Journal of Medical Biochemistry最新文献

{"title":"Effect of progesterone sequential therapy on follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) in perimenopausal dysfunctional uterine bleeding.","authors":"Song Wu, Huiru Wang, Yuwei Zhao, Bo Tang, Qi Zhang, Dapeng Li","doi":"10.5937/jomb0-53229","DOIUrl":"10.5937/jomb0-53229","url":null,"abstract":"<p><strong>Background: </strong>Perimenopausal dysfunctional uterine bleeding (PDUB) is a common gynaecological disease with various clinical treatment options. The objective of this work was to investigate the clinical effect of female progesterone sequential therapy (FPST) on PDUB and follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2).</p><p><strong>Methods: </strong>140 cases of PDUB patients were enrolled and randomly rolled into an observation (Obs) group and a control (Ctrl) group, with 70 cases in each. The patients in the Ctrl group were given pure curettage treatment, and those in the Obs group were supplemented with FPST based on the intervention in the Ctrl group.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"653-659"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum brain-derived neurotrophic factor, IL-6, IL-10, and TNF-a cognitive function, and sleep quality in elderly patients with major depressive disorder and somatic symptoms.","authors":"Ya Gao, Xu Liu, Yan Gu","doi":"10.5937/jomb0-53387","DOIUrl":"10.5937/jomb0-53387","url":null,"abstract":"<p><strong>Background: </strong>The aim of the paper was to analyze the relationship between somatization in elderly patients with major depressive disorder (MDD) and brain-derived neurotrophic factor (BDNF), IL-6, IL-10, and TNF-a cognitive function, and sleep quality (SQ).</p><p><strong>Methods: </strong>This was a case-control study on 80 elderly patients with MDD who were grouped based on the somatic self-rating scale (SSS) - the subjects with somatic symptoms (SS) as the AG and the subjects without SS as the BG. Additionally, 25 healthy volunteers from the same period were included as the control group (CG). The subjects' SQ scores, BDNF and its precursor (ProBDNF), and cognitive function scores were collected.</p><p><strong>Results: </strong>The SQ and SS scores of AG and BG were visibly higher than against CG, and those of AG were visibly higher in contrast to BG. The cognitive function scores of AG and BG were visibly lower than against CG, and the score of AG was lower than BG (P<0.05). There was a similarity in BDNF and ProBDNF levels between AG and BG (P>0.05). CRP, IL-6, IL-10, and TNF-a in both AG and BG were visibly higher as against CG; Those were visibly higher in AG as against BG (P<0.05). The SS scores of the patients suggested a visible negative correlation with cognitive function scores and an evident positive correlation with SQ scores.</p><p><strong>Conclusions: </strong>Somatization symptoms may affect the SQ and cognitive function of people with depression, leading to an exacerbation of inflammatory responses; BDNF and ProBDNF levels may be more influenced by the overall state of depression rather than being determined solely by SS.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"568-575"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring anthropometric, biochemistry and nutritional attributes in overweight and obese women: Insights from the Serbian cohort: A pilot study.","authors":"Anka Petrović, Nevena Ivanović, Neda Milinković, Danijela Milenković, Snežana Polovina, Milica Zeković, Brižita Đorđević","doi":"10.5937/jomb0-52980","DOIUrl":"10.5937/jomb0-52980","url":null,"abstract":"<p><strong>Background: </strong>Anthropometric measurements (AMs), such as waist circumference and body mass index (BMI), are dominant indicators of overweight and obesity. Nevertheless, other AMs, such as waist-hip ratio, waist circumference and indices of visceral adiposity index (VAI), body mass fat index (BMFI), cardiometabolic index (CMI) and waist-to-height ratio (WtHR) are more significant and discriminatory than BMI in predicting cardiometabolic risk.</p><p><strong>Methods: </strong>This pilot study aimed to comprehensively investigate the anthropometric, biochemical and nutritional characteristics of a sample of overweight and obese women in Serbia, focusing on potential differences related to AM and its association with specific biochemical markers.</p><p><strong>Results: </strong>The final analytical sample consisted of 57 females (average age 37.16±7.27 years, range: 21-55 years). A strong positive correlation was observed between triglycerides (TG) and both the VAI (r=0.896, p<0.001) and the CMI (r=0.896, p<0.001), both with high statistical significance. Conversely, a strong negative correlation was found between high-density lipoprotein cholesterol (HDLc) and both VAI (r=-0.601, p<0.001) and CMI (r=-0.566, p<0.001). Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were significantly positively correlated with both WtHR and BMFI: SBP and WtHR (r=0.343, p=0.009), SBP and BMFI (r=0.310, p=0.019), DBP and WtHR (r=0.368, p=0.005), and DBP and BMFI (r=0.377, p=0.004). The analysis of biochemical parameters indicated no significant differences between groups, except for TG levels, which were significantly higher in the obese group (p=0.045).</p><p><strong>Conclusions: </strong>Although the AMs of the subjects were significantly different, energy intake and macronutrient intake were not significantly different. We did not find significant differences in the intake of most vitamins or minerals between the groups. Additionally, there is inadequate intake of essential nutrients such as vitamin D, iron and selenium, which are critical for overall health. Both groups did not meet the recommended folate intake, which may increase the risk of fetal neural tube defects.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"637-652"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylprednisolone effects on serum biochemical factors (CRP, PCT, IL-6, TNF-a) in viral pneumonia.","authors":"Hongping Zeng, Qi Zhu, Shaoyu Bai, Jie Liu, Dengjie Ren, Xin Chen","doi":"10.5937/jomb0-53124","DOIUrl":"10.5937/jomb0-53124","url":null,"abstract":"<p><strong>Background: </strong>This study was performed to demonstrate the effects of methylprednisolone (MPS) and azithromycin treatment on serum biochemical factors and their impact on Serum Biochemical Factors (CRP, PCT, IL-6, TNF-a) prognosis in patients with viral pneumonia (VP).</p><p><strong>Methods: </strong>This was a non-randomised clinical trial study on 120 patients with VP admitted to our hospital who had received different doses of methylprednisolone for viral pneumonia. Subjects were collected in four groups of Controls (Ctrl), low-dose (40 mg MPS, L-MPS), medium-dose (80 mg MPS, M-MPS), and high-dose (120 mg MPS, H-MPS) groups. The therapeutic efficacy of each group was evaluated. C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) were detected. Pulmonary function parameters were assessed using a pulmonary function testing device. Adverse reactions (ARs) such as fever, nausea, vomiting, and gastrointestinal bleeding post-treatment were recorded.</p><p><strong>Results: </strong>The total effective rate (TER) post-treatment in the Ctrl group was 60.00%, which was inferior to that in the L-MPS group (76.67%), M-MPS group (90.00%), and H-MPS group (93.33%) (P<0.05). Disappearance time of CRP, PCT, IL-6, TNF-a, fever, cough, and X-ray infiltrates was reduced in L-MPS, M-MPS, and H-MPS groups relative to the Ctrl group (P<0.05), while FVC, MMEF, and PEF were increased (P<0.05). Disappearance time of CRP, PCT, IL-6, TNF-a, fever, cough, and X-ray infiltrates in M-MPS and H-MPS groups was inferior to that in the L-MPS group (P<0.05), while FVC, MMEF, and PEF were higher (P<0.05).</p><p><strong>Conclusions: </strong>Medium-dose (80 mg) MPS combined with azithromycin greatly reduces inflammatory cytokine levels, shortens the time to clinical symptom relief, and improves lung function and respiratory capacity, demonstrating significant efficacy in treating VP.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"622-630"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mild hypothermia effects on serum neuroprotection, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and superoxide dismutase (SOD) levels in neonates with hypoxic-ischemic encephalopathy.","authors":"Wenfeng Duan, Xuan Wang","doi":"10.5937/jomb0-50866","DOIUrl":"10.5937/jomb0-50866","url":null,"abstract":"<p><strong>Background: </strong>Neonatal hypoxic-ischemic encephalopathy (HIE) is a serious condition that can lead to long-term neurological damage. Mild hypothermia is a promising treatment for HIE, but its efficacy and safety in newborns are not well established. To evaluate the therapeutic effects of mild hypothermia on neonatal HIE in a randomised controlled trial.</p><p><strong>Methods: </strong>This was a prospective study of 132 newborns with HIE treated with either mild hypothermia or routine conventional treatment. The primary outcome measures were changes in neural cytokines, brain injury markers, oxidative stress factors, neurological function recovery time, and therapeutic outcomes.</p><p><strong>Results: </strong>The mild hypothermia group showed significant improvements in neural cytokines (NGF and BDNF), brain injury markers (S100B, NSE, and MBP), and oxidative stress factors (SOD, MDA, IL-18, and caspase-3) compared to the control group. The mild hypothermia group also had a faster neurological function recovery time and a higher total response rate (95.45% vs. 80.30%, P<0.05) compared to the control group.</p><p><strong>Conclusions: </strong>Mild hypothermia therapy is a safe and effective treatment for neonatal HIE, with significant improvements in neural cytokines, brain injury markers, and oxidative stress factors, as well as faster neurological function recovery time and higher therapeutic outcomes. Results: The mild hypothermia group showed significant improvements in neural cytokines (NGF and BDNF), brain injury markers (S100B, NSE, and MBP), and oxidative stress factors (SOD, MDA, IL-18, and caspase-3) compared to the control group. The mild hypothermia group also had a faster neurological function recovery time and a higher total response rate (95.45% vs. 80.30%, P<0.05) compared to the control group. Conclusions: Mild hypothermia therapy is a safe and effective treatment for neonatal HIE, with significant improvements in neural cytokines, brain injury markers, and oxidative stress factors, as well as faster neurological function recovery time and higher therapeutic outcomes. The novelty of this work was that it showed potential biomarkers for evaluating response to treatment and the pathophysiological effect of treatment by assessing these biomarkers.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"515-523"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary analysis of the diagnostic value and mechanism of action of TGFbI and S100A4 in hepatocellular carcinoma.","authors":"Liang Zhao, Qinghua Shu, Bowen Sha, Miao Wu, Yufeng Zhang","doi":"10.5937/jomb0-54550","DOIUrl":"10.5937/jomb0-54550","url":null,"abstract":"<p><strong>Background: </strong>The aim was to analyse the diagnostic value of transforming growth factor-beta-induced protein (TGF I) and S100 calcium-binding protein A4 (S100A4) on hepatocellular carcinoma (HCC) and to explore further the effects of TGF I and S100A4 on ferroptosis in HCC cells.</p><p><strong>Methods: </strong>We retrospectively analysed 76 patients with HCC admitted to our hospital from October 2022 to June 2023 and detected the differences in the expression of TGF I and S100A4 in cancerous tissues and paracancerous tissues to analyse their diagnostic and prognostic assessment value for HCC. Additionally, the HCC cell line HepG2 was purchased and transfected with TGF I and S100A4 abnormal expression plasmids to check changes in cell viability, oxidative stress damage, mitochondrial damage, and ferroptosis.</p><p><strong>Results: </strong>TGF I and S100A4 were upregulated in HCC tissues (P<0.05), and their combined detection exhibited excellent diagnostic effects for HCC. The levels of TGF I and S100A4 in patients who died prognostically were higher than those in surviving patients (P<0.05). An increase in the levels of TGF I and S100A4 indicates an elevated risk of prognostic death in patients. Upregulating TGF I and S100A4 expression in cell experiments activated HepG2 activity, inhibited apoptosis, mitochondrial and oxidative stress damage, and improved cell ferroptosis.</p><p><strong>Conclusions: </strong>TGF I and S100A4 are elevated in HCC and can potentially be clinical diagnostic indicators of HCC.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"400-411"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the effects of menopause and obesity on matriptase-2 and neurokinin B concentrations: A cross-sectional study.","authors":"Arikan Funda Bulut, Nevin Sagsoz","doi":"10.5937/jomb0-52348","DOIUrl":"10.5937/jomb0-52348","url":null,"abstract":"<p><strong>Background: </strong>Matriptase-2 (MT2) is implicated in iron metabolism, obesity, insulin resistance, and glucose homeostasis. Neurokinin B (NKB) plays a crucial role in gonadotropin secretion, which is essential for reproduction. The impact of menopause and obesity on MT2 and NKB concentrations remains an enigma. Therefore, the present study sought to investigate how obesity and menopause affect serum concentrations of MT2 and NKB.</p><p><strong>Methods: </strong>In this cross-sectional study, 120 female volunteers were divided into four groups: menopausal overweight, menopausal obese, menopausal normal weight (control), and reproductive normal weight (control). The participants' serum levels of MT2, NKB, iron, ferritin, and relevant biochemical parameters were analysed using the ELISA method.</p><p><strong>Results: </strong>The mean serum concentrations of NKB, MT2, and ferritin were higher in the menopausal overweight and obese groups (p<0.05). The mean concentrations of NKB, MT2, and estradiol were determined to be higher, and the levels of iron, hemoglobin, glucose, FSH, LH, LDL, and total cholesterol were found to be lower in the reproductive control group when compared with the menopausal control group (p<0.05). A positive correlation was identified between BMI and NKB, MT2, and ferritin concentrations in menopausal overweight and obese groups (p<0.05).</p><p><strong>Conclusions: </strong>Obesity elevated NKB and MT2 concentrations in menopausal women. The increase in MT2 levels in menopausal overweight or obese women may be one of the factors responsible for the increase in ferritin and body fat ratio. As BMI raised, NKB, MT2, and ferritin concentrations raised in the menopausal overweight or obese groups; therefore, depression of MT2 in obese patients could be beneficial for therapeutic purposes. Additionally, gonadal hormonal changes altered serum concentrations of NKB, and the increase in NKB and gonadotropin levels in menopause may enhance vasomotor symptoms.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"470-478"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of FV G1691A, FV H1299R, and FII G20210A variations with thrombosis and coronary artery disease (CAD): A population-based study.","authors":"Özmen Sevda Ünallı, Yeşim Özarda, Aylin Köseler, Ramazan Sabırlı, Derya Sucu Kaynak, Ibrahim Koç","doi":"10.5937/jomb0-39668","DOIUrl":"10.5937/jomb0-39668","url":null,"abstract":"<p><strong>Background: </strong>Thrombosis and coronary artery disease (CAD) are complex disorders influenced by genetic factors. Specific gene variations, such as Factor V (FV) G1691A (Leiden), FV H1299R, and Prothrombin (FII) G20210A, have been implicated in thrombotic events and CAD. However, their precise role in CAD development remains controversial. This study investigated the prevalence and association of these gene variations with thrombosis and CAD in the Turkish population.</p><p><strong>Methods: </strong>A case-control study included 406 healthy individuals and 64 CAD patients. Genotyping for FV G1691A, FV H1299R, and FII G20210A was performed using a strip assay. Fisher's exact test compared allele and genotype frequencies between the CAD and control groups.</p><p><strong>Results: </strong>No significant differences were observed in genotype frequencies of FV G1691A, FV H1299R, and FII G20210A between the CAD and control groups (p>0.05). Similarly, allele frequencies did not differ significantly between the two groups (p>0.05).</p><p><strong>Conclusions: </strong>The findings suggest that FV G1691A, FV H1299R, and FII G20210A variations may not play a significant role in the development of CAD in the Turkish population studied. These results are consistent with the existing conflicting literature on the association between these gene variations and CAD. Further research with larger sample sizes and diverse populations is warranted to elucidate the role of these variations in CAD pathogenesis.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"447-452"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of HMGB-1 and acetylcholinesterase in assessing the prognosis of patients with acute pancreatitis.","authors":"Guofei Peng, Wanfang Chen, Yan Li, Danping Zhang","doi":"10.5937/jomb0-53786","DOIUrl":"10.5937/jomb0-53786","url":null,"abstract":"<p><strong>Background: </strong>Acute pancreatitis (AP) is a disorder of tissue digestion caused by abnormal activation of pancreatic enzymes, which may lead to multi-organ failure and, ultimately, death as the disease progresses. How to quickly and accurately evaluate the progression of AP has always been a hotspot and difficulty in clinical research.</p><p><strong>Methods: </strong>Sixty-four AP patients were admitted to our hospital between August 2022 and June 2023, and 60 healthy people during the same period were selected for retrospective analysis, with AP patients as the observation group and healthy people as the control group. HMGB-1 and AChE levels were detected in both groups, and the diagnostic value of HMGB-1 and AChE for AP was analysed using the receiver operating characteristic (ROC) curve. Subsequently, the differences in the expression of HMGB-1 and AChE in AP patients with different severities were further observed. In addition, we detected albumin (ALB), transferrin (TRF), and total protein (TP) in the observation group and analysed their correlation with HMGB-1 and AChE. Finally, a 6-month prognostic follow-up was performed to analyse the predictive value of HMGB-1 and AChE for poor prognosis in AP using ROC curves.</p><p><strong>Results: </strong>Compared with the control group, HMGB-1 was higher in the observation group, which was positively correlated with the severity of AP (P<0.05), while AChE was lower, which was negatively correlated with the severity of AP (P<0.05). HMGB-1+AChE had a sensitivity of 48.44% and a specificity of 88.33% for diagnosing AP (P<0.05, cut-off>0.639). In addition, HMGB-1 and nutrient proteins were positively correlated, and AChE and nutrient proteins were negatively correlated in the observation group (P<0.05). The prognostic follow-up showed that the diagnostic sensitivity of HMGB-1+AChE for poor prognosis of AP was 95.65%, and the specificity was 65.85% (P<0.05).</p><p><strong>Conclusions: </strong>HMGB-1 was elevated in AP, and AChE was decreased in AP, both of which have excellent diagnostic effects on the occurrence and poor prognosis of AP.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"486-494"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of measuring the levels of ionized magnesium in the blood in critically ill patients.","authors":"Marija B Stanojević, Miro Parezanovic, Marko Biorac, Svetolik Spasić, Sanjin Kovačević, Srđan Lopičić, Ostojić Jelena Nešović","doi":"10.5937/jomb0-54782","DOIUrl":"10.5937/jomb0-54782","url":null,"abstract":"<p><strong>Background: </strong>Critical illnesses imply vital organ dysfunctions with high risk of imminent death. Magnesium is a bioessential element with multiple physiological effects relevant for vital body functions. It stabilizes excitable membranes contributing to proper neuromuscular, cardiovascular and respiratory functions. Ionized Mg2+ (iMg2+) is free bioactive form of Mg2+ in body fluids. Magnesium disorders in critically ill patients are often overlooked. Chronic Mg2+ deficiency is a condition of growing incidence in the general population and a significant factor in overall morbidity and mortality in critical illness. Main goal of this study was to investigate the existing literature related to determination of iMg2+ in the critically ill and to raise awareness of the problem of chronic Mg2+ deficiency in these patients.</p><p><strong>Methods: </strong>Search was conducted across electronic PubMed library database from 1975 to November 2024 using keywords concerning iMg2+ and critical care patients, to identify studies investigating the measuring of blood concentration of iMg2+ fraction in patients with critical health conditions. Search was limited to English language. Selection criteria included only studies on human, and excluded studies on animal population.</p><p><strong>Results: </strong>We identified 95 relevant studies. Reviewed papers show that abnormalities of Mg2+ levels are prevalent in critical illnesses. Their severity can impose vital threat. Disconcordance between total Mg2+ (tMg2+) and iMg2+ blood levels is frequently present in critical patients.</p><p><strong>Conclusions: </strong>Appropriate electrolyte analyzer assay is needed to promptly determine iMg2+ levels to assess Mg2+ status in critically ill patients, in order to help detect and correct Mg2+ imbalances and estimate the requirement for Mg2+ recompense in the case of deficiency, and thereby provide better outcome of the disease.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"386-399"},"PeriodicalIF":1.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}