Journal of Medical Biochemistry最新文献

{"title":"Phospholipid transfer protein: The link between lipid metabolism and inflammation in healthy pregnancy and pregnancy with cardiometabolic complications.","authors":"Marko Stanković, Jelena Munjaš, Minja Derikonjić, Aleksandra Željković, Jelena Vekić, Tamara Antonić, Daniela Ardalić, Milica Miljković-Trailović, Marija Šarić-Matutinović, Tamara Gojković, Snežana Jovičić, Željko Miković, Aleksandra Stefanović","doi":"10.5937/jomb0-64876","DOIUrl":"10.5937/jomb0-64876","url":null,"abstract":"<p><strong>Background: </strong>Phospholipid transfer protein (PLTP) regulates high-density lipoprotein (HDL) remodelling by transferring cholesterol and phospholipids and affecting particle size and function. Pregnancy alters lipid metabolism, but the adaptation of PLTP to pregnancy remains poorly understood. This study investigated longitudinal changes in PLTP gene expression and concentration during healthy pregnancy and pregnancy with cardiometabolic complications, and their associations with maternal lipid metabolism and inflammatory status.</p><p><strong>Methods: </strong>We followed 84 healthy and 20 pregnant women who developed gestational diabetes mellitus and hypertensive disorders during pregnancy. PLTP gene expression, PLTP concentration, advanced lipid profile parameters, and inflammatory status were analysed throughout the trimesters.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"841-855"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel cardiac biomarkers (serum S100A12 (Calgranulin C), FSTL1 (Follistatin-like 1), and osteocalcin) in patients with acute chest pain.","authors":"Wenjing Xie, Wei Zhao, Hongtao Wang, Yuanguang Xie, Luyan Gu","doi":"10.5937/jomb0-58940","DOIUrl":"10.5937/jomb0-58940","url":null,"abstract":"<p><strong>Background: </strong>To investigate the role of novel cardiac biomarkers S100A12 (Calgranulin C), FSTL1 (Follistatin-like 1), and osteocalcin in patients with acute chest pain (ACP) and evaluate their modulation by optimised emergency care protocols.</p><p><strong>Methods: </strong>A cohort of 116 ACP patients was divided into a research group (RG, n = 58) receiving optimised emergency care and a control group (CG, n = 58) receiving standard care. Serum levels of traditional biomarkers (troponin I, creatine kinase-MB [CK-MB], C-reactive protein [CRP], interleukin-6 [IL-6]) and novel biomarkers (S100A12, FSTL1, osteocalcin) were measured at baseline and post-intervention. Clinical outcomes, including triage time, hospital stay, and adverse events, were assessed to correlate with biochemical changes.</p><p><strong>Results: </strong>The RG exhibited significantly lower serum levels of troponin I, CK-MB, CRP, IL-6, S100A12, and FSTL1 post-intervention compared to the CG (P&lt; 0.05), indicating reduced myocardial injury and inflammation. Osteocalcin levels were higher in the RG (P&lt; 0.05), suggesting improved vascular and metabolic function. Clinically, the RG showed shorter triage times, reduced hospital stays, and lower adverse event rates (P&lt; 0.05).</p><p><strong>Conclusions: </strong>Optimised emergency care modulates novel biomarkers S100A12, FSTL1, and osteocalcin, alongside traditional markers, reflecting reduced cardiac stress and inflammation in ACP patients. These findings suggest a biochemical basis for improved clinical outcomes and highlight the potential of these biomarkers as diagnostic and prognostic tools in ACP management.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"731-739"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of serum intestinal fatty acid binding protein, D-lactic acid and intercellular adhesion factor-1 in sepsis patients with intestinal ischemia.","authors":"Yujie Shu, Bo Lao, Zhangzhang Song, Zhixing Zhuang, Hongzhang Li","doi":"10.5937/jomb0-61181","DOIUrl":"10.5937/jomb0-61181","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To analyse the correlations between the levels of serum intestinal fatty acid binding protein (I-FABP), D-lactic acid (D-Lac), and intercellular adhesion factor-1 (ICAM-1) in patients with sepsis complicated with intestinal ischemia-reperfusion injury and the degree of intestinal mucosal injury and patient prognosis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 236 patients with sepsis complicated with intestinal ischemia-reperfusion injury were included in the reperfusion injury group, and 176 patients with sepsis alone during the same period were included in the sepsis group. Based on the extent of intestinal mucosal damage, individuals in the reperfusion injury group were split into three groups: mild injury (52 patients), moderate injury (116 patients), and severe injury (68 patients). The patients were further divided into a good prognosis group (188 patients) and a poor prognosis group (48 patients). The general clinical data of all patients, including laboratory test indicators, were collected and grouped for comparison before and after treatment. Correlation analysis was conducted via Spearman correlation analysis. Predictive value assessment was conducted via receiver operating characteristic (ROC) curves.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The reperfusion damage group had higher levels of I-FABP D-Lac, ICAM-1, and IMA than the sepsis alone group, whereas the GLP-1 level was lower (P&lt; 0.05) than the sepsis alone group. Before treatment, the levels of I-FABP D-Lac, ICAM-1, and IMA in the mild, moderate, and severe injury groups increased successively, and the level of GLP-1 decreased successively (P&lt; 0.05). After treatment, the levels of I-FABP D-Lac, ICAM-1, and IMA in the three groups of patients were all lower than those before treatment in the same group, and the levels of the above indicators in the severe injury group were all greater than those in the mild and moderate injury groups during the same period. The GLP-1 levels of the three patient groups were all higher than those before treatment within the same group. In comparison to the mild and moderate damage groups, the severe injury group's GLP-1 levels were lower during the same time period (P&lt; 0.05). Spearman correlation analysis revealed that the levels of I-FABP D-Lac, ICAM-1, and IMA were positively correlated with the AGI grade. In contrast, the level of GLP-1 was negatively correlated with the AGI grade (P&lt; 0.05). While the level of GLP-1 was lower than that of the good prognosis group (P&lt; 0.05), the levels of I-FABP D-Lac, ICAM-1, and IMA were higher in the bad prognosis group. The ROC curve analysis revealed that the combined predictive value of I-FABP D-Lac, ICAM-1, IMA, and GLP-1 for poor prognosis in patients with sepsis complicated by intestinal ischemia-reperfusion injury was greater than that of these five indicators alone (P&lt; 0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The levels of serum I-FABP, D-Lac, ICAM-1, IMA, an","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"757-766"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of Th1/Th2 cytokines and liver fibrosis indicators in chronic hepatitis B patients.","authors":"Chun Yu, Meiling Chen, Xiaonan Han, Yuhang Feng, Hongwei Sun, Hong Yang","doi":"10.5937/jomb0-60938","DOIUrl":"10.5937/jomb0-60938","url":null,"abstract":"<p><strong>Background: </strong>To investigate the potential therapeutic benefits of autoimmune antibody detection in individuals suffering from chronic hepatitis B.</p><p><strong>Methods: </strong>In the observation group, 102 patients with chronic hepatitis B who were admitted between March 2022 and March 2025 were included. Additionally, the control group consisted of 102 healthy people who were examined throughout the same time period. The two groups' autoimmune antibodies were identified, and patients in the observation group with either positive or negative autoimmune antibodies were compared in terms of liver function, liver fibrosis markers, and cytokine levels.</p><p><strong>Results: </strong>The total positive rate of autoimmune antibodies in the observation group was 26.47%, whereas it was 3.92% in the control group (P&lt;0.05). Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were noticeably greater in patients in the observation group with positive autoimmune antibodies than in those with negative antibodies. However, the albumin (ALB) and total protein (TP) levels were much lower in these patients than in patients with negative antibodies. A statistically significant difference was observed (P&lt; 0.05). The levels of type III procollagen (PCIII), hyaluronidase (HA), and laminin (LN) were significantly (P&lt;0.05) greater in individuals with positive autoimmune antibodies than in those with negative antibodies. Patients with positive autoimmune antibodies had considerably higher levels of interleukin-4 (IL-4), interleukin-10 (IL-10), and interleukin-6 (IL-6) than patients with negative antibodies. Patients who were negative for antibodies had significantly higher levels of interferon-y (IFN-y) (P&lt;0.05).</p><p><strong>Conclusions: </strong>Autoimmune antibodies, which influence liver fibrosis indicators and related cytokines, are present in patients with chronic hepatitis B. The identification of autoimmune antibodies in chronic hepatitis B patients can serve as a guide for evaluating illness and determining patient prognosis.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"749-756"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum cystatin C as a biomarker in non-Hodgkin lymphoma and its relationship with paraoxonase 1 activity.","authors":"Bosa Mirjanić-Azarić, Smiljana Mijić, Zana Radić-Savić, Siniša Stanković, Dragana Malčić-Zanić, Egeljić Nataša Mihailović, Đorđe Stojisavljević, Bojana Ivetić, Nataša Bogavac-Stanojević","doi":"10.5937/jomb0-61502","DOIUrl":"10.5937/jomb0-61502","url":null,"abstract":"<p><strong>Background: </strong>This pilot study aimed to assess the potential of cystatin C (Cys C) as a biomarker in non-Hodgkin lymphoma (NHL) and to explore its association with paraoxonase 1 (PON1) activity.</p><p><strong>Methods: </strong>The study included 44 patients with B-cell NHL and 44 healthy subjects. Cys C was measured using the Cobas e 801 analyser (Roche Diagnostics GmbH, Mannheim, Germany), while PON1 and TAS were measured on the ILab 300+ system (Instrumentation Laboratory, Milan, Italy).</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"889-896"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined detection of CXCR7 and CXCL12 for diagnosing acute myocardial infarction and predicting post-percutaneous coronary intervention major adverse cardiovascular events.","authors":"Hong Pan, Xiaoli Fan, Yu Liu, Gulijiakela Aishan, Jingwen Shao","doi":"10.5937/jomb0-61266","DOIUrl":"10.5937/jomb0-61266","url":null,"abstract":"<p><strong>Background: </strong>To explore the diagnostic utility of combined CXCR7 and CXCL12 testing in acute myocardial infarction (AMI) and its predictive value for post-percutaneous coronary intervention (PCI) major adverse cardiovascular events (MACE).</p><p><strong>Methods: </strong>This study enrolled 164 AMI patients and 54 healthy controls from January to June 2024. All patients received standardized PCI and were followed for 12 months. Serum CXCR7 and CXCL12 concentrations were determined via enzyme-linked immunosorbent assay (ELISA), while NT-proBNP was quantified with an automated biochemical analyzer. Comparative analyses of biomarker levels between groups were conducted, along with receiver operating characteristic (ROC) curve analysis for combined diagnostic performance. Dynamic changes in CXCR7/CXCL12 were also assessed in relation to postoperative MACE.</p><p><strong>Results: </strong>Pre-treatment concentrations of CXCR7 and CXCL12 were markedly higher in the AMI group relative to controls. The combined detection achieved an AUC value of 0.812 for diagnosing AMI, superior to individual indicators (P&lt; 0.05). Both markers decreased significantly following PCI (P&lt; 0.05). Those who developed MACE exhibited higher post-treatment CXCR7 and CXCL12 levels compared to non-MACE cases; their combined AUC reached 0.806 for predicting post-PCI MACE (P&lt; 0.05). While NT-proBNP demonstrated strong diagnostic accuracy for AMI (AUC = 0.893), it failed to predict post-PCI MACE (P&gt; 0.05). This discrepancy may reflect NT-proBNP's primary association with acute myocardial injury rather than chronic microvascular dysfunction or endothelial repair processes implicated in MACE.</p><p><strong>Conclusions: </strong>The favorable diagnostic performance of CXCR7 and CXCL12 for AMI and post-PCI MACE suggests their potential as a novel protocol for the clinical assessment of AMI.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"785-792"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical alterations in endocrine and inflammatory pathways following breast cancer surgery: Laboratory evidence and clinical implications.","authors":"Yafang Luo, Jinglan Xu, Hui Chen","doi":"10.5937/jomb0-63067","DOIUrl":"10.5937/jomb0-63067","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer surgery induces substantial physiological stress that disrupts endocrine regulation and triggers systemic inflammation. However, the biochemical dynamics underlying these perioperative alterations remain insufficiently characterized. This study investigated laboratory-based endocrine and inflammatory biomarker changes following breast cancer surgery and explored their clinical significance.</p><p><strong>Methods: </strong>A total of 120 breast cancer patients were evaluated. Serum estradiol (E2), progesterone (P), interleukin-6 (IL-6), C-reactive protein (CRP), and tumor necrosis factor-a (TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span>) were measured preoperatively and on postoperative day 7 using validated ELISA assays with strict analytical quality control. Clinical outcomes - including psychological status, cognitive recovery, quality of life, and postoperative complications - were assessed as secondary indicators to explore potential biochemical-clinical associations.</p><p><strong>Results: </strong>Postoperative endocrine suppression was evident, with significant reductions in E2 and P levels (P &lt; 0.05), reflecting acute stress-related modulation of hypothalamic-pituitary-gonadal (HPG) axis activity. IL-6, CRP and TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> declined significantly postoperatively (all P &lt; 0.001), demonstrating resolution of acute-phase inflammatory activation. Greater biochemical stabilization was associated with improved emotional scores, cognitive function, and quality-of-life indices, as well as a reduced incidence of postoperative complications.</p><p><strong>Conclusions: </strong>Breast cancer surgery induces characteristic biochemical changes involving endocrine suppression and systemic inflammatory activation. Monitoring hormonal and inflammatory biomarkers provides valuable insight into postoperative physiological stress, recovery mechanisms, and potential risk stratification. These findings support integrating laboratory-based biochemical assessments into postoperative management frameworks for breast cancer patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"874-881"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of sampling time on thyroid-stimulating hormone, free thyroxine, and free triiodothyronine levels.","authors":"Mahmut Mert Daglar, Asuman Orçun","doi":"10.5937/jomb0-61454","DOIUrl":"https://doi.org/10.5937/jomb0-61454","url":null,"abstract":"<p><strong>Background: </strong>The timing of sample collection is crucial for analytes with a circadian rhythm. Although blood samples are usually collected in the morning, they can also be collected in the afternoon. Our study aims to evaluate the impact of sampling time on Thyroid-Stimulating Hormone (TSH), free thyroxine (fT4), and free triiodothyronine (fT3).</p><p><strong>Methods: </strong>Serum TSH, fT4, and fT3 measurements from 216,278 patients who visited our institute's outpatient clinics over two years (2021-2023) were obtained from laboratory data. After exclusions, 5,953 patient results collected from 8 am to 5 pm were grouped into 1-hour intervals. All measurements were performed using the Cobas e801 (Roche Diagnostics, Penzberg, Germany) with the electrochemiluminescence immunoassay method. Statistical significance was tested with the standard normal deviation test (Z-test), and clinical significance was assessed using bias % values obtained from biologic variation estimates on the website of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM).</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"897-906"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of serum PACAP-38, fibulin-3 and TEM1 levels with cardiac function and prognosis in chronic heart failure patients.","authors":"Yan Chen, Fanlong Zeng, Tianyun Gao, Bayi Hu, Mo Zhang, Zongliang Yu","doi":"10.5937/jomb0-61365","DOIUrl":"https://doi.org/10.5937/jomb0-61365","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To analyse the relationships between the levels of serum fibrinum-3 (Fibulin-3), anti-human tumour endothelial marker 1 (TEM 1), and pituitary adenylate cyclase-activating polypeptide-38 (PACAP-38) in elderly chronic heart failure (CHF) patients and the classification of cardiac function and an unfavourable prognosis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 132 elderly CHF patients who were admitted to the hospital between July 2022 and July 2024 were chosen as the elderly CHF group, and 132 healthy people who were examined in the hospital during that time were selected as the control group. According to the cardiac function classification criteria of the New York Heart Association (NYHA), Grade II (42 patients), Grade III (51 patients), and Grade IV (39 patients) groups were created from the elderly CHF patients. Based on whether MACE happened after discharge, elderly CHF patients were split into two groups: those who experienced MACE and those who did not. The levels of serum Fibulin-3, TEM1 and PACAP-38 in all the subjects were detected via ELISA. Serum Fibulin-3, TEM 1, and PACAP-38 levels were correlated with cardiac function categorisation in older CHF patients using Spearman correlation analysis. Older patients with CHF had their MACEs examined using multivariate logistic regression analysis. Serum Fibulin-3, TEM 1, and PACAP-38 were evaluated for their predictive value of MACEs in older individuals with CHF.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;While the elderly CHF group's serum levels of Fibulin-3 and PACAP-38 were lower than those of the control group, their serum levels of TEM1 were higher. P&lt;0.05 indicated that the differences were statistically significant. Serum TEM1 levels are compared across various cardiac function categories in elderly CHF patients. Serum Fibulin-3 and PACAP-38 levels in older CHF patients with varying cardiac function grades were compared, revealing the following pattern: Grade II &gt; Grade III &gt; Grade IV ! Spearman correlation analysis revealed a favourable correlation between the serum TEM1 level and the heart function classification of elderly CHF patients (rs=0.488, P&lt;0.05), as well as between Fibulin-3 and PACAP-38 (rs=-0.463, -0.432, P&lt;0.05). The MACE group had lower levels of serum Fibulin-3 and PACAP-38 compared to the non-MACE group, while the levels of serum TEM1 and the proportion of patients with cardiac function grade IV were higher than in the non-MACE group. Multivariate logistic regression analysis revealed that elevated levels of serum Fibulin-3 and PACAP-38 were protective factors against MACEs. In contrast, elevated levels of serum TEM1 and cardiac function grade IV were risk factors for MACEs. According to the ROC curve study, the areas under the curve (AUCs) of serum Fibulin-3, TEM1, and PACAP-38 alone and the combination of the three indicators for predicting MACEs in patients with CHF were 0.666, 0.636, 0.641, and 0.798, respectively. The AUC pr","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"917-926"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149038895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of PGC-1β, HIF-1α and RETN with the degree of joint destruction in gouty arthritis.","authors":"Jian Bian, Jianguo Zhang, Bo Zhang, Hongfei Shi","doi":"10.5937/jomb0-60976","DOIUrl":"https://doi.org/10.5937/jomb0-60976","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To explore the expression levels of resistin (RETN), hypoxia-inducible factor-la (HIF -l&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt; ), and peroxi-some proliferator-activated receptor g coactivator-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt; (PGCip ) in gouty arthritis (GA) patients and to analyse their cor-relations with the degree of joint destruction.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The GA group consisted of 134 patients with GA who were admitted to the hospital between January 2023 and October 2024, while the control group consisted of 134 healthy patients who underwent physical examination in the hospital over the same time period. Serum PGC-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, HIF-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, and RETN expression levels were compared. The expression levels of PGC-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, HIF-1a and RETN in the serum and synovial fluid of patients with different clinical characteristics in the GA group were compared. The degree of joint destruction was categorised into 78 cases in the severe GA subgroup and 56 cases in the mild GA subgroup based on the VAS score of the chief complaint pain scale. Compared with PGC-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, HIF-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, RETN, and bone destruction factors [&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;-crosslinking degradation products (P-CTX), tartrate-resistant acid phosphatase-5b (TRACP5b), and nuclear factor p receptor activator ligand (RANKL)], and inflammatory factors with different degrees of joint destruction, the expression levels of 1p (IL-1 &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;) were analysed. The correlations between PGC-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, HIF-1&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, and RETN in serum and synovial fluid, as well as the degree of joint destruction, bone destruction factors, and inflamma-tory factors, were analysed.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The expression level of serum PGC-1&lt;span style=\"color: rgb","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 4","pages":"907-916"},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}