (all P&lt;0.05).</p><p><strong>Conclusions: </strong>The serum Trop-2 level of pregnant women with preeclampsia is abnormally low, and the TBX5 level is abnormally high. The combination of the two can be used as a biological indicator for evaluating adverse pregnancy outcomes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"825-832"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of serum cytokine levels and immune inflammatory factors in cirrhotic portal hypertension.","authors":"Yafeng Yuq, Zhiqiong Lan, Zhiren Huang, Lingqin Hu","doi":"10.5937/jomb0-60996","DOIUrl":"10.5937/jomb0-60996","url":null,"abstract":"<p><strong>Background: </strong>To investigate alterations in immune-inflammatory factors and variables, including procalcitonin(PCT), prostaglandin E (PGE), and serum C-reactive protein (CRP) in individuals with cirrhotic portal hypertension.</p><p><strong>Methods: </strong>From March 2020 to March 2025, 81 patientswith cirrhotic portal hypertension and 50 healthy volunteerswere hospitalised to our hospital. These people were divided into two groups: the portal hypertension group and thecontrol group. Aspartate aminotransferase (AST), albumin(ALB), total bilirubin (TBIL), alanine aminotransferase(ALT), and portal vein inner diameter (Pvd) were measured.Serum cytokines, including CRP PCT, and PGE, weremeasured, as well as the percentages of CD 4+/CD 8+ Tcells and CD 3+ , CD 4+ , and CD8+ T lymphocytes.</p><p><strong>Results: </strong>The group with portal hypertension had considerablylower ALB than the control group (P&lt; 0.05), whereas indicators including ALT, AST, TBIL, and Dpv were significantlyhigher in the portal hypertension group than in the controlgroup (P&lt; 0.05). While cytokine markers, including CRPPCT, and PGE, were higher in the portal hypertension groupthan in the control group (P&lt; 0.05), the numbers of CD3 +and CD4+ T lymphocyte subsets and CD4+/CD8+ T lymphocytes were considerably lower in the portal hypertensiongroup. With increasing liver function classification, the proportions of CD3+ and CD4+ T lymphocyte subsets, as wellas CD4+/CD 8+ T lymphocytes, decreased in patients. However, there was a statistically significant (P&lt;0.05) increase in the levels of serum PCT, PGE, and CRP Spearmancorrelation analysis revealed that liver function classificationwas negatively correlated with immune indicators. CRP PCTand PGE levels were favourably connected with CD4+ andCD4+/CD 8+ values (P &lt; 0.05). Dpv was shown to have anegative association with CD4+/CD8+ T cells and a positive correlation with PCT and PGE levels (P&lt;0.05) accordingto Pearson correlation analysis.</p><p><strong>Conclusions: </strong>Patients with cirrhotic portal hypertensionmay experience immune dysfunction and cytokine disorders. These changes are related to liver function and portalvein pressure and can be used as reference indicators forevaluating a patient's condition in clinical practice.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"882-888"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of serum inflammatory mediators and immunoglobulin levels in children with severe asthma infected by viruses versus bacteria.","authors":"Xiaoxia Zhang, Guojiang Jian, Xiaoqun Zhong, Mao Li, Shifang Wan, Huan Liu, Xiangrui Chen","doi":"10.5937/jomb0-60933","DOIUrl":"10.5937/jomb0-60933","url":null,"abstract":"<p><strong>Background: </strong>By tracking fluctuations in inflammatory mediators and immunoglobulins (Igs) in bacterialversus viral-induced severe asthma (SA) cases, this research seeks to identify microbial-specific immune characteristics to guide individualized treatment.</p><p><strong>Methods: </strong>This analysis included 100 children hospitalized between March 2023 and April 2025 for severe acute SA exacerbations with confirmed single-pathogen infections (50 viral vs. 50 bacterial). Serum samples collected preand post-treatment were analyzed for inflammatory mediators (Th1/Th2/Th17 cytokines and TNF-a) and Ig profiles (total IgE and IgG subclasses). Comparative analysis was performed to identify intergroup differences.</p><p><strong>Results: </strong>At baseline, the viral group showed higher Th1/Th17 co-activation responses, whereas the bacterial group had elevated Th2 cytokines/TNF-a (P &lt; 0.05). Therapeutic intervention reduced all cytokine subsets, though Th1/Th17 co-activation suppression was more marked in the viral group (P&lt; 0.05), and Th2 decay lagged in the bacterial group. Besides, viral cases had elevated IgG1/IgG3; bacterial cases showed higher IgG2 (P&lt; 0.05). After treatment, the levels of IgG1 and IgG3 in the virus group continued to increase, but IgG2 did not change.</p><p><strong>Conclusions: </strong>Viral SA infections predominantly trigger Th1/Th17 co-activation-mediated inflammation and higher IgG1/IgG3. In contrast, bacterial infections favor a Th2-dominant profile with IgG2 elevation, resulting in slower inflammation resolution.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"833-840"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}