Journal of Medical Biochemistry最新文献

{"title":"Evaluation of measurement uncertainty results calculated by two different methods and total analytical error for ethanol testing.","authors":"Mehmet Akif Bozdayi, İsmet Gamze Bozdayı, Gökhan Çakırca","doi":"10.5937/jomb0-55093","DOIUrl":"10.5937/jomb0-55093","url":null,"abstract":"<p><strong>Background: </strong>The analytical performance metrics of ethanol testing are critically important due to their legal implications when presenting and interpreting results. Measurement uncertainty (MU) and total analytical error (TAE) are essential approaches for evaluating and improving the quality of measurement procedures. This study aimed to calculate MU and TAE values, which assess the reliability of ethanol test results from different perspectives, and to evaluate the impact of MU values, calculated using two different methods, on the legal threshold.</p><p><strong>Methods: </strong>MU values were calculated following the guidelines of Nordtest and ISO/TS 20914. TAE was determined using the formula TAE%=1.65×CV%+Bias%. External and internal quality data from ethanol testing conducted between July 1, 2022, and June 30, 2024, were used for calculations.</p><p><strong>Results: </strong>The expanded MU values for ethanol testing were 13.95% according to the Nordtest Guide, 10.94% for low level and 9.59% for high level according to the ISO/TS 20914 Guide. The calculated TAE values were 12.59 for low levels, 11.47 for high levels, and 12.57 overall. MU and TAE values for ethanol testing in our laboratory remained within the allowable total error (±20%) according to CLIA 2024.</p><p><strong>Conclusions: </strong>We believe each laboratory should report ethanol test results and their respective MU values, particularly when evaluating results close to legal thresholds. Furthermore, we suggest that scientific committees standardise the method for calculating MU and define a target limit.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"808-813"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between NLRP6 inflammasome and the levels of inflammatory cytokines IL-1b and IL-18 in serum of patients with Meniere's disease.","authors":"Mengxiao Li, Ying Zhou, Zhibiao Liu, Xin Jin","doi":"10.5937/jomb0-55001","DOIUrl":"10.5937/jomb0-55001","url":null,"abstract":"<p><strong>Background: </strong>Meniere's disease (MD) is a prevalent condition in otolaryngology, with its annual incidence rate increasing. Consequently, understanding the underlying mechanisms of MD is of significant importance. The aim is to investigate the relationship between serum levels of interleukin-1b (IL-1b) and interleukin-18 (IL-18), as well as the activation status of NLRP6 inflammasomes, in patients with Meniere's disease and to evaluate their correlation with the severity of the disease, to improve the treatment strategy of Meniere's disease.</p><p><strong>Methods: </strong>From March 2021 to December 2023, 75 MD patients were selected from the Affiliated Huaian No.1 People's Hospital of Nanjing Medical University for research, and a control group consisting of 75 age-matched healthy individuals was established. Each participant contributed a 5 mL peripheral venous blood sample, which was archived at -80 °C for subsequent analyses. The expression levels of NLRP6 messenger RNA in the blood samples were quantified using real-time fluorescence quantitative PCR methodology. Concentrations of interleukin-1b (IL-1b) and interleukin-18 (IL-18) were measured via enzyme-linked immunosorbent assay (ELISA). Through a comparative examination of NLRP6, IL-1b, and IL-18 levels between MD patients and the healthy controls, the study delved into the potential association between NLRP6 expression and the circulating levels of these two cytokines. In addition, special attention is paid to the differences between unilateral and bilateral MD patients in the above three indexes to evaluate their effectiveness as potential biomarkers for predicting the severity of hearing loss in MD patients.</p><p><strong>Results: </strong>In individuals suffering from MD, a notable elevation was observed in the peripheral blood expression levels of NLRP6, IL-1b, and IL-18 (p<0.001). A correlation assessment disclosed a positive association between the blood NLRP6 content and both IL-1b and IL-18 concentrations among these patients. Notably, no substantial disparity emerged in the expression profiles of these three biomarkers when comparing unilateral versus bilateral MD cases (p>0.05). Furthermore, patients at advanced stages (III+IV) exhibited significantly heightened levels of NLRP6, IL-1b, and IL-18 compared to their counterparts in earlier stages (I+II) (p<0.001). Receiver operating characteristic (ROC) curve analyses demonstrated that the area under the curve (AUC) for NLRP6, IL-1b, and IL-18 stood at 0.8731, 0.8089, and 0.7838, respectively, suggesting their potential as proficient diagnostic markers capable of differentiating MD patients from healthy controls.</p><p><strong>Conclusions: </strong>NLRP6, IL-1b, and IL-18 are highly expressed in the peripheral blood of MD patients. NLRP6, IL-1b, and IL-18 can serve as early diagnostic indicators for MD.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"740-747"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factorial analysis of zinc serum levels, fatty acids, oxidative stress parameters and supplementation on assisted reproductive technology outcome.","authors":"Aleksandra Veselinović, Jelena Kotur-Stevuljevć, Aleksandar Stojsavljević, Dragana Bojović-Jović, Aleksandra Arsić, Marija Paunović, Vesna Vučić","doi":"10.5937/jomb0-53112","DOIUrl":"10.5937/jomb0-53112","url":null,"abstract":"<p><strong>Background: </strong>Infertility remains a prevalent global reproductive challenge, significantly affecting the lives of couples worldwide. The aetiology of infertility can be affected by various factors that exhibit possible relationships with one another. The study aimed to investigate factors that can interact with and influence the pregnancy outcome in couples undergoing assisted reproductive technology procedures.</p><p><strong>Methods: </strong>This retrospective study included 64 couples (64 men and their female partners) undergoing assisted reproductive technology procedures, having different pregnancy outcomes and lifestyle habits. Biomarkers of antioxidative and fatty acid status in the serum of both male and female partners, as well as the concentration of zinc in serum and seminal plasma of men, and their impact on assisted reproductive technology outcome were examined.</p><p><strong>Results: </strong>We grouped the parameters using principal component analysis and identified the three most contributing factors to the pregnancy achievement - seminogram parameters in males and redox status scores in female participants; supplementation with vitamin D, magnesium, and zinc; and serum levels of omega-3 and omega-6 fatty acids.</p><p><strong>Conclusions: </strong>The study concluded that seminogram parameters, intake of micronutrients, and levels of zinc, omega-3, and omega-6 fatty acids are contributing factors to the success of assisted reproductive technology. Further studies on a larger cohort are needed to confirm the predictive role of these factors on the success of assisted reproductive technology.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"840-853"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complement pathway: Risk molecular pattern of cardiovascular diseases in patients with diabetic nephropathy.","authors":"JunFei Liu, XiaoPing Qian, TaoXia Wang, XiaoLi Liu, WeiGang Liu, LiJie Wang, HuiFang Zhang, Li Han, GuiYing Li, XiaoJuan Yu","doi":"10.5937/jomb0-56221","DOIUrl":"10.5937/jomb0-56221","url":null,"abstract":"<p><strong>Background: </strong>Among individuals with diabetes mellitus, diabetic nephropathy (DN) is a common microvascular complication. As renal dysfunction progresses in DN patients, the risk of cardiovascular disease (CVD) significantly increases. The current effective treatment of DN and CVD demands identifying and managing their risk factors.</p><p><strong>Methods: </strong>Patients diagnosed with DN from October 2022 to October 2023 were selected for cross-sectional study and divided into DN group (n=58) and DN/CVD group (n=40) based on the presence of CVD. Univariate analysis was conducted using clinical data, and statistically significant independent variables were analysed through multivariate Logistic regression to identify independent factors influencing CVD in DN patients. A regression model was developed to examine the non-linear relationship between C1q, UA, CRP, and the risk of CVD. The receiver operating characteristic curve was used to analyse the predictive efficacy of the indicators.</p><p><strong>Results: </strong>When elevated, UA and C1q were independent factors for CVD. A linear relationship existed between UA and C1q and the risk of CVD in DN patients. C1q showed better predictive performance.</p><p><strong>Conclusions: </strong>As UA and C1q levels rise, the risk of CVD in DN patients significantly increases. In DN patients, UA and C1q are associated with CVD development and progression, offering some supportive evaluation value for the patient's condition.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"801-807"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive levels of vascular endothelial growth factor (VEGF), thymidine kinase 1 (TK1) with interleukin-6 (IL-6), plasma T cells, NK cells as well as B cells in treating diffuse large B-cell lymphoma receiving rituximab.","authors":"Lihua Tian, Baoan Luo, Jun Tang, Jiagui Ye","doi":"10.5937/jomb0-54911","DOIUrl":"10.5937/jomb0-54911","url":null,"abstract":"<p><strong>Background: </strong>The aim was to explore the effect of rituximab in combination with chemotherapy in treating diffuse large B-cell lymphoma and levels of vascular endothelial growth factor (VEGF), thymidine kinase 1(TK1) with interleukin-6 (IL-6), plasma T cells, NK cells as well as B cells.</p><p><strong>Methods: </strong>Eighty patients admitted to Lujiang County People's Hospital from January 2022 to January 2024 were included. The control group accepted cyclophosphamide, doxorubicin, vincristine, and prednisone chemotherapy regimens. The research group was treated with rituximab based on the control group. The clinical effects, vascular endothelial growth factor (VEGF), thymidine kinase 1 (TK1) and interleukin-6 (IL-6) levels, lymphocyte subsets index, quality of life and occurrence of adverse reactions were compared in both groups.</p><p><strong>Results: </strong>The research group's total clinical effective rate was better than the control group's (P<0.05). After therapy, compared to the control group, vascular endothelial growth factor, thymidine kinase 1, and interleukin-6 levels in the research group presented lower (P<0.05), plasma T cells, natural killer cells along with B cells in the research group presented lower (P<0.05), and Quality of Life Core Questionnaire-Core 30 scores in the research group presented higher (P<0.05). There was no difference in adverse reactions between the two groups (P>0.05).</p><p><strong>Conclusions: </strong>Rituximab combined with chemotherapy is effective in treating DLBCL patients, which can reduce serum-related factors promoting immune function and quality of life. Our study may provide compelling evidence for supporting the therapeutic regimen of rituximab combined with chemotherapy in DLBCL patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"784-791"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of empagliflozin in patients over 65 with type 2 diabetes mellitus complicating cardiorenal syndromes type II and IV.","authors":"Mugen Cao, Qiuyan Lin, Liling Lin, Wenjie Zhang, Lifeng Zhang","doi":"10.5937/jomb0-54743","DOIUrl":"10.5937/jomb0-54743","url":null,"abstract":"<p><strong>Background: </strong>Cardiorenal syndrome (CRS) is a complex clinical condition that leads to deterioration in both cardiac and renal functions. Empagliflozin, a sodium-glucose cotransporter 2 (SGLT2) inhibitor, is a novel anti-diabetic drug that also improves cardiac and renal functions. However, little research exists on the efficacy and safety of empagliflozin in elderly type 2 diabetes mellitus (T2DM) patients with CRS. We aimed to evaluate the effectiveness and safety of empagliflozin in patients 65 and older with T2DM complicated by Type II and IV CRS.</p><p><strong>Methods: </strong>A randomised, prospective study was conducted involving 200 patients 65 and older diagnosed with T2DM and CRS who were admitted to the cardiovascular department of Fujian Provincial Governmental Hospital from January 2020 to January 2024. Patients were randomised into an experimental group (n=100) treated with empagliflozin 10mg/day and a control group (n=100) receiving standard care. Blood glucose, cardiac and renal function indicators, adverse reactions and major adverse cardiovascular events were compared between groups. T-tests, Mann-Whitney U tests, Wilcoxon signed-rank tests, and chi-square tests were performed appropriately.</p><p><strong>Results: </strong>After one-year follow-up, patients in the experimental group showed significant improvements in fasting blood glucose, glycated haemoglobin, serum creatinine, urinary microalbumin, NT-proBNP, left ventricular ejection fraction, and left ventricular end-diastolic diameter compared to the control group (P<0.05). Empagliflozin also reduced the incidence of major adverse cardiovascular events, with a non-significant increase in adverse reactions such as urinary tract infections and genital infections.</p><p><strong>Conclusions: </strong>Empagliflozin demonstrates efficacy in improving glycemic control and cardiorenal function in T2DM patients over 65 with CRS. However, the drug's effect on biomarkers of acute myocardial injury and thrombosis requires further investigation. This study contributes to the growing body of evidence supporting the use of SGLT2 inhibitors in the management of CRS and emphasises the need for larger-scale, long-term studies to confirm these findings.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"905-914"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of carbamazepine and levetiracetam on coagulation parameters: Prothrombin time, activated partial thromboplastin time, D-dimer, and fibrinogen levels.","authors":"Jiaohui Li, Chengyun Zhong, Guanji Bian","doi":"10.5937/jomb0-52052","DOIUrl":"10.5937/jomb0-52052","url":null,"abstract":"<p><strong>Background: </strong>Epilepsy is a prevalent neurological disorder, and evaluating its treatment strategies is highly significant. This study aimed to compare the effects of monotherapy with carbamazepine and levetiracetam on the results of coagulation tests, including prothrombin time, activated partial thromboplastin time, fibrinogen, and D-dimer levels, as well as on seizure control in patients with partial-onset epilepsy.</p><p><strong>Methods: </strong>A total of 89 patients diagnosed with POE and treated at our hospital between January 2023 and January 2024 were enrolled. The patients were divided into the carbamazepine group and the levetiracetam group. Blood coagulation parameters, including prothrombin time, activated partial thromboplastin time, fibrinogen, and D-dimer levels, were measured at baseline (before treatment) and at 1, 3, and 6 months after medication initiation. Additionally, the frequency and severity of epileptic seizures were recorded for each group.</p><p><strong>Results: </strong>In the carbamazepine group, prothrombin time, activated partial thromboplastin time, and D-dimer levels were significantly reduced at 1-, 3-, and 6-months post-treatment compared to pre-treatment levels. Conversely, these changes were less pronounced in the levetiracetam group. Fibrinogen levels decreased in both groups after treatment. The frequency of epileptic seizures was markedly reduced in all patients after treatment. There was no significant difference in seizure control rates between the carbamazepine and levetiracetam groups.</p><p><strong>Conclusions: </strong>Carbamazepine may pose a higher risk of coagulation abnormalities but demonstrated strong efficacy in controlling epileptic seizures. Levetiracetam had a milder impact on coagulation parameters while offering comparable effectiveness in seizure management.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"886-894"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of serum NLR, PLR, P53, K67 level in lymph node metastasis of early gastric cancer.","authors":"Xin Long, Yingxi Shi, Feifei Li, Zhaojun Wang, Ying Wang","doi":"10.5937/jomb0-55634","DOIUrl":"10.5937/jomb0-55634","url":null,"abstract":"<p><strong>Background: </strong>To explore the predictive value of relevant detection indexes and pathological serum NLR, PLR, P53, and K67 levels in lymph node metastasis (LNM) in patients with early gastric cancer (EGC) after radical surgery.</p><p><strong>Methods: </strong>Clinical data of EGC patients (297 cases, all of whom underwent radical gastrectomy for gastric cancer) admitted to Sichuan Integrative Medicine Hospital from March 2019 to March 2024 were retrospectively included. The clinical data and pathological results were recorded and compared, and the related predictive factors were analysed.</p><p><strong>Results: </strong>There were 43 cases (14.48%) of postoperative LNM among the 297 EGC patients. The average number of lymph nodes detected in the LNM (-) group was 28.35 ± 8.23, which was lower than in the LNM (+) group (33 ± 15), *P* < 0.01. Binary multivariate logistic regression analysis identified the following as significant predictors of postoperative LNM in EGC patients: tumour size (OR: 2.582, 95% CI: 1.205-5.534), depth of invasion (OR: 2.953, 95% CI: 1.327-6.573), vascular invasion (OR: 2.724, 95% CI: 1.241-5.976), neuroaggression (OR: 2.681, 95% CI: 1.139-6.311), differentiation type (OR: 2.426, 95% CI: 1.140-5.119), and P53 (OR: 3.133, 95% CI: 1.183-8.301), P<0.05. The area under the ROC curve (AUC) for the model based on these indexes was 0.801. Compared with the LNM (-) group, the LNM (+) group had a lower overall survival rate at 1 and 2 years (P<0.05).</p><p><strong>Conclusions: </strong>Clinically relevant detection indexes and pathological P53 levels in patients after EGC radical surgery have a good predictive effect on the occurrence of LNM, which can assist in formulating scientific and reasonable clinical treatment plans.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"792-800"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of improved ultrafiltration on serum level of IL-6 and TNF-a, HCT, and cardiopulmonary function in patients with extracorporeal circulation in valve replacement.","authors":"Geng Ning, Lili Fu, Guangwei Zhou","doi":"10.5937/jomb0-54272","DOIUrl":"10.5937/jomb0-54272","url":null,"abstract":"<p><strong>Background: </strong>To investigate the effects of modified ultrafiltration in extracorporeal circulation valve replacement surgery.</p><p><strong>Methods: </strong>A total of 62 patients with valvular disease who underwent valve replacement were included. They were randomly divided into the conventional ultrafiltration group (CUF group, n=31) and the modified ultrafiltration group (MUF group, n=31). The hematocrit (Hct) values, volume of pleural fluid drainage at 24 hours after operation, Intensive Care Unit (ICU) stay time, postoperative 24-hour blood loss, bank blood usage, postoperative 24-hour urine volume, ventilator support time, cardiac function indexes, postoperative changes of respiratory function, and levels of inflammatory factors in both groups were compared.</p><p><strong>Results: </strong>After ultrafiltration, the MUF group showed higher Hct value and reduced volume of pleural fluid drainage, blood loss, bank blood usage, urine volume and ventilator support time 24 hours after operation compared with the CUF group (P<0.05). After surgery, left ventricular ejection fraction (LVEF) levels were elevated, and those in the MUF group were higher than those in the CUF group. Left ventricular end-diastolic diameter (LVEDD) and heart rate (HR) were decreased in both groups after surgery. They were lower in the MUF group than in the CUF group (P<0.05). After ultrafiltration, the OI value in the MUF group was higher, and the alveolar-arterial oxygen partial pressure gradient (P (A-a)O2) value was lower than the CUF group (P<0.05). The plasma concentrations of interleukin 6 (IL-6) and tumour necrosis factor-alpha (TNF-a) were increased after cardiopulmonary bypass (CPB) in both groups and then decreased after ultrafiltration, and IL-6 and TNF-a levels in MUF group were lower than those in CUF group (P<0.05).</p><p><strong>Conclusions: </strong>MUF attenuates the postoperative systemic inflammatory response, reduces the lung injury caused by CPB, and improves the lung function of patients in the early postoperative period, which benefits patient recovery after surgery and is valuable in heart valve replacement.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"854-862"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroiditis and human blood metabolites: A mendelian randomization study.","authors":"Lijie Shao, Siqi Liu, Yongfu Song, Shaoyu Han, Yue Ma, Yang Kunpeng, Jingbin Zhang, Bingxue Qi, Yan Guo, Xiaodan Lu","doi":"10.5937/jomb0-56217","DOIUrl":"10.5937/jomb0-56217","url":null,"abstract":"<p><strong>Background: </strong>The risk factors for thyroiditis, an inflammatory disease with a complex etiology, remain poorly understood. Blood metabolites are known to change during thyroiditis development, suggesting a close relationship between blood metabolites and thyroiditis progression. However, evidence for a causal link is lacking. We employed Mendelian randomization (MR) methodology to systematically investigate the putative causal relationships between blood metabolite profiles and two clinically distinct thyroiditis phenotypes-subacute and autoimmune thyroiditis-providing insights into their metabolic underpinnings.</p><p><strong>Methods: </strong>We analyzed genomic and health data from 88 million Finnish Biobank participants in the Genome-Wide Association Study (GWAS). The primary analytical method was random-effects inverse variance weighting (IVW), supplemented by the weighted median method (WME) and Mr-Egger. We implemented comprehensive sensitivity analyses encompassing Cochran's Q test, Mr-Egger intercept, leave-one-out analysis (LOO), and Mr-PRESSO to assess heterogeneity, pleiotropy, and outliers. Extended genetic investigations incorporated the linkage disequilibrium score regression (LDSC) method, multivariable Mr (MVMR), and metabolic pathway analyses to provide deeper mechanistic insights.</p><p><strong>Results: </strong>Ten metabolites were significantly associated with autoimmune thyroiditis, and fifteen with subacute thyroiditis. Nonadecanoate (19:0) and 1-palmitoylglycerophosphoinositol* were found to directly affect subacute thyroiditis. MVMR analyses identified pelargonate (9:0), carnitine, and ADpSGEGDFXAEGGGVR* as having an independent and direct effect on autoimmune thyroiditis. Additionally, metabolic pathways such as neomycin, kanamycin, and gentamicin biosynthesis, histidine metabolism, and starch and sucrose metabolism were linked to autoimmune thyroiditis, while phenylalanine, tyrosine, tryptophan biosynthesis, phenylalanine metabolism, and arginine biosynthesis were associated with subacute thyroiditis.</p><p><strong>Conclusions: </strong>Our findings establish causal relationships between circulating metabolites and thyroiditis, revealing novel mechanistic insights through integrated genomic and metabolomic analyses. These results not only advance our understanding of thyroiditis pathogenesis but also suggest potential biomarkers for disease screening and therapeutic targets for intervention.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 4","pages":"872-885"},"PeriodicalIF":1.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12363355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}