Journal of Medical Biochemistry最新文献

{"title":"Oxidative stress biomarkers and chondrocyte apoptosis as predictors of postoperative outcomes in osteonecrosis of the femoral head.","authors":"Chia-Jui Hu, Ming Song, Lai Yu, Guanfei Zeng","doi":"10.5937/jomb0-61682","DOIUrl":"10.5937/jomb0-61682","url":null,"abstract":"<p><strong>Background: </strong>Osteonecrosis of the femoral head (ONFH) is a progressive disease where oxidative stress and chondrocyte apoptosis contribute to cartilage degeneration and postoperative complications. Identifying reliable biochemical markers is essential for prognosis after hip resurfacing arthroplasty (HRA).</p><p><strong>Methods: </strong>A retrospective analysis was conducted in 134 ONFH patients (ARCO stage II-III) treated with HRA. Serum oxidative stress markers-reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA)-were measured preoperatively and at serial postoperative timepoints. Chondrocyte apoptosis rates were assessed by flow cytometry. Pain and functional recovery were evaluated using VAS and Harris hip scores. Correlation, univariate, and Cox regression analyses were applied to determine associations with postoperative outcomes.</p><p><strong>Results: </strong>Patients with high oxidative stress had significantly increased ROS and MDA levels, reduced SOD activity, and elevated chondrocyte apoptosis (P&lt;0.05). These patients showed delayed functional recovery and higher complication rates. Strong correlations were observed between oxidative markers and apoptosis (ROS: r=0.912; MDA: r=0.901; SOD: r=-0.875). Cox regression identified ROS, MDA, reduced SOD, and apoptosis as independent risk factors for postoperative failure (HR range: 1.06-1.49, all P&lt;0.05).</p><p><strong>Conclusions: </strong>Serum oxidative stress markers and chondrocyte apoptosis rates are closely linked to joint recovery and complications after HRA in ONFH patients. These biochemical indicators may serve as prognostic biomarkers, supporting early identification of high-risk individuals and guiding personalized postoperative interventions.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"507-519"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leukocyte telomere dynamic change in patients with mild to moderate covid-19 during three weeks of follow-up: Relation with therapy.","authors":"Jelena Kotur-Stevuljević, Milenković Marina Roksandić, Jelena Vekić, Nemanja Dimić, Dejan Dimić, Danica Ćujić, Azra Guzonjić, Marija Gnjatović, Aleksandra Todorović, Nataša Bogavac-Stanojević","doi":"10.5937/jomb0-63678","DOIUrl":"10.5937/jomb0-63678","url":null,"abstract":"<p><strong>Background: </strong>A three-year COVID-19 pandemic revealed aspectrum of disease severity and clinical manifestations. Themost intriguing part of this phenomenon lays in inter-individual variability in COVID-19 course among patients,which is attributable to patient's age, comorbidities and general health status. Focus of this follow-up study was toassess leukocyte telomere length change in mild-to-moderate COVID-19 patients and concomitant influence ofinflammation, oxidative stress (OS), pulmonary involvementand implemented therapy on the course of the disease.</p><p><strong>Methods: </strong>Routine biochemical/haematological parameters, markers of OS (prooxidants and antioxidants), vitaminD, IgM and IgG antibodies level and relative length ofleukocyte telomeres (rLTL) were measured at three timepoints (at diagnosis, after 14 and 21 days from the diseaseonset) in blood samples of 31 consecutive COVID-19patients, with a mild (n = 16) and moderate (n = 15) form ofthe disease, treated on an outpatient basis.</p><p><strong>Results: </strong>Although the patients had reduced rLTL at baseline (median: 0.592; 25th - 75th percentiles: 0.518-0.724), it significantly increased during the follow-up(median: 0.773; 25th - 75th percentiles: 0.615-0.923; P&lt;0.01). The rate of telomere attrition was associated withthe extent of OS and pulmonary involvement. During follow-up, the burden of OS was reduced, while antioxidantdefence mechanisms were recovered. The use of antibiotics and N-acetylcysteine-propolis supplementation wasassociated with telomere lengthening.</p><p><strong>Conclusions: </strong>Results of this study revealed significant interaction between OS, inflammation and leukocyte telomerelength attrition in COVID-19. Our data suggest that rRTLcan be a biomarker that enables more precise therapy decision and accurate patient status estimation.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"609-619"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review and meta-analysis and mendelian randomization analysis of serum phosphorus, albumin, and CRP as risk factors for death in hemodialysis patients.","authors":"Xiaofen Ma, Huan Ye","doi":"10.5937/jomb0-53079","DOIUrl":"10.5937/jomb0-53079","url":null,"abstract":"<p><strong>Background: </strong>We aimed to identify the main mortality risk factors in hemodialysis patients using data from relevant cross-sectional literature. We used Mendelian randomization (MR) to assess the causality of those identified risk factors using pertinent Genome-Wide Association Study (GWAS) Single Nucleotide Polymorphism (SNP) data.</p><p><strong>Methods: </strong>Sixteen publications detailing hemodialysis-related mortality implicated 32 death-related risk factors. Based on heterogeneity testing, we utilized randomand stratified-effects models for meta-analysis. Sensitivity analysis and bias testing were used to evaluate data reliability. Mr analysis identified type-2 diabetes (T2D), serum phosphorus, serum albumin, and age as risk factors, with hematology as the outcome. Inverse-variance weighting (IVW) analysis was used in the main study. The consistency of the IVW analysis results was evaluated simultaneously using four different methods: Mr Egger regression, weighted median estimator (WME), weighted mode, and simple mode. Horizontal pleiotropy was assessed using the Mr Egger regression intercept term; heterogeneity was evaluated using Cochran's Q.</p><p><strong>Results: </strong>Using randomand stratified-effects models, a meta-analysis of 16 published articles revealed that the following factors were associated with a greater mortality risk in hemodialysis patients: T2D; serum phosphorus, albumin, and CRP; and the Charlson comorbidity index (CCI). The results were deemed reliable based on bias (P=0.1186, I2=99.53%) and sensitivity (T=0.39, df=116, P=0.6953) analyses. IVW indicated a genetic-level positive causal relationship between T2D and hematology (OR=1.2572, 95% CI=1.0375-1.5235; P=0.0195). Genetic-level serum pathology and hematology were positively correlated (OR=2.0269, 95% CI=1.0614-3.8708; P=0.0323). However, age (OR=11.1112, 95% CI=0.83) was a factor. No discernible genetic causal relationship occurred between hematology and serum ferritin (OR=0.6707, 95% CI=0.4612-0.9707; P=0.4612) or albumin (OR=1.2933, 95% CI=0.8931-1.8729; P=0.1733).</p><p><strong>Conclusions: </strong>Meta-analysis identified the number of dialysis sessions, serum CRP, CCI, T2D, serum phosphorus, and serum albumin as mortality risk factors in hemodialysis patients. The Mr results showed positive causal relationships between T2D incidence and serum phosphorus with hemodialysis risk.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"481-492"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First-trimester biochemical inflammatory markers (NHR and NLR) as predictors of preeclampsia risk.","authors":"Qianqian Wang, Xuehui Li, Xiaotao Ma","doi":"10.5937/jomb0-61798","DOIUrl":"10.5937/jomb0-61798","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia (PE) remains a leading cause of maternal and perinatal morbidity. The early identification of high-risk pregnancies requires accessible and cost-effective laboratory markers. This study aimed to evaluate first-trimester blood count-derived inflammatory indices, with a focus on the neutrophil-to-HDL ratio (NHR) and neutrophil-to-lymphocyte ratio (NLR), for PE risk prediction.</p><p><strong>Methods: </strong>A retrospective analysis was conducted involving 90 patients with PE and 90 healthy pregnant women. Routine hematological and biochemical parameters were measured using an automated hematology analyzer and an automated biochemical analyzer respectively, and derived indices (NLR, NHR, m H r, SII) were calculated. Independent risk factors were determined through multivariate logistic regression, and a predictive model was validated by receiver operating characteristic (ROC) curve analysis.</p><p><strong>Results: </strong>PE patients exhibited significantly higher NLR, NHR, and SII levels, compared with controls (all p &lt; 0.001). Multivariate logistic regression identified NLR, NHR, and body mass index (BMI) as independent predictors of PE. The combined model incorporating these factors achieved excellent predictive performance, with an area under the ROC curve of 0.909 (95% CI: 0.866-0.952).</p><p><strong>Conclusions: </strong>The first-trimester NHR and NLR, combined with BMI, constitute a clinically useful panel for early PE prediction. This readily applicable model provides a valuable alternative to cost-intensive screening methods, particularly in resource-constrained settings. Its implementation could enhance first-trimester risk stratification and guide timely interventions, ultimately contributing to improved maternal and perinatal outcomes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"601-608"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical indicators of inflammatory response in leukemia patients with picc-associated phlebitis: Evaluation of basic fibroblast growth factor combined with light therapy.","authors":"Shurong Zhang, Xiaotian Yang, Xingxia Zhang, Yan Wan, Youhuan Yu","doi":"10.5937/jomb0-61776","DOIUrl":"10.5937/jomb0-61776","url":null,"abstract":"<p><strong>Background: </strong>Peripherally inserted central catheter (PICC)associated phlebitis is a common complication in leukemia treatment, closely related to inflammatory responses. Current standard management, primarily relying on catheter removal and anti-inflammatory drugs, often demonstrates insufficient efficacy in rapidly controlling inflammation, resolving symptoms, and preventing recurrence. Moreover, there is a notable lack of objective, biomarker-driven monitoring methods to guide and evaluate therapy. This study aimed to investigate the biochemical changes in key inflammatory markers - C-reactive protein (CRP), procalcitonin (PCT), erythrocyte sedimentation rate (ESR), and interleukin-6 (IL-6) - in patients with PICC-associated phlebitis, and to evaluate their value in monitoring the efficacy of basic fibroblast growth factor (bFGF) combined with light therapy, a novel approach proposed to address these limitations.</p><p><strong>Methods: </strong>A retrospective cohort of 65 leukemia patients with PICC-associated phlebitis was analyzed. Patients were divided into an intervention group (bFGF + light therapy, n=33) and a control group (standard care, n=32). Inflammatory markers (CRP, PCT, ESR, IL-6) were measured before and after 7 days of treatment using standardized laboratory assays. Phlebitis grading, visual analogue scale (VAS) pain scores, and EORTC QLQ-C30 quality of life scores were also assessed.</p><p><strong>Results: </strong>By day 7, the intervention group showed significantly greater reductions in CRP (69.9% vs 28.2%), PCT (71.2% vs 4.4%), ESR (69.7% vs 18.9%), and IL-6 (81.1% vs 14.5%) compared with controls (all P&lt;0.001). These biochemical improvements paralleled reductions in phlebitis severity and pain, and correlated with enhanced quality of life scores.</p><p><strong>Conclusions: </strong>CRP, PCT, ESR, and IL-6 serve as sensitive biochemical indicators for evaluating treatment response in PICC-associated phlebitis. The findings highlight the clinical utility of these laboratory markers in monitoring therapeutic efficacy and suggest that bFGF combined with light therapy exerts significant anti-inflammatory effects in leukemia patients, offering a promising alternative to conventional strategies.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"493-506"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlations of P53, SLC7A11, CD3+ T cells, and CD8+ T cells with pathological features and prognosis in endometrial carcinoma: Insights from an Asian cohort.","authors":"Yuanjie Lv, Weiwei Zhou, Jingyu Zhang, Jing Feng, Rongge Xing, Zhigang Miao","doi":"10.5937/jomb0-60128","DOIUrl":"10.5937/jomb0-60128","url":null,"abstract":"<p><strong>Background: </strong>This study investigates the clinical significance of Solute Carrier Family 7 Member 11 (SLC7A11), Tumor Protein p53 (p53), c D3+ T cells, and CD8+ T cells in endometrial carcinoma (EC). By evaluating their expression patterns and correlations with histopathological parameters and patient outcomes, we aim to develop a prognostic risk model integrating these biomarkers.</p><p><strong>Methods: </strong>We enrolled 134 EC patients (observation group) diagnosed between February 2023 and January 2024 and 128 concurrent healthy controls, and were confirmed to have no endometrial abnormalities by gynecological examination and ultrasound. Quantitative and qualitative assessments were conducted to measure SLC7A11, p53, and CD3+/CD8+ T cell levels. Diagnostic performance of individual and combined markers was analyzed using receiver operating characteristic (ROC) curves, while a logistic regression-based predictive model was developed. Associations of these biomarkers with EC histopathological features and 1-year survival outcomes were further evaluated.</p><p><strong>Results: </strong>Compared to controls, EC patients exhibited notable reductions in CD3+/CD8+ T-cell infiltration but elevations in SLC7A11 and p53 expression (P&lt;0.05). According to qualitative analysis, CD3 and CD8 positivity were similarly lower in tumor tissues than in adjacent normal tissue, whereas SLC7A11 and p53 staining were more frequent in malignant lesions (P &lt;0.05). For EC diagnosis, the combined AUC of p53, SLC7A11, CD3+ T cells, and CD8+ T cells reached 0.883 (72.4% sensitivity, 88.3% specificity). Their combination also effectively predicted 1-year mortality risk (AUC = 0.889), with 82.4% sensitivity and 81.0% specificity.</p><p><strong>Conclusions: </strong>p53, SLC7A11, CD3+ T cells, and CD8+ T cells show intimate connections with EC. A diagnostic model incorporating these biomarkers enhances the accuracy of EC detection, offering improved clinical utility.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"663-670"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum oxidative stress biomarkers (MDA, SOD, GSH-PX) and IL-6/TNF-α ratio as biochemical indicators of disease progression in pediatric cariogenic pulpitis.","authors":"Mengxing Wang, Tian Xia, Ying Wang","doi":"10.5937/jomb0-61223","DOIUrl":"10.5937/jomb0-61223","url":null,"abstract":"<p><strong>Background: </strong>Cariogenic pulpitis is a common pediatric oral disease in which oxidative stress and inflammatory imbalance are increasingly recognized as important pathogenic mechanisms. Biochemical markers such as malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and the serum IL-6/TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"> α</span> ratio may provide insight into disease severity and progression.</p><p><strong>Methods: </strong>A total of 64 children with cariogenic pulpitis admitted between May 2023 and October 2024 were included, along with 64 healthy children as controls. Serum MDA, SOD, GSH-Px, and IL-6/TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"> α</span> ratio were measured using standard biochemical assays (TBA colorimetry, xanthine oxidase method, NADPH-coupled assay, and ELISA). Group comparisons were performed, and correlations with disease stage and caries depth were analyzed using Pearson correlation coefficients.</p><p><strong>Results: </strong>Compared with controls, children with pulpitis had significantly higher MDA and IL-6/TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"> α</span> levels (t = 18.542 and 20.639, both P &lt; 0.001) and lower SOD and GSH-Px activities (t = 8.279 and 12.449, both P &lt; 0.001). Within the pulpitis group (30 early stage, 24 middle stage, 10 late stage), MDA levels were positively correlated with both caries depth and disease severity (r = 0.82, P &lt; 0.05), while SOD (r = -0.78, P &lt; 0.05) and GSH-Px (r = -0.75, P &lt; 0.05) were negatively correlated. The IL-6/TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"> α</span> ratio was positively correlated with both stage and depth (r = 0.71, P &lt; 0.05), increasing significantly with lesion depth (r = 0.56, P &lt; 0.05).</p><p><strong>Conclusions: </strong>Elevated MDA and IL-6/TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"> α</span> ratio reflect greater oxidative and inflammatory burden and are associated with advanced stages of pediatric cariogenic pulpitis, whereas higher SOD and GSH-Px activities are associated with earlier disease. These biochemical indices may serve as adjunctive laboratory markers for evaluating disease progression and guiding therapeutic monitoring in pediatric pulpitis.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"620-625"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of serum NSE, and S-100β and inflammatory markers levels in epilepsy.","authors":"Zhaoxia Li, Li Su, Yi Lu, Yunqing Hu, Qian Yang","doi":"10.5937/jomb0-55343","DOIUrl":"10.5937/jomb0-55343","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To sodium valproate lore the effects of sodium valproate on serum inflammatory factors and s-100&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt; levels in patients with emergency secondary epilepsy(SE).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This was a retrospective cohort of 120 patients with SE who received Sodium valproate compared with a group who received carbamazepine for different therapeutic drugs. The general data, interleukin (IL)-2, IL-8, tumour necrosis factor (TNF)-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, neuron-specific enolase (NSE), serum acid calcium-binding protein s-100&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt;, total effective rate (TER), seizure onset condition, and adverse reactions (Ars) of the two groups were compared at baseline and at 3 months later.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;There were no significant differences in age, gender, BMI, course of the disease, stroke type, or seizure type between the study groups (P&gt; 0.05). The results showed that IL-2 of the Sodium valproate group (53.17± 4.95 &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;μ&lt;/span&gt;g/L) was lower versus Carbamazepine group (62.38± 4.83 &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;μ&lt;/span&gt;g/L) (P&lt; 0.05). The IL-8 of the Sodium valproate group postoperatively (26.48± 2.73 &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;μ&lt;/span&gt;g/L) was lower versus the Carbamazepine group (33.54± 3.39 &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;μ&lt;/span&gt;g/L) (P&lt; 0.05). Postoperatively, the TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt; in Sodium valproate group (32.18± 4.26 &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;μ&lt;/span&gt;g/L) was lower versus the Carbamazepine group (41.03± 4.92 &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;μ&lt;/span&gt;g/L) (P&lt; 0.05). The s-100&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;β&lt;/span&gt; (0.29 ± 0.15 ) &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;μ&lt;/span&gt;g/L in the Sodium valproate group was lower versus the Carbamazepine group (0.54± 0.14) &lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 2","pages":"422-428"},"PeriodicalIF":1.9,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between intestinal microbiota, inflammatory cytokines, and the expression of miR-330-3p and miR-515-5p in patients with ulcerative colitis.","authors":"Kaidi Qin, Chao Hu, Wenjun Li, Wulin Wang, Zilong Zhang","doi":"10.5937/jomb0-54789","DOIUrl":"10.5937/jomb0-54789","url":null,"abstract":"<p><strong>Background: </strong>Ulcerative colitis (UC) has a complex pathogenesis involving multiple factors. This study aims to explore the interplay among intestinal microbiota, serum inflammatory cytokines, and miR-330-3p, miR-515-5p in UC patients for novel treatment strategies.</p><p><strong>Methods: </strong>The study enrolled 95 healthy controls, 88 UC patients in remission, and 91 in active diseases. Assessments included clinical data, intestinal microbiota detection (using culture methods), serum cytokine measurement (using ELISA assay), and miRNA expression analysis (using RTqPCR). ROC curves evaluated diagnostic value, and multivariate logistic regression identified risk factors.</p><p><strong>Results: </strong>From the control group to UC remission and then to seizure UC, significant decreases were observed in Bifidobacterium, Lactobacillus counts, IL-10 levels, and miR-515-5p expression, while increases were noted in Enterobacteriaceae, Enterococcus counts, IL-6, TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> levels, and miR-330-3p expression (P&lt; 0.05). ROC curve analysis showed improved diagnostic accuracy for UC with the combination of miR-330-3p and miR-515-5p (AUC=0.899, 95% CI: 0.936 -0.978, sensitivity: 92.18%, specificity: 88.42%). miR-515-5p positively correlated with beneficial microbiota and IL-10 (r &gt; 0.5, p&lt;0.001) and negatively with harmful microbiota, IL-6, and TNF-a (r&lt; -0.5, p&lt;0.001); miR-330-3p showed opposite correlations. miR-515-5p, Bifidobacterium, Lactobacillus, and IL-10 were protective factors for UC, whereas miR-330-3p, Enterobacteriaceae, Enterococcus, IL-6, and TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> were risk factors.</p><p><strong>Conclusions: </strong>This study revealed interactions among intestinal microbiota, serum inflammatory cytokines, and miRNAs in UC patients, confirming the potential of miR-330-3p and miR-515-5p in UC diagnosis and assessment.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 2","pages":"274-282"},"PeriodicalIF":1.9,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical biochemical evaluation of trace elements and insulin-like growth factor-I in children with short stature: Diagnostic and correlative insights from the Guangzhou cohort.","authors":"Bei Huang, Xiaojun Wang, Ying Zhang","doi":"10.5937/jomb0-62146","DOIUrl":"10.5937/jomb0-62146","url":null,"abstract":"<p><strong>Background: </strong>Trace elements and insulin-like growth factor-I (IGF-I) are critical biochemical regulators of skeletal growth and endocrine function. However, limited data are available regarding their laboratory correlations in pediatric short stature. This study aimed to evaluate the biochemical profiles of calcium, magnesium, zinc, and lead, together with serum IGF-I levels, and to assess their diagnostic significance in children with growth retardation in the Guangzhou region.</p><p><strong>Methods: </strong>A total of 876 children aged 2-12 years were enrolled, including 276 with growth retardation, 300 with short stature, and 300 healthy controls. Serum trace elements and IGF-I concentrations were determined using standardized clinical chemistry assays in a hospital laboratory. Intergroup differences were analyzed using ANOVA, and multivariate logistic regression was employed to identify independent biochemical predictors of growth retardation.</p><p><strong>Results: </strong>Children with growth retardation exhibited significantly lower serum levels of calcium, magnesium, and zinc, and elevated blood lead concentrations compared with controls (P&lt; 0.05). IGF-I levels were reduced (l0 9 .1 8 ± 45.08 vs. 111.46 ± 55.44 ng/mL, P= 0.012) and correlated positively with calcium, magnesium, and zinc levels. Multivariate analysis identified low calcium, magnesium, zinc, and IGF-I, as well as elevated lead, as independent predictors of growth retardation.</p><p><strong>Conclusions: </strong>Alterations in serum trace elements and IGF-I represent measurable biochemical indicators associated with pediatric growth retardation. Integrating trace element and IGF-I assessments into routine biochemical testing may improve early diagnosis and monitoring of short stature in clinical practice.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 2","pages":"438-446"},"PeriodicalIF":1.9,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149016915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}